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Chapter 2 – MALDI-TOF Mass Spectrometry for Microorganism Identification 第2章- MALDI-TOF质谱法用于微生物鉴定
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-12-31 DOI: 10.1016/BS.MIM.2015.07.003
L. Bourassa, S. Butler-Wu
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引用次数: 14
Index 指数
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-11-27 DOI: 10.1016/S0580-9517(15)00029-X
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引用次数: 0
Next-Generation Sequencing for Pathogen Detection and Identification 新一代病原体检测和鉴定测序技术
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.06.004
K. Frey, K. Bishop-Lilly
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引用次数: 14
Gene Amplification and Sequencing for Bacterial Identification 细菌鉴定的基因扩增和测序
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.04.003
S. Lau, J. L. Teng, Chi-chun Ho, P. Woo
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引用次数: 13
Total Laboratory Automation in Clinical Bacteriology 临床细菌学完全实验室自动化
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.09.002
S. Novak-Weekley, E. Marlowe
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引用次数: 0
Host-Based Diagnostics for Detection and Prognosis of Infectious Diseases 基于宿主的传染病检测和预后诊断
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.06.001
William E. Yang, C. Woods, E. Tsalik
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引用次数: 4
Chapter 8 - Technical and Software Advances in Bacterial Pathogen Typing 第八章-细菌病原体分型的技术和软件进展
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.06.003
L. Chui, V. Li
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引用次数: 2
Low-Density TaqMan® Array Cards for the Detection of Pathogens. 用于检测病原体的低密度 TaqMan® 阵列卡。
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 Epub Date: 2015-08-03 DOI: 10.1016/bs.mim.2015.06.002
Jude Heaney, Kathryn Rolfe, Nicholas S Gleadall, Jane S Greatorex, Martin D Curran

Real-time PCR assays have revolutionised diagnostic microbiology over the past 15 years or more. Adaptations and improvements over that time frame have led to the development of multiplex assays. However, limitations in terms of available fluorophores has meant the number of assays which can be combined has remained in single figures. This latter limitation has led to the focus tending to be on individual pathogens and their detection. This chapter describes the development of TaqMan® Array Cards (TACs), technology which allows the detection of multiple pathogens (up to 48 targets) from a single nucleic acid extract, utilising small volumes and real-time PCR. This in turn lends itself to a syndromic approach to infectious disease diagnosis. Using the examples of TACs we have developed in our own laboratory, as well as others, we explain the design, optimisation and use of TACs for respiratory, gastrointestinal and liver infections. Refinement of individual assays is discussed as well as the incorporation of appropriate internal and process controls onto the array cards. Finally, specific examples are given of instances where the assays have had a direct, positive impact on patient care.

在过去的 15 年或更长的时间里，实时 PCR 检测技术彻底改变了微生物诊断技术。随着时间的推移，经过调整和改进，开发出了多重检测方法。然而，由于可用荧光基团的限制，可组合的检测数量仍然是个位数。后一种限制导致人们倾向于关注单个病原体及其检测。本章将介绍 TaqMan® 阵列卡 (TAC) 的开发过程，该技术可利用小容量和实时 PCR 从单个核酸提取物中检测多种病原体（最多 48 个目标）。这反过来又为传染病的综合症诊断提供了可能。我们以自己的实验室和其他实验室开发的 TAC 为例，介绍了针对呼吸道、胃肠道和肝脏感染的 TAC 的设计、优化和使用。我们还讨论了个别检测方法的改进以及在阵列卡中加入适当的内部控制和过程控制的问题。最后，还举例说明了这些检测方法对患者护理产生的直接、积极影响。
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引用次数: 0
Solid and Suspension Microarrays for Microbial Diagnostics. 用于微生物诊断的固体和悬浮微阵列。
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 Epub Date: 2015-05-14 DOI: 10.1016/bs.mim.2015.04.002
Steve Miller, Ulas Karaoz, Eoin Brodie, Sherry Dunbar

Advancements in molecular technologies have provided new platforms that are being increasingly adopted for use in the clinical microbiology laboratory. Among these, microarray methods are particularly well suited for diagnostics as they allow multiplexing, or the ability to test for multiple targets simultaneously from the same specimen. Microarray technologies commonly used for the detection and identification of microbial targets include solid-state microarrays, electronic microarrays and bead suspension microarrays. Microarray methods have been applied to microbial detection, genotyping and antimicrobial resistance gene detection. Microarrays can offer a panel approach to diagnose specific patient presentations, such as respiratory or gastrointestinal infections, and can discriminate isolates by genotype for tracking epidemiology and outbreak investigations. And, as more information has become available on specific genes and pathways involved in antimicrobial resistance, we are beginning to be able to predict susceptibility patterns based on sequence detection for particular organisms. With further advances in automated microarray processing methods and genotype-phenotype prediction algorithms, these tests will become even more useful as an adjunct or replacement for conventional antimicrobial susceptibility testing, allowing for more rapid selection of targeted therapy for infectious diseases.

分子技术的进步提供了新的平台，越来越多地被临床微生物实验室采用。其中，微阵列方法尤其适用于诊断，因为这种方法可以进行多重检测，或从同一标本中同时检测多个目标。常用于检测和鉴定微生物靶标的微阵列技术包括固态微阵列、电子微阵列和微珠悬浮微阵列。微阵列方法已被应用于微生物检测、基因分型和抗菌药耐药基因检测。微阵列可提供一种面板方法，用于诊断特定的病人症状，如呼吸道或胃肠道感染，并可通过基因型区分分离物，用于追踪流行病学和疫情调查。此外，随着涉及抗菌药耐药性的特定基因和途径的信息越来越多，我们开始能够根据特定生物体的序列检测来预测药敏模式。随着自动微阵列处理方法和基因型-表型预测算法的进一步发展，这些检测作为传统抗菌药敏感性检测的辅助手段或替代手段将变得更加有用，从而可以更快地选择针对传染病的疗法。
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引用次数: 0
MALDI-TOF Mass Spectrometry in the Clinical Microbiology Laboratory; Beyond Identification MALDI-TOF质谱法在临床微生物学实验室中的应用除了识别
4区 生物学 Q2 Medicine
Methods in Microbiology
Pub Date : 2015-01-01 DOI: 10.1016/BS.MIM.2015.04.004
S. Schubert, M. Kostrzewa
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引用次数: 8
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