Pub Date : 2019-01-01DOI: 10.36648/2472-1646.5.1.57
Joe E Mouawad, J. Azar, Marcel Bassil
Background: Oxidative stress is an imbalance between reactive species and antioxidants production, leading to protein, DNA and lipid damage. The aim of this study was to evaluate the oxidative stress levels in buccal cells of ten non-shisha and heavy shisha smokers using MAWI iSWAB protein tubes. Materials and findings: The presence of S-glutathionylation, NOX2 and iNOS was analyzed in buccal cells of ten non-smokers and ten heavy shisha smokers using MAWI i-SWAB protein tubes, knowing that they were able to detect previously several protein oxidation biomarkers (S-nitrosylation and nitrotyrosine). Proteins concentrations were assessed by Bradford assay then western blot method was used to analyze the presence of the different proteins of interest. Conclusion: Results confirmed the findings about the ability of these tubes to detect S-nitrosylation, nitrotyrosine, as well as S-glutathionylation. Results showed also the presence of immature NOX2 in human buccal cells equally. Levels of iNOS were the same in non-shisha and heavy shisha smokerss.
{"title":"Waterpipe Smoke Effect on Oxidative Stress Levels in Buccal Cells Using MAWI i-SWAB Tubes","authors":"Joe E Mouawad, J. Azar, Marcel Bassil","doi":"10.36648/2472-1646.5.1.57","DOIUrl":"https://doi.org/10.36648/2472-1646.5.1.57","url":null,"abstract":"Background: Oxidative stress is an imbalance between reactive species and antioxidants production, leading to protein, DNA and lipid damage. The aim of this study was to evaluate the oxidative stress levels in buccal cells of ten non-shisha and heavy shisha smokers using MAWI iSWAB protein tubes. Materials and findings: The presence of S-glutathionylation, NOX2 and iNOS was analyzed in buccal cells of ten non-smokers and ten heavy shisha smokers using MAWI i-SWAB protein tubes, knowing that they were able to detect previously several protein oxidation biomarkers (S-nitrosylation and nitrotyrosine). Proteins concentrations were assessed by Bradford assay then western blot method was used to analyze the presence of the different proteins of interest. Conclusion: Results confirmed the findings about the ability of these tubes to detect S-nitrosylation, nitrotyrosine, as well as S-glutathionylation. Results showed also the presence of immature NOX2 in human buccal cells equally. Levels of iNOS were the same in non-shisha and heavy shisha smokerss.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88754542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.36648/2472-1646.5.1.56
E. Stenner, R. Russo, M. Ruscio, G. Barbati, A. Aleksova
Aim of the study: To measure high-sensitive troponin (Access hsTnI) interidentical- instrument bias (DxI800 Beckman Coulter) in order to understand if it can compromise the interpretation of absolute delta value for rapid algorithms 0/1-0/3 hours. Materials and methods: One hundred fifty-nine lithium/heparin plasma samples were processed sequentially on three DxI800 (DxI1, DxI2, DxI3). The results given by the three instruments were analyzed as followed: DxI1 vs. DxI2, DxI1 vs. DxI3, DxI2 vs. DxI3. Statistical analysis was done using the Passing-Bablok regression, Bland-Altman test, and Cohen’s Kappa statistic. Results: PB regression did not show any significant deviation from linearity and no proportional nor constant differences were observed among instruments. Moreover, the mean absolute bias, even though among the three instruments the lowest 95%CI lower limit was -3.75 and the highest 95%CI upper limit was 3.92 ng/L, was within the acceptance limits (all results
{"title":"Could the Intra-Laboratory Inter-Identical-Instrument Bias Compromise the Interpretation of the Absolute High-Sensitive Troponin Delta Around the 99th Percentile Upper Reference Limit?","authors":"E. Stenner, R. Russo, M. Ruscio, G. Barbati, A. Aleksova","doi":"10.36648/2472-1646.5.1.56","DOIUrl":"https://doi.org/10.36648/2472-1646.5.1.56","url":null,"abstract":"Aim of the study: To measure high-sensitive troponin (Access hsTnI) interidentical- instrument bias (DxI800 Beckman Coulter) in order to understand if it can compromise the interpretation of absolute delta value for rapid algorithms 0/1-0/3 hours. Materials and methods: One hundred fifty-nine lithium/heparin plasma samples were processed sequentially on three DxI800 (DxI1, DxI2, DxI3). The results given by the three instruments were analyzed as followed: DxI1 vs. DxI2, DxI1 vs. DxI3, DxI2 vs. DxI3. Statistical analysis was done using the Passing-Bablok regression, Bland-Altman test, and Cohen’s Kappa statistic. Results: PB regression did not show any significant deviation from linearity and no proportional nor constant differences were observed among instruments. Moreover, the mean absolute bias, even though among the three instruments the lowest 95%CI lower limit was -3.75 and the highest 95%CI upper limit was 3.92 ng/L, was within the acceptance limits (all results<reference change value). The concordance between each couple of instruments was mostly strong. Conclusion: Our data suggest that inter-identical-instrument bias needs to be considered before evaluating the clinical diagnostic accuracy of one absolute delta with respect to another, in order to define the minimum absolute delta that the laboratory can guarantee to the clinicians.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81772815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.36648/2472-1646.5.2.60
Yongjin Tang, Jinyuan Zheng, Xiaomeng Fu, Yang Chen, Donghong Lin
Background: Chromosomal translocations of the mixed-lineage leukemia gene (MLL; tMLL) correlate with resistance to therapy and an extremely poor prognosis for individuals with Acute Myeloid Leukemia (AML). The underlying mechanisms are still unknown. This study aims to identify the key genes and the potential molecular mechanisms involved in MLL rearrangement in AML using a bioinformatics approach. Methods: The gene expression profiles from 15 individuals with partial tandem duplication of the MLL gene (MLL-PTD)-AML and 10 tMLL-AML samples were downloaded from the Gene Expression Omnibus (GEO) database. The Differentially Expressed Genes (DEGs) were selected and functional enrichment analyses were performed. The Protein-Protein Interaction (PPI) network was established and visualized in Cytoscape. The hub genes were identified by CytoHubba and significant modules were screened out by Molecular Complex Detection (MCODE). Results: We categorized a total of 885 DEGs comprising 330 upregulated and 555 downregulated genes. The majority of DEGs were significantly enriched for calcium ion transmembrane transport, embryonic skeletal system morphogenesis and cell proliferation processes. Several pathways were enriched, including those associated with PI3K-Akt signaling and insulin resistance. We identified 32 hub genes and screened out 2 modules. Conclusion: The genes we have identified in this study may represent potential biomarkers for MLL-rearranged AML and contribute to the development of novel therapeutic strategies.
背景:混合谱系白血病基因(MLL;tMLL)与急性髓系白血病(AML)患者的治疗耐药性和极差预后相关。其潜在机制尚不清楚。本研究旨在利用生物信息学方法确定AML中涉及MLL重排的关键基因和潜在的分子机制。方法:从gene expression Omnibus (GEO)数据库下载15例MLL基因部分串联重复(MLL- ptd)-AML和10例tml -AML样本的基因表达谱。选择差异表达基因(DEGs)并进行功能富集分析。建立蛋白-蛋白相互作用(PPI)网络，并在Cytoscape中可视化。利用CytoHubba对中心基因进行鉴定，利用分子复合物检测(Molecular Complex Detection, MCODE)筛选出重要模块。结果:我们共分类了885个deg，其中包括330个上调基因和555个下调基因。大多数deg在钙离子跨膜转运、胚胎骨骼系统形态发生和细胞增殖过程中显著富集。包括与PI3K-Akt信号传导和胰岛素抵抗相关的信号通路在内的一些信号通路被富集。共鉴定出32个枢纽基因，筛选出2个模块。结论:我们在本研究中鉴定的基因可能代表mll重排AML的潜在生物标志物，并有助于开发新的治疗策略。
{"title":"Bioinformatics Analysis of Differentially Expressed Genes and Their Functional Enrichment in Acute Myeloid Leukemia Bearing MLL Translocation","authors":"Yongjin Tang, Jinyuan Zheng, Xiaomeng Fu, Yang Chen, Donghong Lin","doi":"10.36648/2472-1646.5.2.60","DOIUrl":"https://doi.org/10.36648/2472-1646.5.2.60","url":null,"abstract":"Background: Chromosomal translocations of the mixed-lineage leukemia gene (MLL; tMLL) correlate with resistance to therapy and an extremely poor prognosis for individuals with Acute Myeloid Leukemia (AML). The underlying mechanisms are still unknown. This study aims to identify the key genes and the potential molecular mechanisms involved in MLL rearrangement in AML using a bioinformatics approach. Methods: The gene expression profiles from 15 individuals with partial tandem duplication of the MLL gene (MLL-PTD)-AML and 10 tMLL-AML samples were downloaded from the Gene Expression Omnibus (GEO) database. The Differentially Expressed Genes (DEGs) were selected and functional enrichment analyses were performed. The Protein-Protein Interaction (PPI) network was established and visualized in Cytoscape. The hub genes were identified by CytoHubba and significant modules were screened out by Molecular Complex Detection (MCODE). Results: We categorized a total of 885 DEGs comprising 330 upregulated and 555 downregulated genes. The majority of DEGs were significantly enriched for calcium ion transmembrane transport, embryonic skeletal system morphogenesis and cell proliferation processes. Several pathways were enriched, including those associated with PI3K-Akt signaling and insulin resistance. We identified 32 hub genes and screened out 2 modules. Conclusion: The genes we have identified in this study may represent potential biomarkers for MLL-rearranged AML and contribute to the development of novel therapeutic strategies.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88931056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.36648/2472-1646.5.1.58
Y. Kikuchi, T. Yoshikawa, T. Asakawa, M. Takano
Approximately 50%-80%of cancer patients have been reported to result in a devasting syndrome such as cancer cachexia (CC) and at least 20% patients reach the death [1]. Sarcopenia accompanying with muscle loss shows the most clinically relevant phenotypic feature of CC, such as asthenia, fatigue, impaired physiologic function, reduced tolerance to treatments, resulting in impaired quality of life and shorter survival. Such wasting syndromes as sarcopenia are shown in 20%-70% cases depending on the tumor type. Accordingly, reversion of CC and sarcopenia is of most importance and interest. In addition, high serum IL-6 has been reported to result in cancer metastases, invasion and resulting in CC [2,3]. Early detection of such sarcopenia syndromes is clinically most important. If sarcopenia can be precedently predicted, better treatment strategies will be possible. It is well-known that sarcopenia is accompanied by increased levels of inflammation factors TNF-alpha and IL-6 [4]. It has been reported that activation of the IL-6/STAT3 pathway also plays a causative role in the pathogenesis of cancer cachexia, one of the most distressing complications associated with the development of ovarian cancer [5]. Indeed, cachexia usually accompanies the development of ascites and chemoresistance in the most advanced stages of the disease [6]. The majority of advanced gynecologic cancer (GC) patients develop cachexia, which is a major contributor of morbidity and mortality in these patients. Cachexia is primarily responsible for body and muscle weight loss and correlates with tumor burden, increased proinflammatory cytokine levels, fatigue, and reduced response to chemotherapy and radio-therapy [7]. Patients presenting with advanced stage GC often show large tumor and ascites burden that, in turn, results into severe malnourishment because of decreased oral intake and compromised bowel functions. Despite its significant negative impact on quality of life, no effective treatment is currently available for GC-related cachexia. Indeed, muscle loss and low skeletal muscle attenuation are often detected in women undergoing primary debulking surgery for the treatment of GCs. Along the same line, recent evidence suggests that baseline sarcopenia represents one of the most accurate prognostic factors for survival in advanced GC and during chemotherapy treatment [8]. Clinically, we find out that elevation of serum IL-6 in patients with gynecologic cancers is varied by effect of the adjuvant chemotherapy.
{"title":"Serum IL-6 Can Be a Sentinel Biomarker for Sarcopenia and Cancer Cachexia","authors":"Y. Kikuchi, T. Yoshikawa, T. Asakawa, M. Takano","doi":"10.36648/2472-1646.5.1.58","DOIUrl":"https://doi.org/10.36648/2472-1646.5.1.58","url":null,"abstract":"Approximately 50%-80%of cancer patients have been reported to result in a devasting syndrome such as cancer cachexia (CC) and at least 20% patients reach the death [1]. Sarcopenia accompanying with muscle loss shows the most clinically relevant phenotypic feature of CC, such as asthenia, fatigue, impaired physiologic function, reduced tolerance to treatments, resulting in impaired quality of life and shorter survival. Such wasting syndromes as sarcopenia are shown in 20%-70% cases depending on the tumor type. Accordingly, reversion of CC and sarcopenia is of most importance and interest. In addition, high serum IL-6 has been reported to result in cancer metastases, invasion and resulting in CC [2,3]. Early detection of such sarcopenia syndromes is clinically most important. If sarcopenia can be precedently predicted, better treatment strategies will be possible. It is well-known that sarcopenia is accompanied by increased levels of inflammation factors TNF-alpha and IL-6 [4]. It has been reported that activation of the IL-6/STAT3 pathway also plays a causative role in the pathogenesis of cancer cachexia, one of the most distressing complications associated with the development of ovarian cancer [5]. Indeed, cachexia usually accompanies the development of ascites and chemoresistance in the most advanced stages of the disease [6]. The majority of advanced gynecologic cancer (GC) patients develop cachexia, which is a major contributor of morbidity and mortality in these patients. Cachexia is primarily responsible for body and muscle weight loss and correlates with tumor burden, increased proinflammatory cytokine levels, fatigue, and reduced response to chemotherapy and radio-therapy [7]. Patients presenting with advanced stage GC often show large tumor and ascites burden that, in turn, results into severe malnourishment because of decreased oral intake and compromised bowel functions. Despite its significant negative impact on quality of life, no effective treatment is currently available for GC-related cachexia. Indeed, muscle loss and low skeletal muscle attenuation are often detected in women undergoing primary debulking surgery for the treatment of GCs. Along the same line, recent evidence suggests that baseline sarcopenia represents one of the most accurate prognostic factors for survival in advanced GC and during chemotherapy treatment [8]. Clinically, we find out that elevation of serum IL-6 in patients with gynecologic cancers is varied by effect of the adjuvant chemotherapy.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85083314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.2174/1875318301808010042
K. Dahiya, Rajesh Kumar, R. Dhankhar, M. Verma, A. Kumari, P. Roy, D. Dalal, V. Ghalaut, K. Chugh
Ischemia Modified Albumin (IMA) is considered as an early marker of ischemia. Its levels may be altered in any clinical condition where an ischemic event is involved. Skeletal muscle ischemia is associated with severe exercise but may also occur in moderate form of exercise.The levels of IMA were estimated before and after thirty minutes of moderate exercise in students undergoing athletic training.The present study was conducted on 120 young adult students undergoing athletic training program in the physical education department. All the subjects were healthy with normal body mass index and blood pressure. Their serum samples were collected before and after running for half an hour on the racing track and were analyzed for IMA colorimetrically and the data was subjected to appropriate statistical analysis.The levels of IMA were found to be statistically significantly higher after exercise as compared to those before exercise (p = 0.005). The ratio of IMA to albumin (IMAR) was also found to be significantly higher after exercise as compared to that before exercise (p=0.000).It may be concluded that the skeletal muscle ischemia induced by moderate aerobic exercise is associated with an increased conversion of albumin to IMA.
{"title":"Status of Ischemia Modified Albumin in Athletes Before and After Moderate Exercise","authors":"K. Dahiya, Rajesh Kumar, R. Dhankhar, M. Verma, A. Kumari, P. Roy, D. Dalal, V. Ghalaut, K. Chugh","doi":"10.2174/1875318301808010042","DOIUrl":"https://doi.org/10.2174/1875318301808010042","url":null,"abstract":"Ischemia Modified Albumin (IMA) is considered as an early marker of ischemia. Its levels may be altered in any clinical condition where an ischemic event is involved. Skeletal muscle ischemia is associated with severe exercise but may also occur in moderate form of exercise.The levels of IMA were estimated before and after thirty minutes of moderate exercise in students undergoing athletic training.The present study was conducted on 120 young adult students undergoing athletic training program in the physical education department. All the subjects were healthy with normal body mass index and blood pressure. Their serum samples were collected before and after running for half an hour on the racing track and were analyzed for IMA colorimetrically and the data was subjected to appropriate statistical analysis.The levels of IMA were found to be statistically significantly higher after exercise as compared to those before exercise (p = 0.005). The ratio of IMA to albumin (IMAR) was also found to be significantly higher after exercise as compared to that before exercise (p=0.000).It may be concluded that the skeletal muscle ischemia induced by moderate aerobic exercise is associated with an increased conversion of albumin to IMA.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48428341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.2174/1875318301808010034
J. Martínez-Martos, María E. Pulido-Navas, M. J. Ramírez-Expósito
L-arginine is an amino acid that can be metabolized to form several bioactive molecules including Nitric Oxide (NO). In the Central Nervous System (CNS), NO regulates various and important physiological functions. However, the involvement of L-arginine metabolism –and by extension of NO- in Alzheimer’s disease (AD) pathogenesis, has been suggested. To determine the Global L-Arginine Bioavailability Ratio (GABR) and NO levels (as the sum of nitrates and nitrites, NOx) in the plasma of early-stage Alzheimer’s Disease (AD) patients in order to analyze if GABR can reflect an altered NO production, to confirm the importance of L-arginine metabolism in the development of the disease, and to evaluate the putative diagnostic/prognostic value of GABR. GABR index is an indicator of the availability of L-arginine to form NO by nitric oxide synthases. It is calculated as the ratio between the levels of L-arginine and the sum of the levels of L-ornithine and L-citrulline. Plasma amino acids are measured by high-performance liquid chromatography coupled to fluorescence detection. Nitric oxide is measured in plasma as the sum of nitrates and nitrites (NOx). No changes were found in L-arginine levels, whereas L-citrulline and L-ornithine levels were highly increased in AD patients. We also found that GABR decreased significantly by 47.8% in AD patients, whereas NOx levels increased significantly by 46.9%. Receiver Operator Characteristic (ROC) curve analysis for GABR showed a sensitivity of 78.1 and a specificity of 90.5. Low plasma GABR levels in AD patients reflect that the L-arginine-NO pathway has turned towards NO in AD, probably being related to the nitroxidative stress involved in neurodegenerative diseases. Furthermore, increased NOx could also be involved in several altered physiological functions. Therefore, GABR is proposed as a putative useful biomarker of the disease.
{"title":"Altered Plasma Global Arginine Bioavailability Ratio in Early-stage Alzheimer’s Disease","authors":"J. Martínez-Martos, María E. Pulido-Navas, M. J. Ramírez-Expósito","doi":"10.2174/1875318301808010034","DOIUrl":"https://doi.org/10.2174/1875318301808010034","url":null,"abstract":"\u0000 \u0000 L-arginine is an amino acid that can be metabolized to form several bioactive molecules including Nitric Oxide (NO). In the Central Nervous System (CNS), NO regulates various and important physiological functions. However, the involvement of L-arginine metabolism –and by extension of NO- in Alzheimer’s disease (AD) pathogenesis, has been suggested.\u0000 \u0000 \u0000 \u0000 To determine the Global L-Arginine Bioavailability Ratio (GABR) and NO levels (as the sum of nitrates and nitrites, NOx) in the plasma of early-stage Alzheimer’s Disease (AD) patients in order to analyze if GABR can reflect an altered NO production, to confirm the importance of L-arginine metabolism in the development of the disease, and to evaluate the putative diagnostic/prognostic value of GABR.\u0000 \u0000 \u0000 \u0000 GABR index is an indicator of the availability of L-arginine to form NO by nitric oxide synthases. It is calculated as the ratio between the levels of L-arginine and the sum of the levels of L-ornithine and L-citrulline. Plasma amino acids are measured by high-performance liquid chromatography coupled to fluorescence detection. Nitric oxide is measured in plasma as the sum of nitrates and nitrites (NOx).\u0000 \u0000 \u0000 \u0000 No changes were found in L-arginine levels, whereas L-citrulline and L-ornithine levels were highly increased in AD patients. We also found that GABR decreased significantly by 47.8% in AD patients, whereas NOx levels increased significantly by 46.9%. Receiver Operator Characteristic (ROC) curve analysis for GABR showed a sensitivity of 78.1 and a specificity of 90.5.\u0000 \u0000 \u0000 \u0000 Low plasma GABR levels in AD patients reflect that the L-arginine-NO pathway has turned towards NO in AD, probably being related to the nitroxidative stress involved in neurodegenerative diseases. Furthermore, increased NOx could also be involved in several altered physiological functions. Therefore, GABR is proposed as a putative useful biomarker of the disease.\u0000","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47755668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-20DOI: 10.2174/1875318301808010017
Gerrard Gabriela, Martí M. Belén, Diviani Romina, C. M. Jose, Lioi Susana, Beloscar Juan, D. Mabel
The fact that only part of the population that lives in endemic areas gets Chagas disease and that only some of the patients with chronic infection develop symptoms, supports the importance of investigating the factors of each host in the susceptibility and the development of the disease. Chronic pathological processes and progressive inflammation lead to alterations in the cellular antioxidant status. This imbalance would contribute to the destruction of the parasite and would be related to the cardiac damage observed in patients with chagasic cardiomyopathy.The objective of the present study was to determine the plasma activity of oxidative stress and inflammatory biomarkers: SOD, CAT, GPx, TBARS and TNF-α in chagasic patients with and without cardiomyopathy and healthy individuals.The aim of the present study is to demonstrate the predisposition to severe forms of chagasic heart disease by quantifying the biomarkers mentioned in blood from the study population.The results show significant differences in the enzymatic activities in the different groups of patients, which would mean at the cellular level, an alteration of the antioxidant capacity. Contrary to what we expected (a depletion of these enzymes), patients show an increase in antioxidant activity, that is, they respond to the generation of free radicals. The same trend is observed in the case of TBARS that are elevated in the case of chagasic patients, indicating a high degree of lipid peroxidation and oxidative damage. Regarding TNF-α levels, we found statistically significant differences, which show an active and chronic inflammatory state in these patients. Although we have found significant differences between the CN group and the other groups of patients, we should indicate that between the MCC and ECsinMCC groups, the results obtained did not show marked differences. This is important since it has been shown that patients infected with Tc have a marked antioxidant potential and are able to respond to the oxidative stress induced by the parasite, although this would not be decisive in the evolution of the disease.
{"title":"Biomarkers of Oxidative Stress and Inflammation in Chagasic Myocardiopathy","authors":"Gerrard Gabriela, Martí M. Belén, Diviani Romina, C. M. Jose, Lioi Susana, Beloscar Juan, D. Mabel","doi":"10.2174/1875318301808010017","DOIUrl":"https://doi.org/10.2174/1875318301808010017","url":null,"abstract":"The fact that only part of the population that lives in endemic areas gets Chagas disease and that only some of the patients with chronic infection develop symptoms, supports the importance of investigating the factors of each host in the susceptibility and the development of the disease. Chronic pathological processes and progressive inflammation lead to alterations in the cellular antioxidant status. This imbalance would contribute to the destruction of the parasite and would be related to the cardiac damage observed in patients with chagasic cardiomyopathy.The objective of the present study was to determine the plasma activity of oxidative stress and inflammatory biomarkers: SOD, CAT, GPx, TBARS and TNF-α in chagasic patients with and without cardiomyopathy and healthy individuals.The aim of the present study is to demonstrate the predisposition to severe forms of chagasic heart disease by quantifying the biomarkers mentioned in blood from the study population.The results show significant differences in the enzymatic activities in the different groups of patients, which would mean at the cellular level, an alteration of the antioxidant capacity. Contrary to what we expected (a depletion of these enzymes), patients show an increase in antioxidant activity, that is, they respond to the generation of free radicals. The same trend is observed in the case of TBARS that are elevated in the case of chagasic patients, indicating a high degree of lipid peroxidation and oxidative damage. Regarding TNF-α levels, we found statistically significant differences, which show an active and chronic inflammatory state in these patients. Although we have found significant differences between the CN group and the other groups of patients, we should indicate that between the MCC and ECsinMCC groups, the results obtained did not show marked differences. This is important since it has been shown that patients infected with Tc have a marked antioxidant potential and are able to respond to the oxidative stress induced by the parasite, although this would not be decisive in the evolution of the disease.","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46902990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-23DOI: 10.2174/2468422808666180523100315
Nutthapong Moonkum, Apijit Chaichana, Poladate Kantakhum, Chawanakorn Malimart, C. Piyachon, Nopawan Chananpanich, S. Mankhetkorn
{"title":"Siamois Polyphenols as Circulating Endogenous Stem Cell Regulators: Primordial Sources for Repair and Regeneration of Tissue in vivo","authors":"Nutthapong Moonkum, Apijit Chaichana, Poladate Kantakhum, Chawanakorn Malimart, C. Piyachon, Nopawan Chananpanich, S. Mankhetkorn","doi":"10.2174/2468422808666180523100315","DOIUrl":"https://doi.org/10.2174/2468422808666180523100315","url":null,"abstract":"","PeriodicalId":39398,"journal":{"name":"Open Biomarkers Journal","volume":"08 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46520241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-08DOI: 10.2174/1875318301808010009
K. Rygiel
Recent research evidence has revealed that cancer cells contain a subpopulation of cancer stem cells (CSCs) that can remain even after traditional oncology therapies (e.g.: surgical resection of a tumor, radiation therapy (RT), and chemotherapy (ChT)), and can subsequently regenerate the original tumor or metastases, which are resistant to standard anticancer treatments. Such a resistance can be activated in various CSC populations, via different signal transduction pathways.
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Pub Date : 2018-01-23DOI: 10.2174/1875318301808010001
A. Valero, M. L. Roldán, María Fernanda Ruiz, J. Teijeiro, Susana Beatriz Marquez, P. Marini
RESEARCH ARTICLE Deleted in Malignant Brain Tumor 1 (DMBT1) Expression Pattern in Normal Cervix and at Different Stages of Squamous Intraepithelial Lesions Andrés Valero, María Lorena Roldán, María Fernanda Ruiz, Juan Manuel Teijeiro, Susana Beatriz Marquez and Patricia Estela Marini Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Rosario, Argentina Servicio de Anatomía Patológica, Hospital Provincial del Centenario Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Rosario, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rosario, Argentina Consejo de Investigaciones de la Universidad Nacional de Rosario (CIUNR), Rosario, Argentina
研究Deleted in Malignant大脑肿瘤1条(DMBT1 Expression Pattern in)正常Cervix and at不同Stages of Squamous Intraepithelial Lesions安德烈,玛丽亚兄弟roldan莱罗Juan Manuel maria Fernanda Ruiz Teijeiro Patricia石碑Marini,苏珊娜Beatriz Marquez和生殖医学实验室,生物化学和制药学院,阿根廷罗萨里奥,罗萨里奥国立大学分子和细胞生物学研究所罗萨里奥(IBR-CONICET)罗萨里奥，阿根廷国家科学和技术研究委员会(CONICET)，罗萨里奥，阿根廷国家科学和技术研究委员会(CIUNR)，罗萨里奥，阿根廷
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