Introduction: The precision of test measurements is critical in clinical diagnostics, especially for biomarkers like total PSA and homocysteine, which are essential to disease assessment. Using the CMIA approach, this study investigates the repeatability and reproducibility of these biomarkers on the Abbott Alinity system. Materials and methods: The present study was conducted in the clinical chemistry laboratory at Mohammed VI University Hospital of Oujda. The evaluation of the Alinity i-system's analytical performance for total PSA and homocysteine focused on assessing repeatability and intermediate fidelity. The assessment followed the protocols and guidelines established by the French Society of Clinical Biology (SFBC). Results: Our analysis yielded favorable findings regarding the performance of the Alinity assays. The coefficients of variation for both the intra-assay and inter-assay precision were less than 5.89% and 4.29%, respectively. These findings underscore the tests' precision and consistency, affirming the CMIA method's reliability in accurately measuring total PSA and homocysteine levels. Conclusion: Our assessment of the Alinity i-system for total PSA and homocysteines showed significant analytical performance. Yet, limitations due to excluding critical parameters hindered a comprehensive evaluation. Addressing these in future investigations is crucial for upholding high diagnostic standards.
{"title":"Precision Matters: Repeatability and Reproducibility of Total PSA and Homocysteine Measurements in Alinity i-System","authors":"Oussama Grari","doi":"10.5937/jomb0-48306","DOIUrl":"https://doi.org/10.5937/jomb0-48306","url":null,"abstract":"Introduction: The precision of test measurements is critical in clinical diagnostics, especially for biomarkers like total PSA and homocysteine, which are essential to disease assessment. Using the CMIA approach, this study investigates the repeatability and reproducibility of these biomarkers on the Abbott Alinity system. \u0000Materials and methods: The present study was conducted in the clinical chemistry laboratory at Mohammed VI University Hospital of Oujda. The evaluation of the Alinity i-system's analytical performance for total PSA and homocysteine focused on assessing repeatability and intermediate fidelity. The assessment followed the protocols and guidelines established by the French Society of Clinical Biology (SFBC). \u0000Results: Our analysis yielded favorable findings regarding the performance of the Alinity assays. The coefficients of variation for both the intra-assay and inter-assay precision were less than 5.89% and 4.29%, respectively. These findings underscore the tests' precision and consistency, affirming the CMIA method's reliability in accurately measuring total PSA and homocysteine levels. \u0000 \u0000Conclusion: Our assessment of the Alinity i-system for total PSA and homocysteines showed significant analytical performance. Yet, limitations due to excluding critical parameters hindered a comprehensive evaluation. Addressing these in future investigations is crucial for upholding high diagnostic standards.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140425500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT Objective: In our study, we aimed to compare and validate the performance of the Friedewald, Martin/Hopkins, and Sampson formulae with direct LDL-C measurement. Material and Methods: The study was a retrospective investigation which by the Department of Medical Biochemistry of the Ankara Training and Research Hospital between 1 January 2021 and 31 December 2022. Our study evaluated the results of 6297 patients aged 18-99 years who underwent cholesterol panel TC, TG, HDL-C and direct LDL-C in our laboratory. Estimated LDL-C calculated according to Friedewald, Martin/Hopkins, and Sampson formulae. Results: All three formulae showed a stronger positive correlation with d-LDL-C (0.905, 0.897, and 0.886, respectively, for all data, p<0.001). In addition, when we compared the total median difference (1st-3rd quartile) of all formulae, it was it was -0.69 (-1.62-0.39) for Friedewald, 0.034 (-0.74 -1.14) for Martin/Hopkins and -0.40 (-1.19-0.55) for Sampson. According to Passing Bablok regression analyses, the intercept was determined as -0.97 (95% CI = -1.01 to -0.93), 0.41 (95%=0.37 to 0.44) and -0.05 (-0.08 to -0. 03) and slopes were calculated as 1.083 (95% CI = 1.07-1.09), 0.88 (0.88 to 0.89) and 0. 90 (95% = 0.89 to 0.90) for Friedewald, Martin/Hopkins and Sampson, respectively. Conclusions: Our findings suggest that Martin/Hopkins formula indicated a better performance than Friedewald and Sampson formulae. We figured out utilizing the Martin/Hopkins formula as a good alternative for estimated LDL-C in Turkish adults.
{"title":"Comparison of Friedewald, Martin/Hopkins, and Sampson formulae with direct LDL measurement in hyperlipidaemic and normolipidaemic adults","authors":"Medine Alpdemir","doi":"10.5937/jomb0-46549","DOIUrl":"https://doi.org/10.5937/jomb0-46549","url":null,"abstract":" \u0000ABSTRACT \u0000Objective: In our study, we aimed to compare and validate the performance of the Friedewald, Martin/Hopkins, and Sampson formulae with direct LDL-C measurement. \u0000Material and Methods: The study was a retrospective investigation which by the Department of Medical Biochemistry of the Ankara Training and Research Hospital between 1 January 2021 and 31 December 2022. Our study evaluated the results of 6297 patients aged 18-99 years who underwent cholesterol panel TC, TG, HDL-C and direct LDL-C in our laboratory. Estimated LDL-C calculated according to Friedewald, Martin/Hopkins, and Sampson formulae. \u0000Results: All three formulae showed a stronger positive correlation with d-LDL-C (0.905, 0.897, and 0.886, respectively, for all data, p<0.001). In addition, when we compared the total median difference (1st-3rd quartile) of all formulae, it was it was -0.69 (-1.62-0.39) for Friedewald, 0.034 (-0.74 -1.14) for Martin/Hopkins and -0.40 (-1.19-0.55) for Sampson. According to Passing Bablok regression analyses, the intercept was determined as -0.97 (95% CI = -1.01 to -0.93), 0.41 (95%=0.37 to 0.44) and -0.05 (-0.08 to -0. 03) and slopes were calculated as 1.083 (95% CI = 1.07-1.09), 0.88 (0.88 to 0.89) and 0. 90 (95% = 0.89 to 0.90) for Friedewald, Martin/Hopkins and Sampson, respectively. \u0000Conclusions: Our findings suggest that Martin/Hopkins formula indicated a better performance than Friedewald and Sampson formulae. We figured out utilizing the Martin/Hopkins formula as a good alternative for estimated LDL-C in Turkish adults. \u0000 ","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"2 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140434403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr sc. med. Violeta Rabrenović, Milica Petrović, M. Rabrenović, Nemanja Rančić
SUMMARY Background: Metabolic syndrome (MetS) in the patients with SLE represents an additional burden and a poor prognostic factor for the onset or worsening of atherosclerosis and cardiovascular complications. In many patients with lupus nephritis (LN) MetS is often already manifested initially. Our work aimed to determine the frequency and characteristics of MetS in patients with LN, as well as the relationship components of MetS and characteristics of disease activity Methods: The clinical study included 67 patients with LN, 54 (80.6%) female and 13 (19.4%) male, average age 42.86±14.46. Patients were divided into two groups: with MetS (35.8%) and without MetS (64.2%), active LN had (34 or 50.7%) and LN in remission (33 or 49.2%). We monitored clinical and biochemical parameters of interest. Results: Comparing patients with LN collectively, as well as those with MetS and without MetS, we observed that patients with MetS were older (p=0.001), BMI (p<0.001) and systolic arterial pressure was higher (p=0.002) and smokers were more common in this group (p<0.001). In the analysis, increased triglycerides (p<0.001) and creatinine (p=0.027), and decreased albumin (p=0.050) and GFR (p=0.020) were observed in the group with MetS. MetS was present in 44.1% of patients with active LN and in 27.7% with LN in remission. The most common MetS parameter was arterial hypertension (76.6%), which correlated with GFR and creatinine; hypertriglyceridemia (47.8%) which is correlated with anti ds DNA Ab, erythrocyturia, proteinuria and SLEDAI/r index; decreased HDL cholesterol (28.4%) which significantly correlated with albumin, C3 and anti ds DNA Ab. Conclusions: In our patients with LN, MetS was associated with older age, impair kidney function and smoking. The most common parameter of MetS was arterial hypertension and dyslipidemia, which were significantly correlated with disease activity parameters, indicating an increased risk of cardiovascular complications in this group of patients.
{"title":"METABOLIC SYNDROME IN PATIENTS WITH LUPUS NEPHRITIS","authors":"Dr sc. med. Violeta Rabrenović, Milica Petrović, M. Rabrenović, Nemanja Rančić","doi":"10.5937/jomb0-45732","DOIUrl":"https://doi.org/10.5937/jomb0-45732","url":null,"abstract":"SUMMARY \u0000Background: Metabolic syndrome (MetS) in the patients with SLE represents an additional burden and a poor prognostic factor for the onset or worsening of atherosclerosis and cardiovascular complications. In many patients with lupus nephritis (LN) MetS is often already manifested initially. Our work aimed to determine the frequency and characteristics of MetS in patients with LN, as well as the relationship components of MetS and characteristics of disease activity \u0000Methods: The clinical study included 67 patients with LN, 54 (80.6%) female and 13 (19.4%) male, average age 42.86±14.46. Patients were divided into two groups: with MetS (35.8%) and without MetS (64.2%), active LN had (34 or 50.7%) and LN in remission (33 or 49.2%). We monitored clinical and biochemical parameters of interest. \u0000Results: Comparing patients with LN collectively, as well as those with MetS and without MetS, we observed that patients with MetS were older (p=0.001), BMI (p<0.001) and systolic arterial pressure was higher (p=0.002) and smokers were more common in this group (p<0.001). In the analysis, increased triglycerides (p<0.001) and creatinine (p=0.027), and decreased albumin (p=0.050) and GFR (p=0.020) were observed in the group with MetS. MetS was present in 44.1% of patients with active LN and in 27.7% with LN in remission. The most common MetS parameter was arterial hypertension (76.6%), which correlated with GFR and creatinine; hypertriglyceridemia (47.8%) which is correlated with anti ds DNA Ab, erythrocyturia, proteinuria and SLEDAI/r index; decreased HDL cholesterol (28.4%) which significantly correlated with albumin, C3 and anti ds DNA Ab. \u0000Conclusions: In our patients with LN, MetS was associated with older age, impair kidney function and smoking. The most common parameter of MetS was arterial hypertension and dyslipidemia, which were significantly correlated with disease activity parameters, indicating an increased risk of cardiovascular complications in this group of patients. \u0000 \u0000 \u0000 ","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"61 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140436752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: This case report discusses how paraproteins interfere with multiple chemistry analyses and protocols to overcome such obstacles. Case Report: A serum specimen containing two monoclonal IgA (λ – light chain) paraproteins is subjected to a battery of tests on three wet chemistry platforms – AU5800, Cobas Pure and Alinity ci; results were compared with those on a Vitros 350/ ECiQ dry chemistry platform. Paraprotein interference was found to affect the bilirubins, inorganic phosphate and iron, whose repeat runs were also found to be irreproducible. Dilution with normal saline also failed to produce a satisfactory effect. Deproteinization by polyethylene glycol and dilution of the specimen with a normal serum specimen were observed to produce desirable results. Conclusion: Interference by IgA paraprotein on measurement of the bilirubins, phosphate and iron in the wet chemistry system can be mitigated either by deproteinization or by dilution with a normal serum.
{"title":"A Case of Atypical IgA Paraprotein Interference on Multiple Chemistry Assays: How to Deal with It","authors":"Rajarshi Sarkar","doi":"10.5937/jomb0-48289","DOIUrl":"https://doi.org/10.5937/jomb0-48289","url":null,"abstract":"\u0000Background: This case report discusses how paraproteins interfere with multiple chemistry analyses and protocols to overcome such obstacles. \u0000 \u0000 \u0000Case Report: A serum specimen containing two monoclonal IgA (λ – light chain) paraproteins is subjected to a battery of tests on three wet chemistry platforms – AU5800, Cobas Pure and Alinity ci; results were compared with those on a Vitros 350/ ECiQ dry chemistry platform. Paraprotein interference was found to affect the bilirubins, inorganic phosphate and iron, whose repeat runs were also found to be irreproducible. Dilution with normal saline also failed to produce a satisfactory effect. Deproteinization by polyethylene glycol and dilution of the specimen with a normal serum specimen were observed to produce desirable results. \u0000 \u0000 \u0000Conclusion: Interference by IgA paraprotein on measurement of the bilirubins, phosphate and iron in the wet chemistry system can be mitigated either by deproteinization or by dilution with a normal serum. \u0000","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"5 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139593259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Traceability is an important tool in the harmonization and standardization of reporting laboratory results, making them comparable across measurement systems. Driven by ISO 15189 accreditation requirements, medical laboratories have entered the era of metrological traceability. For medical laboratories, although calibrators are a key factor in the entire metrological traceability system, there is controversy over internal quality control (IQC) materials. It has been proposed that trueness materials supplied by the system's manufacturer with metrological traceability can be used for monitoring IQC so as to confirm that the performance of the measuring system is properly unbiased. This article discusses the pros, cons, and some myths regarding the new 2022 edition of ISO 15189 standard requirements for IQC and the application of traceability in daily operations. The discussion would focus on the implementation challenges and operational hurdles of applying traceability to IQC materials for trueness verification in medical laboratories. There are practical considerations with regard to the preparation of the IQC materials. Our suggestion is that testing of available different lot numbers of the manufacturer’s end-user calibrators on a routine basis, perhaps weekly or monthly, would be much more practical and feasible, instead of testing IQC materials with metrological traceability. We must acknowledge the limitations and restrictions that manufacturers and laboratories need to face before the recommendations can be applied in routine practices.
可追溯性是实现实验室结果报告统一化和标准化的重要工具，可使不同测量系统的结果具有可比性。在 ISO 15189 认证要求的推动下，医学实验室已进入计量溯源时代。对于医学实验室来说，虽然校准器是整个计量溯源系统的关键因素，但内部质量控制（IQC）材料却存在争议。有人提出，可使用由具有计量溯源性的系统制造商提供的真实性材料来监控 IQC，从而确认测量系统的性能是正确无偏的。本文将讨论新版 ISO 15189 2022 标准对 IQC 的要求以及在日常操作中应用溯源性的利弊和一些误解。讨论的重点是在医学实验室中将可追溯性应用于 IQC 材料真实性验证的实施挑战和操作障碍。在准备 IQC 材料方面有一些实际考虑因素。我们的建议是，对制造商的最终用户校准物的现有不同批号进行例行检测，也许每周或每月一次，这比检测具有计量溯源性的 IQC 材料更实际、更可行。我们必须承认，在将这些建议应用于常规实践之前，制造商和实验室需要面对各种限制和约束。
{"title":"Practical Considerations of Applying Traceability Concepts to the Internal Quality Control Materials for Trueness Verification in Medical Laboratories","authors":"Weizhi Cheng","doi":"10.5937/jomb0-45468","DOIUrl":"https://doi.org/10.5937/jomb0-45468","url":null,"abstract":"Traceability is an important tool in the harmonization and standardization of reporting laboratory results, making them comparable across measurement systems. Driven by ISO 15189 accreditation requirements, medical laboratories have entered the era of metrological traceability. For medical laboratories, although calibrators are a key factor in the entire metrological traceability system, there is controversy over internal quality control (IQC) materials. It has been proposed that trueness materials supplied by the system's manufacturer with metrological traceability can be used for monitoring IQC so as to confirm that the performance of the measuring system is properly unbiased. This article discusses the pros, cons, and some myths regarding the new 2022 edition of ISO 15189 standard requirements for IQC and the application of traceability in daily operations. The discussion would focus on the implementation challenges and operational hurdles of applying traceability to IQC materials for trueness verification in medical laboratories. There are practical considerations with regard to the preparation of the IQC materials. Our suggestion is that testing of available different lot numbers of the manufacturer’s end-user calibrators on a routine basis, perhaps weekly or monthly, would be much more practical and feasible, instead of testing IQC materials with metrological traceability. We must acknowledge the limitations and restrictions that manufacturers and laboratories need to face before the recommendations can be applied in routine practices.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139608582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrej Pešić, Vojin Vuković, Sofija Kozarac, V. Otašević, Tamara Bibić, B. Mihaljević, D. Antić
Hemophagocytic lymphohistiocytosis (HLH) is a rare, life-threatening hyperinflammatory disorder characterized by dysfunction of NK cells and cytotoxic lymphocytes. We present a rare case of a patient diagnosed with HLH who presented with persistent fever during treatment for refractory T-cell/histiocyte-rich large B-cell lymphoma (TCHRBCL), highlighting the challenges of managing HLH in the context of refractory lymphoma. According to our review of the literature, this is the first case of HLH that developed several months into treatment for refractory TCHRBCL and not in close temporal relation to lymphoma diagnosis.
嗜血细胞性淋巴组织细胞增多症（HLH）是一种罕见的、危及生命的高炎症性疾病，其特点是NK细胞和细胞毒性淋巴细胞功能失调。我们介绍了一例罕见的 HLH 患者，该患者在治疗难治性 T 细胞/富组织细胞大 B 细胞淋巴瘤（TCHRBCL）期间出现持续发热，这突显了在难治性淋巴瘤背景下处理 HLH 所面临的挑战。根据我们的文献综述，这是第一例在治疗难治性TCHRBCL数月后出现的HLH，与淋巴瘤诊断的时间关系并不密切。
{"title":"Unique presentation of T-cell/histiocyte-rich large B cell lymphoma complicated with hemophagocytic lymphohistiocytosis: case report and review of the literature","authors":"Andrej Pešić, Vojin Vuković, Sofija Kozarac, V. Otašević, Tamara Bibić, B. Mihaljević, D. Antić","doi":"10.5937/jomb0-48290","DOIUrl":"https://doi.org/10.5937/jomb0-48290","url":null,"abstract":"\u0000Hemophagocytic lymphohistiocytosis (HLH) is a rare, life-threatening hyperinflammatory disorder characterized by dysfunction of NK cells and cytotoxic lymphocytes. We present a rare case of a patient diagnosed with HLH who presented with persistent fever during treatment for refractory T-cell/histiocyte-rich large B-cell lymphoma (TCHRBCL), highlighting the challenges of managing HLH in the context of refractory lymphoma. According to our review of the literature, this is the first case of HLH that developed several months into treatment for refractory TCHRBCL and not in close temporal relation to lymphoma diagnosis.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"37 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139446349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saira Rafaqat, Irena Radoman Vujacic, Dimitrios Patoulias, Huma Khurshid, Aleksandra Klisic
Acute pancreatitis (AP) is characterized by an inflammatory response that leads to edema and haemorrhaging of pancreatic tissue. In severe cases, it can even result in the necrosis of pancreatic tissue following activation within the pancreas. Adipokines are biologically active molecules released by adipose tissue that have a wide-ranging impact on health and disease. Adipokines are cytokines produced not only in white adipose tissue but also in the fat surrounding the pancreas, and they play a role in the body's inflammatory response. The presence of increased adipose tissue, often associated with obesity, has been linked to a heightened systemic inflammatory response in cases of AP. According to the literature, there are many adipokines. This article summarizes the role of adipokines in AP. Adipokines could be the promising biomarkers for both diagnostic and new therapeutic treatment strategies in AP. However, a deeper knowledge of signaling pathways of adipokines and its potential therapeutic role in AP is necessary.
急性胰腺炎（AP）的特点是炎症反应导致胰腺组织水肿和出血。在严重的情况下，胰腺内的活化甚至会导致胰腺组织坏死。脂肪因子是由脂肪组织释放的生物活性分子，对健康和疾病有广泛的影响。脂肪因子是一种细胞因子，不仅在白色脂肪组织中产生，在胰腺周围的脂肪中也会产生，它们在机体的炎症反应中发挥作用。脂肪组织的增加（通常与肥胖有关）与 AP 病例中全身炎症反应的加剧有关。根据文献记载，有许多脂肪因子。本文总结了脂肪因子在 AP 中的作用。 脂肪因子可能是有希望成为 AP 诊断和新治疗策略的生物标志物。然而，有必要深入了解脂肪因子的信号通路及其在 AP 中的潜在治疗作用。
{"title":"Adipokines and their role in acute pancreatitis","authors":"Saira Rafaqat, Irena Radoman Vujacic, Dimitrios Patoulias, Huma Khurshid, Aleksandra Klisic","doi":"10.5937/jomb0-47515","DOIUrl":"https://doi.org/10.5937/jomb0-47515","url":null,"abstract":"Acute pancreatitis (AP) is characterized by an inflammatory response that leads to edema and haemorrhaging of pancreatic tissue. In severe cases, it can even result in the necrosis of pancreatic tissue following activation within the pancreas. Adipokines are biologically active molecules released by adipose tissue that have a wide-ranging impact on health and disease. Adipokines are cytokines produced not only in white adipose tissue but also in the fat surrounding the pancreas, and they play a role in the body's inflammatory response. The presence of increased adipose tissue, often associated with obesity, has been linked to a heightened systemic inflammatory response in cases of AP. According to the literature, there are many adipokines. This article summarizes the role of adipokines in AP. Adipokines could be the promising biomarkers for both diagnostic and new therapeutic treatment strategies in AP. However, a deeper knowledge of signaling pathways of adipokines and its potential therapeutic role in AP is necessary.","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"5 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139446957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Osteoporosis is characterized by low bone mass and altered bone microarchitecture. Patients with osteoporosis are at significantly increased risk for fragility fractures, which ultimately suffer fractures. The occurrence and development of osteoporotic fractures are significantly associated with high mortality, reduced quality of life as well as comorbidities. Biochemical indicators of bone metabolism are important for assessing the risk of fracture occurrence. In this study, we aimed to investigate the risk factors for osteoporotic fracture in the elderly based on bone metabolism biochemical indexes and to analyze their predictive efficacy through relevant bone metabolism biochemical indexes. Methods: A total of 254 elderly osteoporosis (OS) patients diagnosed and treated in our hospital during May 2019 to April 2022 was randomly picked, of which 100 patients were finally chosen for subsequent analysis following the inclusion and exclusion criteria. Patients were divided into OS fracture group and non-fracture group according to whether they had OS fracture. The contents of bone mineral density (BMD) and bone metabolism biochemical indexes, including Dickkopf-1 (DKK-1), sclerostin (SOST), osteoprotegerin (OPG), osteopontin (OPN), osteocalcin (BGP) and 25 hydroxyvitamin d (25 (OH) D) were detected in lumbar L2~4 and left femoral greater trochanter. The correlation between bone metabolism and BMD was evaluated using Pearson analysis. The risk factors of OS fracture were analyzed using Multivariate logistic regression analysis. The predictive value of biochemical indexes of bone metabolism on the risk of OS fracture was analyzed using ROC curve. Results: The proportion of patients with age and lack of sunlight in the OS fracture group was significantly higher than that in the non-fracture group (P < 0.05). The BMD in lumbar L2~4 and left femoral greater trochanter of patients in the OS fracture group was lower than that of patients in the non-fracture group (P < 0.05). At 14 weeks and 16 weeks after surgery, the levels of DKK-1, SOST and OPN in the OS fracture group were higher than these in the non-fracture group, and the levels of OPG, BGP and 25 (OH) D were lower than these in the non-fracture group (P < 0.05). BMD in lumbar L2~4, BMD in femoral greater trochanter, OPG, BGP and 25 (OH) D were the protective factors (P < 0.05), and the age, lack of sunlight, DKK-1, SOST and OPN were the risk factors affecting OS fractures (P < 0.05). BMD in lumbar L2~4 was negatively correlated with DKK-1, SOST and OPN (P < 0.05), and positively correlated with BGP and 25 (OH) D (P < 0.05). 25 (OH) D was positively correlated with femoral greater trochanter BMD (P < 0.05). OPG, OPN, BGP and 25 (OH) D had certain predictive value for the occurrence of OS fracture with the areas under the curve (AUC) of 0.709, 0.761, 0.720 and 0.730 respectively. The combined detection of all indicators had the AUC of 0.940 (P < 0.05), which had a high predictive valu
背景：骨质疏松症的特点是骨量低和骨微结构改变。骨质疏松症患者发生脆性骨折的风险明显增加，最终导致骨折。骨质疏松性骨折的发生和发展与高死亡率、生活质量下降以及合并症密切相关。骨代谢的生化指标对于评估骨折发生的风险非常重要。本研究旨在根据骨代谢生化指标调查老年人骨质疏松性骨折的风险因素，并通过相关骨代谢生化指标分析其预测功效。研究方法随机选取我院2019年5月至2022年4月期间诊治的老年骨质疏松症（OS）患者共254例，按照纳入和排除标准，最终选择其中100例患者进行后续分析。根据患者是否发生OS骨折分为OS骨折组和非骨折组。检测腰椎 L2~4 和左股骨大转子的骨矿物质密度（BMD）和骨代谢生化指标，包括 Dickkopf-1 (DKK-1)、硬骨素 (SOST)、骨保护素 (OPG)、骨生成素 (OPN)、骨钙素 (BGP) 和 25 羟维生素 d (25 (OH) D)。采用皮尔逊分析法评估了骨代谢与 BMD 之间的相关性。采用多变量逻辑回归分析法对 OS 骨折的风险因素进行了分析。采用 ROC 曲线分析骨代谢生化指标对 OS 骨折风险的预测价值。结果显示OS骨折组患者中年龄和缺乏日照的比例明显高于非骨折组（P<0.05）。OS 骨折组患者腰椎 L2~4 和左股骨大转子的 BMD 低于非骨折组患者（P < 0.05）。术后 14 周和 16 周，OS 骨折组 DKK-1、SOST 和 OPN 水平高于非骨折组，OPG、BGP 和 25 (OH) D 水平低于非骨折组（P < 0.05）。腰椎 L2~4 BMD、股骨大转子 BMD、OPG、BGP 和 25 (OH) D 是影响 OS 骨折的保护因素（P < 0.05），而年龄、缺乏日照、DKK-1、SOST 和 OPN 是影响 OS 骨折的危险因素（P < 0.05）。腰椎 L2~4 BMD 与 DKK-1、SOST 和 OPN 呈负相关（P < 0.05），与 BGP 和 25 (OH) D 呈正相关（P < 0.05）。25 (OH) D 与股骨大转子 BMD 呈正相关（P < 0.05）。OPG、OPN、BGP 和 25 (OH) D 对 OS 骨折的发生具有一定的预测价值，其曲线下面积（AUC）分别为 0.709、0.761、0.720 和 0.730。所有指标联合检测的 AUC 为 0.940（P < 0.05），对 OS 骨折具有较高的预测价值。结论骨代谢生化指标与 OS 骨折风险密切相关，作为 OS 骨折发生的影响因素，具有较高的预测价值。因此，准确地结合生化指标可降低老年人骨折的风险，从而有助于为老年骨折患者制定有针对性的治疗方案。
{"title":"Analysis of risk factors and predictive efficacy of senile osteoporosis fracture based on biochemical indicators of bone metabolism","authors":"Yufang Mao, Kanghua Li, Bing Zhu, Jiang Long","doi":"10.5937/jomb0-46663","DOIUrl":"https://doi.org/10.5937/jomb0-46663","url":null,"abstract":"Background: Osteoporosis is characterized by low bone mass and altered bone microarchitecture. Patients with osteoporosis are at significantly increased risk for fragility fractures, which ultimately suffer fractures. The occurrence and development of osteoporotic fractures are significantly associated with high mortality, reduced quality of life as well as comorbidities. Biochemical indicators of bone metabolism are important for assessing the risk of fracture occurrence. In this study, we aimed to investigate the risk factors for osteoporotic fracture in the elderly based on bone metabolism biochemical indexes and to analyze their predictive efficacy through relevant bone metabolism biochemical indexes. \u0000Methods: A total of 254 elderly osteoporosis (OS) patients diagnosed and treated in our hospital during May 2019 to April 2022 was randomly picked, of which 100 patients were finally chosen for subsequent analysis following the inclusion and exclusion criteria. Patients were divided into OS fracture group and non-fracture group according to whether they had OS fracture. The contents of bone mineral density (BMD) and bone metabolism biochemical indexes, including Dickkopf-1 (DKK-1), sclerostin (SOST), osteoprotegerin (OPG), osteopontin (OPN), osteocalcin (BGP) and 25 hydroxyvitamin d (25 (OH) D) were detected in lumbar L2~4 and left femoral greater trochanter. The correlation between bone metabolism and BMD was evaluated using Pearson analysis. The risk factors of OS fracture were analyzed using Multivariate logistic regression analysis. The predictive value of biochemical indexes of bone metabolism on the risk of OS fracture was analyzed using ROC curve. \u0000Results: The proportion of patients with age and lack of sunlight in the OS fracture group was significantly higher than that in the non-fracture group (P < 0.05). The BMD in lumbar L2~4 and left femoral greater trochanter of patients in the OS fracture group was lower than that of patients in the non-fracture group (P < 0.05). At 14 weeks and 16 weeks after surgery, the levels of DKK-1, SOST and OPN in the OS fracture group were higher than these in the non-fracture group, and the levels of OPG, BGP and 25 (OH) D were lower than these in the non-fracture group (P < 0.05). BMD in lumbar L2~4, BMD in femoral greater trochanter, OPG, BGP and 25 (OH) D were the protective factors (P < 0.05), and the age, lack of sunlight, DKK-1, SOST and OPN were the risk factors affecting OS fractures (P < 0.05). BMD in lumbar L2~4 was negatively correlated with DKK-1, SOST and OPN (P < 0.05), and positively correlated with BGP and 25 (OH) D (P < 0.05). 25 (OH) D was positively correlated with femoral greater trochanter BMD (P < 0.05). OPG, OPN, BGP and 25 (OH) D had certain predictive value for the occurrence of OS fracture with the areas under the curve (AUC) of 0.709, 0.761, 0.720 and 0.730 respectively. The combined detection of all indicators had the AUC of 0.940 (P < 0.05), which had a high predictive valu","PeriodicalId":504309,"journal":{"name":"Journal of Medical Biochemistry","volume":"21 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139385365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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