Transfusion and Apheresis Science最新文献_第6页

Genetic determinants of plasma testosterone in male blood donors are associated with altered red blood cell characteristics and survival in cold storage and after transfusion 男性献血者血浆睾酮的遗传决定因素与红细胞特性的改变以及冷藏和输血后的存活率有关。

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-10-15 DOI: 10.1016/j.transci.2024.104017

Fang Fang , Nareg H. Roubinian , Scott-Wesley Bean , Cassie Kemmler , Grier G. Page , Tamir Kanias

Genetic mutations in genes regulating plasma testosterone in men may interfere with effective erythropoiesis, and may result in red blood cell (RBC) dysfunction and hemolysis. The aim of this study was to identify genetic polymorphisms in male donors that regulate plasma testosterone and impact RBC survival in cold storage and after transfusion. We evaluated nine single nucleotide polymorphisms (SNPs) previously reported to be associated with circulating testosterone in male plasma. These SNPs were linked with donor-component-recipient databases (NIH REDS program) to determine SNP associations with donor RBC hematological indices, osmotic and oxidative hemolysis, and RBC transfusion effectiveness defined as adjusted hemoglobin increments (delta hemoglobin, ΔHb) following a single RBC unit transfusion. Four of the nine testosterone SNPs were located on the X chromosome, of which two (rs7057002, rs73629199) were significantly associated with reduced hemoglobin increments (0.2 and 0.3 g/dL, respectively) compared with reference alleles in transfused recipients. Seven of the nine testosterone SNPs were associated with significant changes in RBC susceptibility to osmotic hemolysis including a missense mutation in the major plasma carrier of testosterone (SHBG, rs6259), and four SNPs with changes in oxidative hemolysis. Four SNPs were associated with decreased RBC count, hemoglobin, and hematocrit. Ancestry/ethnicity-specific (African and Hispanic) associations were observed between two SNPs (rs7057002, rs7879462) and oxidative hemolysis. Genetic determinants of plasma testosterone in male donors significantly impact the quality and transfusion effectiveness of cold stored RBCs. Testosterone SNPs associated with decreased RBC transfusion effectiveness may have clinical implications and warrant further revaluation.

男性血浆睾酮调节基因的基因突变可能会干扰有效的红细胞生成，并可能导致红细胞（RBC）功能障碍和溶血。本研究旨在确定男性捐献者中调节血浆睾酮并影响红细胞冷藏存活率和输血后存活率的基因多态性。我们评估了之前报道的与男性血浆中循环睾酮相关的九个单核苷酸多态性（SNPs）。这些 SNP 与供体-成分-受体数据库（美国国立卫生研究院 REDS 计划）相连接，以确定 SNP 与供体 RBC 血液指数、渗透性溶血和氧化性溶血以及 RBC 输血效果（定义为单次 RBC 单位输血后的调整血红蛋白增量（δ血红蛋白，ΔHb））的关系。九个睾酮 SNPs 中有四个位于 X 染色体上，其中两个（rs7057002 和 rs73629199）与输血受体的参考等位基因相比，与血红蛋白增量的降低（分别为 0.2 和 0.3 g/dL）显著相关。在 9 个睾酮 SNPs 中，有 7 个与红细胞对渗透性溶血敏感性的显著变化有关，包括睾酮的主要血浆载体（SHBG，rs6259）中的一个错义突变，以及与氧化性溶血变化有关的 4 个 SNPs。四个 SNP 与红细胞计数、血红蛋白和血细胞比容下降有关。在两个 SNPs（rs7057002、rs7879462）与氧化溶血之间观察到了祖先/种族特异性（非洲裔和西班牙裔）关联。男性捐献者血浆睾酮的遗传决定因素对冷藏红细胞的质量和输血效果有显著影响。与红细胞输血效果下降相关的睾酮 SNPs 可能会对临床产生影响，值得进一步评估。

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引用次数: 0

Donor clinical characteristics and impacts on transfusion recipient outcomes 捐献者的临床特征及其对输血受体结果的影响。

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-10-15 DOI: 10.1016/j.transci.2024.104012

Shuoyan Ning , Michelle Zeller , Nancy M. Heddle

Clinical characteristics of blood donors may affect short- and long-term outcomes of transfusion recipients. The impact of donor sex and age on recipient outcomes have not yielded consistent results in observational studies. One recently published randomized controlled trial (iTADS) addressing the impact of donor sex on recipient outcomes noted no differences between a female versus male transfusion strategy; a second Canadian multicenter trial has just been funded. Other donor characteristics - including pregnancy history, smoking status, obesity, and chronic illnesses - remain incompletely explored. More robust clinical studies with vein-to-vein capabilities are needed to understand the complex interplay between donors and recipients.

献血者的临床特征可能会影响输血受者的短期和长期治疗效果。关于献血者性别和年龄对受血者预后的影响，观察性研究的结果并不一致。最近发表的一项随机对照试验（iTADS）探讨了献血者性别对受血者预后的影响，发现女性与男性输血策略之间没有差异；第二项加拿大多中心试验刚刚获得资助。其他捐献者特征--包括怀孕史、吸烟状况、肥胖和慢性疾病--仍未得到充分探讨。要了解供体和受体之间复杂的相互作用，还需要更多具有静脉对静脉功能的临床研究。

引用次数: 0

Sex discrepancies in blood donation: Implications for red blood cell characteristics and transfusion efficacy 献血中的性别差异：对红细胞特性和输血效果的影响

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-10-15 DOI: 10.1016/j.transci.2024.104016

Abdulrahman Alshalani , Hamood AlSudais , Sarah Binhassan , Nicole P. Juffermans

Red blood cell (RBC) transfusions carry risks, and the mechanisms mediating adverse transfusion outcomes are not fully understood. This review explores the impact of donor sex and donor-recipient sex mismatch on RBC characteristics and transfusion outcomes. Females, at least those in their reproductive age, have a higher proportion of young RBCs in the circulation when compared to males, associated with higher post transfusion recovery. Also, female RBCs exhibit a greater resilience to the storage lesion, with lower hemolysis rates and better rheologic properties. Despite these qualities, transfusion of female RBCs may be associated with adverse transfusion outcomes, such as pulmonary injury, increased mortality, and immunomodulatory effects, which may differ depending on the sex of the recipient, although not all observations are consistent. As a potential mechanism, the presence of immature RBCs, especially reticulocytes, in transfused blood is associated with immunomodulatory effects. Reticulocytes contain residual cellular components which can interact with surrounding blood cells and endothelial cells, in particular in neonates and cancer patients. Understanding the influence of donor sex and RBC age-subpopulation on RBC quality, and investigating the risks and benefits of immature RBCs in transfusions, offers opportunities for optimizing transfusion practices.

输注红细胞（RBC）存在风险，而导致不良输血结果的机制尚未完全明了。本综述探讨了献血者性别和献血者与受血者性别不匹配对红细胞特性和输血结果的影响。与男性相比，女性（至少是处于生育年龄的女性）血液循环中年轻 RBC 的比例较高，这与较高的输血后恢复率有关。此外，女性红细胞对储存病变有更强的抵抗力，溶血率更低，流变学特性更好。尽管雌性红细胞具有这些特性，但输注雌性红细胞可能会导致不良输血结果，如肺部损伤、死亡率升高和免疫调节作用，这可能因受血者的性别而异，但并非所有观察结果都一致。作为一种潜在的机制，输血血液中存在未成熟的红细胞，尤其是网织红细胞，与免疫调节作用有关。网织红细胞含有残留的细胞成分，可与周围的血细胞和内皮细胞相互作用，尤其是在新生儿和癌症患者中。了解捐献者性别和网织红细胞年龄亚群对网织红细胞质量的影响，并调查输血中未成熟网织红细胞的风险和益处，为优化输血操作提供了机会。

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引用次数: 0

RBC subpopulations in RCCs affected by donor factors 受供体因素影响的 RCC 中的红细胞亚群。

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-10-15 DOI: 10.1016/j.transci.2024.104010

Mahsa Yazdanbakhsh , Celina Phan , Nishaka William , Jason P. Acker

Understanding red blood cell (RBC) subpopulations is crucial for comprehending donor variability and enhancing transfusion outcomes. This review highlights the significance of RBC subpopulations, focusing on the properties of biologically young and old RBCs and underscores how donor variability impacts transfusion outcomes. The role of senescent RBCs in adverse transfusion reactions and the emerging significance of circulating erythroid cells (CECs) is discussed. RBC aging and the role of oxidative stress and aging mechanisms is highlighted. Changes in RBC flexibility, calcium homeostasis, band 3 protein modifications, membrane microvesiculation, 2,3-diphosphoglycerate (2,3-DPG) levels, and immunological markers like CD47 and CD55 contribute to RBC clearance and erythrophagocytosis. Also, methods of characterizing / separating of biologically young and old RBC subpopulations is introduced. This review emphasizes the importance of RBC subpopulations in understanding donor variability and improving transfusion outcomes.

了解红细胞（RBC）亚群对于理解供体变异和提高输血效果至关重要。本综述强调了红细胞亚群的重要意义，重点介绍了生物学上年轻和衰老红细胞的特性，并强调了供体变异如何影响输血结果。文章还讨论了衰老 RBC 在输血不良反应中的作用以及循环红细胞 (CEC) 的新意义。重点介绍了红细胞的衰老以及氧化应激和衰老机制的作用。RBC 弹性、钙稳态、带 3 蛋白修饰、膜微vesiculation、2,3-二磷酸甘油酯（2,3-DPG）水平以及 CD47 和 CD55 等免疫标记物的变化有助于 RBC 的清除和红细胞吞噬。此外，还介绍了表征/分离生物学上年轻和年老的 RBC 亚群的方法。本综述强调了 RBC 亚群在了解供体变异性和改善输血结果方面的重要性。

引用次数: 0

Circulating microRNAs and migrasomes: Present and future bullseyes in extracorporeal photopheresis? 循环微RNA和迁移体：体外光子疗法的现状与未来？

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-09-26 DOI: 10.1016/j.transci.2024.104008

Yandy Marx Castillo-Aleman

In the realms of extracorporeal photopheresis (ECP), only a few studies have reported the usefulness of circulating microRNAs as predictors of responses. This letter also highlights the putative role of novel organelles termed “migrasomes” in the ECP-triggered immunological responses.

在体外光子穿刺（ECP）领域，只有少数研究报告了循环微RNA作为反应预测因子的作用。这封信还强调了被称为 "migrasomes "的新型细胞器在 ECP 触发的免疫反应中可能发挥的作用。

引用次数: 0

Effect of temporary storage of cryopreserved cellular therapy products at −80⁰ celsius on cell recovery and viability 在-80⁰摄氏度下暂时储存低温保存的细胞治疗产品对细胞恢复和活力的影响

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-09-24 DOI: 10.1016/j.transci.2024.104007

Tobias Cohen , Yvette C. Tanhehco

Cellular therapy (CT) involving the transplantation of hematopoietic progenitor cells (HPC) is a treatment modality for both benign and malignant disorders. All autologous products require cryopreservation while allogeneic product cryopreservation became more common during the Coronavirus disease 2019 pandemic. Cells are stored in liquid nitrogen (LN₂) freezers which can malfunction and products may have to be temporarily stored in a mechanical −80 °C freezer if additional LN₂ freezer space is not available. The practice of temporary short-term −80 °C storage is present but there is no study to show that the product is unaffected by the temporary storage at a significantly warmer temperature. In this study, we identified previously collected CT products that were cryopreserved for now-deceased recipients that had remaining cryovials with aliquots of products for quality control purposes. Vials from 20 collections were split into 4 groups of 5 in with one vial placed in temporary storage at −80 °C for 2–5 weeks before returning to LN₂ storage while another vial remained in LN₂ storage for the entire duration of the study. The vials were then simultaneously thawed, processed, and evaluated for total nucleated cell (TNC) and CD34 + cell count and TNC and CD34 + cell viability to determine if there were any differences induced by temporary −80 °C storage. No statistically significant differences were seen after 4 weeks of −80 °C storage; however, after 5 weeks, a statistically significant decrease in TNC viability and viable TNC count, but not CD34 + cell viability and viable CD34 + cell count was observed. These results provide some reassurance to CT processing labs that if there is a failure in their LN₂ storage for cryopreserved products, these products may be safely stored at −80 °C for up to 4 weeks and returned to LN₂ storage without compromising CD34 + cell viability.

细胞疗法（CT）涉及造血祖细胞（HPC）的移植，是良性和恶性疾病的一种治疗方式。所有自体产品都需要低温保存，而异体产品的低温保存在 2019 年冠状病毒疾病大流行期间变得更加普遍。细胞储存在液氮（LN2）冷冻箱中，而液氮冷冻箱可能会出现故障，如果没有额外的液氮冷冻空间，产品可能不得不暂时储存在机械式-80 °C冷冻箱中。目前已有-80 °C短期临时储存的做法，但没有研究表明产品在温度明显更高的环境中临时储存不会受到影响。在这项研究中，我们确定了以前为现已去世的受者冷冻保存的 CT 产品，这些产品的剩余冷冻瓶中有用于质量控制的等分产品。我们将 20 个收集的样品瓶分成 4 组，每组 5 个，其中一个样品瓶在 -80 °C 下暂时保存 2-5 周，然后再返回 LN2 保存，而另一个样品瓶在整个研究期间都保持在 LN2 保存状态。然后同时解冻、处理和评估小瓶中的总核细胞（TNC）和 CD34 + 细胞计数以及 TNC 和 CD34 + 细胞存活率，以确定-80 °C临时储存是否会引起任何差异。在-80 °C储存4周后，未发现有统计学意义的差异；但在5周后，观察到TNC存活率和存活的TNC计数有统计学意义的下降，而CD34 +细胞存活率和存活的CD34 +细胞计数没有下降。这些结果为 CT 处理实验室提供了一些保证，即如果冷冻保存产品的 LN2 储存发生故障，这些产品可以安全地在 -80 °C 下储存长达 4 周，然后再返回 LN2 储存，而不会影响 CD34 + 细胞的存活率。

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引用次数: 0

Evaluation of riboflavin concentrations and light intensities on bacteria reduction in platelets using visible light 利用可见光评估核黄素浓度和光强度对血小板中细菌减少的影响。

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-09-18 DOI: 10.1016/j.transci.2024.104006

Hong Liu, Qin Mo, Jianhao Yang, Yao Jia, Rongna Ma, Xiaofei Wu, Yuwen Huang, Xun Wang

Bacterial contamination in platelets has been a major concern over the years. In this study, we showed that treatment with 420 nm visible light with various concentrations of riboflavin in platelets reduced E. coli and S. aureus by 0–1.56 and 0.3–2.02 logs (50 mW/cm²), 2.24–4.77 and 0.73–3.26 logs (75 mW/cm²), and ≥ 5.14 and ≥ 5.27 logs (100 mW/cm²). Treatment with high-intensity light (100 mW/cm²) and high concentrations of riboflavin (400 µM and 500 µM) effectively reduced both bacteria in platelets by over 4 logs. The study also found a positive correlation between bacterial reduction and light intensity, as well as riboflavin concentration in a dose-dependent manner. These results demonstrate the potential of using riboflavin and visible light to reduce the risk of bacterial contamination in platelets, and support the need for further exploration of pathogen reduction using 420 nm visible light and riboflavin.

多年来，血小板中的细菌污染问题一直备受关注。在这项研究中，我们发现用不同浓度核黄素的 420 纳米可见光处理血小板后，大肠杆菌和金黄色葡萄球菌分别减少了 0-1.56 和 0.3-2.02 个对数值（50 mW/cm2）、2.24-4.77 和 0.73-3.26 个对数值（75 mW/cm2）以及≥ 5.14 和≥ 5.27 个对数值（100 mW/cm2）。使用高强度光（100 mW/cm2）和高浓度核黄素（400 µM 和 500 µM）可有效减少血小板中的两种细菌，减少幅度超过 4 个对数值。研究还发现，细菌减少量与光照强度以及核黄素浓度呈正相关，且呈剂量依赖性。这些结果证明了利用核黄素和可见光降低血小板中细菌污染风险的潜力，并支持进一步探索利用 420 纳米可见光和核黄素减少病原体的必要性。

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引用次数: 0

Enhancing clinical insight: Implementing validated questionnaires for comprehensive assessment of clinician expertise in transfusion medicine practices 提高临床洞察力：采用有效问卷全面评估临床医生在输血医学实践中的专业知识。

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-09-16 DOI: 10.1016/j.transci.2024.104005

Anubhav Gupta , Hari Krishan Dhawan , Romesh Jain , Ratti Ram Sharma , Vipin Kaushal , Amarjeet Singh , Neelam Marwaha

Background

Blood transfusion is a cornerstone of modern healthcare, pivotal in saving countless lives annually. However, inadequate knowledge among healthcare providers can lead to serious complications. Despite the availability of assessment tools like the Biomedical Excellence for Safer Transfusion (BEST) test, there is a need for indigenous-validated questionnaires to address knowledge gaps effectively. This study aimed to evaluate bedside transfusion medicine knowledge among clinical residents using a validated questionnaire, focusing on knowledge gaps.

Study design and methods

A cross-sectional study was conducted at a tertiary care referral center in Northern India. The questionnaire, developed based on national and international transfusion guidelines, was validated by an expert panel, and administered to 245 clinical residents. The questionnaire covered six domains related to transfusion medicine: blood component storage, blood bank procedures, transfusion-transmitted infections, administration of blood components, transfusion reactions, and transfusion practices.

Results

The study revealed varying levels of knowledge across specialties and residency years. Overall, residents scored 61 % in transfusion medicine knowledge, with Pediatrics residents demonstrating the highest scores. The incremental increase in knowledge from first to third-year residents underscores the value of continuous, experience-based learning throughout the residency period.

Discussion

Study highlights significant knowledge gaps in bedside transfusion practices among clinical residents, emphasizing the need for structured educational interventions. Tailored programs, integrated into undergraduate and postgraduate curricula, are essential to improve transfusion safety and patient outcomes. Addressing these gaps can lead to better bedside transfusion practices, reducing risks and improving the quality of patient care.

背景：输血是现代医疗保健的基石，每年挽救无数人的生命。然而，医疗服务提供者对输血知识的缺乏会导致严重的并发症。尽管有生物医学卓越安全输血（BEST）测试等评估工具，但仍需要经过本土验证的问卷来有效解决知识差距问题。本研究旨在使用有效问卷评估临床住院医师的床旁输血医学知识，重点关注知识差距：在印度北部的一家三级医疗转诊中心开展了一项横断面研究。该问卷是根据国内和国际输血指南编制的，经专家小组验证，并对 245 名临床住院医师进行了问卷调查。问卷涉及输血医学的六个相关领域：血液成分储存、血库程序、输血传播感染、血液成分管理、输血反应和输血实践：研究显示，不同专业和不同住院医师年限的住院医师对输血知识的掌握程度各不相同。总体而言，住院医师在输血医学知识方面的得分率为 61%，其中儿科住院医师的得分率最高。从第一年到第三年，住院医师的知识水平逐步提高，这凸显了在整个住院医师培训期间持续进行以经验为基础的学习的价值：讨论：该研究强调了临床住院医师在床旁输血实践方面存在的巨大知识差距，强调了采取结构化教育干预措施的必要性。在本科生和研究生课程中融入量身定制的课程，对于改善输血安全和患者预后至关重要。弥补这些不足可改善床旁输血实践，降低风险并提高患者护理质量。

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引用次数: 0

Does immunohistochemical staining predict mobilization success in multiple myeloma patients? 免疫组化染色能否预测多发性骨髓瘤患者的转移成功率？

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-09-15 DOI: 10.1016/j.transci.2024.104004

Fatma Keklik Karadag , Murat Aysin , Nur Soyer , Ajda Güneş , Denis Bozer , Derya Demir , Aysenur Arslan , Fahri Sahin , Mahmut Töbü , Guray Saydam , Filiz Vural

Background

So many risk factors for mobilization failure have been described so far. We aimed to identify the risk factors and search the possible effects of bone marrow fibrosis (BMF), CD56, c-myc, and cyclinD1 expression on mobilization.

Methods

We evaluated 189 patients with MM who were admitted for stem cell mobilization before autologous stem cell transplantation (ASCT) between 2015 and June 2021. Clinical, laboratory, treatment features, and survival outcomes were compared in patients who were successfully mobilized and who were not.

Results

Mobilization failure rate was 11.1 % (21) in our study group. Male gender, mobilization with only G-CSF, history of previous ASCT, lenalidomide exposure, and 2 lines of chemotherapy before stem cell mobilization were observed more commonly in mobilization failure group. There is no relationship between mobilization failure and BMF, CD56, c-myc, and cyclin D1 expression status in patients who received either only G-CSF or G-CSF+ chemotherapy for mobilization. Overall survival (OS) was not different in groups of patients who were successfully mobilized and who were not. Neutrophil engraftment was faster in patients who were transfused > 5 × 10⁶/kg stem cells (p = 0.015). ECOG performance status (p = 0.004), c-myc expression (p = 0.005), lenalidomide therapy before mobilization (p = 0.032), and mobilization with G-CSF+chemotherapy was found to be predictive factors for OS.

Conclusion

Even though we could not find any predictive value of CD56, c-myc, and cyclin D1 expression on mobilization, c-myc was found to be associated with low OS. Further studies with large and homogenous study population would be more informative.

背景迄今为止，动员失败的风险因素有很多。我们旨在确定这些风险因素，并研究骨髓纤维化（BMF）、CD56、c-myc和cyclinD1表达对动员可能产生的影响。方法我们评估了2015年至2021年6月期间入院进行自体干细胞移植（ASCT）前干细胞动员的189名MM患者。结果在我们的研究组中，动员失败率为11.1%（21例）。在动员失败组中，男性、仅使用G-CSF动员、既往ASCT病史、来那度胺暴露和干细胞动员前接受过2次化疗的情况更为常见。在仅接受G-CSF或G-CSF+化疗动员的患者中，动员失败与BMF、CD56、c-myc和细胞周期蛋白D1的表达状态没有关系。成功动员和未成功动员患者的总生存期（OS）没有差异。输注> 5 × 106/kg干细胞的患者中性粒细胞移植速度更快（p = 0.015）。ECOG表现状态（p = 0.004）、c-myc表达（p = 0.005）、动员前来那度胺治疗（p = 0.032）和动员时使用G-CSF+化疗被认为是OS的预测因素。对大量同质研究人群的进一步研究将提供更多信息。

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引用次数: 0

Cell throughput and performance ratio as quality indicators on hematopoietic stem cell apheresis: A single-center experience 作为造血干细胞分离质量指标的细胞吞吐量和性能比：单中心经验

IF 1.4 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2024-09-10 DOI: 10.1016/j.transci.2024.104003

Yandy Marx Castillo-Aleman , Carlos Agustin Villegas-Valverde , Antonio Alfonso Bencomo-Hernandez , Yendry Ventura-Carmenate , Shinnette Lumame , Charisma Castelo , Nameer Abdul Al-Saadawi , Mohamed Ibrahim Abu-Haleeqa , Inas El-Najjar , Fatema Mohammed Al-Kaabi

Background

Benchmarking in CD34⁺ cell apheresis is crucial for optimizing resources, ensuring consistent collection performance, and ultimately, decision-making algorithms to improve donor safety. Key performance indicators such as the “performance ratio” (PR) are applied routinely in some apheresis centers, whereas this report identifies the “cell throughput” (CT) as another quality indicator in apheresis.

Material and methods

This single-center study includes retrospective data from 117 aphereses. CT and PR were calculated based on the mononuclear cell collection (MNC) or continuous mononuclear cell collection (cMNC) protocols of the Spectra Optia® apheresis system, types of venous access, transplant settings, and

mobilization regimens.

Results

CTs (× 10⁶ CD34⁺ cells/min) were found to be greater in cMNC compared to MNC protocols (1.4 vs. 1.0, p = 0.0037), in allogeneic versus autologous (1.3 vs. 1.1, p = 0.0274), and in the mobilization regimen of G-CSF alone versus the G-CSF combined (1.3 vs. 1.0, p = 0.0249). In contrast, PR (%) was only statistically significant in favor of the cMNC protocol (213.0 vs. 186.8 for MNC).

Conclusions

CT and PR are feasible quality indicators on CD34⁺ cell apheresis, are easy to calculate and implement, and have clinical and administrative implications. Analyzing CT and PR may strengthen the institutional criteria for selecting cMNC or MNC protocols; they may also be used to evaluate the performance of new personnel or cell separator devices or, eventually, trigger investigations for those aphereses under-collected by specific thresholds.

背景CD34+细胞采血中的基准指标对优化资源、确保一致的采集性能以及最终改善供者安全的决策算法至关重要。一些血液净化中心常规采用 "性能比"（PR）等关键性能指标，而本报告将 "细胞吞吐量"（CT）确定为血液净化的另一个质量指标。CT 和 PR 的计算基于 Spectra Optia® 无细胞采集系统的单核细胞采集 (MNC) 或连续单核细胞采集 (cMNC) 方案、静脉通路类型、移植设置和动员方案。结果 cMNC 与 MNC 方案相比（1.4 vs. 1.0，p = 0.0037），异体与自体相比（1.3 vs. 1.1，p = 0.0274），单用 G-CSF 与 G-CSF 联合动员方案相比（1.3 vs. 1.0，p = 0.0249），CTs（×106 CD34+ 细胞/分钟）更高。结论CT和PR是CD34+细胞分离的可行质量指标，易于计算和实施，具有临床和管理意义。分析CT和PR可加强选择cMNC或MNC方案的机构标准；它们还可用于评估新人员或细胞分离器设备的性能，或最终触发对未按特定阈值收集到的血球进行调查。

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引用次数: 0