Abstracts: Objective: To examine the significance of plasma Transforming Growth Factor-1/TGF-1 (TGF-1) level testing in patients with Type 2 Diabetes Mellitus (T2DM) and heart failure. Methods: A sample of T2DM patients who were hospitalised for dyspnea between June 2021 and June 2023 were chosen. Based on the convenience sample approach, 150 cases were screened for the study, and 50 healthy non-diabetic people without cardiac problems who completed physical examinations over the same period were included as a control group. All study participants had their serum NT-proBNP and plasma TGF-I levels checked, and the values between the two groups were compared. Then, the patients with T2DM with heart failure were grouped according to whether they were accompanied by heart failure or not and the grading of cardiac function, and then the serum NT-proBNP and plasma TGF-β1 levels were compared between the different groups of patients. The diagnostic value of plasma TGF-β1 in the occurrence of heart failure in patients with T2DM was analysed. Results: There were 54 patients without heart failure and 96 people with heart failure among the 150 T2DM patients. The cut-off point was 44.50 g/L. At this time, the sensitivity and specificity for the diagnosis of concomitant heart failure in T2DM were 79.63% and 52.51%, respectively. 96 individuals with T2DM and heart failure showed greater serum and plasma levels of NT-proBNP and TGF-1 compared to the other two groups (P 0.05). ProBNP levels and plasma TGF-1 levels had a positive and significant relationship (P 0.05). Conclusion: Plasma TGF-1 levels were much higher in T2DM patients than in the general population, and the increase in this index was more pronounced in patients who also had heart failure, which is a diagnostic indicator for T2DM and heart failure.
{"title":". Significance of Plasma TGF-β1 Level Detection in Patients with T2DM with Heart Failure","authors":"Yunjing Sun","doi":"10.5937/jomb0-47321","DOIUrl":"https://doi.org/10.5937/jomb0-47321","url":null,"abstract":"Abstracts: Objective: To examine the significance of plasma Transforming Growth Factor-1/TGF-1 (TGF-1) level testing in patients with Type 2 Diabetes Mellitus (T2DM) and heart failure. Methods: A sample of T2DM patients who were hospitalised for dyspnea between June 2021 and June 2023 were chosen. Based on the convenience sample approach, 150 cases were screened for the study, and 50 healthy non-diabetic people without cardiac problems who completed physical examinations over the same period were included as a control group. All study participants had their serum NT-proBNP and plasma TGF-I levels checked, and the values between the two groups were compared. Then, the patients with T2DM with heart failure were grouped according to whether they were accompanied by heart failure or not and the grading of cardiac function, and then the serum NT-proBNP and plasma TGF-β1 levels were compared between the different groups of patients. The diagnostic value of plasma TGF-β1 in the occurrence of heart failure in patients with T2DM was analysed. Results: There were 54 patients without heart failure and 96 people with heart failure among the 150 T2DM patients. The cut-off point was 44.50 g/L. At this time, the sensitivity and specificity for the diagnosis of concomitant heart failure in T2DM were 79.63% and 52.51%, respectively. 96 individuals with T2DM and heart failure showed greater serum and plasma levels of NT-proBNP and TGF-1 compared to the other two groups (P 0.05). ProBNP levels and plasma TGF-1 levels had a positive and significant relationship (P 0.05). Conclusion: Plasma TGF-1 levels were much higher in T2DM patients than in the general population, and the increase in this index was more pronounced in patients who also had heart failure, which is a diagnostic indicator for T2DM and heart failure.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"40 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141348755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To study the effectiveness of individualized comprehensive nutritional support on inflammatory markers, serum amylase (AMS), prealbumin (PA), albumin (ALB), calcium ion (Ca2+) in patients with severe acute pancreatitis (SAP). Methods: 102 participants with SAP treated in our hospital were chosen as the study objects. The participants were randomly split into control group and observation group, and both groups were given routine treatment. Observation group received individualized comprehensive nutrition support additionally. The inflammatory indexes, biochemical indexes and symptom improvement time were observed and analyzed on the day of admission, intervention 1d, intervention 3d, intervention 7d and intervention 14d. Results: Among the 102 patients included in this study, 3 cases had sudden exacerbation during the intervention, 1 case had clinical data missing >10%, and 1 case voluntarily withdrew due to personal factors, all of which were eliminated. Finally, the effective data of 97 patients were recovered. There were significant differences in the hypersensitive C-reactive protein (hs-CRP), white blood cell count (WBC), procalcitonin (PCT) and interleukin1β (IL-1β) between the two groups. The inter-group, time and interaction differences of AMS, PA, ALB and Ca2+ levels were significant different. The recovery time of abdominal pain, bowel sound, blood amylase level and urine amylase level in observation group was inferior to control group, and the differences were statistically significant (P<0.05). Conclusion: Individualized comprehensive nutritional support is more conducive to the improvement of inflammatory indexes in SAP patients, and can accelerate the symptom relief and promote the recovery of nutritional status.
{"title":"- Effects of Individualized Comprehensive Nutritional Support on Inflammatory Markers, serum amylase (AMS), prealbumin (PA), albumin (ALB), calcium ion (Ca2+) in Patients with Severe Pancreatitis","authors":"Wei Meng","doi":"10.5937/jomb0-48944","DOIUrl":"https://doi.org/10.5937/jomb0-48944","url":null,"abstract":"Objective: To study the effectiveness of individualized comprehensive nutritional support on inflammatory markers, serum amylase (AMS), prealbumin (PA), albumin (ALB), calcium ion (Ca2+) in patients with severe acute pancreatitis (SAP). Methods: 102 participants with SAP treated in our hospital were chosen as the study objects. The participants were randomly split into control group and observation group, and both groups were given routine treatment. Observation group received individualized comprehensive nutrition support additionally. The inflammatory indexes, biochemical indexes and symptom improvement time were observed and analyzed on the day of admission, intervention 1d, intervention 3d, intervention 7d and intervention 14d. Results: Among the 102 patients included in this study, 3 cases had sudden exacerbation during the intervention, 1 case had clinical data missing >10%, and 1 case voluntarily withdrew due to personal factors, all of which were eliminated. Finally, the effective data of 97 patients were recovered. There were significant differences in the hypersensitive C-reactive protein (hs-CRP), white blood cell count (WBC), procalcitonin (PCT) and interleukin1β (IL-1β) between the two groups. The inter-group, time and interaction differences of AMS, PA, ALB and Ca2+ levels were significant different. The recovery time of abdominal pain, bowel sound, blood amylase level and urine amylase level in observation group was inferior to control group, and the differences were statistically significant (P<0.05). Conclusion: Individualized comprehensive nutritional support is more conducive to the improvement of inflammatory indexes in SAP patients, and can accelerate the symptom relief and promote the recovery of nutritional status.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":" 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141366362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Cheng, Lichao Li, Yafei Lv, Long Zhang, Wenhua Chen, Gongda Xu
Background: Investigate the correlation between low-density lipoprotein (LDL) cholesterol, homocysteine and cognitive function in patients with cerebral small vessel disease (CSVD). Methods: 240 patients with CSVD confirmed by head MRI in the Department of Neurology from January 2020 to December 2023 were retrospectively included in the study. All the patients had complete blood biochemical examination, and their cognitive function was evaluated by Montreal Cognitive Assessment Scale (MoCA), and after correcting for the factor of years of education, the patients were divided into a group of normal cognition (MoCA ≥26, 70 patients) and a group of cognitive function (MoCA ≥26, 70 patients) according to the scores. After correcting for the factor of years of education, the patients were divided into the normal cognitive function group (70 cases with MoCA ≥26) and the cognitive dysfunction group (170 cases with MoCA <26) according to their scores. The general information of the two groups and the patients' cognitive function characteristics, including visuospatial and executive ability, naming, attention and calculation, language, abstraction, delayed memory, and orientation, were compared, and the independent influences on the occurrence of cognitive dysfunction in patients with CSVD were analyzed by two-category multifactorial logistic regression. Results: Compared with the group with normal cognitive function, the cognitive dysfunction group had lower years of education and higher homocysteine, and the differences were statistically significant (P < 0.05). Compared with the group with normal cognitive functioning, the cognitive dysfunction group had lower MoCA total scores, lower visuospatial and executive ability, naming, attention and calculation, language, abstraction, delayed memory, and orientation scores, and the differences were statistically significant (P < 0.05). Two-category multifactorial logistic regression analysis showed that low-density lipoprotein cholesterol (OR=2.756, 95% CI:0.673-0.938, P=0.012) and homocysteine (OR=1.859, 95% CI: 1.024-1.324, P=0.016) were the independent factors influencing cognitive dysfunction in CSVD patients. The lower the risk of cognitive impairment in CSVD patients, the higher the plasma LDL cholesterol and homocysteine levels, the higher the risk of cognitive impairment in CSVD patients. Conclusion: Plasma LDL cholesterol and homocysteine levels are associated with and may be predictors of cognitive dysfunction in patients with CSVD.
{"title":"Association between Cerebral Small Vessel Disease and Plasma Levels of LDL Cholesterol and Homocysteine: Implications for Cognitive Function","authors":"Yan Cheng, Lichao Li, Yafei Lv, Long Zhang, Wenhua Chen, Gongda Xu","doi":"10.5937/jomb0-50100","DOIUrl":"https://doi.org/10.5937/jomb0-50100","url":null,"abstract":"Background: Investigate the correlation between low-density lipoprotein (LDL) cholesterol, homocysteine and cognitive function in patients with cerebral small vessel disease (CSVD). \u0000Methods: 240 patients with CSVD confirmed by head MRI in the Department of Neurology from January 2020 to December 2023 were retrospectively included in the study. All the patients had complete blood biochemical examination, and their cognitive function was evaluated by Montreal Cognitive Assessment Scale (MoCA), and after correcting for the factor of years of education, the patients were divided into a group of normal cognition (MoCA ≥26, 70 patients) and a group of cognitive function (MoCA ≥26, 70 patients) according to the scores. After correcting for the factor of years of education, the patients were divided into the normal cognitive function group (70 cases with MoCA ≥26) and the cognitive dysfunction group (170 cases with MoCA <26) according to their scores. The general information of the two groups and the patients' cognitive function characteristics, including visuospatial and executive ability, naming, attention and calculation, language, abstraction, delayed memory, and orientation, were compared, and the independent influences on the occurrence of cognitive dysfunction in patients with CSVD were analyzed by two-category multifactorial logistic regression. \u0000Results: Compared with the group with normal cognitive function, the cognitive dysfunction group had lower years of education and higher homocysteine, and the differences were statistically significant (P < 0.05). Compared with the group with normal cognitive functioning, the cognitive dysfunction group had lower MoCA total scores, lower visuospatial and executive ability, naming, attention and calculation, language, abstraction, delayed memory, and orientation scores, and the differences were statistically significant (P < 0.05). Two-category multifactorial logistic regression analysis showed that low-density lipoprotein cholesterol (OR=2.756, 95% CI:0.673-0.938, P=0.012) and homocysteine (OR=1.859, 95% CI: 1.024-1.324, P=0.016) were the independent factors influencing cognitive dysfunction in CSVD patients. The lower the risk of cognitive impairment in CSVD patients, the higher the plasma LDL cholesterol and homocysteine levels, the higher the risk of cognitive impairment in CSVD patients. \u0000Conclusion: Plasma LDL cholesterol and homocysteine levels are associated with and may be predictors of cognitive dysfunction in patients with CSVD.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":" 38","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141370530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ankang Hu, Xin Wang, Lisi Ai, Kun Liu, Lingxue Kong
Background: This study aimed to explore the correlation between the MMP-3 1171 5A/6A gene polymorphism and susceptibility to Chronic Periodontitis (CP). Methods: Following the PRISMA guidelines, a systematic search was conducted across four electronic databases (PubMed, Embase, Web of Science, and Cochrane Library) without any time or language limitations. The selection criteria included case-control studies examining the association between the MMP-3 gene polymorphism and CP. The data were independently extracted and cross-checked by two reviewers. The Newcastle-Ottawa Scale (NOS) was used to assess the quality of the studies. Statistical heterogeneity and publication bias were assessed. Results: Five studies, published between 2004 and 2019, met the inclusion criteria for the meta-analysis. No significant association was observed between MMP-3 gene polymorphism and CP susceptibility across all subjects in the four gene models. However, subgroup analysis revealed significant differences based on genotyping methods and smoking habits. Using PCR-RFLP genotyping method, the allele and additive models showed a positive correlation with the risk of CP (5A vs 6A, OR=1.12, 95%CI (1.02~1.23); 5A5A vs 6A6A, OR=2.85, 95%CI (1.61~4.86)). In contrast, using Sanger sequencing method, the 5A mutation appeared to reduce CP susceptibility (5A vs 6A, OR=0.77, 95%CI (0.67~0.87); 5A5A vs 6A6A, OR=0.20, 95%CI (0.09~0.42)). Moreover, smoking habits appeared to modulate the risk. Among smokers, the 5A mutation increased susceptibility to CP, while among non-smokers it decreased. Conclusions: While no significant correlation was found in the overall population, the stratified analysis revealed nuanced relationships contingent on genotyping methods and smoking habits.
研究背景本研究旨在探讨 MMP-3 1171 5A/6A 基因多态性与慢性牙周炎（CP）易感性之间的相关性。研究方法按照 PRISMA 指南，在四个电子数据库（PubMed、Embase、Web of Science 和 Cochrane Library）中进行了系统检索，没有任何时间或语言限制。选择标准包括研究 MMP-3 基因多态性与 CP 之间关系的病例对照研究。数据由两名审稿人独立提取和交叉核对。采用纽卡斯尔-渥太华量表（NOS）评估研究质量。评估了统计异质性和发表偏倚。研究结果2004年至2019年期间发表的五项研究符合荟萃分析的纳入标准。在四个基因模型中，所有受试者的 MMP-3 基因多态性与 CP 易感性之间均未发现明显关联。不过，亚组分析显示，不同基因分型方法和吸烟习惯的受试者之间存在显著差异。采用 PCR-RFLP 基因分型方法，等位基因和加性模型与 CP 风险呈正相关（5A vs 6A，OR=1.12，95%CI (1.02~1.23)；5A5A vs 6A6A，OR=2.85，95%CI (1.61~4.86)）。相比之下，使用 Sanger 测序方法，5A 突变似乎降低了 CP 易感性（5A vs 6A，OR=0.77，95%CI (0.67~0.87)；5A5A vs 6A6A，OR=0.20，95%CI (0.09~0.42)）。此外，吸烟习惯似乎也能调节风险。在吸烟者中，5A 突变增加了对 CP 的易感性，而在非吸烟者中则降低了易感性。结论：虽然在总体人群中没有发现明显的相关性，但分层分析显示了与基因分型方法和吸烟习惯有关的细微关系。
{"title":"Association between Matrix Metalloproteinase-3 Gene Polymorphism and Susceptibility to Chronic Periodontitis: A systematic review and meta-analysis","authors":"Ankang Hu, Xin Wang, Lisi Ai, Kun Liu, Lingxue Kong","doi":"10.5937/jomb0-49044","DOIUrl":"https://doi.org/10.5937/jomb0-49044","url":null,"abstract":"Background: This study aimed to explore the correlation between the MMP-3 1171 5A/6A gene polymorphism and susceptibility to Chronic Periodontitis (CP). \u0000Methods: Following the PRISMA guidelines, a systematic search was conducted across four electronic databases (PubMed, Embase, Web of Science, and Cochrane Library) without any time or language limitations. The selection criteria included case-control studies examining the association between the MMP-3 gene polymorphism and CP. The data were independently extracted and cross-checked by two reviewers. The Newcastle-Ottawa Scale (NOS) was used to assess the quality of the studies. Statistical heterogeneity and publication bias were assessed. \u0000Results: Five studies, published between 2004 and 2019, met the inclusion criteria for the meta-analysis. No significant association was observed between MMP-3 gene polymorphism and CP susceptibility across all subjects in the four gene models. However, subgroup analysis revealed significant differences based on genotyping methods and smoking habits. Using PCR-RFLP genotyping method, the allele and additive models showed a positive correlation with the risk of CP (5A vs 6A, OR=1.12, 95%CI (1.02~1.23); 5A5A vs 6A6A, OR=2.85, 95%CI (1.61~4.86)). In contrast, using Sanger sequencing method, the 5A mutation appeared to reduce CP susceptibility (5A vs 6A, OR=0.77, 95%CI (0.67~0.87); 5A5A vs 6A6A, OR=0.20, 95%CI (0.09~0.42)). Moreover, smoking habits appeared to modulate the risk. Among smokers, the 5A mutation increased susceptibility to CP, while among non-smokers it decreased. \u0000Conclusions: While no significant correlation was found in the overall population, the stratified analysis revealed nuanced relationships contingent on genotyping methods and smoking habits.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141368653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Lippi, L. Pighi, G. Salvagno, Roberta Ferraro, Giovanni Celegon, B. Henry
Background. Minimizing air aspiration by carefully filling blood gas syringes is crucial to prevent air contamination from causing undesirable variations in gasses and other molecules. While some previous studies investigated this aspect, these are now outdated and only analyzed a limited number of blood gas parameters. Thus, we investigated the effects air contamination in the syringe using a modern blood gas analyzer. Methods. We sampled venous blood from 17 laboratory workers (mean age: 46±11 years; 10 women), filling two consecutive blood gas syringes. The first was filled exactly to its nominal volume (i.e., 1.0 mL), while the second was filled with 0.8 mL of blood and 0.2 mL of ambient air. Blood gas analysis was performed in each syringe using an identical analyzer. Results. In the syringe with the air bubble, we found statistically significant increase in pH (0.1%), pO2 (10.8%), SO2 (11.2%), total hemoglobin (3.0%), and hematocrit (2.7%), while values of pCO2 (-4.8%), sodium (-0.5%), and ionized calcium (-1.3%) were significantly reduced. With exception of pH, all these changes exceeded the performance specifications. Potassium, chloride, glucose, lactate, COHb and MetHb values remained unchanged. Conclusion. These findings confirm that air bubbles must be removed as soon as possible after sampling from blood gas syringes to prevent artifactual test results and misleading clinical judgment and inappropriate treatment. When blood gas syringes are received in the laboratory with air bubbles inside, the most vulnerable parameters (i.e., pO2, SO2, pCO2, sodium, ionized calcium, hematocrit and hemoglobin) should be suppressed.
{"title":"Impact of an air bubble within the syringe on test results obtained with a modern blood gas analyzer","authors":"G. Lippi, L. Pighi, G. Salvagno, Roberta Ferraro, Giovanni Celegon, B. Henry","doi":"10.5937/jomb0-49870","DOIUrl":"https://doi.org/10.5937/jomb0-49870","url":null,"abstract":"\u0000Background. Minimizing air aspiration by carefully filling blood gas syringes is crucial to prevent air contamination from causing undesirable variations in gasses and other molecules. While some previous studies investigated this aspect, these are now outdated and only analyzed a limited number of blood gas parameters. Thus, we investigated the effects air contamination in the syringe using a modern blood gas analyzer. \u0000Methods. We sampled venous blood from 17 laboratory workers (mean age: 46±11 years; 10 women), filling two consecutive blood gas syringes. The first was filled exactly to its nominal volume (i.e., 1.0 mL), while the second was filled with 0.8 mL of blood and 0.2 mL of ambient air. Blood gas analysis was performed in each syringe using an identical analyzer. \u0000Results. In the syringe with the air bubble, we found statistically significant increase in pH (0.1%), pO2 (10.8%), SO2 (11.2%), total hemoglobin (3.0%), and hematocrit (2.7%), while values of pCO2 (-4.8%), sodium (-0.5%), and ionized calcium (-1.3%) were significantly reduced. With exception of pH, all these changes exceeded the performance specifications. Potassium, chloride, glucose, lactate, COHb and MetHb values remained unchanged. \u0000Conclusion. These findings confirm that air bubbles must be removed as soon as possible after sampling from blood gas syringes to prevent artifactual test results and misleading clinical judgment and inappropriate treatment. When blood gas syringes are received in the laboratory with air bubbles inside, the most vulnerable parameters (i.e., pO2, SO2, pCO2, sodium, ionized calcium, hematocrit and hemoglobin) should be suppressed.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141118730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Backgrounds：It aims to investigate the expression level of NLRP3 inflammasome and its related cell molecules in early diabetes kidney disease (EDKD) in the elderly and its clinical application value. Methods: From October 2021 to April 2023, 50 elderly patients with T2DM (T2DM group), 50 elderly patients with EDKD (EDKD group) and 50 elderly people who passed the health check-up (healthy group) are chosenas the study subjects. Plasma NLRP3 inflammasome and related cells (blood leukocyte count, monocyte count, lymphocyte count) molecular (NT-proBNP and others) levels are tested, and Pearson correlation analysis is utilized to explore the correlation among plasma NLRP3 inflammasome and related cells, molecules and renal function indicators (UACR, BUN, Ucr) in elderly patients with EDKD. Results: (1) The three groups’ comparison in HbA1c, FIns, HOMA-IR, UACR, BUN, Ucr, SOD, MCP-1 and TNF-α level were with P<0.05.The levels of TG and LDL-C in the EDKD group were bigger than those in the T2DM and the healthy groups;The levels of FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-α in the EDKD and T2DM groups werebigger than those in the healthy group, while SOD wassmaller than that in the healthy group; The levels of BUN, Ucr, hs-CRP, FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-α in the EDKD group werebigger than those in the T2DM group, while SOD was smallerthan that in the T2DM group;The aboveresults were with P<0.05. (2)It has P<0.05 in Monocyte count, NLRP3, NT-proBNP, caspase-1, ASC and othersin the three groups.The levels of thosein the EDKD and T2DM groups were bigger than the healthy group. The levels of these indicators in the EDKD group were bigger than those in the T2DM group, and with P<0.05.These indicators in EDKD group were positively correlated with UACR, BUN, and Ucr, respectively, and with P<0.05. Conclusion: NLRP3 inflammasome and its related molecules caspase-1, ASC, IL-1β, IL-18 level and Monocyte count raised in early senile EDKD, and were significantly correlated with the severity of EDKD.
{"title":". Clinical Significance of NLRP3 Inflammasome and Related Cell Moleculesin early Elderly Diabetic Kidney Disease","authors":"Yan Shang","doi":"10.5937/jomb0-45950","DOIUrl":"https://doi.org/10.5937/jomb0-45950","url":null,"abstract":"Backgrounds：It aims to investigate the expression level of NLRP3 inflammasome and its related cell molecules in early diabetes kidney disease (EDKD) in the elderly and its clinical application value. \u0000Methods: From October 2021 to April 2023, 50 elderly patients with T2DM (T2DM group), 50 elderly patients with EDKD (EDKD group) and 50 elderly people who passed the health check-up (healthy group) are chosenas the study subjects. Plasma NLRP3 inflammasome and related cells (blood leukocyte count, monocyte count, lymphocyte count) molecular (NT-proBNP and others) levels are tested, and Pearson correlation analysis is utilized to explore the correlation among plasma NLRP3 inflammasome and related cells, molecules and renal function indicators (UACR, BUN, Ucr) in elderly patients with EDKD. \u0000Results: (1) The three groups’ comparison in HbA1c, FIns, HOMA-IR, UACR, BUN, Ucr, SOD, MCP-1 and TNF-α level were with P<0.05.The levels of TG and LDL-C in the EDKD group were bigger than those in the T2DM and the healthy groups;The levels of FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-α in the EDKD and T2DM groups werebigger than those in the healthy group, while SOD wassmaller than that in the healthy group; The levels of BUN, Ucr, hs-CRP, FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-α in the EDKD group werebigger than those in the T2DM group, while SOD was smallerthan that in the T2DM group;The aboveresults were with P<0.05. (2)It has P<0.05 in Monocyte count, NLRP3, NT-proBNP, caspase-1, ASC and othersin the three groups.The levels of thosein the EDKD and T2DM groups were bigger than the healthy group. The levels of these indicators in the EDKD group were bigger than those in the T2DM group, and with P<0.05.These indicators in EDKD group were positively correlated with UACR, BUN, and Ucr, respectively, and with P<0.05. \u0000Conclusion: NLRP3 inflammasome and its related molecules caspase-1, ASC, IL-1β, IL-18 level and Monocyte count raised in early senile EDKD, and were significantly correlated with the severity of EDKD.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"105 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141124845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To observe the mechanism of lactate dehydrogenase (LDH) and interleukin 8 (IL-8) in pancreatic cancer pain and their correlation with pain degree. Methods: 126 patients with pancreatic cancer who visited our hospital from January 2021 to February 2023 were selected. The patients were divided into 58 patients with low pain (1~3 points) and 68 patients with high pain (4~10 points) by visual analog scale (VAS). And 50 health examinees in the same period were selected as the healthy control group. The serum LDH and IL-8 concentrations are analyzed by enzyme-linked immunosorbent assay, as well as the subjective pain grading method scoreis analyzed. The differences in LDH and IL-8 concentrations among the three groups of patients were compared. Pearson correlation analysis was used to investigate the correlation between LDH, IL-8 concentrations and patient pain. Binary logistic regression was used to determine independent risk factors for high pain, and ROC curves were used to analyze the diagnostic efficacy of each indicator. Result: The serum LDH and IL-8 concentrations in the high pain group were exceed the low pain group’s (P<0.05). The serum LDH and IL-8 concentrations in the low pain groupexceed the healthy control group’s(P<0.05). Pearson correlation analysis revealed a positive correlation between serum LDH concentration and pain grading (r=0.736, P=0.000). The serum IL-8 has positive correlation with pain grading (r=0.680, P=0.000). Serum LDH and IL-8 concentrations have positive correlation(r=0.589, P=0.000). LDH and IL-8 concentrations are independent risk factors for high pain levels (OR=1.033, 1.142, P<0.05). The logistic regression prediction model formula was used: Y=constant+B1X1+B2X2+...+BnXn to set the joint diagnostic prediction model as -12.063+0.033×LDH+0.133×IL-8. The areas under the ROC curves of LDH, IL-8, and predictive model (LDH+IL-8) in patients with high pain were 0.925, 0.945, and 0.974, respectively. The relevant standards for LDH are>190U/L, IL-8 is>36pg/mL, and the relevant standards for prediction models are>5.75. Conclusion: LDH and IL-8 participate in the pain aggravation process of pancreatic cancer, and are closely related to the pain grading. The combination of LDH and IL-8 can be used as a biological indicator to evaluate the pain severity of pancreatic cancer, and provide reference for clinical diagnosis and treatment.
{"title":"- Mechanism of LDH and IL-8 Involved in pancreatic cancer Pain and the Correlation of Pain Degree","authors":"Xiaoqing Xu","doi":"10.5937/jomb0-48160","DOIUrl":"https://doi.org/10.5937/jomb0-48160","url":null,"abstract":"Objective: To observe the mechanism of lactate dehydrogenase (LDH) and interleukin 8 (IL-8) in pancreatic cancer pain and their correlation with pain degree. Methods: 126 patients with pancreatic cancer who visited our hospital from January 2021 to February 2023 were selected. The patients were divided into 58 patients with low pain (1~3 points) and 68 patients with high pain (4~10 points) by visual analog scale (VAS). And 50 health examinees in the same period were selected as the healthy control group. The serum LDH and IL-8 concentrations are analyzed by enzyme-linked immunosorbent assay, as well as the subjective pain grading method scoreis analyzed. The differences in LDH and IL-8 concentrations among the three groups of patients were compared. Pearson correlation analysis was used to investigate the correlation between LDH, IL-8 concentrations and patient pain. Binary logistic regression was used to determine independent risk factors for high pain, and ROC curves were used to analyze the diagnostic efficacy of each indicator. Result: The serum LDH and IL-8 concentrations in the high pain group were exceed the low pain group’s (P<0.05). The serum LDH and IL-8 concentrations in the low pain groupexceed the healthy control group’s(P<0.05). Pearson correlation analysis revealed a positive correlation between serum LDH concentration and pain grading (r=0.736, P=0.000). The serum IL-8 has positive correlation with pain grading (r=0.680, P=0.000). Serum LDH and IL-8 concentrations have positive correlation(r=0.589, P=0.000). LDH and IL-8 concentrations are independent risk factors for high pain levels (OR=1.033, 1.142, P<0.05). The logistic regression prediction model formula was used: Y=constant+B1X1+B2X2+...+BnXn to set the joint diagnostic prediction model as -12.063+0.033×LDH+0.133×IL-8. The areas under the ROC curves of LDH, IL-8, and predictive model (LDH+IL-8) in patients with high pain were 0.925, 0.945, and 0.974, respectively. The relevant standards for LDH are>190U/L, IL-8 is>36pg/mL, and the relevant standards for prediction models are>5.75. Conclusion: LDH and IL-8 participate in the pain aggravation process of pancreatic cancer, and are closely related to the pain grading. The combination of LDH and IL-8 can be used as a biological indicator to evaluate the pain severity of pancreatic cancer, and provide reference for clinical diagnosis and treatment.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"2 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140654869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: It was to explore the correlation between angiotensin II (Ang II) gene and serum adiponectin expression in patients with cerebrovascular complications of H-type hypertension (HH) and its mechanism. A total of 50 cases of outpatient patients in Tianjin Fourth Central Hospital were recruited from January 2022 to June 2023 and rolled into three groups according to their blood pressure and basic information, namely HH cerebrovascular complications group, the non-H-type hypertension (NHH) group, and the healthy control (HC)group. Peripheral blood samples were taken, one sample was utilized to test for the Ang II gene and the methylation of Ang II, and the other sample was utilized to measure serum adiponectin levels, so as to analyze the relationship between serum adiponectin level and Ang II in patients with cerebrovascular complications of HH. The ratio of male to female was 8:7 in the group of cerebrovascular complications of HH, and mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) were 167.34mmHg and 112.56mmHg, respectively. In NHH group, the mean SBP was 165.89mmHg and the mean DBP was 113.47mmHg. The blood pressure of HC group was in the normal range. The Ang II content was the highest in the group with cerebrovascular complications of HH, followed by the group with NHH, and the lowest in HC group. Pyrosequencing chart of patients with cerebrovascular complications of HH showed that the content of deoxyphosphate ribose G was the highest, while the content of A was the highest in NHH patients. Moreover, the serum adiponectin level of patients with HH and NHH was superior to that of HC group, and adiponectin level between the former two groups and HC groupdiffered considerably. Ang II levels were high in patients with cerebrovascular complications of HH and were positively correlated with adiponectin levels. The incidence of cerebrovascular complications of HH may be related to Ang II level in patients.
摘要：探讨H型高血压（HH）脑血管并发症患者血管紧张素Ⅱ（AngⅡ）基因与血清脂肪连蛋白表达的相关性及其机制。研究对象为2022年1月至2023年6月在天津市第四中心医院门诊就诊的50例H型高血压脑血管并发症患者，根据血压和基本信息分为三组，即H型高血压脑血管并发症组、非H型高血压（NHH）组和健康对照（HC）组。取外周血样本，一份用于检测 Ang II 基因和 Ang II 的甲基化，另一份用于检测血清脂肪连接蛋白水平，从而分析 HH 脑血管并发症患者血清脂肪连接蛋白水平与 Ang II 的关系。HH 脑血管并发症组男女比例为 8:7，平均收缩压（SBP）和舒张压（DBP）分别为 167.34mmHg 和 112.56mmHg。NHH 组的平均收缩压（SBP）为 165.89mmHg，平均舒张压（DBP）为 113.47mmHg。HC 组的血压处于正常范围。HH 脑血管并发症组的 Ang II 含量最高，NHH 组次之，HC 组最低。HH 脑血管并发症患者的热测序图显示，脱氧磷酸核糖 G 的含量最高，而 A 的含量在 NHH 患者中最高。此外，HH 和 NHH 患者的血清脂肪连接蛋白水平均高于 HC 组，且前两组与 HC 组的脂肪连接蛋白水平差异较大。HH 脑血管并发症患者的 Ang II 水平较高，且与脂肪连接蛋白水平呈正相关。HH 脑血管并发症的发生率可能与患者体内的 Ang II 水平有关。
{"title":"the Internal mechanism of correlation between angiotensin II gene and serum adiponectin level in patients with cerebrovascular complications of H-type hypertension","authors":"Shangyu Wen","doi":"10.5937/jomb0-45532","DOIUrl":"https://doi.org/10.5937/jomb0-45532","url":null,"abstract":"Abstract: It was to explore the correlation between angiotensin II (Ang II) gene and serum adiponectin expression in patients with cerebrovascular complications of H-type hypertension (HH) and its mechanism. A total of 50 cases of outpatient patients in Tianjin Fourth Central Hospital were recruited from January 2022 to June 2023 and rolled into three groups according to their blood pressure and basic information, namely HH cerebrovascular complications group, the non-H-type hypertension (NHH) group, and the healthy control (HC)group. Peripheral blood samples were taken, one sample was utilized to test for the Ang II gene and the methylation of Ang II, and the other sample was utilized to measure serum adiponectin levels, so as to analyze the relationship between serum adiponectin level and Ang II in patients with cerebrovascular complications of HH. The ratio of male to female was 8:7 in the group of cerebrovascular complications of HH, and mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) were 167.34mmHg and 112.56mmHg, respectively. In NHH group, the mean SBP was 165.89mmHg and the mean DBP was 113.47mmHg. The blood pressure of HC group was in the normal range. The Ang II content was the highest in the group with cerebrovascular complications of HH, followed by the group with NHH, and the lowest in HC group. Pyrosequencing chart of patients with cerebrovascular complications of HH showed that the content of deoxyphosphate ribose G was the highest, while the content of A was the highest in NHH patients. Moreover, the serum adiponectin level of patients with HH and NHH was superior to that of HC group, and adiponectin level between the former two groups and HC groupdiffered considerably. Ang II levels were high in patients with cerebrovascular complications of HH and were positively correlated with adiponectin levels. The incidence of cerebrovascular complications of HH may be related to Ang II level in patients.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"10 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140225376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Multiple organ dysfunction syndrome (MODS) is common after sepsis and increases mortality. Lactate (Lac) can assess the prognosis of patients. Albumin (Alb) is closely associated with inflammatory response in sepsis patients. This work evaluated the predictive value of Lac/Alb for prognosis of sepsis patients. Materials and Methods: data of 160 sepsis patients were retrospectively collected. Lac and Alb levels were measured upon admission, at 24 hours and 48 hours later. Using 0.45 as the cutoff value for Lac/Alb, patients were rolled into high-level (HL) and low-level (LL) groups. MODS rates and mortality rates were analyzed. Receiver operating characteristic (ROC) curves were utilized to evaluate the predictive value of 48-hour Lac/Alb for patient prognosis. Correlation between Lac/Alb and APACHE II and SOFA scores was assessed. Results: the 12-month follow-up revealed 52 deaths (32.5%), and MODS occurred in 49 cases (30.6%) on the 7th day. The MODS group possessed elevated Lac and Lac/Alb and decreased Alb to the N-MODS group (P<0.05), and similar results were observed by comparison the survival and death group (P<0.05). The sensitivity, specificity, and area under the ROC curve (AUC) of Lac/Alb in predicting MODS were 81.63%, 85.59%, and 0.89, respectively, while those in predicting death were 94.23%, 88.89%, and 0.91, respectively. Lac/Alb was positively correlated with APACHE II and SOFA scores (r=0.718 and 0.808, respectively). Conclusion: Lac/Alb was linked to MODS and mortality in sepsis patients and can be based to predict adverse outcomes.
{"title":"Predictive Value of Lactate/Albumin Ratio for Death and Multiple Organ Dysfunction Syndrome in Patients with Sepsis","authors":"Shuwei Huang","doi":"10.5937/jomb0-46947","DOIUrl":"https://doi.org/10.5937/jomb0-46947","url":null,"abstract":"Introduction: Multiple organ dysfunction syndrome (MODS) is common after sepsis and increases mortality. Lactate (Lac) can assess the prognosis of patients. Albumin (Alb) is closely associated with inflammatory response in sepsis patients. This work evaluated the predictive value of Lac/Alb for prognosis of sepsis patients. Materials and Methods: data of 160 sepsis patients were retrospectively collected. Lac and Alb levels were measured upon admission, at 24 hours and 48 hours later. Using 0.45 as the cutoff value for Lac/Alb, patients were rolled into high-level (HL) and low-level (LL) groups. MODS rates and mortality rates were analyzed. Receiver operating characteristic (ROC) curves were utilized to evaluate the predictive value of 48-hour Lac/Alb for patient prognosis. Correlation between Lac/Alb and APACHE II and SOFA scores was assessed. Results: the 12-month follow-up revealed 52 deaths (32.5%), and MODS occurred in 49 cases (30.6%) on the 7th day. The MODS group possessed elevated Lac and Lac/Alb and decreased Alb to the N-MODS group (P<0.05), and similar results were observed by comparison the survival and death group (P<0.05). The sensitivity, specificity, and area under the ROC curve (AUC) of Lac/Alb in predicting MODS were 81.63%, 85.59%, and 0.89, respectively, while those in predicting death were 94.23%, 88.89%, and 0.91, respectively. Lac/Alb was positively correlated with APACHE II and SOFA scores (r=0.718 and 0.808, respectively). Conclusion: Lac/Alb was linked to MODS and mortality in sepsis patients and can be based to predict adverse outcomes.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"89 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140242204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vesile Örnek Diker, Gülseren Yılmaz, M. E. Düz, Mürvet Algemi, Mehmet Köseoğlu, Hümeyra Öztürk Emre, Osman Oğuz
Background: Vitamin D binding protein plays a crucial role in regulating vitamin D levels by carrying vitamin D and its metabolites and immunological response by binding to endotoxins and fatty acids. We aimed to compare vitamin D, DBP, and specific inflammatory markers among intensive care unit (ICU) patients with and without the COVID-19 virus. Methods: This multicenter study conducted in two training and research hospitals included 37 (13 female) COVID-19-positive and 51 (34 female) COVID-19-negative ICU patients. 25(OH) vitamin D, DBP, c-reactive protein (CRP), procalcitonin (PCT), D-dimer, troponin T (TnT), interleukin 6 (IL-6) and ferritin levels, survival, mortality rates, duration of stay (ICU) were examined. Results: We observed higher ferritin and CRP levels, along with lower DBP, TnT and D-dimer levels, in patients with COVID-19. ICU patients with COVID-19 exhibited elevated mortality rates (Odds Ratio: 3.012, 95% Confidence Interval [1.252-7.248], p=0.013). However, there was no statistically significant correlation observed between mortality rates and Vitamin D or DBP levels across the entire ICU patient cohort. Conclusions: Vitamin d values were found to be low in all intensive care patients, regardless of their covid 19 status. Contrary to the literature, COVID-19 patients had lower D-dimer and TNT levels than negative controls. However, COVID-19-positive ICU patients have decreased DBP. Further DBP gene polymorphisms studies are needed to explain this situation
{"title":"Vitamin D and Vitamin D Binding Protein Levels in COVID-19 negative and positive intensive care unit patients: A prospective multicenter study","authors":"Vesile Örnek Diker, Gülseren Yılmaz, M. E. Düz, Mürvet Algemi, Mehmet Köseoğlu, Hümeyra Öztürk Emre, Osman Oğuz","doi":"10.5937/jomb0-47822","DOIUrl":"https://doi.org/10.5937/jomb0-47822","url":null,"abstract":"Background: \u0000Vitamin D binding protein plays a crucial role in regulating vitamin D levels by carrying vitamin D and its metabolites and immunological response by binding to endotoxins and fatty acids. We aimed to compare vitamin D, DBP, and specific inflammatory markers among intensive care unit (ICU) patients with and without the COVID-19 virus. \u0000 Methods: \u0000This multicenter study conducted in two training and research hospitals included 37 (13 female) COVID-19-positive and 51 (34 female) COVID-19-negative ICU patients. 25(OH) vitamin D, DBP, c-reactive protein (CRP), procalcitonin (PCT), D-dimer, troponin T (TnT), interleukin 6 (IL-6) and ferritin levels, survival, mortality rates, duration of stay (ICU) were examined. \u0000 Results: \u0000We observed higher ferritin and CRP levels, along with lower DBP, TnT and D-dimer levels, in patients with COVID-19. ICU patients with COVID-19 exhibited elevated mortality rates (Odds Ratio: 3.012, 95% Confidence Interval [1.252-7.248], p=0.013). However, there was no statistically significant correlation observed between mortality rates and Vitamin D or DBP levels across the entire ICU patient cohort. \u0000 Conclusions: \u0000Vitamin d values were found to be low in all intensive care patients, regardless of their covid 19 status. Contrary to the literature, COVID-19 patients had lower D-dimer and TNT levels than negative controls. However, COVID-19-positive ICU patients have decreased DBP. Further DBP gene polymorphisms studies are needed to explain this situation","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"24 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140082079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：