Soursop leaf (Annona muricata L.) is an herbal plant that is widely used by the public empirically because it has many properties. The sling leaf contains tannin compounds, alkaloids, as well as several other chemical compounds including annonaceous acetogenin, which can be used as a treatment. This study aims to find out if soursop leaf extract has the effect of deducting blood glucose levels in mice test animals (Mus musculus) which induced a 50% glucose solution. The study used the RAL method (complete random design) and 20 male mice were divided into 5 groups with 4 repetitions. Group P1, P2, and P3 (treatment group) administered ethanol extract of sine leaves at doses of 250, 500, and 750 mg/kg bb respectively, while k- and k+ (control group) administered CMC 0.5% and glibenclamide with oral administration. The data obtained were statistically analyzed with ANOVA (Variance analysis) one-way to find out the differences between treatment groups and continued with the BNT test (The smallest real difference) to find out meaningful differences between groups. The results showed that there were differences between the treatment group and the control group. On the 7th day of the BNT test, the treatment group with a dose of 750 mg/kg bb showed significant differences between the negative Control group (CMC 0'5%), Positive control (Glibenclamide), P1 (250 mg/kg bb), and P2 (500 mg/kg bb), while on the 14th day showed no significant difference with the treatment group of 500 mg/kg bb. concluded from this study that all concentrations of ethanol extract of sling leaves could lower blood glucose levels in test animals, with the most optimal decrease being the group at a dose of 750 mg/kg bb.
{"title":"Antihyperglycemic Test of Ethanol Extract of Soursop Leaf (Annona muricata L.) on Blood Sugar Levels of Male Mouse (Mus musculus) Induced by Glucose","authors":"Gusti putu Eka Kusuma","doi":"10.31258/jnat.19.1.1-5","DOIUrl":"https://doi.org/10.31258/jnat.19.1.1-5","url":null,"abstract":"Soursop leaf (Annona muricata L.) is an herbal plant that is widely used by the public empirically because it has many properties. The sling leaf contains tannin compounds, alkaloids, as well as several other chemical compounds including annonaceous acetogenin, which can be used as a treatment. This study aims to find out if soursop leaf extract has the effect of deducting blood glucose levels in mice test animals (Mus musculus) which induced a 50% glucose solution. The study used the RAL method (complete random design) and 20 male mice were divided into 5 groups with 4 repetitions. Group P1, P2, and P3 (treatment group) administered ethanol extract of sine leaves at doses of 250, 500, and 750 mg/kg bb respectively, while k- and k+ (control group) administered CMC 0.5% and glibenclamide with oral administration. The data obtained were statistically analyzed with ANOVA (Variance analysis) one-way to find out the differences between treatment groups and continued with the BNT test (The smallest real difference) to find out meaningful differences between groups. The results showed that there were differences between the treatment group and the control group. On the 7th day of the BNT test, the treatment group with a dose of 750 mg/kg bb showed significant differences between the negative Control group (CMC 0'5%), Positive control (Glibenclamide), P1 (250 mg/kg bb), and P2 (500 mg/kg bb), while on the 14th day showed no significant difference with the treatment group of 500 mg/kg bb. concluded from this study that all concentrations of ethanol extract of sling leaves could lower blood glucose levels in test animals, with the most optimal decrease being the group at a dose of 750 mg/kg bb.","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131077539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-09DOI: 10.31258/jnat.19.1.23-28
Muhammad Zebi Fernando, E. Efriyeldi, A. Mulyadi
Morphometrics is a field that deals with variations and changes in the form (size and shape) of an organism or object. Anthropogenic is a human activity not only limited to the use or utilization of coastal resources, but also can be restorative and protective, thus causing complex disruption. The purpose of this study was to determine the morphometric differences of the flowers and fruits of R.apiculata mangroves in two regions with different anthropogenic activities in the mangrove ecosystem of Dumai City. This research was carried out in September 2019. From the results of the study it was found that in areas polluted by anthropogenic activity the average morphometrics of fruit (length and width) and leaves (length, width and length of the stems) Rhizophora apiculata were lower than those in the area not polluted by anthropogenic activity. At station I the oil content ranged from 0,0975 to 0,1251 ppm classified as polluted and at station II ranged from 0,072 to 0,111 ppm classified as not polluted.
{"title":"Comparative Morphometric Rhizophora ApiculataFlower and Fruit in Two Areas with Anthropogenic Activities in West Dumai Coastal, Dumai City, Riau Province","authors":"Muhammad Zebi Fernando, E. Efriyeldi, A. Mulyadi","doi":"10.31258/jnat.19.1.23-28","DOIUrl":"https://doi.org/10.31258/jnat.19.1.23-28","url":null,"abstract":"Morphometrics is a field that deals with variations and changes in the form (size and shape) of an organism or object. Anthropogenic is a human activity not only limited to the use or utilization of coastal resources, but also can be restorative and protective, thus causing complex disruption. The purpose of this study was to determine the morphometric differences of the flowers and fruits of R.apiculata mangroves in two regions with different anthropogenic activities in the mangrove ecosystem of Dumai City. This research was carried out in September 2019. From the results of the study it was found that in areas polluted by anthropogenic activity the average morphometrics of fruit (length and width) and leaves (length, width and length of the stems) Rhizophora apiculata were lower than those in the area not polluted by anthropogenic activity. At station I the oil content ranged from 0,0975 to 0,1251 ppm classified as polluted and at station II ranged from 0,072 to 0,111 ppm classified as not polluted.","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121642379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-09DOI: 10.31258/jnat.19.1.18-22
Siti Malikha
Bekasam is a type of fermented freshwater fish product with sour taste. Bekasam is made from fish that have been cleaned gills and entrails, washed, mixed with salt. After 2 days of fermentation, rice is added to this mixture and fermentation is continued for 5 to 7 days. Bekasam is thought to have antihypertensive activity due to the formation of bioactive peptides resulting from fish protein degradation during the fermentation process of the scars. This study aims to isolate indigenous lactic acid bacteria with high power. Lactic acid bacteria were isolated from tuna. Isolation was carried out using MRS agar + CaCO3 medium and screening of proteolytic lactic acid bacterial isolates was carried out using skim agar. Colonies that provide a clear zone on the skim to be identified as lactic acid bacteria. Of the 180 isolates of acid-producing bacteria that provide clear zones in the MRS to find 150 strains of lactic acid bacteria. The results of further morphological and biochemical tests indicate that these isolates are genera Lactobacillus, Pediococccus and Leuconostoc. The results of further selection were carried out on homofermentative lactic acid bacteria and had high proteolytic activity. The results of identification of isolate species using API CH50 showed that selected lactic acid bacteria were Lactobacillus plantarum, Lactobacillus pentosus, and Pediococcus pentosaceus
{"title":"Review of the Characterization of Lactic Acid Bacteria in Tuna Fish","authors":"Siti Malikha","doi":"10.31258/jnat.19.1.18-22","DOIUrl":"https://doi.org/10.31258/jnat.19.1.18-22","url":null,"abstract":"Bekasam is a type of fermented freshwater fish product with sour taste. Bekasam is made from fish that have been cleaned gills and entrails, washed, mixed with salt. After 2 days of fermentation, rice is added to this mixture and fermentation is continued for 5 to 7 days. Bekasam is thought to have antihypertensive activity due to the formation of bioactive peptides resulting from fish protein degradation during the fermentation process of the scars. This study aims to isolate indigenous lactic acid bacteria with high power. Lactic acid bacteria were isolated from tuna. Isolation was carried out using MRS agar + CaCO3 medium and screening of proteolytic lactic acid bacterial isolates was carried out using skim agar. Colonies that provide a clear zone on the skim to be identified as lactic acid bacteria. Of the 180 isolates of acid-producing bacteria that provide clear zones in the MRS to find 150 strains of lactic acid bacteria. The results of further morphological and biochemical tests indicate that these isolates are genera Lactobacillus, Pediococccus and Leuconostoc. The results of further selection were carried out on homofermentative lactic acid bacteria and had high proteolytic activity. The results of identification of isolate species using API CH50 showed that selected lactic acid bacteria were Lactobacillus plantarum, Lactobacillus pentosus, and Pediococcus pentosaceus","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127506875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-04-09DOI: 10.31258/jnat.19.1.13-17
R. Kurniawan
Aquaculture production is vulnerable to adverse impacts to disease and environmental conditions. One of the pathogenic bacteria that infection cultured is Edwardsiella tarda. The aim of this study was to find out the sensitivity of R.apiculata leaf extract in inhibiting the growth of E.tarda. The research method used is the experimental method, with the Kirby-Bauer disc method. The doses used were 100% (10000 ppm), 90% (9000 ppm), 80% (8000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline), blank discs used were 6 mm in size. The results showed that the ethanolic extract of the leaves of R. apiculata at a dose of 1000-10000 ppm gave various inhibitory diameters ranging from 6.27-9.87 mm and presenting inhibition zone diameters in the medium category
{"title":"Antibacterial activity of Rhizophora apiculata leaf extract against Edwardsiella tarda bacteria","authors":"R. Kurniawan","doi":"10.31258/jnat.19.1.13-17","DOIUrl":"https://doi.org/10.31258/jnat.19.1.13-17","url":null,"abstract":"Aquaculture production is vulnerable to adverse impacts to disease and environmental conditions. One of the pathogenic bacteria that infection cultured is Edwardsiella tarda. The aim of this study was to find out the sensitivity of R.apiculata leaf extract in inhibiting the growth of E.tarda. The research method used is the experimental method, with the Kirby-Bauer disc method. The doses used were 100% (10000 ppm), 90% (9000 ppm), 80% (8000 ppm), 70% (7000 ppm), 60% (6000 ppm), 50% (5000 ppm), 40% (4000 ppm), 30% (3000 ppm), 20% (2000 ppm), 10% (1000 ppm) and control (Oxytetracycline), blank discs used were 6 mm in size. The results showed that the ethanolic extract of the leaves of R. apiculata at a dose of 1000-10000 ppm gave various inhibitory diameters ranging from 6.27-9.87 mm and presenting inhibition zone diameters in the medium category","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"239 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122984551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Hasan Basri Nasution, S. Ramadhani, E. Fachrial
Lactic Acid Bacteria (LAB) are gram-positive bacteria that are rod or round in shape, do not form spores, and do not have cytochromes. LAB can be isolated from fermented food products. LAB is useful to inhibit the growth of pathogenic bacteria so that they are potential antibacterial sources. The purpose of this study was to isolate LAB from the traditional food Dali Ni Horbo, continued with characterization, antimicrobial testing, and sensitivity testing towards several antibiotics. From the results of the study, the number of colonies obtained was 2.4 x 107 CFU/mL. 6 isolates were randomly selected, coded as isolates UPDH1, UPDH2, UPDH3, UPDH4, UPDH5, UPDH6, respectively. Biochemical characterization showed that the isolates were gram-positive and catalase-negative bacteria. Results of antibiotic sensitivity testing using 6 antibiotics, namely amoxicillin (AML), erythromycin (E), oxacillin (OX), ofloxacin (OFX), cefotaxime (CTX), and gentamicin (CN), showed that all the isolates were resistant to CTX. The UPDH1 isolate was resistant to AML and OX. The UPDH3, UPDH5, and UPDH6 isolates were resistant to AML, and the UPDH4 isolate was resistant to E, OFX, CTX, CN, and OX. Antimicrobial tests used the disk diffusion method against Escherichia coli and Staphylococcus aureus. The UPDH2 isolate, gave an inhibition zone of 6.7 mm, and UPDH5 and UPDH6 gave inhibition zones of 7.6 mm and 8.5 mm respectively, against E. coli. Microbial inhibition tests against S. aureus by UPDH1, UPDH2, UPDH5 and UPDH6 gave inhibition zones of 13.5 mm, 9.0 mm, 12.1 mm and 12 mm respectively. From the results in this research it can be concluded that the traditional food Dali Ni Horbo is a potential source of probiotics.
{"title":"Isolation, Characterization and Antibacterial Activities of Lactic Acid Bacteria Isolated From Batak's Special Food \"Dali Ni Horbo”","authors":"Muhammad Hasan Basri Nasution, S. Ramadhani, E. Fachrial","doi":"10.31258/jnat.18.1.1-11","DOIUrl":"https://doi.org/10.31258/jnat.18.1.1-11","url":null,"abstract":"Lactic Acid Bacteria (LAB) are gram-positive bacteria that are rod or round in shape, do not form spores, and do not have cytochromes. LAB can be isolated from fermented food products. LAB is useful to inhibit the growth of pathogenic bacteria so that they are potential antibacterial sources. The purpose of this study was to isolate LAB from the traditional food Dali Ni Horbo, continued with characterization, antimicrobial testing, and sensitivity testing towards several antibiotics. From the results of the study, the number of colonies obtained was 2.4 x 107 CFU/mL. 6 isolates were randomly selected, coded as isolates UPDH1, UPDH2, UPDH3, UPDH4, UPDH5, UPDH6, respectively. Biochemical characterization showed that the isolates were gram-positive and catalase-negative bacteria. Results of antibiotic sensitivity testing using 6 antibiotics, namely amoxicillin (AML), erythromycin (E), oxacillin (OX), ofloxacin (OFX), cefotaxime (CTX), and gentamicin (CN), showed that all the isolates were resistant to CTX. The UPDH1 isolate was resistant to AML and OX. The UPDH3, UPDH5, and UPDH6 isolates were resistant to AML, and the UPDH4 isolate was resistant to E, OFX, CTX, CN, and OX. Antimicrobial tests used the disk diffusion method against Escherichia coli and Staphylococcus aureus. The UPDH2 isolate, gave an inhibition zone of 6.7 mm, and UPDH5 and UPDH6 gave inhibition zones of 7.6 mm and 8.5 mm respectively, against E. coli. Microbial inhibition tests against S. aureus by UPDH1, UPDH2, UPDH5 and UPDH6 gave inhibition zones of 13.5 mm, 9.0 mm, 12.1 mm and 12 mm respectively. From the results in this research it can be concluded that the traditional food Dali Ni Horbo is a potential source of probiotics.","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130830805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
α-amilase is one of digestive enzyme that hydrolize starch to maltose by α-glukosidase and degradation to form a glucose and continue with blood adsorption through villi of small intestine. Consomption of acarbose drug is one of ways for diabetic treatment to inhibit the activity of α-amilase. Tamarind leaves (Tamarindus indica L) herbal benalu api (Scurrula Sp) and herbal putri malu (Mimosa pudica D) regularly used as medical plant with activity of antidiabetic medicine. The aims of this studies was to analyze the potency of three medical plant with form of infusa and ethanol extract from fresh or dried plant to inhibit activity of α-amilase and akarbose used as positive control. Inhibition potency of sample against activity of α-amilase were determine base on maltose produced by of starch hydrolysis α-amilase to reduction dinitrosalicylic acid become 3-amino-5-nitrosalicylic acid and the absorbance were measured with spektrofotometer at 530 nm. Resulted of percentage inhibition against activity of α-amilase were herbal infusa from dried benalu api 85.58 ± 2,93%, infusa of fresh putri malu 87.40 ± 1,81%, and the dried 98,85 ± 0,66%. These results did not significancy different with inhibition of akarbose 93.89 + 0,02%. Infusa herbal of dried benalu api, fresh and dried putri malu were potential cover for acarbose to used as alternative medicine.
{"title":"Analysis of Infusion and Ethanol Extract of Tamarindus indica L, Scurrula Sp, Mimosa pudica D of Fresh and Dry as Amylase Enzyme Inhibitor","authors":"Silvera Devi Sy, Musyirna Rahmah Nst, Ninuk Rodhiatul Jannah","doi":"10.31258/jnat.17.2.25-31","DOIUrl":"https://doi.org/10.31258/jnat.17.2.25-31","url":null,"abstract":"α-amilase is one of digestive enzyme that hydrolize starch to maltose by α-glukosidase and degradation to form a glucose and continue with blood adsorption through villi of small intestine. Consomption of acarbose drug is one of ways for diabetic treatment to inhibit the activity of α-amilase. Tamarind leaves (Tamarindus indica L) herbal benalu api (Scurrula Sp) and herbal putri malu (Mimosa pudica D) regularly used as medical plant with activity of antidiabetic medicine. The aims of this studies was to analyze the potency of three medical plant with form of infusa and ethanol extract from fresh or dried plant to inhibit activity of α-amilase and akarbose used as positive control. Inhibition potency of sample against activity of α-amilase were determine base on maltose produced by of starch hydrolysis α-amilase to reduction dinitrosalicylic acid become 3-amino-5-nitrosalicylic acid and the absorbance were measured with spektrofotometer at 530 nm. Resulted of percentage inhibition against activity of α-amilase were herbal infusa from dried benalu api 85.58 ± 2,93%, infusa of fresh putri malu 87.40 ± 1,81%, and the dried 98,85 ± 0,66%. These results did not significancy different with inhibition of akarbose 93.89 + 0,02%. Infusa herbal of dried benalu api, fresh and dried putri malu were potential cover for acarbose to used as alternative medicine.","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122975189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-30DOI: 10.31258/jnat.17.2.16-24
Muhamad Rokim, A. Zamri, H. Y. Teruna
Flavonols 2-(3,4,5-dimethoxyphenyli)-3-hydroxy-4h-chromen-4-on) derivatives of 2'-hydroxycalone have been synthesized under basic condition (KOH). The structures of all compounds were characterized based on the interpretation of spectroscopic data including UV, FTIR, NMR and HRMS. Antioxidant activity was evaluated using the DPPH assay and anticancer activity was evaluated using the MTS assay which showed that the flavonol 2'-hydroxycalone derivative was potentially active as antioxidants and anticancer with IC50 values <1000 µg / mL.
{"title":"Synthesis, Test of Antioxidant and Anticancer Activities Flavonol 2- (3,4,5-Dimethoxyphenyli) -3-Hydroxy-4H-Chromen-4-On","authors":"Muhamad Rokim, A. Zamri, H. Y. Teruna","doi":"10.31258/jnat.17.2.16-24","DOIUrl":"https://doi.org/10.31258/jnat.17.2.16-24","url":null,"abstract":"Flavonols 2-(3,4,5-dimethoxyphenyli)-3-hydroxy-4h-chromen-4-on) derivatives of 2'-hydroxycalone have been synthesized under basic condition (KOH). The structures of all compounds were characterized based on the interpretation of spectroscopic data including UV, FTIR, NMR and HRMS. Antioxidant activity was evaluated using the DPPH assay and anticancer activity was evaluated using the MTS assay which showed that the flavonol 2'-hydroxycalone derivative was potentially active as antioxidants and anticancer with IC50 values <1000 µg / mL.","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"46 36","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113993640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Marlinda, H. Y. Teruna, N. Pratiwi, A. Ardhi, S. Saryono
Fusarium oxysporum LBKURCC41 is one of endophyte fungi which is able to produce secondary metabolites. The purpose of this research was to discover an antioxidant agent from F. oxysporum LBKURCC41extract that was fermented in Huang medium for 15 d with of corn and potato with particle size of 80 mesh as carbon sources. The ethyl acetate extract from the cultured medium showed four dominant component with retentiont time of 3.24, 3.44, 17.02 and 18.889 min. The F. oxysporum LBKURCC41 extract containing compounds with functional group O-H, C-H and C-O, and it had a IC50 value of 435,157±12,009a.
{"title":"Antioxydant Extract of Endophytic Fungi Fusarium oxysporum LBKURCC41","authors":"S. Marlinda, H. Y. Teruna, N. Pratiwi, A. Ardhi, S. Saryono","doi":"10.31258/jnat.17.2.1-9","DOIUrl":"https://doi.org/10.31258/jnat.17.2.1-9","url":null,"abstract":"Fusarium oxysporum LBKURCC41 is one of endophyte fungi which is able to produce secondary metabolites. The purpose of this research was to discover an antioxidant agent from F. oxysporum LBKURCC41extract that was fermented in Huang medium for 15 d with of corn and potato with particle size of 80 mesh as carbon sources. The ethyl acetate extract from the cultured medium showed four dominant component with retentiont time of 3.24, 3.44, 17.02 and 18.889 min. The F. oxysporum LBKURCC41 extract containing compounds with functional group O-H, C-H and C-O, and it had a IC50 value of 435,157±12,009a.","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130435782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-30DOI: 10.31258/jnat.17.2.32-42
N. Muslimah, U. Pato, S. Saryono
Sugarcane (Saccharum officinarum) is a plant that is widely used as raw material for making sugar and can be enjoyed directly by extracting the juice. While the pulp has not been utilized and is often disposed of as waste. This study aims to make compost using Trichoderma (LBKURCC1 and LBKURCC2) and Pseudomonas (LBKURCC54 and LBKURCC59) as bioactivators. The composting process is done by mixing bagasse and chicken manure with a ratio of 2: 1 and adding isolate fungus Trichoderma sp. and Pseudomonas stutzeri bacteria as activator with several combinations on compost media with 5 treatment. Compost treatment includes P0 (control), P1 (J1_J2_B1), P2 (J1_J2_B2), P3 (J1_B1_B2), P4 (J2_B1_B2) and P5 (J1_J2_B1), P3 (J1_J2_B1_B2). The process of composting bagasse is done in a poly bag. Analyzed of compost quality was determined based on parameters of temperature, water content and the levels of C/N ratio observed for 18 days. In this study, the use of Trichoderma (LBKURCC1 and LBKURCC2) and Pseudomonas (LBKURCC54 and LBKURCC59) as bioactivators in a mixture of compost material gave a significant difference to control at the C/N ratio with a value of P> 0.05. The highest C/N ratio is from P5 (28.43) and the lowest is from P2 (5.17).
{"title":"Sugarcane-Bagasse Trichokompos Optimalization using Trichoderma sp. (LBKURCC1 and LBKURCC2) and Pseudomonas szutzeri (LBKURCC54 dan LBKURCC59)","authors":"N. Muslimah, U. Pato, S. Saryono","doi":"10.31258/jnat.17.2.32-42","DOIUrl":"https://doi.org/10.31258/jnat.17.2.32-42","url":null,"abstract":"Sugarcane (Saccharum officinarum) is a plant that is widely used as raw material for making sugar and can be enjoyed directly by extracting the juice. While the pulp has not been utilized and is often disposed of as waste. This study aims to make compost using Trichoderma (LBKURCC1 and LBKURCC2) and Pseudomonas (LBKURCC54 and LBKURCC59) as bioactivators. The composting process is done by mixing bagasse and chicken manure with a ratio of 2: 1 and adding isolate fungus Trichoderma sp. and Pseudomonas stutzeri bacteria as activator with several combinations on compost media with 5 treatment. Compost treatment includes P0 (control), P1 (J1_J2_B1), P2 (J1_J2_B2), P3 (J1_B1_B2), P4 (J2_B1_B2) and P5 (J1_J2_B1), P3 (J1_J2_B1_B2). The process of composting bagasse is done in a poly bag. Analyzed of compost quality was determined based on parameters of temperature, water content and the levels of C/N ratio observed for 18 days. In this study, the use of Trichoderma (LBKURCC1 and LBKURCC2) and Pseudomonas (LBKURCC54 and LBKURCC59) as bioactivators in a mixture of compost material gave a significant difference to control at the C/N ratio with a value of P> 0.05. The highest C/N ratio is from P5 (28.43) and the lowest is from P2 (5.17).","PeriodicalId":404489,"journal":{"name":"Jurnal Natur Indonesia","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128528432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-30DOI: 10.31258/jnat.17.2.10-15
Deri Islami, H. Y. Teruna, Yum Eryanti
Plectranthus amboinicus (Lour.) is a perennial plant belonging to the family of Lamiaceae which have been widely used in traditional herbal medicine. In the present study, the extracts of the leaves of P. amboinicus that was extracted by different solvents were evaluated for their antioxidant and antibacterial properties. The fresh leaves of the plant were extracted using subsequent methanol, n-hexane and dichloromethane. The antioxidant activity was determined by DPPH free radical scavenging assay and the antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus by agar well diffusion assay. The results of antioxidant activity test showed that dichloromethane extract of the leaves had the highest antioxidant activity among all with IC50 value of 50.2 μg/mL Methanol and n-hexane extracts were still active in DPPH with IC50 value of 71.3 μg/mL and 205.9 μg/mL, respectively. Furthermore, the dichloromethane and methanol ektracts have shown a promising antibacterial activity in E. coli. The findings indicated that dichloromethane and methanol extracts of the leaves of P. amboinicus were potential further study as herbal medicine as they showed high antioxidant and antibacterial activities.
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