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Bioavailability of Curcumin Encapsulated in Yeast Cells Saccharomyces Cerevisiae 姜黄素包封在酵母细胞中的生物利用度
Pub Date : 2023-09-22 DOI: 10.29141/2500-1922-2023-8-3-10
Irina V. Kalinina, Rinat I. Fatkullin, Natalya V. Naumenko, Natalia V. Popova, Ekaterina E. Naumenko, Darya S. Stepanova
The development of a new food systems generation with the declared functional properties and efficiency is impossible without a scientifically based food system development and test at the preclinical assessment stage. Thus, there is a need in searching simple and inexpensive technological solutions developed to modify biologically active substances in order to deliver it to human body systems efficiently. The research aims at studying the possibility of Saccharomyces cerevisiae yeast cells use to encapsulate the plant antioxidant curcumin and this approach impact on bioavailability and antioxidant properties preservation in an in vitro digestion model. The study used commercially available Saccharomyces cerevisiae yeast; curcumin from Nanjing Duly Biotech Co., Ltd. (China). The researchers encapsulated curcumin in nanostructured form. The nanostructuring process using an immersion-type ultrasonic device enabled changing the dispersed curcumin particles composition and morphology. The encapsulation technology of curcumin in yeast cells included mechanical mixing of components in a ratio of 3:1 (yeast – curcumin) at a temperature of 28 °C for 24 hours. A man monitored the process after 12 and 24 hours; determined the potential bioavailability and bioactivity of curcumin in its initial form and its encapsulated complex using an in vitro digestion model. As a research result, yeast cells are able to act as a “delivery system” of curcumin in the circumstances of its pre-nanostructuring. Potential bioavailability and bioactivity analysis of the plant antioxidant in the in vitro digestion model demonstrated that the encapsulation technology in yeast cells enabled an increase in the bioavailability index of curcumin by 1.34 times, and the bioactivity index by 1.225 times compared to its non-encapsulated form. To obtain a higher level of bioavailability and bioactivity, a man has to develop technologies to increase the curcumin encapsulation efficiency in yeast cells forming the basis of future research.
如果没有基于科学的食品系统开发和临床前评估阶段的测试,开发具有所声明的功能特性和效率的新一代食品系统是不可能的。因此,有必要寻找简单而廉价的技术解决方案来修饰生物活性物质,以便有效地将其输送到人体系统。本研究旨在研究酵母细胞包封植物抗氧化剂姜黄素的可能性及其对体外消化模型生物利用度和抗氧化性能保存的影响。这项研究使用了市售的酿酒酵母;姜黄素,南京德力生物科技有限公司(中国)研究人员将姜黄素封装成纳米结构。采用浸没式超声装置的纳米化工艺可以改变分散的姜黄素颗粒的组成和形态。姜黄素在酵母细胞中的包封技术是将酵母-姜黄素按3:1的比例在28℃下机械混合24小时。一名男子在12小时和24小时后监测这一过程;利用体外消化模型测定姜黄素初始形态及其包封复合物的潜在生物利用度和生物活性。作为一项研究结果,酵母细胞能够在姜黄素纳米结构前的情况下充当姜黄素的“传递系统”。植物抗氧化剂体外消化模型的潜在生物利用度和生物活性分析表明,酵母细胞包封技术使姜黄素的生物利用度指数比未包封的姜黄素提高1.34倍,生物活性指数提高1.225倍。为了获得更高水平的生物利用度和生物活性,人们必须开发技术来提高姜黄素在酵母细胞中的包封效率,这是未来研究的基础。
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引用次数: 0
Lipid Profile Biomarkers Implication in Preschool Organizations Menu Assessment 脂质谱生物标志物在学前组织菜单评估中的意义
Pub Date : 2023-09-22 DOI: 10.29141/2500-1922-2023-8-3-8
Tatiana V. Mazhaeva, Yulia S. Chernova, Aleksey I. Chemezov
The search for nutrition biomarkers, forming the so-called metabotype, is of great importance for assessing the nutrition model and its impact on human health. Currently, there is a large number of studies conducted on the metabolomic diet fat component profile; scientists are studying the acylcarnitines spectrum depending on the diet type, increasingly. Still, there is deficiency of the lipid profile biomarkers importance in the preschool organization menu assessment. The aim of the study was to determine the lipid profile biomarkers importance in assessing the preschool children menu. The researchers evaluated the menu of two preschool organizations of the Sverdlovsk region and analyzed the metabolic profile of 190 children attending it. The greatest excess of saturated and unsaturated fatty acids content, the sources of which were animal products in the menu of one of the preschool organizations, is relatable to a higher content of medium-chain and long-chain acylcarnitines in the children blood serum; in turn, there is a higher content of short-chain acylcarnitines in serum in the course of a higher amount of vegetable fats in the diet. Acylcarnitines can act not only as markers of rational nutrition, but also as risk factors for the development of a whole disease range. Therefore, the data obtained in the study on the acylcarnitine content in children with different amounts of saturated and unsaturated fatty acids in the diet can act as biomarkers of an unbalanced diet for the fat component with subsequent correction.
寻找营养生物标志物,形成所谓的代谢型,对于评估营养模型及其对人类健康的影响具有重要意义。目前,对代谢组学饮食脂肪成分谱进行了大量的研究;科学家们正在越来越多地根据饮食类型研究酰基肉碱谱。然而,脂质谱生物标志物在学前组织菜单评估中的重要性仍存在不足。该研究的目的是确定脂质谱生物标志物在评估学龄前儿童菜单中的重要性。研究人员评估了斯维尔德洛夫斯克地区两家学前组织的菜单,并分析了190名参加该组织的儿童的代谢状况。饱和和不饱和脂肪酸含量的最大过剩与儿童血清中中链和长链酰基肉碱含量较高有关,其中饱和和不饱和脂肪酸的来源是某幼儿园菜单上的动物产品;反过来,随着饲料中植物脂肪含量的增加,血清中短链酰基肉碱的含量也会增加。酰基肉碱不仅可以作为合理营养的标志,而且可以作为整个疾病范围发展的危险因素。因此,在饮食中不同数量的饱和和不饱和脂肪酸的儿童中,研究中获得的酰基肉碱含量数据可以作为脂肪成分不平衡饮食的生物标志物,并进行后续校正。
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引用次数: 1
Thermodynamic Analysis of Static Moisture Sorption Patterns by Ectocarpus Based on Its Hygroscopic Characteristics 基于吸湿特性的外腕树静态吸湿模式热力学分析
Pub Date : 2023-09-22 DOI: 10.29141/2500-1922-2023-8-3-12
Ekaterina Y. Zaporozhets, Ekaterina V. Fomenko, Albert H.-H. Nugmanov, Lina Yu. Lagutkina, Igor Yu. Aleksanyan, Victoria N. Savinova
The most popular biopolymers in the food industry are polysaccharides, including sodium alginate (food additive E401) used as a thickener in the jelly, sauces and fillings production, as well as a moisture-retaining ingredient in bread, bakery products, etc.; and brown algae of marine nature (kelp and fucus) serve as its unique industrial source in modern conditions. Brown algae of the Caspian basin including Ectocarpus are rich of alginate. The research aim is to determine the hygroscopic parameters during moisture sorption by ectocarpus, and conduct their thermodynamic analysis to specify the energy consumption during drying and to identify its rational modes. The scientific analysis object was ectocarpus in native, dry and crushed form. The thesis exemplifies the empirical data on humidity and sorption isotherms for algal raw materials, equilibrium curves constructed in semi-logarithmic coordinates; studies the macrostructural layer tissue organization of brown algae raw materials recovered in an aqueous medium; determines the stages on sorption isotherms and the graphical dependence r = f (Wр). As a research result, a man determined the hygroscopic parameters of algal raw materials during moisture sorption, run a thermodynamic analysis of the obtained sorption isotherms to identify energy costs at various stages of drying, and revealed its rational modes, in which the final result of the drying operation is the production of a material with high biological value and the best parameters for its preservation.
食品工业中最受欢迎的生物聚合物是多糖,包括海藻酸钠(食品添加剂E401),用作果冻、酱汁和馅料生产中的增稠剂,以及面包、烘焙产品等中的保湿成分;在现代条件下,海洋褐藻(海带和墨角藻)是其独特的工业来源。里海盆地的褐藻含有丰富的海藻酸盐。研究目的是确定外腕果吸湿过程中的吸湿参数,并对其进行热力学分析,明确干燥过程中的能量消耗,确定其合理模式。科学分析对象为天然干燥粉碎形态的外腕果。本文举例说明了藻类原料的湿度和吸附等温线的经验数据,在半对数坐标下构建平衡曲线;研究了在水介质中回收的褐藻原料的宏观结构层组织结构。确定了吸附等温线上的阶段和图形依赖性r = f (wr)。作为一项研究成果,一个人确定了藻类原料在吸湿过程中的吸湿参数,对所得的吸湿等温线进行热力学分析,确定了干燥各阶段的能量消耗,并揭示了其合理的模式,其中干燥操作的最终结果是生产出具有高生物价值的材料和最佳的保存参数。
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引用次数: 0
Symbiotic Complex and Secondary Raw Materials Use in the Functional Product Development 共生复合体和二次原料在功能性产品开发中的应用
Pub Date : 2023-09-22 DOI: 10.29141/2500-1922-2023-8-3-4
Vladimir A. Lazarev, Gennady B. Pishchikov, Olga S. Chechenikhina
Nowadays, the sugar substitute use has an application in food more widely due to its advantages, such as low-calorie content, safety, high taste qualities, small portion size. The study aims at recipe development and production technology improvement of marshmallows based on sweeteners enriched with a synbiotic complex. In the developed formulation, a man replaced sugar with Jerusalem artichoke syrup and isomalt, introduced the probiotic Bifidobacterium bifidum in an amount of 5·107 CFU/g. The authors determined the optimal quantitative parameters of sugar replacement with Jerusalem artichoke syrup and isomalt. The research revealed that the humidity of the developed pastille product was 14.6% less than that of the control one, explained by the isomalt low hygroscopicity. The water–soluble vitamins content is, mg/100 g: thiamine – 1.14; riboflavin – 0.34; ascorbic acid – 19.86. The mass fraction of reducing sugars (2.31%) and the mass fraction of sugars (86.24%) are higher than in the control. At the same time, a sample made according to the developed recipe contains 63% lower calories than a sample made according to a standard recipe. Permissible levels of mesophilic aerobic and facultative anaerobic microorganisms of a functional marshmallow sample are constant for 72 hours (no more than 1·103 CFU/g). Despite the decrease in humidity (by 14.6%), the experimental marshmallow sample had high organoleptic indicators – 4.76 points on a five-point scale. The developed pastille product recipe will expand the range of enriched confectionery products for functional purposes.
目前,糖替代品因其热量低、安全、口感好、份量小等优点,在食品中的应用越来越广泛。本研究的目的是研究以合成复合甜味剂为基础的棉花糖的配方开发和生产工艺改进。在开发的配方中,一名男子用耶路撒冷洋蓟糖浆和异麦芽糖代替糖,并引入了两歧双歧杆菌,用量为5·107 CFU/g。确定了用洋蓟糖浆和异麦芽糖替代糖的最佳定量参数。研究表明,研制的颗粒产品的湿度比对照产品低14.6%,这是由于异糖糖的低吸湿性所致。水溶性维生素含量为,mg/ 100g:硫胺素- 1.14;核黄素- 0.34;抗坏血酸- 19.86。还原糖质量分数(2.31%)和糖质量分数(86.24%)均高于对照。与此同时,根据开发的配方制作的样品比根据标准配方制作的样品含有的卡路里低63%。功能棉花糖样品中嗜温好氧和兼性厌氧微生物的允许水平在72小时内保持不变(不超过1.103 CFU/g)。尽管湿度下降了14.6%,但实验棉花糖样品的感官指标很高——在五分制中为4.76分。开发的颗粒产品配方将扩大功能性强化糖果产品的范围。
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引用次数: 0
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