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Synergistic and antagonistic effects of vinegar and sodium bicarbonate on antibiotic-resistant Escherichia coli 醋和碳酸氢钠对耐抗生素大肠杆菌的协同和拮抗作用
Pub Date : 2024-03-28 DOI: 10.4103/abhs.abhs_82_23
F. Al-Khikani, Hassan Tahir Fareeq, Hassan Abbas Mohammed, Baraa Waleed Jassem, Baraa Wissam Abdul-Hussein, Aya Abdul-Kadhim Banay, Baneen Abd-Ali Jawad, Tabarak Hakeem Hamza, Balqees Malik Hilal
Escherichia coli with antimicrobial resistance has become a frequent global problem in recent decades, which makes it challenging to treat with conventional medicines. This study aimed to evaluate different strategies for managing microbial resistance. E. coli was isolated from wounds and the urinary tract infections and then cultured on blood agar, MacConkey agar and EMB media. The well diffusion method was used to evaluate the activity of vinegar (5%) alone and combined with three concentrations (5%, 10%, and 20%) of sodium bicarbonate (SBC). The diameter of the zone of inhibition was measured in millimeters. Statistical analyses were performed using Excel and the SPSS 26 software. Of the 24 E. coli isolates, approximately 66.6% were sensitive to vinegar combined with 5% SBC compared to 75% sensitivity to vinegar alone. Furthermore, 41.66% of the bacteria were sensitive to vinegar combined with 10% SBC. This study showed that 33.33% of the bacteria were sensitive to vinegar combined with 20% SBC. Comparison of the mean of the inhibition zone for vinegar 13 ± 6.04 mm alone with 20% SBC plus vinegar 7.83 ± 5.56 mm was significant (P = 0.01). Vinegar combined with 10% SBC had an inhibitory effect (antagonism) on the antibacterial activity of vinegar (P = 0.02). The antibacterial efficacy of vinegar with SBC decreased with increasing SBC concentrations, demonstrating an antagonistic effect owing to sodium acetate formation.
近几十年来,对抗生素产生抗药性的大肠埃希菌已成为一个频发的全球性问题,这使得用常规药物治疗具有挑战性。本研究旨在评估管理微生物耐药性的不同策略。 研究人员从伤口和尿路感染处分离出大肠杆菌,然后在血琼脂、麦康凯琼脂和 EMB 培养基上进行培养。采用井扩散法评估醋(5%)单独和与三种浓度(5%、10% 和 20%)的碳酸氢钠(SBC)混合后的活性。抑制区的直径以毫米为单位。统计分析使用 Excel 和 SPSS 26 软件进行。 在 24 个大肠杆菌分离物中,约有 66.6% 对结合了 5% SBC 的食醋敏感,而对单独食醋的敏感度为 75%。此外,41.66%的细菌对醋和 10%的 SBC 混合液敏感。这项研究表明,33.33% 的细菌对醋与 20% SBC 混合使用敏感。单独使用食醋的抑菌区平均值为 13 ± 6.04 mm,而 20% SBC 加食醋的抑菌区平均值为 7.83 ± 5.56 mm,两者相比差异显著(P = 0.01)。醋与 10% SBC 的结合对醋的抗菌活性有抑制作用(拮抗作用)(P = 0.02)。 醋与 SBC 的抗菌效果随着 SBC 浓度的增加而降低,这表明醋酸钠的形成产生了拮抗作用。
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引用次数: 0
Hydropneumothorax as a rare manifestation of primary pulmonary hydatid disease 作为原发性肺水肿病罕见表现的水肺气胸
Pub Date : 2024-03-28 DOI: 10.4103/abhs.abhs_85_23
Mamoun Mohamed Ahmed Ali, Rajesh Gupta, Mohamed Mustafa, Imad Hamad, Devdutt Nayak, Alhaitham Omar, Ahmed El-Barkouky
Echinococcosis, alternatively referred to as hydatid disease, is caused by the larvae of the tapeworm Echinococcus. The primary agents of human infections are Echinococcus granulosus and Echinococcus multilocularis. The present case report pertains to an individual who relocated from an endemic region to the United Arab Emirates and subsequently manifested symptoms of hydropneumothorax. The 44-year-old male patient presented with a 4-day fever and right-sided chest pain, accompanied by cough and breathlessness for 2 weeks. Computed tomography (CT) of the chest revealed right hydropneumothorax and a ruptured hydatid cyst with consolidation. The patient was diagnosed with a ruptured pulmonary hydatid cyst based on a strongly positive hydatid serology and the presence of hydatid tissue on histopathological examination. The patient underwent surgical decortication with cyst removal and received medical treatment with ceftriaxone and albendazole. He was discharged in good condition after a 10-day hospitalisation period. Echinococcosis is a parasitic disease that most often affects the liver and lungs. Although more than half of the patients do not show any symptoms, the main symptoms observed during presentation include dry cough, haemoptysis, and chest pain. Plain radiographs or chest CT scans are used for diagnosis. Surgery is considered the primary treatment for pulmonary hydatid cysts and medical treatment with benzimidazoles (albendazole and mebendazole) is recommended. Complicated pulmonary hydatid disease can manifest in various ways, including hydropneumothorax, as observed in the present case. Early diagnosis, surgical intervention combined with medical treatment, and multidisciplinary teamwork can significantly improve patient outcomes.
棘球蚴病又称包虫病,是由棘球绦虫的幼虫引起的。人类感染的主要病原体是颗粒棘球蚴和多角棘球蚴。本病例报告涉及一名从流行地区搬迁到阿拉伯联合酋长国的患者,他随后出现了水肺气胸的症状。这名 44 岁的男性患者发烧 4 天,右侧胸痛,伴有咳嗽和呼吸困难,持续 2 周。胸部计算机断层扫描(CT)显示患者右侧有水肺气肿和一个破裂的水瘤囊肿,并伴有合并症。根据水包虫血清学强阳性和组织病理学检查发现的水包虫组织,患者被诊断为肺水包虫囊肿破裂。患者接受了囊肿切除手术,并接受了头孢曲松和阿苯达唑药物治疗。住院 10 天后,患者康复出院。棘球蚴病是一种寄生虫病,多发于肝脏和肺部。虽然半数以上的患者没有任何症状,但就诊时观察到的主要症状包括干咳、咯血和胸痛。平片或胸部 CT 扫描可用于诊断。手术被认为是肺包虫囊肿的主要治疗方法,建议使用苯并咪唑类药物(阿苯达唑和甲苯达唑)进行药物治疗。并发症肺包虫病有多种表现形式,包括本病例中观察到的水肺气胸。早期诊断、手术干预与药物治疗相结合以及多学科团队合作可显著改善患者的预后。
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引用次数: 0
Review of occupational exposure to severe acute respiratory syndrome coronavirus-2 in wastewater and its implications in the gulf region 废水中严重急性呼吸系统综合征冠状病毒-2 职业接触及其对海湾地区的影响回顾
Pub Date : 2024-02-21 DOI: 10.4103/abhs.abhs_38_23
Rami Elhadi, Anthony Okoh, Lucy Semerjian
Coronavirus disease-19 (COVID-19) is a viral disease caused by severe acute respiratory syndrome corona virus-2(SARS-CoV-2) that affects the respiratory system and causes varying symptoms including fever and cough. Throughout the current COVID-19 pandemic, several studies have confirmed that raw wastewater may contain the SARS-CoV-2 genome as the virus is shed from excreta of infected individuals. This review examined the literature from 2020 to 2023 on occupational health, safety, wastewater, and SARS-CoV-2, excluding clinical aspects. Relevant publications from previous epidemics (SARS-CoV-1 and Middle East respiratory syndrome coronavirus) were also considered. Quantitative microbial risk assessments have been used previously and in the current pandemic to assess the risk of inhaling aerosolized SARS-CoV-2 generated during the wastewater treatment processes. Meanwhile, water-based epidemiology (WBE) has been effective in identifying and tracking high incidence hotspots as well as mitigating the spread of the disease. Inefficient processes in wastewater treatment plants may be a source of SARS-CoV-2 transmission to workers employed at such plants as well as to personnel handling treated wastewater for reuse purposes such as irrigation and recreation, more so in the gulf countries owing to the extreme limitation of freshwater in this region; thus, exploring the implications of SARS-CoV-2 genome in wastewater to safeguard occupational and public health is crucial. WBE can assist in the pandemic prevention and control measures by investigating SARS-CoV-2 virus survival and transmission through the wastewater treatment and effective control measures. This review presents an overview of occupational exposure to SARS-CoV-2 from wastewater aerosols and elucidates its implications, while recommending preventive and mitigation measures.
冠状病毒病-19(COVID-19)是一种由严重急性呼吸系统综合症冠状病毒-2(SARS-CoV-2)引起的病毒性疾病,会影响呼吸系统并导致发烧和咳嗽等不同症状。在当前 COVID-19 大流行的整个过程中,一些研究证实,未处理的废水中可能含有 SARS-CoV-2 基因组,因为病毒是从受感染者的排泄物中脱落的。本综述审查了 2020 年至 2023 年有关职业健康、安全、废水和 SARS-CoV-2 的文献,但不包括临床方面的文献。还考虑了以往流行病(SARS-CoV-1 和中东呼吸综合征冠状病毒)的相关出版物。定量微生物风险评估曾被用于评估吸入废水处理过程中产生的气溶胶状 SARS-CoV-2 的风险,在本次疫情中也被用于评估吸入气溶胶状 SARS-CoV-2 的风险。同时,以水为基础的流行病学(WBE)在确定和跟踪高发病热点以及减轻疾病传播方面也很有效。在海湾国家,由于淡水资源极度匮乏,废水处理厂的低效流程可能成为 SARS-CoV-2 的传播源,从而传播给受雇于这些处理厂的工人以及处理过的废水用于灌溉和娱乐等再利用目的的人员;因此,探索废水中的 SARS-CoV-2 基因组对保障职业健康和公众健康的影响至关重要。通过调查 SARS-CoV-2 病毒在废水处理过程中的存活和传播情况以及采取有效的控制措施,世界生物多样性基金会可以协助采取大流行病预防和控制措施。本综述概述了废水气溶胶中的 SARS-CoV-2 职业暴露,阐明了其影响,并提出了预防和缓解措施。
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引用次数: 0
Strategies for humanizing technology in health profession education 在卫生专业教育中实现技术人性化的策略
Pub Date : 2024-02-21 DOI: 10.4103/abhs.abhs_90_23
Muhamad Saiful Bahri Yusoff
Technology is rapidly advancing and becoming increasingly critical in the health profession education. Therefore, it is crucial to prioritize the humanization of its implementation to provide students with a personalized, meaningful, and supportive learning experience. Various strategies can be employed to achieve this goal, including humanizing learning institutions, leveraging the urbanization of higher education, revitalizing educational ecosystems, and preparing health-care learners for a changing world. In addition, it is essential to understand the needs of students, use technology as a complementary tool, foster a sense of community, provide training and support, and consider accessibility to ensure the equitable and effective implementation of these strategies. By collaboratively working to humanize technology in health profession education, we can provide students with the education they deserve to excel in their careers.
技术发展日新月异,在卫生专业教育中变得越来越重要。因此,必须优先考虑人性化的实施,为学生提供个性化、有意义和支持性的学习体验。为实现这一目标,可以采取各种策略,包括使学习机构人性化、利用高等教育的城市化、振兴教育生态系统以及使医疗保健学习者为不断变化的世界做好准备。此外,还必须了解学生的需求,将技术作为辅助工具,培养社区意识,提供培训和支持,并考虑无障碍环境,以确保公平有效地实施这些战略。通过共同努力,在卫生专业教育中实现技术的人性化,我们就能为学生提供应有的教育,使他们在职业生涯中取得优异成绩。
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引用次数: 0
Heat-induced changes in the histomorphometry of human femur bone 热引起的人类股骨组织形态学变化
Pub Date : 2024-01-12 DOI: 10.4103/abhs.abhs_67_23
L. Oghenemavwe, C. Orupabo, Shaka Ogheneochuko Praise
Skeletal remains are often subjected to unexpected and adverse conditions which can eventually distort evidence. Therefore, understanding the factors that alter skeletal remains is crucial. A total of human femur fragments obtained from the gross anatomy laboratory of the University of Port Harcourt were used. Bone samples were divided into four groups. Group 1 is the control sample comprising bone fragments not exposed to heat, Group 2 had bones exposed to heat at 20°C, Group 3 had bones exposed to heat at 100°C, and Group 4 had bones exposed to heat at 200°C. Ground bone sections were prepared using Modified Frost’s manual method of bone preparation. Morphometric analysis of the micrographs was performed using the ImageJ software. Data were analyzed using the SPSS software. The mean values of the Haversian canal area and diameter demonstrated a nonsignificant increase in the bone samples exposed to heat compared to the samples not exposed to heat. The osteon count was slightly higher, but the difference was not statistically significant (P > 0.05). The results demonstrated that histomorphometric features of bones are not altered at the temperature of 200°C. This information is useful in forensic investigations.
骸骨遗骸经常会受到意想不到的不利条件的影响,最终导致证据失真。因此,了解改变骨骼遗骸的因素至关重要。 本研究使用了从哈科特港大学大体解剖实验室获得的人类股骨碎片。骨骼样本被分为四组。第 1 组为对照样本,包括未受热的骨骼碎片;第 2 组的骨骼受热温度为 20°C;第 3 组的骨骼受热温度为 100°C;第 4 组的骨骼受热温度为 200°C。采用改良弗罗斯特手工骨制备法制备磨碎的骨切片。使用 ImageJ 软件对显微照片进行形态计量分析。 数据使用 SPSS 软件进行分析。与未受热的样本相比,受热的骨样本的哈弗氏管面积和直径的平均值有不明显的增加。骨细胞数量略高,但差异无统计学意义(P > 0.05)。 结果表明,在 200°C 的温度下,骨骼的组织形态特征不会发生改变。这一信息对法医调查非常有用。
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引用次数: 0
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Advances in Biomedical and Health Sciences
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