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Antioxidant, antiglycation, and antibacterial of copper oxide nanoparticles synthesized using Caesalpinia Sappan extract. 利用蓝花楹提取物合成的氧化铜纳米粒子的抗氧化、抗糖化和抗菌作用
IF 1.9 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-09 Epub Date: 2024-06-28 DOI: 10.5582/ddt.2024.01030
Mathurada Sasarom, Phenphichar Wanachantararak, Pisaisit Chaijareenont, Siriporn Okonogi

Synthesis of metal nanoparticles using plant extracts is environmentally friendly and of increasing interest. However, not all plant extracts can meet successfully on the synthesis. Therefore, searching for the high potential extracts that can reduce the metal salt precursor in the synthesis reaction is essential. The present study explores the synthesis of copper oxide nanoparticles (CuONPs) using Caesalpinia sappan heartwood extract. Phytochemical analysis and determination of the total phenolic content of the extract were performed before use as a reducing agent. Under the suitable synthesized condition, a color change in the color of the solutions to brown confirmed the formation of CuONPs. The obtained CuONPs were confirmed using ultraviolet-visible spectroscopy, photon correlation spectroscopy, X-ray diffraction, scanning electron microscope, energy dispersive X-ray, and Fourier transform infrared analysis. The synthesized CuONPs investigated for antioxidant, antiglycation, and antibacterial activities. CuONPs possessed antioxidant activities by quenching free radicals with an IC50 value of 63.35 µg/mL and reducing activity with an EC range of 3.19-10.27 mM/mg. CuONPs also inhibited the formation of advanced glycation end products in the bovine serum albumin/ribose model with an IC50 value of 17.05 µg/mL. In addition, CuONPs showed inhibition of human pathogens, including Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, and prevention of biofilm formation and biofilm eradication, with maximum inhibition of approx. 75%. Our findings suggest that C. sappan extract can be used to obtain highly bioactive CuONPs for the development of certain medical devices and therapeutic agents.

利用植物提取物合成金属纳米粒子是一种环保的方法,越来越受到人们的关注。然而,并非所有植物提取物都能成功用于合成。因此,寻找能在合成反应中减少金属盐前体的高潜力提取物至关重要。本研究探讨了使用红豆杉心材提取物合成氧化铜纳米颗粒(CuONPs)。在用作还原剂之前,对提取物进行了植物化学分析和总酚含量测定。在合适的合成条件下,溶液颜色变为棕色证实了 CuONPs 的形成。利用紫外-可见光谱、光子相关光谱、X 射线衍射、扫描电子显微镜、能量色散 X 射线和傅立叶变换红外分析对所获得的 CuONPs 进行了确认。对合成的 CuONPs 进行了抗氧化、抗糖化和抗菌活性研究。CuONPs 具有淬灭自由基的抗氧化活性,IC50 值为 63.35 µg/mL,还具有还原活性,EC 值范围为 3.19-10.27 mM/mg。CuONPs 还能在牛血清白蛋白/核糖模型中抑制高级糖化终产物的形成,IC50 值为 17.05 µg/mL。此外,CuONPs 还具有抑制人类病原体(包括革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌)、防止生物膜形成和消除生物膜的作用,最大抑制率约为 75%。我们的研究结果表明,C. sappan 提取物可用于获得高生物活性的 CuONPs,以开发某些医疗器械和治疗药物。
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引用次数: 0
Induction of acute silkworm hemolymph melanization by Staphylococcus aureus treated with peptidoglycan-degrading enzymes. 用肽聚糖降解酶处理的金黄色葡萄球菌诱导急性家蚕血淋巴黑化。
IF 1.9 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-09 Epub Date: 2024-06-26 DOI: 10.5582/ddt.2024.01026
Yasuhiko Matsumoto, Eri Sato, Takashi Sugita

Staphylococcus aureus, a Gram-positive bacterium, causes inflammatory skin diseases, such as atopic dermatitis, and serious systemic diseases, such as sepsis. In the skin and nasal environment, peptidoglycan (PGN)-degrading enzymes, including lysozyme and lysostaphin, affects S. aureus PGN. However, the effects of PGN-degrading enzymes on the acute innate immune-inducing activity of S. aureus have not yet been investigated. In this study, we demonstrated that PGN-degrading enzymes induce acute silkworm hemolymph melanization by S. aureus. Insoluble fractions of S. aureus treated with lysozyme, lysostaphin, or both enzymes, were prepared. Melanization of the silkworm hemolymph caused by the injection of these insoluble fractions was higher than that of S. aureus without enzyme treatment. These results suggest that structural changes in S. aureus PGN caused by PGN-degrading enzymes affect the acute innate immune response in silkworms.

金黄色葡萄球菌是一种革兰氏阳性细菌,可引起炎症性皮肤病(如特应性皮炎)和严重的全身性疾病(如败血症)。在皮肤和鼻腔环境中,肽聚糖(PGN)降解酶(包括溶菌酶和溶菌酶)会影响金黄色葡萄球菌的 PGN。然而,PGN 降解酶对金黄色葡萄球菌急性先天性免疫诱导活性的影响尚未得到研究。在这项研究中,我们证明了 PGN 降解酶能诱导金黄色葡萄球菌使家蚕急性血淋巴黑色化。我们制备了用溶菌酶、溶血素或两种酶处理的金黄色葡萄球菌的不溶性部分。注入这些不溶性部分后,家蚕血淋巴的黑色化程度高于未经酶处理的金黄色葡萄球菌。这些结果表明,PGN降解酶引起的金黄色葡萄球菌PGN结构变化会影响家蚕的急性先天性免疫反应。
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引用次数: 0
Shiikuwasha leaf and peel extracts inhibit allergic reactions by suppressing degranulation in RBL-2H3 rat basophilic leukemia cells and immunoglobulin production in mouse spleen lymphocytes. 水曲柳叶和皮提取物通过抑制 RBL-2H3 大鼠嗜碱性白血病细胞的脱颗粒作用和小鼠脾脏淋巴细胞的免疫球蛋白生成,从而抑制过敏反应。
IF 1.9 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-09 Epub Date: 2024-06-06 DOI: 10.5582/ddt.2024.01016
Takeaki Okamoto, Sayaka Yokoyama, Hinako Ushimaru, Mamoru Tanaka

This study aims to investigate the antiallergic effects of Shiikuwasha (Citrus depressa Hayata) leaf and peel extracts by examining the regulation of degranulation and inflammatory cytokine production from rat basophilic leukemia (RBL-2H3) cells and antigen-specific antibody production in sensitized mouse spleen lymphocytes. In vivo antiallergic activity was evaluated using the passive cutaneous anaphylaxis (PCA) reaction model. Extracts of Shiikuwasha leaves and peel were prepared using 80% methanol and dissolved in dimethylsulfoxide. The dinitrophenyl-human serum albumin-induced β-hexosaminidase levels in immunoglobulin (Ig) E-sensitized RBL-2H3 cells were assessed using enzymatic assays. Cytokine production was measured by enzyme-linked immunosorbent assay. Antibody production capacity was evaluated using lymphocytes isolated from spleens of type I allergy model mice. Lymphocytes were cultured for 72 h with Shiikuwasha extracts, and ovalbumin-specific IgE, IgG1, and IgG2a levels were measured. Shiikuwasha leaf and peel extract significantly reduced β-hexosaminidase release and suppressed interleukin-4 and tumor necrosis factor-α production from RBL-2H3 cells. Ovalbumin-specific IgE and IgG1 production decreased in Shiikuwasha extract-treated lymphocytes. These extracts also significantly suppressed the PCA reaction. Shiikuwasha leaf and peel extract reduce degranulation in RBL-2H3 cells and antibody production in spleen-derived lymphocytes and therefore exhibit antiallergic effects.

本研究旨在通过检测对大鼠嗜碱性粒细胞白血病(RBL-2H3)细胞脱颗粒和炎性细胞因子产生的调节作用,以及对致敏小鼠脾淋巴细胞抗原特异性抗体产生的调节作用,来研究Shiikuwasha(Citrus depressa Hayata)叶片和果皮提取物的抗过敏作用。使用被动皮肤过敏性休克(PCA)反应模型对体内抗过敏活性进行了评估。用 80% 的甲醇制备 Shiikuwasha 叶子和果皮的提取物,并将其溶解在二甲基亚砜中。用酶法检测免疫球蛋白(Ig)E致敏的RBL-2H3细胞中二硝基苯基人血清白蛋白诱导的β-己糖胺酸酶水平。细胞因子的产生是通过酶联免疫吸附试验测定的。使用从 I 型过敏模型小鼠脾脏中分离出的淋巴细胞评估抗体生成能力。淋巴细胞在水曲柳提取物的作用下培养 72 小时,然后测定卵清蛋白特异性 IgE、IgG1 和 IgG2a 水平。水曲柳叶和皮提取物能显著减少β-己糖胺酶的释放,抑制RBL-2H3细胞产生白细胞介素-4和肿瘤坏死因子-α。Shiikuwasha提取物处理过的淋巴细胞产生的卵清蛋白特异性IgE和IgG1减少。这些提取物还能明显抑制 PCA 反应。Shiikuwasha叶和皮提取物可减少RBL-2H3细胞的脱颗粒和脾源性淋巴细胞的抗体产生,因此具有抗过敏作用。
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引用次数: 0
Stability of ischial pressure with 3D thermoplastic elastomer cushion and the characteristics of four types of cushions in pressure redistribution. 三维热塑性弹性体垫对骶骨压力的稳定性以及四种垫子在压力再分布方面的特点。
IF 1.9 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-09 Epub Date: 2024-06-15 DOI: 10.5582/ddt.2024.01025
Yoshiyuki Yoshikawa, Kyoko Nagayoshi, Noriaki Maeshige, Atomu Yamaguchi, Yuki Aoyama, Shuto Takita, Teppei Wada, Masayuki Tanaka, Hiroto Terashi, Yuma Sonoda

Wheelchair cushions are recommended to be used with wheelchair and can protect the buttocks from pain and injury by relieving interface pressure for wheelchair users. However, further investigations are required for proper use in response to the development of new types of wheelchair cushions. The objective of this study was to evaluate physical characteristics of wheelchair cushions by comparing pressure redistributing effects of four types of cushions. The participants were 16 healthy adults who consented to participate in this study. A pressure mapping system (CONFORMat, Nitta Corp.) was used for the measurements. Pressure at ischium was measured immediately after the stabilization of the sitting posture and 10 minutes after. The pressure at ischium significantly decreased with any wheelchair cushions (P < 0.01). A significant negative correlation between body mass index and pressure at ischium was observed without a wheelchair cushion (r = - 0.70), however, the correlation disappeared upon use of a wheelchair cushion. The pressure at ischium increased over time with cushions of urethane, air, and urethane-air hybrid while that with the 3D thermoplastic elastomer cushion did not, and the change in the pressure was statistically less than that in other cushions (P < 0.01). Use of wheelchair cushions was effective in redistribution of the pressure at ischium, and the overtime change in the pressure depends on the type of used cushions.

轮椅坐垫建议与轮椅一起使用,可以减轻轮椅使用者的界面压力,保护臀部免受疼痛和伤害。然而,随着新型轮椅坐垫的开发,还需要进行进一步的调查,以便正确使用轮椅坐垫。本研究的目的是通过比较四种坐垫的压力再分配效果来评估轮椅坐垫的物理特性。研究对象为 16 名同意参加本研究的健康成年人。测量使用了压力绘图系统(CONFORMat,Nitta 公司)。坐姿稳定后立即测量骶骨处的压力,10 分钟后测量骶骨处的压力。使用任何轮椅坐垫后,骶骨处的压力都明显下降(P < 0.01)。在未使用轮椅垫的情况下,体重指数与骶骨压力之间存在明显的负相关(r = - 0.70),但在使用轮椅垫后,这种相关性消失了。使用聚氨酯坐垫、空气坐垫和聚氨酯-空气混合坐垫时,骶骨处的压力会随着时间的推移而增加,而使用三维热塑弹性体坐垫时,骶骨处的压力不会增加,而且从统计学角度来看,压力的变化小于其他坐垫(P < 0.01)。使用轮椅坐垫能有效地重新分配骶骨处的压力,而压力的超时变化取决于所使用坐垫的类型。
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引用次数: 0
Antioxidant activity of Sophora exigua and liposome development of its powerful extract. 国槐的抗氧化活性及其强效提取物的脂质体开发
IF 1.9 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-09 Epub Date: 2024-05-22 DOI: 10.5582/ddt.2024.01018
Soraya Rodwattanagul, Mathurada Sasarom, Pornthida Riangjanapatee, Songyot Anuchapreeda, Siriporn Okonogi

Sophora exigua (SE) was sequentially extracted using hexane, ethyl acetate, and ethanol. The obtained extracts were tested for antioxidant activity. Among them, the fractionated ethyl acetate extract (SE-EA) showed the highest potential in free radical scavenging and ferric-reducing properties. The chemical analysis identified sophoraflavanone G as one of the active ingredients in SE-EA. According to SE-EA solubility, SE-EA liposomes were developed using a sonication-assisted thin film method. Cholesterol and phospholipids were used as the main compositions of the liposomes. The obtained liposomes were spherical with different nano-size ranges, size distribution, and zeta potential depending on SE-EA and total lipid concentrations. SE-EA liposomes were slightly bigger than their empty liposomes. All liposomes exhibited a phospholipid crystalline structure. Cholesterol and SE-EA existed in the liposomes as an amorphous state. SE-EA liposomes with high total lipid content exhibited high entrapment efficiency and sustained release behavior. Whereas liposomes with low total lipid content showed low entrapment efficiency and fast-release behavior. All SE-EA liposomes showed stronger antioxidant activity than the non-entrapped SE-EA. In conclusion, SE-EA is a natural source of potent antioxidants. The developed SE-EA liposomes are a promising pharmaceutical formulation to efficiently deliver the active ingredients of SE-EA and are suitable for further study in vivo.

Sophora exigua(SE)依次用正己烷、乙酸乙酯和乙醇提取。对获得的提取物进行了抗氧化活性测试。其中,分馏乙酸乙酯提取物(SE-EA)在清除自由基和还原铁元素方面表现出最高的潜力。化学分析确定槐黄烷酮 G 是 SE-EA 的活性成分之一。根据 SE-EA 的溶解性,采用超声辅助薄膜法开发了 SE-EA 脂质体。脂质体的主要成分是胆固醇和磷脂。所获得的脂质体呈球形,其纳米尺寸范围、尺寸分布和 Zeta 电位因 SE-EA 和总脂质浓度的不同而不同。SE-EA 脂质体比空脂质体略大。所有脂质体都呈现磷脂结晶结构。胆固醇和SE-EA以无定形状态存在于脂质体中。总脂质含量高的 SE-EA 脂质体具有较高的包载效率和持续释放特性。而总脂质含量低的脂质体则表现出较低的夹带效率和快速释放特性。与未包封的 SE-EA 相比,所有 SE-EA 脂质体都表现出更强的抗氧化活性。总之,SE-EA 是一种天然的强效抗氧化剂。所开发的 SE-EA 脂质体是一种很有前景的药物制剂,能有效地传递 SE-EA 的活性成分,适合在体内进行进一步研究。
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引用次数: 0
Subcutaneous edema as a potential cause of catheter failure in older inpatients receiving peripheral parenteral nutrition. 皮下水肿是导致接受外周肠外营养的老年住院患者导管失效的潜在原因。
IF 1.9 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-09 Epub Date: 2024-06-15 DOI: 10.5582/ddt.2024.01029
Motoko Kitada, Shigeo Yamamura, Etsuro Hori

Malnutrition is a common problem among hospitalized older patients. Peripheral parenteral nutrition (PN) can improve patient outcomes but can also lead to complications that affect future treatment. Older inpatients, in particular, are expected to be prone to these catheter-related complications. However, the impact of peripheral PN on older inpatients has been rarely investigated. In the current study, the impact of PN on short peripheral catheters (SPCs) was evaluated by comparing signs and symptoms at the time of catheter removal between 22 patients with PN and 27 without. In addition to external clinical assessment, sonographic investigations of the SPC site were performed. The prevalence of external signs and symptoms of complications was similar between the patients (all P > 0.05). However, subcutaneous edema was found by ultrasound in > 80% of patients with PN, compared with 55.6% of those without PN (P = 0.051). Unlike cases without PN, all patients with PN who presented with external signs and symptoms developed subcutaneous edema (P = 0.022). Multivariate analysis demonstrated that administration of PN was independently associated with subcutaneous edema (adjusted odds ratio = 6.88, 95% confidence interval = 1.083-75.486, P = 0.040). For several decades, phlebitis has been the primary focus of complications related to peripheral PN in clinical settings. However, our results imply that peripheral PN causes subcutaneous edema, which can lead to catheter failure in older inpatients. This study contributes to understanding the etiology of catheter failure during peripheral PN in this population.

营养不良是住院老年患者的常见问题。外周肠外营养(PN)可以改善患者的预后,但也可能导致影响未来治疗的并发症。老年住院患者尤其容易出现这些与导管相关的并发症。然而,外周 PN 对老年住院患者的影响却鲜有研究。在当前的研究中,通过比较 22 名有 PN 患者和 27 名无 PN 患者拔除导管时的体征和症状,评估了 PN 对短外周导管 (SPC) 的影响。除外部临床评估外,还对 SPC部位进行了超声检查。不同患者的并发症外部体征和症状发生率相似(均为 P > 0.05)。然而,超声波检查发现皮下水肿的 PN 患者超过 80%,而无 PN 患者为 55.6%(P = 0.051)。与无 PN 的病例不同,所有出现外部症状和体征的 PN 患者都出现了皮下水肿(P = 0.022)。多变量分析表明,使用 PN 与皮下水肿密切相关(调整赔率 = 6.88,95% 置信区间 = 1.083-75.486,P = 0.040)。几十年来,静脉炎一直是临床上外周静脉输液并发症的主要病因。然而,我们的研究结果表明,外周静脉输液会引起皮下水肿,从而导致老年住院患者导管失效。这项研究有助于了解老年患者外周静脉输液过程中导管失效的病因。
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引用次数: 0
Entamoeba moshkovskii as a potential model organism for Gal/GalNAc lectin intermediate subunit exhibition and functional identification. 将莫什科夫斯基恩塔米巴虫作为展示和鉴定 Gal/GalNAc 凝集素中间亚基功能的潜在模式生物。
IF 1.9 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2024-07-09 Epub Date: 2024-05-22 DOI: 10.5582/ddt.2024.01031
Qingshan Li, Meng Feng, Hongze Zhang, Hang Zhou, Xunjia Cheng

In humans, Entamoeba histolytica is the main pathogen causing various amoebiases, while E. moshkovskii falls between being a pathogen and non-pathogen. The two species have similar behavior patterns but differ significantly in pathogenicity, with previous studies and clinical data indicating that E. moshkovskii has a low level of pathogenicity. Meaningfully, the biological characteristics of E. moshkovskii make it a potential model organism and a protein display platform for studying the functions of important Entamoeba proteins. Here, an Amoeba-pcDNA3.1 vector capable of overexpressing E. histolytica-sourced Igl-C protein was constructed and successfully transfected into E. moshkovskii. High levels of expression of the Igl-C, EGFP, and NeoR genes were identified in Igl-C-transfected trophozoites using qRT-PCR, and they were subsequently confirmed using immunoblotting. Transfection of Igl-C protein improved the adherence and phagocytosis of E. moshkovskii, demonstrating that E. histolytica Igl mediated amoebic adhesion. Moreover, as a manifestation of protein virulence, the ability of post-transfected trophozoites to induce inflammation in host macrophages was also enhanced. In conclusion, this study utilizing the characteristics of E. moshkovskii confirmed its potential to serve as a model organism. E. moshkovskii could replace E. histolytica as the target of gene editing, allowing more efficient study of amoebic pathogenicity.

在人类中,组织溶解恩塔米巴虫是导致各种阿米巴病的主要病原体,而莫什科夫斯基恩塔米巴虫则介于病原体和非病原体之间。这两个物种具有相似的行为模式,但在致病性上有显著差异,以往的研究和临床数据表明,莫什科夫斯基伊氏菌的致病性较低。有意义的是,E. moshkovskii 的生物学特性使其成为研究重要恩塔米巴蛋白功能的潜在模式生物和蛋白质展示平台。在这里,我们构建了一种能够过表达来源于组织溶解虫的Igl-C蛋白的阿米巴-pcDNA3.1载体,并将其成功转染到莫什科夫斯基虫体内。利用 qRT-PCR 技术在转染 Igl-C 的滋养体中发现了 Igl-C、EGFP 和 NeoR 基因的高水平表达,随后利用免疫印迹技术对其进行了确认。转染Igl-C蛋白可提高E. moshkovskii的粘附性和吞噬能力,这表明组织溶解性伊丽莎白菌Igl介导了阿米巴粘附。此外,作为蛋白质毒力的一种表现,转染后滋养体诱导宿主巨噬细胞发炎的能力也得到了增强。总之,这项利用 E. moshkovskii 特性的研究证实了其作为模式生物的潜力。莫什科夫斯基伊蚊可以取代组织溶解伊蚊成为基因编辑的目标,从而更有效地研究阿米巴致病性。
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引用次数: 0
Need for a consensus definition of chronic dehydration: A scoping review. 需要对慢性脱水的定义达成共识:范围审查。
IF 3.1 Q1 Medicine Pub Date : 2024-06-06 Epub Date: 2024-04-25 DOI: 10.5582/ddt.2024.01014
Yoko Hasegawa, Katsunori Kato, Kazuhiro Ogai, Chizuko Konya, Takeo Minematsu

Dehydration is common in older adults and impacts their clinical outcomes. Chronic dehydration is especially important as it has been under-recognized. This scoping review aimed to summarize the available definitions of chronic dehydration to identify gaps between each definition and discuss future research needs. Four databases (Pubmed, CINAHL, Cochrane Library, Science Direct) were systematically searched for peer-reviewed articles that clearly described the definition of chronic dehydration published from inception to June 8th, 2023. Two researchers reviewed the articles independently, and any disagreement was solved upon discussion. We identified five articles with a wide range of subjects from children to older adults. Chronic dehydration was defined as a state of persistently elevated blood urea levels; weight loss ≥ 1% as a result of fluid loss; a ratio of blood urea nitrogen to creatinine > 20; serum osmolarity ≥ 295 mOsm/kg; and a dehydrated state lasting 72 hours or longer. The definition varied among studies, indicating the need to establish an international consensus on the definition of chronic dehydration.

脱水在老年人中很常见,会影响他们的临床治疗效果。慢性脱水尤其重要,因为人们对它的认识不足。本范围综述旨在总结现有的慢性脱水定义,找出每个定义之间的差距,并讨论未来的研究需求。研究人员在四个数据库(Pubmed、CINAHL、Cochrane Library、Science Direct)中系统地搜索了从开始到 2023 年 6 月 8 日发表的、明确描述了慢性脱水定义的同行评审文章。两名研究人员对文章进行了独立审阅,如有分歧,则通过讨论解决。我们确定了五篇文章,研究对象从儿童到老年人。慢性脱水的定义是:血尿素水平持续升高;因液体流失导致体重下降≥1%;血尿素氮与肌酐的比值大于 20;血清渗透压≥295 mOsm/kg;脱水状态持续 72 小时或更长时间。不同研究对慢性脱水的定义各不相同,这表明有必要就慢性脱水的定义达成国际共识。
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引用次数: 0
Bu-Shen-Ning-Xin decoction inhibits macrophage activation to ameliorate premature ovarian insufficiency-related osteoimmune disorder via FSH/FSHR pathway. 丙申宁心煎剂通过 FSH/FSHR 途径抑制巨噬细胞活化,改善卵巢早衰相关的骨免疫紊乱。
IF 3.1 Q1 Medicine Pub Date : 2024-06-06 Epub Date: 2024-04-17 DOI: 10.5582/ddt.2024.01006
Hongmei Sun, Qing Qi, Xinyao Pan, Jing Zhou, Jing Wang, Lisha Li, Dajing Li, Ling Wang

Limited studies are associated with premature ovarian insufficiency (POI)-related osteoimmune disorder currently. Bu-Shen-Ning-Xin decoction (BSNXD) displayed a favorable role in treating postmenopausal osteoporosis. However, its impact on the POI-related osteoimmune disorder remains unclear. The study primarily utilized animal experiments and network pharmacology to investigate the effects and underlying mechanisms of BSNXD on the POI-related osteoimmune disorder. First, a 4-vinylcyclohexene dioxide (VCD)-induced POI murine model was conducted to explore the therapeutical action of BSNXD. Second, we analyzed the active compounds of BSNXD and predicted their potential mechanisms for POI-related osteoimmune disorder via network pharmacology, further confirmed by molecular biology experiments. The results demonstrated that VCD exposure led to elevated follicle-stimulating hormone (FSH) levels, a 50% reduction in the primordial follicles, bone microstructure changes, and macrophage activation, indicating an osteoimmune disorder. BSNXD inhibited macrophage activation and osteoclast differentiation but did not affect serum FSH and estradiol levels in the VCD-induced POI model. Network pharmacology predicted the potential mechanisms of BSNXD against the POI-related osteoimmune disorder involving tumor necrosis factor α and MAPK signaling pathways, highlighting BSNXD regulated inflammation, hormone, and osteoclast differentiation. Further experiments identified BSNXD treatment suppressed macrophage activation via downregulating FSH receptor (FSHR) expression and inhibiting the phosphorylation of ERK and CCAAT enhancer binding proteins β. In conclusion, BSNXD regulated POI-related osteoimmune disorder by suppressing the FSH/FSHR pathway to reduce macrophage activation and further inhibiting osteoclastogenesis.

目前,与卵巢早衰(POI)相关的骨免疫疾病的研究有限。布参宁心汤(BSNXD)在治疗绝经后骨质疏松症方面发挥了良好的作用。然而,它对 POI 相关骨免疫疾病的影响仍不明确。本研究主要利用动物实验和网络药理学研究 BSNXD 对 POI 相关骨免疫疾病的影响及其内在机制。首先,通过 4-vinylcyclohexene dioxide(VCD)诱导的 POI 小鼠模型来探索 BSNXD 的治疗作用。其次,我们分析了 BSNXD 的活性化合物,并通过网络药理学预测了其治疗 POI 相关骨免疫性疾病的潜在机制,分子生物学实验进一步证实了这一点。结果表明,暴露于VCD会导致促卵泡激素(FSH)水平升高、原始卵泡减少50%、骨微结构改变和巨噬细胞活化,表明存在骨免疫紊乱。在 VCD 诱导的 POI 模型中,BSNXD 可抑制巨噬细胞活化和破骨细胞分化,但不影响血清 FSH 和雌二醇水平。网络药理学预测了BSNXD对抗POI相关骨免疫紊乱的潜在机制,其中涉及肿瘤坏死因子α和MAPK信号通路,强调了BSNXD对炎症、激素和破骨细胞分化的调节作用。总之,BSNXD 通过抑制 FSH/FSHR 通路来减少巨噬细胞的活化,并进一步抑制破骨细胞的生成,从而调节 POI 相关骨免疫疾病。
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引用次数: 0
Tuberculosis in pregnancy and assisted reproductive technology. 妊娠结核病和辅助生殖技术。
IF 3.1 Q1 Medicine Pub Date : 2024-06-06 Epub Date: 2024-04-17 DOI: 10.5582/ddt.2024.01007
Wenli Cao, Xiayan Fu, Haiyang Li, Jialu Bei, Lisha Li, Ling Wang

Tuberculosis is a chronic infectious disease caused by mycobacterium tuberculosis infection. In the world, tuberculosis is an important factor affecting women's reproductive health, which can cause reproductive tract anatomy abnormalities, embryo implantation obstacles, ovarian reserve and ovulation dysfunction, leading to female infertility. This group of women usually need to seek assisted reproductive technology to conceive. Latent tuberculosis infection during pregnancy has no clinical manifestation, but may develop into active tuberculosis, leading to adverse pregnancy outcomes. Most pregnant women do not need to be treated for latent tuberculosis infection, unless they are combined with high-risk factors for tuberculosis progress, but they need close follow-up. Early diagnosis and treatment of active tuberculosis in pregnancy can reduce the incidence rate and mortality of pregnant women and newborns, and treatment needs multidisciplinary cooperation.

结核病是由结核分枝杆菌感染引起的一种慢性传染病。在世界范围内,结核病是影响女性生殖健康的重要因素,可引起生殖道解剖结构异常、胚胎着床障碍、卵巢储备和排卵功能障碍,导致女性不孕。这类妇女通常需要寻求辅助生殖技术才能怀孕。孕期潜伏结核感染没有临床表现,但可能发展为活动性结核,导致不良妊娠结局。除非合并有结核病进展的高危因素,大多数孕妇不需要治疗潜伏结核感染,但需要密切随访。早期诊断和治疗妊娠期活动性结核病可以降低孕妇和新生儿的发病率和死亡率,治疗需要多学科合作。
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Drug Discoveries and Therapeutics
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