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Successful Management of Refractory Autoimmune Hemolytic Anemia with Cold Agglutinin Disease with Splenectomy: A Case Report with Review of Literature. 脾切除术成功治疗难治性自身免疫性溶血性贫血合并冷凝素病1例并文献复习。
Q3 Medicine Pub Date : 2023-01-11
Shuji Okamoto, Takeshi Urade, Kimikazu Yakushijin, Masahiro Kido, Kaori Kuramitsu, Shohei Komatsu, Hidetoshi Gon, Hironori Yamashita, Sachiyo Shirakawa, Daisuke Tsugawa, Sachio Terai, Hiroaki Yanagimoto, Hirochika Toyama, Takumi Fukumoto

Background: Cold agglutinin disease (CAD) is a rare autoimmune hemolytic anemia characterized by agglutination of red blood cells at temperatures below the normal core body temperature. In patients with CAD, splenectomy is not indicated because of its low therapeutic effect on hemolytic anemia induced by extravascular hemolysis. Herein, we report a case of refractory hemolytic anemia with CAD successfully managed with splenectomy.

Clinical case: A 60-year-old man visited a municipal hospital with the chief complaint of fatigue. He was found to have hemolytic anemia and icterus with increased cold agglutination and was diagnosed with CAD. Malignant lymphoma was suspected as the underlying disease; however, no clear underlying disease was identified. Hemolytic anemia progressed during the subsequent winter seasons, and he was treated with temperature control, warming, and weekly blood transfusions. However, despite the blood transfusions, his hemoglobin level did not improve during the summer 2 years after diagnosis, and his previously observed splenomegaly had progressed. He was referred to our department, and a splenectomy was performed to diagnose any occult malignant lymphoma and improve the refractory hemolytic anemia. Because histopathological examination revealed no evidence of malignant lymphoma, a diagnosis of primary CAD was made. The hemolytic anemia improved, and no blood transfusion was required after splenectomy.

Conclusions: Splenectomy significantly improved the patient's refractory hemolytic anemia due to primary CAD. Thus, it may be an effective treatment option in such cases, although further cases and studies are required to evaluate the effects of splenectomy.

背景:冷凝集素病(CAD)是一种罕见的自身免疫性溶血性贫血,其特征是红细胞在低于正常核心体温的温度下凝集。由于脾切除术对血管外溶血引起的溶血性贫血治疗效果不佳,因此不适合冠心病患者。在此,我们报告一例难治性溶血性贫血与CAD成功地处理脾切除术。临床病例:一名60岁男子以疲劳为主诉到市医院就诊。他被发现有溶血性贫血和黄疸并增加冷凝集,并被诊断为CAD。怀疑基础疾病为恶性淋巴瘤;然而,没有明确的潜在疾病被确定。溶血性贫血在随后的冬季加重,患者接受体温控制、加温和每周输血治疗。然而,尽管输血,他的血红蛋白水平在诊断后2年的夏季没有改善,他先前观察到的脾肿大有进展。他被转到我科,并进行脾切除术以诊断任何隐匿的恶性淋巴瘤和改善难治性溶血性贫血。由于组织病理学检查未发现恶性淋巴瘤的证据,因此诊断为原发性CAD。脾切除术后溶血性贫血改善,无需输血。结论:脾切除术明显改善了原发性冠心病患者的难治性溶血性贫血。因此,脾切除术可能是这种情况下的有效治疗选择,尽管需要进一步的病例和研究来评估脾切除术的效果。
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引用次数: 0
Increase in the Number and Duration of Sleep Episodes During Class After Reopening of Schools Following Closure due to COVID-19. 因COVID-19而关闭的学校重新开放后,上课期间睡眠次数和持续时间增加。
Q3 Medicine Pub Date : 2022-12-21
Shigemi Kimura, Yutaka Takaoka, Aki Sugano

Sleep is important for the well-being of school-aged children. Almost all schools in Hyogo prefecture in Japan were closed from April 7 to May 31, 2020, owing to the coronavirus disease 2019 pandemic. The pandemic restrictions resulted in the disruption of the sleep routines of children. The number of children who experienced sleepiness in class after school closure increased. The number of children who visited our hospital 1 year before and after the closure was 208 (11.73 ± 3.24 years of age) and 155 (11.45 ± 3.30 years), respectively. The number of chief complaints of sleep-related symptoms at the first visits showed no significant difference between the two time periods. The percentage of patients who slept during class increased (but not significantly) after the school closure. However, the mean number and duration of sleep episodes during class significantly increased from 0.31 ± 0.76 to 1.04 ± 1.14 episodes/day and from 15.8 ± 38.6 to 45.7 ± 46.9 min/day (each P < 0.001) before and after school closure, respectively. The total number of patients in our hospital with the primary central disorders of hypersomnolence, i.e., narcolepsy, idiopathic hypersomnia, and Kleine-Levin syndrome, and the number of patients with insufficient sleep syndrome after the school closure significantly increased compared with those before closure (P = 0.034 and 0.048, respectively). School closure was associated with an increased incidence of sleeping during class; therefore, maintaining a stable daily routine for children with sleep disorders could have an alleviating effect.

睡眠对学龄儿童的健康很重要。由于2019冠状病毒病大流行,日本兵库县几乎所有学校都于2020年4月7日至5月31日关闭。大流行病的限制导致儿童的睡眠习惯中断。放学后在课堂上犯困的孩子数量增加了。关闭前后1年来我院就诊的患儿分别为208例(11.73±3.24岁)和155例(11.45±3.30岁)。第一次就诊时主诉睡眠相关症状的数量在两个时间段之间没有显著差异。在学校关闭后,在课堂上睡觉的病人比例增加了(但并不明显)。然而,上课期间平均睡眠次数和持续时间分别从放学前的0.31±0.76次/天增加到1.04±1.14次/天,从15.8±38.6分钟增加到45.7±46.9分钟/天(P均< 0.001)。与关闭学校前相比,我校关闭学校后出现嗜睡、特发性嗜睡、Kleine-Levin综合征等嗜睡原发中枢性疾病患者总数和睡眠不足综合征患者总数均显著增加(P值分别为0.034和0.048)。学校停课与上课睡觉的发生率增加有关;因此,保持一个稳定的日常作息对睡眠障碍儿童有缓解作用。
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引用次数: 0
Longitudinal Changes and Features of Sleep Patterns of Mothers with Preterm Infants during the Early Postpartum Period. 产后早期早产儿母亲睡眠模式的纵向变化与特征。
Q3 Medicine Pub Date : 2022-12-05
Madoka Toda Miyano, Hisafumi Yasuda, Satoshi Takada

This study comprised 13 mothers of preterm infants and 21 mothers of term infants. Sleep assessment was conducted using an actigraph for three consecutive days. The participants were asked to record their sleep behaviors and activities over these 3 days, and complete two questionnaires (Edinburgh Postnatal Depression Scale [EPDS] and Pittsburgh Sleep Quality Index [PSQI]). As compared to the mothers of term infants, the sleep efficiency in the preterm mothers was significantly lower than that in the term mothers. The total sleep time was shorter and nighttime awakenings were more frequent in the preterm mothers at 2 weeks after childbirth, but without a significant difference. We analyzed the changes in the sleep data of the mothers of preterm infants longitudinally, including sleep behaviors and the EPDS and PSQI scores. The total sleep time at 1 month postpartum was shorter than that at other periods, and significantly shorter than that at 2 weeks and 6 months postpartum. Our results suggested that sleep problems tended to last longer in mothers of preterm infants than in mothers of term infants, as the problems occurred twice, immediately after childbirth and immediately after discharge.

本研究包括13名早产儿母亲和21名足月婴儿母亲。连续三天使用活动记录仪进行睡眠评估。记录3天内的睡眠行为和活动,并完成2份问卷(爱丁堡产后抑郁量表[EPDS]和匹兹堡睡眠质量指数[PSQI])。与足月母亲相比,早产母亲的睡眠效率明显低于足月母亲。早产母亲在分娩后2周的总睡眠时间较短,夜间醒来次数较多,但无显著差异。我们纵向分析了早产儿母亲睡眠数据的变化,包括睡眠行为和EPDS和PSQI评分。产后1个月总睡眠时间短于其他时期,且明显短于产后2周和6个月。我们的研究结果表明,早产婴儿的母亲的睡眠问题往往比足月婴儿的母亲持续的时间更长,因为睡眠问题会出现两次,分娩后立即出现,出院后立即出现。
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引用次数: 0
Accuracy of a Professional Continuous Glucose Monitoring Device in Individuals with Type 2 Diabetes Mellitus. 专业连续血糖监测设备在2型糖尿病患者中的准确性
Q3 Medicine Pub Date : 2022-10-03
Yasushi Nakagawa, Yushi Hirota, Akane Yamamoto, Tomofumi Takayoshi, Takehito Takeuchi, Tetsushi Hamaguchi, Atsuko Matsuoka, Kazuhiko Sakaguchi, Wataru Ogawa

Among continuous glucose monitoring (CGM) devices, which continuously measure glucose concentration in subcutaneous interstitial fluid for comprehensive monitoring of blood glucose profile, only FreeStyle Libre Pro® (Abbott Diabetes Care) is currently available in Japan as a professional system. FreeStyle Libre Pro® is easy to use because it does not require calibration by self-monitoring of blood glucose (SMBG), but information on its accuracy has been insufficient. To evaluate the measurement accuracy of FreeStyle Libre Pro®, we have now compared blood glucose levels determined by this device with those measured by SMBG in 40 individuals with type 2 diabetes mellitus. The mean absolute relative difference (MARD) for FreeStyle Libre Pro® measurements compared with SMBG measurements was calculated as an index of CGM accuracy. Overall blood glucose values measured by SMBG were 167.0 ± 60.1 mg/dL, and those determined by FreeStyle Libre Pro® were 155.0 ± 60.7 mg/dL, with this difference being statistically significant. The MARD for FreeStyle Libre Pro® relative to SMBG was 12.7 ± 9.3%. It was substantially higher in 2 of the 40 patients, at 49.2% and 47.5%, than in the other 38 individuals. MARD values did not differ significantly between before and 2 h after meals. However, the MARD was significantly higher for SMBG values of <100 mg/dL than for those of ≥250 mg/dL. Our results thus indicate that the measurement accuracy of FreeStyle Libre Pro® is relatively good, but that some cases in which values determined by the device deviate from SMBG values require caution in interpretation.

在连续血糖监测(CGM)设备中,连续测量皮下间质液中的葡萄糖浓度以全面监测血糖谱,目前只有FreeStyle Libre Pro®(雅培糖尿病护理)作为专业系统在日本上市。FreeStyle Libre Pro®易于使用,因为它不需要通过自我监测血糖(SMBG)进行校准,但有关其准确性的信息不足。为了评估FreeStyle Libre Pro®的测量准确性,我们现在将40名2型糖尿病患者的血糖水平与SMBG测量的血糖水平进行了比较。计算FreeStyle Libre Pro®测量值与SMBG测量值的平均绝对相对差(MARD),作为CGM精度的指标。SMBG测定的总血糖值为167.0±60.1 mg/dL, FreeStyle Libre Pro®测定的总血糖值为155.0±60.7 mg/dL,差异有统计学意义。FreeStyle Libre Pro®相对于SMBG的MARD为12.7±9.3%。在40名患者中,有2名患者的这一比例明显高于其他38名患者,分别为49.2%和47.5%。餐前和餐后2小时MARD值无显著差异。然而,SMBG值的MARD显著高于
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引用次数: 0
A Case with Iron Deficiency Anemia Developed Aplastic Crisis. 缺铁性贫血发生再生危机1例。
Q3 Medicine Pub Date : 2022-07-06
Kiiko Iketani, Shogo Minamikawa, Miho Sakata, Yusuke Ishida, Yasuo Nakagishi

A toddler with an unbalanced diet and gastrointestinal bleeding by juvenile polyp developed an aplastic crisis due to the human parvovirus B19 (HPVB19). Although he exhibited microcytic anemia without iron deficiency in the acute phase of HPVB19 infection, he presented with iron deficiency anemia (IDA) in the chronic phase. IDA results in erythroblast hyperplasia and shortened red blood cell lifespan as like congenital hemolytic diseases, which may lead to an aplastic crisis during HPVB19 infection. It should be noted that iron deficiency is often masked, and microcytic anemia may be a clue for IDA.

儿童饮食不均衡,消化道出血的青少年息肉发展为再生危机由于人类细小病毒B19 (HPVB19)。尽管他在HPVB19感染的急性期表现为无铁缺乏性小细胞性贫血,但在慢性期表现为缺铁性贫血(IDA)。与先天性溶血性疾病一样,IDA导致红细胞增生和红细胞寿命缩短,这可能导致HPVB19感染期间的再生危机。需要注意的是,缺铁常常被掩盖,小细胞性贫血可能是IDA的线索。
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引用次数: 0
Glycogen Storage Disease Type Ia Screening Using Dried Blood Spots on Filter Paper: Application of COP-PCR for Detection of the c.648G>T G6PC Gene Mutation. 用滤纸上的干血点筛选糖原贮积病Ia型:COP-PCR在检测c.648G>T G6PC基因突变中的应用。
Q3 Medicine Pub Date : 2021-11-02
Yogik Onky Silvana Wijaya, Emma Tabe Eko Niba, Ryo Yabushita, Yoshihiro Bouike, Hisahide Nishio, Hiroyuki Awano

Glycogen storage disease type Ia (GSDIa, OMIM #232200) is an autosomal recessive metabolic disease characterized by impaired glucose homeostasis and has a long-term complication of hepatocellular adenoma/carcinoma. GSDIa is caused by deleterious mutations in the glucose-6-phosphatase gene (G6PC). Recent studies have suggested that early treatment by gene replacement therapy may be a good solution to correct the glucose metabolism and prevent serious late complications. Early treatment of the disease needs an early disease detection system. Thus, we aimed to develop a screening system for GSDIa using dried blood spots (DBS) to detect the c.648G>T mutation in G6PC, which is a frequent mutation in the East Asian population. In this study, a total of 51 DBS samples (50 healthy controls and one patient with c.648G>T) were tested by modified competitive oligonucleotide priming PCR (mCOP-PCR). In control DBS samples, the c.648G allele was amplified at lower Cq (quantification cycle) values (<11), while the c.648T allele was amplified at higher Cq values (>14). In the patient DBS sample, the c.648T allele was amplified at a lower Cq value (<11), and the c.648G allele was amplified at a higher Cq value (>14). Based on these findings, we concluded that our mCOP-PCR system clearly differentiated the wild-type and mutant alleles, and may be applicable for screening for GSDIa with the c.648G>T mutation in G6PC.

Ia型糖原储存病(GSDIa,OMIM#223200)是一种常染色体隐性代谢性疾病,其特征是葡萄糖稳态受损,并伴有肝细胞腺瘤/癌的长期并发症。GSDIa是由葡萄糖-6-磷酸酶基因(G6PC)的有害突变引起的。最近的研究表明,通过基因替代疗法进行早期治疗可能是纠正糖代谢和预防严重晚期并发症的良好解决方案。这种疾病的早期治疗需要一个早期疾病检测系统。因此,我们旨在开发一种GSDIa筛查系统,使用干血点(DBS)来检测G6PC中的c.648G>T突变,这是东亚人群中的一种常见突变。在本研究中,共有51个DBS样本(50名健康对照和一名c.648G>T患者)通过改良竞争性寡核苷酸启动PCR(mCOP PCR)进行了检测。在对照DBS样本中,c.648G等位基因在较低的Cq(量化周期)值下扩增(14)。在患者DBS样本中,c.648T等位基因以较低的Cq值扩增(14)。基于这些发现,我们得出结论,我们的mCOP PCR系统清楚地区分了野生型和突变等位基因,并可能适用于筛查G6PC中具有c.648G>T突变的GSDIa。
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引用次数: 0
Protective Effects of Endothelin-2 Expressed in Epithelial Cells on Bleomycin-Induced Pulmonary Fibrosis in Mice. 上皮细胞中表达的内皮素-2 对博莱霉素诱导的小鼠肺纤维化的保护作用
Q3 Medicine Pub Date : 2021-11-02
Aristi Intan Soraya, Yoko Suzuki, Mitsuru Morimoto, Chemyong Jay Ko, Koji Ikeda, Ken-Ichi Hirata, Noriaki Emoto

Initially, endothelin (ET)-2 was described as an endothelium-derived vasoconstrictor. However, accumulating evidence suggests the involvement of ET-2 in non-cardiovascular physiology and disease pathophysiology. The deficiency of ET-2 in mice can be lethal, and such mice exhibit a distinct developmental abnormality in the lungs. Nonetheless, the definite role of ET-2 in the lungs remains unclear. The ET-2 isoform, ET-1, promotes pulmonary fibrosis in mice. Although endothelin receptor antagonists (ERAs) show improvements in bleomycin-induced pulmonary fibrosis in mouse models, clinical trials examining ERAs for pulmonary fibrosis treatment have been unsuccessful, even showing harmful effects in patients. We hypothesized that ET-2, which activates the same receptor as ET-1, plays a distinct role in pulmonary fibrosis. In this study, we showed that ET-2 is expressed in the lung epithelium, and ET-2 deletion in epithelial cells of mice results in the exacerbation of bleomycin-induced pulmonary fibrosis. ET-2 knockdown in lung epithelial cell lines resulted in increased apoptosis mediated via oxidative stress induction. In contrast to the effects of ET-1, which induced fibroblast activation, ET-2 hampered fibroblast activation in primary mouse lung fibroblast cells by inhibiting the TGF-β-SMAD2/3 pathway. Our results demonstrated the divergent roles of ET-1 and ET-2 in pulmonary fibrosis pathophysiology and suggested that ET-2, expressed in epithelial cells, exerts protective effects against the development of pulmonary fibrosis in mice.

最初,内皮素(ET)-2 被描述为一种源自内皮的血管收缩因子。然而,越来越多的证据表明,ET-2 参与了非心血管生理和疾病的病理生理学。小鼠缺乏 ET-2 会导致死亡,而且这种小鼠的肺部发育明显异常。然而,ET-2 在肺部的明确作用仍不清楚。ET-2 的异构体 ET-1 会促进小鼠肺纤维化。尽管内皮素受体拮抗剂(ERA)在小鼠模型中显示出对博莱霉素诱导的肺纤维化有改善作用,但将ERA用于肺纤维化治疗的临床试验却并不成功,甚至在患者身上显示出有害作用。我们假设,与 ET-1 激活相同受体的 ET-2 在肺纤维化中扮演着不同的角色。在这项研究中,我们发现 ET-2 在肺上皮细胞中表达,小鼠上皮细胞中的 ET-2 基因缺失会导致博莱霉素诱导的肺纤维化加重。在肺上皮细胞系中敲除 ET-2 会导致氧化应激诱导的细胞凋亡增加。与诱导成纤维细胞活化的 ET-1 的作用不同,ET-2 通过抑制 TGF-β-SMAD2/3 通路阻碍了原代小鼠肺成纤维细胞的活化。我们的研究结果证明了 ET-1 和 ET-2 在肺纤维化病理生理学中的不同作用,并提示在上皮细胞中表达的 ET-2 对小鼠肺纤维化的发展具有保护作用。
{"title":"Protective Effects of Endothelin-2 Expressed in Epithelial Cells on Bleomycin-Induced Pulmonary Fibrosis in Mice.","authors":"Aristi Intan Soraya, Yoko Suzuki, Mitsuru Morimoto, Chemyong Jay Ko, Koji Ikeda, Ken-Ichi Hirata, Noriaki Emoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Initially, endothelin (ET)-2 was described as an endothelium-derived vasoconstrictor. However, accumulating evidence suggests the involvement of ET-2 in non-cardiovascular physiology and disease pathophysiology. The deficiency of ET-2 in mice can be lethal, and such mice exhibit a distinct developmental abnormality in the lungs. Nonetheless, the definite role of ET-2 in the lungs remains unclear. The ET-2 isoform, ET-1, promotes pulmonary fibrosis in mice. Although endothelin receptor antagonists (ERAs) show improvements in bleomycin-induced pulmonary fibrosis in mouse models, clinical trials examining ERAs for pulmonary fibrosis treatment have been unsuccessful, even showing harmful effects in patients. We hypothesized that ET-2, which activates the same receptor as ET-1, plays a distinct role in pulmonary fibrosis. In this study, we showed that ET-2 is expressed in the lung epithelium, and ET-2 deletion in epithelial cells of mice results in the exacerbation of bleomycin-induced pulmonary fibrosis. ET-2 knockdown in lung epithelial cell lines resulted in increased apoptosis mediated via oxidative stress induction. In contrast to the effects of ET-1, which induced fibroblast activation, ET-2 hampered fibroblast activation in primary mouse lung fibroblast cells by inhibiting the TGF-β-SMAD2/3 pathway. Our results demonstrated the divergent roles of ET-1 and ET-2 in pulmonary fibrosis pathophysiology and suggested that ET-2, expressed in epithelial cells, exerts protective effects against the development of pulmonary fibrosis in mice.</p>","PeriodicalId":39560,"journal":{"name":"Kobe Journal of Medical Sciences","volume":"67 2","pages":"E61-E70"},"PeriodicalIF":0.0,"publicationDate":"2021-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622215/pdf/kobej-67-e61.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39725864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serum Cytokine Profiles of Rapid Recovery Patients with COVID-19: Series of 6 Cases. 快速康复患者6例血清细胞因子分析
Q3 Medicine Pub Date : 2021-10-25
Goh Ohji, Yohei Funakoshi, Kei Ebisawa, Kimikazu Yakushijin, Yu Arakawa, Jun Saegusa, Shinichiro Kawamoto, Takamitsu Imanishi, Yasuko Mori, Kentaro Iwata, Hironobu Minami

COVID-19 patients reveal various clinical manifestations; however, the specific mechanisms and factors contributing to rapid recovery remain unclear. We performed serum cytokine profiling using a bead-based immunoassay in six COVID-19 patients with mild symptoms who experienced rapid recovery. All patients had fever that resolved within 4 days. During the study, the interferon gamma-related protein 10 (IP-10) level rapidly increased initially, and then rapidly decreased in all six patients. Similarly, the interferon (IFN)-λ 2/3 levels rapidly increased initially, and then decreased in five of the six patients. IP-10 and IFN-λ2/3 may play a key role in the rapid recovery of mild COVID-19.

新冠肺炎患者表现出多种临床表现;然而,促进快速恢复的具体机制和因素仍不清楚。我们对6名症状轻微、恢复迅速的COVID-19患者进行了血清细胞因子分析。所有患者均在4天内退烧。在研究过程中,6例患者的干扰素γ相关蛋白10 (IP-10)水平均呈先快速升高后快速下降的趋势。同样,干扰素(IFN)-λ 2/3水平在6例患者中有5例最初迅速升高,然后下降。IP-10和IFN-λ2/3可能在轻度COVID-19快速恢复中发挥关键作用。
{"title":"Serum Cytokine Profiles of Rapid Recovery Patients with COVID-19: Series of 6 Cases.","authors":"Goh Ohji,&nbsp;Yohei Funakoshi,&nbsp;Kei Ebisawa,&nbsp;Kimikazu Yakushijin,&nbsp;Yu Arakawa,&nbsp;Jun Saegusa,&nbsp;Shinichiro Kawamoto,&nbsp;Takamitsu Imanishi,&nbsp;Yasuko Mori,&nbsp;Kentaro Iwata,&nbsp;Hironobu Minami","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>COVID-19 patients reveal various clinical manifestations; however, the specific mechanisms and factors contributing to rapid recovery remain unclear. We performed serum cytokine profiling using a bead-based immunoassay in six COVID-19 patients with mild symptoms who experienced rapid recovery. All patients had fever that resolved within 4 days. During the study, the interferon gamma-related protein 10 (IP-10) level rapidly increased initially, and then rapidly decreased in all six patients. Similarly, the interferon (IFN)-λ 2/3 levels rapidly increased initially, and then decreased in five of the six patients. IP-10 and IFN-λ2/3 may play a key role in the rapid recovery of mild COVID-19.</p>","PeriodicalId":39560,"journal":{"name":"Kobe Journal of Medical Sciences","volume":"67 2","pages":"E55-E60"},"PeriodicalIF":0.0,"publicationDate":"2021-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622216/pdf/kobej-67-e55.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39725863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural Changes in Skeletal Muscle Fibers after Icing or Heating on Downhill Running in Mice. 冰冻或加热后小鼠下坡跑步时骨骼肌纤维的结构变化。
Q3 Medicine Pub Date : 2021-09-30
Masato Kawashima, Shohei Iguchi, Naoto Fujita, Akinori Miki, Takamitsu Arakawa

An experimental animal model that causes mild structural disorders of skeletal muscles is essential to understand general exercise-induced muscle damage. Thermal stimulations such as icing and heating are commonly used as treatments for muscle injuries in sports. We established a downhill running (DR) protocol that leads to structural muscle disorders without sarcolemmal disruption and directly compared the structural changes produced by icing and heating after DR. Male ddY mice were divided into the DR, DR plus icing (Ice), and DR plus heating (Heat) groups. All mice ran at 20 m/min, -20% grade on a treadmill for a total of 90 min (three rounds of 30 min). In the Ice and Heat groups, an ice pack and a hot pack were, respectively, applied to the exercised triceps brachii muscles for 20 min just after DR. The proportion of myofibers with structural disorders was higher in the Ice group than in the DR and Heat groups at days 1 and 7 after DR. Moreover, the structural disorder of myofibers was slightly improved in the Heat group at day 1 after DR compared with the DR group. These findings suggest that icing treatment might aggravate the structural changes after DR.

一个引起骨骼肌轻度结构紊乱的实验动物模型对于理解一般运动引起的肌肉损伤是必不可少的。冷敷、加热等热刺激是治疗运动中肌肉损伤的常用方法。我们建立了在不破坏肌层的情况下导致结构性肌肉紊乱的下坡跑步(DR)方案,并直接比较了DR后冰敷和加热所产生的结构变化。雄性ddY小鼠分为DR组、DR +冰敷组(Ice)和DR +加热组(Heat)。所有小鼠在跑步机上以20米/分钟,-20%的速度跑步共90分钟(三轮30分钟)。在冰组和热组中,分别在DR后对运动的肱三头肌进行冰敷和热敷20分钟。在DR后第1天和第7天,冰组肌肉纤维结构紊乱的比例高于DR组和Heat组,并且在DR后第1天,与DR组相比,Heat组肌肉纤维结构紊乱略有改善。这些结果表明,冰敷处理可能会加剧DR后的结构变化。
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引用次数: 0
NS5A-ISGylation via Lysine 26 Has a Critical Role for Efficient Propagation of Hepatitis C Virus Genotype 2a. 赖氨酸26介导的ns5a - is酰化在基因2a型丙型肝炎病毒高效繁殖中起关键作用
Q3 Medicine Pub Date : 2021-09-30
Rheza Gandi Bawono, Takayuki Abe, Yasuaki Shibata, Chieko Matsui, Lin Deng, Ikuo Shoji

We previously reported that hepatitis C virus (HCV) NS5A (1b, Con1) protein accepts covalent ISG15 conjugation at specific lysine (Lys) residues (K44, K68, K166, K215 and K308), exhibiting proviral effects on HCV RNA replication. Here we investigated a role of NS5A-ISGylation via Lys residues in HCV propagation using HCV infectious clone. The alignment of amino acid sequences revealed that 5 Lys residues (K20, K26, K44, K139, and K166) of the 13 Lys residues within NS5A (genotype 2a, JFH1 strain) were conserved compared to those of HCV (genotype 1b, Con1 strain). The cell-based ISGylation assay revealed that the K26 residue in the amphipathic helix (AH) domain and the K139 residue in domain I of NS5A (2a, JFH1) had the potential to accept ISGylation. Use of the HCV replicon carrying luciferase gene revealed that the K26 residue but not K139 residue of NS5A (2a, JFH1) was important for HCV RNA replication. Furthermore, cell culture HCV revealed that the mutation with the K26 residue in combination with K139 or K166 on NS5A (2a, JFH1) resulted in complete abolishment of viral propagation, suggesting that the K26 residue collaborates with either the K139 residue or K166 residue for efficient HCV propagation. Taken together, these results suggest that HCV NS5A protein has the potential to accept ISGylation via specific Lys residues, involving efficient viral propagation in a genotype-specific manner.

我们之前报道了丙型肝炎病毒(HCV) NS5A (1b, Con1)蛋白在特定赖氨酸(Lys)残基(K44, K68, K166, K215和K308)上接受共价ISG15偶联,显示出对HCV RNA复制的前病毒效应。本研究利用HCV感染克隆研究了通过赖氨酸残基介导的ns5a - is酰化在HCV传播中的作用。氨基酸序列比对显示,与HCV(基因型1b, Con1株)相比,NS5A(基因型2a, JFH1株)的13个Lys残基中有5个Lys残基(K20, K26, K44, K139和K166)是保守的。基于细胞的isg酰化实验显示,NS5A (2a, JFH1)两相螺旋(AH)结构域的K26残基和结构域I的K139残基具有接受isg酰化的潜力。使用携带荧光素酶基因的HCV复制子发现,NS5A的K26残基而不是K139残基(2a, JFH1)对HCV RNA复制很重要。此外,细胞培养HCV显示,K26残基与NS5A (2a, JFH1)上的K139或K166结合的突变导致病毒的传播完全终止,这表明K26残基与K139残基或K166残基协同有效地传播HCV。综上所述,这些结果表明HCV NS5A蛋白具有通过特定的赖氨酸残基接受isg酰化的潜力,涉及以基因型特异性方式进行有效的病毒传播。
{"title":"NS5A-ISGylation via Lysine 26 Has a Critical Role for Efficient Propagation of Hepatitis C Virus Genotype 2a.","authors":"Rheza Gandi Bawono,&nbsp;Takayuki Abe,&nbsp;Yasuaki Shibata,&nbsp;Chieko Matsui,&nbsp;Lin Deng,&nbsp;Ikuo Shoji","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We previously reported that hepatitis C virus (HCV) NS5A (1b, Con1) protein accepts covalent ISG15 conjugation at specific lysine (Lys) residues (K44, K68, K166, K215 and K308), exhibiting proviral effects on HCV RNA replication. Here we investigated a role of NS5A-ISGylation via Lys residues in HCV propagation using HCV infectious clone. The alignment of amino acid sequences revealed that 5 Lys residues (K20, K26, K44, K139, and K166) of the 13 Lys residues within NS5A (genotype 2a, JFH1 strain) were conserved compared to those of HCV (genotype 1b, Con1 strain). The cell-based ISGylation assay revealed that the K26 residue in the amphipathic helix (AH) domain and the K139 residue in domain I of NS5A (2a, JFH1) had the potential to accept ISGylation. Use of the HCV replicon carrying luciferase gene revealed that the K26 residue but not K139 residue of NS5A (2a, JFH1) was important for HCV RNA replication. Furthermore, cell culture HCV revealed that the mutation with the K26 residue in combination with K139 or K166 on NS5A (2a, JFH1) resulted in complete abolishment of viral propagation, suggesting that the K26 residue collaborates with either the K139 residue or K166 residue for efficient HCV propagation. Taken together, these results suggest that HCV NS5A protein has the potential to accept ISGylation via specific Lys residues, involving efficient viral propagation in a genotype-specific manner.</p>","PeriodicalId":39560,"journal":{"name":"Kobe Journal of Medical Sciences","volume":"67 2","pages":"E38-E47"},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8622218/pdf/kobej-67-e38.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39725861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Kobe Journal of Medical Sciences
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