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Effects of processing conditions on change of amino acids, reducing sugar andtotal polyphenols of caramelized malt produced from IR50404 rice variety 加工条件对 IR50404 稻种焦糖麦芽氨基酸、还原糖和总多酚变化的影响
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(2).982
T.H. Nguyen, C. Nguyen
Rice beer is a value-added product from rice since it consists of enough conditions formaking beer. To produce the best caramelized malt from IR50404 rice variety,understanding the effects of processing conditions on change of amino acids, reducingsugar and total polyphenols is very important for developing such rice beer processing.Changes in chemical compositions and enzyme activities in caramelized malt processedfrom IR50404 rice variety were understood. Suitable germination conditions wereindicated, including a soaking temperature of 30oC for 24 hrs, degree of steeping of 35-36% and germination time of 4 days at 30oC. Green rice malt was incubated at 40-70oCfor 30-120 mins. Then, rice malt was roasted at different temperatures of 125-200oC for30-90 mins. The results showed that both tempering and roasting processes changed theconcentration of polyphenols, reducing sugars and amino acids. When green malt wasincubated at 50°C for 60 mins, reducing sugars, amino acids and polyphenol content(TPC) increased by 2 times (102.72 mg/g), 1.8 times (9.08 mg/g) and 1.5 times (4.26 mgGAE/g) respectively corresponding to the improvement of the amylase and proteaseactivity. Moreover, there were maximum increases in phenolic compounds in roasted maltat 125 and 150°C for 45 and 75 mins for tempered malt and green malt, respectively. Incontrast, higher heat treatment (roasted at 175-200°C) resulted in reducing the levels of allevaluated variables. Thus, malting with a moderate thermal treatment (125-150°C) couldbe considered an effective process to enrich (or enhance) antioxidants in rice grains fortheir further use as a functional ingredient in malt and beer production.
大米啤酒是大米的高附加值产品,因为它具备足够的啤酒酿造条件。为了从 IR50404 水稻品种中生产出最佳的焦糖麦芽,了解加工条件对氨基酸、还原糖和总多酚变化的影响对开发此类米啤加工非常重要。确定了合适的发芽条件,包括 30 摄氏度浸泡 24 小时,浸泡度为 35-36%,在 30 摄氏度下发芽 4 天。绿色稻芽在 40-70oC 温度下培养 30-120 分钟。然后在 125-200oC 的不同温度下焙烧 30-90 分钟。结果表明,调质和焙烧过程都会改变多酚、还原糖和氨基酸的浓度。绿麦芽在 50°C 下培养 60 分钟后,还原糖、氨基酸和多酚含量(TPC)分别增加了 2 倍(102.72 mg/g)、1.8 倍(9.08 mg/g)和 1.5 倍(4.26 mgGAE/g),与淀粉酶和蛋白酶活性的提高相对应。此外,调质麦芽和绿色麦芽分别在 125 和 150°C 煅烧 45 和 75 分钟后,酚类化合物的增幅最大。相反,更高的热处理温度(175-200°C 的焙烧温度)会降低所评估变量的水平。因此,采用适度热处理(125-150°C)进行麦芽加工可被视为一种有效的工艺,可丰富(或提高)米粒中的抗氧化剂,使其进一步用作麦芽和啤酒生产中的功能性配料。
{"title":"Effects of processing conditions on change of amino acids, reducing sugar and\u0000total polyphenols of caramelized malt produced from IR50404 rice variety","authors":"T.H. Nguyen, C. Nguyen","doi":"10.26656/fr.2017.8(2).982","DOIUrl":"https://doi.org/10.26656/fr.2017.8(2).982","url":null,"abstract":"Rice beer is a value-added product from rice since it consists of enough conditions for\u0000making beer. To produce the best caramelized malt from IR50404 rice variety,\u0000understanding the effects of processing conditions on change of amino acids, reducing\u0000sugar and total polyphenols is very important for developing such rice beer processing.\u0000Changes in chemical compositions and enzyme activities in caramelized malt processed\u0000from IR50404 rice variety were understood. Suitable germination conditions were\u0000indicated, including a soaking temperature of 30oC for 24 hrs, degree of steeping of 35-\u000036% and germination time of 4 days at 30oC. Green rice malt was incubated at 40-70oC\u0000for 30-120 mins. Then, rice malt was roasted at different temperatures of 125-200oC for\u000030-90 mins. The results showed that both tempering and roasting processes changed the\u0000concentration of polyphenols, reducing sugars and amino acids. When green malt was\u0000incubated at 50°C for 60 mins, reducing sugars, amino acids and polyphenol content\u0000(TPC) increased by 2 times (102.72 mg/g), 1.8 times (9.08 mg/g) and 1.5 times (4.26 mg\u0000GAE/g) respectively corresponding to the improvement of the amylase and protease\u0000activity. Moreover, there were maximum increases in phenolic compounds in roasted malt\u0000at 125 and 150°C for 45 and 75 mins for tempered malt and green malt, respectively. In\u0000contrast, higher heat treatment (roasted at 175-200°C) resulted in reducing the levels of all\u0000evaluated variables. Thus, malting with a moderate thermal treatment (125-150°C) could\u0000be considered an effective process to enrich (or enhance) antioxidants in rice grains for\u0000their further use as a functional ingredient in malt and beer production.","PeriodicalId":502485,"journal":{"name":"Food Research","volume":"50 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140363186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The changes in the physical and chemical quality of catfish oil (Pangasius sp.)with rice bran oil addition during room temperature storage 添加米糠油的鲶鱼油在常温贮藏过程中理化品质的变化
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(s2).28
D. Widowati, Siswanti, R. Anandito, L. Purnamayati
Catfish oil (CFO) is an oil product that can be obtained through catfish extraction by thedry rendering method and followed by a purification procedure. Fish oil has a high contentof unsaturated fatty acid and is susceptible to oxidation and hydrolysis processes leadingto a decrease in physical and chemical quality. Rice bran oil (RBO) which is rich inphenolic acid has a good antioxidant potential to inhibit the deterioration of fish oil duringstorage. This study aimed to determine the effect of the CFO and the RBO ratio on thephysical and chemical quality of the CFO with the RBO addition during room temperaturestorage. This research used a completely randomized design with one factor. Data wereanalyzed by one-way analysis of variance method and continued by Duncan’s MultipleRange Tests (p<0.05). The ratio of CFO:RBO was 100:0, 90:10, 80:20, 70:30 and 60:40.Data were taken at storage times of weeks 0, 2, 4 and 6. Based on the results obtained, theratio of 60(CFO):40(RBO) at week 0 has the best physical and chemical quality. It can beconcluded that the addition of RBO on CFO effectively inhibits the hydrolysis andoxidation damage up to 6 weeks of room temperature storage.
鲶鱼油(CFO)是一种油产品,可通过干渲染法提取鲶鱼,然后进行净化处理。鱼油不饱和脂肪酸含量高,易受氧化和水解过程的影响,导致物理和化学质量下降。米糠油(RBO)富含酚酸,具有良好的抗氧化潜力,可抑制鱼油在储存过程中的变质。本研究旨在确定 CFO 和 RBO 的比例对添加了 RBO 的 CFO 在室温储存期间的物理和化学质量的影响。本研究采用了一个因素的完全随机设计。数据采用单因素方差分析法进行分析,并继续进行邓肯多重范围检验(P<0.05)。CFO 与 RBO 的比例分别为 100:0、90:10、80:20、70:30 和 60:40。根据所得结果,在第 0 周时,60(CFO):40(RBO)的比例具有最佳的物理和化学质量。由此可以得出结论,在 CFO 中添加 RBO 能有效抑制水解和氧化破坏,室温储存 6 周后效果最佳。
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引用次数: 0
Non-destructive determination of water content in fruits using Vis-NIRspectroscopy 利用可见光-近红外光谱法无损测定水果中的水分含量
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(s2).14
H. Z. Amanah, E. Pratiwi, D. N. Rahmi, M. Pahlawan, R. Masithoh
Visible near-infrared (Vis-NIR) spectroscopy is less accurate for detecting -OHmolecules, therefore, the use of Vis-NIR spectroscopy is challenging for samplescontaining high water content, such as fruits. However, as Vis-NIR spectroscopy is aportable and economical instrument, it can be used in the field by small-scale farmers.This study aimed to evaluate Vis-NIR spectra to measure water content in fruits. Fourfruits were used in this study, including dragon fruit, guava, sapodilla and banana (100pieces each). All fruits were randomly divided into a calibration set (two-thirds of thesamples) and a prediction set (one-third of the samples). Water content was predictedusing partial least square regression (PLSR) analysis. The PLSR calibration model had acoefficient of determination (R²) of 0.29 for dragon fruit, 0.63 for guava, 0.62 forsapodilla and 0.80 for banana. The prediction model had an R² of 0.11 for dragon fruit,0.63 for guava, 0.52 for sapodilla and 0.75 for banana. These results show that Vis-NIRspectroscopy has the potential to predict water content in relatively low water-contentfruits, such as bananas.
可见光近红外光谱(Vis-NIR)检测-OH 分子的准确性较低,因此,对于水果等含水量较高的样品来说,使用可见光近红外光谱具有挑战性。然而,由于可见光-近红外光谱仪是一种便携且经济的仪器,小规模农户可以在田间使用该仪器。本研究使用了四种水果,包括火龙果、番石榴、无患子和香蕉(各 100 件)。所有水果被随机分为校准集(三分之二的样品)和预测集(三分之一的样品)。含水量通过偏最小二乘法回归(PLSR)分析进行预测。偏最小二乘法回归校准模型的判定系数(R²)分别为:火龙果 0.29,番石榴 0.63,山榄 0.62,香蕉 0.80。火龙果、番石榴和香蕉预测模型的 R² 分别为 0.11、0.63、0.52 和 0.75。这些结果表明,可见光-近红外光谱法有可能预测香蕉等含水量相对较低的水果的含水量。
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引用次数: 0
The effect of alginate extract on the properties of brown rice (Oryza nivara) icecream 海藻酸提取物对糙米冰淇淋特性的影响
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(s2).47
R.B. Nuary, L. Maghfira, D. P. Suleman, A. N. Rochmah, F. Zulfa, R.B.K. Anandita
The production of ice cream with the basic ingredients of brown rice milk is carried out toincrease the consumption of antioxidants derived from brown rice (Oryza nivara). This isbecause the plant is less consumed due to its rough texture. The recent development ofbrown rice ice cream (BRICE) is intended to expand the use of the plant into a commoncommodity. Previous investigations have established that stabilizers are required in icecream production, although only a few used natural stabilizers such as alginate. Therefore,this study aimed to determine the effect of adding the different concentrations of alginatefrom brown seaweed Laminaria sp. on the physical characteristic, namely melting power,viscosity, overrun, total dissolved solids, chemical properties, which include protein, fat,as well as antioxidant activity and hedonic level of BRICE. The concentration of alginateused was at 0.025%, 0.05%, 0.075%, and 0.1%, while the data collected were analyzedusing analysis of variance (ANOVA) and Duncan’s test. The result showed that theaddition of different alginate concentrations had a significant effect on the value of theparameters observed.
用糙米牛奶的基本成分制作冰淇淋,是为了增加从糙米(Oryza nivara)中提取的抗氧化剂的消费量。这是因为糙米质地粗糙,消费量较少。最近开发的糙米冰淇淋(BRICE)旨在扩大糙米的使用范围,使其成为一种普通商品。以往的研究表明,冰淇淋生产需要稳定剂,但只有少数研究使用了海藻酸盐等天然稳定剂。因此,本研究旨在确定添加不同浓度的褐海藻海藻酸盐对冰激凌的物理特性(即熔化力、粘度、溢出、总溶解固体)、化学特性(包括蛋白质、脂肪)以及抗氧化活性和享乐水平的影响。使用的海藻酸浓度分别为 0.025%、0.05%、0.075% 和 0.1%,收集的数据通过方差分析(ANOVA)和邓肯检验进行分析。结果表明,添加不同浓度的海藻酸盐对观察到的参数值有显著影响。
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引用次数: 0
Cookies of purple sweet potato and tempeh flour as functional foods: nutritionalvalue and sensory acceptability 作为功能性食品的紫甘薯和豆豉粉饼干:营养价值和感官可接受性
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(s2).53
F. Mushollini, H. Salimo, L.R. Kartikasari
Purple sweet potato is a local food that entails health functions for the human body. Theuse of purple sweet potato in the production of cookies provides adequate and potentialnutrition as a functional food. As a source of carbohydrates, purple sweet potato isenriched with vitamins and minerals such as calcium, iron, and antioxidants and displays aunique colour. The addition of protein source food ingredients, tempeh flour, for instance,is essential to complement the nutritional value of cookies’ protein. This study aimed toanalyse the nutritional value and sensory acceptability on cookies of purple sweet potatoand tempeh flour. Cookies were tested proximately and for sensory acceptability (colour,flavour, texture, taste and overall parameters) with the ratios of purple sweet potato flourand tempeh flour, comprising 100:1 (FC01), 95:5 (FC02), 90:10 (FC03), 85:15 (FC04),and 80:20 (FC05). The results showed that cookies had moisture (2.05-4.28%), ash (1.05-4.18%), protein (8.06-22.31%), fat (4.19-7.61%), and carbohydrate (63.85-82.42%)content. The result of sensory acceptability showed that there was a significant differenceon the aroma of the cookies by adding purple sweet potato flour and tempeh flour.Tempeh flour can increase the protein content of cookies. The most preferred cookies isgroup of cookies with treatment FC02, had 80% of purple sweet potato flour and 20%tempeh flour.
紫甘薯是一种对人体具有保健功能的当地食品。紫甘薯作为一种功能性食品,可为饼干生产提供充足和潜在的营养。作为碳水化合物的来源,紫甘薯富含维生素和矿物质,如钙、铁和抗氧化剂,并呈现出独特的颜色。添加蛋白质来源的食品配料,例如豆豉粉,对于补充饼干蛋白质的营养价值至关重要。本研究旨在分析紫甘薯和豆豉粉饼干的营养价值和感官可接受性。对紫甘薯粉和豆豉粉的比例(100:1(FC01)、95:5(FC02)、90:10(FC03)、85:15(FC04)和 80:20(FC05))进行了饼干近似性和感官可接受性(色泽、风味、质地、口感和总体参数)测试。结果表明,饼干的水分(2.05%-4.28%)、灰分(1.05%-4.18%)、蛋白质(8.06%-22.31%)、脂肪(4.19%-7.61%)和碳水化合物(63.85%-82.42%)含量。感官可接受性结果表明,添加紫甘薯粉和豆豉粉后,饼干的香味有显著差异。最受欢迎的饼干是处理 FC02 的饼干组,其中紫甘薯粉占 80%,豆豉粉占 20%。
{"title":"Cookies of purple sweet potato and tempeh flour as functional foods: nutritional\u0000value and sensory acceptability","authors":"F. Mushollini, H. Salimo, L.R. Kartikasari","doi":"10.26656/fr.2017.8(s2).53","DOIUrl":"https://doi.org/10.26656/fr.2017.8(s2).53","url":null,"abstract":"Purple sweet potato is a local food that entails health functions for the human body. The\u0000use of purple sweet potato in the production of cookies provides adequate and potential\u0000nutrition as a functional food. As a source of carbohydrates, purple sweet potato is\u0000enriched with vitamins and minerals such as calcium, iron, and antioxidants and displays a\u0000unique colour. The addition of protein source food ingredients, tempeh flour, for instance,\u0000is essential to complement the nutritional value of cookies’ protein. This study aimed to\u0000analyse the nutritional value and sensory acceptability on cookies of purple sweet potato\u0000and tempeh flour. Cookies were tested proximately and for sensory acceptability (colour,\u0000flavour, texture, taste and overall parameters) with the ratios of purple sweet potato flour\u0000and tempeh flour, comprising 100:1 (FC01), 95:5 (FC02), 90:10 (FC03), 85:15 (FC04),\u0000and 80:20 (FC05). The results showed that cookies had moisture (2.05-4.28%), ash (1.05-\u00004.18%), protein (8.06-22.31%), fat (4.19-7.61%), and carbohydrate (63.85-82.42%)\u0000content. The result of sensory acceptability showed that there was a significant difference\u0000on the aroma of the cookies by adding purple sweet potato flour and tempeh flour.\u0000Tempeh flour can increase the protein content of cookies. The most preferred cookies is\u0000group of cookies with treatment FC02, had 80% of purple sweet potato flour and 20%\u0000tempeh flour.","PeriodicalId":502485,"journal":{"name":"Food Research","volume":"29 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140362254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of different plant parts and solvents on bioactive compounds andantioxidation in large fruit Bird’s eye chili (Capsicum annuum L. cv. Superhot) 不同植物部分和溶剂对大果雀眼辣椒(Capsicum annuum L. cv. Superhot)中生物活性化合物和抗氧化性的影响
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(2).621
P. Khanema, A. Srisuwan, J. Manasathien
The production chain is impacted by an overabundance of large fruit Bird’s eye chili(Capsicum annuum L. cv. Superhot) on the market. Another way to make the most of thepotential of raw materials for product development is to supplement the data onphytochemicals and antioxidative activity by segmentation (fruit, placenta, seed, andpericarp). In an experiment, distilled water, 60% ethanol and 90% ethanol were used asthe primary solvents. The outcomes demonstrated that placenta and seeds using 90%ethanol as a solvent (PS-Et90) contained capsaicin, total phenolics, and total flavonoids attheir most outstanding levels (16.30 µg/mg, 44.65 µg GAE/mg, and 21.77 µg CE/mg,respectively). At the same time, the Scoville Heat Unit was high at 244,550 SHU (veryhigh pungency). Additionally, it demonstrated the highest antioxidative activity (129.57µg TE/mg) when tested with the ABTS method, and total flavonoids were discovered toenhance the antioxidation effect when measured by ABTS and FRAP methods. PS-Et90had a significantly different potency than the other groups when the extracts were groupedusing Agglomerative Hierarchical Cluster Analysis based on phytochemical data andantioxidant activity. Therefore, the placenta and seeds appear to be preferable over otherswhen utilizing large fruit Bird’s eye chili for pharmacological purposes. Using 90%ethanol as a solvent will provide the best bioavailability.
生产链受到市场上大果雀眼辣椒(Capsicum annuum L. cv. Superhot)过多的影响。另一种充分利用原材料潜力进行产品开发的方法是通过细分(果实、胎盘、种子和果皮)来补充有关植物化学物质和抗氧化活性的数据。在一项实验中,蒸馏水、60% 乙醇和 90% 乙醇被用作主要溶剂。结果表明,以 90% 乙醇为溶剂的胎盘和种子(PS-Et90)中辣椒素、总酚类和总黄酮类化合物的含量最高(分别为 16.30 µg/mg、44.65 µg GAE/mg 和 21.77 µg CE/mg)。同时,斯科维尔热量单位高达 244550 SHU(辛辣度极高)。此外,用 ABTS 法检测,它的抗氧化活性最高(129.57µg TE/mg),用 ABTS 法和 FRAP 法检测,发现总黄酮类化合物增强了抗氧化效果。根据植物化学数据和抗氧化活性,采用聚类分层聚类分析法对提取物进行分组时,PS-Et90 的功效与其他组有明显差异。因此,在将大果雀舌辣椒用于药理目的时,胎盘和种子似乎比其他成分更可取。使用 90% 的乙醇作为溶剂将提供最佳的生物利用度。
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引用次数: 0
Investigation of the sensory quality, nutritional value and antioxidant capacityof flakes prepared using various pigeon pea-based flours 使用各种鸽豌豆基面粉制备薄片的感官质量、营养价值和抗氧化能力的研究
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(s2).18
S. Ariviani, G.P. Nastiti
Pigeon pea (Cajanus cajan (L.)) is a potential source of nutritional and antioxidantcompounds. Germination proved to improve the nutritional quality and antioxidantcapacity of pigeon peas. The previous author’s study showed that pigeon pea flourprepared by NaCl elicitation before germination exhibits significantly higher antioxidantcapacity and functional properties than that prepared without elicitation or germination.The study aimed to examine the sensory quality, nutrition value, and antioxidant capacityof flakes formulated using non-germinated pigeon pea flour, pigeon pea sprout flour, andNaCl-elicited pigeon pea sprout flour. The potential of the flours to be developed ascommercial flakes was also determined using oat-based commercial flakes as acomparator. The NaCl-elicited pigeon pea flour-based flakes showed a higher qualityscore of both texture and overall qualities than the other pigeon pea flour-based flakes andthe commercial ones. Regarding nutritional value, flakes formulated using NaCl-elicitedpigeon pea sprout flour also showed better nutritional value, indicated by the lowest fatcontent and highest soluble, insoluble, and total dietary fiber contents. The highest valuesof total phenolics content (TPC), Trolox equivalent antioxidant capacity (TEAC), andferric reducing antioxidant power (FRAP) were also observed in the NaCl-elicited pigeonpea sprout flour-based flakes, even though its DPPH radical scavenging activity was notsignificantly different to the commercial flakes. These results have significantconsequences for developing legume-based flakes with higher levels of dietary fibers andantioxidant potential, and lower fat content.
豌豆(Cajanus cajan (L.))是一种潜在的营养和抗氧化化合物来源。事实证明,发芽能提高鸽子豆的营养质量和抗氧化能力。作者的研究表明,在豌豆发芽前用氯化钠激发法制备的豌豆粉,其抗氧化能力和功能特性明显高于未激发或未发芽的豌豆粉。此外,还以基于燕麦的商用薄片为参照物,测定了这些面粉作为商用薄片的开发潜力。NaCl引发的鸽子豆粉片在质地和整体质量方面的得分都高于其他鸽子豆粉片和商品片。在营养价值方面,用 NaCl 引发的鸽子豆芽粉配制的薄片也显示出更好的营养价值,脂肪含量最低,可溶性、不可溶性和总膳食纤维含量最高。尽管 NaCl 引发的鸽子豆芽粉的 DPPH 自由基清除活性与商品粉相比没有显著差异,但总酚类物质含量(TPC)、Trolox 当量抗氧化能力(TEAC)和铁还原抗氧化能力(FRAP)的值也最高。这些结果对于开发膳食纤维含量更高、抗氧化潜力更强、脂肪含量更低的豆类薄片具有重要意义。
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引用次数: 0
Optimization of various solvents for the extraction of anthocyanins from‘sapinit’ (Philippine wild raspberry) using response surface methodology 利用响应面方法优化从 "sapinit"(菲律宾野生树莓)中提取花青素的各种溶剂
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(2).194
J. P. Rivadeneira, M. Ilano, M.B.M. Salvalosa, R.J.D. Recaido, A.T.L. Matro, K. Castillo-Israel, J.P. Tamayo
‘Sapinit,’ officially known as the Philippine wild raspberry, is a promising source ofnatural colourants such as anthocyanins. In this study, extraction of anthocyanins from‘sapinit’ using three solvent systems, namely, acidified acetone, acidified ethanol andacidified methanol were evaluated and optimized. The optimized extraction conditionswere: 4°C, 150 mins, and 50% solid-to-liquid ratio (SLR) for the acidified acetone solventsystem; 110°C, 10 mins, and 60% SLR for the acidified ethanol solvent system; and 26°C,90 min, and 24% SLR for the acidified methanol system. These optimum conditionsresulted in extracts with total monomeric anthocyanin content (TMA) of 540 mg cyanidin3-glucoside/L, 622 mg cyanidin-3-glucoside/L, and 89 mg cyanidin-3-glucoside/L,respectively. Validation procedures showed the generated models were able to predict theoptimum TMA, with at most a 7% difference from the actual values. Moreover, acidifiedethanol was found to be the most appropriate solvent for the extraction of anthocyaninsfrom ‘sapinit.’
'Sapinit',正式名称为菲律宾野生覆盆子,是花青素等天然色素的重要来源。本研究评估并优化了使用酸化丙酮、酸化乙醇和酸化甲醇三种溶剂系统从'sapinit'中提取花青素的方法。优化的萃取条件为酸化丙酮溶剂系统的最佳萃取条件为:4°C,150 分钟,固液比(SLR)50%;酸化乙醇溶剂系统的最佳萃取条件为:110°C,10 分钟,固液比(SLR)60%;酸化甲醇系统的最佳萃取条件为:26°C,90 分钟,固液比(SLR)24%。在这些最佳条件下,提取物中单体花青素总含量(TMA)分别为 540 mg cyanidin-3-glucoside/L、622 mg cyanidin-3-glucoside/L 和 89 mg cyanidin-3-glucoside/L。验证程序表明,生成的模型能够预测出最佳的 TMA,与实际值最多相差 7%。此外,还发现酸化乙醇是萃取无患子花青素最合适的溶剂。
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引用次数: 0
The cytotoxicity of 1,4-bis(3,4,5-trimethoxyphenyl)-tetrahydro-furo(3,4-c) furanisolated from Swietenia macrophylla King seed extract on 3T3-L1 andRAW 264.7 cells 从大叶女贞王种子提取物中分离出的 1,4-双(3,4,5-三甲氧基苯基)-四氢-呋喃(3,4-c)对 3T3-L1 和RAW 264.7 细胞的细胞毒性
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(s2).7
R. D. Yudhani, E. N. Sholikhah, D.A.A. Nugrahaningsih, Mustofa
The exploration of natural products as anti-obesity candidates needs to be performedurgently since obesity has become a worldwide epidemic and many drugs for this healthcondition were withdrawn due to their serious adverse events such as psychiatric disordersand drug dependence. The 1,4-bis(3,4,5-trimethoxyphenyl)-tetrahydro-furo(3,4-c) furan, asaponin isolated from Indonesian Swietenia macrophylla seeds extract has the potential tomodulate obesity because it acts as a potent PPAR-γ agonist in silico and the seeds’hypolipidemic effect has been proven. The cytotoxicity study is a crucial part of the earlypharmaceutical development stage, whilst, the exploration of 1,4-bis(3,4,5-trimethoxyphenyl)-tetrahydro-furo(3,4-c) furan to ensure the compound’s safety in preadipocytes and macrophage cells which play a crucial role in obesity pathogenesis is stilllimited. This study aims to explore the cytotoxicity of saponin isolate on 3T3-L1 (preadipocytes) and RAW 264.7 (macrophages) cell lines to ensure its safety profile in vitro.Both cell lines were exposed to various concentrations of the isolate (3.125-200 µg/mL),then metformin was used as the standard drug and DMSO as solvent control for 24 hrs.The cytotoxicity test was conducted by MTT assay and IC50 was determined by probitanalysis. Most of the 1,4-bis(3,4,5-trimethoxyphenyl)-tetrahydro-furo(3,4-c) furan did notaffect the viability of 3T3-L1 and RAW 264.7 with the IC50 148.90±12.22 and 84.78±1.69µg/mL, respectively. Metformin and DMSO also did not alter the viability of both cells.These results were promising as a basis for further development since the isolates’ IC50value was more than the cytotoxic threshold of NCI guidelines.
肥胖症已成为一种世界性流行病,许多治疗肥胖症的药物因其严重的不良反应(如精神障碍和药物依赖性)而被撤消,因此有必要对作为抗肥胖症候选药物的天然产物进行深入研究。从印尼獐牙菜种子提取物中分离出的 1,4-双(3,4,5-三甲氧基苯基)-四氢-呋喃(3,4-c)皂苷具有调节肥胖症的潜力,因为它是一种有效的 PPAR-γ 激动剂,而且种子的降血脂作用已得到证实。细胞毒性研究是药物早期开发阶段的关键部分,而 1,4-双(3,4,5-三甲氧基苯基)-四氢-呋喃(3,4-c)呋喃在肥胖症发病机制中起关键作用的前脂肪细胞和巨噬细胞中的安全性研究还很有限。本研究旨在探讨皂苷分离物对 3T3-L1(前脂肪细胞)和 RAW 264.7(巨噬细胞)细胞系的细胞毒性,以确保其在体外的安全性。两种细胞系均暴露于不同浓度的皂苷分离物(3.125-200 µg/mL)中,然后以二甲双胍作为标准药物,以二甲基亚砜作为溶剂对照,暴露 24 小时,采用 MTT 法进行细胞毒性试验,并通过概率分析确定 IC50。大多数 1,4-双(3,4,5-三甲氧基苯基)-四氢-呋喃并不影响 3T3-L1 和 RAW 264.7 的活力,IC50 分别为 148.90±12.22 和 84.78±1.69µg/mL。由于分离物的 IC50 值超过了 NCI 指南规定的细胞毒性阈值,因此这些结果有望为进一步开发奠定基础。
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引用次数: 0
Bioprospecting of Vernonia cinerea for nutraceutical and homemade first aidremedies: a review update Vernonia cinerea 用作营养保健品和自制急救药品的生物勘探:最新综述
Pub Date : 2024-03-30 DOI: 10.26656/fr.2017.8(2).227
A. Fadhlina, F. A. Abdul Majid, S.N. Zainol, H.F. Ismail, N.A.N. John Ismail
Vernonia cinerea (VC) is a ubiquitous weed in tropical and subtropical regions. It wastraditionally used to treat health issues by various cultures, but today little is known to thenew generation about its high medicinal values, how to use it as a quick first aid, and itscommercialization potential. A literature search found that VC has been extracted usingvarious kinds of solvents for different polarities of phytochemical extraction.Interestingly, the food-grade solvents such as ethanol and water were found to be suitableto extract the active ingredients from VC for major therapeutic applications such asantioxidants, anti-diabetic, anti-inflammatory, anti-cancer, and anti-microbial as well asbeing a wound healer, hepato-protective, and ease the smoking withdrawals symptoms.The spray-dried water extracts are currently innovated into different forms ofnutraceutical products. The high toxicity threshold allows the utilisation of VC as homeremedies in the form of fresh, dried, and water extracts for oral intake, while the extractsusing common alcohol/spirit and oil can be made for topical applications. This reviewaimed to give an insight into the commercialization potential of VC as a nutraceuticalproduct and the reestablishment of homemade first-aid remedies for various healthconditions especially among the people in rural areas.
Vernonia cinerea(VC)是热带和亚热带地区无处不在的杂草。传统上,各种文化都用它来治疗健康问题,但如今,新一代对它的高药用价值、如何将它用作快速急救以及它的商业化潜力知之甚少。文献检索发现,人们使用各种溶剂提取 VC,以提取不同极性的植物化学物质。有趣的是,人们发现乙醇和水等食品级溶剂适合从 VC 中提取有效成分,用于抗氧化、抗糖尿病、抗炎、抗癌、抗微生物、伤口愈合、保护肝脏和缓解戒烟症状等主要治疗用途。目前,喷雾干燥的水提取物被创新为不同形式的保健品。由于毒性阈值较高,因此可以将新鲜、干燥和水提取物形式的 VC 用作家庭疗法,供口服,而使用普通酒精/烈酒和油制成的提取物则可用于局部应用。本综述旨在深入探讨薇甘菊作为一种营养保健产品的商业化潜力,以及针对各种健康状况(尤其是农村地区的人们)重建自制急救疗法。
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