Pub Date : 2019-11-01DOI: 10.1109/nanomed49242.2019.9130603
{"title":"Kimdaejung Convention Center","authors":"","doi":"10.1109/nanomed49242.2019.9130603","DOIUrl":"https://doi.org/10.1109/nanomed49242.2019.9130603","url":null,"abstract":"","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122341338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/NANOMED49242.2019.9130621
Md Yousuf Harun, M. A. Rahman, Joshua Mellinger, Willy Chang, Thomas T F Huang, B. Walker, Kristen Hori, A. Ohta
Automating human preimplantation embryo grading offers the potential for higher success rates with in vitro fertilization (IVF) by providing new quantitative and objective measures of embryo quality. Current IVF procedures typically use only qualitative manual grading, which is limited in the identification of genetically abnormal embryos. The automatic quantitative assessment of blastocyst expansion can potentially improve sustained pregnancy rates and reduce health risks from abnormal pregnancies through a more accurate identification of genetic abnormality. The expansion rate of a blastocyst is an important morphological feature to determine the quality of a developing embryo. In this work, a deep learning based human blastocyst image segmentation method is presented, with the goal of facilitating the challenging task of segmenting irregularly shaped blastocysts. The type of blastocysts evaluated here has undergone laser ablation of the zona pellucida, which is required prior to trophectoderm biopsy. This complicates the manual measurements of the expanded blastocyst's size, which shows a correlation with genetic abnormalities. The experimental results on the test set demonstrate segmentation greatly improves the accuracy of expansion measurements, resulting in up to 99.4% accuracy, 98.1% precision, 98.8% recall, a 98.4% Dice Coefficient, and a 96.9% Jaccard Index.
{"title":"Image Segmentation of Zona-Ablated Human Blastocysts","authors":"Md Yousuf Harun, M. A. Rahman, Joshua Mellinger, Willy Chang, Thomas T F Huang, B. Walker, Kristen Hori, A. Ohta","doi":"10.1109/NANOMED49242.2019.9130621","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130621","url":null,"abstract":"Automating human preimplantation embryo grading offers the potential for higher success rates with in vitro fertilization (IVF) by providing new quantitative and objective measures of embryo quality. Current IVF procedures typically use only qualitative manual grading, which is limited in the identification of genetically abnormal embryos. The automatic quantitative assessment of blastocyst expansion can potentially improve sustained pregnancy rates and reduce health risks from abnormal pregnancies through a more accurate identification of genetic abnormality. The expansion rate of a blastocyst is an important morphological feature to determine the quality of a developing embryo. In this work, a deep learning based human blastocyst image segmentation method is presented, with the goal of facilitating the challenging task of segmenting irregularly shaped blastocysts. The type of blastocysts evaluated here has undergone laser ablation of the zona pellucida, which is required prior to trophectoderm biopsy. This complicates the manual measurements of the expanded blastocyst's size, which shows a correlation with genetic abnormalities. The experimental results on the test set demonstrate segmentation greatly improves the accuracy of expansion measurements, resulting in up to 99.4% accuracy, 98.1% precision, 98.8% recall, a 98.4% Dice Coefficient, and a 96.9% Jaccard Index.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132756423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We propose a new framework of tracking nanoswimmers for cancer detection and targeted drug delivery in microscale scenarios. The framework includes a novel multimodal complex vascular topological model to emulate the real vasculature inside the human body, and an efficient system model that is able to track nanoswimmers in an in vivo environment. The multimodal vascular model consists of three consecutive subnetworks to represent normal arteries, normal subcutaneous capillaries and tumor vasculature, aiming for setting up the trajectories towards tumors. The proposed tracking system model, based on Kalman filters, very significantly decreases the noise induced by a variety of sources by approximately 51.3%-75% to provide accurate position information of the nanoswimmers.
{"title":"System Model for Tracking In Vivo Nanoswimmers Using Kalman Filter for Nanobiomedicine","authors":"Zheng Gong, Yifan Chen, Shaolong Shi, Xiaoyou Lin, M. Cree, Neda Sharifi","doi":"10.1109/NANOMED49242.2019.9130610","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130610","url":null,"abstract":"We propose a new framework of tracking nanoswimmers for cancer detection and targeted drug delivery in microscale scenarios. The framework includes a novel multimodal complex vascular topological model to emulate the real vasculature inside the human body, and an efficient system model that is able to track nanoswimmers in an in vivo environment. The multimodal vascular model consists of three consecutive subnetworks to represent normal arteries, normal subcutaneous capillaries and tumor vasculature, aiming for setting up the trajectories towards tumors. The proposed tracking system model, based on Kalman filters, very significantly decreases the noise induced by a variety of sources by approximately 51.3%-75% to provide accurate position information of the nanoswimmers.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121461834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/NANOMED49242.2019.9130624
S. B. Alvi, S. Paradkar, Arpan Pradhan, R. Srivastava, A. Rengan
Photothermal therapy (PTT) is one of the emerging modalities for the treatment of cancer. Among various near Infrared (NIR) responsive nanomaterials which are under research, gold coated liposomes (Au Lipos NPs) are considered as a promising candidate for photothermal therapy and triggered drug delivery agent. The external NIR light trigger is advantageous because the trigger itself causes therapeutic effect (by inducing localized hyperthermia). In the current study we have investigated the timing of therapeutic trigger that governs the therapeutic efficacy. We found a significant enhancement in therapy when early trigger was applied. Moreover, the curcumin (hydrophobic model drug) entrapped in Au Lipos Cur NPs acted as a thermal sensitizer further enhancing the therapeutic outcome. The in vivo repeated dose administration in rats revealed no specific toxicity to the animal.
光热疗法(PTT)是一种新兴的癌症治疗方法。在各种正在研究的近红外响应纳米材料中，金包覆脂质体(Au Lipos NPs)被认为是光热治疗和触发药物递送剂的有前途的候选材料。外部近红外光触发是有利的，因为触发本身引起治疗效果(通过诱导局部热疗)。在目前的研究中，我们研究了控制治疗效果的治疗触发时间。我们发现早期触发的治疗效果显著增强。此外，包裹在Au lippos Cur NPs中的姜黄素(疏水模型药物)作为热敏剂进一步提高了治疗效果。大鼠体内重复给药对动物无特异性毒性。
{"title":"Timing The Therapeutic Trigger of Au Lipos Cur NPs for Effective Photothermal Therapy","authors":"S. B. Alvi, S. Paradkar, Arpan Pradhan, R. Srivastava, A. Rengan","doi":"10.1109/NANOMED49242.2019.9130624","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130624","url":null,"abstract":"Photothermal therapy (PTT) is one of the emerging modalities for the treatment of cancer. Among various near Infrared (NIR) responsive nanomaterials which are under research, gold coated liposomes (Au Lipos NPs) are considered as a promising candidate for photothermal therapy and triggered drug delivery agent. The external NIR light trigger is advantageous because the trigger itself causes therapeutic effect (by inducing localized hyperthermia). In the current study we have investigated the timing of therapeutic trigger that governs the therapeutic efficacy. We found a significant enhancement in therapy when early trigger was applied. Moreover, the curcumin (hydrophobic model drug) entrapped in Au Lipos Cur NPs acted as a thermal sensitizer further enhancing the therapeutic outcome. The in vivo repeated dose administration in rats revealed no specific toxicity to the animal.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134454628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/NANOMED49242.2019.9130618
Md Yousuf Harun, Thomas T F Huang, A. Ohta
Embryo quality assessment based on morphological attributes is important for achieving higher pregnancy rates from in vitro fertilization (IVF). The accurate segmentation of the embryo's inner cell mass (ICM) and trophectoderm epithelium (TE) is important, as these parameters can help to predict the embryo viability and live birth potential. However, segmentation of the ICM and TE is difficult due to variations in their shape and similarities in their textures, both with each other and with their surroundings. To tackle this problem, a deep neural network (DNN) based segmentation approach was implemented. The DNN can identify the ICM region with 99.1% accuracy, 94.9% precision, 93.8% recall, a 94.3% Dice Coefficient, and a 89.3% Jaccard Index. It can extract the TE region with 98.3% accuracy, 91.8% precision, 93.2% recall, a 92.5% Dice Coefficient, and a 85.3% Jaccard Index.
{"title":"Inner Cell Mass and Trophectoderm Segmentation in Human Blastocyst Images using Deep Neural Network","authors":"Md Yousuf Harun, Thomas T F Huang, A. Ohta","doi":"10.1109/NANOMED49242.2019.9130618","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130618","url":null,"abstract":"Embryo quality assessment based on morphological attributes is important for achieving higher pregnancy rates from in vitro fertilization (IVF). The accurate segmentation of the embryo's inner cell mass (ICM) and trophectoderm epithelium (TE) is important, as these parameters can help to predict the embryo viability and live birth potential. However, segmentation of the ICM and TE is difficult due to variations in their shape and similarities in their textures, both with each other and with their surroundings. To tackle this problem, a deep neural network (DNN) based segmentation approach was implemented. The DNN can identify the ICM region with 99.1% accuracy, 94.9% precision, 93.8% recall, a 94.3% Dice Coefficient, and a 89.3% Jaccard Index. It can extract the TE region with 98.3% accuracy, 91.8% precision, 93.2% recall, a 92.5% Dice Coefficient, and a 85.3% Jaccard Index.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115816210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/NANOMED49242.2019.9130604
Chun-Chih Yeh, Andrew Goh, K. Lei
Metastasis is a serious disease caused by primary tumor cell dissemination to different organs. Although, invasion of primary tumor cell is the initial step required in tumor metastasis, migration is also the important key elements activity in cancer cell detach from the primary tumor and spreading to other extracellular matrix. Currently, various cell migration assays have been developed to investigate the motility behavior of the multicellular, including wound healing / scratch assay and transwell assay. However, most of the in vitro researches of cell motility are based on two-dimensional (2D) culture systems, which limit our understanding of the mechanisms of cell motility. Thus, three dimensional culture (3D) model is necessary which is able to mimic conditions and microenvironments of in vivo. In this study, a microfluidic chip was developed and matrigel was used as cell scaffold. In order to perform 3D cell migration assay, cells cultured in 3D environment and invaded through a matrigel filled microchannel. In addition, we determine the motility of the cancer cell correlated to the interleukin 6 (IL-6) concentration. Here, we successfully developed a prototype of visualizing and quantifying 3D cell migration assay.
{"title":"Development of a Microfluidic Chip for 3D Cancer Cell Migration Assay","authors":"Chun-Chih Yeh, Andrew Goh, K. Lei","doi":"10.1109/NANOMED49242.2019.9130604","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130604","url":null,"abstract":"Metastasis is a serious disease caused by primary tumor cell dissemination to different organs. Although, invasion of primary tumor cell is the initial step required in tumor metastasis, migration is also the important key elements activity in cancer cell detach from the primary tumor and spreading to other extracellular matrix. Currently, various cell migration assays have been developed to investigate the motility behavior of the multicellular, including wound healing / scratch assay and transwell assay. However, most of the in vitro researches of cell motility are based on two-dimensional (2D) culture systems, which limit our understanding of the mechanisms of cell motility. Thus, three dimensional culture (3D) model is necessary which is able to mimic conditions and microenvironments of in vivo. In this study, a microfluidic chip was developed and matrigel was used as cell scaffold. In order to perform 3D cell migration assay, cells cultured in 3D environment and invaded through a matrigel filled microchannel. In addition, we determine the motility of the cancer cell correlated to the interleukin 6 (IL-6) concentration. Here, we successfully developed a prototype of visualizing and quantifying 3D cell migration assay.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125286757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/NANOMED49242.2019.9130613
V. Sirdeshmukh, Indrayani S. Kadu, Shreshtha S. Mishra, Anup A. Kale
Hydrogen Peroxide (H2O2) is a well-known reactive oxygen species produced in various biological phenomena. In various pathological and physiological conditions, higher concentrations of H2O2 can cause lipid peroxidation, DNA base modification, protein degradation, etc. H2O2 present in quantities of about $20-50 mumathrm{M}$ or more can have deleterious effects as it can easily dissolve in aqueous solutions and penetrate biological membranes easily. Hence, sensitive and selective detection of H2O2is important under physiological conditions. In this work, we report a non-enzymatic electrochemical method for the facile and sensitive detection of H2O2. We explored the approach of using polymer-nanocomposite as an efficient transducer platform. We synthesized three-phase composite polymer system of PEDOT:PSS/GO/MnO2, comprising of poly(3,4-ethylenedioxythiophene): poly(styrene-sulfonate) (PEDOT:PSS), graphene oxide (GO) and manganese dioxide (MnO2). On being exposed to H2O2, MnO2 undergoes a redox reaction which is responsible for the sensing ability of this material. The composite was prepared using the conventional solution mixing method and then used to modify screen printed electrodes. The physicochemical characterization was carried out by Raman Spectroscopy and Fourier Transform Infrared Spectroscopy. Cyclic Voltammetry results showed that the nanocomposite showed high electrochemical activity for the detection of hydrogen peroxide (H2O2) in alkaline medium. The PEDOT:PSS/GO/MnO2 based electrode exhibits high sensitivity and selectivity for electrochemical detection of H2O2 with a sensitivity of $0.5mu mathrm{M}$. The present study demonstrates the promising ability of this novel nanocomposite for fabrication of non-enzymatic H2O2 biosensors. This method can further be explored for point-of-care detection.
过氧化氢(H2O2)是在各种生物现象中产生的一种众所周知的活性氧。在各种病理生理条件下，高浓度的H2O2可引起脂质过氧化、DNA碱基修饰、蛋白质降解等。H2O2的存在量约为$20-50 mumathrm{M}$或更多，因为它很容易溶解在水溶液中并很容易穿透生物膜，因此会产生有害影响。因此，在生理条件下对h2o2进行灵敏、选择性的检测是非常重要的。在这项工作中，我们报告了一种简便灵敏的非酶电化学检测H2O2的方法。我们探索了使用聚合物-纳米复合材料作为高效换能器平台的方法。合成了由聚(3,4-乙烯二氧噻吩)、聚(苯乙烯磺酸盐)(PEDOT:PSS)、氧化石墨烯(GO)和二氧化锰(MnO2)组成的PEDOT:PSS/GO/MnO2三相复合聚合物体系。当暴露于H2O2时，MnO2发生氧化还原反应，这是该材料传感能力的原因。采用常规溶液混合法制备该复合材料，并将其用于丝网印刷电极的修饰。利用拉曼光谱和傅里叶变换红外光谱对其进行了理化表征。循环伏安法结果表明，该纳米复合材料对碱性介质中过氧化氢(H2O2)的检测具有较高的电化学活性。PEDOT:PSS/GO/MnO2基电极对H2O2的电化学检测具有很高的灵敏度和选择性，灵敏度为0.5mu math {M}$。目前的研究表明，这种新型纳米复合材料具有制造非酶H2O2生物传感器的良好能力。这种方法可以进一步探索点护理检测。
{"title":"A Novel Non-Enzymatic PEDOT:PSS/GO/MnO2 Based Biosensor For Hydrogen Peroxide Detection in Biological Samples","authors":"V. Sirdeshmukh, Indrayani S. Kadu, Shreshtha S. Mishra, Anup A. Kale","doi":"10.1109/NANOMED49242.2019.9130613","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130613","url":null,"abstract":"Hydrogen Peroxide (H<inf>2</inf>O<inf>2</inf>) is a well-known reactive oxygen species produced in various biological phenomena. In various pathological and physiological conditions, higher concentrations of H<inf>2</inf>O<inf>2</inf> can cause lipid peroxidation, DNA base modification, protein degradation, etc. H<inf>2</inf>O<inf>2</inf> present in quantities of about <tex>$20-50 mumathrm{M}$</tex> or more can have deleterious effects as it can easily dissolve in aqueous solutions and penetrate biological membranes easily. Hence, sensitive and selective detection of H<inf>2</inf>O<inf>2</inf>is important under physiological conditions. In this work, we report a non-enzymatic electrochemical method for the facile and sensitive detection of H<inf>2</inf>O<inf>2</inf>. We explored the approach of using polymer-nanocomposite as an efficient transducer platform. We synthesized three-phase composite polymer system of PEDOT:PSS/GO/MnO<inf>2</inf>, comprising of poly(3,4-ethylenedioxythiophene): poly(styrene-sulfonate) (PEDOT:PSS), graphene oxide (GO) and manganese dioxide (MnO<inf>2</inf>). On being exposed to H<inf>2</inf>O<inf>2</inf>, MnO<inf>2</inf> undergoes a redox reaction which is responsible for the sensing ability of this material. The composite was prepared using the conventional solution mixing method and then used to modify screen printed electrodes. The physicochemical characterization was carried out by Raman Spectroscopy and Fourier Transform Infrared Spectroscopy. Cyclic Voltammetry results showed that the nanocomposite showed high electrochemical activity for the detection of hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>) in alkaline medium. The PEDOT:PSS/GO/MnO<inf>2</inf> based electrode exhibits high sensitivity and selectivity for electrochemical detection of H<inf>2</inf>O<inf>2</inf> with a sensitivity of <tex>$0.5mu mathrm{M}$</tex>. The present study demonstrates the promising ability of this novel nanocomposite for fabrication of non-enzymatic H<inf>2</inf>O<inf>2</inf> biosensors. This method can further be explored for point-of-care detection.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129851665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/NANOMED49242.2019.9130623
Chun-Hao Huang, K. Lei
Cancer cells possess a broad spectrum of invasion mechanisms. Quantitative analysis of cancer cell invasion process under tested condition is important to precisely study the cellular invasion capability. In this study, a microfluidic device was developed and electrodes were embedded in the microchannel for the impedimetric measurement of cell invasion. Cancer cells were stimulated by interleukin-6 cytokine and invaded along the hydrogel-filled microchannel. The three-dimensional (3D) cell invasion process was monitored by measuring the impedance across the electrodes. The cell invasion speed could be calculated based on the cell invasion distance divided by time. Thus, 3D cell invasion process was demonstrated to be quantitatively monitored in real-time and non-invasive manner. The current development provides a promising and quantitative tool for cell invasion assay.
{"title":"Real-Time and Non-invasive Measurement of 3D Cancer Cell Invasion Process under IL-6 Cytokine Stimulation","authors":"Chun-Hao Huang, K. Lei","doi":"10.1109/NANOMED49242.2019.9130623","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130623","url":null,"abstract":"Cancer cells possess a broad spectrum of invasion mechanisms. Quantitative analysis of cancer cell invasion process under tested condition is important to precisely study the cellular invasion capability. In this study, a microfluidic device was developed and electrodes were embedded in the microchannel for the impedimetric measurement of cell invasion. Cancer cells were stimulated by interleukin-6 cytokine and invaded along the hydrogel-filled microchannel. The three-dimensional (3D) cell invasion process was monitored by measuring the impedance across the electrodes. The cell invasion speed could be calculated based on the cell invasion distance divided by time. Thus, 3D cell invasion process was demonstrated to be quantitatively monitored in real-time and non-invasive manner. The current development provides a promising and quantitative tool for cell invasion assay.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132393239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We demonstrate a novel minimally invasive flexible sensor for continuous in vivo glucose monitoring. This sensor is based on a gold-coated polyimide microneedle array (MNA) electrode, which is modified with glucose oxidase. Owing to the spiky micro-needles, flexible substrate, and biocompatible materials, the enzymatic surface is able to penetrate the outer skin, and directly detect the subcutaneous glucose with high biosafety. The current results show that our device performs good response, linearity, and stability to the change of glucose concentration.
{"title":"A Minimally Invasive Flexible Micro-Needle Array as Continuous in vivo Electrochemical Glucose Sensor","authors":"Qun Mou, Junshi Li, Fengyi Zheng, Yue Cui, Yufeng Jin, Zhihong Li","doi":"10.1109/NANOMED49242.2019.9130616","DOIUrl":"https://doi.org/10.1109/NANOMED49242.2019.9130616","url":null,"abstract":"We demonstrate a novel minimally invasive flexible sensor for continuous in vivo glucose monitoring. This sensor is based on a gold-coated polyimide microneedle array (MNA) electrode, which is modified with glucose oxidase. Owing to the spiky micro-needles, flexible substrate, and biocompatible materials, the enzymatic surface is able to penetrate the outer skin, and directly detect the subcutaneous glucose with high biosafety. The current results show that our device performs good response, linearity, and stability to the change of glucose concentration.","PeriodicalId":443566,"journal":{"name":"2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED)","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122094332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/NANOMED49242.2019.9130612
Asma Wasfi, F. Awwad
DNA base detection is a vastly advancing technology to obtain the bases sequence in human genome thus allowing for recognition and medication of disease. Acquiring reliable, quick, and cheap DNA sequencing facilitates personalized medicine procedure where right medication will be given to patients. In this article, a semi-empirical model is presented for calculating electron transport properties for the z-shaped sensor to identify the DNA sequence. The z-shaped sensor is made of two metallic electrodes of zigzag graphene nanoribbon (ZGNR) connected through a semiconducting channel with a pore in the middle where DNA bases are translocated. The channel is made of armchair graphene nanoribbon (AGNR) which is semiconducting. Semi-empirical model and non-equilibrium Green's function are utilized to ananlyze the various electronic characteristics. The semi-empirical model used is an expansion of the extended Hückel technique with self-consistent Hartree potential. Using the non-equilibrium Green's function combined with self-consistent extended Hückel (NEGF+SC-EH), we show that each of the bases placed within the pore whose edge carbon atoms are passivated with nitrogen leads to a unique current. Several electronic properties are studied such as electrical current and transmission spectrum of DNA bases within the sensor's nanopore. These characteristics are investigated with modification of base orientation. Our study produced unique current for each of the DNA bases inside the pore.
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