Bianca M. Marcella, Mia S. Geromella, Ahmad Mohammad, Jake Sweezey-Munroe, R. MacPherson, Val A. Fajardo
The sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) actively transports Ca2+ into the sarcoplasmic reticulum to facilitate cardiac muscle relaxation. Phospholamban (PLN) allosterically inhibits SERCA, and an imbalance of SERCA2a, dominant cardiac isoform, and PLN content disrupts Ca2+ homeostasis and cardiac contractility. A previous study has shown that ovariectomized (OVX) rats have reduced SERCA activity due to lowered SERCA2a and increased PLN content. Furthermore, it was found that forced treadmill running in OVX rats restored SERCA activity and protein content levels. Here, we investigated whether voluntary wheel running (VWR) would produce similar effects on cardiac SERCA function in OVX mice. Female mice were divided into the following groups for 8 weeks: SHAM; OVX; SHAM + VWR; and OVX + VWR (n = 10/group). SERCA activity and Ca2+ uptake assays were performed in cardiac muscle homogenates. Protein levels of SERCA2, PLN, and pPLN were determined via Western blot analysis. We found statistical interactions for Ca2+ uptake, maximal SERCA activity, and SERCA2a content where VWR increased these parameters in SHAM mice but not in OVX mice. We detected a main effect of OVX on PLN content, and main effects of OVX and VWR on pPLN content. The OVX mice ran significantly less than the SHAM mice, suggesting that estrogen deprivation and lack of regular exercise may blunt the effects of voluntary aerobic exercise on cardiac SERCA function.
{"title":"Characterizing the Effects of Voluntary Wheel Running on Cardiac SERCA Function in Ovariectomized Mice","authors":"Bianca M. Marcella, Mia S. Geromella, Ahmad Mohammad, Jake Sweezey-Munroe, R. MacPherson, Val A. Fajardo","doi":"10.3390/muscles1030016","DOIUrl":"https://doi.org/10.3390/muscles1030016","url":null,"abstract":"The sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) actively transports Ca2+ into the sarcoplasmic reticulum to facilitate cardiac muscle relaxation. Phospholamban (PLN) allosterically inhibits SERCA, and an imbalance of SERCA2a, dominant cardiac isoform, and PLN content disrupts Ca2+ homeostasis and cardiac contractility. A previous study has shown that ovariectomized (OVX) rats have reduced SERCA activity due to lowered SERCA2a and increased PLN content. Furthermore, it was found that forced treadmill running in OVX rats restored SERCA activity and protein content levels. Here, we investigated whether voluntary wheel running (VWR) would produce similar effects on cardiac SERCA function in OVX mice. Female mice were divided into the following groups for 8 weeks: SHAM; OVX; SHAM + VWR; and OVX + VWR (n = 10/group). SERCA activity and Ca2+ uptake assays were performed in cardiac muscle homogenates. Protein levels of SERCA2, PLN, and pPLN were determined via Western blot analysis. We found statistical interactions for Ca2+ uptake, maximal SERCA activity, and SERCA2a content where VWR increased these parameters in SHAM mice but not in OVX mice. We detected a main effect of OVX on PLN content, and main effects of OVX and VWR on pPLN content. The OVX mice ran significantly less than the SHAM mice, suggesting that estrogen deprivation and lack of regular exercise may blunt the effects of voluntary aerobic exercise on cardiac SERCA function.","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90697261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.02
D. Mazza, A. Annibaldi, E. Monaco, A. Carrozzo, G. Princi, S. Fenucci, E. Viglietta, S. Porcino, G. Rossi, A. Ferretti
{"title":"Achilles Tendon Rupture in Professional Football Player: an Epidemiological Study in European Championship With A Mid-Term Follow-Up","authors":"D. Mazza, A. Annibaldi, E. Monaco, A. Carrozzo, G. Princi, S. Fenucci, E. Viglietta, S. Porcino, G. Rossi, A. Ferretti","doi":"10.32098/mltj.04.2022.02","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.02","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90545605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.14
P. Kumar, S. Varshney, N. Girish
{"title":"Relation Between Components of Oral Health and Sarcopenia in Older Adults: a Narrative Review","authors":"P. Kumar, S. Varshney, N. Girish","doi":"10.32098/mltj.04.2022.14","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.14","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84520598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.04
I. Dimitrov, T. Stankova, P. Angelova, N. Boyadjiev, K. Georgieva, D.I. Popov, I. Dimov, A. Bivolarska, F. Gerginska, E. Daskalova, M. Draganova-Filipova, S. Delchev
{"title":"Inflammatory Response of Skeletal Muscles to a High-Fat Diet","authors":"I. Dimitrov, T. Stankova, P. Angelova, N. Boyadjiev, K. Georgieva, D.I. Popov, I. Dimov, A. Bivolarska, F. Gerginska, E. Daskalova, M. Draganova-Filipova, S. Delchev","doi":"10.32098/mltj.04.2022.04","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.04","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75634959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.18
M. Abdelkreem, K. Hussein, A. Moshrif
{"title":"Ultrasound-Guided Injection of Low Molecular Weight Hyaluronic Acid versus Steroid for Inflammatory and Degenerative Sacroiliitis: a Single-Blinded Randomized Controlled Trial","authors":"M. Abdelkreem, K. Hussein, A. Moshrif","doi":"10.32098/mltj.04.2022.18","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.18","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88647776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.10
G. Krist, B. Koc, R. Borghans, M. Schotanus, E. Jansen
{"title":"Comparable Tibial Tunnel Widening despite Compound Differences of Bioabsorbable Interference Screws in Anterior Cruciate Ligament Reconstruction: a Retrospective Study","authors":"G. Krist, B. Koc, R. Borghans, M. Schotanus, E. Jansen","doi":"10.32098/mltj.04.2022.10","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.10","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87430242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.01
E. Roh, M. S. Rana, K. McInnis, D. Bakal, H. Borgstrom, J. Borg-Stein
{"title":"Orthobiologic Injectables in the Ankle Joint: a Narrative Review","authors":"E. Roh, M. S. Rana, K. McInnis, D. Bakal, H. Borgstrom, J. Borg-Stein","doi":"10.32098/mltj.04.2022.01","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.01","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80324903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.06
S. I. Alaqaili, M. Al-Saati, M. Y. AlYousef, M. AlZayer, A. Alsultan, M. Alzahrani, S. Alqahtani
{"title":"Efficacy of Liposomal Bupivacaine in Arthroscopic Rotator Cuff Repair: a Systematic Review and Meta-Analysis of Randomized Controlled Trials","authors":"S. I. Alaqaili, M. Al-Saati, M. Y. AlYousef, M. AlZayer, A. Alsultan, M. Alzahrani, S. Alqahtani","doi":"10.32098/mltj.04.2022.06","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.06","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74869130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.13
A. Shamlou Kazemi, F. Babakhani, R. Sheikhhoseini, H. Naderifar
{"title":"Effect of Eight-Week Proprioceptive Neuromuscular Facilitation (PNF) Pattern-Based Exercises on Performance Scores and Postural Stability in Elite Men Basketball Players","authors":"A. Shamlou Kazemi, F. Babakhani, R. Sheikhhoseini, H. Naderifar","doi":"10.32098/mltj.04.2022.13","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.13","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89752895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.32098/mltj.04.2022.19
P. Puagprakong, A. Kanjanasilanont, K. Sornkaew, W.S. Brady
{"title":"The Effects of Lower Crossed Syndrome on Upper Body Posture during Sitting in Female Office Workers","authors":"P. Puagprakong, A. Kanjanasilanont, K. Sornkaew, W.S. Brady","doi":"10.32098/mltj.04.2022.19","DOIUrl":"https://doi.org/10.32098/mltj.04.2022.19","url":null,"abstract":"","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87331781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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