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Development of Green Production Technology and Research of Harpagophytum Procumbens Root Dry Extract 原藜根干提取物绿色生产技术开发与研究
Pub Date : 2021-08-31 DOI: 10.15587/2519-4852.2021.239332
A. Kriukova, D. Lytkin, M. Marchenko, I. Vladymyrova
The aim of this work is to study of standardization parameters and pharmacological properties of Harpagophytum procumbens (H. procumbens) root dry extract. Materials and methods – obtaining of H. procumbens root dry extract was performed in accordance with developed scheme that is characterized by simplicity and cost-effectiveness of technology compared to analogous scheme. Study of standardization parameters of obtained extract was performed in accordance with the requirements of the monograph «Harpagophyti extractum siccum» of the State Pharmacopoeia of Ukraine 2.2., harmonized with the monograph «Harpagophyti extractum siccum» of the European Pharmacopoeia 9.5. Pharmacology study of analgesic and anti-inflammatory activities were conducted on the rat paw formalin-induced edema model. Results. Obtained H. procumbens root dry extract is a free flowing, non-hygroscopic, light brown powder with taste and odor that is specific to raw material of H. procumbens. Determined loss on drying was 3.2±0.18 %. Content of heavy metals in dry extract was not more than 0.01 % (100 ppm). Harpagoside was identified by TLC method. Chromatographic zones on the obtained chromatograms of the reference solution and test solutions are the same in intensity of a color, cross-over and sharpness of development. Based on the results of the research performed by HPLC method, it has been established that content of harpagoside in investigated H. procumbens root dry extract is 2.50±0.02 %. According to the results of pharmacological research it was found that investigated dry extract (the content of harpagoside is not less than 37.0 mg/kg) showed a higher activity compared to reference drug («Phong Te Thap»). Conclusions. A new method of obtaining dry extract of H. procumbens (3.5:1) was proposed. The developed green production technology is simple; it does not require special equipment and expensive solvents. A correspondence of quality parameters with the requirements of State Pharmacopoeia of Ukraine and European Pharmacopoeia: appearance, identification of harpagoside and fructose, loss of drying, content of heavy metals, assay of harpagoside (2.50±0.02 %) was set. Analgesic and anti-inflammatory activities of H. procumbens dry extract has been established
本研究的目的是研究原丘茴香根干提取物的标准化参数和药理性质。根据开发的方案进行了原藜根干提取物的材料和方法的获取,与类似方案相比,该方案具有简单和技术成本效益的特点。所得提取物的标准化参数研究按照乌克兰国家药典2.2的专著«Harpagophyti extract siccum»的要求进行。,与欧洲药典9.5版的专著«Harpagophyti extract siccum»相协调。对福尔马林致大鼠足跖水肿模型进行了镇痛和抗炎作用的药理学研究。结果。所获得的原藜根干提取物是一种自由流动、不吸湿的浅棕色粉末,具有原藜原料特有的味道和气味。干燥时的测定损失为3.2±0.18%。干提取物中重金属含量不超过0.01% (100 ppm)。采用薄层色谱法鉴别。在得到的参比溶液和测试溶液的色谱图上的色谱区在颜色强度、交叉度和显影清晰度方面是相同的。HPLC法测定结果表明,所研究的原杜仲根干提取物中哈巴苷的含量为2.50±0.02%。药理研究结果表明,所研究的干提取物(哈巴苷含量不低于37.0 mg/kg)比参比药(«Phong Te Thap»)具有更高的活性。结论。提出了一种提取原杜仲干提取物(3.5:1)的新方法。发达的绿色生产技术简单;它不需要特殊设备和昂贵的溶剂。设置符合乌克兰国家药典和欧洲药典要求的质量参数:外观、哈巴果苷和果糖的鉴别、干燥损失、重金属含量、哈巴果苷含量(2.50±0.02%)。已经证实了原丘茴香干提取物的镇痛和抗炎作用
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引用次数: 3
Intensification of the Extraction Process of Phenolic Compounds from Acorus Calamus Leaves 菖蒲叶中酚类化合物的强化提取工艺研究
Pub Date : 2021-08-31 DOI: 10.15587/2519-4852.2021.238329
O. Andryushayev, O. Ruban, Yuliia Maslii, Inna Rusak
The aim. To determine the intensified method of extraction of phenolic compounds from Acorus calamus leaves and optimal conditions for the process. Materials and methods. In order to develop the optimal intensified method of extraction samples were prepared in different conditions of raw materials-extractant ratio, temperature, time and multiplicity. As a raw materials spectrophotometrically pre-standardized Acorus calamus leaves were used. The extraction was carried out in a hermetically sealed ultrasonic extraction reactor PEX 1 (REUS, Contes, France). As the criteria of extraction efficiency were indicators of dry residue and total amount of flavonoids determined using methods described in State Pharmacopoeia of Ukraine. The amount of flavonoids was determined spectrophotometrically on a certified device Specord 200 (Analytik Jena, Germany). Results. According to our research results it was found that ultrasonic action and addition of surfactant significantly improves the efficiency of the extraction process. The optimal conditions for the process were determined. Experimentally proved that the rational raw material-extractant ratio is 1:15. Comparative study of the extraction process with different temperatures showed that the highest amount of extractives is achieved at temperature 70 °C and 45 min of duration. The optimal extraction multiplicity is 3. Conclusions. As a result of the study, the intensified extraction method for Acorus calamus leaves – re-maceration with ultrasound – was established. The conducted researches allowed to develop the method of extraction, expedient in the conditions of the modern pharmaceutical industry.
的目标。确定菖蒲叶中酚类化合物的强化提取方法及最佳工艺条件。材料和方法。为了建立最佳的强化提取方法,在不同的原料与萃取剂比、温度、时间和多重度条件下制备样品。以分光光度法预标准化菖蒲叶为原料。在密封超声提取反应器PEX 1 (REUS, Contes, France)中进行提取。提取效率的标准为干燥残留量指标和总黄酮含量指标,提取方法详见乌克兰国家药典。黄酮类化合物的量在Specord 200 (Analytik Jena,德国)认证设备上分光光度法测定。结果。研究结果表明,超声作用和表面活性剂的加入显著提高了提取工艺的效率。确定了该工艺的最佳工艺条件。实验证明,合理的料萃取比为1:15。不同温度下提取工艺的对比研究表明,在温度为70℃,提取时间为45 min时,提取率最高。最优提取多重数为3。结论。在此基础上,建立了菖蒲叶强化提取方法——超声再浸渍法。所进行的研究允许开发的提取方法,方便在现代制药工业的条件下。
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引用次数: 1
Theoretical Study of the Possibility of Decamethoxin Complexes with Thiotriazolinе to Be Formed 十甲虫胺与硫三唑啉形成配合物可能性的理论研究
Pub Date : 2021-08-31 DOI: 10.15587/2519-4852.2021.239279
L. Kucherenko, S. Shishkina, Elena Chonka
Over the last decade, much attention has been paid to the prevention and treatment of chronic diseases of the oral mucosa. This is primarily due to the increase in the number of patients who seek dental care for diseases of the oral mucosa (DOM). Currently, due to the lack of special epidemiological studies, information on the pathology of the oral mucosa in the literature is almost non-existent. The etiology and pathogenesis have not been definitively elucidated. It is established that a significant role in the pathogenesis of chronic inflammatory processes belongs to the state of the microbiocenosis of the oral mucosa. One of the most striking examples of domestic drugs of the antioxidant group is thiotriazoline, developed by the staff of the NGO “Pharmatron”, as well as specialists of the Department of Pharmaceutical Chemistry of Zaporozhye State Medical University under the leadership of professor Mazur I. A. This drug has an antioxidant and membrane-stabilizing effect. The aim. The combined use of several drugs can be complicated by the formation of their supramolecular complexes. Therefore, before the introduction of several substances in it is necessary to model the possibility of intermolecular interactions between them. For this purpose, methods of quantum chemistry are used. Material and methods. We examined the structures and energy characteristics of the complexes, formed thiotriazoline and decamethoxin, also carried out analysis and established at what temperature the formation of a substance from two active components was possible. Results. As a result, the probability of the formation of intermolecular hydrogen bonds is reduced, which is very well seen in the trend of interactions between acid and morpholine. Conclusions. Quantum chemical study of a two-component system consisting of thiotriazoline and decamethoxine showed that the most energetically advantageous three-component complexes have a sufficiently low interaction energy of thiotriazoline and decamethoxine. In addition, the data that are provided in the analysis of the tree diagram suggest that in the technological process in the manufacture of dosage forms, it is advisable to use a temperature not higher than 115℃.
近十年来,口腔黏膜慢性疾病的防治受到了广泛的关注。这主要是由于因口腔黏膜疾病(DOM)而寻求牙科护理的患者人数增加。目前,由于缺乏专门的流行病学研究,文献中关于口腔黏膜病理的信息几乎为零。病因和发病机制尚未明确阐明。已经确定,在慢性炎症过程的发病机制中起重要作用的是口腔黏膜微生物病的状态。抗氧化类国产药物中最引人注目的例子之一是硫代三唑啉,它是由非政府组织“Pharmatron”的工作人员以及扎波罗热国立医科大学药物化学系的专家在Mazur I. a教授的领导下开发的,这种药物具有抗氧化和稳定膜的作用。的目标。几种药物的联合使用会因其超分子复合物的形成而变得复杂。因此,在引入几种物质之前,有必要对它们之间分子间相互作用的可能性进行建模。为此,使用了量子化学的方法。材料和方法。我们检查了配合物的结构和能量特征,形成了硫三唑啉和十甲氧胺,也进行了分析,并确定了在什么温度下,两种活性成分可能形成一种物质。结果。因此,分子间氢键形成的概率降低,这在酸与啉相互作用的趋势中可以很好地看到。结论。对硫代三唑啉和十甲基噻嗪双组分体系的量子化学研究表明,最具能量优势的三组分配合物具有足够低的硫代三唑啉和十甲基噻嗪的相互作用能。另外,从树形图分析中提供的数据可以看出,在剂型制造的工艺过程中,使用不高于115℃的温度为宜。
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引用次数: 0
Insilico and Invitro Evaluation of Dy(III) Complex on Serum Albumin as well as Amino Acid Loops in M-Protease of SARS-Coronavirus-2 Dy(III)配合物对sars -冠状病毒-2型血清白蛋白和m -蛋白酶氨基酸环的体外和体外研究
Pub Date : 2021-07-11 DOI: 10.38177/ajast.2021.5217
M. Santhiya, Lurthu Pushparaj
Countless proteins contain signal patterns that act as entry point and guiding others protein to particular cell frameworks. Likewise, the mutation in the main chain protease of “spike” proteins seemed to be crucial in viral infection and recombination in COVID-19. A certain drug that binds to the sequence will further prevent coronavirus synchronization. To address this, we designed and characterized a new complex for COVID-19 inhibition that contains 1,10-phenantroline as a ligand and Dy(III) as more than just a metal. The absorption character in neat solvent, in the existence of 4.5 percent BSA, as well as the biological interplay of COVID-19 virus via molecular docking, has been carried out to determine the complex's efficacy in this pandemic situation. The complex's absorbance spectra shows intense peaks that correspond to intra ligand π - π*, n - π*, and charge transfer transitions. It also shows a red shift in peaks corresponding to LCT and MLCT transitions in 4.5 percent of BSA. The molecular docking analysis of the complex with the COVID-19 virus (PDB: 6LU7) reveals a strong polar interaction with various amino acids in the spike protein. The complex has awesome binding energies in the -8.8 kcal mol-1 range. As a result, they are legit contender compounds for the development of candidates against SARS-COV-2. We also evaluated the complex's basic energies, such as potential and steric energy, using Gaussian energy calculations.
无数蛋白质含有信号模式,这些信号模式作为入口点,引导其他蛋白质进入特定的细胞框架。同样,“刺突”蛋白主链蛋白酶的突变似乎在COVID-19病毒感染和重组中起着至关重要的作用。与该序列结合的某种药物将进一步阻止冠状病毒的同步。为了解决这个问题,我们设计并表征了一种新的COVID-19抑制复合物,该复合物含有1,10-吩啉作为配体,而Dy(III)不仅仅是一种金属。为了确定该复合物在此次大流行疫情中的有效性,研究了该复合物在纯溶剂中,在4.5% BSA存在下的吸收特性,以及通过分子对接与COVID-19病毒的生物相互作用。该配合物的吸光度谱显示出对应于配体内π - π*、n - π*和电荷转移跃迁的强烈峰。它还显示了在4.5%的BSA中对应LCT和MLCT跃迁的峰的红移。该复合体与COVID-19病毒(PDB: 6LU7)的分子对接分析显示,该复合体与刺突蛋白中的多种氨基酸存在强极性相互作用。该配合物的结合能在-8.8 kcal mol-1范围内。因此,它们是开发抗SARS-COV-2候选药物的合法竞争者化合物。我们还利用高斯能量计算计算了配合物的基本能,如位能和位阻能。
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引用次数: 0
CNF/AgNP/chitosan Film With Antimicrobial and Antihemolytic Properties for Wound Healing CNF/AgNP/壳聚糖膜在伤口愈合中的抗菌和抗溶血作用
Pub Date : 2021-06-22 DOI: 10.2139/ssrn.3871593
P. Hasibuan, Yuandani Yuandani, M. Tanjung, S. Gea, Khatarina Meldawati Pasaribu, M. Harahap, Yurika Almanda Perangin-Angin, Andre Prayoga, Junius Gian Ginting
Cellulose nanofibers (CNF), chitosan, and silver nanoparticles (AgNP) are widely used to enhance the active functions and antibacterial properties in wound dressing. This study was conducted to prepare CNF/AgNP/chitosan incorporation by straight incorporated method and its antimicrobial activity. CNF were isolated from oil palm empty fruit bunches (OPEFB) by pulping method and acid hydrolysis. AgNP were synthesized by green synthesis method. The wound dressing was produced by mixing 10% CNF solution in LiCl/DMAc and AgNP-chitosan in a glass plate with various variations of CNF/AgNP/chitosan, i.e. 100:0, 80:20, 60:40, and 50:50. UV-visible and TEM analyses were used to confirm the formation of AgNP and CNF in nanoscale. The results showed the particles with an absorption of 435 nm and spherical shapes. Based on the calculation by using imageJ analysis, the diameters of CNF were about 50 nm and lengths of several micrometers. FTIR was used to analyze the chemical bonding of AgNP/chitosan and the incorporated the AgNP/chitosan in CNF. From XRD analysis, the presence of AgNP did not affect the crystallinity of CNF. SEM images showed that the addition of AgNP resulted in the stretching of CNF pores on the pads. Thermal degradation of the film increased with the addition of AgNP/chitosan by up to 40%. Antimicrobial test and hemocompatibility test were shown that the formed film of CNF/AgNP/chitosan successfully inhibit bacterial growth and classified as non-hemolytic materials, thus can be further studied its potential as wound dressing.
纤维素纳米纤维(CNF)、壳聚糖和纳米银(AgNP)被广泛应用于伤口敷料中,以增强其活性和抗菌性能。研究了直接包合法制备CNF/AgNP/壳聚糖包合物及其抑菌活性。采用制浆法和酸水解法从油棕空果串(OPEFB)中分离出CNF。采用绿色合成法合成AgNP。将10% CNF/AgNP/壳聚糖溶液与LiCl/DMAc溶液混合在玻璃板上,CNF/AgNP/壳聚糖的比例分别为100:0、80:20、60:40和50:50。紫外可见和透射电镜分析证实了AgNP和CNF在纳米尺度上的形成。结果表明,该粒子的吸光度为435 nm,呈球形。通过imageJ分析计算,CNF的直径约为50 nm,长度为几微米。利用红外光谱(FTIR)对AgNP/壳聚糖及其在CNF中的结合进行了分析。XRD分析表明,AgNP的存在对CNF的结晶度没有影响。SEM图像显示,AgNP的加入导致CNF孔在衬垫上的拉伸。随着AgNP/壳聚糖的加入,膜的热降解率提高了40%。抗菌试验和血液相容性试验表明,形成的CNF/AgNP/壳聚糖膜成功地抑制了细菌的生长,并被归为非溶血性材料,因此可以进一步研究其作为伤口敷料的潜力。
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引用次数: 0
Berberine-Loaded M2 Macrophage-Derived Exosomes for Spinal Cord Injury Therapy 装载小檗碱的M2巨噬细胞衍生外泌体用于脊髓损伤治疗
Pub Date : 2020-11-20 DOI: 10.2139/ssrn.3734107
Zhanshan Gao, Chuanjie Zhang, Daoyong Li, Jiaquan Lin, X. Mei, Chao Wu
Spinal cord injury (SCI) causes immune activation of resident microglia. Activated microglia have two different phenotypes, the pro-inflammatory classically activated (M1) phenotype and the anti-inflammatory alternatively activated (M2) phenotype. M1 phenotype microglia are the key factor in inflammation. The treatment of SCI remains a huge challenge due to the nontargeting and inefficiency of anti-inflammatory drugs through the blood-brain barrier (BBB). The purpose of this experiment was to design M2-type primary peritoneal macrophages exosomes (Exos) as a drug carrier for berberine (Ber), which can be efficiently targeted to deliver drugs to the injured spinal cord due to the natural advantage of Exos across the BBB. The Exos were loaded with Ber by an ultrasonic method. In vitro and in vivo experiments confirmed that the loaded sample (Exos-Ber) could decrease the M1 protein marker iNOS, elevate the M2 protein marker CD206 and reduce inflammatory and apoptotic cytokines (TNF-α, IL-1β, IL-6, Caspase 9, Caspase 8), which showed that Exos-Ber had a good anti-inflammatory and anti-apoptotic effect by inducing microglia from the M1 phenotype to M2 phenotype polarization. Moreover, the motor function of SCI mice was significantly improved after Exos-Ber treatment, indicating that Exos-Ber is a potential agent for SCI therapy.
脊髓损伤引起驻留小胶质细胞的免疫激活。活化的小胶质细胞有两种不同的表型,促炎经典活化(M1)表型和抗炎交替活化(M2)表型。M1型小胶质细胞是炎症的关键因素。由于抗炎药物通过血脑屏障(BBB)的非靶向性和效率低下,脊髓损伤的治疗仍然是一个巨大的挑战。本实验的目的是设计m2型原代腹膜巨噬细胞外泌体(Exos)作为小檗碱(Ber)的药物载体,由于Exos跨越血脑屏障的天然优势,可以有效地靶向将药物递送到损伤的脊髓。用超声波的方法在Exos上装载了Ber。体外和体内实验证实,负载样品(Exos-Ber)可降低M1蛋白标记物iNOS,升高M2蛋白标记物CD206,降低炎症和凋亡细胞因子(TNF-α、IL-1β、IL-6、Caspase 9、Caspase 8),表明Exos-Ber通过诱导小胶质细胞从M1表型向M2表型极化,具有良好的抗炎和抗凋亡作用。此外,Exos-Ber治疗后,脊髓损伤小鼠的运动功能明显改善,表明Exos-Ber是一种潜在的脊髓损伤治疗药物。
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引用次数: 14
Extracellular Vesicle-Based Therapeutics for the Regeneration of Chronic Wounds: Current Knowledge and Future Perspectives 基于细胞外囊泡的慢性伤口再生疗法:目前的知识和未来的展望
Pub Date : 2020-11-05 DOI: 10.2139/ssrn.3681038
P. Lou, Shuyun Liu, Xuewen Xu, Cheng Pan, Yanrong Lu, Jingping Liu
Chronic wounds are still an intractable medical problem for both clinicians and researchers and cause a substantial social and medical burden. Current clinical approaches can only manage wounds but have limited capacity to promote the regeneration of chronic wounds. As a type of natural nanovesicle, extracellular vesicles (EVs) from multiple cell types (e.g., stem cells, immune cells, and skin cells) have been shown to participate in all stages of skin wound healing including inflammation, proliferation, and remodeling, and display beneficial roles in promoting wound repair. Moreover, EVs can be further re-engineered with genetic/chemical or scaffold material-based strategies for enhanced skin regeneration. In this review, we provide an overview of EV biology and discuss the current findings regarding the roles of EVs in chronic wound healing, particularly in immune regulation, cell proliferation and migration, angiogenesis, and extracellular matrix remodeling, as well as the therapeutic effects of EVs on chronic wounds by genetic modification, in combination with functionalized biomaterials, and as drug carriers. We also discuss the challenges and perspectives of translating EV-based therapies into clinical wound care in the future.
对于临床医生和研究人员来说,慢性伤口仍然是一个棘手的医学问题,并造成了巨大的社会和医疗负担。目前的临床方法只能处理伤口,但促进慢性伤口再生的能力有限。作为一种天然纳米囊泡,来自多种细胞类型(如干细胞、免疫细胞和皮肤细胞)的细胞外囊泡(EVs)已被证明参与皮肤伤口愈合的所有阶段,包括炎症、增殖和重塑,并在促进伤口修复中发挥有益作用。此外,电动汽车可以通过基因/化学或基于支架材料的策略进一步重新设计,以增强皮肤再生。在这篇综述中,我们提供了EV生物学的概述,并讨论了EV在慢性伤口愈合中的作用,特别是在免疫调节、细胞增殖和迁移、血管生成和细胞外基质重塑方面的最新发现,以及EV通过基因改造、与功能化生物材料结合以及作为药物载体对慢性伤口的治疗作用。我们还讨论了未来将基于ev的治疗方法转化为临床伤口护理的挑战和前景。
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引用次数: 35
Dual Stimuli-Responsive MOF-Based Nanosystem for Synergistic Photothermal/Pharmacological Antibacterial Therapy 基于mof的双刺激响应纳米系统协同光热/药物抗菌治疗
Pub Date : 2020-10-05 DOI: 10.2139/ssrn.3692009
Ya Xiao, Meng-Ran Xu, Na Lv, Chen Cheng, Pei Huang, Jiabin Li, Yi Hu, Ming Sun
The serious threat of drug-resistant bacterial pathogens has arisen through overuse of antibiotics. Photothermal therapy (PTT) has come to prominence as viable alternative strategy for antibacterial therapy. In this work, we report a NIR/pH dual stimuli-responsive antibacterial formulation based on zeolitic imidazolate frameworks-8 (ZIF-8) with strong antibacterial activity that combines photothermal heating with enhanced antibiotic delivery. ZIF-8 with polydopamine (PDA) surface modification was used to encapsulate the antibiotic vancomycin to construct a novel antimicrobial formulation (Van@ZIF-8@PDA). This treatment was tested against Gram-positive vancomycin-resistant Staphylococcus aureus Mu50. Results showed that the PDA coating improved ZIF-8 stability and dispersion, while also conferring a high photothermal conversion efficiency. Hyperthermia activated by near-infrared (NIR) light irradiation, in conjunction with pH-dependent nanoparticle degradation to release vancomycin, enabled tight control of drug delivery that functioned synergistically in the elimination of both planktonic bacteria prior to biofilm formation and established biofilms. We found that this combined formulation compromises cell structure while also degrading bacterial DNA. Moreover, further investigation showed that the Van@ZIF-8@PDA nanoparticles exhibit excellent biocompatibility, with low toxicity toward host organs and tissues, while also reducing the antibiotic concentration needed for effective bacterial control. Finally, we treated Mu50 in a mouse model of skin abscess and found that Van@ZIF-8@PDA was effective and safe in vivo. Cumulatively, this study shows that this NIR/pH dual stimuli-responsive nanoparticle-based formulation offers a promising potential strategy for clinical application against bacterial infection that circumvents antibiotic resistance.
耐药细菌病原体的严重威胁是由于过度使用抗生素而产生的。光热疗法(PTT)已成为突出的可行的替代策略,抗菌治疗。在这项工作中,我们报道了一种基于咪唑酸分子筛框架-8 (ZIF-8)的NIR/pH双刺激响应抗菌制剂,该制剂具有很强的抗菌活性,将光热加热与增强抗生素递送相结合。采用聚多巴胺(PDA)表面修饰的ZIF-8包封万古霉素,构建新型抗菌制剂(Van@ZIF-8@PDA)。这种治疗方法对革兰氏阳性耐万古霉素金黄色葡萄球菌Mu50进行了试验。结果表明,PDA涂层改善了ZIF-8的稳定性和分散性,同时具有较高的光热转换效率。近红外(NIR)光照射激活的热疗,结合ph依赖性纳米颗粒降解释放万古霉素,能够严格控制药物输送,在生物膜形成之前和生物膜形成之前协同消除浮游细菌。我们发现这种组合制剂在破坏细胞结构的同时也降解细菌DNA。此外,进一步的研究表明Van@ZIF-8@PDA纳米颗粒具有良好的生物相容性,对宿主器官和组织的毒性低,同时也降低了有效控制细菌所需的抗生素浓度。最后,我们在小鼠皮肤脓肿模型中处理Mu50,发现Van@ZIF-8@PDA在体内有效且安全。总的来说,这项研究表明,这种基于NIR/pH双重刺激反应的纳米颗粒配方为临床应用提供了一种有希望的潜在策略,可以对抗细菌感染,从而绕过抗生素耐药性。
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引用次数: 1
From Prevention to Diagnosis and Treatment: Biomedical Applications of Metal Nanoparticle-Hydrogel Composites 从预防到诊断和治疗:金属纳米颗粒-水凝胶复合材料的生物医学应用
Pub Date : 2020-09-24 DOI: 10.2139/ssrn.3697765
Aaron J. Clasky, Jeffrey Watchorn, Paul Z. Chen, F. Gu
Recent advances in biomaterials integrate metal nanoparticles with hydrogels to generate composite materials that exhibit new or improved properties. By precisely controlling the composition, arrangement and interactions of their constituents, these hybrid materials facilitate biomedical applications through myriad approaches. In this work we seek to highlight three popular frameworks for designing metal nanoparticle-hydrogel hybrid materials for biomedical applications. In the first approach, the properties of metal nanoparticles are incorporated into a hydrogel matrix such that the composite is selectively responsive to stimuli such as light and magnetic flux, enabling precisely activated therapeutics and self-healing biomaterials. The second approach mediates the dynamic reorganization of metal nanoparticles based on environment-directed changes in hydrogel structure, leading to chemosensing, microbial and viral detection, and drug-delivery capabilities. In the third approach, the hydrogel matrix spatially arranges metal nanoparticles to produce metamaterials or passively enhance nanoparticle properties to generate improved substrates for biomedical applications including tissue engineering and wound healing. This article reviews the construction, properties and biomedical applications of metal nanoparticle-hydrogel composites, with a focus on how they help to prevent, diagnose and treat diseases. Discussion includes how the composites lead to new or improved properties, how current biomedical research leverages these properties and the emerging directions in this growing field.
生物材料的最新进展是将金属纳米颗粒与水凝胶结合在一起,产生具有新的或改进的性能的复合材料。通过精确控制其成分的组成、排列和相互作用,这些混合材料通过无数的方法促进了生物医学应用。在这项工作中,我们试图强调设计用于生物医学应用的金属纳米颗粒-水凝胶混合材料的三种流行框架。在第一种方法中,将金属纳米颗粒的特性整合到水凝胶基质中,使得复合材料对诸如光和磁通量等刺激有选择性地响应,从而实现精确激活治疗和自愈生物材料。第二种方法是基于水凝胶结构的环境导向变化介导金属纳米颗粒的动态重组,从而实现化学感应、微生物和病毒检测以及药物递送能力。在第三种方法中,水凝胶基质在空间上排列金属纳米颗粒来产生超材料或被动增强纳米颗粒的特性,以产生用于生物医学应用的改进底物,包括组织工程和伤口愈合。本文综述了金属纳米颗粒-水凝胶复合材料的结构、性能及其在生物医学上的应用,重点介绍了金属纳米颗粒-水凝胶复合材料在疾病预防、诊断和治疗方面的应用。讨论包括复合材料如何导致新的或改进的性能,当前的生物医学研究如何利用这些性能以及这个不断发展的领域的新兴方向。
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引用次数: 42
Injectable and Sprayable Albumin/Serum Hydrogels that Demonstrate the Complete Coverage of a Wound and Possess Dynamically and Spatially Controllable Material Properties for the Prevention of Post-Operative Adhesion 可注射和喷雾的白蛋白/血清水凝胶,可完全覆盖伤口,并具有动态和空间可控的材料特性,可预防术后粘连
Pub Date : 2020-01-21 DOI: 10.2139/ssrn.3518166
S. Mao, Weicai Yan, Hui-wen Peng, Chi-Shuo Chen, Ying-Chieh Chen
Since the degree of severity and geometry of wounds vary, it is necessary to prepare an anti-adhesive hydrogel that possesses dynamically controllable material properties, exhibits biodegradability, and possesses drug-releasing properties. Injectable, sprayable, oxygen peroxide (H2O2)-sensitive and photo-cross-linkable hydrogels that permit in situ dynamic and spatial control of their physicochemical properties were synthesized for the prevention of postoperative adhesion. Albumin is the most abundant protein in blood serum and serves as a carrier for a number of molecules that exhibit poor water solubility. It is therefore a suitable biomaterial for the fabrication of hydrogels since it presents a low risk of life-threatening complications and does not require immunosuppressive therapy for preventing graft rejection. As micromolar concentrations of endogenous H2O2 are produced after tissue damage, aqueous pre-polymer solutions that exhibit solution-to-gel transition behaviors in response to H2O2 were developed. These solutions form a thin layer of hydrogel that fully covers the wound site and acts as a barrier. The physicochemical properties of this hydrogel can then be spatially post-adjusted via transdermal exposure to light to release drugs, depending on what is required for the particular injury. A significant reduction in postoperative peritoneal adhesion was observed in an animal model involving severe sidewall and bowel abrasions. This study demonstrated that the fabricated dually cross-linked, albumin-based hydrogels have great potential in such applications because they showed a low immune response, easy handling, full wound coverage, and tunable biodegradability. Precise spatial and controllable drug-release profiles may also be achieved via in situ transdermal post-tuning of the biomaterials, depending on the injury.
由于伤口的严重程度和几何形状各不相同,因此有必要制备一种具有动态可控材料特性,具有生物降解性和药物释放特性的抗粘附水凝胶。合成了可注射、可喷涂、对过氧化氧(H2O2)敏感、可光交联的水凝胶,这些水凝胶允许对其物理化学性质进行原位动态和空间控制,以防止术后粘连。白蛋白是血清中含量最多的蛋白质,是许多水溶性较差分子的载体。因此,它是一种适合制造水凝胶的生物材料,因为它具有低风险的危及生命的并发症,并且不需要免疫抑制治疗来防止移植排斥。由于组织损伤后会产生微摩尔浓度的内源性H2O2,因此研究人员开发出了在H2O2作用下表现出溶液到凝胶转变行为的水性预聚合物溶液。这些溶液形成一层薄薄的水凝胶,完全覆盖伤口,起到屏障的作用。这种水凝胶的物理化学性质可以根据特定损伤的需要,通过透皮暴露在光线下释放药物,在空间上进行后期调整。在涉及严重侧壁和肠道磨损的动物模型中,观察到术后腹膜粘连显著减少。该研究表明,制备的双交联白蛋白基水凝胶具有很大的应用潜力,因为它们具有低免疫反应,易于处理,完全覆盖伤口和可调节的生物降解性。精确的空间和可控的药物释放谱也可以通过生物材料的原位透皮后调整来实现,具体取决于损伤。
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引用次数: 1
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MatSciRN: Drug Delivery (Topic)
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