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Reconstruction of small diameter arteries using decellularized vascular scaffolds. 去细胞血管支架重建小直径动脉。
Q4 Dentistry Pub Date : 2014-03-19 DOI: 10.11480/610105
Yuki Nagaoka, H. Yamada, Tsuyoshi Kimura, A. Kishida, T. Fujisato, K. Takakuda
Although artificial vessels are available for large diameter arteries, there are no artificial vessels for small diameter arteries of < 4 mm. We created a decellularized vascular scaffold (length, 10 mm; outer diameter, 1.5 mm; inner diameter, 1.3 mm) from rat abdominal arteries. We measured the biomechanical characteristics of the scaffolds, implanted them to defects made in rat carotid arteries, and evaluated their patency and the endothelial cell linings. Silastic grafts were implanted as controls. The decellularized scaffolds demonstrated similar mechanical characteristics to normal arteries. All of the control grafts were occluded. Fibroblast-like cells were discovered in the thrombus, and fibrous organization was apparent. In contrast, patency of the grafts in 10 of 12 animals was observed 4 weeks after implantation. The internal cavity of the patent scaffold was completely lined by endotheliallike cells. Thus, the possibility of small artery reconstruction using decellularized scaffolds was demonstrated.
虽然对于大直径动脉有人工血管,但是对于< 4mm的小直径动脉没有人工血管。我们制作了一个脱细胞血管支架(长度,10mm;外径:1.5 mm;内径,1.3 mm)取自大鼠腹部动脉。我们测量了支架的生物力学特性,将其植入大鼠颈动脉缺损,并评估其通畅性和内皮细胞衬里。硅胶移植物作为对照。脱细胞支架表现出与正常动脉相似的力学特性。所有对照移植物均闭塞。血栓内可见成纤维细胞样细胞,纤维组织明显。相比之下,12只动物中有10只在植入后4周观察到移植物通畅。支架内腔完全被内皮样细胞衬砌。因此,证明了使用去细胞支架重建小动脉的可能性。
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引用次数: 15
Development of oral and extra-oral endosseous craniofacial implants by using a mesh structure for connective tissue attachment. 通过使用网状结构用于结缔组织附着的口腔和口腔外骨内颅面植入物的发展。
Q4 Dentistry Pub Date : 2014-03-19 DOI: 10.11480/610104
A. Mita, Atsushi Yagihara, Wei Wang, K. Takakuda
Connective tissue attachment to a mesh structure incorporated on the surface of oral implants and extra-oral endosseous craniofacial implants (EOECI) was investigated. Two types of implants were prepared: TI and TI-Mesh. TI was composed of an upper and a lower component, both comprised of a titanium cylinder, which could be connected using a titanium screw. The composition of the TIMesh was similar, but the lower cylinder had a lateral groove that was covered with a titanium mesh. In animal experiments performed using rat calvaria, the lower component was first implanted and was left submerged for 3 weeks, then the upper component was mounted percutaneously. After an additional 2 weeks, each implant and the surrounding tissues were harvested and evaluated. Histological observations revealed collagen fibers originating from surrounding hypodermal tissues anchored to the mesh structures of the TI-Mesh whereas no such collagen fibers were observed around TI. Significantly greater values of the attachment strength, the thickness of the dermal tissue, the thickness of hypodermal tissue, and the attachment lengths were observed in TI-Mesh than those of TI. Thus connective tissue attachment with collagen fibers anchored to the mesh was achieved by incorporating mesh structures into the percutaneously placed implants.
研究了口腔种植体和口腔外骨内颅面种植体(EOECI)表面结合的网状结构的结缔组织附着。制备了两种类型的种植体:TI和TI- mesh。TI由上下两部分组成,上下两部分都由一个钛筒组成,钛筒可以用钛螺钉连接。TIMesh的组成是相似的,但较低的圆柱体有一个外侧槽,覆盖着钛网。使用大鼠颅骨进行动物实验时,先植入下部构件,浸泡3周,然后经皮安装上部构件。再过2周,收集每个种植体和周围组织并评估。组织学观察显示胶原纤维起源于周围皮下组织锚定在TI- mesh的网状结构,而在TI周围没有观察到这种胶原纤维。TI- mesh的附着强度、真皮组织厚度、皮下组织厚度、附着长度均明显大于TI。因此,通过将网状结构结合到经皮放置的植入物中,可以实现与胶原纤维固定在网状物上的结缔组织附着。
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引用次数: 0
Vocal cord assessment with transnasal endoscopy in intubated patients after esophagectomy. 食管切除术后插管患者经鼻内镜声带评估。
Q4 Dentistry Pub Date : 2014-03-19 DOI: 10.11480/610103
A. Hoshino, K. Kawada, Y. Nakajima, K. Nagai, T. Sugimoto, T. Kawano
BACKGROUNDVocal cord paralysis (VCP) is one of the postoperative complications after radical esophagectomy. VCP may also lead to serious morbidities such as respiratory distress and aspiration pneumonia. Therefore, an early diagnosis of VCP is meaningful in the postoperative management of patients undergoing esophagectomy. We evaluated a new practical method for diagnosing postoperative VCP.METHODSThe laryngeal assessment of 30 patients was performed, and the presence of VCP was inferred while the following 15 patients by endoscopy before extubation after performing consecutive radical esophagectomy in 45 esophageal cancer patients. The vocal cord mobility, including adduction and abduction, were assessed by inserting the tip of a transnasal endoscope near the vocal cord in the awake patients with orotracheal intubation on the first postoperative day. The presence of VCP was reevaluated after extubation.RESULTSEleven of the 30 patients assessed after radical esophagectomy had unilateral VCP and one patient had bilateral VCP. The abduction findings were useful for assessing VCP and the sensitivity, specificity, positive and negative predictive values and accuracy were 97.9%, 100%, 100%, 92.9% and 98.3%, respectively. The results of the following 15 patients were closely similar.CONCLUSIONSThe VCP during orotracheal intubation is assessable by transnasal endoscopy.
背景:声带麻痹(VCP)是根治性食管切除术后的并发症之一。VCP还可能导致严重的疾病,如呼吸窘迫和吸入性肺炎。因此,早期诊断VCP对食管切除术患者的术后处理具有重要意义。我们评估了一种新的实用的诊断术后VCP的方法。方法对45例食管癌患者进行连续根治性食管切除术后拔管前内镜检查的15例患者,对30例患者进行喉部检查,推断是否存在VCP。术后第一天,清醒的经气管插管患者将经鼻内窥镜尖端插入声带附近,评估声带内收和外展的活动情况。拔管后重新评估VCP的存在。结果30例根治性食管切除术患者中有7例发生单侧VCP, 1例发生双侧VCP。外展检查结果可用于评估VCP,其敏感性、特异性、阳性预测值和阴性预测值分别为97.9%、100%、100%、92.9%和98.3%。以下15例患者的结果非常相似。结论经鼻内镜可评估气管插管时的VCP。
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引用次数: 1
Effectiveness of setting numerical targets in the surgical training of residents: a trial to achieve an optimal balance. 在住院医师外科培训中设定数值目标的有效性:达到最佳平衡的试验。
Q4 Dentistry Pub Date : 2014-01-17
Kiyoshi Komiya, Momoko Saito, Yuika Sakurai, Hiromi Kojima, Kozo Takase

During the past 10 years, residency training in otorhinolaryngology-head and neck surgery (ORL-HNS) in Japan, especially at university hospitals, has emphasized subspecialization, resulting in insufficiencies in basic surgical techniques with an extreme bias toward acquiring subspecialty surgical case experience. To address this problem, we developed a target-oriented program intended to achieve a more balanced approach to surgical training and performed a 1-year trial of the program at the Jikei University School of Medicine. Fourteen residents with 1 to 4 years of ORL-HNS experience completed the trial. Each resident's competencies in six basic surgical procedures were assessed on the basis of the number of cases handled by the resident, and each resident's case selection bias after implementation of the target-oriented training was examined. The case selection bias in the trial group residents was reduced and their balance in case experience was shown to be improved in comparison with that in control group residents who were trained in the conventional way. In addition, opinion surveys of the participants and supervising otorhinolaryngologists (trainers) indicated that they felt that the new training system had been effective in improving the balance in case experience and improving motivation, and creating greater awareness of training goals and progress.

在过去的10年里,日本的耳鼻喉头颈外科(ORL-HNS)住院医师培训,特别是在大学医院,强调亚专科,导致基本手术技术的不足,极端倾向于获得亚专科手术病例经验。为了解决这个问题,我们制定了一个目标导向的计划,旨在实现更平衡的外科培训方法,并在智庆大学医学院进行了为期一年的试验。14位具有1至4年ORL-HNS经验的住院医师完成了试验。根据住院医师处理的病例数量评估每位住院医师在六种基本外科手术方面的能力,并检查每位住院医师在实施目标导向培训后的病例选择偏差。与以传统方式训练的对照组居民相比,试验组居民的病例选择偏差减少,他们在病例经验中的平衡得到了改善。此外,对学员和督导耳鼻喉科医生(培训师)的意见调查显示,他们认为新的培训制度在改善病例经验平衡、提高积极性、提高对培训目标和进展的认识方面是有效的。
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引用次数: 0
Changes in the microvascular structure of mucosal squamous cell carcinoma of the esophagus and their significance in tumor progression. 食管粘膜鳞状细胞癌微血管结构的变化及其在肿瘤进展中的意义。
Q4 Dentistry Pub Date : 2014-01-17
Jirawat Swangsri, Yasuaki Nakajima, Kenro Kawada, Yutaka Tokairin, Tomoyoshi Suzuki, Yutaka Miyawaki, Akihiro Hoshino, Takuya Okada, Shunsuke Ota, Tairo Ryotokuji, Naoto Fujiwara, Tetsuro Nishikage, Kagami Nagai, Hiroshi Kawachi, Tatsuyuki Kawano

Background: To identify the clinical T stage by endoscopy is a major diagnostic goal for superficial esophageal squamous cell carcinoma (ESCC). The completion of a microvascular morphological study of mucosal lesions is necessary to optimize therapy.

Materials and methods: Images of 197 intra-papillary capillary loops (IPCLs) captured by magnified endoscopy from 15 esophagectomy specimens were studied for their morphological features and IPCL dimensions.

Results: The microvascular morphology was classified into four basic major patterns: 1. spiral loop, 2. wide loop (WL), 3. globular (G) and 4. reticular pattern. The microvascular features and dimensions differed according to the depth of tumor invasion. Especially the mean bundle outline (IPCL diameter) showed significant changes as 20.02, 22.32, and 27.08 μm, respectively, for M1, M2 and M3, respectively (M1:M2 P < 0.05, M2:M3 P < 0.01).

Conclusions: During tumor stage progression, a high-volume blood demand and cancer cell overgrowth to occupy the laminar propria mucosa (LPM) cause obvious elongation, thickening, branching, irregularity and deformity of the IPCL, which were characteristics of M3 lesions. The results of the present study support and can be applied with the current Japanese classification for improving the diagnostic accuracy, especially to differentiate between M2 and M3 lesions based on the endoscopic findings.

背景:内镜下确定临床T分期是浅表性食管鳞状细胞癌(ESCC)的主要诊断目标。完成粘膜病变的微血管形态学研究对于优化治疗是必要的。材料与方法:对15例食管切除术标本中197个乳头内毛细血管袢(IPCL)的放大内镜图像进行形态学特征和IPCL尺寸的研究。结果:微血管形态可分为四种基本形态:1。螺旋环,2。宽回路(WL), 3;球状(G)和4。网状模式。肿瘤浸润深度不同,微血管形态及大小也不同。其中,M1、M2和M3的平均束廓(IPCL直径)变化显著,分别为20.02、22.32和27.08 μm (M1:M2 P < 0.05, M2:M3 P < 0.01)。结论:在肿瘤进展阶段,大量血需求和癌细胞过度生长占据固有层黏膜(LPM),导致IPCL明显伸长、增厚、分支、不规则和畸形,这是M3病变的特征。本研究结果支持并可应用于目前的日本分类,以提高诊断准确性,特别是根据内镜检查结果区分M2和M3病变。
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引用次数: 0
Effectiveness of setting numerical targets in the surgical training of residents: a trial to achieve an optimal balance. 在住院医师外科培训中设定数值目标的有效性:达到最佳平衡的试验。
Q4 Dentistry Pub Date : 2013-12-01 DOI: 10.11480/JMDS.600402
K. Komiya, Momoko Saito, Yuika Sakurai, H. Kojima, K. Takase
During the past 10 years, residency training in otorhinolaryngology-head and neck surgery (ORL-HNS) in Japan, especially at university hospitals, has emphasized subspecialization, resulting in insufficiencies in basic surgical techniques with an extreme bias toward acquiring subspecialty surgical case experience. To address this problem, we developed a target-oriented program intended to achieve a more balanced approach to surgical training and performed a 1-year trial of the program at the Jikei University School of Medicine. Fourteen residents with 1 to 4 years of ORL-HNS experience completed the trial. Each resident's competencies in six basic surgical procedures were assessed on the basis of the number of cases handled by the resident, and each resident's case selection bias after implementation of the target-oriented training was examined. The case selection bias in the trial group residents was reduced and their balance in case experience was shown to be improved in comparison with that in control group residents who were trained in the conventional way. In addition, opinion surveys of the participants and supervising otorhinolaryngologists (trainers) indicated that they felt that the new training system had been effective in improving the balance in case experience and improving motivation, and creating greater awareness of training goals and progress.
在过去的10年里,日本的耳鼻喉头颈外科(ORL-HNS)住院医师培训,特别是在大学医院,强调亚专科,导致基本手术技术的不足,极端倾向于获得亚专科手术病例经验。为了解决这个问题,我们制定了一个目标导向的计划,旨在实现更平衡的外科培训方法,并在智庆大学医学院进行了为期一年的试验。14位具有1至4年ORL-HNS经验的住院医师完成了试验。根据住院医师处理的病例数量评估每位住院医师在六种基本外科手术方面的能力,并检查每位住院医师在实施目标导向培训后的病例选择偏差。与以传统方式训练的对照组居民相比,试验组居民的病例选择偏差减少,他们在病例经验中的平衡得到了改善。此外,对学员和督导耳鼻喉科医生(培训师)的意见调查显示,他们认为新的培训制度在改善病例经验平衡、提高积极性、提高对培训目标和进展的认识方面是有效的。
{"title":"Effectiveness of setting numerical targets in the surgical training of residents: a trial to achieve an optimal balance.","authors":"K. Komiya, Momoko Saito, Yuika Sakurai, H. Kojima, K. Takase","doi":"10.11480/JMDS.600402","DOIUrl":"https://doi.org/10.11480/JMDS.600402","url":null,"abstract":"During the past 10 years, residency training in otorhinolaryngology-head and neck surgery (ORL-HNS) in Japan, especially at university hospitals, has emphasized subspecialization, resulting in insufficiencies in basic surgical techniques with an extreme bias toward acquiring subspecialty surgical case experience. To address this problem, we developed a target-oriented program intended to achieve a more balanced approach to surgical training and performed a 1-year trial of the program at the Jikei University School of Medicine. Fourteen residents with 1 to 4 years of ORL-HNS experience completed the trial. Each resident's competencies in six basic surgical procedures were assessed on the basis of the number of cases handled by the resident, and each resident's case selection bias after implementation of the target-oriented training was examined. The case selection bias in the trial group residents was reduced and their balance in case experience was shown to be improved in comparison with that in control group residents who were trained in the conventional way. In addition, opinion surveys of the participants and supervising otorhinolaryngologists (trainers) indicated that they felt that the new training system had been effective in improving the balance in case experience and improving motivation, and creating greater awareness of training goals and progress.","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 4 1","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64011801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Changes in the microvascular structure of mucosal squamous cell carcinoma of the esophagus and their significance in tumor progression. 食管粘膜鳞状细胞癌微血管结构的变化及其在肿瘤进展中的意义。
Q4 Dentistry Pub Date : 2013-12-01 DOI: 10.11480/JMDS.600401
Jirawat Swangsri, Y. Nakajima, K. Kawada, Y. Tokairin, Tomoyoshi Suzuki, Y. Miyawaki, A. Hoshino, T. Okada, S. Ota, Tairo Ryotokuji, N. Fujiwara, T. Nishikage, K. Nagai, H. Kawachi, T. Kawano
BACKGROUNDTo identify the clinical T stage by endoscopy is a major diagnostic goal for superficial esophageal squamous cell carcinoma (ESCC). The completion of a microvascular morphological study of mucosal lesions is necessary to optimize therapy.MATERIALS AND METHODSImages of 197 intra-papillary capillary loops (IPCLs) captured by magnified endoscopy from 15 esophagectomy specimens were studied for their morphological features and IPCL dimensions.RESULTSThe microvascular morphology was classified into four basic major patterns: 1. spiral loop, 2. wide loop (WL), 3. globular (G) and 4. reticular pattern. The microvascular features and dimensions differed according to the depth of tumor invasion. Especially the mean bundle outline (IPCL diameter) showed significant changes as 20.02, 22.32, and 27.08 μm, respectively, for M1, M2 and M3, respectively (M1:M2 P < 0.05, M2:M3 P < 0.01).CONCLUSIONSDuring tumor stage progression, a high-volume blood demand and cancer cell overgrowth to occupy the laminar propria mucosa (LPM) cause obvious elongation, thickening, branching, irregularity and deformity of the IPCL, which were characteristics of M3 lesions. The results of the present study support and can be applied with the current Japanese classification for improving the diagnostic accuracy, especially to differentiate between M2 and M3 lesions based on the endoscopic findings.
背景内镜鉴别临床T分期是浅表性食管鳞状细胞癌(ESCC)的主要诊断目标。完成粘膜病变的微血管形态学研究对于优化治疗是必要的。材料与方法利用放大内镜对15例食管切除术标本中197个乳头内毛细血管袢(IPCL)的形态学特征和IPCL的尺寸进行研究。结果微血管形态可分为四种基本形态:1.血管形态;螺旋环,2。宽回路(WL), 3;球状(G)和4。网状模式。肿瘤浸润深度不同,微血管形态及大小也不同。其中,M1、M2和M3的平均束廓(IPCL直径)变化显著,分别为20.02、22.32和27.08 μm (M1:M2 P < 0.05, M2:M3 P < 0.01)。结论在肿瘤进展阶段,大量血需求和癌细胞过度生长侵占固有层黏膜(LPM),导致IPCL明显伸长、增厚、分支、不规则和畸形,这是M3病变的特征。本研究结果支持并可应用于目前的日本分类,以提高诊断准确性,特别是根据内镜检查结果区分M2和M3病变。
{"title":"Changes in the microvascular structure of mucosal squamous cell carcinoma of the esophagus and their significance in tumor progression.","authors":"Jirawat Swangsri, Y. Nakajima, K. Kawada, Y. Tokairin, Tomoyoshi Suzuki, Y. Miyawaki, A. Hoshino, T. Okada, S. Ota, Tairo Ryotokuji, N. Fujiwara, T. Nishikage, K. Nagai, H. Kawachi, T. Kawano","doi":"10.11480/JMDS.600401","DOIUrl":"https://doi.org/10.11480/JMDS.600401","url":null,"abstract":"BACKGROUND\u0000To identify the clinical T stage by endoscopy is a major diagnostic goal for superficial esophageal squamous cell carcinoma (ESCC). The completion of a microvascular morphological study of mucosal lesions is necessary to optimize therapy.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Images of 197 intra-papillary capillary loops (IPCLs) captured by magnified endoscopy from 15 esophagectomy specimens were studied for their morphological features and IPCL dimensions.\u0000\u0000\u0000RESULTS\u0000The microvascular morphology was classified into four basic major patterns: 1. spiral loop, 2. wide loop (WL), 3. globular (G) and 4. reticular pattern. The microvascular features and dimensions differed according to the depth of tumor invasion. Especially the mean bundle outline (IPCL diameter) showed significant changes as 20.02, 22.32, and 27.08 μm, respectively, for M1, M2 and M3, respectively (M1:M2 P < 0.05, M2:M3 P < 0.01).\u0000\u0000\u0000CONCLUSIONS\u0000During tumor stage progression, a high-volume blood demand and cancer cell overgrowth to occupy the laminar propria mucosa (LPM) cause obvious elongation, thickening, branching, irregularity and deformity of the IPCL, which were characteristics of M3 lesions. The results of the present study support and can be applied with the current Japanese classification for improving the diagnostic accuracy, especially to differentiate between M2 and M3 lesions based on the endoscopic findings.","PeriodicalId":39643,"journal":{"name":"Journal of Medical and Dental Sciences","volume":"60 4 1","pages":"83-91"},"PeriodicalIF":0.0,"publicationDate":"2013-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64011789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Evaluation of the occlusal contact of crowns fabricated with the bite impression method. 牙合印模法制备冠的咬合接触评价。
Q4 Dentistry Pub Date : 2013-09-30
Sachi Makino, Daizo Okada, Chiharu Shin, Reiko Ogura, Masaomi Ikeda, Hiroyuki Miura

In prosthodontic treatment, reconstruction of a proper occlusal contact relationship is very important as well as reconstruction of a proper interproximal relationship and marginal fitness. Unfortunately, occlusal relationships are sometimes lost in the process of occlusal adjustment of crowns. The purpose of this study was to compare the occlusal contacts of single crown fabricated by two different types of impression techniques. Nine subjects, whose molars required treatment with crown restoration, were enrolled in this study. Full cast crowns were fabricated using two types of impression techniques: the conventional impression method (CIM) and the bite impression method (BIM). The occlusal contacts of crowns were precisely evaluated at the following stages: after occlusal adjustment on the articulator (Step 0), before occlusal adjustment in the mouth (Step 1), after occlusal adjustment at the intercuspal position (Step 2), and after occlusal adjustment during lateral and protrusive excursions (Step 3). The number of occlusal contacts of the crowns on the functional cusps fabricated with BIM was significantly greater than that with CIM after occlusal adjustment. For this reason, the crowns fabricated with BIM might have a more functionally desirable occlusal surface compared to the crowns fabricated with CIM.

在修复治疗中,重建正确的咬合接触关系以及重建正确的近端间关系和边缘适应度是非常重要的。不幸的是,在冠的咬合调整过程中,有时会丢失咬合关系。本研究的目的是比较两种不同印模技术制作的单冠的咬合接触。本研究纳入了9名臼齿需要冠修复治疗的受试者。全铸冠的制作采用两种压模技术:常规压模法(CIM)和咬合压模法(BIM)。在咬合调整后(步骤0)、口腔内咬合调整前(步骤1)、牙尖间位置咬合调整后(步骤2)、侧突运动咬合调整后(步骤3)对冠的咬合接触进行精确评估。使用BIM制作的冠在功能牙尖上的咬合接触数量明显大于使用CIM制作的冠在咬合调整后的咬合接触数量。因此,与使用CIM制造的冠相比,使用BIM制造的冠可能具有更理想的咬合面。
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引用次数: 0
Efficacy of lidocaine lontophoresis using either alternating or direct current in hairless rats. 交流电或直流电利多卡因对无毛大鼠lontopresis的影响。
Q4 Dentistry Pub Date : 2013-09-30 DOI: 10.11480/JMDS.600301
A. Nakajima, R. Wakita, H. Haida, H. Fukayama
The aim of this study was to determine transport of lidocaine ions through a hairless rat skin in vivo and to compare the efficacy of alternating current (AC) with that of direct current (DC) iontophoresis (IOP). We measured the concentration of lidocaine transported through a cellophane membrane or a hairless rat dorsal skin applying either AC-IOP or DC-IOP. The results revealed that lidocaine concentration increased in a time-dependent manner in vitro in both DC-IOP and AC-IOP. However, the in vivo study showed different tendencies in lidocaine concentration. In the DCIOP group, lidocaine concentration reached its maximum 20 min after current application and then decreased rapidly; the AC-IOP group showed an increase in lidocaine concentration in a time-dependent manner. There were no side effects such as electrical burns in the rats. In conclusion, AC can be applied for long periods and DC for short periods, or their application time can be appropriately scheduled. Our study also suggests the mechanism by which voltage waveforms affect the skin when applied by IOP. In the future, these findings will be a solid foundation for developing various kinds of medical equipment such as scheduled drug delivery system that can easily deliver various types of drug.
本研究的目的是测定利多卡因离子在体内无毛大鼠皮肤中的转运,并比较交流电(AC)和直流电(DC)离子透入(IOP)的效果。我们测量了利多卡因通过玻璃纸膜或无毛大鼠背部皮肤施加AC-IOP或DC-IOP的浓度。结果显示,体外DC-IOP和AC-IOP的利多卡因浓度均呈时间依赖性升高。然而,体内研究显示利多卡因浓度有不同的趋势。在DCIOP组,利多卡因浓度在给药后20分钟达到最大值,然后迅速下降;AC-IOP组利多卡因浓度呈时间依赖性增加。在大鼠身上没有电烧伤等副作用。综上所述,交流电可以长期使用,直流电可以短期使用,或者适当安排其使用时间。我们的研究也提示了当施加IOP时电压波形影响皮肤的机制。在未来,这些发现将成为开发各种医疗设备的坚实基础,例如可以轻松输送各种类型药物的定时给药系统。
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引用次数: 3
Evaluation of the occlusal contact of crowns fabricated with the bite impression method. 牙合印模法制备冠的咬合接触评价。
Q4 Dentistry Pub Date : 2013-09-30 DOI: 10.11480/JMDS.600302
Sachi Makino, D. Okada, C. Shin, R. Ogura, M. Ikeda, H. Miura
In prosthodontic treatment, reconstruction of a proper occlusal contact relationship is very important as well as reconstruction of a proper interproximal relationship and marginal fitness. Unfortunately, occlusal relationships are sometimes lost in the process of occlusal adjustment of crowns. The purpose of this study was to compare the occlusal contacts of single crown fabricated by two different types of impression techniques. Nine subjects, whose molars required treatment with crown restoration, were enrolled in this study. Full cast crowns were fabricated using two types of impression techniques: the conventional impression method (CIM) and the bite impression method (BIM). The occlusal contacts of crowns were precisely evaluated at the following stages: after occlusal adjustment on the articulator (Step 0), before occlusal adjustment in the mouth (Step 1), after occlusal adjustment at the intercuspal position (Step 2), and after occlusal adjustment during lateral and protrusive excursions (Step 3). The number of occlusal contacts of the crowns on the functional cusps fabricated with BIM was significantly greater than that with CIM after occlusal adjustment. For this reason, the crowns fabricated with BIM might have a more functionally desirable occlusal surface compared to the crowns fabricated with CIM.
在修复治疗中,重建正确的咬合接触关系以及重建正确的近端间关系和边缘适应度是非常重要的。不幸的是,在冠的咬合调整过程中,有时会丢失咬合关系。本研究的目的是比较两种不同印模技术制作的单冠的咬合接触。本研究纳入了9名臼齿需要冠修复治疗的受试者。全铸冠的制作采用两种压模技术:常规压模法(CIM)和咬合压模法(BIM)。在咬合调整后(步骤0)、口腔内咬合调整前(步骤1)、牙尖间位置咬合调整后(步骤2)、侧突运动咬合调整后(步骤3)对冠的咬合接触进行精确评估。使用BIM制作的冠在功能牙尖上的咬合接触数量明显大于使用CIM制作的冠在咬合调整后的咬合接触数量。因此,与使用CIM制造的冠相比,使用BIM制造的冠可能具有更理想的咬合面。
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引用次数: 1
期刊
Journal of Medical and Dental Sciences
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