Transfusion and Apheresis Science最新文献_第9页

Predicting intraoperative transfusion volumes of blood products in cardiovascular surgery: a retrospective study 预测心血管手术术中血液制品输血量：一项回顾性研究

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2026-01-15 DOI: 10.1016/j.transci.2026.104374

Hiroki Shibata , Yuma Hirano , Mikihiro Shimizu , Kaede Ino , Akiho Ikeda , Yui Nagae , Naoki Nemoto , Mizuki Oishi , Takahito Shinba , Chiaki Yamada , Keiko Ishizuka , Takaaki Ono

Background and Objectives

Red blood cells are often overprepared for surgery, leading to waste and increased costs, despite the need for cross-matching tests. This study aimed to develop a model for predicting the number of red blood cell units required during cardiovascular surgery using patient characteristics.

Materials and Methods

This retrospective study included patients who underwent cardiovascular surgery at our hospital from April 2022 to October 2022. Multiple regression analysis was performed using preoperative patient attributes and blood data, with red blood cell units used during surgery as the objective variable. Models were developed using patient demographics and blood data, with additional models incorporating specific surgical procedures to assess predictive accuracy.

Results

Model 1 included age, sex, weight, hematocrit, prothrombin time-international normalized ratio, serum creatinine, volume of preoperatively donated diluted autologous blood, and history of cardiovascular surgery. Model 2 included the same variables as Model 1, plus aortic aneurysm resection as a surgical procedure. The predictive equations of the study showed superior accuracy for both Model 1 and Model 2 compared to the conventional red blood cell units requested by physicians or those predicted using the surgical blood order equation based on correlation coefficients. Model 2 outperformed both Model 1 and conventional methods in predictive utility.

Conclusion

This study developed a clinically useful formula for predicting the number of red blood cell units required during surgery based on preoperative patient-specific data, without restricting the analysis to specific procedures. This formula may improve blood product inventory management and reduce medical costs.

背景与目的尽管需要进行交叉配型试验，但手术时红细胞往往准备过度，导致浪费和成本增加。本研究旨在建立一种模型，利用患者特征预测心血管手术期间所需的红细胞数量。材料与方法回顾性研究纳入2022年4月至2022年10月在我院行心血管手术的患者。采用术前患者属性和血液数据进行多元回归分析，以术中使用的红血球单位为客观变量。使用患者人口统计数据和血液数据建立模型，并结合特定的外科手术过程来评估预测的准确性。结果模型1包括年龄、性别、体重、红细胞压积、凝血酶原时间-国际标准化比值、血清肌酐、术前捐献稀释自体血容量、心血管手术史。模型2包括与模型1相同的变量，加上主动脉瘤切除术作为外科手术。该研究的预测方程显示，与医生要求的传统红细胞单位或基于相关系数的外科血序方程预测的红细胞单位相比，模型1和模型2的准确性更高。模型2在预测效用上优于模型1和传统方法。本研究开发了一个临床有用的公式，可以根据术前患者特异性数据预测手术期间所需的红细胞数量，而不局限于特定的手术过程。该配方可改善血液制品库存管理，降低医疗成本。

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引用次数: 0

Intra-procedure white blood cell monitoring as a predictor of collection efficiency in mononuclear cell apheresis 术中白细胞监测作为单核细胞分离收集效率的预测因子

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2026-01-15 DOI: 10.1016/j.transci.2026.104376

Austin Choi, Shasta Theodore, Yanyun Wu, Yamac Akgun

Background

Efficient collection of cellular starting material is critical for the success of chimeric antigen receptor T-cell (CAR-T) therapy. While hematopoietic progenitor cell (HPC) harvests rely on pre-procedure CD34⁺ counts to predict adequacy, equivalent real-time predictors for mononuclear cell (MNC) collections remain lacking. This study evaluated intra-procedure white blood cell (WBC) concentration as a potential indicator of collection performance.

Methods

A retrospective review was conducted of CARVYKTI MNC apheresis procedures performed between July 2023 and November 2024 at the University of Miami, Sylvester Comprehensive Cancer Center. Institutional Review Board approval was obtained (protocol #20201331). Pre-procedure peripheral WBC counts (ppWBC), intra-procedure WBC concentrations (ipWBC), total blood volume processed (TBVIP), and collection efficiency (CE) were analyzed. Correlations were assessed using Spearman’s rank coefficients.

Results

Forty-seven procedures were analyzed. Median ppWBC was 4.5 × 10³/µL (IQR 3.6–6.4), median ipWBC 75.8 × 10 ³ /µL (IQR 49.4–97.2), and median CE 0.21 (IQR 0.17–0.26). ipWBC showed strong positive correlations with ppWBC (r = 0.68, p = 1.7 ×10⁻⁷) and CE (r = 0.54, p = 9.0 ×10⁻⁵), whereas ppWBC alone did not correlate with CE (r = –0.14, p = 0.35).

Conclusions

Intra-procedure WBC concentration correlates with collection efficiency and provides a more meaningful real-time indicator of MNC harvest performance than pre-procedure counts alone. Incorporating intra-procedure monitoring into apheresis workflows may enhance yield predictability and streamline CAR-T manufacturing logistics

细胞起始材料的有效收集是嵌合抗原受体t细胞（CAR-T）治疗成功的关键。虽然造血祖细胞（HPC）的采集依赖于术前CD34 +计数来预测是否充足，但单核细胞（MNC）收集的等效实时预测指标仍然缺乏。本研究评估了术中白细胞（WBC）浓度作为采集性能的潜在指标。方法回顾性分析2023年7月至2024年11月在迈阿密大学Sylvester综合癌症中心进行的CARVYKTI MNC单采手术。已获得机构审查委员会批准（协议#20201331）。分析术前外周白细胞计数（ppWBC）、术中白细胞浓度（ipWBC）、处理总血容量（TBVIP）和收集效率（CE）。使用Spearman等级系数评估相关性。结果共分析了47例手术。中位ppWBC为4.5 × 10³/µL (IQR 3.6-6.4)，中位ipWBC为75.8 × 10 ³ /µL (IQR 49.4-97.2)，中位CE为0.21 （IQR 0.17-0.26）。与ppWBC ipWBC显示强烈的正相关性(r = 0.68,p = 1.7  ×10⁻⁷)和CE (r = 0.54,p = 9.0 ×10⁻⁵),而单独使用ppWBC没有与CE (r = -0.14,p = 0.35)。结论单工序白细胞浓度与采收效率相关，比单工序白细胞计数更能实时反映采收效果。将程序内监测纳入单采工作流程可以提高产量的可预测性并简化CAR-T制造物流

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引用次数: 0

Feasibility of preparing whole blood-derived pooled buffy coat granulocyte concentrates for pediatric patients 为儿科患者制备全血源性池状褐皮粒细胞浓缩物的可行性

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2026-01-06 DOI: 10.1016/j.transci.2026.104373

Kriti Batni, Satyam Arora, Seema Dua, Anupa Pokhrel, Akshay Paliwal, Arisha Khan

Background

Granulocyte transfusions remain a therapeutic option in managing severe neutropenic sepsis and selected hematological conditions. However, their availability is limited due to challenges in mobilization, collection, and short shelf life. In resource-constrained settings, buffy coat-derived granulocyte pools may serve as a feasible alternative.

Aims

To assess the feasibility of pooling buffy coat (BC) derived granulocytes, validate the pooling process, and compare granulocyte yield between two different pooling strategies (pool of 4 vs. pool of 6 BC)

Materials and Methods

A prospective study was conducted using buffy coats from routine 450 mL whole blood donations (N = 150). BCs were pooled (N = 60 for pool of 4 vs N = 90 for pool of 6 BC-GCs). Donor, individual buffy coat, pooled buffy coat samples were analyzed for volume, complete blood counts, platelet and granulocyte yield. European (EDQM) and UK standards were used as quality benchmarks.

Results

Granulocyte yield, volume, and red cell content were significantly higher in 6-BC pools compared to 4-BC pools (p < 0.001). All 6-BC pools met the EDQM granulocyte dose threshold (>5 × 10⁹/unit), whereas only 33 % of 4-BC pools did. Sterility was maintained throughout. To reduce red cell content for pediatric suitability, 6 RBC-reduced pools underwent high-speed centrifugation. Compared to the unmanipulated pools, RBC-reduced pools demonstrated significantly lower volume and RBC volume and significantly higher granulocyte concentration (42.8 ± 16.1 vs. 24.0 ± 3.1 ×10 ³/µL, p = 0.035) with preserved total granulocyte yield (p = 0.40). However, a key limitation of our study is the lack of functional assays to assess post-processing granulocyte viability and phagocytic activity.

Conclusion

This approach offers a cost-effective, logistically simpler method for generating granulocyte-rich components where apheresis is not feasible. Pooled buffy coat granulocytes can be a viable alternative in settings with limited access to granulocytapheresis, meeting acceptable quality standards for clinical use.

背景：粒细胞输注仍然是治疗严重中性粒细胞减少性败血症和某些血液学疾病的一种治疗选择。然而，由于动员、收集和保质期短的挑战，它们的可用性是有限的。在资源受限的情况下，暗色涂层衍生的粒细胞池可能是一种可行的替代方案。目的评估收集白皮毛（BC）来源的粒细胞的可行性，验证收集过程，并比较两种不同收集策略（4 BC池和6 BC池）的粒细胞产量。材料和方法采用常规450 mL全血（N = 150）的白皮毛进行前瞻性研究。bc被合并（4个bc - gc池N = 60,6个bc - gc池N = 90）。分析供体、个体、集合的褐毛样品的体积、全血细胞计数、血小板和粒细胞产量。欧洲（EDQM）和英国标准被用作质量基准。结果6-BC池的粒细胞产量、体积和红细胞含量显著高于4-BC池（p <； 0.001）。所有6-BC池都符合EDQM粒细胞剂量阈值（>；5 × 10⁹/单位），而4-BC池中只有33 %符合。无菌一直保持着。为了降低儿童红细胞含量，6个红细胞减少池进行高速离心。unmanipulated池相比,RBC-reduced池体积明显降低和红细胞体积和粒细胞浓度显著升高(42.8 ±  16.1和24.0±3.1  ×10 ³/µL p = 0.035)与保存总粒细胞产量(p = 0.40)。然而，我们研究的一个关键限制是缺乏评估处理后粒细胞活力和吞噬活性的功能分析。结论该方法为制备不能进行单采分离的富粒细胞组分提供了一种成本效益高、物流简单的方法。在粒细胞穿刺受限的情况下，集中的灰褐色被粒细胞是一种可行的替代方法，符合临床使用可接受的质量标准。

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引用次数: 0

Recommendation for a new transfusion threshold in pediatric platelet transfusion: Platelet mass measurement 推荐儿科血小板输注的新输血阈值：血小板质量测量

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2026-01-06 DOI: 10.1016/j.transci.2026.104370

Ceren Kılcı , Fatma Burcu Belen Apak , Meriç Ergene , Khaled Warashne , Buket Daldaban Sarıca , Lale Olcay , Figen Özçay , Esra Baskın , İlkay Erdoğan , Ayşenur Ecevit , Pamir Işık

The decision for platelet transfusion in children and newborns is associated with etiology of thrombocytopenia and concomitant comorbid conditions. In recent years, it has been shown that transfusion may be appropriate in newborn cases using platelet mass thresholds obtained by multiplying mean platelet volume (MPV) and platelet count. A number of 183 platelet transfusion administered to 82 patient aged 0–18 years between January 2017 and January 2019 at Başkent University Ankara Hospital were evaluated in our study in terms of transfusion threshold values, transfusion type and efficacy. It was observed that among platelet transfusions, 94 % were prophylactic and 6 % were therapeutic.There was no difference between the appropriateness of transfusion according to platelet mass and the prevention of bleeding and cost (p > 0.05).The number of transfusions that did not meet the platelet mass threshold value was 32 (17.7 %).Considering that conventional platelet transfusion threshold in pediatric cases is lower than in newborn cases (defined as 0-≤30 day old babies), we think that platelet mass threshold values similar to newborns can be used effectively in pediatric cases as well. The transfusion threshold values in our hypothesis may reduce morbidity and cost without reducing its effectiveness.

儿童和新生儿输注血小板的决定与血小板减少症的病因和伴随的合并症有关。近年来，有研究表明，通过血小板平均体积（MPV）和血小板计数相乘得到的血小板质量阈值可能适合新生儿输血。本研究对2017年1月至2019年1月在ba肯特大学安卡拉医院进行的82例0-18岁患者的183次血小板输注进行了输血阈值、输注类型和疗效的评估。观察血小板输注中，94 %为预防性输注，6 %为治疗性输注。根据血小板质量是否适宜输血与预防出血及费用差异无统计学意义（p >； 0.05）。未达到血小板质量阈值的输血32例（17.7 %）。考虑到儿科病例的常规血小板输注阈值低于新生儿病例（定义为0-≤30 日龄婴儿），我们认为类似于新生儿的血小板质量阈值也可以有效地用于儿科病例。我们假设的输血阈值可以在不降低其有效性的情况下降低发病率和成本。

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引用次数: 0

Identification strategy for patients with ABO blood typing difficulties caused by IgM autoantibodies IgM自身抗体致ABO血型困难患者的识别策略

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2026-01-06 DOI: 10.1016/j.transci.2026.104372

Chen Wang , Xian Huang , Lina Wu , Shichun Wang , Pengyu Zhang

Objective

The objective of this study was to investigate the characteristics of autoantibodies and cause of autoantibodies production and clinical blood transfusion strategies in the ABO blood group system by identifying and analysing 2 rare ABO blood group antigen-specific autoantibodies.

Methods

Blood group antigens and antibodies were detected via the conventional tube method, a gel microcolumn assay and the absorptionelution technique. The antibodies were characterized by direct antiglobulin tests and sulfhydryl reagent treatment of the plasma. ABO genotyping was performed using Sanger double-stranded sequencing of the ABO exons. Review the patient's medical records such as medical history and treatment history, and continuously monitor the ABO blood group.

Results

The blood type of the first case was confirmed to be type A₁ by serological and molecular biological tests, with autoanti-A antibodies, the production of this autoantibody was associated with the disease itself; The blood type of the second case was type A₁B, with autoanti-B antibody, the production of this autoantibody was associated with the use of immune checkpoint inhibitors.

Conclusions

ABO blood group autoantibodies should be considered when antibodies against the A or B antigen are suspected in ABO reverse group typing after ruling out the possibility of alloantibodies, subtype antibodies and foreign antibodies. For patients with autoantibodies, appropriate blood products for transfusion should be selected based on the results of cross-matching tests, antibody thermal amplitude, and in-vitro hemolysis tests, and the transfusion process should be closely monitored.

目的通过鉴定和分析2种罕见的ABO血型抗原特异性自身抗体，探讨ABO血型系统中自身抗体的特点、产生原因及临床输血策略。方法采用常规试管法、凝胶微柱法和吸收洗脱法检测血型抗原和抗体。抗体通过直接抗球蛋白试验和血浆巯基试剂处理进行鉴定。采用ABO外显子的Sanger双链测序进行ABO基因分型。复查患者病史、治疗史等病历，持续监测ABO血型。结果1例患者经血清学和分子生物学检查，血型为A1型，具有自身抗a抗体，该自身抗体的产生与疾病本身有关；第二例患者的血型为A1B型，具有自身抗b抗体，该自身抗体的产生与免疫检查点抑制剂的使用有关。结论在排除同种异体抗体、亚型抗体和外源抗体的可能性后，在ABO逆组分型中怀疑存在针对A抗原或B抗原的抗体时，应考虑sabo血型自身抗体。对于自身抗体患者，应根据交叉配型试验、抗体热幅值和体外溶血试验的结果选择合适的输血血制品，并密切监测输血过程。

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引用次数: 0

Mechanisms of immune modulation by therapeutic plasma exchange 治疗性血浆交换的免疫调节机制。

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2026-01-02 DOI: 10.1016/j.transci.2026.104371

Nicholas Parisi, Menatalla Nadim, Yamac Akgun

Therapeutic plasma exchange (TPE) is a cornerstone extracorporeal therapy with broad immunomodulatory effects across diverse immune-mediated and inflammatory diseases. By removing circulating pathogenic substances such as autoantibodies, immune complexes, complement components, and pro-inflammatory cytokines, TPE rapidly interrupts humoral pathways driving tissue injury. This review summarizes the mechanisms through which plasma exchange modulates immune activity, including both depletion of harmful plasma factors and restoration of normal homeostatic proteins when donor plasma is used. Biomarker analyses demonstrate predictable reductions in immunoglobulins, cytokines, and complement activity, reflecting the profound but transient immune reset achieved with TPE. Comparisons with related apheresis modalities, such as immunoadsorption, double filtration plasmapheresis, and extracorporeal photopheresis, highlight the unique advantages of TPE as a broadspectrum immunomodulatory intervention. Clinically, TPE remains a vital therapeutic option in conditions ranging from thrombotic thrombocytopenic purpura and myasthenia gravis to vasculitis and cytokine storm syndromes. As evidence and technology evolve, plasma exchange continues to exemplify how biophysical removal of pathogenic plasma constituents can effectively restore immune balance and improve patient outcomes in both acute and chronic immune-mediated diseases.

治疗性血浆交换（TPE）是一种基础体外治疗方法，在多种免疫介导和炎症性疾病中具有广泛的免疫调节作用。通过清除循环中的致病性物质，如自身抗体、免疫复合物、补体成分和促炎细胞因子，TPE迅速阻断驱动组织损伤的体液通路。本文综述了血浆交换调节免疫活性的机制，包括使用供体血浆时有害血浆因子的消耗和正常稳态蛋白的恢复。生物标志物分析显示免疫球蛋白、细胞因子和补体活性的可预测降低，反映了TPE实现的深刻但短暂的免疫重置。与免疫吸附、双滤浆置换和体外光置换等相关的分离方式进行比较，突出了TPE作为广谱免疫调节干预手段的独特优势。在临床上，TPE仍然是一个重要的治疗选择，从血栓性血小板减少性紫癜和重症肌无力到血管炎和细胞因子风暴综合征。随着证据和技术的发展，血浆交换继续证明生物物理去除致病性血浆成分如何有效地恢复免疫平衡并改善急性和慢性免疫介导疾病患者的预后。

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引用次数: 0

Redistribution ratio in the clearance of environmental toxins by double filtration plasmapheresis 双重过滤血浆置换法清除环境毒素的再分配比

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2026-01-02 DOI: 10.1016/j.transci.2026.104369

Yandy Marx Castillo-Aleman

While double filtration plasmapheresis (DFPP) has been used clinically for years, its role in environmental toxin removal remains underexplored. Even in other apheresis modalities used for poisoning and drug overdose, such as therapeutic plasma exchange and red blood cell exchange, few studies have examined the redistribution of such compounds from peripheral tissues back into plasma. In this context, we introduce the “redistribution ratio” (RR) as a novel metric to quantify this phenomenon. Calculated from circulating and collected pools before and after DFPP, RR offers insight into the post-procedural rebound of target analytes. High RR values suggest redistribution from extravascular compartments, commonly seen with lipophilic toxins or compounds that have a high volume of distribution and low plasma protein binding. In contrast, low or negative RR values may reflect limited redistribution due to intracellular sequestration, metabolic elimination, or irreversible tissue binding. To illustrate its clinical relevance, we present RR patterns from a cohort of patients with hyperlipoproteinemia(a) treated with DFPP, showing heterogeneous patterns across a range of environmental toxins. These findings support RR as a valuable complement to conventional pre- and post-procedure concentration metrics, with potential applications in refining DFPP timing and predicting rebound risk. RR may also serve as a surrogate marker for tissue toxin burden, although further validation is needed across broader toxin classes and apheresis platforms.

虽然双过滤血浆置换（DFPP）已在临床上应用多年，但其在环境毒素去除中的作用仍未得到充分探讨。即使在用于中毒和药物过量的其他分离方式中，如治疗性血浆交换和红细胞交换，也很少有研究检查这些化合物从外周组织重新分布到血浆中。在这种情况下，我们引入了“再分配比率”（RR）作为量化这种现象的新度量。根据DFPP前后的循环和收集池计算，RR提供了对目标分析物的术后反弹的见解。高RR值提示血管外腔室再分布，常见于亲脂毒素或具有高分布体积和低血浆蛋白结合的化合物。相反，低或负的RR值可能反映了由于细胞内隔离、代谢消除或不可逆的组织结合而导致的有限的再分配。为了说明其临床相关性，我们介绍了一组接受DFPP治疗的高脂蛋白血症(a)患者的RR模式，显示了一系列环境毒素的异质性模式。这些研究结果支持RR作为常规术前和术后浓度指标的有价值补充，在优化DFPP时间和预测反弹风险方面具有潜在的应用价值。RR也可以作为组织毒素负荷的替代标记物，尽管需要在更广泛的毒素类别和采血平台上进一步验证。

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引用次数: 0

Association between platelet transfusion units and platelet transfusion refractoriness in patients with hematological disease: A cohort study in China 血液病患者血小板输注单位与血小板输注难治性的关系：中国的一项队列研究

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2025-12-23 DOI: 10.1016/j.transci.2025.104368

Jingwei Zhang , Yujie Kong , Siwei Liu , Liqi Lu , Li Tian

Purpose

Patients with hematologic diseases frequently receive platelet transfusions and exhibit a higher incidence of platelet transfusion refractoriness (PTR). This study assessed whether platelet transfusion units are a risk factor for PTR.

Methods

We enrolled 598 patients from the Hematology Department of Chengdu Second People's Hospital (Feb 2020-Feb 2023). Based on CCI values, patients were categorized into PTR (n = 207) and non-PTR (n = 391) groups. The relationship between platelet transfusion units and PTR risk was analyzed using logistic regression, with adjustments for confounders.

Results

The PTR incidence was 34.61 %. Platelet transfusion units were significantly associated with increased PTR risk (OR=1.93, 95 % CI 1.67–2.24, p < 0.001); after adjusting for age, sex, infection, body mass index, disease classification, red blood cell transfusion units, and routine blood test results, this correlation increased higher (OR=2.35, 95 % CI 1.89–2.91, p < 0.001). The fitting curve between the platelet transfusion units and risk of PTR show a positive linear relationship(p = 0.472) Furthermore, when dividing platelet transfusion units into < 3 Units and ≥ 3 Units, patients receiving ≥ 3 Units had a significantly higher risk of PTR (OR=6.17, 95 % CI 4.24–8.97, p < 0.001) compared to those receiving < 3 Units; similarly, after adjusting for covariates, the risk of PTR in patients receiving ≥ 3 Units was still higher (OR=7.38, 95 % CI 4.29–12.69, p < 0.001). After propensity score matching, the risk remained robust (OR=4.59, 95 % CI 2.77–7.61, p < 0.001) across various model corrections.

Conclusion

Platelet transfusion units independently correlate with PTR incidence, suggesting the need for careful assessment and reduction of unnecessary transfusions.

目的：血液病患者频繁接受血小板输注，血小板输注难治性（PTR）发生率较高。本研究评估血小板输注单位是否是PTR的危险因素。方法：纳入成都市第二人民医院血液科（2020年2月- 2023年2月）598例患者。根据CCI值将患者分为PTR组（n = 207）和非PTR组（n = 391）。采用logistic回归分析血小板输注单位与PTR风险之间的关系，并对混杂因素进行调整。结果：PTR发生率为34.61 %。血小板输注单位与PTR风险增加显著相关（OR=1.93, 95 % CI 1.67-2.24, p ）结论：血小板输注单位与PTR发生率独立相关，提示需要仔细评估并减少不必要的输注。

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引用次数: 0

Bioinformatic applications in blood group molecular typing 生物信息学在血型分子分型中的应用。

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2025-12-23 DOI: 10.1016/j.transci.2025.104300

Andre D. Luchessi , Thomas Sierocinski , Teenus P. Jayaprakash , Celina Montemayor Garcia

The application of molecular laboratory methods to predict the expression of red blood cell antigens is well established in today’s clinical transfusion practice. The advent of high-throughput molecular blood typing approaches – based on microarray, short-read, and long-read next generation sequencing platforms – has set the stage for widespread, high-resolution donor and patient typing, encompassing the entire breadth of known antigens and propelling swift discovery in this field. Realizing these benefits requires not only carefully designed and validated laboratory assays, but also robust computational and software infrastructures for processing, storing, and interpreting the large volume of generated data, which may represent a new challenge for blood collectors and transfusion centers. To support this practice transition and facilitate adoption of these technologies, we review recent literature on genomic blood typing approaches, outline IT infrastructure considerations, describe available software and analytical algorithms, and discuss the emerging role of artificial intelligence in this rapidly evolving space.

应用分子实验室方法预测红细胞抗原的表达在今天的临床输血实践中已经很好地建立了。基于微阵列、短读和长读下一代测序平台的高通量分子分型方法的出现，为广泛、高分辨率的供体和患者分型奠定了基础，涵盖了已知抗原的全部广度，并推动了这一领域的快速发现。实现这些好处不仅需要精心设计和验证的实验室分析，还需要强大的计算和软件基础设施来处理、存储和解释大量生成的数据，这可能对采血人员和输血中心构成新的挑战。为了支持这一实践转变并促进这些技术的采用，我们回顾了基因组血型方法的最新文献，概述了IT基础设施的考虑，描述了可用的软件和分析算法，并讨论了人工智能在这一快速发展的领域中的新兴作用。

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引用次数: 0

The effect of online education on cardiovascular intensive care nurses’ self-efficacy in blood transfusion: A randomized controlled trial conducted in Turkey 在线教育对心血管重症护士输血自我效能的影响：在土耳其进行的一项随机对照试验

IF 1.2 4区医学 Q4 HEMATOLOGY

Transfusion and Apheresis Science

Pub Date : 2025-12-23 DOI: 10.1016/j.transci.2025.104306

Ahmet Songül , Arzu Bahar

Objective

Safe transfusion of blood and blood products is a critical and complex process in cardiovascular surgical intensive care units. This study aimed to determine nurses’ self-efficacy in safe blood transfusion and to examine the effect of a structured online training program.

Method

This randomized controlled study included 90 nurses working in a cardiovascular surgical intensive care unit of a training and research hospital. The intervention group (n = 45) received a 40-minute theoretical and practical online training on safe blood transfusion, while the control group (n = 45) received no training. Data were collected using a Personal Information Form and the Self-Efficacy Scale for Safe Blood and Blood Product Transfusion for Nurses. Analyses were performed with independent-samples t-tests and one-way ANOVA, with a significance level of p < 0.05.

Results

The post-test mean score of the intervention group (215.22 ± 10.17) was significantly higher than that of the control group (197.11 ± 24.47) (p < 0.05). All sub-dimensions—knowledge, procedural skills, and transfusion safety confidence—showed significant improvement in the intervention group. Nurses with higher education levels and younger age demonstrated greater improvements in self-efficacy scores.

Discussion

The structured online training was effective in increasing nurses’ self-efficacy for safe transfusion practices. Demographic factors influenced the learning outcomes.

Conclusion

Structured online training programs improve the knowledge, skills, and confidence of cardiovascular intensive care nurses. It is recommended that such programs be conducted regularly and tailored to demographic differences.

目的在心血管外科重症监护病房中，血液及血液制品的安全输血是一个关键而复杂的过程。本研究旨在确定护士在安全输血中的自我效能感，并检验结构化在线培训计划的效果。方法随机对照研究对象为某培训研究型医院心血管外科重症监护病房的90名护士。干预组（n = 45）接受了40分钟的安全输血理论和实践在线培训，对照组（n = 45）未接受培训。采用《护士安全输血自我效能感量表》和《护士安全输血自我效能感量表》进行数据收集。分析采用独立样本t检验和单因素方差分析，显著性水平为p <； 0.05。结果干预组术后平均得分（215.22 ± 10.17）显著高于对照组（197.11 ± 24.47）（p <； 0.05）。所有子维度——知识、程序技能和输血安全信心——在干预组均有显著改善。受教育程度越高、年龄越小的护士自我效能感得分提高越大。结构化的在线培训在提高护士安全输血实践的自我效能感方面是有效的。人口因素影响学习效果。结论结构化的在线培训方案提高了心血管重症监护护士的知识、技能和信心。建议定期开展此类方案，并根据人口差异进行调整。

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