M. Giammanco, S. Aiello, A. Casuccio, M. Guardia, L. Cicero, R. Puleio, I. Vazzana, G. Tomasello, G. Cassata, G. Leto, D. Majo
Experimental studies have highlighted that the administration of 3,5-diiodo-L-thyronine (T2) to rats fed diets rich in lipids induces a decrease of cholesterol and triglycerides plasma levels and body weight (BW) without inducing liver steatosis. On the basis of these observations we carried out some experimental in vivo studies to assess the effects of multiple high doses of T2 on the pituitary thyroid axis of rats fed diet rich in lipids. Fifteen male Wistar rats were divided into three groups of five animals each. The first group (N group) received standard diet, the second group was fed with a high fat diet (HFD group), while the third group (HFDT2 group) was additionally given T2 intraperitoneally at a dose level of 70 µg/100 g of BW three times a week up to four weeks. At the end of the treatment, blood sample from each animal was collected, centrifuged and the serum was stored at -20°C. The serum concentrations of thyroidstimulating hormone (TSH), triiodothyronine, thyroxine, adrenocorticotropic hormone, triglycerides, cholesterol, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were then determined. In addition, liver of rats was examined by histology in order to assess the presence and degree of steatosis. The administration of T2 to rats fed with a high fat diet suppressed TSH secretion (P=0.013) while no steatosis was observed in the liver of these animals. Our data show that multiple administrations of high doses of T2 to rats fed diets rich in lipid inhibit TSH secretion and prevent the onset of liver steatosis in these animals.
实验研究强调，给富含脂质饮食的大鼠喂食3,5-二碘- l -甲状腺原氨酸(T2)可诱导胆固醇和甘油三酯血浆水平和体重(BW)的降低，而不会引起肝脏脂肪变性。在这些观察的基础上，我们进行了一些体内实验研究，以评估多次高剂量T2对富脂饮食大鼠垂体甲状腺轴的影响。15只雄性Wistar大鼠被分成三组，每组5只。第一组(N组)饲喂标准饲粮，第二组(HFD组)饲喂高脂饲粮，第三组(HFDT2组)在此基础上腹腔注射T2，剂量为70µg/100 g体重，每周3次，持续4周。处理结束时，收集每只动物的血样，离心，血清在-20°C保存。测定血清促甲状腺激素(TSH)、三碘甲状腺原氨酸、甲状腺素、促肾上腺皮质激素、甘油三酯、胆固醇、葡萄糖、丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶的浓度。此外，通过组织学检查大鼠肝脏，以评估脂肪变性的存在和程度。给高脂饮食的大鼠注射T2可抑制TSH分泌(P=0.013)，但这些动物的肝脏未观察到脂肪变性。我们的数据表明，多次给予高剂量的T2给饲粮富含脂质的大鼠抑制TSH分泌，并防止这些动物发生肝脏脂肪变性。
{"title":"Effects of 3,5-diiodo-L-thyronine on the liver of high fat diet fed rats","authors":"M. Giammanco, S. Aiello, A. Casuccio, M. Guardia, L. Cicero, R. Puleio, I. Vazzana, G. Tomasello, G. Cassata, G. Leto, D. Majo","doi":"10.4081/JBR.2016.5667","DOIUrl":"https://doi.org/10.4081/JBR.2016.5667","url":null,"abstract":"Experimental studies have highlighted that the administration of 3,5-diiodo-L-thyronine (T2) to rats fed diets rich in lipids induces a decrease of cholesterol and triglycerides plasma levels and body weight (BW) without inducing liver steatosis. On the basis of these observations we carried out some experimental in vivo studies to assess the effects of multiple high doses of T2 on the pituitary thyroid axis of rats fed diet rich in lipids. Fifteen male Wistar rats were divided into three groups of five animals each. The first group (N group) received standard diet, the second group was fed with a high fat diet (HFD group), while the third group (HFDT2 group) was additionally given T2 intraperitoneally at a dose level of 70 µg/100 g of BW three times a week up to four weeks. At the end of the treatment, blood sample from each animal was collected, centrifuged and the serum was stored at -20°C. The serum concentrations of thyroidstimulating hormone (TSH), triiodothyronine, thyroxine, adrenocorticotropic hormone, triglycerides, cholesterol, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were then determined. In addition, liver of rats was examined by histology in order to assess the presence and degree of steatosis. The administration of T2 to rats fed with a high fat diet suppressed TSH secretion (P=0.013) while no steatosis was observed in the liver of these animals. Our data show that multiple administrations of high doses of T2 to rats fed diets rich in lipid inhibit TSH secretion and prevent the onset of liver steatosis in these animals.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2016-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2016.5667","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Venturella, M. Tumbarello, L. Navarra, S. Martorana
Micafungin is an antimycotic drug and represents an important addition to the available therapies for the treatment of systemic fungal infections. Micafungin is used: in the treatment of invasive candidiasis, oesophageal and prophylaxis of Candida infections. It inhibits, in a non-competitive way, the synthesis of 1,3-β-D-glucan, a component of fungal cell wall and is rapidly distributed into the tissues. It has a high-rate respectful bond with plasma protein, which is independent from the concentration of the drug. It is metabolized through the liver, being not subject to intense metabolic transformations until the excretion. There is no evidence of systemic accumulation after repeated use. The steady-state is reached in 4-5 days. Medical records examined at the pharmacy of Marsala Hospital highlight that, from 01/06/2014 to 01/08/2014, in this hospital 12 vials were used by the hospitalized patients in the Department of Intensive Care: 8 patients between 75 and 83 years old had a body weight (BW) higher than 40 kg; 3 patients between 40 and 60 years of age had a BW higher than 40 kg, and one 17 year-old patient had a BW of 40 kg. Two patients needed a dose increase, while for the other 10 patients the first dose resulted sufficient. Mycamine ® was used for the treatment of hypovolemic post-operative shock. The most recorded adverse reactions were anemia, hypokalemia, hypomagnesemia, phlebitis, nausea, liver problems. Given the different weight of the subjects, the dosage was different.
Micafungin是一种抗真菌药物，代表了治疗全身性真菌感染的重要补充疗法。米卡芬用于治疗侵袭性念珠菌病、食道和预防念珠菌感染。它以非竞争性的方式抑制1,3-β- d -葡聚糖的合成，葡聚糖是真菌细胞壁的一种成分，并迅速分布到组织中。它与血浆蛋白有高速率的相互尊重的结合，这与药物的浓度无关。它通过肝脏代谢，不受强烈的代谢转化，直到排泄。反复使用后无系统性积累的证据。4-5天达到稳态。在Marsala医院药房检查的医疗记录显示，从2014年6月1日至2014年8月1日，该院重症监护部住院患者使用了12个小瓶:75至83岁的8例患者体重(BW)高于40 kg;3例40 ~ 60岁患者体重大于40 kg, 1例17岁患者体重为40 kg。两名患者需要增加剂量，而其他10名患者第一次剂量就足够了。Mycamine®用于治疗低血容量性术后休克。记录最多的不良反应是贫血、低钾血症、低镁血症、静脉炎、恶心、肝脏问题。由于受试者体重不同，剂量也不同。
{"title":"Monitoring and use of antimycotic (micafungin) for systemic use provided by the pharmacy of Marsala Hospital, Italy","authors":"F. Venturella, M. Tumbarello, L. Navarra, S. Martorana","doi":"10.4081/JBR.2016.5113","DOIUrl":"https://doi.org/10.4081/JBR.2016.5113","url":null,"abstract":"Micafungin is an antimycotic drug and represents an important addition to the available therapies for the treatment of systemic fungal infections. Micafungin is used: in the treatment of invasive candidiasis, oesophageal and prophylaxis of Candida infections. It inhibits, in a non-competitive way, the synthesis of 1,3-β-D-glucan, a component of fungal cell wall and is rapidly distributed into the tissues. It has a high-rate respectful bond with plasma protein, which is independent from the concentration of the drug. It is metabolized through the liver, being not subject to intense metabolic transformations until the excretion. There is no evidence of systemic accumulation after repeated use. The steady-state is reached in 4-5 days. Medical records examined at the pharmacy of Marsala Hospital highlight that, from 01/06/2014 to 01/08/2014, in this hospital 12 vials were used by the hospitalized patients in the Department of Intensive Care: 8 patients between 75 and 83 years old had a body weight (BW) higher than 40 kg; 3 patients between 40 and 60 years of age had a BW higher than 40 kg, and one 17 year-old patient had a BW of 40 kg. Two patients needed a dose increase, while for the other 10 patients the first dose resulted sufficient. Mycamine ® was used for the treatment of hypovolemic post-operative shock. The most recorded adverse reactions were anemia, hypokalemia, hypomagnesemia, phlebitis, nausea, liver problems. Given the different weight of the subjects, the dosage was different.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2016-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2016.5113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-29DOI: 10.1186/s40709-016-0041-z
S. Onyango, H. Roderick, Jaindra Nath Tripathi, R. Collins, H. Atkinson, Richard O. Oduor, L. Tripathi
{"title":"The ZmRCP-1 promoter of maize provides root tip specific expression of transgenes in plantain","authors":"S. Onyango, H. Roderick, Jaindra Nath Tripathi, R. Collins, H. Atkinson, Richard O. Oduor, L. Tripathi","doi":"10.1186/s40709-016-0041-z","DOIUrl":"https://doi.org/10.1186/s40709-016-0041-z","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2016-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40709-016-0041-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65745355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-12DOI: 10.1186/s40709-016-0040-0
M. Dolch, Silke Janitza, A. Boulesteix, Carola Graßmann-Lichtenauer, S. Praun, W. Denzer, G. Schelling, S. Schubert
{"title":"Gram-negative and -positive bacteria differentiation in blood culture samples by headspace volatile compound analysis","authors":"M. Dolch, Silke Janitza, A. Boulesteix, Carola Graßmann-Lichtenauer, S. Praun, W. Denzer, G. Schelling, S. Schubert","doi":"10.1186/s40709-016-0040-0","DOIUrl":"https://doi.org/10.1186/s40709-016-0040-0","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40709-016-0040-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65745344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-03-01DOI: 10.1186/s40709-016-0039-6
A. Kargiotidou, D. Vlachostergios, C. Tzantarmas, I. Mylonas, C. Foti, G. Menexes, A. Polidoros, I. Tokatlidis
{"title":"Addressing huge spatial heterogeneity induced by virus infections in lentil breeding trials","authors":"A. Kargiotidou, D. Vlachostergios, C. Tzantarmas, I. Mylonas, C. Foti, G. Menexes, A. Polidoros, I. Tokatlidis","doi":"10.1186/s40709-016-0039-6","DOIUrl":"https://doi.org/10.1186/s40709-016-0039-6","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40709-016-0039-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65745328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-02-29DOI: 10.1186/s40709-016-0038-7
Guixian Nan, Yan Zhang, Song Li, Imshik Lee, T. Takano, Shenkui Liu
{"title":"NaCl stress-induced transcriptomics analysis of Salix linearistipularis (syn. Salix mongolica)","authors":"Guixian Nan, Yan Zhang, Song Li, Imshik Lee, T. Takano, Shenkui Liu","doi":"10.1186/s40709-016-0038-7","DOIUrl":"https://doi.org/10.1186/s40709-016-0038-7","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2016-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40709-016-0038-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65745319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Guardone, M. Guarducci, L. Gasperetti, F. Susini, Martina Longo, A. Maneschi, Nadav Davidovich, A. Guidi, A. Armani
{"title":"Climate change and food safety: citizen science for monitoring the presence of toxic alien fish species in Italian waters","authors":"L. Guardone, M. Guarducci, L. Gasperetti, F. Susini, Martina Longo, A. Maneschi, Nadav Davidovich, A. Guidi, A. Armani","doi":"10.4081/jbr.2016.6432","DOIUrl":"https://doi.org/10.4081/jbr.2016.6432","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/jbr.2016.6432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The authors report on six cases of gluten-sensitivity, also defined non-celiac gluten sensitivity, characterized by abdominal features (diarrhea, bloating, pain), genetic positivity for predisposition to celiac disease (DQB1* 02 in all cases; DQA1*05 in three; DQA1*02 in two, DQB1*03 in two), negative anti-t-Transglutaminase antibodies, normal mucosa on biopsy in four cases, type 1 of Marsh in one case. The subjects presented frequent central nervous system (CNS) symptoms: headache in three patients, somnolence in one, electroencephalogram aspecific alterations in three (in two of them with previous seizures), leptomeningeal cyst in one, intracranial calcification in one, cerebral gliosis in two. After a gluten-free diet, all intestinal and clinical CNS features remitted, but re-appeared after gluten reintroduction. On the basis of the neurological signs, the authors stress the relevance of immune innate system in the pathogenesis of these cases with possible subsequent evolution on immune adaptive system involvement.
{"title":"Gluten sensitivity and neurological manifestations","authors":"A. Berio, G. Mangiante, G. Mariottini, A. Piazzi","doi":"10.4081/JBR.2015.5405","DOIUrl":"https://doi.org/10.4081/JBR.2015.5405","url":null,"abstract":"The authors report on six cases of gluten-sensitivity, also defined non-celiac gluten sensitivity, characterized by abdominal features (diarrhea, bloating, pain), genetic positivity for predisposition to celiac disease (DQB1* 02 in all cases; DQA1*05 in three; DQA1*02 in two, DQB1*03 in two), negative anti-t-Transglutaminase antibodies, normal mucosa on biopsy in four cases, type 1 of Marsh in one case. The subjects presented frequent central nervous system (CNS) symptoms: headache in three patients, somnolence in one, electroencephalogram aspecific alterations in three (in two of them with previous seizures), leptomeningeal cyst in one, intracranial calcification in one, cerebral gliosis in two. After a gluten-free diet, all intestinal and clinical CNS features remitted, but re-appeared after gluten reintroduction. On the basis of the neurological signs, the authors stress the relevance of immune innate system in the pathogenesis of these cases with possible subsequent evolution on immune adaptive system involvement.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2015.5405","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70233030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-01DOI: 10.1186/s40709-015-0037-0
E. Rouka, D. Kyriakou
{"title":"Identification and functional analysis of the BIM interactome; new clues on its possible involvement in Epstein–Barr Virus-associated diseases","authors":"E. Rouka, D. Kyriakou","doi":"10.1186/s40709-015-0037-0","DOIUrl":"https://doi.org/10.1186/s40709-015-0037-0","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40709-015-0037-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65745307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-11-19DOI: 10.1186/s40709-015-0036-1
Mercedes Pérez-Heredia, J. Clavero-González, Leticia Marchena-Rodríguez
{"title":"Use of melatonin in oral health and as dental premedication","authors":"Mercedes Pérez-Heredia, J. Clavero-González, Leticia Marchena-Rodríguez","doi":"10.1186/s40709-015-0036-1","DOIUrl":"https://doi.org/10.1186/s40709-015-0036-1","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s40709-015-0036-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65745295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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