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Effectiveness of Ethanol Gel and Essential Oil of Kencur Rhizome (Kaempferia galanga L.) base Karbopol against Propionibacterium acnes 卡波波尔碱基 Kencur Rhizome(Kaempferia galanga L.)乙醇凝胶和精油对痤疮丙酸杆菌的功效
Pub Date : 2024-01-01 DOI: 10.25134/quagga.v16i1.81
Jurnal Pendidikan, dan Biologi, Rahayu Samalo, Tri Gunaedi, D. Y. Runtuboi
Kencur rhizome (Kaempferia galanga L.) is an aromatic herbal plant as antibacterial and anti-inflammatory. This study aims to determine the physical quality and effectiveness of gel preparations of ethanol extract and essential oil of kencur rhizome based on carbopol inhibiting Propionibacterium acnes bacteria. This study used a Randomized Block Design (RAL) with 2 different treatments of 5 levels each, namely ethanol extract gel treatment and essential oil extract gel with concentrations of 10%, 20%, 30%, 40% and 50%. The results of the gel formulation of extracts and essential oils based on carbopol showed pH, texture, spreadability and adhesiveness according to the quality standards of topical preparations. The antibacterial inhibition of the gel was moderate at 50% extract concentration (11.41 mm); and 50% essential oil (9.04 mm). Positive control treatment inhibited growth by 15.84 mm while negative control showed no response. The extract gel and essential oil of galangal rhizome had a significant effect but were less effective in inhibiting P. acnes bacteria. Adding the maximum concentration of extract and essential oil can optimize acne healing.
肯库尔根茎(Kaempferia galanga L.)是一种芳香草本植物,具有抗菌消炎的作用。本研究旨在确定基于carbopol的Kencur根茎乙醇提取物和精油凝胶制剂的物理质量和对痤疮丙酸杆菌的抑制效果。本研究采用随机区组设计(RAL),有2种不同的处理,每种处理有5个水平,即乙醇提取物凝胶处理和精油提取物凝胶处理,浓度分别为10%、20%、30%、40%和50%。结果显示,基于 carbopol 的提取物和精油凝胶配方的 pH 值、质地、涂抹性和粘附性均符合外用制剂的质量标准。在 50%提取物浓度(11.41 毫米)和 50%精油浓度(9.04 毫米)下,凝胶的抗菌抑制作用适中。阳性对照处理的抑菌效果为 15.84 毫米,而阴性对照则没有任何反应。高良姜根茎提取物凝胶和精油对抑制痤疮丙酸杆菌有显著效果,但效果较差。添加最大浓度的提取物和精油可以优化痤疮的治疗效果。
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引用次数: 0
Selection of Proteolytic Bacteria from Bonoloyo Public Burial Place (PBP), Banjarsari, Surakarta, Central Java 从中爪哇苏腊卡尔塔省 Banjarsari 的 Bonoloyo 公共墓地 (PBP) 挑选蛋白水解细菌
Pub Date : 2024-01-01 DOI: 10.25134/quagga.v16i1.57
Putri Salwa Salsabilla, Y. Sidiq, Erma Musbita Tyastuti, Triastuti Rahayu
Public Burial Place (PBP) is a cemetery for everyone. In PBP, protein decomposition occurs actively because the human body is made up of 16.4% protein, so it is most likely that proteolytic bacteria can be found. Previous research has isolated as many as 45 isolates from PBP Bonoloyo, Surakarta, but has not selected their proteolytic capabilities. The aim of the study was to isolate proteolytic bacteria from the Bonoloyo Public Cemetery and identify them. The bacterial isolate of PBP Bonoloyo is a collection from the FKIP Biological Laboratory of Muhammadiyah University of Surakarta that selected its proteolytic capabilities using the milk agar scheme (SMA). The parameters measured are the diameter of the colony and the lymphatic zone formed to obtain the value of the proteolytic index (IP). For bacterial isolates showing a positive proteolytic activity, simple identification is carried out through the observation of colonial morphology and Gramme colouring. The results of the study showed that as many as 28 isolates (62.2%) were positive for proteolytic activity, with IP values >2 for B1 and B3. Bacterial proteolytic isolates are dominantly white to yellow with irregular, filamentary, spindle, and circular colonial forms. The results of Gramme colouring show that bacterial isolates that have proteolytic abilities are a group of Gramme negative bacteria that are basil shaped. The bacterial isolate that shows the highest IP value is the B1 isolate, which is suspected to be of the genus Bacillus. This research obtained data showing that in the public cemetery (PBP), Bonoloyo stores potential as a proteolytic bacterial habitat for a group of Gramme negative bacteria.
公共墓地(PBP)是每个人的墓地。在 PBP 中,蛋白质分解发生活跃,因为人体由 16.4% 的蛋白质组成,所以最有可能发现蛋白质分解细菌。以前的研究从苏腊卡尔塔的波诺洛约 PBP 分离出了多达 45 种分离菌,但没有对它们的蛋白分解能力进行筛选。本研究的目的是从 Bonoloyo 公共墓地分离出蛋白水解细菌并对其进行鉴定。Bonoloyo 公共墓地的细菌分离物是从苏腊卡尔塔穆罕默迪亚大学 FKIP 生物实验室收集的,该实验室使用牛奶琼脂方案(SMA)对其蛋白水解能力进行了筛选。测量的参数是菌落直径和形成的淋巴区,以获得蛋白水解指数(IP)值。对于蛋白水解活性呈阳性的细菌分离物,可通过观察菌落形态和革兰氏染色进行简单鉴定。研究结果表明,多达 28 个分离物(62.2%)的蛋白水解活性呈阳性,其中 B1 和 B3 的 IP 值大于 2。蛋白水解细菌主要呈白色至黄色,有不规则、丝状、纺锤形和环状菌落。革兰氏染色的结果表明,具有蛋白水解能力的细菌分离物是一组革兰氏阴性细菌,呈玄武岩状。IP 值最高的细菌分离物是 B1 分离物,疑似属于芽孢杆菌属。这项研究获得的数据显示,在公共墓地(PBP)中,Bonoloyo 有潜力成为一组革兰氏阴性细菌的蛋白水解细菌栖息地。
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引用次数: 0
Inventarization Macroscopic Fungi at Tegallega Resort Taman Gunung Gede Pangrango National Park Tegallega Resort Taman Gunung Gede Pangrango 国家公园的大型真菌编目工作
Pub Date : 2024-01-01 DOI: 10.25134/quagga.v16i1.51
Nabila Khusnul Cahyaningtyas, Husnin Nahry
This study aims to inventory macroscopic fungi at Resort Tegallega, Gunung Gede Pangrango National Park conducted at three tracking locations. The research method used is descriptive qualitative and with the cruise data collection method (cruise method) by tracing the research location points.The results obtained based on the research that has been done, it was found that most of the fungi belong to the phylum Basidiomycota and only one type of fungus belongs to the phylum Ascomycota. The environmental conditions where mushrooms grow are not that much different, because the three tracking points are still in the same area.
本研究旨在盘点古农吉德潘兰戈国家公园泰加莱加度假村的大型真菌,在三个追踪地点进行。研究结果表明,大部分真菌属于担子菌门(Basidiomycota),只有一种真菌属于子囊菌门(Ascomycota)。蘑菇生长的环境条件差别不大,因为三个追踪点仍在同一地区。
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引用次数: 0
The Effect of Thiamine HCL Concentration Variation on the Seedling of Phalaenopsis Var. Happy Valentine 硫胺素 HCL 浓度变化对蝴蝶兰变种幼苗的影响快乐情人节
Pub Date : 2024-01-01 DOI: 10.25134/quagga.v16i1.91
Var. Happy, Valentine Handini, M. A. Dzakiy, Eny Hartadiyati
Phalaenopsis var. Happy Valentine orchid is a plant with a growth and flowering process of approximately 1-2 years. Generally, orchids can bloom in about 9-12 months. Optimal concentrations of Thiamine HCl (vitamin B1) are required to support the vegetative growth of Phalaenopsis var. Happy orchids. This research aims to determine the effect and the best concentration of vitamin B1 among 1ml, 2ml, and 3ml on the vegetative growth, including leaf span, root quantity, leaf width, and length of young leaves of Phalaenopsis var. Happy Valentine seedlings. The method used was a Completely Randomized Design (CRD), with treatment factors being the concentration of vitamin B1 and Growmore fertilizer, comprising four treatment levels and six replications, namely V0 = 2gr/L Growmore fertilizer, V1 = 2gr/L Growmore fertilizer + 1ml B1, V2 = 2gr/L Growmore fertilizer + 2ml B1, dan V3 = 2gr/L Growmore fertilizer + 3ml B1. This research used 24 Phalaenopsis var. Happy Valentine orchid seedlings as research samples. The data were analyzed using ANOVA with a significance level 0.05 and subsequently subjected to Duncan's test. The results indicate that vitamin B1 significantly affects leaf span, length of young leaves, and the quantity of roots in Phalaenopsis var. Happy Valentine.
蝴蝶兰变种幸福情人节兰花是一种生长和开花过程约为 1-2 年的植物。一般来说,兰花可在 9-12 个月左右开花。要支持蝴蝶兰变种幸福情人兰的无性生长,需要最佳浓度的硫胺素盐酸盐(维生素 B1)。本研究旨在确定 1 毫升、2 毫升和 3 毫升维生素 B1 对蝴蝶兰变种幸福情人兰幼苗的无性生长(包括叶展、根量、叶宽和幼叶长度)的影响和最佳浓度。采用的方法是完全随机设计(CRD),处理因子为维生素 B1 和生长茂肥料的浓度,包括四个处理水平和六个重复,即 V0 = 2gr/L 生长茂肥料、V1 = 2gr/L 生长茂肥料 + 1ml B1、V2 = 2gr/L 生长茂肥料 + 2ml B1、Dan V3 = 2gr/L 生长茂肥料 + 3ml B1。本研究使用了 24 株蝴蝶兰变种幸福情人节兰花幼苗作为研究样本。数据采用方差分析,显著性水平为 0.05,然后进行邓肯检验。结果表明,维生素 B1 对蝴蝶兰的叶展、幼叶长度和根的数量有明显影响。
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引用次数: 0
Effect of Problem-Based Learning Integrated with Ethnoscience on Critical Thinking Skills in SMA Negeri 01 Kubu 与民族科学相结合的问题式学习对 SMA Negeri 01 Kubu 批判性思维能力的影响
Pub Date : 2024-01-01 DOI: 10.25134/quagga.v16i1.54
Mitha Gita Isvida, Ari Sunandar, Hanum Mukti Rahayu
This research was motivated by the low critical thinking skills of students at SMAN 01 Kubu. The aim of this research is to determine the effect of the model problem-based learning integrated Ethnic on students' critical thinking skills. Sampling uses statistical tests and Effect size. The research sample was students of classes X Mia 1 and X Mia 2 at SMAN 01 Kubu. Data collection uses the Essay test method which refers to Fascione's theory. Based on the research results obtained in the experimental class, the score for the synthesis skills indicator was 71.42%, problem recognition and solving skills 74.28%, evaluation or judging skills 51.42%, analysis skills 80%, and concluding skills 60%. In the control class, scores were for synthesis skills 54.28%, problem recognition and solving skills 14.28%, evaluation or judgment skills 22.85%, analysis skills 60%, and concluding skills 40%. In calculating the effect size, a value of 1.54 was obtained, which is in the very high category.
本研究的动机是,SMAN 01 Kubu 学生的批判性思维能力较低。本研究旨在确定基于问题的综合民族学习模式对学生批判性思维能力的影响。抽样采用统计检验和效应大小。研究样本是 SMAN 01 Kubu 的 X Mia 1 班和 X Mia 2 班的学生。数据收集采用了参照 Fascione 理论的论文测试法。根据实验班的研究结果,综合能力指标得分率为 71.42%,认识和解决问题能力得分率为 74.28%,评价或判断能力得分率为 51.42%,分析能力得分率为 80%,总结能力得分率为 60%。在对照班中,综合技能得分率为 54.28%,认识和解决问题的技能得分率为 14.28%,评价或判断技能得分率为 22.85%,分析技能得分率为 60%,总结技能得分率为 40%。在计算效应大小时,得出的数值为 1.54,属于非常高的类别。
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引用次数: 0
Growth of Endophytic Bacteria from Klutuk Banana Plant (Musa balbisiana Colla) with Inoculation Trial on Rice Plants (Oryza sativa) 克鲁图克香蕉植物(Musa balbisiana Colla)内生细菌的生长与水稻植物(Oryza sativa)接种试验
Pub Date : 2024-01-01 DOI: 10.25134/quagga.v16i1.53
Anggun Dwi, Nur Annisa, Triastuti Rahayu, Y. Sidiq
Four potential bacteria have been isolated from Klutuk banana plant. They exhibited IAA production. Since the bacterial inoculation to plant should be performed in exponential phase of bacterial growth, information of the growth is crucial. This study aimed to analyze the growth rate of eight isolates and examine the effect of bacterial inoculation by initial trial on rice plants. For this purpose, four endophytic bacteria from Klutuk banana were cultured and examined using 600nm spectrophotometer. The growth of the isolates was calculated every two hours for 16 hours bacterial growth in the nutrient broth medium. The inoculation trial of bacteria to rice plants was conducted with three replicates. This inoculation was begun by germinating the rice seed and the radicle was observed. Then, the germinated rice seedlings were soaked in the bacterial suspension for 5 minutes in room temperature. The results showed that the exponential phase of all isolates was observed 6 hours after shaking. Inoculation using 6-hour-incubated K7 isolate significantly improved the length of root of rice plants. Additionally, this isolate improved the number of root and shoot length without significant difference. Thus, these results provide important information of the growth phase and inoculation effect new isolated endophytic and rhizosphere bacteria.
从 Klutuk 香蕉植物中分离出了四种潜在的细菌。它们都能产生 IAA。由于对植物的细菌接种应在细菌生长的指数期进行,因此生长信息至关重要。本研究旨在分析八种分离菌的生长速度,并通过在水稻植株上的初步试验来检验细菌接种的效果。为此,从 Klutuk 香蕉中培养了四种内生细菌,并使用 600nm 分光光度计进行检测。在营养肉汤培养基中细菌生长 16 小时,每两小时计算一次分离菌的生长情况。对水稻植株进行了三次细菌接种试验。首先对水稻种子进行发芽,观察胚根。然后,在室温下将发芽的水稻秧苗浸泡在细菌悬浮液中 5 分钟。结果表明,所有分离菌株在振荡 6 小时后都进入了指数期。接种经 6 小时培养的 K7 分离物后,水稻植株根的长度明显增加。此外,该分离物对根的数量和芽的长度也有改善,但无明显差异。因此,这些结果为了解新分离的内生细菌和根瘤菌的生长阶段和接种效果提供了重要信息。
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引用次数: 0
Development of a Mini-research-based Digital Module Using Sigil in Endocrinology Learning 在内分泌学学习中使用 Sigil 开发基于微型研究的数字模块
Pub Date : 2024-01-01 DOI: 10.25134/quagga.v16i1.42
Rahma Widiantie, Ina Setiawati, Anna Fitri Hindriana, Edi Junaedi, Elsa Chintya
Developing technology-based teaching materials that are more innovative, creative, effective, efficient and contextual is a demand in learning. Lecturers are required to be able to develop media and teaching materials by utilizing technological advances. The aim of this research is to develop complete teaching materials or modules consisting of materials, student worksheets, evaluations, assessment techniques and learning videos to improve students' mastery of concepts using the sigil application. The characteristics of the module developed is a digital module based on mini research, where mini research learning can improve students’, problem solving abilities and integrated science process skills. The method in this research is the Design and Development Research method with the PPE model which includes 3 stages: Planning, Production, and Evaluation. The results of the research are digital modules based on mini PPE research models, planning by analyzing the need to design flowcharts and story boards from digital modules, production by creating digital modules, namely compiling the module framework and endocrinology material in Microsoft Word software (html format) the file is opened in the software sigil editor is saved in epub format, evaluation by material experts and media experts by assessing the feasibility of the digital sigil module based on mini research in endocrinology learning. The results of the validity test based on the appropriate aspects of content, language and presentation are valid. The results of the practicality test by students on the aspects of content, language and usefulness are valid. Mini-research-based digital modules using sigil software are valid, practical and suitable for use in Endocrinology courses with the advantage of facilitating thinking skills and increasing student learning independence.
开发更具创新性、创造性、实效性、高效性和情境性的科技教材是学习的需求。要求讲师能够利用先进技术开发媒体和教材。本研究的目的是开发完整的教学材料或模块,包括材料、学生工作表、评价、评估技术和学习视频,以提高学生对使用 sigil 应用程序的概念的掌握程度。所开发模块的特点是基于微型研究的数字化模块,微型研究学习可以提高学生解决问题的能力和综合科学过程技能。本研究采用的方法是设计与开发研究法,PPE 模型包括三个阶段:该模型包括三个阶段:计划、制作和评价。研究成果是基于微型 PPE 研究模型的数字模块,规划是通过分析数字模块设计流程图和故事板的需求;制作是创建数字模块,即用 Microsoft Word 软件(html 格式)编译模块框架和内分泌学材料,该文件在软件 sigil 编辑器中打开后保存为 epub 格式;评估是由材料专家和媒体专家评估基于微型研究的数字 sigil 模块在内分泌学学习中的可行性。基于内容、语言和表现形式等适当方面的有效性测试结果是有效的。学生在内容、语言和实用性方面的实用性测试结果是有效的。使用 sigil 软件的基于微型研究的数字模块是有效的、实用的,适合在内分泌学课程中使用,具有促进思维能力和提高学生学习自主性的优势。
{"title":"Development of a Mini-research-based Digital Module Using Sigil in Endocrinology Learning","authors":"Rahma Widiantie, Ina Setiawati, Anna Fitri Hindriana, Edi Junaedi, Elsa Chintya","doi":"10.25134/quagga.v16i1.42","DOIUrl":"https://doi.org/10.25134/quagga.v16i1.42","url":null,"abstract":"Developing technology-based teaching materials that are more innovative, creative, effective, efficient and contextual is a demand in learning. Lecturers are required to be able to develop media and teaching materials by utilizing technological advances. The aim of this research is to develop complete teaching materials or modules consisting of materials, student worksheets, evaluations, assessment techniques and learning videos to improve students' mastery of concepts using the sigil application. The characteristics of the module developed is a digital module based on mini research, where mini research learning can improve students’, problem solving abilities and integrated science process skills. The method in this research is the Design and Development Research method with the PPE model which includes 3 stages: Planning, Production, and Evaluation. The results of the research are digital modules based on mini PPE research models, planning by analyzing the need to design flowcharts and story boards from digital modules, production by creating digital modules, namely compiling the module framework and endocrinology material in Microsoft Word software (html format) the file is opened in the software sigil editor is saved in epub format, evaluation by material experts and media experts by assessing the feasibility of the digital sigil module based on mini research in endocrinology learning. The results of the validity test based on the appropriate aspects of content, language and presentation are valid. The results of the practicality test by students on the aspects of content, language and usefulness are valid. Mini-research-based digital modules using sigil software are valid, practical and suitable for use in Endocrinology courses with the advantage of facilitating thinking skills and increasing student learning independence.","PeriodicalId":497495,"journal":{"name":"Quagga: Jurnal Pendidikan dan Biologi","volume":"33 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139455762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Quagga: Jurnal Pendidikan dan Biologi
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