Pub Date : 2018-12-15DOI: 10.15835/BUASVMCN-FST:2018.0006
C. Beinșan, R. Sumalan, S. Vâtcă
Garlic is an asexually propagated crop with high morphological diversity and uses throughout the world. Although the salt tolerance among the cultivated species is low, there are some genotypes which present an acceptable level of adaptability to moderate saline conditions. In vegetables, these genotypes are represented especially by local varieties breeded by local farmers. It is therefore necessary to achieve a balance between productivity and salinity tolerance, for the obtaining of new genotypes with high productivity. Our experiments aimed to study the main physiological and biochemical indicators for the identification of salinity tolerant genotypes in a collection of local autumn and spring garlic populations from Timis County. The local populations studied show a wide genetic diversity based on synthesis of free proline, by selecting genotypes with good salinity tolerance for five autumn garlic varieties and five for spring garlic. There are major differences both morphologically and physiologically, between studied varieties of halophytes. Identification of these cultivars may represent a resource for breeding programs of cultivated vegetables on saline soils.
{"title":"The Influence of Osmotic Stress on Physiological and Biochemical Indices at Garlic (Allium sativum L.) Local Populations","authors":"C. Beinșan, R. Sumalan, S. Vâtcă","doi":"10.15835/BUASVMCN-FST:2018.0006","DOIUrl":"https://doi.org/10.15835/BUASVMCN-FST:2018.0006","url":null,"abstract":"Garlic is an asexually propagated crop with high morphological diversity and uses throughout the world. Although the salt tolerance among the cultivated species is low, there are some genotypes which present an acceptable level of adaptability to moderate saline conditions. In vegetables, these genotypes are represented especially by local varieties breeded by local farmers. It is therefore necessary to achieve a balance between productivity and salinity tolerance, for the obtaining of new genotypes with high productivity. Our experiments aimed to study the main physiological and biochemical indicators for the identification of salinity tolerant genotypes in a collection of local autumn and spring garlic populations from Timis County. The local populations studied show a wide genetic diversity based on synthesis of free proline, by selecting genotypes with good salinity tolerance for five autumn garlic varieties and five for spring garlic. There are major differences both morphologically and physiologically, between studied varieties of halophytes. Identification of these cultivars may represent a resource for breeding programs of cultivated vegetables on saline soils.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90452212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.15835/BUASVMCN-FST:2018.0019
A. Cecan, A. Pârvu, M. Pârvu, F. E. Fischer, M. Pațiu, F. Cătoi, A. Irimie
Natural products were proved to have inhibitory effect on the nitro-oxidative stress. The aim of the study was to evaluate the effect of Mahonia aquifolium (MA) flowers extract upon nitro-oxidative stress in acute experimental inflammation. The extract was prepared by repercolation method. Acute experimental inflammation was induced with turpentine oil (0,6ml/kg b.w. i.m.). MA extract was given for 7 days. Were used 6 groups (n=5) of male Wistar rats: Groups 1-3 were with acute inflammation and treated with MA dilutions (100%, 50%, 25%); Group 4 was acute inflammation control; Group 5 was negative control; Group 6 was acute inflammation treated with diclofenac (10mg/kg b.w. p.o). In day 8 nitro-oxidative stress was evaluated by measuring serum nitrites and nitrates (NOx), Total oxidative stress (TOS), Total antioxidant capacity (TAC), Oxidative stress index (OSI), Malondialdehyde (MDA) and Thiols (SH). MA reduced OSI and TOS, increased SH, and had no important effect on TAC, NO and MDA. Compared to MA, Diclofenac was a stronger inhibitor of TOS and OSI, and had a smaller effect on SH. Mahonia aquifolium flowers extract had inhibitory effect on the oxidative stress, without influencing NO and lypoperoxides production, the effect being smaller than that of Diclofenac.
{"title":"Mahonia Aquifolium Flowers Extract Effects in Acute Experimental Inflammation","authors":"A. Cecan, A. Pârvu, M. Pârvu, F. E. Fischer, M. Pațiu, F. Cătoi, A. Irimie","doi":"10.15835/BUASVMCN-FST:2018.0019","DOIUrl":"https://doi.org/10.15835/BUASVMCN-FST:2018.0019","url":null,"abstract":"Natural products were proved to have inhibitory effect on the nitro-oxidative stress. The aim of the study was to evaluate the effect of Mahonia aquifolium (MA) flowers extract upon nitro-oxidative stress in acute experimental inflammation. The extract was prepared by repercolation method. Acute experimental inflammation was induced with turpentine oil (0,6ml/kg b.w. i.m.). MA extract was given for 7 days. Were used 6 groups (n=5) of male Wistar rats: Groups 1-3 were with acute inflammation and treated with MA dilutions (100%, 50%, 25%); Group 4 was acute inflammation control; Group 5 was negative control; Group 6 was acute inflammation treated with diclofenac (10mg/kg b.w. p.o). In day 8 nitro-oxidative stress was evaluated by measuring serum nitrites and nitrates (NOx), Total oxidative stress (TOS), Total antioxidant capacity (TAC), Oxidative stress index (OSI), Malondialdehyde (MDA) and Thiols (SH). MA reduced OSI and TOS, increased SH, and had no important effect on TAC, NO and MDA. Compared to MA, Diclofenac was a stronger inhibitor of TOS and OSI, and had a smaller effect on SH. Mahonia aquifolium flowers extract had inhibitory effect on the oxidative stress, without influencing NO and lypoperoxides production, the effect being smaller than that of Diclofenac.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78897274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.15835/BUASVMCN-FST:2018.0012
S. Andrei, A. Bunea, C. Bele, Cristina Tudor, A. Pintea
The chlorophylls, carotenoids, total polyphenols contents and antioxidant activity in fresh and stored Parsley, celery and dill leaves were comparatively determined by spectrophotometric and high performance liquid chromatography (HPLC) methods. Results indicated that freezing does not induce significant changes in chlorophyll concentration. Drying induces significant decreases, varying according to the analyzed plant; parsley (60%), followed by dill (56.4%) and celery (45.3%). The carotenoids identified in all plants were lutein, b-carotene and cis-β-carotene. Fresh parsley leaves showed the highest total carotenoid concentration (21.64 mg/100g FW), followed by celery (15.41 mg/100g FW) and dill (14.95 mg/100g FW). Total polyphenols had an average concentration of 2.15 mg/g FW in parsley; 2.7 mg/g FW in dill and almost the double, 4.13 mg/g FW in celery. Drying of leaves induced a decrease with approximately 50% of the total polyphenol concentration, while freezing, did not significantly affect it. The highest antioxidant activity was observed in the dill, followed by parsley and celery. In both types of methods (freezing and drying in the oven), decreases in antioxidant activity were observed.
{"title":"Bioactive Compounds and Antioxidant Activity in Some Fresh and Canned Aromatic Herbs","authors":"S. Andrei, A. Bunea, C. Bele, Cristina Tudor, A. Pintea","doi":"10.15835/BUASVMCN-FST:2018.0012","DOIUrl":"https://doi.org/10.15835/BUASVMCN-FST:2018.0012","url":null,"abstract":"The chlorophylls, carotenoids, total polyphenols contents and antioxidant activity in fresh and stored Parsley, celery and dill leaves were comparatively determined by spectrophotometric and high performance liquid chromatography (HPLC) methods. Results indicated that freezing does not induce significant changes in chlorophyll concentration. Drying induces significant decreases, varying according to the analyzed plant; parsley (60%), followed by dill (56.4%) and celery (45.3%). The carotenoids identified in all plants were lutein, b-carotene and cis-β-carotene. Fresh parsley leaves showed the highest total carotenoid concentration (21.64 mg/100g FW), followed by celery (15.41 mg/100g FW) and dill (14.95 mg/100g FW). Total polyphenols had an average concentration of 2.15 mg/g FW in parsley; 2.7 mg/g FW in dill and almost the double, 4.13 mg/g FW in celery. Drying of leaves induced a decrease with approximately 50% of the total polyphenol concentration, while freezing, did not significantly affect it. The highest antioxidant activity was observed in the dill, followed by parsley and celery. In both types of methods (freezing and drying in the oven), decreases in antioxidant activity were observed.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82776669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.15835/buasvmcn-fst:2018.0016
S. Bărăităreanu, D. Daneș, Marius Dan, M. Daneş
Chlamydophila abortus is an important pathogen of small ruminants, causing reproductive failure manifested through abortion during the last 2-3 weeks of gestation, stillbirth, or delivery of weak lambs or kids, and orchitis and seminal vesiculitis in males. Also, C. abortus is a zoonotic bacteria, involved in influenza-like illness, pneumonia and abortions sometimes with severe complications in humans. The aim of this article is to evaluate the immunological status to C. abortus of Romanian small ruminant populations, geographically isolated, whose spontaneous uncontrolled contact is excluded. This paper is also assessing the risk of humans exposure to contaminated animals and food. The immunological status of the investigatet small ruminants was evaluated using an ELISA commercial kit and the results were analysed in correlation with the history of vaccination and type of animal breeding (traditional/professional farms). According to these results, the exposure of C. abortus is still to consider in traditional breeding farms, but in professional herds the serological tools are useless to uncover the circulation of wild strains, once the immunoprofilactic programs has been implemented. The public health risk relate to the close contact with the infected sheep and goats, common event in the traditional breeding.
{"title":"Seroconversion in Romanian Small Ruminant Populations Exposed at Chlamydophila Abortus and the Risk to Public Health","authors":"S. Bărăităreanu, D. Daneș, Marius Dan, M. Daneş","doi":"10.15835/buasvmcn-fst:2018.0016","DOIUrl":"https://doi.org/10.15835/buasvmcn-fst:2018.0016","url":null,"abstract":"Chlamydophila abortus is an important pathogen of small ruminants, causing reproductive failure manifested through abortion during the last 2-3 weeks of gestation, stillbirth, or delivery of weak lambs or kids, and orchitis and seminal vesiculitis in males. Also, C. abortus is a zoonotic bacteria, involved in influenza-like illness, pneumonia and abortions sometimes with severe complications in humans. The aim of this article is to evaluate the immunological status to C. abortus of Romanian small ruminant populations, geographically isolated, whose spontaneous uncontrolled contact is excluded. This paper is also assessing the risk of humans exposure to contaminated animals and food. The immunological status of the investigatet small ruminants was evaluated using an ELISA commercial kit and the results were analysed in correlation with the history of vaccination and type of animal breeding (traditional/professional farms). According to these results, the exposure of C. abortus is still to consider in traditional breeding farms, but in professional herds the serological tools are useless to uncover the circulation of wild strains, once the immunoprofilactic programs has been implemented. The public health risk relate to the close contact with the infected sheep and goats, common event in the traditional breeding.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77240024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-14DOI: 10.15835/buasvmcn-fst:2017.0034
A. Nistor, L. Nistor, M. Usturoi
Egg is a rich source of nutrients, such as high-quality protein (containing the ideal proportion of amino acids necessary for human body development) and a rich variety of vitamins and minerals. Liquid egg products have grown to a large extent in the food industry and beyond, which is an important reason to know their quality. For the analysis was used fresh pasteurized yolk and white wrapped bag in box. The fatty acids were determined by gas chromatographic method and amino acids by high performance liquid chromatography method. The results indicated that 34% of total fatty acids analyzed are saturated fatty acids, the main constituent being palmitic acid (25%) and 66% are unsaturated acids and the main constituent is oleic acid (37%). The rate of essential amino acids which are in pasteurized liquid white represents 46% from total analyzed amino acids and in the case of yolk the share is 44%. From obtained data for fatty acids content our limits are higher for polyunsaturated acids than those indicated by other researchers, and for monounsaturated acids are lower than data obtain by other authors. Regarding the essential amino acids content, the obtained data have higher values than the ones obtain by other authors. A possible explanation for the fluctuations in the profile of fatty acids and amino acids could be the feed distributed to the birds from which the eggs are obtained.
{"title":"A Review of Fatty Acid and Amino Acids Profile from Pasteurized Egg Liquids Produced in Romania","authors":"A. Nistor, L. Nistor, M. Usturoi","doi":"10.15835/buasvmcn-fst:2017.0034","DOIUrl":"https://doi.org/10.15835/buasvmcn-fst:2017.0034","url":null,"abstract":"Egg is a rich source of nutrients, such as high-quality protein (containing the ideal proportion of amino acids necessary for human body development) and a rich variety of vitamins and minerals. Liquid egg products have grown to a large extent in the food industry and beyond, which is an important reason to know their quality. For the analysis was used fresh pasteurized yolk and white wrapped bag in box. The fatty acids were determined by gas chromatographic method and amino acids by high performance liquid chromatography method. The results indicated that 34% of total fatty acids analyzed are saturated fatty acids, the main constituent being palmitic acid (25%) and 66% are unsaturated acids and the main constituent is oleic acid (37%). The rate of essential amino acids which are in pasteurized liquid white represents 46% from total analyzed amino acids and in the case of yolk the share is 44%. From obtained data for fatty acids content our limits are higher for polyunsaturated acids than those indicated by other researchers, and for monounsaturated acids are lower than data obtain by other authors. Regarding the essential amino acids content, the obtained data have higher values than the ones obtain by other authors. A possible explanation for the fluctuations in the profile of fatty acids and amino acids could be the feed distributed to the birds from which the eggs are obtained.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86955988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-14DOI: 10.15835//BUASVMCN-FST:2017.0012
C. Predescu, C. Papuc, G. Ștefan, G. Goran
Plant extracts rich in phenolics and vegetal fermented juices rich in nitrite may be used as preservatives in the meat industry. The aim of this study was to evaluate the effects of hawthorn berry phenolics and nitrite from parsley roots fermented juice, on unsmoked frankfurters. Seven lots of frankfurters were prepared. Two lots were prepared using ascorbate and parsley juice nitrite (each preservative 50 ppm, L1; each preservative 25 ppm, L4), two lots were prepared using hawthorn berry phenolics and parsley juice nitrite (each preservative 50 ppm, L2; each preservative 25 ppm, L5), two lots were prepared only with parsley juice nitrite (50 ppm, L3; 25 ppm, L6), while L7, control lot, was prepared with 50 ppm ascorbate and 50 ppm synthetic nitrite. pH, thiobarbituric acid reactive substances, cure efficiency, residual nitrite, total viable count, Enterobacteriaceae and coliform bacteria were determined at 3-day intervals for 15 days. During storage, comparatively with control, the best physico-chemical parameters were found for L4 and L5 of frankfurters, while the best microbiological parameters were found for frankfurters treated with ascorbate and parsley juice nitrite (L1 and L4). The addition of natural antioxidants and nitrite was able to improve unsmoked frankfurters’ shelf-life.
{"title":"The Effect of Hawthorn Berry Phenolics and Parsley Roots Fermented Juice on Frankfurters’ Shelf-Life","authors":"C. Predescu, C. Papuc, G. Ștefan, G. Goran","doi":"10.15835//BUASVMCN-FST:2017.0012","DOIUrl":"https://doi.org/10.15835//BUASVMCN-FST:2017.0012","url":null,"abstract":"Plant extracts rich in phenolics and vegetal fermented juices rich in nitrite may be used as preservatives in the meat industry. The aim of this study was to evaluate the effects of hawthorn berry phenolics and nitrite from parsley roots fermented juice, on unsmoked frankfurters. Seven lots of frankfurters were prepared. Two lots were prepared using ascorbate and parsley juice nitrite (each preservative 50 ppm, L1; each preservative 25 ppm, L4), two lots were prepared using hawthorn berry phenolics and parsley juice nitrite (each preservative 50 ppm, L2; each preservative 25 ppm, L5), two lots were prepared only with parsley juice nitrite (50 ppm, L3; 25 ppm, L6), while L7, control lot, was prepared with 50 ppm ascorbate and 50 ppm synthetic nitrite. pH, thiobarbituric acid reactive substances, cure efficiency, residual nitrite, total viable count, Enterobacteriaceae and coliform bacteria were determined at 3-day intervals for 15 days. During storage, comparatively with control, the best physico-chemical parameters were found for L4 and L5 of frankfurters, while the best microbiological parameters were found for frankfurters treated with ascorbate and parsley juice nitrite (L1 and L4). The addition of natural antioxidants and nitrite was able to improve unsmoked frankfurters’ shelf-life.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83043569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-14DOI: 10.15835/buasvmcn-fst:2018.0009
Lavinia-Maria Chiş, D. Vodnar
An important factor in the detection of falsification is the control of the composition of the meat at each stage of manufacturing the product. The PCR method is based on the study of proteins and meat nucleic acids used in food for the detection of animal species. Another technique is the Elisa method that works on the principle of identification and measurement of the quantity of molecules in a sample. There are several types of Elisa to increase specificity due to differences in structure and sample characteristics. By comparing the two methods used to identify the processed meat product species, Real Time PCR had the highest prediction as results. However, the Elisa method is more time efficient and easier to use. Real Time PCR is effective in identifying processed meat products that require low detection. The Elisa Kit is useful because of the ease of use.
检测伪造的一个重要因素是在产品制造的每个阶段对肉类成分的控制。PCR方法是基于对食品中用于动物物种检测的蛋白质和肉类核酸的研究。另一种技术是Elisa法，它的工作原理是识别和测量样品中的分子数量。由于结构和样品特性的差异，有几种类型的Elisa可以提高特异性。通过比较两种用于肉制品品种鉴定的方法，Real Time PCR的预测结果最高。然而，Elisa法更省时，更容易使用。实时聚合酶链反应是有效的识别加工肉制品，需要低检测。Elisa试剂盒因易于使用而非常有用。
{"title":"Methods of Detecting Meat Species in Food of Animal Origin","authors":"Lavinia-Maria Chiş, D. Vodnar","doi":"10.15835/buasvmcn-fst:2018.0009","DOIUrl":"https://doi.org/10.15835/buasvmcn-fst:2018.0009","url":null,"abstract":"An important factor in the detection of falsification is the control of the composition of the meat at each stage of manufacturing the product. The PCR method is based on the study of proteins and meat nucleic acids used in food for the detection of animal species. Another technique is the Elisa method that works on the principle of identification and measurement of the quantity of molecules in a sample. There are several types of Elisa to increase specificity due to differences in structure and sample characteristics. By comparing the two methods used to identify the processed meat product species, Real Time PCR had the highest prediction as results. However, the Elisa method is more time efficient and easier to use. Real Time PCR is effective in identifying processed meat products that require low detection. The Elisa Kit is useful because of the ease of use.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74397235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-14DOI: 10.15835/buasvmcn-fst:2017.0038
Mǎdǎlina Nistor, Raluca Ghiman, H. Ayvaz, D. Rugină, D. Mada, A. Stǎnilǎ, C. Socaciu, Z. Diaconeasa
Red berries are important sources of bioactive compounds and they are known to provide unique health benefits. Lately, it has been proved that anthocyanins have health benefits against degenerative diseases such as cardiovascular disease, cancer or diabetes. Accordingly, the aim of this study was to characterize the anthocyanin content of anthocyanins pure extracts (APEs) obtained from raspberries (Rubus sp.) and mulberries (Morus sp.) and to evaluate their antiproliferative effect in vitro. Upon chromatographic analysis, three anthocyanins were identified in purified extracts of mulberries (M-APEs), with cyanidin-3-O-glucoside being more abundant. On the other hand, purified extracts of raspberries (R-APEs) contained 2 anthocyanins, both identified as cyanidin-derivatives. The in vitro test demonstrated that APEs decreased the proliferation on both HeLa and A2780 human cancer cell lines in a dose dependent manner, demonstrating that these two different berries are both rich sources of anthocyanins and are able to exert antiproliferative proprieties toward cervical and ovarian cancer.
红莓是生物活性化合物的重要来源，它们具有独特的健康益处。最近，花青素已被证明对心血管疾病、癌症或糖尿病等退行性疾病有健康益处。因此，本研究的目的是表征覆盆子(Rubus sp.)和桑葚(Morus sp.)中花青素纯提取物(APEs)的花青素含量，并评估其体外抗增殖作用。经色谱分析，在桑葚提取物中鉴定出3种花青素，其中花青素-3- o -葡萄糖苷含量较高。另一方面，纯化的树莓提取物(r - ape)含有2种花青素，均被鉴定为花青素衍生物。体外实验表明，类人猿对HeLa和A2780人癌细胞的增殖均呈剂量依赖性，表明这两种不同的浆果都是花青素的丰富来源，能够发挥对宫颈癌和卵巢癌的抗增殖特性。
{"title":"Antiproliferative Activity of Anthocyanins Pure Extracts from Mulberries and Raspberries on HeLa and A2780 Human Cancer Cell Lines","authors":"Mǎdǎlina Nistor, Raluca Ghiman, H. Ayvaz, D. Rugină, D. Mada, A. Stǎnilǎ, C. Socaciu, Z. Diaconeasa","doi":"10.15835/buasvmcn-fst:2017.0038","DOIUrl":"https://doi.org/10.15835/buasvmcn-fst:2017.0038","url":null,"abstract":"Red berries are important sources of bioactive compounds and they are known to provide unique health benefits. Lately, it has been proved that anthocyanins have health benefits against degenerative diseases such as cardiovascular disease, cancer or diabetes. Accordingly, the aim of this study was to characterize the anthocyanin content of anthocyanins pure extracts (APEs) obtained from raspberries (Rubus sp.) and mulberries (Morus sp.) and to evaluate their antiproliferative effect in vitro. Upon chromatographic analysis, three anthocyanins were identified in purified extracts of mulberries (M-APEs), with cyanidin-3-O-glucoside being more abundant. On the other hand, purified extracts of raspberries (R-APEs) contained 2 anthocyanins, both identified as cyanidin-derivatives. The in vitro test demonstrated that APEs decreased the proliferation on both HeLa and A2780 human cancer cell lines in a dose dependent manner, demonstrating that these two different berries are both rich sources of anthocyanins and are able to exert antiproliferative proprieties toward cervical and ovarian cancer.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73938495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-13DOI: 10.15835/buasvmcn-fst:2018.0004
A. Cioca, O. Heemken, L. Colobatiu, M. Mihaiu
In the last decade, studies have been focusing on determinations of persistent organic pollutants (POPs), well known for being involved in human chronic diseases. Various approaches and methodologies are used in monitoring these chemical substances in fish food chain. Therefore, their development, functionality and efficiency are vital for consumers’ protection. The aim of this paper is to review recently published analytical techniques for sample preparation in the determination of POP residues such as organochlorine compounds (OCs) and polychlorinated biphenyls (PCBs). Limitations and advantages of sample preparation techniques are discussed and compared so that they can facilitate the decision of future analysts upon adequate protocols in individual laboratories. A list of the most common compounds quantified nowadays is displayed. In addition, results achieved in original papers are used to briefly describe the current situation on different continents, with third world countries undergoing more pollution than the rest of the world.
{"title":"Methodologies and Results Concerning the Determination of Ocs And Pcbs From Fish Meat","authors":"A. Cioca, O. Heemken, L. Colobatiu, M. Mihaiu","doi":"10.15835/buasvmcn-fst:2018.0004","DOIUrl":"https://doi.org/10.15835/buasvmcn-fst:2018.0004","url":null,"abstract":"In the last decade, studies have been focusing on determinations of persistent organic pollutants (POPs), well known for being involved in human chronic diseases. Various approaches and methodologies are used in monitoring these chemical substances in fish food chain. Therefore, their development, functionality and efficiency are vital for consumers’ protection. The aim of this paper is to review recently published analytical techniques for sample preparation in the determination of POP residues such as organochlorine compounds (OCs) and polychlorinated biphenyls (PCBs). Limitations and advantages of sample preparation techniques are discussed and compared so that they can facilitate the decision of future analysts upon adequate protocols in individual laboratories. A list of the most common compounds quantified nowadays is displayed. In addition, results achieved in original papers are used to briefly describe the current situation on different continents, with third world countries undergoing more pollution than the rest of the world.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89330560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-13DOI: 10.15835/BUASVMCN-FST:2017.0020
L. Lipan, Lucía Sánchez Rodríguez, J. González, E. Sendra, F. Burló, F. Hernández, D. Vodnar, A. Barrachina
The great consumption of water and its scarcity in many areas of the world leads „irremediably” to accept that Spanish agriculture, like other Mediterranean agricultures, must adapt to the lack of this natural resource. Deficit irrigation (DI) strategies are routine practices used worldwide in order to increase net farm income by growing the crops effectiveness to use water. The importance of using this type of strategies is the fact that farmers have the opportunity not just to reduce the water consumption but also to obtain hydro sustainable products. Those are “theoretically” characterized by a high accumulation of secondary carbon metabolites in plant and a great accumulation of bioactive compounds in fruit, according to other studies already done in pistachio or table olives. As almond is the major nut crop in Mediterranean area different DI strategies are presented within this review together with their effect on the final product quality. Thus, the aim of the current review paper is to find all the necessary information about which DI strategies are the recommended to be applied in the almond crop in order to obtain high-quality fruits environmentally friendly. In addition, methods used to determine de quality and steps necessary to certify and protect this type of products are also presented.
{"title":"Sustainability of the Legal Endowments of Water in Almond Trees and a New Generation of High Quality Hydrosustainable Almonds","authors":"L. Lipan, Lucía Sánchez Rodríguez, J. González, E. Sendra, F. Burló, F. Hernández, D. Vodnar, A. Barrachina","doi":"10.15835/BUASVMCN-FST:2017.0020","DOIUrl":"https://doi.org/10.15835/BUASVMCN-FST:2017.0020","url":null,"abstract":"The great consumption of water and its scarcity in many areas of the world leads „irremediably” to accept that Spanish agriculture, like other Mediterranean agricultures, must adapt to the lack of this natural resource. Deficit irrigation (DI) strategies are routine practices used worldwide in order to increase net farm income by growing the crops effectiveness to use water. The importance of using this type of strategies is the fact that farmers have the opportunity not just to reduce the water consumption but also to obtain hydro sustainable products. Those are “theoretically” characterized by a high accumulation of secondary carbon metabolites in plant and a great accumulation of bioactive compounds in fruit, according to other studies already done in pistachio or table olives. As almond is the major nut crop in Mediterranean area different DI strategies are presented within this review together with their effect on the final product quality. Thus, the aim of the current review paper is to find all the necessary information about which DI strategies are the recommended to be applied in the almond crop in order to obtain high-quality fruits environmentally friendly. In addition, methods used to determine de quality and steps necessary to certify and protect this type of products are also presented.","PeriodicalId":44020,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2018-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88165593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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