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RAPD analysis for genetic polymorphism in bread wheat (Triticum aestivum L. Em Thell) genotypes varying for grain protein content 小麦籽粒蛋白质含量不同基因型遗传多态性的RAPD分析
Pub Date : 2009-12-15 DOI: 10.1071/SP09009
S. Nimbal, R. K. Behl, A. Chhabra
Genetic polymorphism was investigated among nine s pring wheat genotypes, differing in grain protein c ontent, including C-306M10 ( mutant of drought tolerant va riety C306), DI 8, DI 9, DI 16, DI 20, DI 716, DI 7 17, DI 728 (near isogenic lines) and HGPC (from Wheat x R ye crosses) using 55 RAPD primers. Out of 55 primer s used, only 36 amplified and generated 2(OPG08, OP D0 5) and 12 (OPD02 ) bands. A total of 342 amplified p were observed, of which 168 were polymorphic (49.12 %) while 174 were monomorphic. The primer OPC-05 aOPC-07 revealed 92.86% and 80.00% polymorphism, respectively and t hese primers were most useful in characterization o f nine wheat genotypes included in this study. The primer OPG-08 showed no polymorphism. It is concluded that the primers OPC -05 and OPC-07 were very effective in distinguishing wheat genotypes in the present study. Twenty six RAPD primers produced a t otal of 48 unique bands for high protein content that were either present o r absent in HGPC a-high grain protein genotype and thus can be used in wheat improvement through marker-assisted select ion (for the bands which are unique by their presen ce). Data (RAPD analysis) were used to generate the similarit y coefficients using ‘siMqual’ subprogram of softwa re NTSYS-PC. The similarity coefficient values ranged from 0.97(DI8 and DI9) to 0.68 (DI9 and HGPC), indicating high genetic variability among the selected wheat genotypes. The cluster analysis and principle component analysis broadly divided the wheat genotypes into two groups and sho wed that DI 9 and HGPC were most divergent genotype s.
利用55条RAPD引物,对C306突变体c - 306m10、DI 8、DI 9、DI 16、DI 20、DI 716、DI 717、DI 728(近等基因系)和HGPC(小麦与R叶杂交)9个籽粒蛋白c含量不同的春小麦基因型的遗传多态性进行了研究。在55个引物中,只有36个扩增并产生了2个(OPG08, opd05)和12个(OPD02)条带。共扩增到342个p,其中多态性168个(49.12%),单态174个。引物OPC-05和opc -07的多态性分别为92.86%和80.00%,对9个小麦基因型的鉴定最有用。引物OPG-08无多态性。由此可见,引物OPC -05和OPC-07对小麦基因型的区分是非常有效的。26条RAPD引物共产生48条高蛋白质含量的独特条带,这些条带在HGPC(高粒蛋白基因型)中存在或不存在,因此可以通过标记辅助选择离子(用于存在的独特条带)用于小麦改良。数据(RAPD分析)采用软件re NTSYS-PC的“siMqual”子程序生成相似系数。相似系数为0.97(DI8和DI9) ~ 0.68 (DI9和HGPC),表明所选小麦基因型间具有较高的遗传变异性。聚类分析和主成分分析将小麦基因型大致分为两类,其中di9和HGPC基因型差异最大。
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引用次数: 8
Microbial degradation of polyethylene (PE) 聚乙烯(PE)的微生物降解
Pub Date : 2009-12-15 DOI: 10.1071/SP09012
K. Kumari, R. C. Aanad, N. Narula
Thirty two bacterial isolates were obtained from soil by soil burial method followed by enrichment culture technique in film culturing (FC) media. Bacterial isolates differing in morphology were selected, purified and maintained at 4°C. Thirty % of these isolates were found to be Gram negative and 50% showed positive starch hydrolysis test and were screened for their ability to degrade Low Density Polyethylene (untreated, UV and heat strips) in film culturing media and percent weight loss of polyethylene after 4th week was determined. Among various isolates, highest degradation was by Is 3, Is 22 and Is 31 to the range of 25–27%, of UV treated polyethylene strips. High temperature (40°C), was found to enhance degradation rate of polyethylene more effectively by 24–28% compared to low temperature at 30°C (18–21%). Degradation of treated polyethylene strips (UV, heat steam) was up to 4% by compost treatment as studied by using CO2 evolution, an estimation tool to analyze % degradation. Bacterial activity was also affected by various environmental factors like sunlight, temperature, oxygen etc.
采用土壤掩埋法,再采用膜培养(FC)培养基富集培养技术,从土壤中分离出32株细菌。选择不同形态的细菌分离株,纯化并在4°C下保存。其中30%的菌株革兰氏阴性,50%的菌株淀粉水解试验阳性,并对其在薄膜培养基中降解低密度聚乙烯(未经处理、紫外线和热条)的能力进行了筛选,测定了第4周后聚乙烯的失重率。在不同菌株中,is3、is22和is31对UV处理聚乙烯条的降解率最高,达到25-27%。高温(40°C)比低温(18-21%)更有效地提高了聚乙烯的降解率24-28%。通过使用CO2演化(一种分析降解率的估计工具)研究,堆肥处理对处理后的聚乙烯条(UV,热蒸汽)的降解率高达4%。光照、温度、氧气等环境因素对细菌活性也有影响。
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引用次数: 9
Effects of Nickel Salt Concentrations on Germination and Development of Grevillea exul var. rubiginosa 镍盐浓度对绿柳草发芽发育的影响
Pub Date : 2009-12-15 DOI: 10.1071/SP09003
R. Chandra, A. Tyagi, M. Bouraima-Saliou, V. Léon, R. Pineau
Endemic plant species have been considered as one of the best means of ecological restoration of exploited mine sites in New Caledonia. These plants have the adaptability characteristics that allow them to thrive on serpentine soils. This study has investigated the physiological effects of nickel concentrations at different developmental stages on Grevillea exul var. rubiginosa an endemic New Caledonian species. The first two stages of a plant life cycle germination and post germination under controlled conditions were studied. The experiment involved Petri dish germination and growth of seeds and seedlings respectively particularly root development and length in different salts of nickel (acetate, chloride and sulphate) with 0­500 ppm concentrations. Decrease in both studied parameters: germination rates and root lengths were observed with increasing concentrations of nickel solutions supplied to seeds. A minimum concentration of 5 ppm of nickel in the medium had a positive effect on germination rate as well as root length. Results suggest that it is the concentration of nickel used in the present investigation to test the germination rate and root elongation in Grevillea exul var. rubiginosa which are essential for experimental work in the field rather than the form (salt) of nickel. These results will be help-full in re-vegetation efforts of nickel mining sites in New Caledonia.
特有种植物被认为是新喀里多尼亚已开发矿区生态恢复的最佳手段之一。这些植物具有适应性特点,使它们能在蛇形土壤中茁壮成长。本文研究了不同发育阶段镍浓度对新喀里多尼亚特有种绿柳菜(Grevillea exul vars . rubiginosa)的生理影响。在控制条件下,研究了植物生命周期的前两个阶段萌发和萌发后。实验涉及培养皿中种子和幼苗的萌发和生长,特别是不同镍盐(乙酸盐、氯化物盐和硫酸盐)在0-500 ppm浓度下的根发育和长度。随着提供给种子的镍溶液浓度的增加,两个研究参数:发芽率和根长都有所下降。培养基中最低浓度为5ppm的镍对发芽率和根长都有积极的影响。结果表明,本研究采用镍的浓度来测定绿柳草的发芽率和根伸长,而不是镍的形态(盐),这是田间试验所必需的。这些结果将有助于新喀里多尼亚镍矿场地的植被恢复工作。
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引用次数: 1
Gene effects for different metric traits under normal and high temperature stress environments in wheat (T. aestivum L. Em Thell) 小麦(T. aestivum L. Em Thell)常温和高温胁迫下不同性状的基因效应
Pub Date : 2009-12-15 DOI: 10.1071/SP09007
S. Sheikh, R. K. Behl, S. S. Dhanda, Ashwani Kumar
The present investigation was conducted to analyze gene effects for grain yield and its components under two different environments. Nine wheat genotypes were selected to generate the experimental material comprised six parental and segregating generations (P1, P2, F1, F2, BC1 and BS1 of BC1 of each of the following six crosses – PBW 343 x WH 283, PBW 343 x WH 542, PBW 343 x PBW 435, UP 2565 x UP 2425, EIGN 1 x Raj 3765 and EIGN 8 x UP 2425. Joint scaling tests revealed the presence of epitasis. Six parameter model revealed the significance of additive gene effects (d) for biological and grain yield and their components in most of the crosses. The relative magnitude of (h) was higher than (d) in all the crosses for most of the characters while additive x additive (i) effects appeared to be significant for grain yield per plant (E1) and number of grain per spike (E2) in PBW 343 x WH 283. Significant (i) type of interaction was recorded in PBW 343 x WH 542 for number of grain per spike (E1), number of tillers per plant (E2) in PBW 343 x PBW 435 and for 1000-grain weight in UP 2565 x UP 2425. Pedigree method and simple selection in crosses like PBW 343 x PBW 435, UP 2565 x UP 2425, PBW 343 x WH 283 and PBW 343 x WH 542 should be used for improvement of traits governed by additive and additive x additive gene effect.
本研究分析了两种不同环境下基因对粮食产量及其构成因素的影响。选择9个小麦基因型,生成6个亲本和分离代(PBW 343 × WH 283、PBW 343 × WH 542、PBW 343 × PBW 435、UP 2565 × UP 2425、EIGN 1 × Raj 3765和EIGN 8 × UP 2425)的实验材料。关节结垢试验显示有表皮病变。六参数模型揭示了加性基因效应(d)对大多数杂交品种的生物产量和籽粒产量及其组成的显著性。多数性状的(h)的相对量级均大于(d),而加性×加性(i)效应对PBW 343 × WH 283的单株粒产量(E1)和穗粒数(E2)有显著影响。在pbw343 × WH 542中,每穗粒数(E1)、在pbw343 × pbw435中,每株分蘖数(E2)和在UP 2565 × UP 2425中,千粒重(1000粒重)均记录了显著的(i)交互作用类型。在PBW 343 × PBW 435、up2565 × up2425、PBW 343 × WH 283、PBW 343 × WH 542等组合中,应采用系谱法和简单选择的方法,对加性和加性×加性基因效应控制的性状进行改良。
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引用次数: 6
Effect of hybrids, plant density and fertility levels on nutrient concentration, uptake and productivity of pearl millet in semi arid environment. 半干旱环境下杂交种、密度和育性水平对珍珠粟养分浓度、吸收和产量的影响
Pub Date : 2009-12-15 DOI: 10.1071/SP09008
V. Rana, B. Rathore, R. K. Nanwal, Ashwani Kumar, Ramesh Vasist
A Field experiment was conducted at Crop Research F arm, Chaudhary Charan Singh Haryana Agricultural Un iversity, Hisar, during the kharif seasons of 2004 and 2005 to find out sui table spacing for pearl millet hybrids along with N and P levels so as to increase the productivity of pearl millet. The experiment was la id out in split plot design allocating combinations of two hybrids and three plant densities in main plots and four fertility levels iplots with three replications. Highest pooled grain yield was recorded by HHB 672. Sowing pearl millet at 45x12 cm spacing produced highest pooled grain and stover yield. Application of 90 kg N + 45 kg P2O5 ha -1 resulted maximum pooled grain and stover yield and was at par with 60 kg N + 30 kg P 2O5 ha -1 . HHB 67-2 had significantly higher total uptake over HHB 67. Wider spacing of 60x12 cm had higher NPK content but 45x12 cm resulted into higher total uptake of NPK. NPK content and uptake was found higher when pearl millet was fertilized with 90 kg N + 45 kg P 2O5 ha -1 .
在2004年和2005年的丰收季节,在希萨尔乔达里·查兰·辛格哈里亚纳邦农业大学作物研究中心进行了一项田间试验,以确定珍珠粟杂交种的穗间距和氮磷水平,以提高珍珠粟的产量。试验采用分割小区设计,主小区配置2个杂交种和3个株密度组合,4个育性水平小区配置3个重复。综合产量最高的品种是hb672。以45x12cm间距播种珍珠粟,籽粒和秸秆产量最高。施用90 kg N + 45 kg P2O5可获得最大的籽粒和秸秆总产量,与60 kg N + 30 kg P2O5 ha -1相当。hbb 67-2的总吸收量明显高于hbb 67。60x12 cm的氮磷钾含量较高,45x12 cm的氮磷钾总吸收量较高。以90 kg N + 45 kg p2o5 ha -1处理珍珠谷子氮磷钾含量和吸收量较高。
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引用次数: 4
Studies on biodegradation of crude oil by Aspergillus niger 黑曲霉生物降解原油的研究
Pub Date : 2009-12-15 DOI: 10.1071/SP09010
A. Mittal, Padma Singh
Hydrocarbon degrading microorganisms play a major r ole in the environment. In the present study crude oil degrading fungal strain Aspergillus niger was isolated from o il contaminated soil near crude oil production well (Lingala) Andhra Pradesh. The rate of reduction in some petroleum hy drocarbon fractions, such as n-alkanes, aromatics, nitrogen, sulfur and oxygen (NSO)-containing compounds and polycycli c aromatic hydrocarbons (PAHs), were monitored by m eans of gas chromatography. The nC 17/Pristine and n C18/Phytane ratios, extrapolated from the GC profiles decreased from the initial value of 2.510 and 7.289 to 0.132 and 0.474 respect ively at day 60.
烃类降解微生物在环境中起着重要作用。本研究从安得拉邦Lingala原油生产井附近的原油污染土壤中分离到原油降解真菌黑曲霉。用气相色谱法测定了石油烃类馏分中正构烷烃、芳烃、含氮硫氧化合物和多环芳烃的还原速率。从GC剖面推断的nC 17/Pristine和n C18/Phytane比值分别从初始值2.510和7.289下降到第60天的0.132和0.474。
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引用次数: 19
Composition and structure of the cloud forest on Mt. Delaco, Gau, Fiji 斐济高市德拉科山云雾林的组成和结构
Pub Date : 2009-12-15 DOI: 10.1071/SP09005
G. Keppel, Nunia T. Thomas
The composition and structure of cloud forest on Mt. Delaco (715m), Gau, Fiji Islands, is investigated using a transect following a ridge. Diameter (dbh), height, epiphyte cover and distance to the nearest neighbour were measured for each plant of 5 or more cm in dbh. A total of 42 vascular species were identified. The canopy was 3-5m tall, with Alpinia boia reaching 7m in height. The tree fern Dicksonia brackenridgei dominated the vegetation, accounting for 56% of the total basal area. Rapanea myricifolia, Hedycarya dorstenoides and Ascarina diffusa were other common species. Epiphytes were abundant and epiphyte cover for most trees was more than 50%. The most common epiphytes were Nephrolepis tuberosa and Nephrolepis saligna, Collospermum montanum, Peperomia spp., mosses and filmy ferns (Hymenophyllaceae) were other common species. A species of Freycinetia was the dominant climber. There was a moderately strong correlation between epiphyte richness and dbh of the host species (r = 0.59). The limited literature on cloud forest in the tropical insular Pacific suggests that while many taxa are shared, the species composition may vary considerably on different mountains.
采用沿山脊的样带对斐济群岛高(Gau)德拉科山(715米)云雾林的组成和结构进行了调查。测量了直径(dbh)、高度、附生盖度和到最近邻居的距离。共鉴定出42种维管植物。冠层高3-5米,其中高山植物高达7米。植被以蕨类为主,占总面积的56%。杨梅Rapanea myricifolia、桃金娘(Hedycarya dorstenoides)和白花天冬(Ascarina diffusa)是常见种。附生植物丰富,大多数乔木的附生覆盖度在50%以上。最常见的附生植物为结节肾松和盐渍肾松,其他常见种为胶菇、胡椒属,苔藓和膜蕨(膜蕨科)。在攀援植物中占优势的是一种Freycinetia。附生真菌丰富度与寄主物种的胸径有较强的相关性(r = 0.59)。关于热带岛屿太平洋云雾林的有限文献表明,虽然许多分类群是共享的,但不同山脉的物种组成可能存在很大差异。
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引用次数: 1
Maximization of poly-β-hydroxy butyrate production by rhizo-bacteria using eco-friendly agri-byproducts 利用生态友好型农业副产品的根瘤菌最大化生产聚β-羟基丁酸酯
Pub Date : 2009-12-15 DOI: 10.1071/SP09006
U. Kumar, Aarti Sharma, V. Sikka
Attempts have been made, using rhizobacteria, to de velop a protocol for economical polyhydroxybutyrate production using agribyproducts. The optimum conditions for maximum PHB production were found with modified glucose minimal media. A cost effective media using exclusively readily available agri-bypr oducts yielding as much as 60 mg/l PHB (which corre spond to 53.1 % of the biomass) from isolates of rhizobacteria has been de veloped
利用根瘤菌,开发了一种利用农副产物生产聚羟基丁酸酯的经济方案。以改性葡萄糖为培养基,找到了PHB产量最高的最佳条件。已经开发出一种具有成本效益的培养基,该培养基仅使用易于获得的农业产品,从根杆菌分离株中产生高达60 mg/l的PHB(相当于生物量的53.1%)
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引用次数: 2
The annual effective dose due to natural radionuclides in the reservoir and tap water in Yaoundé area, Cameroon 喀麦隆雅温达尔地区水库和自来水中天然放射性核素的年有效剂量
Pub Date : 2009-12-15 DOI: 10.1071/SP09011
R. Lydie, R. M. Nemba
The average concentrations of radionuclides of reservoir and tap water in the Yaounde area, with a population of 1.5 million inhabitants were estimated from measurements of mean specific activity using a well calibrated Canberra NaI(Tl) detector system. Water samples were collected from reservoirs and taps during the dry and the rainy seasons respectively in December 2002 and July 2003. The radionuclides observed with regularity belonged to the decay series naturally occurring radionuclides headed by 238U and 232Th as well as the non-series nuclide 40K. The average specific activity values obtained for 40K, 226Ra and 228Ra, respectively were for reservoir water 70 ± 11 BqL–1; 8.7 ± 3.5 BqL–1; 0.6 ± 0.2 BqL–1 during the dry season, and 50 ± 9 BqL–1; 8.5 ± 3.7 BqL–1; 0.6 ± 0.2 BqL–1 during the rainy season and for tap water, we have respectively 111 ± 17 BqL–1; 11.4 ± 3.7 BqL–1; 1 ± 0.3 BqL–1 during the dry season, and 51 ± 10 BqL–1; 9 ± 3.5 BqL–1; 0.7 ± 0.2 BqL–1 during the rainy season. The annual effective dose received by Yaounde adult population as a result of ingestion of this drinking water is respectively 0.925 mSv for the reservoir water and 1.052 mSv for the tap water.
使用校准良好的堪培拉NaI(Tl)探测器系统对平均比活度进行测量,估计了雅温得地区水库和自来水中放射性核素的平均浓度,该地区有150万居民。本署分别于2002年12月及2003年7月在旱季及雨季从水塘及水龙头采集水样。观察到的有规律的放射性核素属于以238U和232Th为首的自然衰变系列放射性核素以及40K非系列核素。40K、226Ra和228Ra对水库水的平均比活度分别为70±11 BqL-1;8.7±3.5 BqL-1;旱季为0.6±0.2 BqL-1,旱季为50±9 BqL-1;8.5±3.7 BqL-1;雨季为0.6±0.2 BqL-1,自来水为111±17 BqL-1;11.4±3.7 BqL-1;旱季为1±0.3 BqL-1,旱季为51±10 BqL-1;9±3.5 BqL-1;雨季0.7±0.2 BqL-1。雅温得成年人口因饮用该饮用水而受到的年有效剂量分别为水库水0.925毫西弗和自来水1.052毫西弗。
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引用次数: 6
Trace element concentrations in sediments from Kadavu passage, Fiji 斐济Kadavu通道沉积物中微量元素的浓度
Pub Date : 2009-12-15 DOI: 10.1071/SP09001
S. Garimella, R. Lal, R. Deo, R. Morrison
Grab sediments, collected from 11 locations from the Pacific Ocean floor in the region between Suva Peninsula and Kadavu Island, Fiji, were investigated for concentrations of 20 elements using neutron activation and high-resolution gamma ray spectroscopy techniques. The sediments were collected at distances between 5-35 km from the mainland and at ocean depths ranging from 500 to 2000 m. A comparison of the elemental abundances of these sediments with those of the Suva lagoon and other nearby locations indicates that the ocean sediments are likely to be derived from sediments washed out to sea from the mainland. The sediments also show no evidence of any metal contamination from the industrial activity in the Suva metropolitan area.
研究人员利用中子活化和高分辨率伽马射线能谱技术,从斐济苏瓦半岛和卡达武岛之间的太平洋海底11个地点收集了Grab沉积物,研究了20种元素的浓度。这些沉积物是在距离大陆5-35公里和海洋深度500 - 2000米的地方收集的。将这些沉积物的元素丰度与苏瓦泻湖和其他附近地区的元素丰度进行比较表明,海洋沉积物很可能来自从大陆冲到海洋的沉积物。沉积物也没有显示苏瓦市区工业活动造成任何金属污染的证据。
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引用次数: 2
期刊
The South Pacific Journal of Natural Science
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