Pub Date : 2019-11-06DOI: 10.24843/atbes.2018.v02.i02.p05
N. Watiniasih, M. Rohmah
Sun Bear (Helarctos malayanus) is found in Indonesia such as in Sumatra and Kalimantan. Helarctos malayanus is the smallest bear in the world and listed as vulnerable animal. Baloo is a sun bear that is rescued and raised at Bali Animal Rescue Centre located in Tabanan Bali. Before it will be released to the natural habitat, it is important to understand its behaviour, so it will be survived in their natural habitat. This study aimed to investigate the daily activity of sun bear rais at Bali Animal Rescue Centre. Research has been conducted at Bali Animal Rescue Centre from 2nd – 26th January 2019. Observation o daily activities of sun bear was conducted from 08.00 – 16.00 local time. It has been found that most of daily time was spent on grooming (35%), followed by feeding/eating (27%), moving (26%), and stationary (24%). Baloo has hardly found vocalized or defacate/urinate. Baloo was resting around 11.00 am and active feeding/eating along the day as well as moving. She moved a lot at 08.00 am, as she was provisioned by the carer, less moving in the middle of the day (12.00 am) and before resting at 17.00 pm. The variation of time spent on the daily activities of the sun bear at the rescue center can be used as reference before she will be release to its natural habitat.
{"title":"The Daily Activities of Sun Bear (Helarctos malayanus, Ursidae) in Bali Animal Rescue Center","authors":"N. Watiniasih, M. Rohmah","doi":"10.24843/atbes.2018.v02.i02.p05","DOIUrl":"https://doi.org/10.24843/atbes.2018.v02.i02.p05","url":null,"abstract":"Sun Bear (Helarctos malayanus) is found in Indonesia such as in Sumatra and Kalimantan. Helarctos malayanus is the smallest bear in the world and listed as vulnerable animal. Baloo is a sun bear that is rescued and raised at Bali Animal Rescue Centre located in Tabanan Bali. Before it will be released to the natural habitat, it is important to understand its behaviour, so it will be survived in their natural habitat. This study aimed to investigate the daily activity of sun bear rais at Bali Animal Rescue Centre. Research has been conducted at Bali Animal Rescue Centre from 2nd – 26th January 2019. Observation o daily activities of sun bear was conducted from 08.00 – 16.00 local time. It has been found that most of daily time was spent on grooming (35%), followed by feeding/eating (27%), moving (26%), and stationary (24%). Baloo has hardly found vocalized or defacate/urinate. Baloo was resting around 11.00 am and active feeding/eating along the day as well as moving. She moved a lot at 08.00 am, as she was provisioned by the carer, less moving in the middle of the day (12.00 am) and before resting at 17.00 pm. The variation of time spent on the daily activities of the sun bear at the rescue center can be used as reference before she will be release to its natural habitat.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133688633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-27DOI: 10.24843/atbes.2019.v03.i02.p05
K. Agustina, Ida Ayu Putu Aselya Mardyawati, I. K. Suada
This research aims to produce salted eggs with antioxidant content from bay leave extract. The study used a completely randomized design (CRD) in which there were 3 treatments, namely, control with 0% bay leaf extract, treatment 1 with adding 25% crude extract of bay leaves, and treatment 2 with a 50% addition of crude bay leaf extract. All treatments were pressed for 14 days before testing. The parameters observed in the study were the antioxidant capacity, albumin index, yolk index, and Haugh unit. The results show that the sated eggs with the different concentration of bay leave extract contain antioxidant 4.445%, 30.853%, and 44.32% respectively, albumin index of treated eggs was lower than control, while Haugh unit of treated eggs was higher than control, and no differentiation of yolk index between the treatments. To conclude, the addition of bay leave crude extract can increase the value of salted eggs without disturbing the quality.
{"title":"The Quality and Antioxidant Content of Salted Eggs Made By Addition of Bay Leave Crude Extract on the Salting Media","authors":"K. Agustina, Ida Ayu Putu Aselya Mardyawati, I. K. Suada","doi":"10.24843/atbes.2019.v03.i02.p05","DOIUrl":"https://doi.org/10.24843/atbes.2019.v03.i02.p05","url":null,"abstract":"This research aims to produce salted eggs with antioxidant content from bay leave extract. The study used a completely randomized design (CRD) in which there were 3 treatments, namely, control with 0% bay leaf extract, treatment 1 with adding 25% crude extract of bay leaves, and treatment 2 with a 50% addition of crude bay leaf extract. All treatments were pressed for 14 days before testing. The parameters observed in the study were the antioxidant capacity, albumin index, yolk index, and Haugh unit. The results show that the sated eggs with the different concentration of bay leave extract contain antioxidant 4.445%, 30.853%, and 44.32% respectively, albumin index of treated eggs was lower than control, while Haugh unit of treated eggs was higher than control, and no differentiation of yolk index between the treatments. To conclude, the addition of bay leave crude extract can increase the value of salted eggs without disturbing the quality.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114823329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-24DOI: 10.24843/atbes.2019.v03.i02.p04
Ni Made Sastriyani Wiendra, M. Pharmawati
Impatiens balsamina L. is a plant that is widely cultivated in Bali. The flower of this plant is used as a component of offering in Hindu religious ceremonies. The flower petals are thin and wilt easily, therefore genetic modification is needed to get better quality. This research aimed to study the effect of 0.01% colchicine with soaking periods of 4 hours, 8 hours, 12 hours and 24 hours to seedlings of I. balsamina. Seeds of I. balsamina were germinated and sprouted seeds were treated with 0.01% colchicine for 4 hours, 8 hours, 12 hours and 24 hours. The sprouted seeds were then transfer to polybag with soil media. The experiment was designed with randomized blok design with five replicates. Observations were done on plant height, length and width of leaf, stem diameter, number of stomata as well as observation on plant stem cortex cells. Results showed that colchicine reduced plant height at three weeks after planting, while plant stem diameter increased. Control plants had the smallest leaf length and width. The number of stomata decreased at plants derived from colchicine treated sprouted seeds and the lowest stomatal number observed was at soaking duration of 24 hours.
{"title":"Morphological and Anatomical Changes by Cochicine in Seedling of Impatiens balsamina L.","authors":"Ni Made Sastriyani Wiendra, M. Pharmawati","doi":"10.24843/atbes.2019.v03.i02.p04","DOIUrl":"https://doi.org/10.24843/atbes.2019.v03.i02.p04","url":null,"abstract":"Impatiens balsamina L. is a plant that is widely cultivated in Bali. The flower of this plant is used as a component of offering in Hindu religious ceremonies. The flower petals are thin and wilt easily, therefore genetic modification is needed to get better quality. This research aimed to study the effect of 0.01% colchicine with soaking periods of 4 hours, 8 hours, 12 hours and 24 hours to seedlings of I. balsamina. Seeds of I. balsamina were germinated and sprouted seeds were treated with 0.01% colchicine for 4 hours, 8 hours, 12 hours and 24 hours. The sprouted seeds were then transfer to polybag with soil media. The experiment was designed with randomized blok design with five replicates. Observations were done on plant height, length and width of leaf, stem diameter, number of stomata as well as observation on plant stem cortex cells. Results showed that colchicine reduced plant height at three weeks after planting, while plant stem diameter increased. Control plants had the smallest leaf length and width. The number of stomata decreased at plants derived from colchicine treated sprouted seeds and the lowest stomatal number observed was at soaking duration of 24 hours.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128230807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.24843/atbes.2019.v03.i02.p02
Kadek Ayu Trisna Yanti, I. Setyawati, Ni Putu Adriani Astiti
This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.
{"title":"Lung Histopathology of Laying Hens Infected by Colibacillosis in the Animal Cages Experiments of the Disease Investigation Center 6, Denpasar, Bali","authors":"Kadek Ayu Trisna Yanti, I. Setyawati, Ni Putu Adriani Astiti","doi":"10.24843/atbes.2019.v03.i02.p02","DOIUrl":"https://doi.org/10.24843/atbes.2019.v03.i02.p02","url":null,"abstract":"This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131468044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-20DOI: 10.24843/atbes.2019.v03.i02.p03
I. W. Santiyasa, Luh Eka Kusumayanti
The concept of a smart city is indeed presented as an answer for efficient management of resources. Support for applications that are constantly evolving and the creation of a creative ecosystem in the field of technology, is a good first step towards a smart city. But in reality smart city is not only related to technology. This concept is a combination of new technology and intelligent thinking about the use of technology. As a city full of allure, Denpasar City along with its development and population growth, began to emerge various problems such as decreasing the quality of public services, congestion on the road, accumulation of garbage and other social problems. To solve these problems, Denpasar needs a smart, creative and innovative solution run by the ranks of government officials, from leaders to the lowest levels, and supported by the full commitment of all its citizens. Various efforts have been made by the Denpasar City government to facilitate services to the community, ranging from building a system to facilitate services to the community such as the health service system, population service system, government service system (e-Gove), and the public complaints system. To find out whether the efforts made by the government are related to the services provided to the community by implementing a smart city system. In this study various measurements of satisfaction levels were carried out to obtain significant conclusions. Of the five aspects studied, namely aspects of government services, aspects of government transparency, aspects of health services, aspects of population service, aspects of transportation services and aspects of water supply and electricity services, in general the people of Denpasar expressed satisfaction with a level of satisfaction of 76,312 and the level of satisfaction with aspects of health services have the highest level of satisfaction that is equal to 88,574.
{"title":"Implementation of ANN-CFB Methods in Measuring Community Satisfaction Level of Denpasar City on the Aspect of Government Services","authors":"I. W. Santiyasa, Luh Eka Kusumayanti","doi":"10.24843/atbes.2019.v03.i02.p03","DOIUrl":"https://doi.org/10.24843/atbes.2019.v03.i02.p03","url":null,"abstract":"The concept of a smart city is indeed presented as an answer for efficient management of resources. Support for applications that are constantly evolving and the creation of a creative ecosystem in the field of technology, is a good first step towards a smart city. But in reality smart city is not only related to technology. This concept is a combination of new technology and intelligent thinking about the use of technology. As a city full of allure, Denpasar City along with its development and population growth, began to emerge various problems such as decreasing the quality of public services, congestion on the road, accumulation of garbage and other social problems. To solve these problems, Denpasar needs a smart, creative and innovative solution run by the ranks of government officials, from leaders to the lowest levels, and supported by the full commitment of all its citizens. Various efforts have been made by the Denpasar City government to facilitate services to the community, ranging from building a system to facilitate services to the community such as the health service system, population service system, government service system (e-Gove), and the public complaints system. To find out whether the efforts made by the government are related to the services provided to the community by implementing a smart city system. In this study various measurements of satisfaction levels were carried out to obtain significant conclusions. Of the five aspects studied, namely aspects of government services, aspects of government transparency, aspects of health services, aspects of population service, aspects of transportation services and aspects of water supply and electricity services, in general the people of Denpasar expressed satisfaction with a level of satisfaction of 76,312 and the level of satisfaction with aspects of health services have the highest level of satisfaction that is equal to 88,574.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125440336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-04DOI: 10.24843/atbes.2019.v03.i02.p01
I. W. Sudira, I. M. Merdana, Suci Nur Qurani
Guava plants (Psidium guajava L) are fruit plants originating from South America and can thrive in the territory of Indonesia. Now the biggest center for guava cultivation is spread in DKI Jakarta, West Java, Central Java, East Java, Yogyakarta, Bali, West Nusa Tenggara, Sumatra and Kalimantan. In addition to taking the fruit, guava leaves are also used as a traditional medicine for the treatment of diarrhea in human and animals. This study aims to identify active compounds in guava leaves. That is a secondary metabolites with pharmacological effects as anti diarrhea such as flavonoid, alkaloid, tannins and essential oils. The leaves sample used in this study were taken from farmers in the West Denpasar area, Bali. Extraction of guava leaf leaves using 96% ethanol, and phytochemical analysis was carried out to detect the presence of active compounds. The data obtained is presented and analyzed descriptively qualitatively. The results showed that the ethanol extract of guava leaves contained active compound inclaude steroid/triterpenoid, phenolics, alkaloids, flavonoids, saponins and tannins. It can be concluded that guava leaf extract contains are potential antidiarrheal
{"title":"Preliminary Phitochemical Analysis Of Guava Leaves (Psidium guajava L.) Extract As Antidiarrheal In Calves","authors":"I. W. Sudira, I. M. Merdana, Suci Nur Qurani","doi":"10.24843/atbes.2019.v03.i02.p01","DOIUrl":"https://doi.org/10.24843/atbes.2019.v03.i02.p01","url":null,"abstract":"Guava plants (Psidium guajava L) are fruit plants originating from South America and can thrive in the territory of Indonesia. Now the biggest center for guava cultivation is spread in DKI Jakarta, West Java, Central Java, East Java, Yogyakarta, Bali, West Nusa Tenggara, Sumatra and Kalimantan. In addition to taking the fruit, guava leaves are also used as a traditional medicine for the treatment of diarrhea in human and animals. This study aims to identify active compounds in guava leaves. That is a secondary metabolites with pharmacological effects as anti diarrhea such as flavonoid, alkaloid, tannins and essential oils. The leaves sample used in this study were taken from farmers in the West Denpasar area, Bali. Extraction of guava leaf leaves using 96% ethanol, and phytochemical analysis was carried out to detect the presence of active compounds. The data obtained is presented and analyzed descriptively qualitatively. The results showed that the ethanol extract of guava leaves contained active compound inclaude steroid/triterpenoid, phenolics, alkaloids, flavonoids, saponins and tannins. It can be concluded that guava leaf extract contains are potential antidiarrheal","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115881944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.24843/ATBES.2019.V03.I01.P05
N. Wijayanti, I. Putra
Abstract. Heavy metal contamination is a major threat for marine ecosystem that directly affecting the organisms’ life. Some of marine organisms have an ability to response the heavy metal contamination in water, and could be used as bio-indicator. One of them is seagrass that used in this study to evaluate the water quality in Sanur beach. This study was held in Sanur beach, Denpasar, from July-August 2018. Sampling location determined by purposive sampling method and generated 4 research stations. Sediments and seagrass leaves (Enhalus acoroides) was collected and analyzed in Analytical Laboratory Udayana University using ICPE-9000. Water quality parameters measured in situ (temperature and salinity) and in Medical Laboratory Bali Province (pH and DO). The results showed if the water quality in all research stations were in safety range based on Governor Regulation No. 16 of 2016 about marine water quality for tourism and recreation. The highest content of heavy metal (Pb) in sediment is 16.207 mg kg-1 that possessed by the 1st station, while the lowest is 14.318 mg kg-1 at the 3rd station. The highest Pb content in seagrass leaves at the 2nd station is 5.646 mg kg-1, and the lowest at the 1st station is 4.926 mg kg-1. The 3rd station sediment had the highest Cd content is 2.252 mg kg-1 and the 4th station had the lowest is 2.044 mg kg-1, while for leaves with the highest Cd content was at the 4th station is 0.552 mg kg-1 and the lowest at the 3rd station is 0.458 mg kg-1. The Cu content in sediment was highest at the 1st station is 11.533 mg kg-1 and the lowest at the 3rd station is 8.501 mg kg-1. For leaves’ Cu content, the highest amount of Cu is 3.699 mg kg-1 at the 4th station, while the lowest at the 2nd station with 2.570 mg kg-1.
{"title":"Seagrass (Enhalus acoroides) as an Heavy Metal Bioindicator on Biomonitoring Water Quality in Sanur Beach Bali","authors":"N. Wijayanti, I. Putra","doi":"10.24843/ATBES.2019.V03.I01.P05","DOIUrl":"https://doi.org/10.24843/ATBES.2019.V03.I01.P05","url":null,"abstract":"Abstract. Heavy metal contamination is a major threat for marine ecosystem that directly affecting the organisms’ life. Some of marine organisms have an ability to response the heavy metal contamination in water, and could be used as bio-indicator. One of them is seagrass that used in this study to evaluate the water quality in Sanur beach. This study was held in Sanur beach, Denpasar, from July-August 2018. Sampling location determined by purposive sampling method and generated 4 research stations. Sediments and seagrass leaves (Enhalus acoroides) was collected and analyzed in Analytical Laboratory Udayana University using ICPE-9000. Water quality parameters measured in situ (temperature and salinity) and in Medical Laboratory Bali Province (pH and DO). The results showed if the water quality in all research stations were in safety range based on Governor Regulation No. 16 of 2016 about marine water quality for tourism and recreation. The highest content of heavy metal (Pb) in sediment is 16.207 mg kg-1 that possessed by the 1st station, while the lowest is 14.318 mg kg-1 at the 3rd station. The highest Pb content in seagrass leaves at the 2nd station is 5.646 mg kg-1, and the lowest at the 1st station is 4.926 mg kg-1. The 3rd station sediment had the highest Cd content is 2.252 mg kg-1 and the 4th station had the lowest is 2.044 mg kg-1, while for leaves with the highest Cd content was at the 4th station is 0.552 mg kg-1 and the lowest at the 3rd station is 0.458 mg kg-1. The Cu content in sediment was highest at the 1st station is 11.533 mg kg-1 and the lowest at the 3rd station is 8.501 mg kg-1. For leaves’ Cu content, the highest amount of Cu is 3.699 mg kg-1 at the 4th station, while the lowest at the 2nd station with 2.570 mg kg-1.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124986803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-26DOI: 10.24843/ATBES.2019.V03.I01.P04
I. M. O. Riawan, G. I. Setiabudi, I. M. Merdana, I. P. M. Mariasa, Kadek Teguh Wirasastra
Stranded Sunfish in North Bali with full body we collect to do molecular identification. Samples were amplified at the d-loop locus (control region) using the PCR (Polymerase Chain Reaction) method. Primers used in PCR are H16498 as primary front (forward) and L15812 as reverse primer. Similarity value of 95% after alignment with Mola ramsayi (accession number accession AY940824) on GenBank, and the gaps of the nucleotide just 1%. The stranded sunfish identified using partial sequence mtDNA is the same species as the species Mola ramsayi.
{"title":"First Molecular Identification of Sunfish in North Bali Water","authors":"I. M. O. Riawan, G. I. Setiabudi, I. M. Merdana, I. P. M. Mariasa, Kadek Teguh Wirasastra","doi":"10.24843/ATBES.2019.V03.I01.P04","DOIUrl":"https://doi.org/10.24843/ATBES.2019.V03.I01.P04","url":null,"abstract":"Stranded Sunfish in North Bali with full body we collect to do molecular identification. Samples were amplified at the d-loop locus (control region) using the PCR (Polymerase Chain Reaction) method. Primers used in PCR are H16498 as primary front (forward) and L15812 as reverse primer. Similarity value of 95% after alignment with Mola ramsayi (accession number accession AY940824) on GenBank, and the gaps of the nucleotide just 1%. The stranded sunfish identified using partial sequence mtDNA is the same species as the species Mola ramsayi.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124358722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-26DOI: 10.24843/ATBES.2019.V03.I01.P03
Vanesa Vanesa Martida, M. Pharmawati
DNA extraction that gives good quantity and quality DNA is a basic step that must be completed for molecular studies, especially in DNA fingerprint imaging. The aim of this research was to find out the better quality and quantity of DNA extracted from different plant materials of frangipani cultivars (Plumeria sp.). Leaves and flowers were collected from Taman Jepun, Denpasar Bali. Fresh young leaves and flowers were used as plant materials as well as dried leaves (silica gel dried leaves) of Plumeria sp. This research used CTAB buffer with modification as lysis buffer. Purification techique used NucleoSpin® Gel and PCR Clean Up Kit. The results showed that the colour of DNA solution from fresh material was clear and the quantities of DNA from young fresh leaves were between 70-300 ng/µl. The DNA colour solution from flowers was also transparent with concentration between 0-40 ng/µl. DNA isolated from dry material resulted in brown solution with DNA quantity between 30-100 ng/µl and need to be purified to obtain clear DNA solution.
DNA提取是分子研究，特别是DNA指纹成像必须完成的基本步骤。本研究的目的是探讨从鸡蛋花(Plumeria sp.)不同植物材料中提取DNA的最佳质量和数量。叶和花采自巴厘岛登巴萨的塔曼杰潘。以鸡毛花的鲜嫩叶片和花为原料，以干叶(硅胶干叶)为原料，采用改性CTAB缓冲液作为裂解缓冲液。纯化技术采用NucleoSpin®凝胶和PCR Clean Up Kit。结果表明，新鲜材料DNA溶液颜色清晰，鲜叶DNA含量在70 ~ 300 ng/µl之间。从花中提取的DNA显色液也是透明的，浓度在0-40 ng/µl之间。从干燥材料中分离的DNA得到棕色溶液，DNA含量在30-100 ng/µl之间，需要纯化以获得清晰的DNA溶液。
{"title":"Comparison of DNA Yield from Different Plant Materials of Plumeria sp. (Apocynaceae)","authors":"Vanesa Vanesa Martida, M. Pharmawati","doi":"10.24843/ATBES.2019.V03.I01.P03","DOIUrl":"https://doi.org/10.24843/ATBES.2019.V03.I01.P03","url":null,"abstract":"DNA extraction that gives good quantity and quality DNA is a basic step that must be completed for molecular studies, especially in DNA fingerprint imaging. The aim of this research was to find out the better quality and quantity of DNA extracted from different plant materials of frangipani cultivars (Plumeria sp.). Leaves and flowers were collected from Taman Jepun, Denpasar Bali. Fresh young leaves and flowers were used as plant materials as well as dried leaves (silica gel dried leaves) of Plumeria sp. This research used CTAB buffer with modification as lysis buffer. Purification techique used NucleoSpin® Gel and PCR Clean Up Kit. The results showed that the colour of DNA solution from fresh material was clear and the quantities of DNA from young fresh leaves were between 70-300 ng/µl. The DNA colour solution from flowers was also transparent with concentration between 0-40 ng/µl. DNA isolated from dry material resulted in brown solution with DNA quantity between 30-100 ng/µl and need to be purified to obtain clear DNA solution.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125958291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-21DOI: 10.24843/ATBES.2019.V03.I01.P01
I. Setyawati
For cattle, red calliandra (Calliandra calothyrsus) is a good protein source of forage but the condensed tannin content is the highest compared to other legume plants. Tannin can reduce the activity of digestive enzymes and protein digestibility, which can lead to pancreatic hypertrophy and hyperplasia. This study aimed to determine the pancreatic histology of pregnant rats treated with C.calothyrsus leaf flour during pregnancy and lactation period. This study used a completely randomized design with 24 pregnant female rats which were divided into four groups i.e. control and treatments of 10, 17.5 and 25% of C.calothyrsus leaf flour in the diets. The treatment started from the first day of pregnancy until the young rats were weaned at the age of 21 days. Pancreatic histological structure of the mother showed an increase lack of normal tissue density and an increase connective tissues in the pancreas descriptively, especially at the highest level of C.calothyrsus leaf flour (25%) in the diets.
{"title":"Histology of Rat Pancreas Treated with Calliandra calothyrsus Leaf in the Diets during Pregnancy and Lactation","authors":"I. Setyawati","doi":"10.24843/ATBES.2019.V03.I01.P01","DOIUrl":"https://doi.org/10.24843/ATBES.2019.V03.I01.P01","url":null,"abstract":"For cattle, red calliandra (Calliandra calothyrsus) is a good protein source of forage but the condensed tannin content is the highest compared to other legume plants. Tannin can reduce the activity of digestive enzymes and protein digestibility, which can lead to pancreatic hypertrophy and hyperplasia. This study aimed to determine the pancreatic histology of pregnant rats treated with C.calothyrsus leaf flour during pregnancy and lactation period. This study used a completely randomized design with 24 pregnant female rats which were divided into four groups i.e. control and treatments of 10, 17.5 and 25% of C.calothyrsus leaf flour in the diets. The treatment started from the first day of pregnancy until the young rats were weaned at the age of 21 days. Pancreatic histological structure of the mother showed an increase lack of normal tissue density and an increase connective tissues in the pancreas descriptively, especially at the highest level of C.calothyrsus leaf flour (25%) in the diets.","PeriodicalId":432816,"journal":{"name":"Advances in Tropical Biodiversity and Environmental Sciences","volume":"137 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116550334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：