Pub Date : 2022-05-19DOI: 10.1080/20442041.2022.2077633
A. Kuranchie, D. Anim, A. Harmer, D. Brunton
ABSTRACT Understanding the spatial relationships between land use/land cover (LULC) and physicochemical water quality in pond ecosystems is vital to the conservation and management of ponds. This knowledge is especially critical to reconcile landscape planning and management, particularly in urban regions with rapid population growth. In this study we measured (1) seasonal differences in water quality and (2) the impact of the surrounding landscape at 4 spatial scales (10 m, 100 m, 500 m, and full catchment) on water quality of 50 ponds in the Auckland region, New Zealand. For each pond, 9 physicochemical water quality variables and 9 landscape properties (LULC and physical features of the ponds) were measured in winter and summer. We found significantly higher measures of conductivity, total dissolved solids, percentage of dissolved oxygen at saturation (%DO), pH, salinity, and phosphate concentrations in summer. By contrast, ammoniacal nitrogen concentration was higher in winter. These findings indicated impaired water quality during summer. Multiple linear regression and redundancy analyses showed that LULC and physical landscape features had different influences on the physicochemical variables across the different spatial scales and seasons. The landscape properties at all 4 spatial scales were good indicators of pond temperature and %DO only in summer. Overall, variations in pond water quality were explained better by general landscape characteristics than by LULC alone at the catchment and 500 m scale in winter and at the 100 m scale in summer. This study highlights the importance of including different spatial scales, seasons, and landscapes when quantifying land–water interactions.
{"title":"The influence of season and landscape on the water quality of ponds at multiple spatial scales","authors":"A. Kuranchie, D. Anim, A. Harmer, D. Brunton","doi":"10.1080/20442041.2022.2077633","DOIUrl":"https://doi.org/10.1080/20442041.2022.2077633","url":null,"abstract":"ABSTRACT Understanding the spatial relationships between land use/land cover (LULC) and physicochemical water quality in pond ecosystems is vital to the conservation and management of ponds. This knowledge is especially critical to reconcile landscape planning and management, particularly in urban regions with rapid population growth. In this study we measured (1) seasonal differences in water quality and (2) the impact of the surrounding landscape at 4 spatial scales (10 m, 100 m, 500 m, and full catchment) on water quality of 50 ponds in the Auckland region, New Zealand. For each pond, 9 physicochemical water quality variables and 9 landscape properties (LULC and physical features of the ponds) were measured in winter and summer. We found significantly higher measures of conductivity, total dissolved solids, percentage of dissolved oxygen at saturation (%DO), pH, salinity, and phosphate concentrations in summer. By contrast, ammoniacal nitrogen concentration was higher in winter. These findings indicated impaired water quality during summer. Multiple linear regression and redundancy analyses showed that LULC and physical landscape features had different influences on the physicochemical variables across the different spatial scales and seasons. The landscape properties at all 4 spatial scales were good indicators of pond temperature and %DO only in summer. Overall, variations in pond water quality were explained better by general landscape characteristics than by LULC alone at the catchment and 500 m scale in winter and at the 100 m scale in summer. This study highlights the importance of including different spatial scales, seasons, and landscapes when quantifying land–water interactions.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"477 - 487"},"PeriodicalIF":3.1,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46064993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-29DOI: 10.1080/20442041.2022.2067464
Xinchen Wang, Hong Zhang, E. Bertone, R. Stewart, S. P. Hughes
ABSTRACT A coupled data-driven and 3-dimensional (3D) process-based fluorescent dissolved organic matter (fDOM) prediction model was developed for a shallow, subtropical Australian reservoir. The extent to which reservoir water volume, inflow, and wind conditions affect the fDOM transport dynamics during cyclonic weather events was assessed through scenario analysis and a data-driven Bayesian network (BN) approach. The analysis shows that (a) inflow plumes are the main sources of fDOM during heavy rainfall; (b) the concentration of fDOM near the dam wall is related to rainfall intensity; (c) higher reservoir volumes reduce the rate of increase and peak of fDOM concentration during rainfall events; and (d) fDOM transport to the dam wall is strongly influenced by the prevailing wind direction. A naïve BN developed for fDOM assessment displayed a strong sensitivity of the peak fDOM value to rainfall-related characteristics while the lag time between rainfall event and fDOM peak at the dam wall was highly sensitive to reservoir water volume and wind speed. The hybrid modelling approach provides both new information on 3D fDOM transport in reservoirs during extreme weather events through the model application and an easy-to-interpret, instantaneous modelling output for treatment operators through the BN modelling component. The BN modelling is an essential addition for water treatment operators to promptly predict the impacts of extreme weather events and proactively adjust treatment operations without the computational time burden of a 3D process-based model.
{"title":"Hybrid three-dimensional modelling for reservoir fluorescent dissolved organic matter risk assessment","authors":"Xinchen Wang, Hong Zhang, E. Bertone, R. Stewart, S. P. Hughes","doi":"10.1080/20442041.2022.2067464","DOIUrl":"https://doi.org/10.1080/20442041.2022.2067464","url":null,"abstract":"ABSTRACT A coupled data-driven and 3-dimensional (3D) process-based fluorescent dissolved organic matter (fDOM) prediction model was developed for a shallow, subtropical Australian reservoir. The extent to which reservoir water volume, inflow, and wind conditions affect the fDOM transport dynamics during cyclonic weather events was assessed through scenario analysis and a data-driven Bayesian network (BN) approach. The analysis shows that (a) inflow plumes are the main sources of fDOM during heavy rainfall; (b) the concentration of fDOM near the dam wall is related to rainfall intensity; (c) higher reservoir volumes reduce the rate of increase and peak of fDOM concentration during rainfall events; and (d) fDOM transport to the dam wall is strongly influenced by the prevailing wind direction. A naïve BN developed for fDOM assessment displayed a strong sensitivity of the peak fDOM value to rainfall-related characteristics while the lag time between rainfall event and fDOM peak at the dam wall was highly sensitive to reservoir water volume and wind speed. The hybrid modelling approach provides both new information on 3D fDOM transport in reservoirs during extreme weather events through the model application and an easy-to-interpret, instantaneous modelling output for treatment operators through the BN modelling component. The BN modelling is an essential addition for water treatment operators to promptly predict the impacts of extreme weather events and proactively adjust treatment operations without the computational time burden of a 3D process-based model.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"463 - 476"},"PeriodicalIF":3.1,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41461489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-11DOI: 10.1080/20442041.2022.2063623
A. Michaud, S. Apollonio
ABSTRACT Arctic lakes are ice covered for 8–12 months of the year, but the duration and thickness of ice cover is decreasing with increased warming. There is a paucity of baseline data documenting the geochemical dynamics of nutrients and oxygen beneath ice cover of high Arctic lakes, a gap that presents a challenge when attempting to understand the impacts of a rapidly changing Arctic on lake ecosystems. We present an annual cycle of temperature, oxygen, and silicate data from Immerk Lake on Truelove Lowland, Devon Island, Canada. Water column oxygen concentrations decreased while silicate concentrations increased during the ice-covered season. Ice cover melting during spring–summer is associated with rapid net decreases of the silicate standing stock at almost 3 times the rate of its net increase over winter. These data show the importance of the extended winter season for regeneration of silicate, an essential nutrient for diatom populations, which are important members of the phytoplankton community and carbon cycle of Arctic lakes. These data collected 60 years ago serve as a benchmark and document the water column oxygen and silicate dynamics in an Arctic lake during ice formation, winter darkness, and ice-cover melting periods.
{"title":"Overwinter oxygen and silicate dynamics in a high Arctic lake (Immerk Lake, Devon Island, Canada)","authors":"A. Michaud, S. Apollonio","doi":"10.1080/20442041.2022.2063623","DOIUrl":"https://doi.org/10.1080/20442041.2022.2063623","url":null,"abstract":"ABSTRACT Arctic lakes are ice covered for 8–12 months of the year, but the duration and thickness of ice cover is decreasing with increased warming. There is a paucity of baseline data documenting the geochemical dynamics of nutrients and oxygen beneath ice cover of high Arctic lakes, a gap that presents a challenge when attempting to understand the impacts of a rapidly changing Arctic on lake ecosystems. We present an annual cycle of temperature, oxygen, and silicate data from Immerk Lake on Truelove Lowland, Devon Island, Canada. Water column oxygen concentrations decreased while silicate concentrations increased during the ice-covered season. Ice cover melting during spring–summer is associated with rapid net decreases of the silicate standing stock at almost 3 times the rate of its net increase over winter. These data show the importance of the extended winter season for regeneration of silicate, an essential nutrient for diatom populations, which are important members of the phytoplankton community and carbon cycle of Arctic lakes. These data collected 60 years ago serve as a benchmark and document the water column oxygen and silicate dynamics in an Arctic lake during ice formation, winter darkness, and ice-cover melting periods.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"418 - 426"},"PeriodicalIF":3.1,"publicationDate":"2022-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41364378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-28DOI: 10.1080/20442041.2022.2058861
Huanan Gao, Xin Liu, S. Ban
ABSTRACT Acute acidic stress from sources such as acid rain might impact lake ecosystems in many aspects. Because zooplankton are sensitive to environmental change, they could be a good biotic indicator of acidification effects. We investigated survival and metabolic rates of 3 dominant zooplankton taxa in Lake Biwa, the cladoceran Daphnia pulicaria and the copepods Eodiaptomus japonicus and Cyclopoida spp., when subjected to different pH values (pH 4.0–8.0) at low (10 °C) and high (20 °C) temperatures. Because mortality of D. pulicaria and Cyclopoida spp. exceeded 50% over 24 h of incubation at pH < 4.6, but for E. japonicus at pH < 5.6, E. japonicus may be more vulnerable to acidic stress than the other 2 taxa. This vulnerability to acidification slightly increased at 10 °C for each taxon. Metabolic rates in D. pulicaria remained relatively constant over a wide pH range (4.6–8.0), but those of both copepods decreased at acidic conditions (pH 4.6–7.0). This decrease of metabolic rate might be related to their swimming activity, which decreased in acidic conditions.
{"title":"Effect of acute acidic stress on survival and metabolic activity of zooplankton from Lake Biwa, Japan","authors":"Huanan Gao, Xin Liu, S. Ban","doi":"10.1080/20442041.2022.2058861","DOIUrl":"https://doi.org/10.1080/20442041.2022.2058861","url":null,"abstract":"ABSTRACT Acute acidic stress from sources such as acid rain might impact lake ecosystems in many aspects. Because zooplankton are sensitive to environmental change, they could be a good biotic indicator of acidification effects. We investigated survival and metabolic rates of 3 dominant zooplankton taxa in Lake Biwa, the cladoceran Daphnia pulicaria and the copepods Eodiaptomus japonicus and Cyclopoida spp., when subjected to different pH values (pH 4.0–8.0) at low (10 °C) and high (20 °C) temperatures. Because mortality of D. pulicaria and Cyclopoida spp. exceeded 50% over 24 h of incubation at pH < 4.6, but for E. japonicus at pH < 5.6, E. japonicus may be more vulnerable to acidic stress than the other 2 taxa. This vulnerability to acidification slightly increased at 10 °C for each taxon. Metabolic rates in D. pulicaria remained relatively constant over a wide pH range (4.6–8.0), but those of both copepods decreased at acidic conditions (pH 4.6–7.0). This decrease of metabolic rate might be related to their swimming activity, which decreased in acidic conditions.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"488 - 498"},"PeriodicalIF":3.1,"publicationDate":"2022-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49606317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-25DOI: 10.1080/20442041.2022.2057164
W. Tsoi, I. Growns, M. Southwell, S. Mika, Sam Lewis, D. Ryder, P. Frazier
ABSTRACT Floodplain wetlands play a significant role in the storage of sediment and water and support high levels of nutrient cycling driven by intermittent inundation. In regulated rivers, the frequency and duration of floodplain inundation are often reduced. Managed water inundation is used as a tool to help restore floodplains, but its outcome on wetlands requires further quantification. We examined the effects of environmental floodplain watering on water quality and 3 groups of invertebrates, including benthic and pelagic microinvertebrates and macroinvertebrates, in 2 wetlands systems on the Gwydir River system in the north of the Murray-Darling Basin. We hypothesised that a wetland inundated for longer periods would alter water quality and support a greater richness and abundance of invertebrates, thus altering their assemblage structures. Water quality and the assemblage structure of all 3 invertebrate groups in the wetlands were significantly influenced by the time since connection (TSC) to their rivers and therefore the length of inundation. However, the response of water quality and the microinvertebrate assemblages to TSC differed between the 2 wetlands. Water quality was affected by an increase in 6 variables, including total nitrogen, and a decrease in soluble reactive phosphorus. Microinvertebrate abundance was positively associated with TSC, but the abundance of macroinvertebrates was not. The relationships demonstrated between TSC and invertebrates indicate that the duration of inundation is important for ecological structure and food webs in these and other semiarid floodplain wetlands.
{"title":"Effects of inundation on water quality and invertebrates in semiarid floodplain wetlands","authors":"W. Tsoi, I. Growns, M. Southwell, S. Mika, Sam Lewis, D. Ryder, P. Frazier","doi":"10.1080/20442041.2022.2057164","DOIUrl":"https://doi.org/10.1080/20442041.2022.2057164","url":null,"abstract":"ABSTRACT Floodplain wetlands play a significant role in the storage of sediment and water and support high levels of nutrient cycling driven by intermittent inundation. In regulated rivers, the frequency and duration of floodplain inundation are often reduced. Managed water inundation is used as a tool to help restore floodplains, but its outcome on wetlands requires further quantification. We examined the effects of environmental floodplain watering on water quality and 3 groups of invertebrates, including benthic and pelagic microinvertebrates and macroinvertebrates, in 2 wetlands systems on the Gwydir River system in the north of the Murray-Darling Basin. We hypothesised that a wetland inundated for longer periods would alter water quality and support a greater richness and abundance of invertebrates, thus altering their assemblage structures. Water quality and the assemblage structure of all 3 invertebrate groups in the wetlands were significantly influenced by the time since connection (TSC) to their rivers and therefore the length of inundation. However, the response of water quality and the microinvertebrate assemblages to TSC differed between the 2 wetlands. Water quality was affected by an increase in 6 variables, including total nitrogen, and a decrease in soluble reactive phosphorus. Microinvertebrate abundance was positively associated with TSC, but the abundance of macroinvertebrates was not. The relationships demonstrated between TSC and invertebrates indicate that the duration of inundation is important for ecological structure and food webs in these and other semiarid floodplain wetlands.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"397 - 406"},"PeriodicalIF":3.1,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48305847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-18DOI: 10.1080/20442041.2022.2052785
Z. Simpson, R. W. McDowell, L. Condron
ABSTRACT Streams can attenuate inputs of phosphorus (P) and therefore dampen the likelihood of ecosystem eutrophication. This P attenuation derives from many processes and remains poorly understood, particularly in reference to the geochemical mechanisms involved. We studied P attenuation in the form of (1) potential for mineral (co-)precipitation from the water column and (2) P sorption with benthic sediments. We hypothesized that both mechanisms vary with catchment geology and that P sorption depends on sediment reactive iron (Fe). We sampled 31 streams at baseflow, covering a gradient of P inputs (via land use), hydrological characteristics, and catchment geologies. Geochemical equilibria in the water column were measured and benthic sediments were analyzed for sorption properties and P and Fe fractions. Neither P-containing minerals nor calcite-phosphate co-precipitation had the potential to form. However, in-stream dissolved reactive P (DRP) correlated with labile sediment P (water soluble and easily reduced Fe-P) for streams where hyporheic exchange between the water column and sediment porewaters was likely sufficient. Because this labile P was associated with poorly crystalline Fe oxides, which determined P sorption capacity, we observed that more sorptive sediments were positively related to DRP concentrations. Our results suggest that DRP attenuation in these streams at baseflow depends in part on the combination of biogeochemical Fe and P cycles and the hydrological exchange with the hyporheic zone. Such combinations will likely vary spatiotemporally within a catchment and must be considered alongside inputs of P and sediment if the P concentrations at baseflow, and eutrophication risk, are to be well managed.
{"title":"Sediment and water-column phosphorus chemistry in streams at baseflow across varying catchment geologies","authors":"Z. Simpson, R. W. McDowell, L. Condron","doi":"10.1080/20442041.2022.2052785","DOIUrl":"https://doi.org/10.1080/20442041.2022.2052785","url":null,"abstract":"ABSTRACT Streams can attenuate inputs of phosphorus (P) and therefore dampen the likelihood of ecosystem eutrophication. This P attenuation derives from many processes and remains poorly understood, particularly in reference to the geochemical mechanisms involved. We studied P attenuation in the form of (1) potential for mineral (co-)precipitation from the water column and (2) P sorption with benthic sediments. We hypothesized that both mechanisms vary with catchment geology and that P sorption depends on sediment reactive iron (Fe). We sampled 31 streams at baseflow, covering a gradient of P inputs (via land use), hydrological characteristics, and catchment geologies. Geochemical equilibria in the water column were measured and benthic sediments were analyzed for sorption properties and P and Fe fractions. Neither P-containing minerals nor calcite-phosphate co-precipitation had the potential to form. However, in-stream dissolved reactive P (DRP) correlated with labile sediment P (water soluble and easily reduced Fe-P) for streams where hyporheic exchange between the water column and sediment porewaters was likely sufficient. Because this labile P was associated with poorly crystalline Fe oxides, which determined P sorption capacity, we observed that more sorptive sediments were positively related to DRP concentrations. Our results suggest that DRP attenuation in these streams at baseflow depends in part on the combination of biogeochemical Fe and P cycles and the hydrological exchange with the hyporheic zone. Such combinations will likely vary spatiotemporally within a catchment and must be considered alongside inputs of P and sediment if the P concentrations at baseflow, and eutrophication risk, are to be well managed.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"510 - 525"},"PeriodicalIF":3.1,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45726884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-18DOI: 10.1080/20442041.2021.2015994
S. Guildford, R. Hecky, P. Verburg, Anathea Albert
ABSTRACT Lakes on volcanic soils of the North Island of New Zealand, compared to north temperate lakes, have low total nitrogen:total phosphorus (TN:TP) ratios, low dissolved inorganic nitrogen concentrations during summer stratification, and have positive chlorophyll a responses to N additions more frequently than to P in nutrient enrichment bioassays. This response has resulted, in some cases, to the imposition of N loading caps on some lake catchments, in contrast to nutrient management in north temperate lakes focused more on P management. To explore this possible difference in limiting nutrients, a suite of nutrient status indicators based on measurement of ambient seston stoichiometry and metabolic activity, which have been widely used in north temperate lakes, were applied to 8 lakes on volcanic terrain with previously reported N limitation. These nutrient status measurements were previously calibrated to inform whether in situ phytoplankton are growth-rate limited and, if so, which nutrient is limiting growth rate. In austral summer 2015, all 8 lakes showed indications of P limitation, and P limitation was more extreme, pervasive, and persistent (among samplings) than N limitation. Indications of N limitation were not observed without contemporaneous evidence of P limitation, but P limitation was frequently observed without evidence of N limitation. One lake, Rotorua, was not strongly nutrient limited, and phytoplankton were likely growing at or near optimum growth rates. In this study the commonly used TN:TP ratio was a poor predictor of which nutrient was potentially limiting in situ phytoplankton.
{"title":"Phosphorus limitation in low nitrogen lakes in New Zealand","authors":"S. Guildford, R. Hecky, P. Verburg, Anathea Albert","doi":"10.1080/20442041.2021.2015994","DOIUrl":"https://doi.org/10.1080/20442041.2021.2015994","url":null,"abstract":"ABSTRACT Lakes on volcanic soils of the North Island of New Zealand, compared to north temperate lakes, have low total nitrogen:total phosphorus (TN:TP) ratios, low dissolved inorganic nitrogen concentrations during summer stratification, and have positive chlorophyll a responses to N additions more frequently than to P in nutrient enrichment bioassays. This response has resulted, in some cases, to the imposition of N loading caps on some lake catchments, in contrast to nutrient management in north temperate lakes focused more on P management. To explore this possible difference in limiting nutrients, a suite of nutrient status indicators based on measurement of ambient seston stoichiometry and metabolic activity, which have been widely used in north temperate lakes, were applied to 8 lakes on volcanic terrain with previously reported N limitation. These nutrient status measurements were previously calibrated to inform whether in situ phytoplankton are growth-rate limited and, if so, which nutrient is limiting growth rate. In austral summer 2015, all 8 lakes showed indications of P limitation, and P limitation was more extreme, pervasive, and persistent (among samplings) than N limitation. Indications of N limitation were not observed without contemporaneous evidence of P limitation, but P limitation was frequently observed without evidence of N limitation. One lake, Rotorua, was not strongly nutrient limited, and phytoplankton were likely growing at or near optimum growth rates. In this study the commonly used TN:TP ratio was a poor predictor of which nutrient was potentially limiting in situ phytoplankton.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"261 - 276"},"PeriodicalIF":3.1,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43953618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-03-18DOI: 10.1080/20442041.2022.2053455
Lena A Schallenberg, S. Wood, J. Puddick, P. J. Cabello-Yeves, C. Burns
ABSTRACT Freshwater picocyanobacteria form the base of microbial food webs in many lakes worldwide but have received less attention than other phytoplankton. Little is known about their potential response to environmental changes such as increased nutrient loading and climate change, due partly to the lack of available cultured and sequenced strains. Here, we isolated 25 monoclonal picocyanobacterial strains from 6 New Zealand lakes with contrasting trophic states. The use of MLA medium instead of BG11 proved highly successful for the rapid isolation of picocyanobacteria. Strains were characterised by sequencing of the 16S ribosomal RNA gene, spectrophotometry, and high-performance liquid chromatography. 16S rRNA gene analysis placed most strains within the cluster 5 picocyanobacterial lineage (sub-cluster 5.2, family: Synechococcaceae). Phylogenetic analysis showed that 12 isolates from Lakes Wakatipu, Hayes, Johnson, and Ellesmere/Te Waihora clustered with strains from a range of Northern Hemisphere locations, suggesting global dispersal of these strains. Pigment characterisation revealed that pink and brown cultures from oligotrophic and some eutrophic lakes were rich in phycoerythrin, while green cultures from eutrophic and hypertrophic lakes were rich in phycocyanin. This diverse group of freshwater cluster 5 picocyanobacterial cultures will provide a new resource to study how these critically important microbes function and respond to changing environmental stressors.
{"title":"Isolation and characterisation of monoclonal picocyanobacterial strains from contrasting New Zealand lakes","authors":"Lena A Schallenberg, S. Wood, J. Puddick, P. J. Cabello-Yeves, C. Burns","doi":"10.1080/20442041.2022.2053455","DOIUrl":"https://doi.org/10.1080/20442041.2022.2053455","url":null,"abstract":"ABSTRACT Freshwater picocyanobacteria form the base of microbial food webs in many lakes worldwide but have received less attention than other phytoplankton. Little is known about their potential response to environmental changes such as increased nutrient loading and climate change, due partly to the lack of available cultured and sequenced strains. Here, we isolated 25 monoclonal picocyanobacterial strains from 6 New Zealand lakes with contrasting trophic states. The use of MLA medium instead of BG11 proved highly successful for the rapid isolation of picocyanobacteria. Strains were characterised by sequencing of the 16S ribosomal RNA gene, spectrophotometry, and high-performance liquid chromatography. 16S rRNA gene analysis placed most strains within the cluster 5 picocyanobacterial lineage (sub-cluster 5.2, family: Synechococcaceae). Phylogenetic analysis showed that 12 isolates from Lakes Wakatipu, Hayes, Johnson, and Ellesmere/Te Waihora clustered with strains from a range of Northern Hemisphere locations, suggesting global dispersal of these strains. Pigment characterisation revealed that pink and brown cultures from oligotrophic and some eutrophic lakes were rich in phycoerythrin, while green cultures from eutrophic and hypertrophic lakes were rich in phycocyanin. This diverse group of freshwater cluster 5 picocyanobacterial cultures will provide a new resource to study how these critically important microbes function and respond to changing environmental stressors.","PeriodicalId":49061,"journal":{"name":"Inland Waters","volume":"12 1","pages":"383 - 396"},"PeriodicalIF":3.1,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48711374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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