Pub Date : 2020-01-01DOI: 10.26577/ijbch.2020.v13.i2.13
A. Martusevich, O. Zhdanova
Ab st ract. The aim of the current work was to clarify the dynamics in cry st allogenic and initiating activity of patients’ biological fluids in alveococcosis during surgery and in the late po st operative period. The material of the st udy included samples of saliva of 22 patients treated for alveococcosis. Sampling of biological fluids was managed at the admission and prior to discharge from the hospital. Subsequently slides were prepared using teziocry st alloscopy technique that combines the st udy of the cry st al forming activity of mixed saliva (classical cry st alloscopy) and its initiating properties considering 0.9% sodium chloride solution as basis sub st ance (comparative teziography). Criterial evaluation of the results of cry st alloscopic and tezigraphic te st s was carried out using own sy st em of parameters. The data of visual morphometry of microslides of dehydrated saliva was supplemented with spectrophotometric analysis of cry st alloscopic and tezigraphic facies performed on PowerWave XS microplate spectrophotometer (USA) at wavelengths of 300, 350 and 400 nm. It was noted that surgical treatment leads to partial normalization of physical and
Ab st。当前工作的目的是阐明在手术期间和手术后期肺泡球菌病患者的生物体液的同种异体和启动活性的动态。第一项研究的材料包括22例接受肺泡球菌病治疗的患者的唾液样本。在入院时和出院前对生物液体进行了采样。随后，我们使用teziocry - st - alloscopy技术制备了载玻片，该技术结合了混合唾液的泪质形成活性(经典泪质alloscopy)和以0.9%氯化钠溶液为基础物质的起始性质(比较teziography)的研究。采用自己的参数体系，对异体和纯石试验结果进行了标准评价。在脱水唾液微玻片的视觉形态测量数据的基础上，采用美国PowerWave XS微孔板分光光度计在300、350和400 nm波长下对唾液的异体和泥质相进行分光光度计分析。有人指出，手术治疗导致身体和
{"title":"Dynamics of some physical and chemical properties оf biological fluids in surgical treatment оf alveolar hydatid disease of liver","authors":"A. Martusevich, O. Zhdanova","doi":"10.26577/ijbch.2020.v13.i2.13","DOIUrl":"https://doi.org/10.26577/ijbch.2020.v13.i2.13","url":null,"abstract":"Ab st ract. The aim of the current work was to clarify the dynamics in cry st allogenic and initiating activity of patients’ biological fluids in alveococcosis during surgery and in the late po st operative period. The material of the st udy included samples of saliva of 22 patients treated for alveococcosis. Sampling of biological fluids was managed at the admission and prior to discharge from the hospital. Subsequently slides were prepared using teziocry st alloscopy technique that combines the st udy of the cry st al forming activity of mixed saliva (classical cry st alloscopy) and its initiating properties considering 0.9% sodium chloride solution as basis sub st ance (comparative teziography). Criterial evaluation of the results of cry st alloscopic and tezigraphic te st s was carried out using own sy st em of parameters. The data of visual morphometry of microslides of dehydrated saliva was supplemented with spectrophotometric analysis of cry st alloscopic and tezigraphic facies performed on PowerWave XS microplate spectrophotometer (USA) at wavelengths of 300, 350 and 400 nm. It was noted that surgical treatment leads to partial normalization of physical and","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69016943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.26577/ijbch.2020.v13.i2.07
O. Khamdiyeva, Z. B. Tileulesa, G. Baratzhanova, S. S. Murzataeva, E. Khussainova, S. Abdikerim, A. Childebayeva, L. Djansugurova
Epilepsy is a common manifestation of mitochondrial disorders, in addition mitochondrial oxidative stress may play an important role in epileptogenesis since it affects the excitability of neurons. Mitochondrial diseases are often difficult to diagnose unless the symptoms are clearly identified as a part of a specific mitochondrial mutation. The use of next-generation sequencing would lead to the rapid identification of genes associated with epilepsy syndromes. In this study, we evaluate the applicability of the NGS method for diagnosis of mitochondrial epilepsy by clinicians and laboratories in Kazakhstan. We performed complete mitochondrial genome sequencing on the Illumina MiSeq platform for 6 patients with epilepsy. Using the MITOMAP and HmtDB databases, we identified three pathogenic variants (MT-ND1 m.3697G> A, m.5628T> C and m.7547T> C) leading to the development of epilepsy, additionally we found 6 variable sites (m.5586C> T, m.10095C> T, 514_515delCA, 16180_16181delAA, C514.CACACA, A955. ACCCC), the clinic of which was not previously mentioned in the literature. Our preliminary study suggests that mitochondrial genes potentially play a role in the pathogenesis of epilepsy and mutations in these genes cause various forms of epilepsy. It is necessary to elucidate the main mechanisms and participation of variants of mtDNA haplogroups in the development of epilepsy to apply the NGS method in diagnosis of mitochondrial epilepsy.
{"title":"Next Generation Sequencing in diagnosis of mitochondrial epilepsy","authors":"O. Khamdiyeva, Z. B. Tileulesa, G. Baratzhanova, S. S. Murzataeva, E. Khussainova, S. Abdikerim, A. Childebayeva, L. Djansugurova","doi":"10.26577/ijbch.2020.v13.i2.07","DOIUrl":"https://doi.org/10.26577/ijbch.2020.v13.i2.07","url":null,"abstract":"Epilepsy is a common manifestation of mitochondrial disorders, in addition mitochondrial oxidative stress may play an important role in epileptogenesis since it affects the excitability of neurons. Mitochondrial diseases are often difficult to diagnose unless the symptoms are clearly identified as a part of a specific mitochondrial mutation. The use of next-generation sequencing would lead to the rapid identification of genes associated with epilepsy syndromes. In this study, we evaluate the applicability of the NGS method for diagnosis of mitochondrial epilepsy by clinicians and laboratories in Kazakhstan. We performed complete mitochondrial genome sequencing on the Illumina MiSeq platform for 6 patients with epilepsy. Using the MITOMAP and HmtDB databases, we identified three pathogenic variants (MT-ND1 m.3697G> A, m.5628T> C and m.7547T> C) leading to the development of epilepsy, additionally we found 6 variable sites (m.5586C> T, m.10095C> T, 514_515delCA, 16180_16181delAA, C514.CACACA, A955. ACCCC), the clinic of which was not previously mentioned in the literature. Our preliminary study suggests that mitochondrial genes potentially play a role in the pathogenesis of epilepsy and mutations in these genes cause various forms of epilepsy. It is necessary to elucidate the main mechanisms and participation of variants of mtDNA haplogroups in the development of epilepsy to apply the NGS method in diagnosis of mitochondrial epilepsy.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69016266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.26577/ijbch.2020.v13.i2.10
O. A. Iksan, I. Kisselev, A. Garshin, L. Skvortsova, Zhassulan Zhaniyazov, M. A. Alpysbes, Charleston W. K. Chiang, L. Z. Musralina, G. Zhunussova, E. Khussainova
A haplogroup is a group of similar alleles that have a common ancestor in which a mutation has occurred, inherited by all descendants. Haplogroups, particularly from the Y-chromosome (Y-DNA), is widely used in population genetics and genetic genealogy, a science that studies the genetic history of mankind. Recent studies of the Y-chromosome of modern Kazakhs have demonstrated the diversity of the Kazakh gene pool. During the expedition carried out in 2014-2016, clinical material was collected from varios regions of Kazakhstan, representing samples of peripheral blood and buccal scrapings. All representatives of Kazakh nationality were familiarized with informed consent. In total 1623 respondents participated in the study, 169 of whom were representatives from Baiuly tribe of Junior zhuz. We analyzed the provided samples and found that the Baiuly is characterized by 10 haplogroups, the most prevailing of which is the C2 haplogroup (85%).
{"title":"Analysis of the Kazakh tribe Baiuly based on the distribution of the Y-chromosome haplogroups","authors":"O. A. Iksan, I. Kisselev, A. Garshin, L. Skvortsova, Zhassulan Zhaniyazov, M. A. Alpysbes, Charleston W. K. Chiang, L. Z. Musralina, G. Zhunussova, E. Khussainova","doi":"10.26577/ijbch.2020.v13.i2.10","DOIUrl":"https://doi.org/10.26577/ijbch.2020.v13.i2.10","url":null,"abstract":"A haplogroup is a group of similar alleles that have a common ancestor in which a mutation has occurred, inherited by all descendants. Haplogroups, particularly from the Y-chromosome (Y-DNA), is widely used in population genetics and genetic genealogy, a science that studies the genetic history of mankind. Recent studies of the Y-chromosome of modern Kazakhs have demonstrated the diversity of the Kazakh gene pool. During the expedition carried out in 2014-2016, clinical material was collected from varios regions of Kazakhstan, representing samples of peripheral blood and buccal scrapings. All representatives of Kazakh nationality were familiarized with informed consent. In total 1623 respondents participated in the study, 169 of whom were representatives from Baiuly tribe of Junior zhuz. We analyzed the provided samples and found that the Baiuly is characterized by 10 haplogroups, the most prevailing of which is the C2 haplogroup (85%).","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69016925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.26577/ijbch.2020.v13.i2.01
T. Nurkenov, A. Tsoy, G. Zhusupova, F. Olzhayev, M.Zh. Shertay, T. Shalakhmetova, J.C.‐Y. Lee, S. Askarova
One of the important directions in the treatment of ischemic stroke (IS) consequences is revascularization of the damaged brain areas including decreasing acute hypoxia and oxidative stress that occur in the ischemic tissue due to reperfusion syndrome after restoration of blood flow. Plant polyphenols are promising candidates capable of exerting a pronounced antioxidant and neuroprotective effects. There are a number of wild plants growing on the territory of Kazakhstan, and one of these plants containing significant number of polyphenols is Limonium gmelinii (L. gmelinii, genus Limonium Mill). In our study we have applied middle cerebral artery occlusion (MCAO) method to induce focal ischemic cerebral stroke in male Wistar rats. The results of assessment of sensorimotor functions in laboratory animals showed that MCAO resulted in sensorimotor deficiency. At the same time, partial recoveries of sensorimotor functions were observed in animals that were treated with extract of L. gmelinii after stroke compared to untreated animals. Similarly, histological analysis of the damaged brain regions has revealed focal coagulation necrosis with clearly visualized damaged regions in animals with MCAO, whereas brain tissue of animals exposed to L. gmelinii extract after stroke induction was partially restored. Our results demonstrate that extract of L. gmelinii possesses neuroprotective properties that require further investigations.
{"title":"Therapeutic effectiveness of Limonium gmelinii extract in experimentally – induced ischemic brain damage in vivo","authors":"T. Nurkenov, A. Tsoy, G. Zhusupova, F. Olzhayev, M.Zh. Shertay, T. Shalakhmetova, J.C.‐Y. Lee, S. Askarova","doi":"10.26577/ijbch.2020.v13.i2.01","DOIUrl":"https://doi.org/10.26577/ijbch.2020.v13.i2.01","url":null,"abstract":"One of the important directions in the treatment of ischemic stroke (IS) consequences is revascularization of the damaged brain areas including decreasing acute hypoxia and oxidative stress that occur in the ischemic tissue due to reperfusion syndrome after restoration of blood flow. Plant polyphenols are promising candidates capable of exerting a pronounced antioxidant and neuroprotective effects. There are a number of wild plants growing on the territory of Kazakhstan, and one of these plants containing significant number of polyphenols is Limonium gmelinii (L. gmelinii, genus Limonium Mill). In our study we have applied middle cerebral artery occlusion (MCAO) method to induce focal ischemic cerebral stroke in male Wistar rats. The results of assessment of sensorimotor functions in laboratory animals showed that MCAO resulted in sensorimotor deficiency. At the same time, partial recoveries of sensorimotor functions were observed in animals that were treated with extract of L. gmelinii after stroke compared to untreated animals. Similarly, histological analysis of the damaged brain regions has revealed focal coagulation necrosis with clearly visualized damaged regions in animals with MCAO, whereas brain tissue of animals exposed to L. gmelinii extract after stroke induction was partially restored. Our results demonstrate that extract of L. gmelinii possesses neuroprotective properties that require further investigations.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69016187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.26577/ijbch.2020.v13.i2.04
A. Belkozhayev, R. Niyazova, C. Wilson
Trinucleotide repeat disorders are a group of predominantly inherited neurological diseases caused by the expansion of repetitive sequences. miRNAs play major roles in transcriptional regulation and are expressed selectively and abundantly in the central nervous system. In the present research, MirTarget program predicted the miRNA-binding sites in mRNAs of genes with trinucleotide repeats. The MirTarget programme determines the following features of binding: the start of the initiation of miRNA binding to mRNAs; the localization of miRNA binding sites in 5’UTRs, CDSs and 3’UTRs; the free energy of binding; and the schemes of nucleotide interactions between miRNAs and mRNAs. In coding sequences the binding sites of ID00372.5p-miR with mRNA of ATXN2, FMN2 and MN1 genes having CAG (Q) repeats show the highest free binding energy. The mRNA of ADRBK1, BRSK2, C11orf87 and FMR1 genes have ID01508.5p-miR binding sites in 5’UTR with CGG repeated regions. Also, the binding sites of ID00296.3p-miR and ID01702.3p-miR in 5’UTR of BLMH gene interacted with CCG repeats. DMPK gene with CUG repeated regions have ID00522.5p-miR binding sites in 3’UTR. Based on these results, the interactions of ID00372.5p-miR, ID01508.5p-miR, ID00296.3p-miR, ID01702.3p-miR and ID00522.5pmiR and their target genes ATXN2, FMN2, MN1, ADRBK1, BRSK2, C11orf87, FMR1, BLMH and DMPK can be used for developing methods for diagnosing and therapeutic targets for neurological disorders.
{"title":"The features of novel miRNAs interaction with mRNA candidate genes having trinucleotide repeats in coding sequences and untranslated regions","authors":"A. Belkozhayev, R. Niyazova, C. Wilson","doi":"10.26577/ijbch.2020.v13.i2.04","DOIUrl":"https://doi.org/10.26577/ijbch.2020.v13.i2.04","url":null,"abstract":"Trinucleotide repeat disorders are a group of predominantly inherited neurological diseases caused by the expansion of repetitive sequences. miRNAs play major roles in transcriptional regulation and are expressed selectively and abundantly in the central nervous system. In the present research, MirTarget program predicted the miRNA-binding sites in mRNAs of genes with trinucleotide repeats. The MirTarget programme determines the following features of binding: the start of the initiation of miRNA binding to mRNAs; the localization of miRNA binding sites in 5’UTRs, CDSs and 3’UTRs; the free energy of binding; and the schemes of nucleotide interactions between miRNAs and mRNAs. In coding sequences the binding sites of ID00372.5p-miR with mRNA of ATXN2, FMN2 and MN1 genes having CAG (Q) repeats show the highest free binding energy. The mRNA of ADRBK1, BRSK2, C11orf87 and FMR1 genes have ID01508.5p-miR binding sites in 5’UTR with CGG repeated regions. Also, the binding sites of ID00296.3p-miR and ID01702.3p-miR in 5’UTR of BLMH gene interacted with CCG repeats. DMPK gene with CUG repeated regions have ID00522.5p-miR binding sites in 3’UTR. Based on these results, the interactions of ID00372.5p-miR, ID01508.5p-miR, ID00296.3p-miR, ID01702.3p-miR and ID00522.5pmiR and their target genes ATXN2, FMN2, MN1, ADRBK1, BRSK2, C11orf87, FMR1, BLMH and DMPK can be used for developing methods for diagnosing and therapeutic targets for neurological disorders.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69016210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.26577/ijbch.2020.v13.i2.05
A. Sarıkaya, Seydi Ahmet Kavakli
Campanula is one of the largest genus of the Campanulaceae family, represented by approximately 115 species in Turkey and is spread through Iran-Turan, Eastern Mediterranean and Mediterranean phytogeographical regions. The aim of the current study was to determine some morphological features, phenological characteristics and leaf-flower volatile components of Campanula rapunculus var. rapunculus collected in different sites of Uludag Mountain (Bursa). The average plant height was calculated as 37.21 cm, number of corolla was 2.4, the length of the corolla was 2.0 cm, calyx lobe length was 1.36 cm, leaf width was 1.7 cm and leaf length was 4.31 cm. Considering phenological features, it was measured that the first flowering ends in May, full flowering ends in June and late flowering ends in late July. A total of 32 different volatile components were detected in Campanula rapunculus. With the aid of gas chromatographymass spectroscopy (GC-MS) of volatile components by solid phase microextraction technique (SPME), 3-Methylbutanal (10.87%), cis-3-Hexene-1-ol (9.85%) and 3-Octanol (9.62%) were identified as major components. In addition to its medicinal benefits, studies on Campanula genus, considered as food and ornamental plants, should be increased and its value should be revealed.
{"title":"Some morphological and phenological characteristics and volatile components of Campanula rapunculus L. var. rapunculus","authors":"A. Sarıkaya, Seydi Ahmet Kavakli","doi":"10.26577/ijbch.2020.v13.i2.05","DOIUrl":"https://doi.org/10.26577/ijbch.2020.v13.i2.05","url":null,"abstract":"Campanula is one of the largest genus of the Campanulaceae family, represented by approximately 115 species in Turkey and is spread through Iran-Turan, Eastern Mediterranean and Mediterranean phytogeographical regions. The aim of the current study was to determine some morphological features, phenological characteristics and leaf-flower volatile components of Campanula rapunculus var. rapunculus collected in different sites of Uludag Mountain (Bursa). The average plant height was calculated as 37.21 cm, number of corolla was 2.4, the length of the corolla was 2.0 cm, calyx lobe length was 1.36 cm, leaf width was 1.7 cm and leaf length was 4.31 cm. Considering phenological features, it was measured that the first flowering ends in May, full flowering ends in June and late flowering ends in late July. A total of 32 different volatile components were detected in Campanula rapunculus. With the aid of gas chromatographymass spectroscopy (GC-MS) of volatile components by solid phase microextraction technique (SPME), 3-Methylbutanal (10.87%), cis-3-Hexene-1-ol (9.85%) and 3-Octanol (9.62%) were identified as major components. In addition to its medicinal benefits, studies on Campanula genus, considered as food and ornamental plants, should be increased and its value should be revealed.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69016257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-09DOI: 10.26577/ijbch-2019-1-i22
M. Gharibi-Tehrani, A. Elhamirad, E. Azarpazhooh, A. Pedramnia, P. Sharayei, Khorasan Razavi Agricultural
Onion by-products, a waste generated from fruit processing industry, is a potential source of phenolic compounds that are known for their anti-oxidative properties. The influence of pulsed electric field (PEF) treatment on the bioactive compounds from onion by-products at different pulse voltage (PV); 2000, 4000, 6000Vand number of pulse (NP); 40, 50, 60 has been investigated. Response surface methodology, based on a Face-Centered Experimental Design (FCED) was used to determine optimal PEF treatment and optimize extraction yield, antioxidant strength, total phenolic compound (TPC),and quercetin content. The experimental data were fitted to a second‐order polynomial equation and also profiled into the corresponding 3‐D contour plots. Optimal extraction conditions were as follows:PV were 4102.97 V and and NP 51.43. Under these conditions, TPC, DPPH, FRAP,Quercetin and extraction yield were 48.912 ± 6 mg/kg, 50.366 ± 1 %, 465.414 ± 5 μmFe2/l, 31.761 ± 0.5 mg/100g and 88.107 ± 1% ; respectively and matching well with the predicted value. The results demonstrated that PEF could be a very effective method for continuous extraction of natural compounds.
{"title":"Natural valuable compound extraction from onion by-products using a pulsed electric field","authors":"M. Gharibi-Tehrani, A. Elhamirad, E. Azarpazhooh, A. Pedramnia, P. Sharayei, Khorasan Razavi Agricultural","doi":"10.26577/ijbch-2019-1-i22","DOIUrl":"https://doi.org/10.26577/ijbch-2019-1-i22","url":null,"abstract":"Onion by-products, a waste generated from fruit processing industry, is a potential source of phenolic compounds that are known for their anti-oxidative properties. The influence of pulsed electric field (PEF) treatment on the bioactive compounds from onion by-products at different pulse voltage (PV); 2000, 4000, 6000Vand number of pulse (NP); 40, 50, 60 has been investigated. Response surface methodology, based on a Face-Centered Experimental Design (FCED) was used to determine optimal PEF treatment and optimize extraction yield, antioxidant strength, total phenolic compound (TPC),and quercetin content. The experimental data were fitted to a second‐order polynomial equation and also profiled into the corresponding 3‐D contour plots. Optimal extraction conditions were as follows:PV were 4102.97 V and and NP 51.43. Under these conditions, TPC, DPPH, FRAP,Quercetin and extraction yield were 48.912 ± 6 mg/kg, 50.366 ± 1 %, 465.414 ± 5 μmFe2/l, 31.761 ± 0.5 mg/100g and 88.107 ± 1% ; respectively and matching well with the predicted value. The results demonstrated that PEF could be a very effective method for continuous extraction of natural compounds.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48555367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-09DOI: 10.26577/ijbch-2019-1-i13
P. Ramezannezhad, S. Heidari-Soureshjani, T. Suhan, Usa Oncology
: Myocardial hypoxia is one of the main complications of myocardial ischemic injury which have high morbidity and mortality. The aim of this review is to investigate the protective effects of medicinal plants in myocardial hypoxia. The words cardiomyocytes alongside with hypox* or myocardial hypoxia, in combination with some herbal terms such as medicinal plant, phyto* and herb*, were used to search for relevant publications indexed in the Institute for Scientific Information (ISI) and PubMed. Finally after all revisions, 74 articles were included in this study. Available evidence shows that certain medicinal plants and herbal derivatives can exert their myocardial protective effects against hypoxia using several pathways. These mechanisms include antioxidant properties such as savaging reactive oxygen species (ROS) and activation antioxidant pathways, suppressing pro-apoptotic genes and regulate apoptosis pathways, regulating autophagy and related pathways, reducing inflammation and suppress proinflammatory cytokines and pathways, inhibiting intracellular Ca 2+ influx, antiplatelet aggregation, stimulating the adenosine triphosphate (ATP) generation in mitochondria and mitochondrial respiration, promoting autophagy, regulating hypoxia-inducible factor 1-alpha (HIF-1α) expression, decreasing the expression of angiotensin, reducing cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB) and up-regulation of epoxyeicosatrienoic acids (EETs). Thus, clinicians can use the medicinal herbs as an effective treatment against myocardial hypoxia. Post-ischemia and chronic treatment of cardioprotection maybe consider as a therapeutic strategies than short term and pre-treatment methods in clinical setting. Nevertheless, more clinical trial studies are required in order to obtain more reliable results.
{"title":"Protective effects of some medicinal plants against myocardial hypoxia","authors":"P. Ramezannezhad, S. Heidari-Soureshjani, T. Suhan, Usa Oncology","doi":"10.26577/ijbch-2019-1-i13","DOIUrl":"https://doi.org/10.26577/ijbch-2019-1-i13","url":null,"abstract":": Myocardial hypoxia is one of the main complications of myocardial ischemic injury which have high morbidity and mortality. The aim of this review is to investigate the protective effects of medicinal plants in myocardial hypoxia. The words cardiomyocytes alongside with hypox* or myocardial hypoxia, in combination with some herbal terms such as medicinal plant, phyto* and herb*, were used to search for relevant publications indexed in the Institute for Scientific Information (ISI) and PubMed. Finally after all revisions, 74 articles were included in this study. Available evidence shows that certain medicinal plants and herbal derivatives can exert their myocardial protective effects against hypoxia using several pathways. These mechanisms include antioxidant properties such as savaging reactive oxygen species (ROS) and activation antioxidant pathways, suppressing pro-apoptotic genes and regulate apoptosis pathways, regulating autophagy and related pathways, reducing inflammation and suppress proinflammatory cytokines and pathways, inhibiting intracellular Ca 2+ influx, antiplatelet aggregation, stimulating the adenosine triphosphate (ATP) generation in mitochondria and mitochondrial respiration, promoting autophagy, regulating hypoxia-inducible factor 1-alpha (HIF-1α) expression, decreasing the expression of angiotensin, reducing cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB) and up-regulation of epoxyeicosatrienoic acids (EETs). Thus, clinicians can use the medicinal herbs as an effective treatment against myocardial hypoxia. Post-ischemia and chronic treatment of cardioprotection maybe consider as a therapeutic strategies than short term and pre-treatment methods in clinical setting. Nevertheless, more clinical trial studies are required in order to obtain more reliable results.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49450722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-09DOI: 10.26577/ijbch-2019-1-i19
S. Turganbay, S. Aidarova, G. Turganbay, Y. Tileuberdi, Sheng-Li Chen
Sulfur nanoparticles display unique physical and chemical feature because of effects such as the quantum size effect, mini size effect, surface effect, and macro-quantum tunnel effect. Therefore, sulfur nanoparticles would present higher efficacies such as removal of heavy metals, radical-scavenging, antimicrobial activity, antioxidant and antitumor activities. They have been using as a fungicide product in agriculture and medicine; to obtain sulfur fertilizers and cosmetics industries, as well as in obtaining nanocomposite lithium batteries; to form stable carbon chains such as C3S and C5S for obtaining hybrid materials with useful properties for gas sensor and catalytic applications. This work presents the results of obtaining sulfur nanoparticles, which can be used in agriculture as a fungicide treatment. Sulfur nanoparticles were obtained by modifying the surface of sulfur by various water-soluble polyelectrolyte (WSP)/surfactant mixtures including NaCMC/CTAB, PDADMAC/SDBS, NaCMC/Triton X-100 (TX100). The size and the structure of sulfur nanoparticles were determined by methods as LSA, XRD, SEM. It is shown that the nanoparticles have a sulfur monoclinic α-form, and their average size is in the range of 100-300 nm. The received products can be considered as perspective forms of application in agriculture and medicine.
{"title":"Synthesis and characterization of sulfur nanoparticles with WSP/surfactants mixtures","authors":"S. Turganbay, S. Aidarova, G. Turganbay, Y. Tileuberdi, Sheng-Li Chen","doi":"10.26577/ijbch-2019-1-i19","DOIUrl":"https://doi.org/10.26577/ijbch-2019-1-i19","url":null,"abstract":"Sulfur nanoparticles display unique physical and chemical feature because of effects such as the quantum size effect, mini size effect, surface effect, and macro-quantum tunnel effect. Therefore, sulfur nanoparticles would present higher efficacies such as removal of heavy metals, radical-scavenging, antimicrobial activity, antioxidant and antitumor activities. They have been using as a fungicide product in agriculture and medicine; to obtain sulfur fertilizers and cosmetics industries, as well as in obtaining nanocomposite lithium batteries; to form stable carbon chains such as C3S and C5S for obtaining hybrid materials with useful properties for gas sensor and catalytic applications. This work presents the results of obtaining sulfur nanoparticles, which can be used in agriculture as a fungicide treatment. Sulfur nanoparticles were obtained by modifying the surface of sulfur by various water-soluble polyelectrolyte (WSP)/surfactant mixtures including NaCMC/CTAB, PDADMAC/SDBS, NaCMC/Triton X-100 (TX100). The size and the structure of sulfur nanoparticles were determined by methods as LSA, XRD, SEM. It is shown that the nanoparticles have a sulfur monoclinic α-form, and their average size is in the range of 100-300 nm. The received products can be considered as perspective forms of application in agriculture and medicine.","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47052216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-09DOI: 10.26577/ijbch-2019-1-i20
A. Omarova, Larissa R. Sassykova, S. Sendilvelan, M. Tulepov, Military Educational
: Catalytic cracking is one of the most common processes in the oil refining industry. It contributes to a significant deepening of the oil refining degree and it is used for the production of high-octane gasolines and diesel fuels from heavy oil fractions, as well as for the production of petrochemical synthesis products. One of the promising methods for the regulation of catalytic properties is the introduction of modifying additives into the catalytic composition. In this work the catalysts on the base of Al 2 O 3 , ZSM catalyst modified by introduction of additives of manganese, lanthanum and zinc have been prepared and studied during the hydrogen-free conversion of hexane, tetradecane and the diesel fraction. The structure and state of the catalyst’s active centers have been studied using a complex of physico-chemical methods (TEM, XPA, TPD). It has been found that catalysts are characterized by the presence of both acid (Bronsted and Lews) and M 0-or Mn + -metal centers. The structure of acid centers can include MnSiO 3, La 5 Si, Mn 15 Si 26 , LaMn 2 Si 2 , La Al 11 O 18 , La 2 Si 2 O 7 , La 3 Si 2 , La 3 Si 2 , functioning as Lews acid sites. The degree of hexane and tetradecane conversion, the composition of the compound form is determined by the length of the hydrocarbon chain and the conditions of the process
{"title":"Processing of model n-alcanes and diesel fraction on the catalyst La-Zn-Mn / Al2O3 + ZSM","authors":"A. Omarova, Larissa R. Sassykova, S. Sendilvelan, M. Tulepov, Military Educational","doi":"10.26577/ijbch-2019-1-i20","DOIUrl":"https://doi.org/10.26577/ijbch-2019-1-i20","url":null,"abstract":": Catalytic cracking is one of the most common processes in the oil refining industry. It contributes to a significant deepening of the oil refining degree and it is used for the production of high-octane gasolines and diesel fuels from heavy oil fractions, as well as for the production of petrochemical synthesis products. One of the promising methods for the regulation of catalytic properties is the introduction of modifying additives into the catalytic composition. In this work the catalysts on the base of Al 2 O 3 , ZSM catalyst modified by introduction of additives of manganese, lanthanum and zinc have been prepared and studied during the hydrogen-free conversion of hexane, tetradecane and the diesel fraction. The structure and state of the catalyst’s active centers have been studied using a complex of physico-chemical methods (TEM, XPA, TPD). It has been found that catalysts are characterized by the presence of both acid (Bronsted and Lews) and M 0-or Mn + -metal centers. The structure of acid centers can include MnSiO 3, La 5 Si, Mn 15 Si 26 , LaMn 2 Si 2 , La Al 11 O 18 , La 2 Si 2 O 7 , La 3 Si 2 , La 3 Si 2 , functioning as Lews acid sites. The degree of hexane and tetradecane conversion, the composition of the compound form is determined by the length of the hydrocarbon chain and the conditions of the process","PeriodicalId":41021,"journal":{"name":"International Journal of Biology and Chemistry","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46399812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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