This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian). The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus.
本研究旨在研究产自阿尔及利亚东南部Oued Souf地区的阿拉比卡克莱梅提取物的植物化学、抗自由基清除和抗菌活性。粗提物的甲醇提取方法有浸渍法(EM)和超声波法(EU)。收益率分别为8.95%和9.60%。总多酚定量估计值分别为11.57 mg EAG/g Ex (EM)和13.46 mg EAG/g Ex (EU)。(EM)和(EU)中总黄酮含量分别为5.48和6.19 mg EQu/g Ex. (0.1mg/ml)提取物对DPPH•自由基具有很强的清除能力，(EM)和(EU)提取物的抑制率分别为41%和60.67%。对大肠杆菌和金黄色葡萄球菌两种细菌的抑菌活性结果表明，小红花小红花对这两种细菌的抑菌效果显著，对大肠杆菌和金黄色葡萄球菌的抑菌区分别为0 ~ 12 mm和0 ~ 13 mm。
{"title":"Effects of extraction methods on total polyphenols, free radical scavenging and antibacterial activity of crude extracts of Cleome arabica L. growing in Oued Souf region","authors":"A. Chouikh","doi":"10.57056/ajb.v1i1.23","DOIUrl":"https://doi.org/10.57056/ajb.v1i1.23","url":null,"abstract":"This study aims to phytochemical study, antiradical scavenging and antibacterial Activity of the extracts of Cleome arabica L. from the region of Oued Souf (East South Algerian). The crud extracts two methods obtained methanolic were maceration (EM) and ultra-sound (EU). The yields were: 8.95% and 9.60%, respectively. The quantitative estimation of total polyphenols is 11.57 mg EAG/g Ex (EM) and 13.46 mg EAG/g Ex (EU). The flavonoids contents in (EM) and (EU) are respectively 5.48 and 6.19 mg EQu/g Ex. the anti-free radical activity in the extracts at (concentration 0.1mg/ml) showed a great capacity to scavenge of the DPPH• radical and the percentage of inhibition was 41% in (EM) and 60.67 % in (EU). The results of the antibacterial activity of two bacterial strains: Escherichia coli and Staphylococcus aureus, revealed that Cleome arabica L. has a significant effect on the two strains with inhibition zones variable from 0 to 12 mm for Escherichia coli and 0 to 13 mm for Staphylococcus aureus.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125830838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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