Emilia coccinea enhances the treatment of several disease conditions include; vertigo management, ringworm, cough, gonorrhoea, ulcers, lice, measles, seizure, eye drop. This study investigates the phytochemistry, In-vitro antioxidant, anti-ulcerogenic, microbicidal and biosafety effect of aqueous extracts of E. coccinea using animal model. E. coccinea leaf was freshly obtained, shade dried, pulverized and prepared into aqueous extract. Standard procedure were used for the evaluation of the phytochemicals, in-vitro antioxidant and antimicrobial activities. Twenty five (25) Wistar rats were acclimitized and randomly selected into five groups (n=5) such as untreated group, 10 mg/kg cimetidine and graded doses (100, 200 and 400 mg/kg b.w.) of aqueous extract of E. coccinea to evaluate antiulcer and biosafety effects. Results from the qualitative and quantitative phytochemical screening showed the present of phenol (47.19 mg), saponins (84.64 mg), alkaloids (75.17 mg), cardiac glycosides (63.12 mg) and anthraquinone (48.79 mg). The antioxidant property showed a competitive scavenging effect against DPPH radical when compared with ascorbic acids. Microbial activities of the aqueous extract of E. coccinea at various concentration elicited inhibitory effect against Salmonella typhii, Escherichia coli, Helicobacter pyloris. The ulceration in rat stomach lining induced with 70% ethanol and pretreated with prophylactic measure of aqueous crude extract showed significant reduction in ulcer count, ulcer index with increased % inhibition of ulcer when compared with untreated and reference control. The haematological and histopathological study shows no significant difference in the aqueous crude extract with absent toxicity when comparisons with the control.
{"title":"Phytochemistry, In-vitro antioxidant, Microbicidal, Anti-ulcerogenic and Biosafety Potential of Emilia coccinea Aqueous Extract in Animal models.","authors":"M. Idu, B. Gabriel, Charles O. Agboonogieva","doi":"10.57056/ajb.v2i2.43","DOIUrl":"https://doi.org/10.57056/ajb.v2i2.43","url":null,"abstract":"Emilia coccinea enhances the treatment of several disease conditions include; vertigo management, ringworm, cough, gonorrhoea, ulcers, lice, measles, seizure, eye drop. This study investigates the phytochemistry, In-vitro antioxidant, anti-ulcerogenic, microbicidal and biosafety effect of aqueous extracts of E. coccinea using animal model. E. coccinea leaf was freshly obtained, shade dried, pulverized and prepared into aqueous extract. Standard procedure were used for the evaluation of the phytochemicals, in-vitro antioxidant and antimicrobial activities. Twenty five (25) Wistar rats were acclimitized and randomly selected into five groups (n=5) such as untreated group, 10 mg/kg cimetidine and graded doses (100, 200 and 400 mg/kg b.w.) of aqueous extract of E. coccinea to evaluate antiulcer and biosafety effects. Results from the qualitative and quantitative phytochemical screening showed the present of phenol (47.19 mg), saponins (84.64 mg), alkaloids (75.17 mg), cardiac glycosides (63.12 mg) and anthraquinone (48.79 mg). The antioxidant property showed a competitive scavenging effect against DPPH radical when compared with ascorbic acids. Microbial activities of the aqueous extract of E. coccinea at various concentration elicited inhibitory effect against Salmonella typhii, Escherichia coli, Helicobacter pyloris. The ulceration in rat stomach lining induced with 70% ethanol and pretreated with prophylactic measure of aqueous crude extract showed significant reduction in ulcer count, ulcer index with increased % inhibition of ulcer when compared with untreated and reference control. The haematological and histopathological study shows no significant difference in the aqueous crude extract with absent toxicity when comparisons with the control.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123247230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Notre objectif est de présenter une synthèse sur l’étude de la stabilité du rendement de dix variétés de blé dur expérimentées pendant quatre ans (2009-2013) dans la région semi-aride de Sétif. Nous utilisons au cours de cette étude trois indices de stabilité paramétriques et non paramétriques pour évaluer les performances des dix génotypes testés. L’étude des indices paramétrique de stabilité, notamment l'écovalence de Wricke (Wi²) a montré que les génotypes Mexicali75 (4.23) et Hoggar (11.07) sont considérés comme les plus stables parmi les dix génotypes testés. Alors qu'en termes d'indice de supériorité (Pi), Polonicum (29,61), Sooty (33,99), Altar84 (34,47) et Mexicali75 (35,37) ont les valeurs les plus faibles de (Pi). L'indice d'adaptabilité géométrique (GAI) a montré que les génotypes Mexicali75 (42,00), Kucuk (40,26) et Altar84 (40,03) sont les plus stables. Les indices non paramétriques Si (2), Si (3), Si (6) ont des corrélations non signifiantes avec le rendement moyen en grains. L’étude de la variabilité génotypique du rendement en grains a montré que Mexicali75 était le génotype qui avait la plus petite variation de rendement au cours des quatre saisons. Globalement, Mexicali75, Hoggar et Kucuk sont les génotypes très stables.
{"title":"Etude de la stabilité de quelques variétés de blé dur (triticum durum desf.) dans les conditions semis aride du Sétif","authors":"Ali Guendouz, Abdelmalek Oulmi, Frih Benalia","doi":"10.57056/ajb.v2i1.39","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.39","url":null,"abstract":"Notre objectif est de présenter une synthèse sur l’étude de la stabilité du rendement de dix variétés de blé dur expérimentées pendant quatre ans (2009-2013) dans la région semi-aride de Sétif. Nous utilisons au cours de cette étude trois indices de stabilité paramétriques et non paramétriques pour évaluer les performances des dix génotypes testés. L’étude des indices paramétrique de stabilité, notamment l'écovalence de Wricke (Wi²) a montré que les génotypes Mexicali75 (4.23) et Hoggar (11.07) sont considérés comme les plus stables parmi les dix génotypes testés. Alors qu'en termes d'indice de supériorité (Pi), Polonicum (29,61), Sooty (33,99), Altar84 (34,47) et Mexicali75 (35,37) ont les valeurs les plus faibles de (Pi). L'indice d'adaptabilité géométrique (GAI) a montré que les génotypes Mexicali75 (42,00), Kucuk (40,26) et Altar84 (40,03) sont les plus stables. Les indices non paramétriques Si (2), Si (3), Si (6) ont des corrélations non signifiantes avec le rendement moyen en grains. L’étude de la variabilité génotypique du rendement en grains a montré que Mexicali75 était le génotype qui avait la plus petite variation de rendement au cours des quatre saisons. Globalement, Mexicali75, Hoggar et Kucuk sont les génotypes très stables.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134638630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to screening the phytogenic chemical compounds of the seeds of Chenopodium quinoa, obtained from Wilaya of El-oued. The chemical study showed that the plant contained a number of secondary metabolites, flavonoids, tannins, saponins, steroids and triterpenes, glycosids while the absence of alkaloids and comarin in Chenopodium quinoa. Folin-Ciocalteau colorimetric were used to determine the total phenolic con-tent (TPC), in the hydroalcoholic seeds extracts. The yield of the methanolic extract was estimated at 36.66%. As for the quantitative content of polyphenols, it is 11.647 ± 1.91µg AGE / mg extrait From this, Chenopodium quinoa is considered a nutritional and therapeutic value because it contains secondary metabolites
本研究的目的是筛选产自El-oued Wilaya的藜麦Chenopodium quinoa种子的植物性化合物。化学研究表明，藜麦中含有黄酮类化合物、单宁、皂苷、甾体、三萜、糖苷等次生代谢产物，而不含生物碱和比较素。采用Folin-Ciocalteau比色法测定水醇种子提取物中总酚含量。甲醇提取物得率为36.66%。多酚的定量含量为11.647±1.91µg AGE / mg提取物。由此可见，藜麦藜麦含有次生代谢产物，具有一定的营养和治疗价值
{"title":"Preliminary Phytochemical Screening, Quantification of phenolic compounds, of Plant Extract from Chenopodium quinoa","authors":"Mounira Kadri","doi":"10.57056/ajb.v2i1.40","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.40","url":null,"abstract":"The aim of this study was to screening the phytogenic chemical compounds of the seeds of Chenopodium quinoa, obtained from Wilaya of El-oued. The chemical study showed that the plant contained a number of secondary metabolites, flavonoids, tannins, saponins, steroids and triterpenes, glycosids while the absence of alkaloids and comarin in Chenopodium quinoa. Folin-Ciocalteau colorimetric were used to determine the total phenolic con-tent (TPC), in the hydroalcoholic seeds extracts. The yield of the methanolic extract was estimated at 36.66%. As for the quantitative content of polyphenols, it is 11.647 ± 1.91µg AGE / mg extrait From this, Chenopodium quinoa is considered a nutritional and therapeutic value because it contains secondary metabolites","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130718853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This work is devoted to the study of medicinal plants in the southern region of BordjBouArreridj (communes of the Daïra of El Hammadia), and their different uses in traditional medecine. A series of surveys were conducted in the study area with inhabitants and herbalists, to acquire more informations about the therapeutic uses of medicinal plants practiced by the local population. The analysis of the results allowed us to identify 78 species belonging to 36 families, the most represented are: Apiaceae (13.92%), Lamiaceae (12.65%), Asteraceae (11.39%) and Liliaceae (5.06%). The most commonly parts used from these plants are respectively: the leaves (34.21%), the stems (17.54%), the fruits (12.28%), the roots (10.52%) and the seeds (8.77%). Medicinal plants are prepared and used as powder, infusion, especially as decoction and poultice. The most used is the decoction. The main common health problem treated are; digestive disorders (29.81%), respiratory disorders (10.55%), skin inflammations (9.17%), urinary inflammations (8.25%), liver diseases (6.88%) and neurologic problems (6.42%). El Hammadia has good ethnobotanical potential of medicinal plants. This study is the first contribution to the ethnobotany of this region. We have gathered from this province some considerable knowledge about local medicinal plants for treating diseases.
{"title":"Ethnobotanical survey of herbal remedies traditionally used in El Hammadia (Southern region of the province of Bordj Bou Arreridj, Algeria)","authors":"H. Bendif","doi":"10.57056/ajb.v2i1.34","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.34","url":null,"abstract":"This work is devoted to the study of medicinal plants in the southern region of BordjBouArreridj (communes of the Daïra of El Hammadia), and their different uses in traditional medecine. A series of surveys were conducted in the study area with inhabitants and herbalists, to acquire more informations about the therapeutic uses of medicinal plants practiced by the local population. The analysis of the results allowed us to identify 78 species belonging to 36 families, the most represented are: Apiaceae (13.92%), Lamiaceae (12.65%), Asteraceae (11.39%) and Liliaceae (5.06%). The most commonly parts used from these plants are respectively: the leaves (34.21%), the stems (17.54%), the fruits (12.28%), the roots (10.52%) and the seeds (8.77%). Medicinal plants are prepared and used as powder, infusion, especially as decoction and poultice. The most used is the decoction. The main common health problem treated are; digestive disorders (29.81%), respiratory disorders (10.55%), skin inflammations (9.17%), urinary inflammations (8.25%), liver diseases (6.88%) and neurologic problems (6.42%). El Hammadia has good ethnobotanical potential of medicinal plants. This study is the first contribution to the ethnobotany of this region. We have gathered from this province some considerable knowledge about local medicinal plants for treating diseases.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122542361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The wheat quality requirements are diverse and depend on the wheat species cultivated, the expected end-use product and the environment in which it is produced. In this study, 34 bread wheat varieties were evaluated for several physico-chemical and rheological parameters including thousand-kernel weight, grain moisture content, grain protein content, wet and dry gluten contents, sedimentation Zeleny test value and Hagberg falling number. It was observed that the extreme min and max values vary depending on the genotype evaluated and the test used, suggesting the difficulty of grouping all the desirable characteristics in the same genetic background. The results also show that there was a positive correlation of the Zeleny sedimentation value with grain moisture content, protein content, wet and dry gluten at phenotypic and genotypic levels. This test can be used to predict the wheat technological quality during the milling process. Furthermore, this study offers to breeders’ valuable information which, in future breeding programs, can be used to develop high yielding and adapted genotypes, and appreciated by the rheological and technological quality of their end-use product.
{"title":"Appréciation de la qualité technologique d’une collection de blé tendre (Triticum aestivum L.) cultivée en Algérie","authors":"Asma Lamara","doi":"10.57056/ajb.v2i1.37","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.37","url":null,"abstract":"The wheat quality requirements are diverse and depend on the wheat species cultivated, the expected end-use product and the environment in which it is produced. In this study, 34 bread wheat varieties were evaluated for several physico-chemical and rheological parameters including thousand-kernel weight, grain moisture content, grain protein content, wet and dry gluten contents, sedimentation Zeleny test value and Hagberg falling number. It was observed that the extreme min and max values vary depending on the genotype evaluated and the test used, suggesting the difficulty of grouping all the desirable characteristics in the same genetic background. The results also show that there was a positive correlation of the Zeleny sedimentation value with grain moisture content, protein content, wet and dry gluten at phenotypic and genotypic levels. This test can be used to predict the wheat technological quality during the milling process. Furthermore, this study offers to breeders’ valuable information which, in future breeding programs, can be used to develop high yielding and adapted genotypes, and appreciated by the rheological and technological quality of their end-use product.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"141 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129104093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The wetland complex of Oued Righ valley accommodates a population of Ruddy Shelduck (Tadorna ferruginea) between 2012 and 2014, the total reached 80 individuals in the Lake Hamraia where the species wintering. The analysis of the diurnal time budget of wintering populations revealed the role of feeding area delivery for this species and they devoted their day time to feeding (51.2%), sleeping (18.8%), followed by swimming activity (6.3 %), and courtship activities with flying (2.03%) which often occurs after disturbances caused by human or by Marsh harrier (Circus aeruginosus). It is interesting to study the behavior of these ducks in Saharan bioclimatic area and follow the phenology and characteristics in order to preserve the biodiversity in this region.
{"title":"Ecology and behavioral response of Ruddy Shelduck (Tadorna ferruginea) in the wetland complex of Oued Righ valley (Algerian Sahara).","authors":"Y. Nouidjem","doi":"10.57056/ajb.v2i1.33","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.33","url":null,"abstract":"The wetland complex of Oued Righ valley accommodates a population of Ruddy Shelduck (Tadorna ferruginea) between 2012 and 2014, the total reached 80 individuals in the Lake Hamraia where the species wintering. The analysis of the diurnal time budget of wintering populations revealed the role of feeding area delivery for this species and they devoted their day time to feeding (51.2%), sleeping (18.8%), followed by swimming activity (6.3 %), and courtship activities with flying (2.03%) which often occurs after disturbances caused by human or by Marsh harrier (Circus aeruginosus). It is interesting to study the behavior of these ducks in Saharan bioclimatic area and follow the phenology and characteristics in order to preserve the biodiversity in this region.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126830947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Rough Bent-toed Gecko Cyrtopodion scabrum (Heyden,1827) was recently recorded in Algeria, from the east part of countries. In this note, another new locality of this species in the central Algeria (Ghardaïa region), situated at 450 km west form the last locality, based on one specimen fond in Bord of Sebkhet. C. scabrum is listed as an invasive species and it was reported as a desert species, in our finding it was recorded in an important wetland (Sebkhet El Melah) which is classified in the Ramsar list in 2004, this statement proves once again the ecological plasticity of this species.
粗弯趾壁虎Cyrtopodion scabrum (Heyden,1827)是最近在阿尔及利亚记录到的，来自东部国家。在本文中，根据在Sebkhet省采集的一个标本，该物种在阿尔及利亚中部(Ghardaïa地区)的另一个新地点位于以西450公里处，形成了最后一个地点。在2004年被列入拉姆萨尔湿地名录的重要湿地(Sebkhet El Melah)中，我们发现了该物种的记录，这再次证明了该物种的生态可塑性。
{"title":"A new locality of an invasive Gecko, Cyrtopodion scabrum (Heyden,1827) in Algeria (Squamata: Gekkonidae)","authors":"Salah Eddine Sadine","doi":"10.57056/ajb.v2i1.35","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.35","url":null,"abstract":"The Rough Bent-toed Gecko Cyrtopodion scabrum (Heyden,1827) was recently recorded in Algeria, from the east part of countries. In this note, another new locality of this species in the central Algeria (Ghardaïa region), situated at 450 km west form the last locality, based on one specimen fond in Bord of Sebkhet. C. scabrum is listed as an invasive species and it was reported as a desert species, in our finding it was recorded in an important wetland (Sebkhet El Melah) which is classified in the Ramsar list in 2004, this statement proves once again the ecological plasticity of this species.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128101310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article deals with the antimicrobial effect of Rhus tripartita (Ucria) Grande leaf extracts and total flavonoids against twelve antagonists Plant Growth Promoting Rhizobacteria of its rhizosphere, characterized in a previous study. The aim of this study is to demonstrate that leaves through their decomposition in the soil, may affect the distribution of bacterial communities in the rhizosphere. Leaves extracts were performed with distilled water, alcohol, methanol, hexane and chloroform as solvent and diluted in concentrations of 0.001, 0.01 and 0.1 mg/mL. The extraction of total flavonoids was carried out from leaves’ methanolic extract. The antimicrobial effect of the extracts was evaluated by the agar diffusion method and the determination of the minimum inhibitory concentration was carried out on a liquid medium. Alcohol, chloroform and methanol extracts were found to be the most effective on tested strains. The maximum zone inhibition was 18 mm, and the minimum zone inhibition was 7 mm. Rt 1: Bacillus licheniformis appears to be the most sensitive to all extracts. In contrast, Rt 7: Bacillus megaterium, seems to be the less sensitive strain. On the other hand, total flavonoids had a significant effect on 25% of the strains tested, mainly Bacillus genus. With a broad antimicrobial spectrum, the Rhus tripartita leaf extracts can be considered as a control agent for the distribution of the bacterial community in the rhizosphere. Therefore, our work showed that the plant could influence the bacterial diversity of its rhizosphere through its leaves.
{"title":"Influence of leaf extracts and total flavonoids of Rhus tripartita (Ucria) Grande on phytobeneficial bacteria associated with its rhizosphere","authors":"A. Benaissa","doi":"10.57056/ajb.v2i1.38","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.38","url":null,"abstract":"The article deals with the antimicrobial effect of Rhus tripartita (Ucria) Grande leaf extracts and total flavonoids against twelve antagonists Plant Growth Promoting Rhizobacteria of its rhizosphere, characterized in a previous study. The aim of this study is to demonstrate that leaves through their decomposition in the soil, may affect the distribution of bacterial communities in the rhizosphere. Leaves extracts were performed with distilled water, alcohol, methanol, hexane and chloroform as solvent and diluted in concentrations of 0.001, 0.01 and 0.1 mg/mL. The extraction of total flavonoids was carried out from leaves’ methanolic extract. The antimicrobial effect of the extracts was evaluated by the agar diffusion method and the determination of the minimum inhibitory concentration was carried out on a liquid medium. Alcohol, chloroform and methanol extracts were found to be the most effective on tested strains. The maximum zone inhibition was 18 mm, and the minimum zone inhibition was 7 mm. Rt 1: Bacillus licheniformis appears to be the most sensitive to all extracts. In contrast, Rt 7: Bacillus megaterium, seems to be the less sensitive strain. On the other hand, total flavonoids had a significant effect on 25% of the strains tested, mainly Bacillus genus. With a broad antimicrobial spectrum, the Rhus tripartita leaf extracts can be considered as a control agent for the distribution of the bacterial community in the rhizosphere. Therefore, our work showed that the plant could influence the bacterial diversity of its rhizosphere through its leaves.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134378193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biodiversity in mountains in Algeria appears scanty and has not been thoroughly investigated. However, the mountain soil has been shown as an almost entire reserve of novel enzymes with interesting properties for industrial and environmental applications. In the present study, 30 bacterial strains were isolated from the Djurdjura Mountains in Kabylia (Algeria) and were studied for their ability to produce enzymes to be possibly used in biotechnological processes such as amylase, protease, and chitinase. The characterization of these isolates was carried out using morphological, physiological, and biochemical characteristics. All the data obtained with regards to the phenotypical properties of the isolates, confirmed that the strains belonged to the Bacillus group. In addition, the 16S rRNA gene of the two retained strains KA15 and LK-DZ15 was also amplified and sequenced. Phylogenetic tree was, afterwards, constructed. The nucleotide sequences and blast analyses confirmed that the KA15 and LK-DZ15 strains were closely related to those of the Bacillus altitudinis (accession n°.: MK874318) and Paenibacillus timonensis (accession n°.: MK734103) strains. The presence of amylases, proteases, and chitinases in KA15 and LK-DZ15 isolates are an indicator of their pivotal application in a variety of biotechnological processes.
阿尔及利亚山区的生物多样性似乎很少，而且还没有得到彻底的调查。然而，山地土壤已被证明是具有工业和环境应用的有趣性质的新酶的几乎全部储备。在本研究中，从阿尔及利亚卡比利亚的Djurdjura山脉分离了30株细菌菌株，研究了它们生产酶的能力，这些酶可能用于淀粉酶、蛋白酶和几丁质酶等生物技术过程。利用形态、生理和生化特征对这些分离株进行鉴定。所有关于分离株表型特性的数据证实该菌株属于芽孢杆菌群。此外，还对保留的两株KA15和LK-DZ15的16S rRNA基因进行了扩增和测序。随后，构建了系统发育树。结果表明，KA15和LK-DZ15菌株与高海拔芽孢杆菌菌株亲缘关系密切。编号:MK874318)和timonensis Paenibacillus (accession n .;菌株:MK734103)。KA15和LK-DZ15分离株中淀粉酶、蛋白酶和几丁质酶的存在表明它们在各种生物技术过程中的关键应用。
{"title":"Characterization of endospore-forming bacteria producing extracellular enzymes isolated from Djurdjura Mountains in Algeria","authors":"Merzouk Yahiaoui","doi":"10.57056/ajb.v2i1.41","DOIUrl":"https://doi.org/10.57056/ajb.v2i1.41","url":null,"abstract":"Biodiversity in mountains in Algeria appears scanty and has not been thoroughly investigated. However, the mountain soil has been shown as an almost entire reserve of novel enzymes with interesting properties for industrial and environmental applications. In the present study, 30 bacterial strains were isolated from the Djurdjura Mountains in Kabylia (Algeria) and were studied for their ability to produce enzymes to be possibly used in biotechnological processes such as amylase, protease, and chitinase. The characterization of these isolates was carried out using morphological, physiological, and biochemical characteristics. All the data obtained with regards to the phenotypical properties of the isolates, confirmed that the strains belonged to the Bacillus group. In addition, the 16S rRNA gene of the two retained strains KA15 and LK-DZ15 was also amplified and sequenced. Phylogenetic tree was, afterwards, constructed. The nucleotide sequences and blast analyses confirmed that the KA15 and LK-DZ15 strains were closely related to those of the Bacillus altitudinis (accession n°.: MK874318) and Paenibacillus timonensis (accession n°.: MK734103) strains. The presence of amylases, proteases, and chitinases in KA15 and LK-DZ15 isolates are an indicator of their pivotal application in a variety of biotechnological processes.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131880837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this paper, 2-((3-Cyano-4,6-distyrylpyridin-2-yl)thio)acetamide (2) and its cyclized form, 3-amino-4,6-distyrylthieno[2,3-b]pyridine-2-carboxamide (3), were prepared and their structure characterizations were performed by the means of elemental and spectroscopic analyses. Their biological activity as insecticides against cowpea aphid Aphis craccivora Koch using acetamiprid insecticide as a reference was studied. The bioassay results for compounds (2) and (3) against nymphs of cowpea aphid showed that the LC50 values were 0.192 and 0.841 ppm, respectively, after 24 h of treatment but the LC50 values were 0.041 and 0.095 ppm, respectively, after 48 h of treatment. Furthermore, the bioassay results for compounds (2) and (3) showed that the LC50 values were 1.233 and 2.949 ppm, respectively, after 24 h of treatment and the LC50 values were 0.142 and 0.270 ppm, respectively, after 48 h of treatment against adults of cowpea aphid. Given these observations, it has been found that there is a remarkable relationship between the biological activity and the structure of the used compounds.
{"title":"Synthesis and biological activity of 2-((3-Cyano-4,6-distyrylpyridin-2-yl) thio) acetamide and its cyclized form","authors":"S. A. Abdel-Raheem","doi":"10.57056/ajb.v1i2.26","DOIUrl":"https://doi.org/10.57056/ajb.v1i2.26","url":null,"abstract":"In this paper, 2-((3-Cyano-4,6-distyrylpyridin-2-yl)thio)acetamide (2) and its cyclized form, 3-amino-4,6-distyrylthieno[2,3-b]pyridine-2-carboxamide (3), were prepared and their structure characterizations were performed by the means of elemental and spectroscopic analyses. Their biological activity as insecticides against cowpea aphid Aphis craccivora Koch using acetamiprid insecticide as a reference was studied. The bioassay results for compounds (2) and (3) against nymphs of cowpea aphid showed that the LC50 values were 0.192 and 0.841 ppm, respectively, after 24 h of treatment but the LC50 values were 0.041 and 0.095 ppm, respectively, after 48 h of treatment. Furthermore, the bioassay results for compounds (2) and (3) showed that the LC50 values were 1.233 and 2.949 ppm, respectively, after 24 h of treatment and the LC50 values were 0.142 and 0.270 ppm, respectively, after 48 h of treatment against adults of cowpea aphid. Given these observations, it has been found that there is a remarkable relationship between the biological activity and the structure of the used compounds.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"32 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125561449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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