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A 'Regent' pedigree update: ancestors, offspring and their confirmed resistance loci “Regent”谱系更新:祖先、后代及其已确认的抗性基因座
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-10-26 DOI: 10.5073/VITIS.2021.60.189-193
Franco Röckel, O. Trapp, E. Zyprian, L. Hausmann, D. Migliaro, S. Vezzulli, R. Töpfer, E. Maul
'Regent' is the fungal resistant grapevine cultivar with the highest acreage in Germany and an important resistance donor in international breeding programs. It carries the resistance loci Rpv3.1 as well as Ren3 and Ren9 against downy and powdery mildew, respectively. As the parents of 'Chambourcin', the resistant paternal ancestor of 'Regent', did not coincide with the breeder's information, the germplasm repository of JKI Geilweilerhof was screened to find the missing ancestors. SSR marker analysis revealed that 'Joannes Seyve 11369' and 'Plantet' are the true parents of 'Chambourcin' and not 'Seyve Villard 12-417' and 'Chancellor'. Furthermore, the origin of the resistance loci Ren3 and Ren9 could be traced back to the genotypes 'Seibel 4614' and 'Munson'. Since the breeder Hermann Jaeger mentioned 'Munson' as a direct descendant of Vitis aestivalis Michx. var. linsecomii (Buckley) L. H. Bailey and Vitis rupestris Scheele, one of these wild species might have been the donor of the loci.
“Regent”是德国种植面积最高的抗真菌葡萄品种,也是国际育种计划中的重要抗真菌供体。它携带的抗性位点Rpv3.1以及Ren3和Ren9分别对霜霉病和白粉菌。由于“Regent”的抗性父系祖先“Chambourcin”的父母与育种家的信息不一致,因此对JKI Geilweilerhof的种质库进行了筛选,以寻找缺失的祖先。SSR标记分析表明,“Joannes Seyve 11369”和“Planet”是“Chambourcin”的真正父母,而不是“Seyve Villard 12-417”和“Chancellor”。此外,抗性基因座Ren3和Ren9的起源可以追溯到基因型“Seibel 4614”和“Munson”。由于饲养员Hermann Jaeger提到“Munson”是葡萄的直系后裔。这些野生物种中的一个可能是基因座的供体。
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引用次数: 4
High-resolution 3D phenotyping of the grapevine root system using X-ray Computed Tomography 应用X射线计算机断层扫描技术对葡萄根系进行高分辨率三维表型分析
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-02-17 DOI: 10.5073/VITIS.2021.60.21-27
R. Schmitz, B. Atkinson, C. Sturrock, L. Hausmann, R. Töpfer, Katja Herzog
Plant roots are essential for water and nutrient uptake and contribute to the plants' response to environmental stress factors. As the hidden half of a plant, investigation of root systems is highly challenging, most of available methods are destructive and very labour-intensive. In this proof-of-concept study, a non-invasive X-ray micro computed tomography (X-ray µCT) method was applied to investigate the phenotypic variation of the complex three-dimensional (3D) architecture of grapevine roots as a function of genotype and soil. Woody cuttings of 'Calardis Musque', 'Villard Blanc' and V3125 ('Schiava Grossa' x 'Riesling') were cultivated in polypropylene columns filled with two different soil types, clay loam and sandy loam, for 6 weeks. Afterwards, the columns were scanned once using the technique of X-ray µCT. The received raw data were analysed for the reconstruction of 3D root system models (3D model), which display a non-destructive visualization of whole, intact root systems with a spatial resolution of 42 µm. The 3D models of all investigated plants (in total 18) were applied to quantify root system characteristics precisely by measuring adventitious root length, lateral root length, total root length, root system surface area, root system volume and root growth angles from the woody cutting relative to a horizontal axis. The results showed that: (i) early root formation and root growth differed between genotypes, especially between 'Calardis Musque' and 'Villard Blanc'; and (ii) the soil type does influence adventitious root formation of V3125, but had minor effects on 'Calardis Musque' and 'Villard Blanc'. In conclusion, this innovative, high-resolution method of X-ray µCT is suitable for high resolution phenotyping of root formation, architecture, and rooting characteristics of grapevine woody cuttings in a non-destructive manner, e.g. to investigate root response to drought stress and would provide new insights into phylloxera root infection.
植物根系对水分和养分的吸收至关重要,对植物对环境胁迫的反应也起着重要作用。作为植物隐藏的一半,对根系的研究是极具挑战性的,大多数现有的方法都是破坏性的,而且非常劳动密集型。在这项概念验证研究中,采用非侵入性x射线微计算机断层扫描(x射线µCT)方法研究了葡萄根复杂三维(3D)结构的表型变化作为基因型和土壤的函数。“Calardis Musque”、“Villard Blanc”和V3125(“Schiava Grossa”和“Riesling”)的木本扦插在聚丙烯柱中,用两种不同的土壤类型(粘土壤土和砂壤土)种植6周。之后,使用x射线微CT技术对柱进行一次扫描。对接收到的原始数据进行分析,重建三维根系模型(3D模型),以42µm的空间分辨率显示完整、完整的根系的非破坏性可视化。利用三维模型对18株被调查植物的根系特征进行定量分析,通过测量木材砍伐过程中相对于水平轴的不定根长度、侧根长度、总根长度、根系表面积、根系体积和根系生长角度来精确量化根系特征。结果表明:(1)不同基因型间根的早期形成和根的生长存在差异,特别是‘卡拉迪斯Musque’和‘维拉德Blanc’;(ii)土壤类型对V3125不定根的形成有影响,但对'Calardis Musque'和'Villard Blanc'的影响较小。综上所述,这种创新的高分辨率x射线微CT技术适用于葡萄扦插根形成、结构和根系特征的非破坏性高分辨率表型分析,例如研究根系对干旱胁迫的反应,并为根瘤蚜根系侵染提供新的见解。
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引用次数: 2
Guiding principles for identification, evaluation and conservation of Vitis vinifera L. subsp. sylvestris 葡萄亚种鉴定、评价和保护的指导原则。钾盐
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2017-07-13 DOI: 10.5073/VITIS.2017.56.127-131
G. Zdunić, E. Maul, J. E. Dias, G. M. Organero, F. Carka, E. Maletić, S. Savvides, G. Jahnke, Z. Nagy, D. Nikolic, D. Ivanišević, K. Beleski, V. Maras, Milena Mugosa, V. Kodžulović, T. Radić, K. Hančević, A. Mucalo, Katarina Lukšić, L. Butorac, L. Maggioni, A. Schneider, T. Schreiber, T. Lacombe
Conservation of grapevine genetic resources is an important and long lasting task. Here, partners of the InWiGrape Activity of the European Cooperative Programme for Plant Genetic Resources have proposed a set of descriptors that will assist in identification, conservation and study of genetic resources of Vitis vinifera L. subsp. sylvestris. A distribution map of Vitis vinifera L. subsp. sylvestris populations in Europe was produced, with on-line access through the European Vitis Database. The several different aspects of conservation of Vitis vinifera L. subsp. sylvestris including bibliographical references, identification in the wild, in situ and ex situ conservation have been discussed. The descriptors and the map will assist different stakeholders, working on biodiversity and ecosystems in more effective conservation of wild grapevine genetic resources.
葡萄遗传资源的保护是一项重要而持久的任务。在此,欧洲植物遗传资源合作计划InWiGrape活动的合作伙伴提出了一套描述符,将有助于葡萄(Vitis vinifera L. subsp)遗传资源的鉴定、保护和研究。结果表明。葡萄属植物的分布图。制作了欧洲葡萄树种群,并可通过欧洲葡萄树数据库联机获取。葡萄属植物保护的几个不同方面。从文献参考、野外鉴定、就地保护和迁地保护等方面进行了讨论。描述符和地图将帮助致力于生物多样性和生态系统的不同利益攸关方更有效地保护野生葡萄藤遗传资源。
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引用次数: 7
The European "Vitis" Database (www.eu-vitis.de)- a technical innovation through an online uploading and interactive modification system 欧洲“Vitis”数据库(www.eu-vitis.de)-通过在线上传和互动修改系统的技术创新
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2012-01-01 DOI: 10.5073/VITIS.2012.51.79-85
E. Maul, K. Sudharma, S. Kecke, G. Marx, C. Müller, L. Audeguin, M. Boselli, J. Boursiquot, B. Bucchetti, F. Cabello, R. Carraro, M. Crespan, M. T. Andrés, J. E. Dias, Jana Ekhvaia, L. Gaforio, M. Gardiman, S. Grando, D. Argyropoulos, O. Jandurová, E. Kiss, J. K. Kontić, P. Kozma, T. Lacombe, V. Laucou, D. Legrand, D. Maghradze, D. Marinoni, E. Maletić, F. Moreira, G. Muñoz-Organero, G. Nakhutsrishvili, I. Pejić, E. Peterlunger, D. Pitsoli, D. Pespisilova, D. Preiner, S. Raimondi, F. Regner, G. Savin, S. Savvides, A. Schneider, C. Sereno, S. Šimon, M. Staraz, L. Zulini, R. Bacilieri, P. This
The objective of the European Vitis Database is to safeguard and enhance germplasm by monitoring its preservation. Two issues are strongly related to that purpose: (1) participation of collections covering almost all grape biodiversity and (2) assessment of accessions trueness to type. In the scope of the European project GrapeGen06 efforts have been made towards both objectives. The 35 participating grape germplasm repositories are found between the Iberian Peninsula and Transcaucasia, thus covering a broad range of grape diversity. Altogether they maintain 32,410 accessions. However with respect to biodiversity, gaps are still evident and further collections need to be included and trueness to type assessment absolutely needs to be pursued to organize duplication of endangered genotypes. Within the GrapeGen06 project focus was laid on the establishment of a database conferring the collection holders a high degree of responsibility and independence. Hence for the first time in a European Central Crop Database an on-line uploading application and an interactive modification system for data administration was implemented. These innovations disburden the database manager and offer the curators of collections more flexibility. Prerequisites for data import, descriptors applied, access levels, database contents, uploading, export and search functions are described.
欧洲葡萄数据库的目标是通过监测其保存情况来保护和提高种质资源。有两个问题与这一目的密切相关:(1)参与几乎涵盖所有葡萄生物多样性的收藏;(2)评估资料的类型真实性。在欧洲项目GrapeGen06的范围内,已经为实现这两个目标做出了努力。35个参与的葡萄种质资源库位于伊比利亚半岛和外高加索之间,因此涵盖了广泛的葡萄多样性。它们总共有32,410个词条。然而,就生物多样性而言,差距仍然很明显,需要进一步收集,并且绝对需要追求类型评估的真实性,以组织濒危基因型的复制。在GrapeGen06项目中,重点是建立一个数据库,赋予集合所有者高度的责任和独立性。因此,首次在欧洲中央作物数据库中实施了联机上传应用程序和数据管理的交互式修改系统。这些创新减轻了数据库管理员的负担,并为集合管理员提供了更大的灵活性。介绍数据导入的前提条件、使用的描述符、访问级别、数据库内容、上传、导出和查询功能。
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引用次数: 75
Anthesis, pollination and fruitset in Pinot Noir 黑皮诺的花期、授粉和结果
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2004-07-28 DOI: 10.5073/VITIS.2004.43.65-68
J. Heazlewood, S. Wilson
Aspects of pollination and resulting fruitset in Vitis vinifera cv. Pinot Noir were investigated in a cool climate wine area of Southern Tasmania (Australia). Changes in the appearance of the stigmatic surface and pollen grains were recorded using environmental scanning electron microscopy (ESEM). Flowers with the calyptra intact (before capfall), showed an apparently turgid stigmatic surface and pollen grains present on the surface were slender and elongated (L/D 35 μm/15 μm). Stigmas had a more flaccid appearance after capfall and pollen grains were more spherical and less elongated in shape (L/D 28 μm/20 μm). Pollen was visible on the stigma surface before capfall, indicating that anthesis occurred whilst the calyptra was in place. Pollen viability tests showed that the pollen was already viable at this stage, and it remained viable until after the flower had been open for several days. Fluorescence micrographs showed no evidence of pollen tube growth until after capfall. Flowers covered by waxed paper bags to eliminate external pollen and thus allow only self-pollination, gave a percentage fruitset equal to that of flowers where external pollen was not excluded. Results indicate that Pinot Noir can be self-pollinated, and that while anthesis commences prior to capfall, fertilisation does not proceed until after capfall.
葡萄传粉及结果方面的研究。黑皮诺在南塔斯马尼亚(澳大利亚)一个气候凉爽的葡萄酒产区进行了研究。利用环境扫描电镜(ESEM)记录了柱头表面和花粉粒外观的变化。花萼完整时(柱头脱落前),柱头表面明显肿胀,花粉粒细长、伸长(L/D 35 μm/15 μm)。柱头脱落后柱头呈现较弱的形态,花粉粒呈球形而非细长状(L/D 28 μm/20 μm)。柱头脱落前柱头表面可见花粉,表明花期发生在萼盖就位时。花粉活力试验表明,花粉在这一阶段已经具有活力,并且在花开放几天后仍具有活力。荧光显微照片显示花粉管生长的证据,直到帽落后。用蜡纸袋包住的花,以消除外部花粉,从而只允许自花授粉,结果的百分比等于没有排除外部花粉的花的百分比。结果表明,黑皮诺可以自花授粉,虽然开花在采穗前就开始了,但直到采穗后才开始受精。
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引用次数: 23
Yield, fruit quality, bud fertility and starch reserves of the wood as a function of leaf removal in Vitis vinifera - evidence of compensation and stress recovering. 葡萄叶片去除对木材产量、果实品质、芽育性和淀粉储量的影响——补偿和胁迫恢复的证据。
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 1990-01-01 DOI: 10.5073/VITIS.1990.29.199-221
M. C. Candolfi-Vasconcelos, W. Koblet
Einflus unterschiedlicher Entblatterung auf Ertrag, Traubenqualitat, Knospenfruchtbarkeit und Starkegehalt des Holzes von Vitis vinifera sowie Kompensations- und Erholungsvermogen An Ertragsreben wurde der Einflus des Entfernens von Hauptblattern oder Geiztrieben auf Gesamtblattflache, Traubenertrag und -qualitat sowie Starkegehalt des Holzes studiert. Die Bedeutung von Haupt- und Geizblattern sowie des Zeitpunktes der Entblatterung auf dss Verrieseln wurde untersucht. Ziel der Studie war, Kompensationsmechanismen und -limiten der Rebe unter Stresbedingungen kennenzulernen, wie sie durch eine Entblatterung verursacht werden. Ferner sollte die Erholung der Pflanze nach langerer Streseinwirkung erforscht werden. Wurden die Hauptblatter entfernt (L = nur Geizblatter}, so bildeten die Pflanzen mehr Geiztriebe mit einer groseren Anzahl Blatter. Dies fuhrte nach dem ersten Stresjahr zu einer ungefahr gleichen Gesamtblattflache wie bei den Kontrollpflanzen. Nach einem weiteren Stresjahr jedoch hatten die L-Pflanzen zwar weiterhin mehr Geizblatter, allerdings von geringerer Grose. Hieraus resultierte eine im Vergleich zur Kontrolle verringerte Gesamtblattflache. Pflanzen nur mit Hauptblattern (M) kompensierten das Fehlen der Geiztriebe mit verzogerter Blattalterung und spaterem Blattfall. Auch hier ergab sich nach dem zweiten Stresjahr eine geringere Blattgrose. Der Traubenertrag der L-Pflanzen wurde im 1. Jahr durch die Blattentfernung kaum negativ beeinflust, aber im 2. Jahr war er 50 % niedriger als in der Kontrolle. Bei den M-Pflanzen ergab sich in beiden Jahren kein verringerter Ertrag. Der Zuckergehalt der Trauben war in den L-Pflanzen im 1. Stresjahr leicht erhoht, nicht aber im 2. Jahr. Diese Reben hatten wahrend beider Jahre eine bessere Beerenfarbe. Die Blattflache vom Zeitpunkt der Blute bis 2-3 Wochen danach ist fur den Traubenertrag entscheidend. Eine Entblatterung zu diesem Zeitpunkt verursachte nicht nur ein Verrieseln, sondern im folgenden Jahr zusatzlich eine reduzierte Knospenfruchtbarkeit. Die Zuckereinlagerung in den Trauben hangt von der assimilierenden Blattflache wahrend der Reifeperiode ab. Der Starkegehalt im Holz war nach 2 Stresjahren erheblich reduziert. Es ergaben sich schwach positive Korrelationen zwischen Zuckergehalt des Mostes und Starkegehalt des Holzes. Die Zuckereinlagerung in die Traube und das Auffullen der Starkereserven im Holz ging bereits in der auf eine Stresbehandlung folgenden Saison normal vonstatten. Ein normaler Ertrag war l Jahr nach einem langeren Entblatterungsstres jedoch noch nicht moglich, da die Bildung der Infloreszenzen bekanntlich wahrend dieser Zeit (in unserem Fall die Stresperiode) einsetzt. Erst im 2. Jahr kam es zu einer vollstandigen Erholung der Pflanze.
许多差异的差异,包括果实、葡萄肥、蒜头肥胖症、肥胖症、盈利葡萄树的高脂肪、补偿和康复脂肪。研究了旺角、秃驴的看法,以及庆生时的看法。这项研究的目的是,了解在压力状态下的补偿机制,以及葡萄藤在紧张状态下可能造成的损坏。此外,科学家要研究这种植物延展的功效。在这样的案例中,花瓣被剔除(L =突然吝啬),则植物结出更多的吝啬,有更平稳的减少的叶。这样的做法在第一年后即会造成与控制科相同的冠状菌。不过,心理压力后后的一年,这种树虽然保存较低,但保存的时间却较短。都因为这导致了导管失灵只有用大叶(M)取代敏锐的嫩芽,这种嫩叶会导致叶老化和单调的衰竭。目前的大压力状态后,回归冠状动脉一年级的葡萄收成他们没对你的蚜虫影响大,但细小的果实…就更小了百分之五十在过去两年间,m作物的产量没有减少。我在研究通常意外的时间,但意外的时间.年两年的葡萄收成都比以前好22周后就是葡萄收成的关键。当时的瘟疫不但造成了肿块,而且还在次年造成了肥胖症。葡萄中的糖分会随着冠状菌的逐渐衰竭而增加,因此在很大的压力下,木头的浓度意外降低。结果显示浆果的糖分和木质粗味之间的关系并不显著。可见葡萄上的糖分,以及红杉上的贵宾,已经是接下来压力下治疗后的正常季节。但是,如果它捶得更久,那么普通的产量就无法得到。因为外来破坏的破坏是众所周知的,可以在整个时间(就我们的例子而言)产生的。两个月?那年,这种植物得到了充分的休养。
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引用次数: 183
Possible integrated control of grape-vine sour-rot 葡萄酸腐病可能的综合防治
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 1986-01-01 DOI: 10.1201/9781003211426-35
M. Bisiach, G. Minervini, F. Zerbetto
Etiopathogenie de ce type de maladie. Les principaux agents pathogenes sont des levures, Kloeckera apiculata, Saccharomycopsis vini, Hanseniaspora uvarum et autres dont les vecteurs sont des insectes comme Drosophila. On propose l'utilisation de filets en plastique ou en tissu pour eviter l'infestation par les insectes et l'utilisation de clones dont les fruits ont une peau epaisse
这类疾病的病因。主要的病原体是酵母,蜜蜂Kloeckera apiculata, Saccharomycopsis vini, Hanseniaspora uvarum和其他昆虫,如果蝇。建议使用塑料或织物网来防止昆虫侵扰,并使用果实有厚皮的无性系
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引用次数: 31
Virus diseases of the grapevine in a Sicilian herbarium of the past century 上个世纪西西里植物标本室的葡萄藤病毒病
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 1975-01-01 DOI: 10.5073/VITIS.1974.13.329-335
G. Martelli, G. Piro
Dried grapevine specimens, collected in Sicily between 1880 and 1886, and showing different morphological and chromatic modifications, were found in a herbarium established in 1830 by Dr. FRANCESCO MINA PALUMBO, a physician of Castelbuono (Palermo). On the basis of symptoms still visible on the specimens and of the herbarium's original explanatory notes, the diseased material was identified as being affected by fanleaf, yellow mosaic and leafroll. Rebvirosen in einem sizilianischen Herbarium aus dem letzten Jahrhundert Getrocknete Exemplare von Reben, die zwischen 1880 und 1886 in Sizilien fur ein von Dr. FRANCESCO MINA PALUMBO, einem Arzt aus Castelbuono (Palermo), begrundetes Herbarium gesammelt worden waren, zeigten verschiedene morphologische und farbliche Modifikationen. Die noch sichtbaren Symptome an den Herbarexemplaren erlauben zusammen mit den erlauternden Originalnotizen eine Diagnose auf Befall durch Reisigkrankheit, Gelbmosaik und Blattrollkrankheit.
1880年和1886年斯克拉福斯培养基大学,以及不同时期的形态和肤色,在1830年生于佛朗西斯科·米娜·帕伦博斯博士和对立分子帕格诺(布宜诺斯艾利斯)的一名物理学家中长大。在这种症状的基础上仍然存在于物种和鲱鱼原始的来源,表明了声音的来源从1880年到1886年,在西西里一种西西里豪饮的葡萄,是为塞内格尔·布诺(巴勒莫)的医生,弗朗西斯科·米娜·帕伦博(FRANCESCO MINA)收集的一种葡萄酒而采集的,性情和颜色都发生了不同的形态形态。愉快的贝壳表面仍然存在明显的症状,加上最初的说明,能被诊断出出现了大米病、黄疸病和风花病。
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引用次数: 4
Ovule development in seeded and seedless grapes 有籽和无籽葡萄的胚珠发育
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 1970-01-01 DOI: 10.5073/VITIS.1970.9.7-14
B. Barritt
Female gametophyte and early embryo and endosperm development were examined in 3 seedless grape varieties, Himrod, Interlaken Seedless and N. Y. 15302, and in their seeded parent Ontario. A high proportion of functional embryo sacs was found at full bloom in all varieties. Fertilization occurred in all varieties. Precocious initiation of endosperm was observed with Himrod and extremely rapid development of endosperm was found with N. Y. 15302. Early degeneration of zygotes, failure of the zygotes to divide, and endosperm degeneration were observed in the seedless varieties with smallest seed remnants, Himrod and Interlaken Seedless. Imperfect development of the zygote and endosperm resulted in seed abortion (stenospermy) and seedless fruit (stenospermocarpy) in Himrod and Interlaken Seedless varieties.
对3个无核葡萄品种Himrod、Interlaken无核葡萄和ny 15302及其种子亲本Ontario的雌性配子体和早期胚、胚乳发育进行了研究。在所有品种中,开花时功能胚囊的比例都很高。所有品种均发生受精。用Himrod观察到胚乳早熟,用N. Y. 15302观察到胚乳发育极快。无籽品种希姆罗德(Himrod)和因特拉肯(Interlaken)无籽品种的受精卵早期退化,受精卵分裂失败,胚乳退化。由于合子和胚乳发育不全,导致了希姆罗德和因特拉肯无籽品种的种子败育(窄精子)和无籽结实(窄精子)。
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引用次数: 27
The effect of leaf area and crop level on the concentration of amino acids and total nitrogen in 'Thompson Seedless' grapes 叶面积和作物水平对汤普森无籽葡萄氨基酸和总氮浓度的影响
IF 1.1 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 1970-01-01 DOI: 10.5073/VITIS.1970.9.196-206
W. Kliewer, C. Ough
20 to 50% of the leaves and 25 to 75% of the clusters on 'Thompson Seedless' vines were removed 12 to 16 days after anthesis. In addition, 25 and 50% of the   leaves on other vines were removed 35 and 58 days after anthesis, with no cluster thinning. Total soluble solids in fruits from vines with less than 5 cm 2 of leaf surface per gram of fruits was markedly reduced, while concentration in fruits from vines with a ratio above 10 was considerably higher, compared to the concentration in fruits from vines with ratios intermediate between these two values. The concentrations of arginine, proline, total free amino acids, and total N in berry juice were greatly increased by reducing the crop load. Also, the concentration of each of these substances increased during fruit ripening. A significant correlation between leaf area per vine and level of arginine in the juice was obtained. The concentration of proline in the juice was highly correlated with fruit maturity (°B) and with leaf area per unit weight of fruits. Total nitrogen was also correlated with leaf area per unit weight of fruits. Between 4 and 12 cm 2 leaf area per gram of fruits there was a linear increase in the concentration of proline and total nitrogen in berry juice, while above 12 cm 2 there was generally little further increase in the level of these substances. The ratio of arginine to proline decreased with fruit maturity and with smaller crop weights per vine. Total free amino acids accounted for 64 to 75% of the total nitrogen in the juice of grapes during the fruit-ripening period. Defoliation within 16 days after anthesis reduced crop yields, while later defoliations did not significantly reduce yields.
“汤普森无籽”藤上20%至50%的叶子和25%至75%的簇在开花后12至16天被移除。此外,在其他藤上,25%和50%的叶子在开花后35天和58天被去除,没有簇变薄。每克果实叶面小于5 cm 2的葡萄藤果实中可溶性固形物的含量显著降低,而比值大于10的葡萄藤果实中的可溶性固形物含量明显高于比值介于这两个值之间的葡萄藤果实中的可溶性固形物含量。减少作物负荷显著提高了浆果汁中精氨酸、脯氨酸、总游离氨基酸和总氮的含量。此外,这些物质的浓度在果实成熟过程中都有所增加。单株叶面积与果汁中精氨酸含量呈显著相关。果汁中脯氨酸浓度与果实成熟度(°B)和果实单位重量叶面积高度相关。全氮也与果实单位重量叶面积相关。在每克果实叶面积为4 ~ 12平方厘米之间,浆果汁中脯氨酸和总氮的浓度呈线性增加,而在12平方厘米以上,这些物质的含量一般没有进一步增加。精氨酸与脯氨酸的比值随着果实的成熟和单株产量的减少而降低。在果实成熟期,游离氨基酸占葡萄汁总氮的64 ~ 75%。开花后16天内的落叶会降低作物产量,而较晚的落叶则不会显著降低产量。
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引用次数: 35
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Vitis
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