Pub Date : 2014-08-20DOI: 10.2142/BIOPHYS.54.S330_2
Hirofumi Suzuki
{"title":"Browsing searching, and Comparing 3D electron microscopy data(PDBJ: Protein Data Bank Japan,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"Hirofumi Suzuki","doi":"10.2142/BIOPHYS.54.S330_2","DOIUrl":"https://doi.org/10.2142/BIOPHYS.54.S330_2","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114140630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/biophys.54.S265_2
M. Patti, Rieko Kamii, T. Ishizuka, H. Yawo
{"title":"3P098 Engineering of Channelrhodopsin with Specific Ion Selectivity(03. Membrane proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"M. Patti, Rieko Kamii, T. Ishizuka, H. Yawo","doi":"10.2142/biophys.54.S265_2","DOIUrl":"https://doi.org/10.2142/biophys.54.S265_2","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117308091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"3P190 Shear banding in an F-actin solution(Cell biology,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"Kunita Itsuki, Sato Katsuhiko, Tanaka Yoshimi, Takikawa Yoshinori, Orihara Hiroshi, Nakagaki Toshiyuki","doi":"10.2142/BIOPHYS.54.S280_4","DOIUrl":"https://doi.org/10.2142/BIOPHYS.54.S280_4","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115804955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/biophys.54.S256_1
Jing Cheng, Yuichi Hanada, H. Kondo, S. Tsuda
Antifreeze proteins (AFPs) facilitate cold-survival of the organisms living under freezing environment by inhibiting ice crystal growth. In this study, we focused on a fungal AFP denoted TisAFP secreted from a psychrophilic snow-mold fungus, Typhula ishikariensis. It reported seven isoforms of TisAFP that share a high sequence identity. Significantly, fluorescencebased ice plane affinity (FIPA) analysis showed that the ice binding specificity of TisAFP8 is quite different from the others. To clarify what determines the uniqueness of TisAFP8, we performed mutagenesis experiments on TisAFP8, especially on the residues constructing its putative ice-binding site. The data suggested an importance of inward pointing residues not just outward pointing residues of TisAFP8.
{"title":"3P043 Mutagenesis study of an antifreeze protein isoform from a snow-mold fungus, Typhula ishikariensis(01B. Protein: Structure & Function,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"Jing Cheng, Yuichi Hanada, H. Kondo, S. Tsuda","doi":"10.2142/biophys.54.S256_1","DOIUrl":"https://doi.org/10.2142/biophys.54.S256_1","url":null,"abstract":"Antifreeze proteins (AFPs) facilitate cold-survival of the organisms living under freezing environment by inhibiting ice crystal growth. In this study, we focused on a fungal AFP denoted TisAFP secreted from a psychrophilic snow-mold fungus, Typhula ishikariensis. It reported seven isoforms of TisAFP that share a high sequence identity. Significantly, fluorescencebased ice plane affinity (FIPA) analysis showed that the ice binding specificity of TisAFP8 is quite different from the others. To clarify what determines the uniqueness of TisAFP8, we performed mutagenesis experiments on TisAFP8, especially on the residues constructing its putative ice-binding site. The data suggested an importance of inward pointing residues not just outward pointing residues of TisAFP8.","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"117 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115878526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/BIOPHYS.54.S250_5
S. Ito, Y. Okamoto, S. Irle
{"title":"3P011 Implementation of Replica-Exchange Umbrella Sampling to the DFTB+ Simulation Package(01A. Protein: Structure,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"S. Ito, Y. Okamoto, S. Irle","doi":"10.2142/BIOPHYS.54.S250_5","DOIUrl":"https://doi.org/10.2142/BIOPHYS.54.S250_5","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114990163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/biophys.54.S170_1
Tulum Isil, M. Yabe, A. Uenoyama, M. Miyata
{"title":"1P175 Localization and roles of F_1-ATPase subunit homologs and P42 of Mycoplasma mobile revealed by gene manipulation(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"Tulum Isil, M. Yabe, A. Uenoyama, M. Miyata","doi":"10.2142/biophys.54.S170_1","DOIUrl":"https://doi.org/10.2142/biophys.54.S170_1","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123313110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/BIOPHYS.54.S182_5
R. Mutoh, Hisako Kubota-Kawai, M. Nowaczyk, M. Rögner, Hideaki Tanaka, T. Ikegami, G. Kurisu
{"title":"1P251 X-ray structure and NMR analysis of the electron transfer complex between photosystem I and ferredoxin(18B. Photobiology:Photosynthesis,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"R. Mutoh, Hisako Kubota-Kawai, M. Nowaczyk, M. Rögner, Hideaki Tanaka, T. Ikegami, G. Kurisu","doi":"10.2142/BIOPHYS.54.S182_5","DOIUrl":"https://doi.org/10.2142/BIOPHYS.54.S182_5","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123665282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/biophys.54.S239_1
T. Nozaki, T. Tani, Sachiko Tamura, T. Nagai, K. Maeshima
Genomic DNA must be organized three dimensionally in the cell to utilize genome information. Our recent studies demonstrated the nucleosome fiber is irregularly folded in the cell without the 30-nm chromatin fiber. Using newly developed single nucleosome imaging, we identified numerous compact nucleosome clusters (chromatin domains) in both interphase chromatin and mitotic chromosomes in living mammalian cells. Subsequently, we also observed that nucleosomes dynamically moved around in the chromatin domains. Furthermore, we detected that some chemical perturbations could change the chromatin domains and nucleosome dynamics. These findings provide a novel insight into the genome organization and dynamics in living mammalian cells.
{"title":"2P265 Super-resolution imaging of chromatin domains in living mammalian cells(21B. Genome biology:Genome structure,Poster)","authors":"T. Nozaki, T. Tani, Sachiko Tamura, T. Nagai, K. Maeshima","doi":"10.2142/biophys.54.S239_1","DOIUrl":"https://doi.org/10.2142/biophys.54.S239_1","url":null,"abstract":"Genomic DNA must be organized three dimensionally in the cell to utilize genome information. Our recent studies demonstrated the nucleosome fiber is irregularly folded in the cell without the 30-nm chromatin fiber. Using newly developed single nucleosome imaging, we identified numerous compact nucleosome clusters (chromatin domains) in both interphase chromatin and mitotic chromosomes in living mammalian cells. Subsequently, we also observed that nucleosomes dynamically moved around in the chromatin domains. Furthermore, we detected that some chemical perturbations could change the chromatin domains and nucleosome dynamics. These findings provide a novel insight into the genome organization and dynamics in living mammalian cells.","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124175501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/BIOPHYS.54.S178_5
Hiroki Kojima, Keisuke Hotta, Y. Hasegawa, S. Kawato
{"title":"1P227 Acute Modulation of long-term potentiation of Pyramidal Neurons by Hippocampal-derived Estrogen(15. Neuroscience & Sensory systems,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"Hiroki Kojima, Keisuke Hotta, Y. Hasegawa, S. Kawato","doi":"10.2142/BIOPHYS.54.S178_5","DOIUrl":"https://doi.org/10.2142/BIOPHYS.54.S178_5","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126512314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-08-20DOI: 10.2142/BIOPHYS.54.S226_2
Hajime Fukuoka, Tomoko Horigome, Y. Inoue, Hiroto Takahashi, A. Ishijima
{"title":"2P188 Trial for detecting the activation and inactivation of chemoreceptor array in a single E. coli cell(12. Cell biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))","authors":"Hajime Fukuoka, Tomoko Horigome, Y. Inoue, Hiroto Takahashi, A. Ishijima","doi":"10.2142/BIOPHYS.54.S226_2","DOIUrl":"https://doi.org/10.2142/BIOPHYS.54.S226_2","url":null,"abstract":"","PeriodicalId":409321,"journal":{"name":"Seibutsu Butsuri","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129463892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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