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Exploring The Potency of Microalgae-Based Biofertilizer and Its Impact on Oil Palm Seedlings Growth 微藻基生物肥料的潜力及其对油棕幼苗生长的影响
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v44i1.3102
I. Sani, Yudistira Wahyu Kurnia, Hana Christine Sinthya, Richard Anthony, E. C. Situmorang, C. Utomo, T. Liwang
Indonesia is a major producer of palm oil. Consequently, the use of chemical fertilizers has become more extensive. Microalgae represent a potential alternative for enhancing and protecting crops based on their cell elements. This study applies dry biomass or liquid culture formulation of the green microalgae Haematococcus pluvialis to the rhizosphere of oil palm pre-nursery as a biofertilizer. Soil application of microalgae biomass of 0.5 g/l (MA) or liquid culture of 10% (v/v) (BCMA) is carried out to assess its effects on 4-months-old oil palm at the nursery stage. The compatibility test between microalgae and bio fungicide agents in agricultural practices, Trichoderma spp., is also tested on both microalgae formulations. The result shows that both microalgae biomass and liquid culture, alone or combined with Trichoderma spp., give a better growth performance to the oil palm. The application of MA and BCMA result in a maximum increment of plant height, leaves count, and chlorophyll content. Furthermore, the application of BCMA gives better oil palm growth performance, which may probably be influenced by the accessibility of nutrients for microalgae growth. The study reveals that soil application of microalgae as biofertilizers can improve oil palm growth performance.
印度尼西亚是棕榈油的主要生产国。因此,化肥的使用变得更加广泛。微藻是一种基于其细胞成分增强和保护作物的潜在替代品。本研究将绿色微藻雨生红球藻的干生物量或液体培养配方作为生物肥料应用于油棕苗圃前的根际。在土壤中施用0.5g/l微藻生物量(MA)或10%(v/v)液体培养物(BCMA),以评估其在4个月大油棕育苗期的效果。微藻和农业实践中的生物杀菌剂木霉之间的兼容性测试也在两种微藻制剂上进行了测试。结果表明,无论是微藻生物量还是液体培养,单独或与木霉联合培养,都能使油棕具有更好的生长性能。MA和BCMA的应用导致株高、叶片数和叶绿素含量的最大增加。此外,BCMA的应用提供了更好的油棕生长性能,这可能受到微藻生长所需营养物质的可及性的影响。研究表明,微藻作为生物肥料在土壤中的应用可以提高油棕的生长性能。
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引用次数: 1
The Yield Stability and Adaptability of Bambara Groundnut at Three Locations 三地班巴拉花生产量稳定性及适应性研究
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v44i1.3079
Gita Novita Sari, D. Saptadi, K. Kuswanto
Varieties with high yield stability are required to increase the yield. This study examines the strength and adaptability of seven Bambara groundnut lines in three areas. The seven lines used are CCC 1.6, PWBG 6, PWBG 521, SS 342, SS 242, BBL 11, and TVSU 86 as checks. The research sites are Brawijaya University Experimental Station, Farmer field in Madiun and Indonesia Legumes, and Tuber Crop Research Institute (ILETRI) Research Station. Research is conducted from February to October 2020. The study used a randomized block design with three replications. The Eberhart-Russel and FinlayWilkinson methods were used to analyze stability and adaptability. The Genotype x Environmental interaction (GxE) results of the 7 Bambara groundnut lines are at 50% flowering time, seed weight per plant, 100-seed weight, yield, and harvest age. The stability and adaptability analysis shows that BBL 1.1 line is the variety with an earlier harvest period, highest yield potential, good stability, and wide adaptability. The CCC 1.1, PWBG 6, PWBG 5.2.1, and SS 2.4.2 production lines are stable in all experimental environments but low productivity. The SS 3.4.2 is suitable for planting in a production environment. TVSU 86 is ideal for producing in marginal habitats such as drought conditions.
需要产量稳定性高的品种来提高产量。本研究考察了七个斑巴拉花生品系在三个地区的强度和适应性。使用的七条线路是CCC 1.6、PWBG 6、PWBG 521、SS 342、SS 242、BBL 11和TVSU 86作为检查。研究地点为Brawijaya大学实验站、Madiun和Indonesia Legumes的农田以及块茎作物研究所(ILETRI)研究站。研究于2020年2月至10月进行。该研究采用了三次重复的随机分组设计。采用Eberhart-Russel和Finlay-Wilkinson方法分析稳定性和适应性。7个班巴拉花生品系的基因型x环境相互作用(GxE)结果为50%开花时间、单株种子重量、100种子重量、产量和收获年龄。稳定性和适应性分析表明,BBL1.1系是收获期早、产量潜力大、稳定性好、适应性广的品种。CCC 1.1、PWBG 6、PWBG 5.2.1和SS 2.4.2生产线在所有实验环境中都是稳定的,但生产率较低。SS 3.4.2适合在生产环境中种植。TVSU 86是在干旱等边缘栖息地生产的理想选择。
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引用次数: 2
Pathogenicity of Sclerotium rolfsii Isolates Causing Stem and Root Rot Disease of Cowpea Vigna unguiculata (L.) Walp and Management Using Trichoderma Species 豇豆根茎腐病病原菌罗氏菌核菌分离株的致病性木霉的种类及其管理
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v44i1.3372
Victor Ohileobo Dania, Enwongu Uso Henry
Sclerotium rolfsii is a soil-borne pathogen causing stem and root rot disease with concomitant reduction in growth and yield of cowpea. Therefore, this study evaluated pathogenicity of S. rolfsii isolates and the management of root and stem rot disease using Trichoderma species. The isolates were obtained from cowpea rhizosphere and pathogenicity was determined using a susceptible cowpea genotype ITI0K–815-5 in an experiment arranged in a completely randomized design with four replications. In vitro and in vivo trials of Trichoderma spp. against a selected virulent isolate Sclerotium rolfsii (SR06) were conducted in the laboratory and screenhouse, respectively. All nine S. rolfsii isolates were pathogenic to the cowpea genotype, while isolate SR 06 was the most virulent with 85.56% infectivity rate. Treatments with T. virens and T. atroviride application reduced the disease incidences to 22.2% and 25.3%, respectively, compared to synthetic funguforce fungicide. Plants that were inoculated and treated with T. atroviride had significantly higher yield of 435 kg/ha than other treatments (p<0.037). Findings from this research encourages the incorporation of Trichoderma species in the integrated management of S. rolfsii pathogen and the incidence of stem and root rot disease in cowpea.
油菜菌核病是一种土传病原体,可引起豇豆茎根腐烂病,并伴随着生长和产量的下降。因此,本研究使用木霉属物种评估了S.rolfsii分离株的致病性和根腐病和茎腐病的管理。分离株是从豇豆根际获得的,在一个完全随机设计的实验中,使用敏感的豇豆基因型ITI0K–815-5确定了致病性,共有四个重复。在实验室和筛选室分别对木霉(Trichoderma spp.)对选定的强毒分离株硬核菌(SR06)进行了体外和体内试验。9个罗夫氏乳杆菌分离株均对豇豆基因型具有致病性,SR06株毒力最强,感染率为85.56%。与合成真菌力杀菌剂相比,应用T.virens和T.atrovirid处理的疾病发生率分别降低到22.2%和25.3%。与其他处理相比,接种和处理阿特罗维里的植物产量显著提高,达到435公斤/公顷(p<0.037)。这项研究的结果鼓励将木霉属物种纳入对S.rolfsii病原体的综合管理中,并降低豇豆茎根腐病的发病率。
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引用次数: 2
Various pH Media Influence Production of Pseudomonas fluorescens P20 Raw Secondary Metabolites for Controlling Damping-off (Pythium sp.) in Cucumber Seedlings 不同pH培养基对黄瓜幼苗荧光假单胞菌P20次级代谢产物的影响
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v44i1.2730
L. Soesanto, S. Latifah, Abdul Manan, Endang Mugiastuti, W. S. Suharti
This research aims to determine the best pH media for the production of Pseudomonas fluorescens P20 raw secondary metabolites, its effect on controlling damping-off, and on cucumber seedling growth. In vitro test uses completely randomized design with four replicates and seven treatments consisted of pH 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; and 8.0. In planta test uses a randomized block design with three replicates and ten treatments consisting of control, mancozeb 80%, and raw secondary metabolites with pH 7.0 and 7.5, and 4 concentration levels, i.e., 5, 10, 15, and 20%. Variables observe population density, inhibition ability, protease and chitinase qualitatively, germination ability, incubation period, disease incidence, the area under disease progress curve (AUDPC), crop height, number of leaves, root length, and crop fresh weight. The result shows that the best pH for the production of raw secondary metabolites is 7.0, indicated by population density as 5.68 × 1024 cfu/ml, inhibition ability as 50.8%, and the best protease and chitinase qualitatively. Application of the secondary metabolites on pH 7.0 could suppress disease intensity, incubation period, and AUDPC as 66.67, 77%, and 0%-day, respectively, and increase crop height, the number of leaves, root length, and crop fresh weight as 57.65, 37.19, 63, and 74%, respectively.
本研究旨在确定产生荧光假单胞菌P20原始次级代谢产物的最佳pH培养基,其对控制衰减和黄瓜幼苗生长的影响。体外试验采用完全随机设计,有四个重复和七个处理,包括pH 5.0;5.5;6.0;6.5;7.0;7.5;和8.0。植物体内试验采用随机区组设计,包括三个重复和十个处理,包括对照、80%代森锰锌和pH 7.0和7.5的原始次级代谢产物,以及4个浓度水平,即5%、10%、15%和20%。变量观察种群密度、抑制能力、蛋白酶和几丁质酶、发芽能力、潜伏期、发病率、疾病进展曲线下面积(AUDPC)、作物高度、叶数、根长和作物鲜重。结果表明,产生粗次生代谢产物的最佳pH为7.0,种群密度为5.68×1024cfu/ml,抑制能力为50.8%,蛋白酶和几丁质酶的质量最佳。在pH7.0条件下施用次级代谢产物可抑制病害强度、潜伏期和AUDPC,分别为66.67、77%和0%-天,并使作物高度、叶数、根长和鲜重分别增加57.65、37.19、63和74%。
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引用次数: 0
The Effect of Soil Submersion and Conditioner Materials on Residual Organophosphate Pesticides in Soil and Shallot Bulbs 土壤浸没和调理剂对土壤和葱球茎中有机磷农药残留的影响
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v41i0.1291
Ubad Badrudin, Syakiroh Jazilah, B. Prakoso
Soil health decreases and residual pesticides increase due to the application of inorganic fertilizers and pesticides continuously during the cultivation of crops. The effect of 12 hours or 24 hours soil submersion and chicken litters or zeolite application before planting on residual pesticides in soil and bulbs of shallots (Allium ascalonicum L.) are studied. Samples of soils and bulbs have proceeded after shallot cultivation conducted in Brebes, Indonesia. Then organophosphate residues in the samples are analyzed using gas chromatography in the Laboratory of the Indonesian Agricultural Environment Research Institute, Bogor. The data are compared to the standard of maximum residue levels (MRL) of pesticides in agricultural products. Results show that residual pesticides in treated soils are below the detection limit of the GC equipment, except malathion is detected with values ranging from 0.039-0.050 ppm. However, residual organophosphate pesticides in the bulbs are mainly below the maximum residue levels. The only exception is chlorpyrifos which has a value above the maximum residue levels of pesticides (0.076 ppm). Farmers should be educated in integrated pest management and applying synthetic pesticides as the last option for controlling pests and diseases.
在作物种植过程中,由于不断施用无机肥料和农药,导致土壤健康下降,农药残留增加。研究了栽植前土壤浸水12 h和24 h以及施用鸡凋落物或沸石对葱(Allium ascalonicum L.)土壤和鳞茎残留农药的影响。在印度尼西亚布里布进行了大葱种植后,进行了土壤和鳞茎的取样。然后在印尼农业环境研究所茂物实验室使用气相色谱法分析样品中的有机磷残留物。这些数据与农产品中农药的最大残留水平(MRL)标准进行了比较。结果表明,除马拉硫磷在0.039 ~ 0.050 ppm范围内检出外,处理后土壤中农药残留均低于气相色谱设备的检出限。但球茎中有机磷农药残留量基本低于最大残留量。唯一的例外是毒死蜱,其值高于农药的最大残留水平(0.076 ppm)。应教育农民进行病虫害综合管理,并将使用合成农药作为控制病虫害的最后选择。
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引用次数: 2
Sensitivity in Callus Tissue of Plectranthus amboinicus (L.) through Mutation Induction with Colchicine 秋水仙碱诱变对羊草愈伤组织的敏感性
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v44i1.3058
Kunto Wibisono, S. Aisyah, W. Nurcholis, S. Suhesti
Mutation induction by colchicine is one method to improve plant genetic diversity (polyploidy). This study aims to 1) obtain growth regulators composition suitable in inducing callus, 2) obtain the LC50 value in callus of P. amboinicus, 3) to determine the effect of colchicine on callus growth, 4) to get shoots (putative mutant) varian, regenerating from indirect organogenesis (callus) in MV1. The study is conducted at the Laboratory of Agricultural Seed Management, Plantation Research, and Development Center from June 2018 to June 2019. The study uses a complete randomized design with four treatments and three replications. Explants use ware the nodes. Callus induction uses MS0 medium with 0.5 ppm 2.4-D, 2 ppm 2.4-D, 0.5 ppm 2.4-D + 1 ppm BAP, and 0.5 ppm 2.4-D + 0.5 ppm TDZ treatment. Mutation induction was performed using colchicine concentration at 0%, 0.02%, 0.04%, and 0.06% treatments. The regeneration media is an MS0 medium. The results show the best combination of plant growth regulators to induce the callus is 0.5 ppm 2.4-D. The LC50 colchicine concentration is at 0.0275%. However, this phenotype is reversible. An additional subculture period is required to move to normal morphology
秋水仙碱诱变是提高植物遗传多样性(多倍体)的一种方法。本研究的目的是:1)获得适合于诱导愈伤组织的生长调节剂组合物;2)获得安氏乳杆菌愈伤组织中的LC50值;3)测定秋水仙碱对愈伤组织生长的影响;4)在MV1中获得由间接器官发生(愈伤组织)再生的芽(推定突变体)变体。该研究于2018年6月至2019年6月在农业种子管理、种植研究与发展中心实验室进行。该研究采用了完全随机设计,共有四种治疗方法和三次重复。外植体使用ware节点。愈伤组织诱导使用MS0培养基,其具有0.5ppm 2.4-D、2ppm 2.4-D、0.5ppm 2.4-D+1ppm BAP和0.5ppm 2.4-D-0.5ppm TDZ处理。使用浓度为0%、0.02%、0.04%和0.06%的秋水仙碱处理进行突变诱导。再生介质为MS0介质。结果表明,诱导愈伤组织的最佳植物生长调节剂组合为0.5ppm 2.4-D。秋水仙碱的LC50浓度为0.0275%。然而,这种表型是可逆的。需要额外的继代培养期才能达到正常形态
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引用次数: 1
Productivity of Eugenol from Clove (Syzygium aromaticum L.) Under Different Light and Soil Water Conditions 不同光照和土壤水分条件下丁香Eugenol的生产力
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v44i1.2847
A. Setiawan, S. Ito, Y. Mitsuda, R. Hirata, Kiwamu Yamagishi, Yasa Palaguna Umar, Ichiro Kamei
We examined the effect of watering and shading treatments on eugenol productivity of clove seedlings in order to provide the information of the suitable site conditions for eugenol production. After a six-month experiment growing clove seedlings under twelve treatments (3 shading treatments (0%, 60% and 80%) x 4 watering treatments (1.0, 0.75, 0.5 and 0.25 l/m2/day), the total leaf mass per tree (LM), the eugenol content per unit leaf mass (EL) and the eugenol yield per tree (EY) were measured and compared between treatments. As the results, the low watering rates reduced LM slightly; however, this effect was counteracted by the drastically increased EL under the same conditions, resulting in the highest EY in the least watered treatment (0.25 l/m2/day). Heavy shading consistently reduced LM and EL, resulting in the lowest LY under the 80% shading treatment. The relatively dryer site condition where moderate water stress is likely to occur is more suitable for planting clove trees from the aspect of the for long-term high productivity of eugenol, and that the high tree density which may cause a severe competition and a heavy mutual shading among clove trees should be avoided to maintain long term high productivity.
研究了浇水和遮荫处理对丁香幼苗丁香酚产量的影响,以期为丁香酚生产的适宜立地条件提供信息。在12个处理(3个遮荫处理(0%、60%和80%)× 4个浇水处理(1.0、0.75、0.5和0.25 l/m2/d)下进行6个月的丁香幼苗生长试验,测定并比较了不同处理的单株总叶质量(LM)、单位叶质量丁香酚含量(EL)和单株丁香酚产量(EY)。结果表明,低浇水率对LM的影响较小;然而,在相同条件下,这种影响被急剧增加的EL所抵消,导致最少水分处理(0.25 l/m2/天)的EY最高。重度遮光持续降低LM和EL,导致80%遮光处理下的LY最低。从丁香酚长期高产的角度来看,可能发生中度水分胁迫的相对干燥的立地条件更适合种植丁香树,而为了保持丁香酚的长期高产,应避免高密度的立地条件,以免造成丁香树之间的激烈竞争和严重的相互遮蔽。
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引用次数: 0
Elevation Effect on Acacia mangium Volume Estimation 海拔对芒果Acacia材积估算的影响
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2022-02-01 DOI: 10.17503/agrivita.v44i1.3065
Prem Kumar Selvarajan, S. Gandaseca, Kamziah Abd. Kudus, K. Wicaksono
The objective of this study was to examine the effects of four different elevations on Acacia mangium volume in Bintulu, Sarawak. A total of 60 felled trees of A. mangium were used to develop a volume equation by measuring the volume at four different classes of elevation. The study of stand volume estimation in Malaysian plantations, particularly in Bintulu, is sparse. To get the average cross-sectional area, the volume of each tree in each part of the felled trees was estimated using Smalian’s and Newton’s formulas. Four equations were developed in this study to predict the volume from the diameter and analyze the effect of elevation on tree volume. According to the regression analysis, there was a significant relationship between the volume and diameter for each slope steepness. The Very Slope site had the highest volume with an R² value of 0.948 for the model’s fit, which revealed that 94.8% of the data could be evaded. Volume estimation using the regression model can be used to determine and categorize according to its class of elevation, and there is an effect of elevation on standing volume. A. mangium has better growth performance in a very slope area in terms of tree volume compared to different elevations.
本研究的目的是检验砂拉越民都鲁四种不同海拔对芒果Acacia体积的影响。通过测量四个不同海拔等级的体积,使用总共60棵砍伐的芒果树来建立体积方程。马来西亚人工林,特别是民都鲁人工林的林分体积估算研究很少。为了获得平均横截面积,使用Smalian和Newton公式估计了砍伐树木每一部分中每棵树的体积。本研究建立了四个方程,从直径预测体积,并分析海拔对树木体积的影响。根据回归分析,每个坡度的体积和直径之间存在显著关系。Very Slope场地的体积最大,模型拟合的R²值为0.948,这表明94.8%的数据可以回避。使用回归模型的体积估计可以用于根据海拔等级进行确定和分类,海拔对站立体积有影响。A.就树木体积而言,与不同海拔相比,芒果在非常倾斜的地区具有更好的生长性能。
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引用次数: 0
Back Matter 回到问题
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2021-10-01 DOI: 10.17503/agrivita.v43i3.3611
Agrivita Agrivita
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引用次数: 0
Effectiveness of Using Attractants to Control Hypothenemus hampei in Coffea arabica Crop in the Ecuadorian Amazon 引诱剂对厄瓜多尔亚马逊地区阿拉比卡咖啡作物中汉贝氏小蠹的防治效果
IF 0.8 Q3 Agricultural and Biological Sciences Pub Date : 2021-10-01 DOI: 10.17503/agrivita.v43i3.2680
Julio Muñoz-Rengifo, J. A. Rojas, R. Villamar-Torres, J. Reyes-Pérez, S. M. Jazayeri
The present research was aimed to evaluate the effectiveness of attractants placed in craft traps to reduce the bit population (Hypothenemus hampei). Three different mixtures of attractants and control were used in craft traps (4 treatments, which represent the mixtures frequently used by Amazonian coffee growers), arranged in a completely random blocks design. The variable number adult coffee drill was analyzed, as well as the most economical and effective treatment in the capture of adult coffee bit. The data were processed by means of a variance analysis to determine the differences between treatments, and the Tukey media multiple comparison test, at the statistical significance of p<0.05. The results have confirmed that the use of craft traps is a good alternative for adult coffee bit control. The treatment that allowed a greater capture of the coffee bits was T1 (mixture of 2 liters of boiled water + 200 grams of ground coffee), this was also the treatment that had the lowest cost for the elaboration of the artisan trap ($ 14.30). Finally, T1 turned out to be the most effective treatment for adult coffee drill capture in Amazonian conditions of Ecuador. In their study bit capture, ethyl
本研究旨在评估放置在工艺陷阱中的引诱剂减少比特数量的有效性(Hypothenemus hampei)。引诱剂和对照的三种不同混合物被用于工艺陷阱(4种处理,代表亚马逊咖啡种植者经常使用的混合物),以完全随机的块设计排列。分析了可变数量的成人咖啡钻头,以及捕获成人咖啡钻头中最经济有效的处理方法。通过方差分析和Tukey介质多重比较检验对数据进行处理,以确定处理之间的差异,统计显著性为p<0.05。研究结果证实,使用手工陷阱是控制成人咖啡钻头的一个很好的选择。能够更大程度地捕获咖啡渣的处理方法是T1(2升沸水+200克研磨咖啡的混合物),这也是制作手工陷阱成本最低的处理方法(14.30美元)。最后,T1被证明是厄瓜多尔亚马逊地区捕获成人咖啡钻最有效的处理方法。在他们的研究中
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引用次数: 0
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