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Posterior Open Bite Due to Failure of Maxillary Molar Eruption. 上颌磨牙出牙失败后开咬。
IF 0.5 Q3 Medicine Pub Date : 2016-01-01 DOI: 10.2209/tdcpublication.2016-0800
Toshihiko Yasumura, K. Sueishi
Posterior open bite can cause problems with occlusion. It arises from systemic or local factors such as physical or functional interference, ankylosis, and failure of eruption. Primary failure of eruption (PFE) is a rare condition that is difficult to differentiate from ankylosis and requires complex treatment strategies. Here we report a 12-year-old girl who was referred to our hospital by her dentist for re-evaluation of eruption failure of the left maxillary secondary premolar and first and second molars with congenitally missing maxillary lateral teeth. The maxillary first molar was extracted for a therapeutic diagnosis. The left maxillary secondary premolar and second molar reacted well to subsequent orthodontic treatment. Auto-transplantation of the mandibular premolar to the maxil-lary arch was carried out to achieve optimal overjet, overbite, and occlusion. The active treatment period spanned 4 years and 1 month. Assessment of the patient's medical and dental history, prior trauma, and clinical conditions resulted in a therapeutic diagnosis of PFE. Satisfactory orthodontic treatment results were achieved.
后开咬会引起咬合问题。它是由全身或局部因素引起的,如身体或功能的干扰、强直和出疹失败。原发性出疹失败(PFE)是一种罕见的情况,很难与强直区分,需要复杂的治疗策略。我们在此报告一位12岁的女孩,她的牙医转介到我们医院,重新评估左上颌第二前磨牙和第一、第二磨牙的萌出失败,先天性上颌侧牙缺失。拔除上颌第一磨牙进行治疗性诊断。左上颌第二前磨牙和第二磨牙对后续正畸治疗反应良好。自体移植下颌前磨牙至上颌牙弓,以达到最佳的覆盖、覆盖咬合和咬合。积极治疗期4年1个月。评估患者的医疗和牙科病史,先前的创伤和临床条件导致PFE的治疗性诊断。取得了满意的正畸治疗效果。
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引用次数: 3
Participation of Patient Community Members in Communication Classes for Dental Students at Tokyo Dental College. 病人社群成员在东京牙科学院牙科学生交流课上的参与。
IF 0.5 Q3 Medicine Pub Date : 2016-01-01 DOI: 10.2209/tdcpublication.2015-0037
N. Takahashi, Hitoshi Yamamoto, Satoshi Murakami, S. Hirata, N. Sugihara, Riuji Mochizuki, Toshiyuki Takahashi, Yuko Sato, E. Kawada
In 2009, the Ministry of Education, Culture, Sports, Science and Technology launched its "Program for Promoting University Education and Student Support, Theme A: Program for Promoting University Education Reform". The ministry's stated aims were to 1) enhance student training centered on the needs of the individual patient; 2) improve their ability to make comprehensive diagnosis and treatment plans; and 3) instill high ethical standards and good communication skills. One of the main pillars of this project was to establish an educational organization aimed at encouraging public participation, the "Patient Community". The aim was to have members of this community roleplay patients in the Communication Studies component of the 1st-4th years of dental school. It was hoped that they would be able to respond to the students in a more realistic manner than simulated patients. Here, the number of Patient Community members and number who attended Communication Studies classes in 2010, 2011, 2012, and 2014 were investigated. The results showed that the number registered in the Patient Community increased annually, as did the number participating in Communication Studies classes, which rose in accordance with the number of classes held. No difference was observed in the number of attendees per Communication Studies class by grade (years 1-4). The number of members never attending Community Studies classes increased annually, although some members repeatedly attended. These findings suggest that the members who regularly participate tend to remain the same.
2009年,教育、文化、体育、科技部启动了“促进大学教育和学生支持计划,主题A:促进大学教育改革计划”。卫生部声明的目标是:1)加强以患者个体需求为中心的学生培训;2)提高综合诊疗方案制定能力;3)灌输高尚的道德标准和良好的沟通技巧。该项目的主要支柱之一是建立一个旨在鼓励公众参与的教育组织,即“病人社区”。目的是让这个社区的成员在牙科学校一到四年级的传播研究部分扮演病人。希望他们能够以比模拟病人更真实的方式对学生做出反应。本研究调查了2010年、2011年、2012年和2014年患者社区成员人数和参加传播学课程的人数。结果显示,在病人社区注册的人数每年都在增加,参加传播学课程的人数也在增加,这与课程的数量相一致。按年级(1-4年级),每个传播学班的参加人数没有差异。从不参加社区研究课程的成员人数每年都在增加,尽管有些成员经常参加。这些发现表明,经常参加活动的成员倾向于保持不变。
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引用次数: 0
Regional Anatomical Observation of Morphology of Greater Palatine Canal and Surrounding Structures. 大腭管及其周围结构的区域解剖观察。
IF 0.5 Q3 Medicine Pub Date : 2016-01-01 DOI: 10.2209/tdcpublication.2016-1100
Masashi Suzuki, Yuya Omine, Yoshiaki Shimoo, Masahito Yamamoto, A. Kaketa, M. Kasahara, Masamitu Serikawa, S. Rhee, Tadatoshi Matsubayashi, S. Matsunaga, S. Abe
In maxillary molar region implant therapy, support is sometimes obtained from trabecular bone comprising the maxillary tuberosity, pterygoid process of the sphenoid bone, and pyramidal process of the palatine bone. Great care is necessary in such cases due to the presence of the greater palatine canal, which forms a passageway for the greater palatine artery, vein, and nerve. However, clinical anatomical reports envisioning embedding of pterygomaxillary implants in this trabecular bone region have been limited in number. In this study, the 3-D morphology of the greater palatine canal region, including the maxillary tuberosity region and points requiring particular care in pterygomaxillary implantation, were therefore investigated. Micro-CT was used to image 20 dentulous jaws (40 sides) harvested from the dry skulls of Japanese individuals with a mean age of 28.2 years at time of death. The skulls were obtained from the Jikei University School of Medicine cadaver repository. Three-dimensional reconstruction of the trabecular bone region, including the greater palatine canal, was performed using software for 3-D measurement of trabecular bone structure. Trabecular bone region morphometry was performed with the hamular notch-incisive papilla (HIP) plane as the reference plane. The results showed a truncated-cone structure with the greater palatine foramen as the base extending to the pterygopalatine fossa. This indicates the need for care with respect to proximity of the dental implant body to the greater palatine canal and the risk of perforation if it is embedded in the maxillary tuberosity region at an inclination of 60° toward the lingual side. Moreover, caution must be exercised to avoid possible damage to the medial wall of the maxillary sinus if the inclination of the embedded dental implant body is almost perpendicular to the HIP plane.
在上颌磨牙区种植治疗中,有时由上颌粗隆、蝶骨的翼状突和腭骨的锥体突组成的小梁骨提供支持。在这种情况下,由于存在腭大管,形成腭大动脉,静脉和神经的通道,因此需要非常小心。然而,临床解剖报告设想在这个小梁骨区域嵌入翼颌植入物的数量有限。因此,在本研究中,我们研究了腭大管区域的三维形态,包括上颌结节区和翼状颌种植中需要特别注意的点。使用Micro-CT对20个无牙颌(40侧)进行成像,这些颌骨取材于日本人的干颅骨,死亡时平均年龄为28.2岁。这些头骨来自智庆大学医学院的尸体库。使用小梁骨结构三维测量软件对包括腭大管在内的小梁骨区域进行三维重建。小梁骨区形态测量以髋部凹痕平面为参考平面。结果显示为截断锥状结构,以腭大孔为基部延伸至翼腭窝。这表明需要注意种植体靠近腭大管的位置,如果种植体嵌入上颌结节区并向舌侧倾斜60°,则有穿孔的风险。此外,如果嵌入种植体的倾斜度几乎垂直于髋关节平面,则必须小心避免可能对上颌窦内侧壁造成损伤。
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引用次数: 6
Tooth Loss in Problem-oriented, Irregular, and Regular Attenders at Dental Offices. 牙齿脱落的问题导向,不定期和定期到牙科诊所就诊。
IF 0.5 Q3 Medicine Pub Date : 2016-01-01 DOI: 10.2209/tdcpublication.57.11
K. Yoshino, Koji Ito, M. Kuroda, N. Sugihara
The aim of this retrospective study was to compare number of teeth lost among regular attenders (RAs), irregular attenders (IRAs), and problem-oriented attenders (POAs) at dental offices over a 10-yr observation period. Information on tooth loss was obtained from general practitioners. Patients were divided into 3 groups based on appointment adherence. A total of 1,886 teeth were lost in 1,400 patients. The mean number of teeth lost in men was 2.2±2.6 per patient over 10 yr in POAs, 1.2±1.7 in IRAs, and 1.5±1.5 in RAs. This number was significantly lower in IRAs (p = 0.011) or RAs (p=0.012) than in POAs. When the dependent variable was defined as "a patient with at least 2 or more extracted teeth", the independent variables showed the following correlations with tooth loss: IRAs (OR: 0.54; 95%CI: 0.35-0.84), RAs (OR: 0.65; 95%CI: 0.47-0.89), man (OR: 1.43; 95%CI: 1.11-1.83), hypertension (OR: 1.38; 95%CI: 1.04-1.85), 20-25 present teeth (OR: 2.41; 95%CI: 1.81-3.22), and 1-19 present teeth (OR: 3.75; 95%CI: 2.73-5.16). The risk of tooth loss showed a 0.65-fold increase in RAs undergoing maintenance compared with POAs. Motivating patients to visit the dentist more regularly and undergo maintenance is important. The present results may be of use to dental professionals in providing patients with detailed information on potential tooth loss and associated risk factors with the aim of achieving such a change in behavior.
本回顾性研究的目的是在10年的观察期内比较牙科诊所定期就诊(RAs)、不定期就诊(ira)和问题导向就诊(POAs)的牙齿脱落数量。有关牙齿脱落的信息来自全科医生。根据预约依从性将患者分为3组。1400名患者共脱落1886颗牙齿。男性10年内平均脱落牙数poa为2.2±2.6颗,ira为1.2±1.7颗,RAs为1.5±1.5颗。IRAs (p= 0.011)或RAs (p=0.012)的这一数字明显低于poa。当因变量定义为“至少拔了2颗以上牙齿的患者”时,自变量与牙齿脱落的相关性如下:IRAs (or: 0.54;95%CI: 0.35-0.84), RAs (OR: 0.65;95%CI: 0.47-0.89),男性(OR: 1.43;95%CI: 1.11-1.83),高血压(OR: 1.38;95%CI: 1.04-1.85), 20-25颗现牙(OR: 2.41;95%CI: 1.81-3.22), 1-19颗现牙(OR: 3.75;95%置信区间:2.73—-5.16)。与poa相比,接受维护的ra患者牙齿脱落的风险增加了0.65倍。激励病人更经常地去看牙医并接受保养是很重要的。目前的结果可能对牙科专业人员有用,为患者提供有关潜在牙齿脱落和相关风险因素的详细信息,目的是实现这种行为的改变。
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引用次数: 19
Effect of Cuff Pressure Elevation on Internal Diameter of Tracheal Tube in Simulated Trachea. 模拟气管中袖套压力升高对气管内径的影响。
IF 0.5 Q3 Medicine Pub Date : 2016-01-01 DOI: 10.2209/tdcpublication.57.7
Yukiko Matsuki, N. Matsuura, T. Ichinohe
Application of nitrous oxide during anesthesia causes an increase in tracheal tube cuff pressure over time. The purpose of this study was to investigate the effect of an increase in cuff pressure on 3 types of tube (the Portex, Mallinckrodt, and Parker) commonly used for nasotracheal intubation. A cylindrical vessel was used to simulate a trachea. Cuff pressure was set at 0 cmH2O (R0) or 20 cmH2O (R20) at room temperature, or at 20 cmH2O (H20), 40 cmH2O (H40), 60 cmH2O (H60), or 80 cmH2O (H80) in 38°C hot water and pressure applied for 30 min. The value obtained at R0 was used as a reference (100%) and the rate of change under each condition determined. No change was observed at R20 in any of the 3 groups. In 38°C hot water, internal diameter in the Portex group decreased by 5.4% at H20 and 7.3% at H40, while that in the Mallinckrodt group decreased by 6% at H40. No significant change was observed in internal diameter in the Parker group, even when cuff pressure was increased. The internal diameter in the Portex group was the smallest at all cuff pressures in hot water. When the nasotracheal intubation tubes selected were placed in a simulated trachea and cuff pressure increased, internal diameter in the Portex and Mallinckrodt groups decreased.
在麻醉过程中使用一氧化二氮会随着时间的推移导致气管套管压力的增加。本研究的目的是探讨袖带压力增加对鼻气管插管常用的3种导管(Portex、Mallinckrodt和Parker)的影响。一个圆柱形容器被用来模拟气管。在室温下将袖带压力设置为0 cmH2O (R0)或20 cmH2O (R20),或在38°C热水中设置为20 cmH2O (H20)、40 cmH2O (H40)、60 cmH2O (H60)或80 cmH2O (H80),并施加压力30分钟。在R0处获得的值作为参考(100%),并确定每种条件下的变化率。在R20时,三组均未见变化。在38°C热水中,Portex组的内径在H20和H40下分别下降了5.4%和7.3%,而Mallinckrodt组的内径在H40下下降了6%。即使袖带压力增加,Parker组的内径也没有明显变化。在热水中所有袖带压力下,Portex组的内径最小。将选择的鼻气管插管置于模拟气管中,当袖带压力增加时,Portex组和Mallinckrodt组的内径减小。
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引用次数: 1
Radiopacity Measurement of Restorative Resins Using Film and Three Digital Systems for Comparison with ISO 4049: International Standard. 用胶片和三数字系统测量恢复树脂的不透明度与ISO 4049:国际标准的比较。
IF 0.5 Q3 Medicine Pub Date : 2015-01-01 DOI: 10.2209/tdcpublication.56.207
R. Kapila, Y. Matsuda, K. Araki, T. Okano, K. Nishikawa, T. Sano
This study compared Ultra Speed Occlusal Film (USOF) and 3 digital systems in determining the radiopacity of 5 different restorative resins in terms of equivalents of aluminum thickness. Whether those digital systems could be used to determine whether radiopacity was in line with International Organization for Standardization (ISO) recommendations was also investigated. Disks of each of 5 restorative resins and an aluminum step wedge were exposed at 65 kVp and 10 mA on USOF and imaged with each digital system. Optical density on the film was measured with a transmission densitometer and the gray values on the digital images using Image J software. Graphs showing gray value/optical density to step wedge thickness were constructed. The aluminum equivalent was then calculated for all the resins using a regression equation. All the resins were more radiopaque than 1 mm of aluminum, and therefore met the ISO 4049 recommendations for restorative resins. Some resins showed statistically higher aluminum equivalents with digital imaging. The use of traditional X-ray films is declining, and digital systems offer many advantages, including an easy, fast, and reliable means of determining the radiopacity of dental materials.
本研究比较了超高速咬合膜(USOF)和3种数字系统在确定5种不同的修复树脂的放射不透明度方面的铝厚度的等效。还调查了这些数字系统是否可用于确定放射不透明度是否符合国际标准化组织(ISO)的建议。在65 kVp和10 mA的USOF下暴露5种修复树脂的磁盘和铝台阶楔,并在每个数字系统上成像。用透射密度计测量胶片上的光密度,用Image J软件对数字图像进行灰度值处理。构建了灰度值/光密度与阶跃楔形厚度的关系图。然后使用回归方程计算所有树脂的铝当量。所有树脂都比1毫米的铝更不透光,因此符合ISO 4049关于修复树脂的建议。一些树脂在数字成像中显示出更高的铝当量。传统x光片的使用正在减少,而数字系统提供了许多优点,包括一种简单、快速和可靠的方法来确定牙科材料的放射性不透明度。
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引用次数: 13
Metabolic Syndrome, Lifestyle, and Dental Caries in Japanese School Children. 日本学龄儿童的代谢综合征、生活方式和龋齿。
IF 0.5 Q3 Medicine Pub Date : 2015-01-01 DOI: 10.2209/tdcpublication.56.233
H. Osawa, N. Sugihara, T. Ukiya, Y. Ishizuka, D. Birkhed, Masaru Hasegawa, T. Matsukubo
The number of children with Metabolic Syndrome (MetS) has recently been increasing in Japan. Few studies have investigated the relationship between MetS and oral health. The aim of this study was to investigate the association between MetS, lifestyle, and oral health status in school children. Our goal is to utilize these results in health education aimed at preventing the onset of MetS in school children and adults. A total of 689 Japanese children (365 boys and 324 girls) aged between 10 and 13 years were examined and waist circumference (WC), ratio of WC to height, blood pressure, fasting blood sugar (FBS), high density lipoprotein-cholesterol, and triglyceride values determined together with oral health status, including dental caries experience (DMFT). The results revealed that 6.5% of the children fell under the health board recognized "MetS or high risk of MetS" (MetS/HR) classification. A total of 140 (20%) children had a high Streptococcus mutans count. The mean WC, FBS, and DMFT values were significantly greater in children with a high salivary S. mutans count (p<0.05). Multiple logistic regression analysis revealed a statistically significance association between MetS/HR, non-breakfast eaters (odds ratio (OR): 2.70), no regular exercise (OR: 2.60), and a high salivary S. mutans count (≥10(5) CFU/ml; OR: 2.18; p<0.05). The present results indicate that lifestyle and salivary S. mutans count could be useful in screening children for MetS/HR. These variables may be useful in targeting interventions aimed at preventing MetS in school children.
最近,日本患有代谢综合征(MetS)的儿童人数不断增加。很少有研究调查MetS与口腔健康之间的关系。本研究旨在探讨学龄儿童的met、生活方式和口腔健康状况之间的关系。我们的目标是利用这些结果进行健康教育,旨在预防学龄儿童和成人发生MetS。对689名年龄在10至13岁之间的日本儿童(365名男孩和324名女孩)进行了检查,并测定了腰围(WC)、腰围与身高之比、血压、空腹血糖(FBS)、高密度脂蛋白-胆固醇和甘油三酯值,以及口腔健康状况,包括龋齿经历(DMFT)。结果显示,6.5%的儿童属于卫生委员会认可的“MetS或MetS高风险”(MetS/HR)分类。共有140例(20%)患儿的变形链球菌计数较高。唾液变形链球菌计数高的儿童WC、FBS和DMFT平均值显著高于对照组(p<0.05)。多元logistic回归分析显示MetS/HR、不吃早餐者(比值比(OR): 2.70)、不经常运动(OR: 2.60)和高唾液变形链球菌计数(≥10(5)CFU/ml;OR: 2.18;p < 0.05)。目前的结果表明,生活方式和唾液突变链球菌计数可能有助于筛查儿童是否患有MetS/HR。这些变量可能有助于针对预防学龄儿童met的干预措施。
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引用次数: 10
Case of Severe Maxillary Protrusion Accompanied by Crowding and Scissor Bite. 上颌严重突出伴拥挤、剪咬1例。
IF 0.5 Q3 Medicine Pub Date : 2015-01-01 DOI: 10.2209/tdcpublication.56.243
Hidenori Katada, K. Sueishi
This case involved a 30-year-old woman who visited our hospital with the main complaint of protrusion of the maxillary incisors and upper and lower lips. She had difficulty closing her lips, and a chin button was observed when the lips were closed. The skeletal pattern showed maxillary protrusion and mandibular retrusion, and the mandible showed severe high angle. Labial inclination of both the maxillary and mandibular incisors was found, as well as crowding. In addition, the maxillary left second molar showed buccal displacement, and scissor bite was evident in the left second molar region. The bilateral molar relationship was cusp-to-cusp class II malocclusion. Angle class II maxillary protrusion accompanied by crowding and left second molar scissor bite was diagnosed. Surgical orthodontic treatment was judged as the best approach to treat the jaw deformities. However, in line with the wishes of the patient, treatment was undertaken using implant anchors instead. Straight-wire brackets with a 0.022-inch slot were fitted. A lingual arch was placed in the mandible and plate-type implant anchors in the first molar region of the maxilla. Almost no change was observed in skeletal pattern as no surgery was performed. The maxillary incisors moved back 10 mm, however, and the mandibular incisors showed an improvement of 4 mm from L1 to APo. The upper and lower lips consequently moved back 7 mm with respect to the E-line. Active treatment required 3 years and 6 months. Esthetic and functional improvements were achieved.
本病例涉及一名30岁妇女,她以上颌门牙和上下唇突出为主要主诉来我院就诊。她很难合上嘴唇,当嘴唇闭上时,可以看到下巴上有一个钮扣。骨型表现为上颌前突、下颌骨后缩,下颌骨呈严重的高角。上颌和下颌切牙均有唇倾,并有拥挤现象。上颌左侧第二磨牙出现颊部移位,左侧第二磨牙区出现明显的剪咬。双侧磨牙关系为尖对尖II类错颌。角II型上颌突出伴拥挤,左第二磨牙剪刀咬合。外科正畸治疗是治疗颌骨畸形的最佳方法。然而,根据患者的意愿,我们改用种植体锚钉进行治疗。安装带有0.022英寸槽的直丝托架。在下颌骨放置舌弓,在上颌骨第一磨牙区放置板型种植锚。由于没有进行手术,骨骼形态几乎没有变化。上颌切牙由L1向APo后移10mm,下颌切牙由L1向APo后移4mm。因此,上下嘴唇相对于e线向后移动了7毫米。积极治疗需3年零6个月。实现了美学和功能的改进。
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引用次数: 1
Basic Finite Element Analysis of Para-periodontal Ligament in All-ceramic Zirconia Fixed Partial Denture. 全陶瓷氧化锆固定局部义齿牙周旁韧带的基本有限元分析。
IF 0.5 Q3 Medicine Pub Date : 2015-01-01 DOI: 10.2209/tdcpublication.56.215
Syuntaro Nomoto, S. Matsunaga, Toru Sato, Mamoru Yotsuya, S. Abe
The purpose of the present study was to investigate the validity of incorporating a para-periodontal ligament in the test mold used in a basic fracture test of a zirconia all-ceramic fixed partial denture (FPD). A simplified three-dimensional finite element analysis model was designed based on the three-unit FPD fracture test. Two types of model, one with and one without a para-periodontal ligament between the abutment and base mold, were fabricated. Microfocus CT of the missing first molar area in a dry human mandible was performed. A three-dimensional model was then fabricated based on the data obtained. A load of 600 N was applied to the center of the pontic and stress distribution observed. The model with the para-periodontal ligament showed stress dispersion to the dental root with rotation of the abutment mold. Stress distribution in the finite element analysis model with a para-periodontal ligament showed greater similarity with that in the mandibular model than with that in the other two models without a para-periodontal ligament.
本研究的目的是探讨在氧化锆全陶瓷固定局部义齿(FPD)基本断裂试验中使用的测试模具中加入牙周旁韧带的有效性。基于三单元FPD断裂试验,设计了简化的三维有限元分析模型。制作两种模型,一种是在基台和基模之间有和没有牙周旁韧带。对干燥人下颌骨缺失的第一磨牙区域进行微聚焦CT扫描。然后根据所获得的数据制作三维模型。在桥体中心施加600 N的荷载,观察到应力分布。带牙周旁韧带的模型随着基模的旋转,应力向牙根分散。有牙周旁韧带有限元分析模型的应力分布与下颌模型的相似度高于无牙周旁韧带有限元分析模型的应力分布。
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引用次数: 3
Transient Receptor Potential Cation Channel Subfamily Vanilloid Member 3 is not Involved in Plasma Membrane Stretch-induced Intracellular Calcium Signaling in Merkel Cells. 瞬时受体电位阳离子通道亚家族香草酸蛋白3成员不参与默克尔细胞质膜拉伸诱导的细胞内钙信号传导。
IF 0.5 Q3 Medicine Pub Date : 2015-01-01 DOI: 10.2209/tdcpublication.56.259
A. Higashikawa, Y. Kojima, Masaki Sato, M. Kimura, Kazuhiro Ogura, H. Mochizuki, T. Sase, Akinori Shinya, Kunio Kobune, Tadashi Furuya, Toru Sato, Y. Shibukawa, M. Tazaki
Merkel cells (MCs), which form part of the MC-neurite complex, making contact with sensory afferents to drive mechanosensory transduction mechanisms, express transient receptor potential (TRP) cation channel subfamily vanilloid (V) members 1, 2, and 4, as well as ankyrin subfamily member 1. While these proteins are involved in sensing plasma membrane stretch, less is known about the functional properties of TRPV subfamily member 3 (TRPV3) during membrane stretch in MCs. The aim of this study was to determine whether TRPV3 channels were involved in mechanosensory activity by measuring intracellular free Ca(2+) concentrations ([Ca(2+)]i) in MCs isolated from hamster buccal mucosa. Application of a hypotonic extracellular solution to quinacrine-positive MCs elicited a transient increase in [Ca(2+)]i. When TRPV3 channel antagonist 2,2-diphenyltetrahydrofuran was added to the hypotonic extracellular solution, however, no effect was observed on hypotonic stimulation-induced increase in [Ca(2+)]i. These results suggest that TRPV3 channels are not involved in the mechanosensory mechanism during membrane stretch in MCs.
默克尔细胞(MCs)是mc -神经突复合体的一部分,通过与感觉传入接触来驱动机械感觉转导机制,表达瞬时受体电位(TRP)阳离子通道亚家族香兰素(V)成员1、2和4,以及锚蛋白亚家族成员1。虽然这些蛋白参与感知质膜拉伸,但对TRPV亚家族成员3 (TRPV3)在MCs膜拉伸中的功能特性知之甚少。本研究的目的是通过测量从仓鼠口腔粘膜分离的MCs细胞内游离Ca(2+)浓度([Ca(2+)]i)来确定TRPV3通道是否参与机械感觉活动。将低渗细胞外溶液应用于醌阳性MCs可引起[Ca(2+)]i的短暂增加。然而,当TRPV3通道拮抗剂2,2-二苯四氢呋喃加入低渗细胞外溶液时,对低渗刺激诱导的[Ca(2+)]i的增加没有影响。这些结果表明,TRPV3通道不参与MCs膜拉伸过程中的机械感觉机制。
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引用次数: 2
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Bulletin of Tokyo Dental College
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