BMC Zoology最新文献

Background: The Caribbean spiny lobster Panulirus argus is heavily fished throughout its Greater Caribbean and Gulf of Mexico distribution, suggesting a heightened susceptibility to a fisheries collapse. In 2017, a nemertean worm, Carcinonemertes conanobrieni was described from ovigerous females of P. argus in Florida, USA. A year later, the presence of the same egg predator was recorded along the southern Caribbean coast (Colombia). The effect of this egg predator on the reproductive performance, including fecundity, embryo mortality, and reproductive output, of its host is unknown. This study tested whether C. conanobrieni affects embryo mortality, fecundity, and reproductive output in brooding females of P. argus.

Results: Artisan fishers caught 90 ovigerous lobsters near Pueblo Viejo, Magdalena, Colombia. Each ovigerous female was examined for the presence/absence of the egg predator. Lobster egg mortality (%), fecundity (nº eggs female^-1), and reproductive output (%) were estimated. Prevalence of C. conanobrieni in the studied population was 87.78%. The mean intensity of C. conanobrieni (all life stages) in the population was 11.68 (± 1.98) egg predators per brood mass sample. Infected females brooding late-stage embryos exhibited lower fecundity, lower reproductive performance values, and higher embryo mortality compared to infected females brooding early-stage embryos. Embryo stage and worm infection level negatively impacted fecundity and reproductive output. Worm infection level and the number of adult nemertean worms also negatively affected embryo mortality.

Conclusions: These results demonstrate an adverse effect of C. conanobrieni on the reproductive performance of P. argus. The interactive impact of this egg predator, natural stressors, and anthropogenic stressors on individual P. argus reproductive performance could facilitate losses at large-scale fisheries levels.

Human-wildlife interaction is a broad and complex topic. Due to rapid world population growth, there have been greater human impacts on wildlife through agriculture and land fragmentation. In many countries, significant challenges exist with managing wildlife and its negative impacts on humans and wildlife. This special issue discusses human-wildlife co-existence.

Background: The genus Nebo has been identified as a medically important scorpion species distributed across Arabia and the Middle East. However, its taxonomic status remains unclear.

Aim: The molecular phylogeny of two Nebo species from Saudi Arabia and comparative sequences from Palestine is presented based on the mitochondrial cytochrome oxidase subunit I (COI) gene.

Methodology: Scorpion specimens were collected from two different localities, mainly the Southern part of Saudi Arabia. Then, DNA was extracted, amplified using invertebrate universal primers, and sequenced to identify the COI gene. The obtained sequences were analyzed, and phylogenetic trees based on maximum parsimony, neighbor-joining, and Bayesian inference were constructed.

Results: The inferred phylogeny indicates the monophyletic status of the family Diplocentridae and its subfamily Nebinae and Diplocentrinae. Also, the phylogenetic analyses support the existence of interspecific and intraspecific variations among/ within Nebo hierichonticus and Nebo yemenensis which may indicate distinct species.

Conclusion: Further morphological studies with additional specimens from the Arabian Peninsula may reveal possible undiscovered and cryptic species.

Wetlands are significant habitats for bird populations, and knowledge of the diversity and other ecological aspects of bird species contribute to the management of the ecosystem. The present study was based on comparative studies of the diversity and relative abundance of bird species in the two wetlands of southwest Ethiopia. The point count method was utilized in this study. For the data analysis, the Shannon-Weaver diversity index, independent sample t-test, and similarity index were employed. A total of 36 bird species under 11 orders and 24 families were identified. The species diversity and relative abundance were higher in both wetlands during the wet season. The Loga wetland had the higher diversity (H' = 3.089), whereas the lowest species diversity (H' = 2.643) was recorded in the wetland of Hurri. During the dry season, the Loga wetland (H' = 2.738) and the Hurri habitat (H' = 2.283) had higher and lower diversity, respectively. Seasonal variations in bird species diversity are not statistically significant (p > 0.05). Although the two wetlands support several water birds, they have received no conservation attention from concerned bodies. Further follow-up studies over a long period will help determine species-specific conservation measures for wetland-dependent birds.

Amphibians are facing population declines and extinctions, and protecting and supplementing refuges can help species survive. However, the microhabitat requirements of most species are unknown, and artificial shelters or burrows have not been well tested for amphibians. Some amphibians exhibit complex behaviour during the transition from post-reproductive dormancy to activity. However, little is known about the ecology, post-reproductive dormancy, and terrestrial activity of amphibians. Here, habitat site selection in experimental enclosures and the effects of shelters (stones, soil) and shade (with and without shade netting) on the activity, exposed body percentage, burrow depth, body-soil contact percentage, and survival of Rana dybowskii were investigated during post-reproductive dormancy and post-dormant activity. The results showed that R. dybowskii live individually under leaves, soil, stones or tree roots. Furthermore, although the dormant sites of frogs are significantly different, the distribution of male and female frogs in these sites is similar. Shading and shelter significantly affected the exposed body percentage, burrow depth and body-soil contact percentage of frogs compared with soil. In the stone group, soil and stone form the frog's refuge/burrow, whereas in the soil group, the refuge/burrow is composed entirely of soil. Even though the soil group has a deeper burrow and a larger area of soil contact with the body, it still has a higher exposure rate than the stone group. Frog activity frequency was affected by shelter and shade; the interaction of shelter and time and the interaction of shading and time were significant. The soil group had a higher activity frequency than the stone group, and the no-shade group had a higher activity frequency than the shade group. Shelter and shading differences do not significantly affect frog survival; however, the death rate during post-reproductive dormancy is lower than that during the active period.

Background: Gallinula chloropus (Linnaeus, 1758) is a wild aquatic omnivorous bird characterized by a marked resistance to harsh environmental conditions and a worldwide distribution. In this study, anatomical, morphometrical, histochemical, and immunohistochemical techniques were employed to study the structure of the gastrointestinal tract of Gallinula chloropus.

Results: The esophagus appeared tubular with no distinct crop. Both superficial (SPG) and deep (DPG) proventricular glands were present. The DPG filled about two-thirds of the total wall thickness. Histochemically, the mucosubstances revealed mixed alcian blue-PAS positive reactions. They were mainly localized in the acini of the esophageal glands and SPG, gastric surface epithelium, duct system of DPG, and intestinal goblet cells. The highest number of goblet cells per every 1 mm² of the intestinal mucosa was seen within the ileum and rectum, 2555 ± 468 and 2607 ± 653 respectively. Notably, glucagon immunoreactive (IR) cells were abundant in the mucosa of the small and large intestines and the proventriculus, while somatostatin IR cells were concentrated within the acini of the DPG. IR cells for the mitosis marker phospho-histone H3 (PHH3) were highest within the entire intestinal crypts and mucosa-associated lymphoid tissues (MALT). In contrast, cells IR for the apoptosis marker C.CASP3 were remarkable in epithelial cells at the tips of intestinal villi and in MALT, reflecting the dynamic nature of the latter mentioned structures.

Conclusions: The findings of the present study advance our knowledge of the gross and microscopic anatomy of the gastrointestinal tract in wild birds and could help to enhance the productivity of Aves via improving gut health.

Background: In molecular biology studies, the selection of optimal reference genes is of vital importance for accurately quantifying gene expression. The purpose of the present study was to screen the most stable reference genes in different muscle tissues of New Zealand white rabbits and Yufeng yellow rabbits.

Methods and results: Results indicated that the most stable reference genes in the muscle tissues of New Zealand white rabbits were HPRT1, ACTB and PPIC, while HPRT1, PPIC, and RPL13A were the most stable reference genes in muscle tissues of Yufeng yellow rabbits. However, in the longissimus dorsi muscle and the abdominal wall muscle of both varieties, the most stable reference genes were HPRT1, RPL13A, and SDHA. In the quadriceps femoris muscle, the most stable reference genes were ACTB, HPRT1, and SDHA. Furthermore, the relative abundance of MYOG, MYH3 and MSTN was used to confirm the suitability and reliability of the selected most stable reference genes and the most unstable reference gene. Results revealed the same expression patterns of these myogenic genes when normalized according to the most stable genes, while normalization against the unstable reference gene altered the observed expression patterns.

Conclusions: Taken together, our results demonstrated that the most stable reference genes varied among different muscle tissues and different breeds of rabbits. However, HPRT1, PPIC and SDHA presented high stability among all examined reference genes; thus, the combined analysis of HPRT1/ PPIC/ SDHA gene provides the best reference for RT-qPCR in muscle tissues of New Zealand white rabbits and Yufeng yellow rabbits, while HPRT1 is a better choice than other reference genes when using a single reference gene to assess target gene expression. Our results provide basic data for better measuring target gene expression profiles in muscle tissues of rabbits.

Background: Animals use contests to attain resources and employ strategic decisions to minimise contest costs. These decisions are defined by behavioural response to resource value and competitive ability, but remain poorly understood. This is because the two factors are typically studied separately. Also, their study relies on overgeneralised assumptions that (i) strategies are fixed, (ii) modulated by the motivation or drive to fight and (iii) used to manage costs proportional to the timing of the loser's retreat. To address these problems, we adopt an integrative sequential analysis that incorporates competitive ability and resource value factors, to characterise territorial contest decisions in male Siamese fighting fish (Betta splendens).

Results: Individuals exhibited a chronological organisation of behaviour, engaging opponents first with frontal display, then switching to lateral display before deciding to attack, and reserved retreats for later stages. Using asymmetries in retreats as a proxy for outcome, the likelihood of winning was found to be mostly dependent on display. However, resource and contest conditions affected initiation latency, display, attack and retreat, suggesting that strategic decisions influence all behaviour. Overall, sequential behaviour varied consistently with individual aggressiveness and resource-value factors, and increasingly with information on competitive ability collected during the contest. This enabled shifts in tactics, such as disadvantaged individuals responding first with aggression and later with submission. Motivation to continue fighting, after interruption by startle, was also adjusted to information gathered during the contest and progressively with energetic state. Two clusters of correlated behaviours were identified, cost-mitigation (display and retreat) and escalation (initiation and attack), but changes in motivation were associated only with cost mitigation.

Conclusions: Our findings contrast dominant assumptions that strategic decisions are fixed, controlled by motivational state and sufficiently described by outcome-dependent measures. We instead demonstrate that strategic decisions are complex, comprising functional changes in assessment, information use and motivational effects, which are not always inter-dependent.