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Effects of water depth on digestive enzymes activities of Clarias gariepinus (Burchell, 1822) fingerlings 水深对加里平Clarias gariepinus (Burchell, 1822)鱼种消化酶活性的影响
Pub Date : 2018-11-27 DOI: 10.4314/TZOOL.V16I1.5
K. Salisu, B. Suleiman, S. Abdullahi
The effects of water depth on the digestive enzymes activities of Clarias gariepinus was investigated. Ten concrete tanks (50x150x100 cm capacity) were used; each tank was filled with water at different experimental water depths (25, 50, 75,100 and 125 cm) under static culture system. The activity of protease, lipase and amylase from the pancreas of Clarias gariepinus was determined. Digestive protease, lipase and amylase showed optimum activities at 125 cm water depth with amylase activity showing greater variation across treatments compared to the other two digestive enzymes. Water depth positively influenced the release of these enzymes for feed digestion to occur with lipase being the most influenced by water depth. In conclusion water depth affects digestive enzymes activities in Clarias gariepinus fingerlings and it is recommended that 125 cm water depth should be used in culturing Clarias gariepinus.Keywords: Amylase; Clarias gariepinus; lipase; protease; water depth.
研究了不同水深对加里平Clarias gariepinus消化酶活性的影响。混凝土储罐10个,容量为50x150 × 100 cm;在静态培养系统下,每个水箱分别充入不同实验水深(25、50、75、100和125 cm)的水。测定了加里宾Clarias gariepinus胰腺蛋白酶、脂肪酶和淀粉酶的活性。消化酶、脂肪酶和淀粉酶在125 cm水深处表现出最佳活性,淀粉酶活性在不同处理间差异较大。水深正影响这些酶在饲料消化中的释放,其中脂肪酶受水深影响最大。综上所述,水深对中国克拉尾鱼鱼种消化酶活性有影响,建议养殖深度为125 cm。关键词:淀粉酶;claria gariepinus;脂肪酶;蛋白酶;水的深度。
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引用次数: 0
Insecticidal activities of three weed varieties against the Bamboo Powder Post Beetle Dinoderus minutus Fabricius (Coleoptera: Bostrichidae) 3个杂草品种对竹粉后甲虫的杀虫活性研究(鞘翅目:蝽科)
Pub Date : 2018-11-27 DOI: 10.4314/tzool.v16i1.6
K. Popoola, J. A. George-Onaho, O. T. Alamu, A. E. Ayandokun
The insecticidal activities of crude ethanol extracts of leaves of three weed species, Siam weed, Chromolaena odorata Linn., Milk weed, Euphorbia heterophylla Linn. and Tree Marigold Tithonia diversifolia (Hemsl.) A, Gray on the insect, Dinoderus minutus Fabricius, were investigated on bamboo as substrate. Bamboo blocks were treated with different concentrations: control (positive), control (negative), 20% and 25% of each plant extracts. Twenty-five active adult D. minutus insects were introduced into each setup of five bamboo blocks in 0.32 L jars for 60 days and were replicated four times in a Completely Randomized design in the laboratory. Data were collected on mortality of D. minutus at 5, 10 and 15 days after introduction. The number of holes bored by D. minutus on bamboo blocks after 60 days of storage were counted and the percentage weight loss of bamboo block was calculated using standard procedure. The result showed that the highest and the lowest percentage mortality of D. minutus were recorded in 25% T. diversifolia (94.67±2.67) and control (–) respectively at 15 days after treatment. The number of holes bored by D. minutus on bamboo blocks in the control treatments was higher than the number of holes bored on bamboo blocks treated with different concentrations of the three extracts. The mean percentage weight loss of bamboo blocks obtained at 20% and 25% concentrations of each extract was lower than that of the two control treatments. The maximum wood protection (1.33±0.22) against D. minutes was recorded at 25% concentration of T. diversifolia. The number of D. minutus holes on bamboo block correlated positively (R 2 = 0.538; p<0.0001) with the percentage weight loss of bamboo. The three weeds at 20% and 25% concentrations could be used in the management of D. minutus on bamboo. Keywords : Plant extract; Dinoderus minutus ; bamboo pest; mortality; weight loss.
对暹罗草、臭毛草三种杂草叶片粗乙醇提取物的杀虫活性进行了研究。,乳草,大戟。万寿菊(Tithonia difolia)A、以竹材为底物,对灰翅昆虫Dinoderus minuus Fabricius进行了研究。竹块分别用不同浓度的植物提取物处理:对照(阳性)、对照(阴性)、20%和25%。采用完全随机实验设计,将25只活性小夜蛾成虫放入5个竹块中,每组放置60 d,重复饲养4次。收集了引种后5、10和15 d的死亡率数据。采用标准程序计算贮藏60 d后,竹块上的刺孔数和竹块失重率。结果表明:处理15 d后,25%分叶散叶菊和对照分叶散叶菊死亡率最高(94.67±2.67),最低(-);3种提取物不同浓度处理竹块上的钻孔数均高于对照处理竹块上的钻孔数。两种提取物浓度分别为20%和25%时,竹块的平均失重率均低于对照处理。在25%浓度下,百叶松对木蠹的保护效果最大(1.33±0.22)。竹块上的D. minutus孔洞数正相关(r2 = 0.538;P <0.0001),与竹材失重率呈正相关。三种杂草浓度分别为20%和25%时,可用于竹材上的短叶枯病管理。关键词:植物提取物;分钟恐龙;竹子害虫;死亡率;减肥。
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引用次数: 0
Effects of artemether on parasite burden and liver pathology in mice infected with Ibadan isolate of Schistosoma mansoni 蒿甲醚对感染曼氏血吸虫伊巴丹分离株小鼠寄生虫负荷和肝脏病理的影响
Pub Date : 2018-11-27 DOI: 10.4314/tzool.v16i1.8
F. Adejoro, E. Ilo, O. G. Ohore, A. Odaibo
This study aimed at evaluating the pathological features associated with Schistosoma mansoni infection and assessment of the efficacy of artemether in the treatment of intestinal schistosomiasis in laboratory mice. Cercariae harvested from naturally infected Biomphalaria pfeifferi obtained from Odo Ona River in Ibadan, Oyo State, Nigeria, were used to infect Swiss albino mice in the laboratory. A total of 50 mice were divided into 5 groups and artemether (300mg/kg) was administered to two of the groups at 15th and 42nd day post-infection respectively, Praziquantel (600mg/kg) was administered to a group as the control drug and another group was used as the infected control group while the last group was uninfected. Mice were sacrificed and perfused at 8th week post-infection for worm recovery, examination of the visceral organs and histopathology were conducted on liver and spleen of infected and uninfected groups. Schistosoma mansoni isolate from Ibadan, caused severe liver pathology in mice, and thus presents features indicative of epidemiological importance in human schistosomiasis. Worm burden reduction (98%) was observed in mice treated at day 15 post-infection with artemether (300 mg/kg) while 77% worm reduction was observed in the group of mice treated at day 42 post infection with artemether (300 mg/kg). Granulomas were not observed in the liver of mice treated at day 15 post-infection while those of untreated group, which had multiple granulomas. Inflammatory cellular infiltrations were observed in liver of infected mice however, the mice treated at day 15 post-infection had mild infiltration likely due to fewer eggs present in the liver. Praziquantel used as the routine control drug was less effective compared to artemether. Worm reduction (53%) was recorded for mice treated with praziquantel, gross and histopathology also revealed eggs diffusely deposited in the liver of the mice. Artemether, a derivative of artemisinin, showed a good antischistosomal effect that could be an alternative and/or complement praziquantel in the control of schistosomiasis in endemic areas. Keywords : Artemether; Schistosoma mansoni; Ibadan isolate; Swiss albino mice; gross pathology; histopathology.
本研究旨在评估与曼氏血吸虫感染相关的病理特征,并评估蒿甲醚治疗实验室小鼠肠道血吸虫病的疗效。从尼日利亚奥约州伊巴丹市奥多奥纳河自然感染的普费费生物phalaria采集尾蚴,用于实验室感染瑞士白化小鼠。将50只小鼠分为5组,感染后第15天和第42天分别给药蒿甲醚(300mg/kg),吡喹酮(600mg/kg)作为对照组,另一组作为感染对照组,最后一组不感染。感染后第8周处死小鼠灌流虫虫恢复,对感染组和未感染组的肝脏、脾脏进行脏器检查和组织病理学检查。来自伊巴丹的曼氏血吸虫分离物在小鼠中引起严重的肝脏病理,因此显示出在人类血吸虫病中具有流行病学重要性的特征。感染后第15天用蒿甲醚(300 mg/kg)治疗的小鼠虫量减少98%,感染后第42天用蒿甲醚(300 mg/kg)治疗的小鼠虫量减少77%。感染后第15天,治疗组小鼠肝脏未见肉芽肿,而未治疗组小鼠肝脏出现多发肉芽肿。在感染小鼠的肝脏中观察到炎症细胞浸润,然而,感染后第15天处理的小鼠有轻微的浸润,可能是由于肝脏中存在的卵子较少。吡喹酮作为常规对照药物与蒿甲醚相比效果较差。经吡喹酮处理的小鼠蛔虫减少(53%),大体和组织病理学也显示卵弥漫性沉积在小鼠肝脏中。青蒿素衍生物蒿甲醚显示出良好的抗血吸虫作用,可作为吡喹酮的替代和/或补充,在流行地区控制血吸虫病。关键词:蒿甲醚;曼氏裂体吸虫;伊巴丹隔离;瑞士白化小鼠;总值病理学;组织病理学。
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引用次数: 1
Estimates of home range sizes of Arvicanthis niloticus Desmarest, 1822 (Muridae) in savannah fields near Kano, Nigeria 奈及利亚卡诺附近萨凡纳田野里1822年niloticus Desmarest (Muridae)家栖范围的估计
Pub Date : 2018-11-27 DOI: 10.4314/TZOOL.V16I1.7
S. Rabiu, R. K. Rose
Determination of rodent home range (HR) is invaluable for characterizing population distribution and strategies for accessing food, mate and nest sites, as well as, dispersal into new fields. The present study, in savannah fallow fields near Kano, Nigeria, was aimed at estimating HR sizes of Arvicanthis niloticus , a species widespread in much of Africa. By Capture-Mark-Recapture (CMR) in a five to six-day/month sessions on replicate grids during 1990-1992; and 25 years later followed by a one year, quarterly, appraisal surveillance during 2015-2016, the movement patterns of A. niloticus were analyzed by means of Minimum Convex Polygon (MCP) calculations. With GLM ANOVA, comparisons between sexes, seasons and periods of study were made. Further HR comparisons were made with Kruskal-Wallis tests. Predictors (year of study, sex of the rodent and season) which contributed to significant differences in HR sizes were also identified with stepwise regression. Overall, ANOVA results showed that sex had significant effect (p<0.05) on HR, larger (1,036 m 2 ) mean HR sizes than that of females (850 m 2 ). Seasonal differences were also significant (p<0.05). Kruskal-Wallis tests also indicated significant (p<0.05) differences between sexes, seasons and age groups, being higher in males than females; greater in dry than in rainy season; but marginally greater in adults than juveniles (p=0.056). Generally, HR size values for 2015-2016, the second segment of the study, had shown similar patterns as those of the 1990s, again with significant (p<0.05) differences between sexes, seasons, and between sexes within seasons. However, overall HR values in 2015-2016 were lower than those of 1990-1992 composite year. From the forgoing, we may conclude that though long-term temporal lapse and ensuing environmental degradation may reduce HR sizes in A. niloticus , the general patterns of age-sex and seasonally related differences do not change. Keywords : Africa; grassfields; home range; Nile rat; movement; rodents.
确定啮齿动物的活动范围(HR)对于确定种群分布特征和获取食物、配偶和筑巢地点以及向新领域扩散的策略具有重要意义。目前的研究在尼日利亚卡诺附近的大草原休耕地进行,目的是估计在非洲大部分地区广泛分布的阿维坎斯尼罗ticus的HR大小。通过捕获-标记-再捕获(CMR),在1990-1992年期间在复制网格上进行5至6天/月的会议;25年后,采用最小凸多边形(Minimum Convex Polygon, MCP)方法,在2015-2016年进行1年季度评价监测,分析niloticus的移动模式。通过GLM方差分析,对性别、季节和研究期间进行了比较。进一步的人力资源比较采用Kruskal-Wallis测试。预测因素(研究年份、啮齿动物性别和季节)也通过逐步回归确定了HR大小的显著差异。总体而言,方差分析结果显示,性别对HR有显著影响(p<0.05),平均HR大小(1,036 m2)大于雌性(850 m2)。季节差异也显著(p<0.05)。Kruskal-Wallis检验也显示性别、季节和年龄组之间存在显著差异(p<0.05),男性高于女性;旱季比雨季更大;但在成人中略高于青少年(p=0.056)。总体而言,2015-2016年(研究的第二部分)的HR大小值与20世纪90年代的模式相似,性别之间、季节之间以及季节内性别之间的差异也显著(p<0.05)。但2015-2016年整体人力资源值低于1990-1992年复合年。综上所述,我们可以得出结论,尽管长期的时间流逝和随之而来的环境退化可能会减少尼罗蠓的HR大小,但年龄-性别和季节相关差异的一般模式并没有改变。关键词:非洲;grassfields;活动范围;尼罗河老鼠;运动;啮齿动物。
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引用次数: 0
Cryptosporidium infection among children attending some hospitals in Funtua Local Government Area, Katsina State, Nigeria 在尼日利亚卡齐纳州富图阿地方政府区一些医院就诊的儿童中的隐孢子虫感染
Pub Date : 2018-11-27 DOI: 10.4314/TZOOL.V16I1.1
A. Agba, T. Aken'Ova, P. Audu
Four hundred stool samples (76 watery and 324 soft-formed) obtained from children attending three hospitals in Funtua Local Government Area of Katsina State were examined for Cryptosporidium oocysts. A structured questionnaire was administered to determine the factors that enhance the prevalence of infection. Stool samples were examined for Cryptosporidium oocysts using formol-ether concentration technique and the modified Ziehl Neelson staining technique. The overall prevalence of infection obtained was 4%. The results showed that 10.5% watery stools and 2.5% soft-formed stools contained Cryptosporidium oocysts (p 0.05). More male children were infected (4.9%) than females (3.2%), but the difference was not statistically significant (p>0.05). Infection was more prevalent in children who defaecated in open fields than those who used pit latrines and water closets (p>0.05). This study showed that source of drinking water, eating of food served in the same plate, washing of hands prior to eating, washing of fruits and vegetables before eating, washing of hands after use of toilet, contact with animals as well as other children did not influence Cryptosporidium infection.Keywords: Cryptosporidium; immunocompetent; immunocompromised; microvilli.
从在卡齐纳州富图阿地方政府区三家医院就诊的儿童中采集的400份粪便样本(76份水样,324份软样)进行了隐孢子虫卵囊检查。通过一份结构化的问卷来确定提高感染流行率的因素。采用甲醚浓度法和改良Ziehl - Neelson染色法检测粪便标本隐孢子虫卵囊。获得的感染总流行率为4%。结果显示,10.5%的水样便和2.5%的软质便含有隐孢子虫卵囊(p < 0.05)。男童感染率(4.9%)高于女童感染率(3.2%),差异无统计学意义(p < 0.05)。在露天场所排便的儿童感染发生率高于使用坑式厕所和厕所的儿童(p < 0.05)。这项研究表明,饮用水源、吃同一盘子里的食物、吃饭前洗手、吃饭前洗水果和蔬菜、上厕所后洗手、与动物接触以及与其他儿童接触对隐孢子虫感染没有影响。关键词:隐孢子虫;免疫活性的;免疫功能不全的;微绒毛。
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引用次数: 0
Development and appraisal of new biodiversity loss and conservation game (BLACOG) in assessing students’ knowledge, attitude and perception of biodiversity conservation 新生物多样性丧失与保护游戏(BLACOG)的开发与评价,以评估学生对生物多样性保护的知识、态度和认知
Pub Date : 2018-11-27 DOI: 10.4314/tzool.v16i1.2
A. Aladesida, G. Dedeke, E. O. Onifade, J. Bamidele, U. Ekpo
Educational programmes have for long been used in biodiversity conservation; however introduction of board games in these efforts is a new concept, which is believed, will result in greater achievements. This study developed and used a new educational board game, biodiversity loss and conservation game (BLACOG) and a test-questionnaire to appraise the knowledge, attitude and perception of biodiversity (KAPOB) among secondary school students in Abeokuta, Nigeria. Students (n=283) were selected randomly from three schools, two of the schools serving as Experimental Groups while the third served as the control-group. The test-questionnaire, rated on a Likert scale, was administered to both the control and experimental groups prior to and after five exposures to the game, though the control-group did not have any exposure to the game. Data obtained were tested for significant differences in the mean scores of the respondents in the tests prior to and after the game. T-test comparison of the mean scores of the pre- and post-tests of the Control Group was not significantly different (p=0.207). The mean scores in the post-test were significantly higher than the pre-test scores in both of the experimental schools. The results also showed that, though the mean scores of the pre-tests of the control and experimental groups were not significantly different, the mean score in the post-test of the Experimental Group was significantly higher than in the control group. This study showed that the use of BLACOG had significantly positive effects on the KAPOB of the experimental group and that the game could become a veritable educational tool for teaching biodiversity conservation in schools.Keywords: Conservation education; biodiversity conservation; biodiversity loss; biodiversity education game.
长期以来,教育方案一直用于生物多样性保护;然而,在这些努力中引入桌游是一个新概念,相信会取得更大的成就。本研究开发并使用一种新的教育棋盘游戏——生物多样性丧失和保护游戏(BLACOG)和一份测试问卷来评估尼日利亚阿贝奥库塔中学生对生物多样性的知识、态度和感知。从三所学校随机抽取学生283人,其中两所学校作为实验组,另一所学校作为对照组。测试问卷采用李克特量表(Likert scale)进行评分,在五次接触游戏之前和之后分别对对照组和实验组进行测试,但对照组没有任何接触游戏的经历。对获得的数据进行了测试,以确定在游戏之前和之后的测试中应答者的平均分数是否存在显著差异。对照组前后检验均分的t检验比较差异无统计学意义(p=0.207)。两所实验学校的后测平均分均显著高于前测平均分。结果还显示,虽然对照组和实验组的前测均分差异不显著,但实验组的后测均分显著高于对照组。本研究表明,使用BLACOG对实验组的KAPOB有显著的正向影响,该游戏可以成为学校生物多样性保护教学的名副其实的教育工具。关键词:保护教育;生物多样性保护;生物多样性的丧失;生物多样性教育游戏。
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引用次数: 0
Morphometric characteristics and condition factor of Chrysichthys nigrodigitatus (Lacepede, 1803) and Sarotherodon melanotheron (Ruppell, 1852) in the Lagos Lagoon 拉各斯泻湖黑digitatus Chrysichthys (Lacepede, 1803)和Sarotherodon melanotheron (Ruppell, 1852)的形态特征和条件因子
Pub Date : 2018-11-27 DOI: 10.4314/tzool.v16i1.4
B. Emmanuel, O. Akinniyi, E. G. Inegbedion
One hundred and fifty (150) specimens of Sarotherodon melanotheron and 150 specimens of Chrysichthys nigrodigitatus were collected from Better-life Fish Market in Makoko area of Lagos State between June-August 2016 to provide information on the size distribution, length-weight relationship, determine regression equation and coefficient of determination of morphometric parameters and condition factor of the species. Size ranges of 10-22 cm and 15-33 cm were observed in S. melanotheron (20-158 g) and C. nigrodigitatus (30-279 g) respectively. Length-weight relationship of S. melanotheron and C. nigrodigitatus were described by the equation Log W = -1.107 + 2.4478 Log L and Log W = -1.9287 + 2.8484 Log L respectively. The b values (growth exponent), 2.4478 and 2.8484 for body weight on total length of S. melanotheron and C. nigrodigitatus respectively indicate negative allometric growth which implies that the fishes become slenderer as they increase in weight. The least coefficient of determination ( r 2 ), 0.4305 ( S. melanotheron ) and 0.2221 ( C. nigrodigitatus ) were obtained in snout length on total length and pelvic fin length on total length respectively. High values coefficient of determination, obtained indicates a high degree of positive correlation between the different morphometric parameters with the reference length (total length). All mean condition factors ( k ) calculated for species, size classes and sexes are all above 1 and this reveals that they are all in good conditions and that the environment is suitable for both species with regards to the feeding condition. This study provides some biological information on the condition of the species in the Lagos Lagoon as at the period of the study. Keywords : Morphometric characteristics; condition factor; Chrysichthys nigrodigitatus ; Sarotherodon melanotheron ; Lagos Lagoon.
2016年6 - 8月,在拉各斯州Makoko地区的好生活鱼市场采集了150只(150只)黑颈沙齿龙(Sarotherodon melanotheron)和150只黑齿蝶(Chrysichthys nigrodigitatus)标本,分析了该物种的体型分布、长重关系,确定了回归方程和形态计量参数和条件因子的决定系数。黑皮棘鱼(20 ~ 158 g)和黑趾棘鱼(30 ~ 279 g)的体长范围分别为10 ~ 22 cm和15 ~ 33 cm。S. melanotheron和C. nigrodigitatus的长权关系分别为Log W = -1.107 + 2.4478 Log L和Log W = -1.9287 + 2.8484 Log L。黑颡鱼(S. melanotheron)和黑颡鱼(C. nigrodigitatus)的体重/总长度的b值分别为2.4478和2.8484,表明其呈负异速生长,即随着体重的增加而变细。鼻部长度对总长度的决定系数最小,为0.4305 (S. melanotheron),盆鳍长度对总长度的决定系数最小,为0.2221 (C. nigrodigitatus)。得到的高值决定系数表明,不同形态计量参数与参考长度(总长度)之间存在高度正相关。计算出的物种、大小类别和性别的平均条件因子(k)均大于1,表明它们都处于良好的条件,并且在摄食条件方面环境适合两种物种。本研究提供了研究期间拉各斯泻湖物种状况的一些生物学信息。关键词:形态计量学特征;条件因素;黑趾蝶;黑齿龙;拉各斯泻湖。
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