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Insight into the Biostimulant Effect of an Aqueous Duckweed Extract on Tomato Plants 揭示鸭茅水提取物对番茄植株的生物刺激作用
Pub Date : 2024-05-23 DOI: 10.3390/agriculture14060808
Dario Priolo, C. Tolisano, Monica Brienza, D. Del Buono
Agricultural systems must improve their sustainability and productivity to meet the growing global demand for food. A cost-effective and sustainable way is the development of biostimulants from plants rich in bioactive compounds. This study aimed to test an aqueous extract from Lemna minor L. (duckweed) on tomato plants at different concentrations (LE—0.1, 0.5 and 1.0%—weight/volume, w/v). Photosystem I and II activity, linear electron flow (LEF), electrochemical gradient across the thylakoid membrane (ECSt), shoot biomass production, root phenotyping, pigment and metabolite content were studied. LE improved many of these traits, with LE 0.5% being the most effective dosage. Compared to the untreated samples, LE significantly stimulated photosystems to use light energy while reducing the amount lost as heat (PhiNPQ and NPQt) or potentially toxic to chloroplasts (PhiNO). These results were supported by the improved shoot biomass production (number of leaves and fresh and dry weight) and root traits (number of tips, surface, volume and fresh and dry weight) found for LE-treated samples compared to untreated controls. Finally, the study highlighted that LE increased pigment and flavonoid contents. In conclusion, the research indicates that this species can be an effective and eco-friendly tool to stimulate beneficial responses in tomato.
农业系统必须提高其可持续性和生产力,以满足全球日益增长的粮食需求。从富含生物活性化合物的植物中开发生物刺激剂是一种具有成本效益且可持续的方法。本研究旨在测试不同浓度(LE-0.1、0.5 和 1.0%-重量/体积,w/v)的小浮萍水提取物对番茄植株的影响。研究内容包括光系统 I 和 II 活性、线性电子流(LEF)、跨类木质膜电化学梯度(ECSt)、芽生物量产量、根表型、色素和代谢物含量。LE改善了许多性状,LE 0.5%是最有效的剂量。与未处理的样品相比,LE 能显著促进光合系统利用光能,同时减少热量损失(PhiNPQ 和 NPQt)或对叶绿体的潜在毒性损失(PhiNO)。与未经处理的对照组相比,经 LE 处理的样品的嫩枝生物量产量(叶片数量、鲜重和干重)和根系性状(根尖数量、表面、体积、鲜重和干重)均有所提高,这也证明了上述结果。最后,研究还强调,LE 增加了色素和类黄酮的含量。总之,研究表明,该物种可以作为一种有效且环保的工具,激发番茄的有益反应。
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引用次数: 0
A Hive Entrance System That Directs Honey Bees Inside or Outside a Greenhouse Reduced Colony Decline While Effectively Pollinating Zucchini Squash 引导蜜蜂进入温室内部或外部的蜂巢入口系统 在有效为西葫芦授粉的同时,减少了蜂群衰退现象
Pub Date : 2024-05-23 DOI: 10.3390/agriculture14060805
Cássia R. A. Gomes, Mateus A. M. Batista, Yara M. M. Ferraz, Matheus F. Trivellato, Gustavo A. Siniscalchi, G. V. Polycarpo, E. Rigobelo, David De Jong, Daniela Nicodemo
Honey bee colonies rapidly decline when confined to greenhouses, increasing pollination rental costs as they need to be replaced frequently. We tested a hive system with entrances that can be manipulated to direct bees inside or outside greenhouses containing a zucchini crop. In one greenhouse, the bees could only forage inside for 15 days; in another, bees were directed to the inside from 5 to 9 a.m., after which they only foraged outside. This procedure was repeated two more times in each greenhouse with new hives. Data were collected on how the number of bee flower visits affected fruit production, the frequency of flower visits, and the amount of bee brood and food in the hives. Flowers visited by bees four times or more set more and larger fruit. The frequency of flower visits by bees from the hives confined to the greenhouse was reduced after eight days; it was not reduced in the greenhouse with bees that could forage outside. The bee brood area was reduced in the colonies that were confined to the greenhouse, while it was maintained in the semi-confined hives. The hives with controllable entrances proved effective for pollination, while causing less damage to the bees.
蜜蜂群被限制在温室内会迅速减少,由于需要经常更换蜜蜂,授粉的租金成本也会增加。我们对一种蜂巢系统进行了测试,该系统的入口可进行调节,引导蜜蜂进入或离开种植西葫芦的温室。在一个温室中,蜜蜂只能在室内觅食 15 天;在另一个温室中,蜜蜂从早上 5 点到 9 点被引导到室内,之后只能在室外觅食。这个过程在每个温室中用新蜂箱重复两次。收集的数据包括蜜蜂访花次数对果实产量的影响、访花频率以及蜂巢中的育雏和食物数量。蜜蜂访花四次或四次以上的花结出的果实更多、更大。被限制在温室中的蜂箱的蜜蜂访花次数在八天后减少;而在可以在室外觅食的温室中,蜜蜂的访花次数并没有减少。被限制在温室中的蜂群的育雏面积减少了,而半封闭蜂箱的育雏面积保持不变。事实证明,入口可控的蜂箱能有效授粉,同时对蜜蜂造成的损害较小。
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引用次数: 0
Evidence of Allelopathy among Selected Moss Species with Lettuce and Radish 选定苔藓物种与莴苣和萝卜之间的同位异化作用证据
Pub Date : 2024-05-23 DOI: 10.3390/agriculture14060812
Nikolina A. Matić, Marija V. Ćosić, Djordje P. Božović, Silvia Poponessi, Sara D. Pavkov, Michal Goga, Milorad M. Vujičić, A. Sabovljević, Marko S. Sabovljević
There is limited evidence on bryophyte-tracheophyte allelopathic interactions. Even less is known about such relationships among commercially important plants and mosses. With the aim of screening such interactions, various extract concentrations of nine different mosses were tested on the seed germination and seedlings, i.e., hypocotyl elongation and total chlorophyll content of lettuce and radish. The allelopathic effects are documented to be pairwise (moss-vegetable) and species-specific. Based on the results, the extracts of Leucodon sciuroides and Dicranum polysetum are not harmful to lettuce and radish. Lower extract concentrations of Leucodon sciuroides and Ctenidium molluscum have a positive effect on lettuce development, while those of Thuidium delicatulum, Ctenidium molluscum, and Dicranum polysetum showed to be effective on radish. Further, negative effects were noticed when applying higher extract concentration of Abietinella abietina, Isothecium alopecuroides, Dicranum polysetum, and Racomitrium elongatum to lettuce and Isothecium alopecuroides to radish. The dataset presented in this study offers numerous possibilities for further target pest/vegetable type applications since some of the moss extracts are shown to be positive, negative, or indifferent to the tested features in lettuce and radish.
关于红叶植物-蛛形纲植物等位病理学相互作用的证据很有限。对于具有重要商业价值的植物与苔藓之间的这种关系,了解得就更少了。为了筛选这种相互作用,我们测试了九种不同苔藓的各种提取物浓度对莴苣和萝卜的种子萌发和幼苗(即下胚轴伸长和总叶绿素含量)的影响。结果表明,等位效应是成对的(苔藓-蔬菜),并具有物种特异性。根据研究结果,Leucodon sciuroides 和 Dicranum polysetum 的提取物对莴苣和萝卜无害。较低浓度的 Leucodon sciuroides 和 Ctenidium molluscum 提取物对莴苣的生长发育有积极影响,而 Thuidium delicatulum、Ctenidium molluscum 和 Dicranum polysetum 提取物则对萝卜有效。此外,当对莴苣施用较高浓度的 Abietinella abietina、Isothecium alopecuroides、Dicranum polysetum 和 Racomitrium elongatum 提取物时,以及对萝卜施用较高浓度的 Isothecium alopecuroides 提取物时,都会产生负面影响。本研究提供的数据集为进一步的目标害虫/蔬菜类型应用提供了多种可能性,因为有些苔藓提取物对莴苣和萝卜的测试特征显示出阳性、阴性或无关性。
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引用次数: 0
Perceived Benefit, Policy Incentive and Farmers’ Organic Fertilizer Application in Protected Areas 保护区内农民的利益感知、政策激励和有机肥施用情况
Pub Date : 2024-05-23 DOI: 10.3390/agriculture14060810
Jie Yang, K. Su, Ziyi Zhang, Sihan Guo, Yilei Hou, Yali Wen
The green production behaviors of farmers in protected areas (PAs) can reduce environmental disturbances and contribute to the effectiveness of PAs. Based on a survey of 708 farmers in Wuyishan National Park (WNP) and Crested Ibis Nature Reserve (CINR), we studied the influence of perceived benefit and policy incentive on farmers’ organic fertilizer application. Results: (1) Perceived benefit, subsidies policy, and propaganda policy significantly promoted farmers’ organic fertilizer application, with perceived benefit having the strongest effect, followed by subsidies policy. The influence of restriction policy was not significant. (2) Perceived benefit, subsidies policy, and propaganda policy indirectly influenced organic fertilizer application through ecological awareness. (3) In WNP, perceived benefit had the strongest effect on organic fertilizer application, and subsidies policy significantly enhanced farmers’ ecological awareness and influenced their behaviors. In contrast, subsidies policy had the strongest effect on organic fertilizer application, but did not significantly enhance farmers’ ecological awareness in CINR. These results offer valuable insights for promoting green production behaviors of farmers in PAs. This study implies that there must be a pathway for the realization of ecological value in ecological protection. Ecological value realization is the endogenous motivation for farmers’ sustainable green production behaviors. However, government subsidies and guidance are also essential in the early stage.
保护区内农民的绿色生产行为可以减少对环境的干扰,有助于提高保护区的效益。基于对武夷山国家公园(WNP)和朱鹮自然保护区(CINR)708 位农民的调查,我们研究了感知利益和政策激励对农民施用有机肥的影响。结果:(1)感知利益、补贴政策和宣传政策对农民施用有机肥有显著促进作用,其中感知利益的作用最强,其次是补贴政策。限制政策的影响不明显。(2) 感知效益、补贴政策和宣传政策通过生态意识间接影响有机肥施用量。(3) 在 WNP 中,感知效益对有机肥施用量的影响最大,补贴政策显著增强了农民的生态意识并影响了他们的行为。相比之下,在中国农村地区,补贴政策对有机肥施用量的影响最大,但对农民生态意识的提高并不明显。这些结果为促进保护区农民的绿色生产行为提供了有价值的启示。本研究表明,生态保护中必须有生态价值的实现途径。生态价值实现是农民可持续绿色生产行为的内生动力。然而,在初期阶段,政府的补贴和引导也是必不可少的。
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引用次数: 0
Economic Implications of Government Flood Control Policy: A Case of Rice in Japan 政府防洪政策的经济影响:日本水稻案例
Pub Date : 2024-05-23 DOI: 10.3390/agriculture14060814
Shinichi Kurihara, Yuki Yano, Atsushi Maruyama
Japan’s susceptibility to and severity of floods have necessitated flood control policies by the government. “Overflowing flood control”, in which the floods due to torrential rains are systematically diverted to agricultural lands in the upper to middle reaches, is one of them. More information is needed on the public assessment of the overflowing flood control policy, and this research seeks to bridge this gap. Data evaluating rice affected by the policy were collected from a random nth-price auction using a developed online system. The sample consisted of 47 consumers living in the downstream areas of the Edogawa River, one of Japan’s first-class, or prime, rivers. Data on their attitudes toward the policy were collected with a questionnaire. Multiple ordered probit models are used for regression analysis. The results show that the sample respondents were willing to pay an average of JPY 1578 for 5 kg of rice, slightly higher than the national average rice production cost, and that 36% of the sample agreed with the flood control policy, which is positively associated with large families or owning many assets.
日本易受洪水侵袭,且洪水严重,因此政府必须采取防洪政策。其中之一就是 "溢流防洪",即有计划地将暴雨引起的洪水引向中上游的农田。公众对溢洪控制政策的评价需要更多信息,本研究试图弥补这一空白。本研究利用开发的在线系统,从随机第 n 次价格拍卖中收集了受政策影响的大米评估数据。样本由居住在江户川下游地区的 47 名消费者组成,江户川是日本的一级河流(或称主要河流)之一。通过问卷调查收集了消费者对该政策的态度数据。采用多重有序 probit 模型进行回归分析。结果显示,样本受访者愿意为 5 公斤大米平均支付 1578 日元,略高于全国平均大米生产成本,36% 的样本受访者同意防洪政策,而防洪政策与大家庭或拥有许多资产呈正相关。
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引用次数: 0
Recyclable Rubber Flooring in Farrowing Crates and Its Influence on the Development and Welfare of Piglets in the Maternity Phase 产仔箱中的可回收橡胶地板及其对产期仔猪发育和福利的影响
Pub Date : 2024-05-23 DOI: 10.3390/agriculture14060807
Beatriz de Oliveira Possagnolo, Isabella Cardoso Ferreira da Silva Condotta, M. N. Amorim, Érik dos Santos Harada, Sônia Maria de Stefano Piedade, Daniele Aparecida Mendonça Cipriano, Késia Oliveira da Silva-Miranda
To boost productivity in pig production, it is essential to improve the litter size per sow. However, achieving this goal requires reducing newborn mortality rates and increasing their weight gain. In this regard, prioritizing animal welfare and adopting practices that promote sustainability in the activity is crucial. The study aimed to evaluate the sustainable flooring coverage of farrowing crates, considering piglet adaptation, thermal comfort, animal development, and increased productivity. Three types of flooring treatments were defined: T1—wood shavings; T2—solid rubber mat; T3—perforated rubber mat, for 12 litters (±50 animals/treatment). Behavior, physiology, piglet production indices, and facility microclimate were assessed. The facility’s microclimate indicated the possibility of cold stress. There was a greater preference for the mat treatments among the animals, as they remained on them for longer periods and showed increased activity in the farrowing crates. The presence of mats resulted in lower mortality due to crushing, positively contributing to production. These findings suggest the potential of using recyclable rubber mats in pig production and encourage the exploration of new designs and installation locations.
要提高养猪生产率,就必须提高每头母猪的产仔数。然而,要实现这一目标,就必须降低初生仔猪的死亡率并提高其增重。在这方面,优先考虑动物福利并采用促进活动可持续性的做法至关重要。这项研究旨在评估产仔箱地板的可持续覆盖率,同时考虑到仔猪的适应性、热舒适度、动物发育和生产率的提高。确定了三种地板处理方式:T1-木屑;T2-实心橡胶垫;T3-穿孔橡胶垫,共12窝(±50头/处理)。对行为、生理、仔猪生产指数和设施微气候进行了评估。设施的微气候表明可能存在冷应激。仔猪更喜欢垫料处理,因为它们在垫料上停留的时间更长,在产仔箱中的活动也更多。垫子的存在降低了因挤压造成的死亡率,对提高产量起到了积极作用。这些研究结果表明,在养猪生产中使用可回收橡胶垫具有潜力,并鼓励探索新的设计和安装位置。
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引用次数: 0
Sustainable Livestock Production: Screening Analysis and Pilot Implementation of a Biofilm in Piggery Biofilters for Mitigation of Ammonia and Hydrogen Sulfide Emissions 可持续畜牧生产:养猪场生物过滤器中生物膜的筛选分析和试点实施,以减少氨和硫化氢排放
Pub Date : 2024-05-23 DOI: 10.3390/agriculture14060806
B. Breza-Boruta, Piotr Kanarek, Zbigniew Paluszak, Anna-Karina Kaczorowska, G. Gryń
One of the most noticeable problems associated with the close location of piggeries is gaseous compounds emission. Ammonia and hydrogen sulfide emissions affect the quality of life of people living in the vicinity of such facilities. Among the diverse methods for managing and controlling malodorous substances, biological methods, which involve the utilization of microbiological agents, are widely employed. The use of bacterial strains is a relatively simple, low-cost, and ecological method. The study aimed to conduct a preliminary evaluation of the implementation of a novel consortium of deodorizing bacteria. The study involved the selection of bacteria, assessment of the antagonistic properties, implementation of the inoculum in a mesh-filled biofilter, and analysis of ammonia, hydrogen sulfide, and fine dust content in the air before and after passing through the mature biological bed. The results obtained demonstrate the effectiveness of the biofiltration bed in reducing ammonia levels, with a maximum decrease observed at 73.90%. For hydrogen sulfide, a removal efficiency of >72.08% was observed. Reduction in fine dust pollution also decreased from a level of 3.75 mg/m3 to 1.06 mg/m3. The study’s findings demonstrate the promising potential of utilizing a consortium of deodorizing bacteria as an effective approach to mitigating emissions from piggeries.
养猪场附近最明显的问题之一是气体化合物的排放。氨气和硫化氢的排放会影响这些设施附近居民的生活质量。在管理和控制恶臭物质的各种方法中,涉及利用微生物制剂的生物方法被广泛采用。使用细菌菌株是一种相对简单、低成本和生态的方法。这项研究旨在对新型除臭细菌群的实施情况进行初步评估。研究包括选择细菌、评估拮抗特性、在网状生物过滤器中实施接种物,以及分析通过成熟生物床前后空气中的氨、硫化氢和微尘含量。结果表明,生物过滤床在降低氨含量方面非常有效,最大降幅达到 73.90%。硫化氢的去除率大于 72.08%。微尘污染也从 3.75 毫克/立方米降至 1.06 毫克/立方米。研究结果表明,利用除臭细菌群作为一种有效方法来减少养猪场的排放,具有广阔的前景。
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引用次数: 0
Amino Acid Changes during Maturation in Solanum Fruit 茄果实成熟过程中的氨基酸变化
Pub Date : 2024-05-22 DOI: 10.3390/agriculture14060802
Jūratė Staveckienė, Brigita Medveckienė, Viktorija Vaštakaitė-Kairienė, Jurgita Kulaitienė, Elvyra Jarienė
The goal of this study is to assess the impacts of ripening stage of four Solanum fruit species: (Solanum melanocerasum, Solanum nigrum, Solanum villosum, and Solanum retroflexum) on the content of amino acids and protein. Our objective is to enhance comprehension of the maturity process, with a particular focus on Solanum fruits, in order to determine the most advantageous time for harvesting. Amino acids play a crucial role in human nutrition by serving as building blocks for various primary and secondary metabolites. They are either a source of nutraceutical substances or important dietary components. The studied Solanum fruit’s amino acid profiles were found essential and nonessential amino acids. Our findings showed that dominant amino acids were nonessential amino acids. Depending on the ripening stage, the nonessential amino acid content of the Solanum melanocerasum fruits varied from 8.22 to 9.25 g 100 g−1, Solanum villosum from 5.34 to 6.60 g 100 g−1, Solanum nigrum from 6.12 to 8.73 g 100 g−1, and Solanum retroflexum from 8.27 to 9.75 g 100 g−1. A differentiated level of total protein is found in Solanum fruits at different ripening stages. The interval was from 10.62 to 28.06 g 100 g−1 depending on species or ripening stages.
本研究的目的是评估四种茄果类(黑茄、黑茄、茄果和茄果)的成熟阶段对氨基酸和蛋白质含量的影响。我们的目标是加深对成熟过程的理解,特别是对茄果类的理解,以便确定最有利的采收时间。氨基酸作为各种初级和次级代谢产物的组成成分,在人类营养中发挥着至关重要的作用。它们既是营养保健物质的来源,也是重要的膳食成分。所研究的茄果类水果的氨基酸谱中发现了必需氨基酸和非必需氨基酸。我们的研究结果表明,主要氨基酸为非必需氨基酸。根据成熟阶段的不同,黑茄科植物果实的非必需氨基酸含量从 8.22 到 9.25 克 100 克-1 不等;小茄科植物从 5.34 到 6.60 克 100 克-1 不等;黑茄科植物从 6.12 到 8.73 克 100 克-1 不等;茄科植物从 8.27 到 9.75 克 100 克-1 不等。在不同的成熟阶段,茄果实中的总蛋白水平也不同。根据品种或成熟阶段的不同,总蛋白含量介于 10.62 至 28.06 g 100 g-1 之间。
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引用次数: 0
Deep Learning for Multi-Source Data-Driven Crop Yield Prediction in Northeast China 深度学习用于多源数据驱动的中国东北地区作物产量预测
Pub Date : 2024-05-22 DOI: 10.3390/agriculture14060794
Jian Lu, Jian Li, Hongkun Fu, Xuhui Tang, Zhao Liu, Hui Chen, Yue Sun, Xiangyu Ning
The accurate prediction of crop yields is crucial for enhancing agricultural efficiency and ensuring food security. This study assesses the performance of the CNN-LSTM-Attention model in predicting the yields of maize, rice, and soybeans in Northeast China and compares its effectiveness with traditional models such as RF, XGBoost, and CNN. Utilizing multi-source data from 2014 to 2020, which include vegetation indices, environmental variables, and photosynthetically active parameters, our research examines the model’s capacity to capture essential spatial and temporal variations. The CNN-LSTM-Attention model integrates Convolutional Neural Networks, Long Short-Term Memory, and an attention mechanism to effectively process complex datasets and manage non-linear relationships within agricultural data. Notably, the study explores the potential of using kNDVI for predicting yields of multiple crops, highlighting its effectiveness. Our findings demonstrate that advanced deep-learning models significantly enhance yield prediction accuracy over traditional methods. We advocate for the incorporation of sophisticated deep-learning technologies in agricultural practices, which can substantially improve yield prediction accuracy and food production strategies.
准确预测作物产量对于提高农业效率和确保粮食安全至关重要。本研究评估了 CNN-LSTM-Attention 模型在预测中国东北地区玉米、水稻和大豆产量方面的性能,并将其与 RF、XGBoost 和 CNN 等传统模型的效果进行了比较。利用从 2014 年到 2020 年的多源数据(包括植被指数、环境变量和光合作用参数),我们的研究考察了该模型捕捉基本时空变化的能力。CNN-LSTM-Attention 模型集成了卷积神经网络、长短期记忆和注意力机制,可有效处理复杂的数据集,并管理农业数据中的非线性关系。值得注意的是,该研究探索了利用 kNDVI 预测多种作物产量的潜力,突出了其有效性。我们的研究结果表明,与传统方法相比,先进的深度学习模型大大提高了产量预测的准确性。我们提倡在农业实践中采用先进的深度学习技术,这样可以大大提高产量预测的准确性,并改善粮食生产战略。
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引用次数: 0
Research on a Multi-Lens Multispectral Camera for Identifying Haploid Maize Seeds 用于识别单倍体玉米种子的多镜头多光谱照相机研究
Pub Date : 2024-05-22 DOI: 10.3390/agriculture14060800
Xiantao He, Jinting Zhu, Pinxuan Li, Dongxing Zhang, Li Yang, Tao Cui, Kailiang Zhang, Xiaolong Lin
Haploid breeding can shorten the breeding period of new maize varieties and is an important means to increase maize yield. In the breeding program, a large number of haploid seeds need to be screened, and this step is mainly achieved manually, which hinders the industrialization of haploid maize breeding. This article aims to develop a multispectral camera to identify the haploid seeds automatically. The camera was manufactured by replacing narrow-band filters of the ordinary CCD camera, and the RGB, 405 nm, 980 nm and 1050 nm images of haploid or diploid seeds were simultaneously captured (the characteristic wavelengths were determined according to color and high-oil markers of maize). The performance was tested using four maize varieties with the two genetic markers. The results show that the developed multispectral camera significantly improved the recognition accuracy of haploid maize seeds to 92.33%, 97.33%, 97% and 93.33% for the TYD1903, TYD1904, TYD1907 and TYD1908 varieties, respectively. The cameras in the near-infrared region (wavelengths of 980 nm and 1050 nm) achieved better performance for the varieties of high-oil marker, with an increase of 0.84% and 1.5%, respectively. These results demonstrate the strong potential of the multispectral imaging technology in the haploid seed identification of maize.
单倍体育种可以缩短玉米新品种的培育周期,是提高玉米产量的重要手段。在育种计划中,需要筛选大量单倍体种子,而这一步骤主要由人工完成,这阻碍了单倍体玉米育种的产业化。本文旨在开发一种自动识别单倍体种子的多光谱相机。该相机是通过替换普通 CCD 相机的窄带滤光片制造的,可同时拍摄单倍体或二倍体种子的 RGB、405 nm、980 nm 和 1050 nm 图像(特征波长是根据玉米的颜色和高油标记确定的)。使用带有两种遗传标记的四个玉米品种对其性能进行了测试。结果表明,所开发的多光谱相机大大提高了对单倍体玉米种子的识别准确率,对 TYD1903、TYD1904、TYD1907 和 TYD1908 品种的识别准确率分别为 92.33%、97.33%、97% 和 93.33%。近红外区域(波长为 980 纳米和 1050 纳米)的照相机对高油分标记品种的表现更好,分别提高了 0.84% 和 1.5%。这些结果证明了多光谱成像技术在玉米单倍体种子鉴定中的强大潜力。
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引用次数: 0
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