E. D. Cairano, S. Moretti, F. Daniele, V. Sacchi, C. Perego
The aim of this study was to isolate and characterize rat Adipose Derived Mesenchymal Stem Cells (AD-MSCs) in order to evaluate their proliferative potential and their ability to differentiate in different cell types. AD-MSCs and Derived Mesen- chymal Stem Cells (BM-MSCs) have the same characteristics in terms of plasticity. The advantage of adipose tissue is that it is an easier accessible source and it offers a large amount of MSCs by less invasive surgical tecniques. MSCs were obtained from subcutaneous adipose tissue of Wistar rats. First of all microbiological controls were made to exclude the presence of bacteria or fungi in the tissue. Adipose tissue was mechanically and enzimatically fragmented and stomal cell fraction was seeded in adherent culture flasks in DMEM 20% FBS. After 48h the medium was replaced. Cells were characterized by evaluating: 1)their ability to adhere to the plastic; 2) the clonogenic potential by Colony Forming Unit (CFU) assay; 3) their ability to differentiate in 3 mesodermal lineages (adipocytes, osteocytes and chondrocytes). AD-MSCs are able to differenti- ate in adipocytes, osteocytes and chondrocytes as confirmed by Oil Red'O staining, von Kossa staining and histological analysis respectively. This first characterization is essential for the second part of our study in which we are planning to use AD-MSCs in vivo to restore renal function after an induced ischemic damage in experimental animals.
{"title":"Total internal reflection fluorescence microscopy (TIRFM) as a powerful tool to follow dynamic events at the cell membrane","authors":"E. D. Cairano, S. Moretti, F. Daniele, V. Sacchi, C. Perego","doi":"10.4081/JBR.2015.5161","DOIUrl":"https://doi.org/10.4081/JBR.2015.5161","url":null,"abstract":"The aim of this study was to isolate and characterize rat Adipose Derived Mesenchymal Stem Cells (AD-MSCs) in order to evaluate their proliferative potential and their ability to differentiate in different cell types. AD-MSCs and Derived Mesen- chymal Stem Cells (BM-MSCs) have the same characteristics in terms of plasticity. The advantage of adipose tissue is that it is an easier accessible source and it offers a large amount of MSCs by less invasive surgical tecniques. MSCs were obtained from subcutaneous adipose tissue of Wistar rats. First of all microbiological controls were made to exclude the presence of bacteria or fungi in the tissue. Adipose tissue was mechanically and enzimatically fragmented and stomal cell fraction was seeded in adherent culture flasks in DMEM 20% FBS. After 48h the medium was replaced. Cells were characterized by evaluating: 1)their ability to adhere to the plastic; 2) the clonogenic potential by Colony Forming Unit (CFU) assay; 3) their ability to differentiate in 3 mesodermal lineages (adipocytes, osteocytes and chondrocytes). AD-MSCs are able to differenti- ate in adipocytes, osteocytes and chondrocytes as confirmed by Oil Red'O staining, von Kossa staining and histological analysis respectively. This first characterization is essential for the second part of our study in which we are planning to use AD-MSCs in vivo to restore renal function after an induced ischemic damage in experimental animals.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2015.5161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Cataldo, G. Russo, D. Cerasola, D. Majo, M. Giammanco, M. Traina
The contribution of fat oxidation to energy production during exercise is influenced by intensity of exercise. The aim of this study was to assess the relationship between the highest value of fat oxidation rate (FATmax) and the oxygen uptake (VO2) in sedentary type 2 diabetes (T2D) patients vs healthy sedentary subjects. Sedentary T2D patients and healthy sedentary subjects were evaluated to a graded exercise test, and oxygen uptake and fat oxidation rate were detected. Data show that in T2D patients fat oxidation rate is not impaired and the positive linear correlation between FATmax and both VO2 and VO2max suggests that even in T2D patients the muscle oxidative capacity might increase in response to aerobic training.
{"title":"Relationship between maximal fat oxidation and oxygen uptake: comparison between type 2 diabetes patients and healthy sedentary subjects","authors":"A. Cataldo, G. Russo, D. Cerasola, D. Majo, M. Giammanco, M. Traina","doi":"10.4081/JBR.2014.2136","DOIUrl":"https://doi.org/10.4081/JBR.2014.2136","url":null,"abstract":"The contribution of fat oxidation to energy production during exercise is influenced by intensity of exercise. The aim of this study was to assess the relationship between the highest value of fat oxidation rate (FATmax) and the oxygen uptake (VO2) in sedentary type 2 diabetes (T2D) patients vs healthy sedentary subjects. Sedentary T2D patients and healthy sedentary subjects were evaluated to a graded exercise test, and oxygen uptake and fat oxidation rate were detected. Data show that in T2D patients fat oxidation rate is not impaired and the positive linear correlation between FATmax and both VO2 and VO2max suggests that even in T2D patients the muscle oxidative capacity might increase in response to aerobic training.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2014.2136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Primate play laughing: a comparison between immature great apes and humans","authors":"Giada Cordoni, E. Palagi","doi":"10.4081/JBR.2012.4147","DOIUrl":"https://doi.org/10.4081/JBR.2012.4147","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2012-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2012.4147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Minozzi, F. Bianchi, W. Pantano, P. Catalano, G. Fornaciari
{"title":"III-Treatment of women in Ancient Rome: contribution of paleopathology to the reconstruction of violence. A case report","authors":"S. Minozzi, F. Bianchi, W. Pantano, P. Catalano, G. Fornaciari","doi":"10.4081/JBR.2012.4129","DOIUrl":"https://doi.org/10.4081/JBR.2012.4129","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2012-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2012.4129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micheletti Cremasco Margherita, Re Alessandra, O. Cristiano
{"title":"Physical Ergonomics Contribution to Health Workers’ Well-Being: a Triage Station Analysis","authors":"Micheletti Cremasco Margherita, Re Alessandra, O. Cristiano","doi":"10.4081/JBR.2012.4140","DOIUrl":"https://doi.org/10.4081/JBR.2012.4140","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2012-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2012.4140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70233072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Interdisciplinary Research and Practice in Ergonomics","authors":"Re Alessandra","doi":"10.4081/4064","DOIUrl":"https://doi.org/10.4081/4064","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/4064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70141730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-06-03DOI: 10.4314/EJBMB.V27I1.43196
Ehab M. M. Ali
Asparaginase (EC 3.5.1.1) activity was determined in non germinating seeds and germinating seeds of five Egyptian cowpea (Vigna unguiculata) cultivars (Kareem 7, Dokki 331, Kafer El-Sheikh 1, Kaha 1 and Fodder). The specific activities of germinating seeds asparaginase in different cultivars were higher than the specific activities of non germinated seeds of these cultivars. Asparaginase was purified from Fodder cultivar germinating seeds ( the highest specific activity) and resolved into three peaks with asparaginase activities by DEAE sepharose, designated by asp I, asp II and asp III. The molecular weight of asp II was 70 kDa for native enzyme using gel filtration. By using SDS-PAGE electrophoresis, asp II had molecular weight about 35 kDa suggesting that a dimeric structure for asp II. AspII had a Km value 1.25 mM for asparagine and a pH optimum at 8.0. Asp II had a temperature optimum and heat stability at 40 o C. The fodder cultivar asp II activity was specific for Lasparagine and did not hydrolyze D-asparagine. It is not specific for L-glutamine. Ni 2+ and Co 2+ had activator effects on asp II but other metals ions had inhibitory effect. Key words: Cowpea , Isoenzymes, Asparagine, Glutamine, Antineoplastic effect.
{"title":"Purification and characterization of Vigna unguiculata cultivar asparaginase.","authors":"Ehab M. M. Ali","doi":"10.4314/EJBMB.V27I1.43196","DOIUrl":"https://doi.org/10.4314/EJBMB.V27I1.43196","url":null,"abstract":"Asparaginase (EC 3.5.1.1) activity was determined in non germinating seeds and germinating seeds of five Egyptian cowpea (Vigna unguiculata) cultivars (Kareem 7, Dokki 331, Kafer El-Sheikh 1, Kaha 1 and Fodder). The specific activities of germinating seeds asparaginase in different cultivars were higher than the specific activities of non germinated seeds of these cultivars. Asparaginase was purified from Fodder cultivar germinating seeds ( the highest specific activity) and resolved into three peaks with asparaginase activities by DEAE sepharose, designated by asp I, asp II and asp III. The molecular weight of asp II was 70 kDa for native enzyme using gel filtration. By using SDS-PAGE electrophoresis, asp II had molecular weight about 35 kDa suggesting that a dimeric structure for asp II. AspII had a Km value 1.25 mM for asparagine and a pH optimum at 8.0. Asp II had a temperature optimum and heat stability at 40 o C. The fodder cultivar asp II activity was specific for Lasparagine and did not hydrolyze D-asparagine. It is not specific for L-glutamine. Ni 2+ and Co 2+ had activator effects on asp II but other metals ions had inhibitory effect. Key words: Cowpea , Isoenzymes, Asparagine, Glutamine, Antineoplastic effect.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2009-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70527140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Tumminello, M. Crescimanno, C. Flandina, G. Leto, M. Guardia, M. Giammanco, D. Majo, E. Tripoli, M. Sepporta, M. Tolomeo
The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 ug/ml and 32 ug/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce diffentiation on this cell line.
{"title":"Effect of extra virgin olive oli phenols on HL60 cell lines sensitive and resistant to anthracyclines","authors":"F. Tumminello, M. Crescimanno, C. Flandina, G. Leto, M. Guardia, M. Giammanco, D. Majo, E. Tripoli, M. Sepporta, M. Tolomeo","doi":"10.4081/JBR.2009.4729","DOIUrl":"https://doi.org/10.4081/JBR.2009.4729","url":null,"abstract":"The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 ug/ml and 32 ug/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce diffentiation on this cell line.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2009-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2009.4729","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study is an analisis of seroproteins conducted on blood samples of subjects living in mountain communities of the French Western Alps. In particular, the third component of complement (C3), the group specific component (Gc), properdin B factor (Bf), haptoglobin (Hp) and transferrin (Tf) were considered, these being very important markers in human genetics research. The data for these systems are the first results of the biological study of populations of the Western Alps. They add to our otherwise scanty knowledge about the distribution of these polymorphisms in the populations under study. The results were compared with those in the literature on European populations, in order to identify origins and microevolutionary processes, as well as biotransformations related to environmental adaptation.
{"title":"Polymorphism of seroproteins in communities of the Briançon region: comparison with European populations","authors":"N. Salis, E. R. Massa","doi":"10.4081/JBR.2009.4735","DOIUrl":"https://doi.org/10.4081/JBR.2009.4735","url":null,"abstract":"The present study is an analisis of seroproteins conducted on blood samples of subjects living in mountain communities of the French Western Alps. In particular, the third component of complement (C3), the group specific component (Gc), properdin B factor (Bf), haptoglobin (Hp) and transferrin (Tf) were considered, these being very important markers in human genetics research. The data for these systems are the first results of the biological study of populations of the Western Alps. They add to our otherwise scanty knowledge about the distribution of these polymorphisms in the populations under study. The results were compared with those in the literature on European populations, in order to identify origins and microevolutionary processes, as well as biotransformations related to environmental adaptation.","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2009-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JBR.2009.4735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mora, L. Mastracci, F. Grillo, G. Gaggero, E. Fulcheri
{"title":"CdX2 expression in ovarian teratomatous cells with intestinal differentiation.","authors":"M. Mora, L. Mastracci, F. Grillo, G. Gaggero, E. Fulcheri","doi":"10.4081/JBR.2006.8074","DOIUrl":"https://doi.org/10.4081/JBR.2006.8074","url":null,"abstract":"","PeriodicalId":42615,"journal":{"name":"Journal of Biological Research-Bollettino della Societa Italiana di Biologia Sperimentale","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2006-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70232259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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