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Viability of Methicillin-Resistant Staphylococcus aureus (MRSA) Bacteria Preserved at Different Temperature and Time 在不同温度和时间下保存的耐甲氧西林金黄色葡萄球菌(MRSA)的活力
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.60
Jurnal Pendidikan, dan Biologi, Sekar Karima Rachma, Kurniawan, Ikhsan Mujahid, Kurnia Ritma Dhanti
The bacteria Staphylococcus aureus, a normal component of human flora, is easily mutated and antibiotic-resistant, leading to the creation of Methicillin-Resistant Staphylococcus aureus (MRSA). This bacterium is a difficult-to-control nosocomial infection in healthcare settings, so it must be stored using specialized preservation agents, like DMSO, to investigate the nature and characteristics of these bacteria for prevention, control, and therapy. The purpose of this study was to evaluate the survival of MRSA bacteria that had been kept in a DMSO solution at various temperatures and periods. This study was carried out between July and October2022 using a one-shot study case research design, data analysis, and quantitative descriptive analysis. According to the findings, MRSA bacteria held in 15% DMSO solution lost viability over time and at various storage temperatures. Among various bacterial species held at various storage temperatures, MRSA bacteria had the lowest colony loss rate (-20 °C). Based on the findings of this study, it can be said that the preservation of MRSA bacteria at freezing temperature (-20 °C) and storage length of one month resulted in the greatest survivability of the bacteria when stored in 15% DMSO solution.
金黄色葡萄球菌是人体菌群的正常组成部分,但很容易发生变异并对抗生素产生耐药性,从而导致耐甲氧西林金黄色葡萄球菌(MRSA)的产生。这种细菌是医疗机构中一种难以控制的院内感染,因此必须使用专门的保存剂(如二甲基亚砜)进行保存,以研究这些细菌的性质和特点,从而达到预防、控制和治疗的目的。本研究的目的是评估在不同温度和时间段内保存在 DMSO 溶液中的 MRSA 细菌的存活率。本研究于 2022 年 7 月至 10 月间进行,采用了一次研究个案的研究设计、数据分析和定量描述性分析。研究结果表明,在 15%二甲基亚砜溶液中保存的 MRSA 细菌会随着时间的推移和不同的保存温度而失去活力。在不同储存温度下保存的各种细菌中,MRSA 细菌的菌落损失率最低(-20 °C)。根据这项研究的结果,可以说在冷冻温度(-20 °C)下保存 MRSA 细菌,并在 15%的 DMSO 溶液中保存一个月后,细菌的存活率最高。
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引用次数: 0
Analysis and Reconstruction of Student Practicum Worksheets on Food Carbohydrate Testing 食品碳水化合物检测学生实习作业单的分析与重构
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.341
Ghurrotul Bariroh, Widi Purwianingsih, Kusnadi
Practicum worksheets are a tool used by students to increase their understanding of various biological concepts through experiments and direct observation. However, there are many biology practicum worksheets used today often do not meet the appropriate standards. Therefore, this research aims to analyze conceptual, procedural, and knowledge construction aspects based on vee diagrams on students' practicum worksheets. The method used in this research is qualitative-descriptive. The samples were selected using purposive sampling so that six samples of practicum worksheets for phase F students in the Merdeka Curriculum were obtained with material testing carbohydrates in food. The research results show that conceptually, the practicum content is not in accordance with the learning outcomes in the Merdeka Curriculum. Procedurally, the practicum worksheets still need to be improved in terms of tools, materials, procedures, phenomenon objects, and data recording. This has an impact on students' difficulties in constructing their knowledge. It is hoped that the results of the reconstruction of practicum worksheets based on conceptual, procedural, and knowledge construction analysis can be used as a reference and basis for teachers in designing practicum activities specifically on digestive system material.
实习作业单是学生通过实验和直接观察加深对各种生物概念理解的一种工具。然而,目前使用的许多生物实习作业纸往往不符合相应的标准。因此,本研究旨在根据学生实习作业纸上的维氏图,从概念、程序和知识建构等方面进行分析。本研究采用定性描述法。样本的选取采用了目的取样法,因此获得了六份默迪卡课程 F 阶段学生的实习作业纸样本,其中有测试食物中碳水化合物的材料。研究结果表明,从概念上看,实践内容与默迪卡课程的学习成果不符。从程序上看,实践作业单在工具、材料、程序、现象对象和数据记录方面仍需改进。这对学生构建知识的困难造成了影响。希望基于概念、程序和知识建构分析的实践作业单重建结果,能为教师设计专门针对消化系统材料的实践活动提供参考和依据。
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引用次数: 0
Development of E-LKPD based on Problem Based Instruction on Human Digestive System Material for Class XI Students of SMA Negeri 5 Metro 为 SMA Negeri 5 Metro 的十一年级学生开发基于问题的人体消化系统教材的 E-LKPD
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.133
Jurnal Pendidikan, dan Biologi, Dinda Wardhani, Tika Mayang
The examination was directed to figure out Issue Based Guidance Understudy Worksheets (LKPD) in science examples and to figure out understudies' reactions. The kind of exploration being done by specialists is Improvement (Innovative work). This examination utilizes the Borg and Nerve advancement model embraced by Sugiyono. The instruments utilized are validator and understudy surveys. Then, the information got will be broke down from every appraisal by validator specialists and understudies. The nature of understudy worksheets is completed by approving understudy worksheet items in light of issue based guidance, in particular media specialists, material specialists and language specialists. In light of media specialists, a typical rate was gotten of 84%, material specialists acquired a normal level of 90%, and language specialists got a typical level of 75%. Then, the understudy worksheet item is tried. To figure out understudies' reactions to understudy worksheet items, limited scope preliminaries and huge scope preliminaries were done. Limited scope preliminaries got a typical level of 87% with the standards "Entirely Possible" while in enormous scope preliminaries got a typical level of 87 % with the rules "Truly Qualified" accordingly the understudy worksheet depends on issue based guidance in science examples. created appropriate for use.
这项研究的目的是在科学范例中找出基于问题的指导学生工作表(LKPD),并了解学生的反应。专家们正在进行的探索是改进(创新工作)。这项研究采用了杉野(Sugiyono)所接受的博格和神经促进模型。使用的工具是验证器和学生调查。然后,验证专家和受试者将从每次评估中获得的信息进行分解。学生工作表的性质是根据基于问题的指导,特别是媒体专家、材料专家和语言专家的指导,通过批准学生工作表项目来完成的。就媒体专家而言,典型比率为 84%,材料专家的正常水平为 90%,语言专家的典型水平为 75%。然后,试用学生工作表项目。为了了解学生对学生工作表项目的反应,进行了有限范围的预试和大范围的预试。在有限范围的预赛中,以 "完全可能 "为标准的典型水平为 87%,而在巨大范围的预赛中,以 "真正合格 "为标准的典型水平为 87%。
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引用次数: 0
Development of Interactive Flipbook-Based Learning Media on Cell Material Class XI Students SMA N 01 Kotagajah 开发基于细胞材料的交互式翻页书学习媒体 十一年级学生 SMA N 01 Kotagajah
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.134
Jurnal Pendidikan, dan Biologi, Sinta Ayu Dewi, Hifni Septina Carolina, Institut Agama Islam, Negeri Metro, Jl. Ki, Hajar Dewantara, Iringmulyo No.15A, Kec. Metro Timur, Metro
21st century education requires teachers to be able to utilize technological advances and be more innovative in providing media to support learning activities. In fact, the use of media in the learning process has not been varied and interactive for students. The purpose of the research conducted is to produce an Interactive Flipbook-based learning media on cell material for class XI SMA N 01 Kotagajah students that is feasible to use. This research uses the research and development method, namely Research and Development (R&D) the development model used is the ADDIE model which has five stages, namely: analysis, design, development, implementation, and evaluation. The results of the study showed that the results of validation on material experts obtained a percentage of 88% and media experts reached a percentage of 92% with very feasible criteria, this was supported by the results of responses by teachers who obtained a percentage of 90% in the "very good" category and students got a percentage of 84% in the good category. It can be stated that the Interactive Flipbook-based learning media on cell material for class XI SMA N 01 Kotagajah is very feasible to use.
21 世纪的教育要求教师能够利用先进的技术,在提供媒体支持学习活动方面更具创新性。事实上,学生在学习过程中使用媒体的方式并不多样,互动性也不强。本研究的目的是为十一年级 SMA N 01 Kotagajah 班的学生制作可行的基于细胞材料的互动式翻页书学习媒体。本研究采用研究与开发方法,即研究与开发(R&D),所使用的开发模型是 ADDIE 模型,该模型分为五个阶段,即分析、设计、开发、实施和评估。研究结果表明,教材专家的验证结果为 88%,媒体专家的验证结果为 92%,标准非常可行;教师的回答结果也支持这一标准,"非常好 "的比例为 90%,"好 "的比例为 84%。可以说,基于细胞材料的交互式翻页本学习媒体在十一年级 SMA N 01 Kotagajah 班的使用是非常可行的。
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引用次数: 0
Inventory of Macroscopic Fungi in Protected Forest Areas Malaka District Chartery 马拉卡地区夏洛特森林保护区大型真菌调查
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.315
Jurnal Pendidikan, dan Biologi, Lukas Pardosi, Natalia Hoar, Gergonius Fallo, I. Gede, Arya Wiguna
Mushrooms are eukaryotic organisms with spores, without chlorophyll that reproduce sexually and asexually, mushrooms can be used by humans as food ingredients and ingredients for traditional and modern medicine. The purpose of this study was to determine the type and habitat characteristic of macroscopic fungi conducted in the kateri procted forest area Malaka Regency. Using exploration and  identification methods where the plant identification process is a process of matching a plant according to a particular taxonomi  on each research transect line. There are 28 species of macroscopic fungi namely Dacrymyces stilatus, Auricularia auricula-judae, Xylaria hypoxylon, Daldinia concentrica, Microporus xanthopus, Earliella scabrosa, Pycnoporus sanguineus, Polyporus alveolaris, Lentinus squarrosulus, Hexagonia tenuis, Trametes pubescens, Tyromyces chioneus, Marasmius oreades, Marasmius haematocephalus, Baespora myosura, Marasmius rotula, Marasmius andorsaceus, Trogia infundibiliformis, Leucocoprinus brebissonii, Lepiota helveola. Agaricus micromegathus, Bolbitius vitelinus, Mycena albicocapilaris, Crepidotus variabilis, Parasola plicatilis, Coprinopsis fragilisimus, Lycoperdon pyriforme, Pleurotus djamor. Divided into 5 Orders namely Dacrymycetales, Auriculariales, Xylariales, Polyporales, Agaricales. And 12 family namely Dacrymycetaceae, Auriculariaceae, Xylariaceae, Polyporaceae, Marasmiaceae, Agaricaceae, Bolbitiaceae, Mycenaceae, Crepidotaceae, Psathyrellaceae, Schizophyllaceae, Pleurotaceae. The most abundant habitat Characteristics were on rotten wood branches as many 9 species, on dead  wood stem many as 8 species, in leaf little many as 4 species and in soil with hums many as 7 species.
蘑菇是一种真核生物,有孢子,无叶绿素,可以有性和无性繁殖。本研究的目的是确定马拉卡地区卡特里保护林区大型真菌的类型和栖息地特征。采用探索和识别方法,其中植物识别过程是在每条研究横断线上根据特定分类标准匹配植物的过程。共有 28 种大型真菌,分别是 Dacrymyces stilatus、Auricularia auricula-judae、Xylaria hypoxylon、Daldinia concentrica、Microporus xanthopus、Earliella scabrosa、Pycnoporus sanguineus、Polyporus alveolaris、Lentinus squarrosulus、Hexagonia tenuis, Trametes pubescens, Tyromyces chioneus, Marasmius oreades, Marasmius haematocephalus, Baespora myosura, Marasmius rotula, Marasmius andorsaceus, Trogia infundibiliformis, Leucocoprinus brebissonii, Lepiota helveola.Agaricus micromegathus、Bolbitius vitelinus、Mycena albicocapilaris、Crepidotus variabilis、Parasola plicatilis、Coprinopsis fragilisimus、Lycoperdon pyriforme、Pleurotus djamor。分为 5 目,即 Dacrymycetales 目、Auriculariales 目、Xylariales 目、Polyporales 目、Agaricales 目。12 个科,分别是刺五加科(Dacrymycetaceae)、金针菜科(Auriculariaceae)、木耳菜科(Xylariaceae)、多孔菌科(Polyporaceae)、马齿苋科(Marasmiaceae)、姬松茸科(Agaricaceae)、大戟科(Bolbitiaceae)、真菌科(Mycenaceae)、糙皮菌科(Crepidotaceae)、茜草科(Psathyrellaceae)、五味子科(Schizophyllaceae)、 Pleurotaceae。最丰富的栖息地特征是朽木枝上有 9 种,枯木茎上有 8 种,叶片上有 4 种,有腐殖质的土壤中有 7 种。
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引用次数: 0
Student STEM Literacy Ability Profile on Environmental Change Issues 学生在环境变化问题上的 STEM 素养能力概况
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.344
Syifa Nurifa Dewi, Kusnadi, Y. Hamdiyati
In addressing environmental issues, it is important to measure STEM literacy achievement to increase students' environmental awareness and equip them with the necessary skills to contribute to finding solutions. This research aims to determine the profile of students' STEM literacy achievement in Bandung regarding environmental change issues. The research method used is descriptive with a qualitative approach. The research subjects comprised 193 tenth-grade students selected from SMA Negeri 1 Bandung, SMA Negeri 4 Bandung, and SMA Negeri 9 Bandung for the 2023/2024 academic year, chosen through simple random sampling. The research instrument consisted of 40 multiple-choice questions on environmental change issues, covering seven STEM literacy indicators: explaining phenomena scientifically, evaluating and designing scientific investigations, interpreting data and evidence scientifically, understanding technological principles, developing solutions to achieve goals, formulating situations mathematically, and using concepts, facts, procedures, or reasoning. Data analysis was conducted by calculating the average score of students' STEM literacy achievement per indicator as a percentage. The research results indicate that the percentage of students' STEM literacy achievement is 70%, categorized as moderate. The percentages per STEM literacy indicator are as follows: explaining phenomena scientifically at 71%, evaluating and designing scientific investigations at 61%, interpreting data and evidence scientifically at 73%, understanding technological principles at 76%, developing solutions to achieve goals at 74%, formulating situations mathematically at 61%, and using concepts, facts, procedures, or reasoning at 61%.
在解决环境问题的过程中,重要的是要衡量 STEM 扫盲成果,以提高学生的环境意识,使他们掌握必要的技能,为找到解决方案做出贡献。本研究旨在确定万隆市学生在环境变化问题方面的 STEM 素养成就概况。采用的研究方法是定性描述法。研究对象包括通过简单随机抽样选取的 193 名十年级学生,他们分别来自万隆第一中学(SMA Negeri 1 Bandung)、万隆第四中学(SMA Negeri 4 Bandung)和万隆第九中学(SMA Negeri 9 Bandung),就读于 2023/2024 学年。研究工具包括40道有关环境变化问题的选择题,涵盖七项STEM素养指标:科学地解释现象、评估和设计科学调查、科学地解释数据和证据、理解技术原理、制定解决方案以实现目标、用数学方法制定情境,以及使用概念、事实、程序或推理。数据分析是以百分比的形式计算学生在 STEM 素养方面每个指标的平均成绩。研究结果表明,学生的 STEM 素养成绩百分比为 70%,属于中等水平。每个科学、技术、工程和数学素养指标的百分比如下:科学地解释现象为 71%,评估和设计科学调查为 61%,科学地解释数据和证据为 73%,理解技术原理为 76%,制定解决方案以实现目标为 74%,用数学方法制定情境为 61%,使用概念、事实、程序或推理为 61%。
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引用次数: 0
Comparative Study of Learning Models and Teaching Materials on Student Cognitive Learning Outcomes 学习模式和教材对学生认知学习成果的比较研究
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.127
Jurnal Pendidikan, dan Biologi, Nisa Sholehah Pangsuma, Wahyu Surakusumah, Yayan Sanjaya
Increasing student learning outcomes is one of the benchmarks in evaluating the implementation of the learning process. This research aims to test the effectiveness of learning models and approaches (PBL, PjBL, STEM) as well as teaching materials (E-modules and textbooks) in improving student learning outcomes. Where learning models and approaches and several types of teaching materials will be compared on student learning outcomes. So we will know the interaction of learning models and approaches and teaching materials used in influencing student learning outcomes. The research method used is comparative with experimental design. The samples used were 6 research classes selected using purposive sampling techniques. Research findings show that there is a significant influence between learning models and teaching materials on student learning outcomes, especially on the topic of biodiversity. Therefore, it is important to choose learning models and teaching materials that are relevant to the learning objectives.
提高学生的学习成绩是评估学习过程实施情况的基准之一。本研究旨在测试学习模式和方法(PBL、PjBL、STEM)以及教材(电子模块和教科书)在提高学生学习成果方面的有效性。我们将比较学习模式和方法以及几种类型的教材对学生学习成果的影响。因此,我们将了解学习模式和方法与所用教材在影响学生学习成果方面的相互作用。采用的研究方法是实验设计比较法。使用目的性抽样技术选取了 6 个研究班级作为样本。研究结果表明,学习模式和教材对学生的学习效果有显著影响,尤其是在生物多样性主题方面。因此,选择与学习目标相关的学习模式和教材非常重要。
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引用次数: 0
Analysis of Soil Properties Variations on Various Slope Directions on the National Vegetation Ecosystem of Mount Ciremai, West Java 西爪哇希雷迈山国家植被生态系统不同坡向的土壤特性变化分析
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.128
Agus Yadi, Cecep Kusmana, E. Sudiana, Sukron Aminudin, Mai Fernando Nainggolan
Mount Ciremai National Park has an area of ±15,500 ha with soil characteristics at various heights and varied slope directions. This study aims to determine the characteristics of soil properties in Gunung Ciremai National Park based on slope direction and its relationship with vegetation growth. The method used in this study is a systematic sampling method based on height intervals on four slopes with depths of 0-15 cm and 16-30 cm. The results showed 1). Soil pH in all four slopes with different heights in the acid-neutral category, 2). The soil texture is classified as sandy-loam and sandy-clay loam, 3). C-organic content is classified as low-very high, 4). The content of N-Total is classified as low-high. 5). P2O5 content is classified as very low-low, 6). K content is classified as low-very high, 7). Ca content is low-very high, and 8). Mg content is classified as low to high. Significant correlation tests of soil physical and chemical properties are found on several parameters such as C/N ratio, K, and Mg.
希雷迈山国家公园面积为±15,500公顷,其土壤特性在不同高度和不同坡向上各不相同。本研究旨在根据坡向确定古农吉雷迈山国家公园的土壤特性及其与植被生长的关系。本研究采用的方法是系统取样法,基于四个坡度的高度间隔,深度分别为 0-15 厘米和 16-30 厘米。结果表明 1).四个不同高度斜坡的土壤 pH 值均为中性偏酸性;2).土壤质地分为砂质壤土和砂质粘土壤土,3).有机碳含量为低-极高,4)。氮-总含量为低-高。5).5). P2O5 含量为极低-低,6).钾含量为低-极高,7).Ca 含量为低-极高,8).镁含量从低到高。土壤物理和化学性质的相关性测试结果表明,C/N 比、钾和镁等参数与土壤物理和化学性质有显著相关性。
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引用次数: 0
Test the Effectiveness of Soursop Leaf Extract and Tapak Dara Leaf Extract as Mosquito Larvicide 测试番荔枝叶提取物和塔帕克达拉叶提取物作为蚊子幼虫杀虫剂的效果
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.332
Jurnal Pendidikan, dan Biologi, Syifa Zulfiani, Zakiyah Susilo, Aminah Asngad
In this rainy season the risk of contracting Dengue Fever increases. Many people control dengue fever chemically. The use of chemical larvicides has many disadvantages such as environmental pollution, resistance, and negative impacts on human health. This research aims to develop a natural larvicide from extracts of soursop leaves (Annona muricata) and tapak dara leaves (Catharanthus roseus). The study used a completely randomized design, with three replications. Soursop leaf and tapak dara leaf extracts were extracted using a maceration process using 70% ethanol solvent. III-IV instar Aedes aegypti mosquito larvae were used during the experiment, to assess the effect of formula (3 levels), dose (3 levels), and exposure time for 12 hours. All data were analyzed using Two-way ANOVA. The research results showed that the F1 formula and D3 dose were effective in eradicating mosquito larvae. Where the higher the concentration of soursop leaf extract and the higher the dose, the higher the mortality of mosquito larvae.
在这个雨季,感染登革热的风险会增加。许多人用化学方法控制登革热。使用化学杀幼虫剂有许多缺点,如环境污染、抗药性和对人类健康的负面影响。本研究旨在从酸浆树叶(Annona muricata)和Tapak dara叶(Catharanthus roseus)的提取物中开发一种天然杀幼虫剂。研究采用完全随机设计,三次重复。使用 70% 的乙醇溶剂,通过浸渍法提取酸枣仁叶和塔帕克达拉叶提取物。实验中使用埃及伊蚊三至四龄幼虫,评估配方(3 个水平)、剂量(3 个水平)和暴露时间(12 小时)的影响。所有数据均采用双向方差分析。研究结果表明,F1 配方和 D3 剂量能有效消灭蚊子幼虫。其中,酸果树叶提取物的浓度越高,剂量越大,蚊子幼虫的死亡率就越高。
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引用次数: 0
The Effect of Discovery Learning Model Assisted by Liveworksheet on Biology Learning Independence of Class XI SMA Liveworksheet 辅助发现式学习模式对十一年级 SMA 生物学习独立性的影响
Pub Date : 2024-07-01 DOI: 10.25134/quagga.v16i2.316
Ika Sri, Arsad Bahri, Muhiddin Palennari
This research is a quasi-experimental research that aims to determine the effect of discovery learning model assisted by liveworksheets on the independence of biology learning of high school class XI students. The population in this study were all students of class XI MIPA SMAN 14 Makassar. The research sample was selected randomly (random sampling) so that two classes were obtained, namely class XI MIPA 6 as an experimental class that used the discovery learning model assisted by liveworksheets and class XI MIPA 1 as a control class with 33 students each. Data collection was carried out by giving a questionnaire of learning independence as many as 30 statement items with four answer choices. Data were analyzed by descriptive statistics and inferential statistics. The results of descriptive statistical analysis showed that the average value of the posttest in the experimental class was 86.09 higher than the average value of the control posttest which was 73.48. This study used covariate analysis (Anacova). Based on the results of inferential analysis obtained sig 0.001 smaller than 0.05. It can be concluded that the discovery learning model assisted by liveworksheets has an effect on the learning independence of biology students in class XI SMA.
本研究是一项准实验研究,旨在确定活页辅助发现式学习模式对高中十一年级学生生物学习独立性的影响。研究对象是望加锡 MIPA SMAN 14 高中十一年级的所有学生。研究样本是随机抽样的,因此有两个班级,即使用活页辅助发现学习模式的实验班 XI MIPA 6 班和对照班 XI MIPA 1 班,每个班级有 33 名学生。数据收集是通过发放一份学习自主性问卷进行的,该问卷有 30 个陈述项,四个答案选项。数据通过描述性统计和推论性统计进行分析。描述性统计分析结果显示,实验班的后测平均值为 86.09,高于对照班的后测平均值 73.48。本研究采用了协变量分析法(Anacova)。根据推理分析的结果,sig 0.001 小于 0.05。由此可以得出结论,liveworksheets辅助下的发现式学习模式对中学生物十一年级学生的学习自主性有影响。
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引用次数: 0
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