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Species Composition and Diversity of Coastal Trawled Fishes Landed Off the Coast of Qua Iboe River Estuary, Ibeno, Nigeria 尼日利亚伊贝诺夸伊博河口海岸拖网捕捞鱼类的物种组成和多样性
Pub Date : 2024-07-24 DOI: 10.9734/ajriz/2024/v7i3161
Essien-Ibok, M. A., G. U. U., Ekpo, I. E., Emah, N. M.
An assessment of the specie composition and diversity of fish species from Qua Iboe River Estuary was carried out over a period of six months (March-August, 2019). A total of thirty - one (31) species representing seventeen (17) families were identified under the families Bothidae, Charangidae, Clupeidae, Cynoglossidae, Drepanidae, Eleotridae, Gobiidae, Haemulidae, Polynemidae, Soleidae, Sciaenidae, Sparidae, Sphyraenidae, Scombridae, Tetraodontidae, Trachinidae and Trichuridae. Monthly abundance was lowest in April (122 specimens; 6.21 %) and was highest in March (545 specimens; 27.72 %). The longest species was Trichirus lepturus while the shortest were Drepane africana and Selena dorsalis. Family Sciaenidae had the highest representation in terms of species and Haemulidae had the highest number of individuals. Shannon-Weiner Index of general Diversity (H) value was showing dominance of in Charangidae family. Evenness Index (E) showed all the species were not equally distributed in the waters. Assessment of the condition factor (K) revealed that 22 out of 31 fish species K values less than 5 showing good body condition and wellbeing of the fish while 11 species showed bad body condition. The estuary has a rich ichthyofauna complex. Effective exploitation measures should be used to deter the menace of local extinction in the near future.
对夸伊博河口的鱼类物种组成和多样性进行了为期六个月(2019 年 3 月至 8 月)的评估。共鉴定出三十一(31)种鱼类,代表十七(17)个科,分别属于 Bothidae 科、Charangidae 科、Clupeidae 科、Cynoglossidae 科、Drepanidae 科、Eleotridae 科、Gobiidae 科、Haemulidae 科、Polynemidae 科、Soleidae 科、Sciaenidae 科、Sparidae 科、Sphyraenidae 科、Scombridae 科、Tetraodontidae 科、Trachinidae 科和 Trichuridae 科。月丰度在四月最低(122 个标本;6.21 %),在三月最高(545 个标本;27.72 %)。体长最长的物种是毛滴虫(Trichirus lepturus),体长最短的物种是 Drepane africana 和 Selena dorsalis。就物种而言,Sciaenidae 科的代表性最高,Haemulidae 科的个体数最多。香农-韦纳总体多样性指数(H)显示,夏朗科为优势种。均匀度指数(E)显示所有物种在水域中的分布不均。状态因子(K)评估显示,31 个鱼类物种中有 22 个物种的 K 值小于 5,表明鱼类的身体状况良好和健康,而 11 个物种的身体状况较差。河口有丰富的鱼类种群。应采取有效的开发措施,以遏制不久的将来当地鱼类灭绝的威胁。
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引用次数: 0
Study on the Fish Diversity and Distribution of Chromatophores in Some Fishes Inhabiting Manawar Tawi, Rajouri(J&K), India 印度拉朱里(查谟和克什米尔)马纳瓦尔塔维地区鱼类多样性及部分鱼类色素体分布研究
Pub Date : 2024-07-22 DOI: 10.9734/ajriz/2024/v7i3160
Satinder Kaur, Sarita Raina, S. Jangral
The present study on fish diversity from Manawar Tawi, Rajouri (J&K) was carried out for the period of three months from September 2023 to November 2023. Fishes are very important from biodiversity point of view. Therefore, during the present survey, total six fish species belonging to five genera and two families were collected and identified in this region. Also the distribution of different chromatophores was studied in the fish Labeo boga. This study emphasizes the importance of conducting regular surveys in order to analyse the impact of anthropogenic activities on the fish diversity and impact of pollution on the distribution of chromatophores.
本项关于拉朱里(查谟和克什米尔)马纳瓦尔塔维地区鱼类多样性的研究于 2023 年 9 月至 2023 年 11 月进行,为期三个月。从生物多样性的角度来看,鱼类非常重要。因此,在本次调查中,共收集并鉴定了该地区的五属两科六种鱼类。此外,还研究了 Labeo boga 鱼体内不同色素的分布情况。这项研究强调了进行定期调查的重要性,以便分析人类活动对鱼类多样性的影响以及污染对嗜铬体分布的影响。
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引用次数: 0
Effect of Locally Available Feed on the Zootechnical Performance of Local Chickens in Niger 当地饲料对尼日尔当地鸡的动物技术性能的影响
Pub Date : 2024-06-15 DOI: 10.9734/ajriz/2024/v7i3155
Brah Nouri, Moussa Hassan Ousseini, Issa Salissou
This study aimed to investigate the impact of incorporating locally available ingredients into the diet of young indigenous chickens in Niger on their growth performance. Two hundred chickens, aged 6 weeks, were utilized in the study and randomly assigned to four groups of ten birds each, housed on peanut shell bedding. The diets were formulated using a variety of local ingredients including millet, wheat bran, millet bran, peanut cake, sorrel seeds, fish meal, locust meal, moringa leaves, and bone meal. Water was provided ad libitum to all birds, and the feeding trial lasted for 4 months. Results revealed that the third diet was the most preferred by the local chickens. The diet significantly influenced the live weight of the chickens in the second month (P=0.000) and showed a significant effect during the third month (P=0.020) of the experiment. Sexual dimorphism was observed between hens and roosters for diets 2, 3, and 4. Statistical analysis indicated a significant difference in the Average Daily Gain of local chickens among the different treatments during the first month. Additionally, the diet significantly influenced the consumption index of local chickens during the first month of the experiment (P=0.055). Based on the findings, it is suggested that Diet 2, containing fish meal, can be utilized in areas where fish availability and accessibility are high. Conversely, Diets 3 and 4 may be more suitable for regions prone to locust infestations. To facilitate the adoption of these dietary formulations, it is recommended to replicate the experiments in rural settings.
本研究旨在调查在尼日尔本土幼鸡的日粮中添加当地食材对其生长性能的影响。研究利用了 200 只 6 周龄的鸡,将其随机分配到四组,每组 10 只,饲养在花生壳垫料上。日粮由多种当地原料配制而成,包括小米、麦麸、小米麸、花生饼、苏木籽、鱼粉、蝗虫粉、辣木叶和骨粉。所有鸟类均可自由饮水,饲喂试验持续了 4 个月。结果显示,当地鸡最喜欢第三种日粮。在试验的第二个月,日粮对鸡的活重有明显影响(P=0.000),在第三个月也有明显影响(P=0.020)。在日粮 2、3 和 4 中观察到母鸡和公鸡的性别二形性。统计分析表明,在第一个月中,不同处理的本地鸡平均日增重存在显著差异。此外,在试验的第一个月,日粮对本地鸡的消耗指数也有显著影响(P=0.055)。根据研究结果,建议在鱼类供应量和可获得性较高的地区使用含有鱼粉的日粮 2。相反,日粮 3 和 4 可能更适合蝗虫多发地区。为促进这些日粮配方的采用,建议在农村环境中重复实验。
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引用次数: 0
A Study of Age, Sex Ratio, Gonado-Somatic Index, Fecundity, and Breeding Season, of Nile Tilapia (Oreochromis niloticus Linnaeus, 1758) from Khashm El-Girba Reservoir and Atbara River, Eastern Sudan 苏丹东部 Khashm El-Girba 水库和 Atbara 河尼罗罗非鱼(Oreochromis niloticus Linnaeus, 1758)的年龄、性别比、性腺指数、繁殖力和繁殖季节研究
Pub Date : 2024-06-12 DOI: 10.9734/ajriz/2024/v7i3154
Mutasim Yousif Mohamed Abdalla, Abu Elgasim Ibrahim Abd Elhalim, Mujtaba EL-Khair Shuaib, Abdalla Mustafa Hamid, Noha Ahmed Sati, Moatez Mohammed Elhassan, A. E. Adam
This study aims to investigate some reproductive aspects of Nile tilapia, Oreochromis niloticus in the Khashm El-Girba reservoir and Atbara River, eastern Sudan. The study was conducted during December 2015 to November 2016. Samples were collected bi-monthly basis from three localities, namely; Um Aswad (Atbara River), El-Remila, and El-Monaba (Khashm El-Girba reservoir), using beach seine nets of 1.2 cm 4.0 cm and 7.0 cm stretched mesh. A total of 1595 O. niloticus specimens were collected from all sampling sites. The age of O. niloticus was estimated using five anterior vertebrae of fish body; The results indicate that the age ranged between 0+ to 4+ years. Most of the collected fish specimens were of age 1+, with females most dominant at this age. Sex ratio was 1:1(M: F). Gonado-somatic index (GSI) of females of O. niloticus ranged between 1.965 – 2.620, and from 0.434 – 0.439 for males. Two periods of increase of GSI were observed reflecting two breeding seasons of this species. Used gravimetric method to estimate the average fecundity of O. niloticus ranged between 481 eggs in Um Aswad sampling site, and 638 in El-Remila site. The fecundity of O. niloticus has a high correlation with gonadal weight (r=0.82708) but a low correlation with standard length (r=0.47612) and total weight (r=0.50874).The results of this investigation on O. niloticus indicate that the age of the fish ranged from 0+ to 4+ years, with a sex ratio of 1:1. The Gonado-somatic Index (GSI) indicated two spawning seasons, and fecundity was found to be strongly correlated with gonad weight, and moderately correlated with total length and standard length. This results can be used formulate management policies and procedures for protecting and conserving the fisheries resources and to regulate and improve the catches of the most important commercial fish species in that region.
本研究旨在调查苏丹东部 Khashm El-Girba 水库和 Atbara 河中尼罗罗非鱼(Oreochromis niloticus)的一些繁殖问题。研究于 2015 年 12 月至 2016 年 11 月期间进行。使用 1.2 厘米、4.0 厘米和 7.0 厘米拉伸网眼的滩涂围网,每两个月从三个地点采集样本,即 Um Aswad(阿特巴拉河)、El-Remila 和 El-Monaba(Khashm El-Girba 水库)。在所有采样点共采集到 1595 尾黑线鳕标本。使用鱼体的五个前椎骨估算了黑线鳕的年龄;结果表明,黑线鳕的年龄在 0+ 至 4+ 岁之间。大部分采集到的鱼类标本年龄在 1 岁以上,雌鱼在这个年龄段占多数。性别比例为 1:1(男:女)。雌鱼的性腺指数(GSI)在 1.965 - 2.620 之间,雄鱼在 0.434 - 0.439 之间。观察到两个时期的 GSI 上升,反映了该物种的两个繁殖季节。使用重力法估算的黑线鳕平均受精率在 Um Aswad 采样点为 481 枚卵,El-Remila 采样点为 638 枚卵之间。黑线鳕的繁殖力与性腺重量(r=0.82708)有很高的相关性,但与标准体长(r=0.47612)和总重量(r=0.50874)的相关性较低。性腺指数(GSI)显示有两个产卵季节,繁殖力与性腺重量密切相关,与总长度和标准长度适度相关。这一结果可用于制定管理政策和程序,以保护和养护渔业资源,并调节和提高该地区最重要的商业鱼类的产量。
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引用次数: 0
A Review on Bioinformatics and Genomic Advances in Black Widow Spider Research 黑寡妇蜘蛛研究中的生物信息学和基因组学进展综述
Pub Date : 2024-06-06 DOI: 10.9734/ajriz/2024/v7i3153
Aakriti Shrivastava, V. K. Sharma
Sustainable ecosystem is huge topic for discussion in this era. It's a continuous process that comes from some biotic and abiotic factors such as; pollution of the atmosphere, waste production, toxic substance disposal, damaged aquatic ecosystems, deforestation, etc. To control the loss of biodiversity we must apply some innovative technologies of genome analysis and database management systems. Spiders (order Araneae) are the species which is broadly distributed in our diversity. Latrodectus is a genus of amazing spiders commonly known as a black widow have numerous attractive features and is a huge research topic, Because of its biological product and behavior. The literature review has been carried out by following reasoning methodology and is presented. The results of this study indicate that different researchers have taken different approaches to analysing the genomic data with some broadly use methods like whole genome sequencing, genotyping, and different proteome analysis and management of data Overall, it is evident from the literature offers a meaningful option, especially for managing genomic data and provided new framework towards the sustainable ecosystem.
可持续生态系统是当今时代讨论的一个重要话题。这是一个持续的过程,来自一些生物和非生物因素,如大气污染、废物生产、有毒物质处理、水生生态系统破坏、森林砍伐等。为了控制生物多样性的丧失,我们必须应用基因组分析和数据库管理系统等创新技术。蜘蛛(蛛形纲)是在我们的多样性中分布广泛的物种。Latrodectus是一种神奇的蜘蛛属,俗称黑寡妇,具有众多吸引人的特征,由于其生物产品和行为,是一个巨大的研究课题。本文采用推理方法进行了文献综述。这项研究的结果表明,不同的研究人员采取了不同的方法来分析基因组数据,其中一些广泛使用的方法包括全基因组测序、基因分型以及不同的蛋白质组分析和数据管理。总之,从文献中可以明显看出,这些方法提供了一种有意义的选择,特别是在管理基因组数据方面,并为实现可持续生态系统提供了新的框架。
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引用次数: 0
Predilection Site for the Meat Lover Trichinella spp Larvae and its Pathogenesis and Potency in Human Host 肉食动物毛旋毛虫幼虫的偏好部位及其在人类宿主体内的致病机理和效力
Pub Date : 2024-05-15 DOI: 10.9734/ajriz/2024/v7i2150
F. E. Siagian
Aims: To revisited carefully previously published articles available in the internet regarding predilection site of Trichinella spp larvae including their pathogenesis and its potency. Discussion: By carefully reviewing previously published literature available in the internet regarding the parasite Trichinella tropism to its host’s striated muscle, the author briefly withdraw some important keypoint to be revealed. The nematode Trichinella spp causes serious zoonosis called trichinellosis, a disease affecting striated or skeletal muscles which consider as one of tropical disease. Even though its natural host varied, but infection among live stocks, such as pigs and other animals, which are raising public health concern. Human infection occurs after consumption of raw or undercooked meat or meat products contain muscle larvae of T. spiralis. The tropism of the parasite for individual striated muscles and/or striated muscle groups varies significantly. Trichinella spp. has a direct life cycle where all three life cycle stages (the infective muscle larvae, adult, and new born larvae) happen serially in one host only. Intestine-dwelling adults of Trichinella produce newborn larvae that bypass the enterocyte, enter the bloodstream and colonize skeletal muscle. The muscle larvae assemble excretory-secretory products which play crucial role in establishing and maintaining persistent parasitism and the host’s immune modulation and evasion. It turns out that excretory-secretory products from muscle larvae and mature worm also have hidden medical potential that can be used to treat allergic problems, inflammation-based diseases, autoimmunity and even malignancy. Conclusion: Trichinellosis is a serious and potentially fatal zoonosis which transmitted through consuming raw or uncooked contaminated meat or its comestibles. Its primary tropism is to the host’s striated muscle and infection can persist for a long time facilitated by several reciprocities of its product (e.g., excretory-secretory) with the host’s cell and immune system. Fortunately, there are several promising potency in the field of therapeutic and prevention medicine which should be explored intensely.
目的:仔细研究以前在互联网上发表的有关旋毛虫幼虫偏好部位的文章,包括其致病机理及其效力。讨论:通过仔细查阅以前在互联网上发表的有关寄生虫旋毛虫对宿主横纹肌的趋性的文献,作者简要地提出了一些需要揭示的重要观点。旋毛虫属线虫会引起严重的人畜共患疾病--旋毛虫病,这是一种影响横纹肌或骨骼肌的疾病,被视为热带疾病之一。尽管其自然宿主多种多样,但在猪和其他动物等活畜中的感染引起了公共卫生的关注。人类在食用了含有螺旋体肌肉幼虫的生肉或未煮熟的肉类或肉制品后会受到感染。寄生虫对个别横纹肌和/或横纹肌群的趋性差异很大。旋毛虫有一个直接的生命周期,所有三个生命周期阶段(感染性肌肉幼虫、成虫和新生幼虫)都只在一个宿主体内连续发生。寄居在肠道中的旋毛虫成虫会产生新生幼虫,这些幼虫会绕过肠道细胞,进入血液并在骨骼肌中定植。肌肉幼虫组装排泄分泌物,这些分泌物在建立和维持持续寄生以及宿主的免疫调节和规避方面发挥关键作用。事实证明,肌肉幼虫和成熟蠕虫的排泄物-分泌物也具有隐藏的医疗潜力,可用于治疗过敏问题、炎症性疾病、自身免疫甚至恶性肿瘤。结论毛霉菌病是一种严重的、可能致命的人畜共患病,通过食用生的或未煮熟的受污染肉类或其零食传播。它的主要滋生部位是宿主的横纹肌,其产物(如排泄物-分泌物)与宿主的细胞和免疫系统之间的几种互惠作用可使感染持续很长时间。幸运的是,在治疗和预防医学领域,有几种很有前景的药物值得深入研究。
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引用次数: 0
The Effect of Purwoceng Extract on the Etawa Crossbreed Bucks Mating 茜草提取物对伊塔瓦杂交雄鹿交配的影响
Pub Date : 2024-05-06 DOI: 10.9734/ajriz/2024/v7i2149
Ikhsan Abdilla Yahya, S. Sitepu, Adhona Bhajana Wijaya Negara
Aims: This research aims to determine the effect of purwoceng extract on Etawa crossbreed bucks Study Design:  Completely randomized non-factorial design with 4 treatments given to bucks (ethawa crossbreeds), namely Purwoceng (Pimpinella alpina KDS or Pimpinella pruatjan Molk.)  extract Place and Duration of Study: Ikhsan Farm, Sei Glugur Village, Pancur Batu District, Indonesia, between December 2023 and March 2024. Methodology: The research was carried out by administering various doses of purwoceng extract to Etawa crossbreed bucks and then observing mating with Etawa crossbreed doe. The treatment given was administration of purwoceng extract in amounts P0 (0 mg), P1 (400 mg), P2 (800 mg), and P3 (1400 mg). The research parameters were the frequency of mounting does, the frequency of penetration, the number of doe mounted and the number of doe penetrated. Results: There was an increase in the values of all parameters. The lowest frequency of mounting doe is P0, namely 17 and the highest is P3, namely 32. The lowest frequency of penetration is P0, namely 12 and the highest is 20. The lowest number of doe mounted is P0 and P1, namely 5 and the highest is P3, namely 7. The lowest number of doe penetrations is P0 and P1 are 5 and the highest are P2 and P3, namely 6. Conclusion: The best treatment is the addition of 800 mg of purwoceng extract (P2), Because the highest number of penetrating doe has the same value between P2 and P3, it will be more efficient if the treatment used is P2.
研究目的:本研究旨在确定麝香草提取物对伊塔瓦杂交雄鹿的影响 研究设计: 完全随机非因子设计,对雄鹿(伊达瓦杂交种)进行 4 种处理,即紫云英(Pimpinella alpina KDS 或 Pimpinella pruatjan Molk.)提取物:2023 年 12 月至 2024 年 3 月:印度尼西亚 Pancur Batu 区 Sei Glugur 村 Ikhsan 农场。研究方法:研究方法是给伊塔瓦杂交雄鹿注射不同剂量的马钱子提取物,然后观察雄鹿与伊塔瓦杂交雌鹿的交配情况。给雄鹿的处理剂量分别为 P0(0 毫克)、P1(400 毫克)、P2(800 毫克)和 P3(1400 毫克)。研究参数为母鹿上马频率、插入频率、上马母鹿数量和插入母鹿数量。结果所有参数值都有所增加。骑乘母鹿频率最低的是 P0,即 17 次,最高的是 P3,即 32 次。穿刺频率最低的是 P0,即 12 次,最高的是 20 次。安装母鹿数量最少的是 P0 和 P1,即 5 只,最多的是 P3,即 7 只;穿刺母鹿数量最少的是 P0 和 P1,即 5 只,最多的是 P2 和 P3,即 6 只。 结论:添加 800 毫克马钱子提取物(P2)是最好的治疗方法,因为 P2 和 P3 的母鹿穿透次数最高值相同,所以使用 P2 治疗会更有效。
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引用次数: 0
Investigation into the Impacts of Varied Batches of Stripped Eggs on the Reproductive Success of Clarias gariepinus 调查不同批次的剥离卵对赤鲤繁殖成功率的影响
Pub Date : 2024-03-23 DOI: 10.9734/ajriz/2024/v7i2146
Otoh, A. J., George, U. U., Asangusung, P. S., Okoko, A. C., Ekanem, I. E., Umaha, M. N.
This study was conducted at the hatchery complex of Akwa Ibom State University to investigate the effects of different batches of stripped egg on reproductive performances of Clarias gariepinus. Nine brood stocks of Clarias gariepinus (six males and 3 females) with average body weight of 2.3kg and 64.2 cm where carefully selected for the study. Six matured males were euthanized for the removal of testis without hormonal inducements. The milt collected from the six males were pooled together and divided into 12 portions each diluted with 2mL of normal saline solution. Three female brood stocks where separately induced with hormone at single dosage of 0.5ml/kg body weight and allowed for a period of 10 hours before stripping. Stripping of eggs from each brood stock where in four batches, each batch measured 50g and labeled A, B, C and D. 3g of eggs containing approximately 2000 oocytes (eggs) where measured out from each batch and mixed with the diluted milt and incubated in a 2 x 1 x 10cm3 of water in a concrete pond at temperature of 26oC in three replicates. The result revealed that the reproductive performances considered in this study decreased as the batches of stripped eggs increased from 1 ̶ 4 batches. The first batch of stripped eggs produced 87.69 ± 0.42, 97.06 ± 0.84, 84.29 ± 1.05 percentage fertilization, hatchability and survival rate respectively, while the least batch of stripped eggs (batch 4) produced the least value of fertilization 50.33 ± 1.67, 31.93 ± 4.94 hatchability and survival rate value of 42.69 ± 0.61.  Therefore, from the above result, the least batch of stripped eggs should not be used for fertilization because the unhatched or dead eggs has a negative effect on the fertilized eggs including fry at the hatchery level.
这项研究在阿夸伊博姆州立大学的孵化场进行,目的是调查不同批次的剥离卵对金眼鲷繁殖性能的影响。本研究精心挑选了平均体重为 2.3 千克、体长为 64.2 厘米的九尾鲤鱼(六雄三雌)。六条成熟的雄鱼被安乐死,在没有激素诱导的情况下切除睾丸。将从这六只雄鱼身上收集到的睾丸液集中起来,分成 12 份,每份用 2 毫升生理盐水稀释。分别对三只雌性育雏种群进行激素诱导,单次剂量为 0.5 毫升/千克体重,诱导 10 小时后再剥离。从每批育雏种群中剥离的卵分四批,每批 50 克,分别标记为 A、B、C 和 D。从每批中取出 3 克含约 2000 个卵母细胞(卵)的卵,与稀释的乳汁混合,在温度为 26 摄氏度的混凝土池塘中放入 2 x 1 x 10 立方厘米的水中孵化,分三个重复。结果显示,随着剥离卵的批次从 1 ̶ 增加到 4 批,本研究中考虑的繁殖性能有所下降。第一批剥离卵的受精率、孵化率和成活率分别为 87.69 ± 0.42、97.06 ± 0.84 和 84.29 ± 1.05,而最少批次剥离卵(第 4 批)的受精率为 50.33 ± 1.67、孵化率为 31.93 ± 4.94,成活率为 42.69 ± 0.61。 因此,从上述结果来看,不应使用最少批次的剥离卵进行受精,因为未孵化卵或死卵对孵化水平的受精卵(包括鱼苗)有负面影响。
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引用次数: 0
Comparative Study of Amino Acids in the Different Stages of Honey Bee 蜜蜂不同阶段氨基酸的比较研究
Pub Date : 2024-03-21 DOI: 10.9734/ajriz/2024/v7i2145
M. S. M. Lakshmi, T. E. T. Sunitha
Apiculture is the practice of keeping bees as well as the manufacturing of honey and beeswax. Nectar is the main source of carbohydrates for honey bees. The amount of nectar needed pet colony depends on how concentrated the sugars are in the nectar. A worker bee needs 11 mg of dry sugar each day, which translates to about 22 µl of 50% sugar syrup per bee per day. In order to make honeybees grow satisfactorily enough feed must be provided, and feed should have all the necessary nutrients for the growth of honeybees. The important nutrients such as amino acids, lipids, carbohydrates, and requirements of vitamins and minerals are necessary for the growth and development of honeybees. During development, several structural, physiological, and biochemical changes occur takes place in an organism from stage to stage. There is a variation in the quality of all biochemical components. In the present study, the free amino acids from the eggs, larvae, pupae, and adult honeybee Apis cerana indica and also honey have been analyzed. This work was carried out by adopting the technique of paper chromatography. The developed chromatograms were dried and the spots were identified by spraying with (O.IN) Ninhydrin in acetone. The Rf values were calculated and compared with the Rf values of the standard amino acids. The presence of amino acids in different life stages of honeybee and honey were analyzed and compared.
养蜂业是饲养蜜蜂以及制造蜂蜜和蜂蜡的行业。花蜜是蜜蜂碳水化合物的主要来源。蜂群所需的花蜜量取决于花蜜中糖的浓度。工蜂每天需要 11 毫克干糖,相当于每只蜜蜂每天需要约 22 微升 50%的糖浆。为了使蜜蜂生长得令人满意,必须提供足够的饲料,饲料中应含有蜜蜂生长所需的所有营养物质。蜜蜂生长发育所需的氨基酸、脂类、碳水化合物、维生素和矿物质等重要营养成分是必不可少的。在蜜蜂的生长发育过程中,不同阶段的有机体在结构、生理和生化方面都会发生一些变化。所有生化成分的质量都存在差异。本研究分析了蜜蜂卵、幼虫、蛹和成蜂以及蜂蜜中的游离氨基酸。这项工作采用纸色谱法进行。色谱图经干燥后,用丙酮中的(O.IN)茚三酮喷洒,对斑点进行鉴定。计算 Rf 值,并与标准氨基酸的 Rf 值进行比较。分析并比较了不同生命阶段的蜜蜂和蜂蜜中氨基酸的含量。
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引用次数: 0
Effects of Multiple Batches of Stripped Eggs on the Reproductive Performances of Heterobranchus longifilis 多批剥离卵对长尾异鳃鱼繁殖性能的影响
Pub Date : 2024-03-14 DOI: 10.9734/ajriz/2024/v7i2144
Otoh, A. J., Okoko, A. C., Ekanem, I. E., George, U. U., Asangusung, P. S.
Studying the effects of multiple batches of stripped eggs contributes to the sustainability and viability of aquaculture practices by improving our understanding of reproductive performance and informing best practices in breeding programs, hence, the need to investigate on the effects of multiple batches of stripped egg on reproductive performances of Heterobranchus logifilis became necessary. The aim was to provide valuable information for decision-making processes in aquaculture operations. Nine brood stocks of H. longifilis (6 male & 3 female) with average body weight of 2.3 kg and average length of 64.2 cm where carefully selected for the study. Six matured males were sacrificed for the removal of testis without hormonal inducements. The milt collected from the six males were pulled together and divided into twelve portions each diluted with 2ml of normal saline solution. Three female brood stocks where separately induced at single dosage of 0.5ml/kg body weight and allowed for a period of 10 hours before stripping.  Stripping of eggs from each brood stock where in four batches, each batch measured 50g and labeled A, B, C & D. 3g of eggs containing approximately 2000 oocytes (eggs) where measured out from each batch and mixed with the diluted milt and incubated in a 2 x 1 x 10cm3 of water in a concrete pond at temperature of 26oC in three replicates. The result revealed that the reproductive performances considered in this study decreased as the batches of stripped eggs increased from 1- 4 batches. The first batch of stripped eggs produced 90.17 ± 0.44%, 97.78±0.86%, 89.48 ±1.08% fertilization, hatchability and survival respectively, while the least batch of stripped eggs (batch 4) produced the least value of fertilization (5.83 ± 1.69%); 45.00±4.90% hatchability and survival value of 5.59 ± 0.61%.  Sequel to the findings of the present study, the least batch of stripped eggs should be avoided for use in fertilization since the unhatched or dead eggs has some detrimental effects on the fertilized eggs including fry at the hatchery level.
研究多批剥离卵的影响有助于提高我们对繁殖性能的了解,并为育种计划的最佳实践提供信息,从而促进水产养殖实践的可持续性和可行性,因此有必要研究多批剥离卵对 Logifilis 异鳃鳕繁殖性能的影响。其目的是为水产养殖操作的决策过程提供有价值的信息。本研究精挑细选了九尾长吻鲈的育雏种群(6 雄 3 雌),平均体重 2.3 千克,平均体长 64.2 厘米。在不使用激素诱导的情况下,牺牲了六只成熟的雄鱼以切除睾丸。将从这六只雄鱼身上收集到的睾丸分成十二份,每份用 2 毫升生理盐水稀释。分别用 0.5 毫升/千克体重的剂量诱导三只雌性育雏种群,诱导 10 小时后再剥离。 从每个育雏种群中剥离的卵分四批,每批 50 克,分别标记为 A、B、C 和 D。从每批中取出 3 克卵,约含 2000 个卵母细胞(卵),与稀释后的乳汁混合,在混凝土池塘中 2 x 1 x 10 立方厘米的水中孵化,温度为 26 摄氏度,分三个重复。结果显示,随着剥离卵的批次从 1-4 批增加,本研究中考虑的繁殖性能有所下降。第一批剥离卵的受精率、孵化率和存活率分别为 90.17±0.44%、97.78±0.86%、89.48±1.08%,而最少批次剥离卵(第 4 批)的受精率最低(5.83±1.69%),孵化率为 45.00±4.90%,存活率为 5.59±0.61%。 根据本研究的结果,应避免使用最少批次的剥离卵进行受精,因为未孵化卵或死卵会对受精卵(包括孵化水平的鱼苗)产生不利影响。
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Asian Journal of Research in Zoology
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