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Effects of partial coconut flour fortification and butter reduction with pumpkinpuree in rice donuts 在米饭甜甜圈中添加部分椰子粉和南瓜蛋白减少黄油用量的效果
Pub Date : 2024-07-06 DOI: 10.26656/fr.2017.8(4).393
A. Akesowan, A. Chareonkul
Coconut flour is rich in dietary fiber and protein, is lower in calories, and has a lovelytropical aroma than rice flour. At the same time, plant-based fat replacers, such as fruitand vegetable puree, which can provide moisture, thickening properties, and lowercalories than fat, are popularly used in low-fat baked products. Thus, a reduced-buttergluten-free rice donut with coconut flour has been developed for people with celiacdisease. This study evaluated the effect of different coconut flour levels (0%, 10%, 20%and 30% (w/w)) for rice flour in donuts under all-butter and reduced-butter (butter / pumpkin puree = 75 /25 w/w) conditions. The experimental results were subjected to a two-way analysis of variance, and principal component analysis was used for perceptualmapping. The physical properties assessed were specific volume, firmness, L*, a*, and b*,while a 9-point hedonic scale was used for sensory evaluation. Incorporating 10% to 30%coconut flour significantly decreased the specific volume but increased firmness, L*, a*,and b*, in all-butter and reduced-butter conditions. Rice donuts with 10% coconut flourreceived higher scores for sensory perception than those made with 20% and 30% coconutflour. Replacing 25% of the butter with pumpkin puree improved some sensory attributessuch as color, flavor, and texture. The optimal formulation was the rice donut fortifiedwith 10% coconut flour in the reduced-butter condition.
与米粉相比,椰子粉富含膳食纤维和蛋白质,热量较低,并具有可爱的热带香味。与此同时,植物性脂肪替代品,如水果和蔬菜泥,可以提供水分、增稠性能和比脂肪更低的热量,被广泛用于低脂烘焙产品中。因此,针对乳糜泻患者开发出了椰子粉无奶油麸质大米甜甜圈。本研究评估了在全黄油和少黄油(黄油/南瓜泥=75/25 w/w)条件下,不同椰子粉含量(0%、10%、20%和 30%(w/w))对甜甜圈米粉的影响。对实验结果进行了双向方差分析,并使用主成分分析法绘制感知图。评估的物理特性包括比容、硬度、L*、a* 和 b*,而感官评估则采用了 9 点享乐量表。在全黄油和少黄油条件下,加入 10%至 30%的椰子粉会显著降低比容,但会增加坚固度、L*、a* 和 b*。与使用 20% 和 30% 椰子粉制作的甜甜圈相比,使用 10% 椰子粉制作的甜甜圈获得了更高的感官评分。用南瓜泥代替 25% 的黄油可改善某些感官属性,如颜色、风味和口感。在减少黄油的条件下,最佳配方是添加了 10%椰子粉的大米甜甜圈。
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引用次数: 0
In vitro evaluation of probiotic potential of Lactobacillus paracasei BIOTECH10363 strain isolated from Tapuy 对从塔布伊分离的副干酪乳杆菌 BIOTECH10363 菌株的益生菌潜力进行体外评估
Pub Date : 2024-07-06 DOI: 10.26656/fr.2017.8(4).385
J. Malilay, J.A.N. Bautista, K. Castillo-Israel, M.C.R. Oliveros
Fermented foods can be a source of potentially probiotic microorganisms as they containdifferent types of lactic acid bacteria. In the Philippines, there are a variety of traditionalfermented food products that may harbor probiotics. This study determined the probioticproperties of Lactobacillus paracasei BIOTECH 10363 isolated from Tapuy, anindigenous alcoholic beverage made from fermented glutinous white rice. The acid andbile resistance, antibacterial, cell surface hydrophobicity, auto-aggregation, NaCltolerance, hemolytic, and antibiotic sensitivity properties of L. paracasei BIOTECH10363 were evaluated in vitro. The isolate displayed resistance to acid (pH 3 to 5) and bile(0.1 to 0.5%). Its cell-free supernatant exhibited strong inhibitory activity againstEscherichia coli. Higher affinity to xylene and chloroform was observed in L. paracaseiBIOTECH 10363. This suggests that the cell surface of the isolate has hydrophobic andelectron donor (basic) properties. Moreover, the isolate had a strong auto-aggregatingphenotype. Cell surface hydrophobicity and strong auto-aggregation activity could enablethe isolate to adhere, colonize and survive in the gastrointestinal tract. The isolate couldwithstand MRS broth with 9% NaCl. It also displayed γ-hemolytic activity in blood agarplates indicating the non-hemolytic nature of the isolate. L. paracasei BIOTECH 10363expressed sensitivity against various cell wall synthesis inhibitors (amoxicillin, ampicillin,augmentin, penicillin, and cefaclor) and protein synthesis inhibitors (streptomycin,chloramphenicol, clindamycin, erythromycin, and tetracycline). However, it displayedintrinsic resistance toward kanamycin. The findings of this study showed the promisingpotential of L. paracasei BIOTECH 10363 as a candidate probiotic strain as it exhibiteddesirable attributes in vitro.
发酵食品可能是益生微生物的来源,因为它们含有不同类型的乳酸菌。菲律宾有多种可能含有益生菌的传统发酵食品。本研究测定了从 Tapuy(一种由发酵糯米制成的土著酒精饮料)中分离出来的副干酪乳杆菌 BIOTECH 10363 的益生菌特性。在体外评估了帕拉卡西乳杆菌 BIOTECH10363 的耐酸和耐碱性、抗菌性、细胞表面疏水性、自动聚集性、耐氯化钠性、溶血性和抗生素敏感性。该分离菌株对酸(pH 3 至 5)和胆汁(0.1 至 0.5%)具有抗性。其无细胞上清液对大肠杆菌有很强的抑制活性。副卡西氏菌生物技术 10363 对二甲苯和氯仿具有更高的亲和力。这表明该分离物的细胞表面具有疏水性和电子供体(碱性)特性。此外,该分离物具有很强的自动聚集表型。细胞表面的疏水性和强大的自动聚集活性可使该分离物在胃肠道中附着、定殖和存活。该分离物能耐受含 9% NaCl 的 MRS 肉汤。它还在血液琼脂平板中显示出γ-溶血活性,表明该分离物不溶血。L. paracasei BIOTECH 10363 对多种细胞壁合成抑制剂(阿莫西林、氨苄西林、安体舒通、青霉素和头孢克洛)和蛋白质合成抑制剂(链霉素、氯霉素、克林霉素、红霉素和四环素)表现出敏感性。不过,它对卡那霉素表现出内在抗性。这项研究的结果表明,帕拉卡氏乳杆菌 BIOTECH 10363 在体外表现出了理想的特性,有望成为候选益生菌株。
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引用次数: 0
The impact of food hygiene and sanitation on food quality in public and privateelementary school canteens 食品卫生和环境卫生对公立和私立小学食堂食品质量的影响
Pub Date : 2024-07-06 DOI: 10.26656/fr.2017.8(4).237
R. Ningsih, R. Murwani, M.I. Kartasurya, Sulistiyani
Cases of food poisoning after consuming snacks, food, and drinks from elementary schoolcanteens are still widely occurring in the Central Java Province of Indonesia. This studyaimed to evaluate food hygiene sanitation and food quality in public and privateelementary school canteens. This study was an observational study with a cross-sectionaldesign. The sample was 225 school canteens selected by stratified random samplingtechniques. Hygiene and sanitation data were obtained using an observational checklistfrom the Indonesian Health Ministry and laboratory tests. Physical observations andlaboratory tests were used to examine the food quality in school canteens and the canteenpremises. The data were analyzed using the chi-square and Fisher’s exact test. The resultsshowed that the quality of school canteen food that did not comply with the six criteria offood hygiene and sanitation was 0.88%, 7.1% in chemical tests, and 44% inmicrobiological tests. It also revealed differences in the food quality in the chemicaltesting in public and private school canteens (p = 0.039). There was a relationship betweenfood quality, food raw materials (p = 0.022), and the kitchens where the food is prepared(p = 0.006). There were differences in the physical test results between food raw materialsand food quality in public and private primary school canteens (p = 0.034). The findingsconfirmed that hygiene and sanitation have an impact on food quality. Chemical foodcontamination was introduced to the food through cheap, poor-quality additives andpreservatives, which are banned for use in the food industry. This study highlights theneed for regular implementation, guidance, and enforcement of food safety checks inschool canteens, emphasizing food hygiene and sanitation to prevent deliberate foodcontamination and cross-contamination from the surrounding environment, storage, andhandling.
在印度尼西亚中爪哇省,因食用小学食堂的零食、食物和饮料而导致食物中毒的病例仍在广泛发生。本研究旨在评估公立和私立小学食堂的食品卫生和食品质量。本研究是一项横断面设计的观察性研究。样本是通过分层随机抽样技术选出的 225 个学校食堂。卫生和环境卫生数据是通过印尼卫生部提供的观察清单和实验室测试获得的。物理观察和实验室测试用于检查学校食堂和食堂设施的食品质量。数据采用卡方检验和费雪精确检验进行分析。结果显示,学校食堂食品质量不符合食品卫生和环境卫生六项标准的比例为 0.88%,化学检测不符合标准的比例为 7.1%,微生物检测不符合标准的比例为 44%。调查还显示,公立和私立学校食堂在化学检测中的食品质量存在差异(p = 0.039)。食品质量与食品原材料(p = 0.022)和制作食品的厨房(p = 0.006)之间存在关系。公立和私立小学食堂的食品原材料与食品质量之间的物理检测结果存在差异(p = 0.034)。研究结果证实,卫生和环境卫生对食品质量有影响。化学食品污染是通过廉价、劣质的添加剂和防腐剂引入食品的,而这些添加剂和防腐剂已被禁止在食品工业中使用。这项研究强调,有必要在学校食堂定期实施、指导和执行食品安全检查,强调食品卫生和环境卫生,以防止蓄意的食品污染和来自周围环境、储存和处理的交叉污染。
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引用次数: 0
Effect of carrot pulp on the physicochemical, microbiological and sensoryattributes of kulfi 胡萝卜果肉对库尔菲的理化、微生物和感官属性的影响
Pub Date : 2024-07-03 DOI: 10.26656/fr.2017.8(4).213
M.J. Akhter, M. I. Kabir, M. Sohany, M.H. Islam, A.A. Khatun, A. Hosen, M.F. Kabir
Modified traditional food items with healthier additives have attracted researchers for theirnutritional benefits and enhanced tastes. The present study aimed to prepare traditionalkulfi blended with carrot pulp and to investigate the effect of added carrot on thephysicochemical, microbiological and sensory qualities of kulfi. The cost analysis of kulfiwas also conducted. A total of four samples (T1, T2, T3 and T4) were prepared byincorporating 0%, 5%, 10% and 15% carrot pulp, respectively, where T1 (0% carrot pulp)was the control sample. The results demonstrated that the moisture content and fibreincreased while fat, protein, total soluble solid, carbohydrate, and ash in the kulfi samplesdecreased with the increasing amount of carrot pulp. The pH value was higher in thecarrot pulp with the addition of kulfi than in the control one. However, the melting rate ofdifferent treatments decreased with the addition of carrot pulp. For all sensorycharacteristics, the highest value was obtained by T3 (10% carrot pulp) among the othertreatments. The microbial counts in all treated samples were at safe levels, and there wasno presence of yeast, mold and coliforms. The cost structure of the product revealed areduction in the production cost for carrot pulp-supplemented kulfi compared to thecontrol kulfi. It was calculated that the average production cost of each kulfi was 32.90BDT (0.38 USD), 32.40 BDT (0.38 USD), 31.90 BDT (0.37 USD), and 31.40 BDT (0.37USD) for sample T1, T2, T3 and T4, respectively, which appeared to be reasonablycompetitive. In this research, the partial incorporation of carrot pulp resulted in a safe, low-cost, and consumer-based kulfi with variations in its physicochemical attributes.
使用更健康的添加剂对传统食品进行改良后,其营养价值和口感都吸引了研究人员。本研究旨在制备掺入胡萝卜浆的传统库尔菲,并调查添加胡萝卜对库尔菲的理化、微生物和感官质量的影响。此外,还对库尔菲进行了成本分析。通过分别加入 0%、5%、10% 和 15%的胡萝卜浆,共制备了四种样品(T1、T2、T3 和 T4),其中 T1(0% 胡萝卜浆)为对照样品。结果表明,随着胡萝卜果肉含量的增加,库尔菲样品中的水分含量和纤维增加,而脂肪、蛋白质、总可溶性固体、碳水化合物和灰分则减少。添加了库尔菲的胡萝卜果肉的 pH 值高于对照组。然而,不同处理的熔化率随着胡萝卜果肉的添加而降低。在所有感官特征中,T3(10% 胡萝卜果肉)在其他处理中获得的数值最高。所有处理过的样品中的微生物数量都处于安全水平,没有酵母、霉菌和大肠菌群。产品的成本结构显示,与对照库尔菲相比,添加胡萝卜浆的库尔菲的生产成本有所降低。根据计算,样品 T1、T2、T3 和 T4 的平均生产成本分别为 32.90BDT (0.38 美元)、32.40 BDT (0.38 美元)、31.90 BDT (0.37 美元)和 31.40 BDT (0.37 美元),似乎具有合理的竞争力。在这项研究中,胡萝卜果肉的部分加入使库尔菲变得安全、低成本,并具有不同的理化属性。
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引用次数: 0
Effects of genetic disclosure based on gene polymorphisms in TAS1R2(rs12033832) and UCP-2 (rs659366) on sugar restriction 基于 TAS1R2(rs12033832)和 UCP-2 (rs659366)基因多态性的遗传信息披露对糖限制的影响
Pub Date : 2024-07-03 DOI: 10.26656/fr.2017.8(4).240
R.A. Saputri, R.D. Muliadi, D. Subali, F. Kartawidjajaputra, A. Suwanto
High sugar intake is one of the factors that may contribute to rising obesity and type 2 diabetes, hence changing one’s lifestyle is necessary. It is known that single nucleotide polymorphism in TAS1R2 (rs12033832) associates with one’s sugar intake, whereas rs659366 in UCP-2 associates with diabetes risk. The purpose of this study was to evaluate the effects of genetic disclosure based on rs12033832 and rs659366 in health programs to the sugar restriction. Participants (n = 41) were Indonesians who underwent a 14-day health transformation program in which they were divided into two groups, control (without genetic disclosure) and genetic disclosure (GD) group. It was observed that both groups significantly reduced their sugar intake (g/day), in the control group (49.75±19.18 vs 32.04±20.14; p-value = 0.009) and the GD group (49.1±18.48 vs 33.92±13.93; p-value = 0.002). Moreover, participants who possessed a high risk of type 2 diabetes mellitus (T2DM) were shown to significantly reduce their sugar intake (48±21.39 vs 31.06±12.12; p-value = 0.003), whereas the decrease in the low-risk group was not significant. The proportion of participants who exceed daily dose recommendation (>50 g/day) decreased as well from 50% to 38% (p = 0.047) in the control group, whilst the GD group decreased from 38% to 9.5% (p = 0.030). These findings demonstrated that there were positive impacts from the program regarding the sugar restriction regardless of genetic disclosure. Nevertheless, health programs with genetic disclosure could be more valuable than general health programs.
高糖摄入量是导致肥胖和 2 型糖尿病增加的因素之一,因此改变生活方式很有必要。众所周知,TAS1R2 的单核苷酸多态性(rs12033832)与人的糖摄入量有关,而 UCP-2 的 rs659366 与糖尿病风险有关。本研究的目的是评估基于 rs12033832 和 rs659366 的基因披露在健康计划中对糖限制的影响。参与者(n = 41)均为印尼人,他们接受了为期 14 天的健康转型计划,并被分为两组,即对照组(无基因公开)和基因公开(GD)组。据观察,两组的糖摄入量(克/天)都明显减少,对照组(49.75±19.18 vs 32.04±20.14;P 值 = 0.009)和基因公开组(49.1±18.48 vs 33.92±13.93;P 值 = 0.002)。此外,2 型糖尿病(T2DM)高风险参与者的糖摄入量明显减少(48±21.39 vs 31.06±12.12;p 值 = 0.003),而低风险组的糖摄入量减少并不明显。对照组中超过每日推荐剂量(>50 克/天)的参与者比例也从 50% 降至 38%(p = 0.047),而广东组则从 38% 降至 9.5%(p = 0.030)。这些研究结果表明,无论基因披露与否,该计划都对糖限制产生了积极影响。尽管如此,披露基因的健康计划可能比一般的健康计划更有价值。
{"title":"Effects of genetic disclosure based on gene polymorphisms in TAS1R2\u0000(rs12033832) and UCP-2 (rs659366) on sugar restriction","authors":"R.A. Saputri, R.D. Muliadi, D. Subali, F. Kartawidjajaputra, A. Suwanto","doi":"10.26656/fr.2017.8(4).240","DOIUrl":"https://doi.org/10.26656/fr.2017.8(4).240","url":null,"abstract":"High sugar intake is one of the factors that may contribute to rising obesity and type 2 diabetes, hence changing one’s lifestyle is necessary. It is known that single nucleotide polymorphism in TAS1R2 (rs12033832) associates with one’s sugar intake, whereas rs659366 in UCP-2 associates with diabetes risk. The purpose of this study was to evaluate the effects of genetic disclosure based on rs12033832 and rs659366 in health programs to the sugar restriction. Participants (n = 41) were Indonesians who underwent a 14-day health transformation program in which they were divided into two groups, control (without genetic disclosure) and genetic disclosure (GD) group. It was observed that both groups significantly reduced their sugar intake (g/day), in the control group (49.75±19.18 vs 32.04±20.14; p-value = 0.009) and the GD group (49.1±18.48 vs 33.92±13.93; p-value = 0.002). Moreover, participants who possessed a high risk of type 2 diabetes mellitus (T2DM) were shown to significantly reduce their sugar intake (48±21.39 vs 31.06±12.12; p-value = 0.003), whereas the decrease in the low-risk group was not significant. The proportion of participants who exceed daily dose recommendation (>50 g/day) decreased as well from 50% to 38% (p = 0.047) in the control group, whilst the GD group decreased from 38% to 9.5% (p = 0.030). These findings demonstrated that there were positive impacts from the program regarding the sugar restriction regardless of genetic disclosure. Nevertheless, health programs with genetic disclosure could be more valuable than general health programs.","PeriodicalId":502485,"journal":{"name":"Food Research","volume":"74 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141684005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glucomannan powder can be used as a food substitute for people with diabetesmellitus 葡甘露聚糖粉可作为糖尿病患者的代餐食品
Pub Date : 2024-07-03 DOI: 10.26656/fr.2017.8(4).361
S. Sudjarwo, R. Rofiq, P.A. Eka, P.F.A. Maria, A.M. Faris, R. Widyowati
Glucomannan contained in porang tuber (Amorphophallus oncophyllus) includespolysaccharide fiber which can be used as an alternative food replacement for diet,especially in treating diabetes. It contains calcium oxalate and long-term use can causeharmful effects. Six male mice in each dose group, aged 2-2.5 months, and bodyweight 20-40 g were used in this study. Mice were induced by alloxan monohydrate to producediabetic mice. The test group was mice given porang powder standardized byglucomannan with a dose of 85, 165 and 250 mg/kg BW mice for 21 days and glucosemeasurements were taken every three days. A toxicity study with a randomized postcontrol group design used six mice in the dose group. The number of deaths wascalculated with three kinds of methods (Weil method, Line method and Probit method) toproduce the LD50 of porang powder. The data were then analyzed using One Way Anovawith a significant value of 5%. Porang powder based on glucomannan with a dose of 250mg/kg BW mice reduced glucose levels significantly after 13 days of administration(p<0.05). The highest dose (5000 mg/kg BW mice) of porang powder standardized byglucomannan for 48 hrs did not show death in mice and was not toxic.
茯苓块茎(Amorphophallus oncophyllus)所含的葡甘露聚糖包括多糖纤维,可作为饮食的替代食物,尤其是在治疗糖尿病方面。它含有草酸钙,长期服用会产生有害影响。本研究使用 2-2.5 月龄、体重 20-40 克的雄性小鼠,每个剂量组 6 只。用一水阿脲诱导小鼠产生糖尿病。试验组小鼠连续 21 天分别服用 85、165 和 250 毫克/千克体重的葡甘露聚糖标准化猪苓粉,每三天测量一次血糖。毒性研究采用随机后对照组设计,剂量组使用六只小鼠。用三种方法(Weil 法、Line 法和 Probit 法)计算死亡数,得出茯苓粉的半数致死剂量。然后用单向分析法对数据进行分析,显著值为 5%。剂量为 250 毫克/千克体重的葡甘露聚糖茯苓粉在给小鼠服用 13 天后可显著降低血糖水平(p<0.05)。最高剂量(5000 毫克/千克体重小鼠)的葡甘聚糖标准化茯苓粉持续服用 48 小时后,小鼠没有死亡,没有毒性。
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引用次数: 0
Passion fruit juice concentration using fabricated cellulose triacetate/celluloseacetate forward osmosis membrane 使用三醋酸纤维素/醋酸纤维素正向渗透膜浓缩百香果汁
Pub Date : 2024-07-03 DOI: 10.26656/fr.2017.8(4).540
A.I. Mohamad Idris, S. M. Mustapa Kamal, A. Sulaiman, R. Omar, M. Mohammad
The concentration of juice using an evaporator is an energy-intensive process. Forwardosmosis membrane can be used as an alternative for juice concentration at significantlylow energy. In this work, fabricated cellulose triacetate (CTA)/cellulose acetate (CA)forward osmosis membrane was applied to concentrate passion fruit juice. The feasibilityof the fabricated CTA/CA membrane on concentrating the passion fruit juice wasdetermined. The FO membrane was prepared by fabricating the mixture of CTA/CA withthe inclusion of polyvinylpyrrolidone (PVP) as an additive. The CTA/CA forward osmosismembrane was characterized and compared with the fabricated pure CA membrane (withthe inclusion of PVP). The synthesized CTA/CA membrane was shown to have a highporosity, desired hydrophilicity, and a reduced impact of ICP, which generally affectedthe forward osmosis process. The concentration of passion fruit juice was successfullyperformed at an average of 2.2 LMH of water flux. The latter showed that the fabricatedCTA/CA forward osmosis membrane has the potential to concentrate the passion fruitjuice. It can be further improved by increasing the concentration of the draw solution(NaCl) or using another type of draw solution that has higher osmotic pressure.
使用蒸发器浓缩果汁是一个能源密集型过程。正向渗透膜可作为浓缩果汁的替代方法,而且能耗低得多。在这项工作中,制作的三醋酸纤维素(CTA)/醋酸纤维素(CA)前向渗透膜被用于浓缩百香果汁。确定了制作的 CTA/CA 膜浓缩百香果汁的可行性。FO 膜是通过制造 CTA/CA 混合物并加入聚乙烯吡咯烷酮(PVP)作为添加剂制备的。对 CTA/CA 正向渗透膜进行了表征,并与制造的纯 CA 膜(含有 PVP)进行了比较。结果表明,合成的 CTA/CA 膜具有较高的孔隙率和理想的亲水性,并减少了通常影响正向渗透过程的 ICP 的影响。以平均 2.2 LMH 的水通量成功浓缩了百香果汁。后者表明所制造的 CTA/CA 正渗透膜具有浓缩百香果汁的潜力。可以通过提高汲取液(NaCl)的浓度或使用渗透压更高的其他类型汲取液来进一步改进。
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引用次数: 0
Analysis of lard in cod liver oil emulsion using FTIR spectroscopy combinedprincipal component analysis 利用傅立叶变换红外光谱联合主要成分分析法分析鱼肝油乳液中的猪油
Pub Date : 2024-06-16 DOI: 10.26656/fr.2017.8(3).329
Zilhadia Zilhadia, Y. Anggraeni, Y.F. Apriyanti, M. Mustafidah, I. Jaswir
Cod liver oil (CLO) contains essential fatty acids and has a high price in the market.Therefore, CLO can be targeted for adulteration to increase economic profits. One of thesubstances used for adulteration of CLO is lard. This study aimed to analyze the spectralprofile of lard and CLO in cod liver oil emulsion using Fourier transform infrared (FTIR)spectroscopy combined with principal component analysis (PCA). Lard and CLO weremixed with a range of concentrations of 0-100% used as the control. CLO emulsions weremade with a composition of lard and CLO in concentrations similar to those of the control.To analyze the lard and CLO spectrum, lard and CLO were extracted from the emulsionusing liquid-liquid extraction, and then the oil extracted from the emulsion was analyzedusing FTIR spectroscopy and PCA. Fourier transform infrared spectrum absorption atregion 1117–1098 cm-1 is specific to identifying the different spectrums of CLO and lard.The results of PCA showed that clustering between similar oil content and a combinationof FTIR and PCA is effective in distinguishing the spectrum of lard and CLO in cod liveroil emulsion.
鳕鱼肝油(CLO)含有人体必需的脂肪酸,在市场上价格不菲。猪油是掺假 CLO 的物质之一。本研究旨在利用傅立叶变换红外光谱法(FTIR)结合主成分分析法(PCA)分析鱼肝油乳液中猪油和 CLO 的光谱特征。将猪油和 CLO 混合,以 0-100% 的浓度范围作为对照。为了分析猪油和 CLO 的光谱,使用液-液萃取法从乳液中提取猪油和 CLO,然后使用傅立叶变换红外光谱和主成分分析法对从乳液中提取的油进行分析。傅立叶变换红外光谱在 1117-1098 cm-1 区域的吸收对识别 CLO 和猪油的不同光谱具有特异性,PCA 的结果表明,相似油含量之间的聚类以及傅立叶变换红外光谱和 PCA 的组合能有效区分鳕鱼肝油乳液中猪油和 CLO 的光谱。
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引用次数: 0
Physicochemical and antibacterial properties of chitosan extracted fromswimming crab shells and wooden grasshoppers using different extractionmethods 用不同提取方法从螃蟹壳和木蚱蜢中提取壳聚糖的理化性质和抗菌特性
Pub Date : 2024-06-16 DOI: 10.26656/fr.2017.8(3).313
A. S. Anggraeni, A. Jayanegara, E. B. Laconi, N. R. Kumalasari, A. Windarsih, A. Sofyan
Marine by-products and insects are among the sources of chitin used in chitosanproduction and increase the value of the product that may be used in the food industry.The conversion of chitin to chitosan requires proper extraction methods in order tominimise energy use and waste while also producing good-quality chitosan. This studyaimed to evaluate different methods of extracting chitosan from two sources and tocharacterise its physicochemical and antibacterial properties. The study utilised twodistinct chitosan sources, i.e. crab shells and wooden grasshoppers, as well as two distinctextraction methods, i.e. conventional and green chemistry methods. The yield, water-ashcontent, solubility, physicochemical properties as determined by infrared spectroscopy(FTIR), degree of deacetylation (DD), crystallinity (XRD), microstructure (SEM) andantibacterial activity were all evaluated for chitosan quality. The results indicated that thegreen chemistry extraction of crab shells (M2P1 treatment) produced the highest yield,solubility and crystallinity index of all treatments, with a DD of 60.9%. The functionalgroups and microstructure of chitosan were remarkably similar across all treatments.Antibacterial activity was determined using the microdilution method against Grampositive (Clostridium acetobutylicum) and Gram-negative (Escherichia coli) bacteria andthe minimum inhibitory concentrations were identified, notably 2000 ppm for the greenchemistry method. The green chemical extraction method using crab shells (M2P1)demonstrated that the extracted chitosan possessed beneficial physicochemical properties,especially on yield and solubility, and antimicrobial properties against both Gram-positiveand Gram-negative bacteria. As such, based on the DD percentage and antibacterialactivity, this implies that the extracted chitosan may be used as an alternative for thepreservation of food in the food industry.
海洋副产品和昆虫是壳聚糖生产中使用的甲壳素来源之一,可提高产品在食品工业中的使用价值。将甲壳素转化为壳聚糖需要适当的提取方法,以便在生产优质壳聚糖的同时最大限度地减少能源消耗和浪费。本研究旨在评估从两种来源中提取壳聚糖的不同方法,并描述其物理化学和抗菌特性。研究利用了两种不同的壳聚糖来源,即蟹壳和木质蚱蜢,以及两种不同的提取方法,即传统方法和绿色化学方法。对壳聚糖的产率、水灰含量、溶解度、红外光谱(FTIR)测定的理化性质、脱乙酰度(DD)、结晶度(XRD)、显微结构(SEM)和抗菌活性进行了质量评价。结果表明,在所有处理方法中,蟹壳的绿色化学提取(M2P1 处理)产生的产率、溶解度和结晶度指数最高,DD 为 60.9%。采用微量稀释法测定了壳聚糖对革兰氏阳性菌(乙酰丁酸梭菌)和革兰氏阴性菌(大肠杆菌)的抗菌活性,并确定了最小抑菌浓度,其中绿色化学法的最小抑菌浓度为 2000 ppm。使用蟹壳的绿色化学提取法(M2P1)表明,提取的壳聚糖具有良好的理化特性,特别是在产量和溶解度方面,而且对革兰氏阳性菌和革兰氏阴性菌都具有抗菌特性。因此,根据 DD 百分比和抗菌活性,这意味着提取的壳聚糖可用作食品工业中保存食品的替代品。
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引用次数: 0
The use of real-time polymerase chain reaction for detection of raw rat meat(Rattus norvegicus) with species-specific primer for halal authentication 使用实时聚合酶链式反应检测生大鼠肉(Rattus norvegicus),并使用物种特异性引物进行清真认证
Pub Date : 2024-06-16 DOI: 10.26656/fr.2017.8(3).486
D. Lestari, Wirnawati, H. Hamzah, K.S.I. Kurniasih, D. Hamidi, S. Syofyan, A. Rohman
Rat meat (RM) can be obtained freely from farmers and can be used as a potentialadulterant in meat-based food products. The presence of RM in any food products isprohibited by the Muslim community and is considered non-halal meat. Therefore, ananalytical method capable of analysing RM specifically is very urgent. In this study, a real-time polymerase chain reaction using a species-specific primer targeting NADHdehydrogenase subunit 6 (ND6) is applied to analyze RM. DNA extraction was carried outusing FavorPrepTM Tissue Genomic DNA Extraction Mini Kit, and the extracted DNAwas subjected to purity index using NANO-Quant SPARK TECAN. The annealingtemperature (Ta) used for PCR analysis was optimized to get the best Ta capable ofproviding the optimum amplification reaction. Furthermore, some performancecharacteristics were evaluated for Real-Time PCR, including sensitivity, efficiency andrepeatability. The results exhibited that the designed primer is specific to rat DNAextracted toward other DNAs from meats typically used in food products. For quantitativeevaluation, the limit of detection found was 0.39 ng with an efficiency of amplification (E)of 94.4% with a coefficient of determination (R2) of 0.986 for the relationship between logconcentrations of DNA and cycle threshold (Ct) values. The repeatability assay wasacceptable with an RSD value ≤ 25%. The developed method was reliable, provided highsensitivity for the analysis of RM. Therefore, this method is suitable to be used as astandard method for halal authentication analysis.
鼠肉(RM)可以从农民手中随意获得,可用作肉类食品的潜在掺杂剂。穆斯林社区禁止在任何食品中使用鼠肉,并将其视为非清真肉类。因此,迫切需要一种能够专门分析 RM 的分析方法。在本研究中,使用针对 NADHdehydrogenase subunit 6 (ND6) 的物种特异性引物进行实时聚合酶链反应来分析 RM。DNA 提取采用 FavorPrepTM 组织基因组 DNA 提取迷你试剂盒,提取的 DNA 采用 NANO-Quant SPARK TECAN 进行纯度检测。对用于 PCR 分析的退火温度(Ta)进行了优化,以获得能产生最佳扩增反应的最佳 Ta。此外,还评估了 Real-Time PCR 的一些性能特征,包括灵敏度、效率和重复性。结果表明,所设计的引物对大鼠 DNA 具有特异性,对食品中典型肉类中提取的其他 DNA 也具有特异性。在定量评估方面,检测限为 0.39 纳克,扩增效率(E)为 94.4%,DNA 的对数浓度与周期阈值(Ct)之间的决定系数(R2)为 0.986。重复性测定结果合格,RSD 值小于 25%。该方法可靠、灵敏度高,可用于RM的分析。因此,该方法适合作为清真食品认证分析的标准方法。
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Food Research
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