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Deciphering the Glycan Kryptos by Solid-State Nanopore Single-Molecule Sensing: A Call for Integrated Advancements Across Glyco- and Nanopore Science. 通过固态纳米孔单分子传感技术解密聚糖氪:呼吁综合推进糖和纳米孔科学。
Pub Date : 2024-05-10 DOI: 10.1149/2754-2726/ad49b0
Megan E. Kizer, Jason R. Dwyer
Glycans, or complex carbohydrates, are information-rich biopolymers critical to many biological processes and with considerable importance in pharmaceutical therapeutics. Our understanding, though, is limited compared to other biomolecules such as DNA and proteins. The greater complexity of glycan structure and the limitations of conventional chemical analysis methods hinder glycan studies. Auspiciously, nanopore single-molecule sensors—commercially available for DNA sequencing—hold great promise as a tool for enabling and advancing glycan analysis. We focus on two key areas to advance nanopore glycan characterization: molecular surface coatings to enhance nanopore performance including by molecular recognition, and high-quality glycan chemical standards for training.
聚糖或复合碳水化合物是一种信息丰富的生物聚合物,对许多生物过程至关重要,在药物治疗方面也具有相当重要的意义。不过,与 DNA 和蛋白质等其他生物大分子相比,我们对它们的了解还很有限。聚糖结构的复杂性和传统化学分析方法的局限性阻碍了聚糖研究。令人惊喜的是,纳米孔单分子传感器(可用于 DNA 测序的商用传感器)有望成为实现和推进聚糖分析的工具。我们将重点放在推进纳米孔聚糖表征的两个关键领域:通过分子识别等方式提高纳米孔性能的分子表面涂层,以及用于培训的高质量聚糖化学标准。
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引用次数: 0
Surface-display Techniques in Electrochemical Biosensor Designs for Health Monitoring 用于健康监测的电化学生物传感器设计中的表面显示技术
Pub Date : 2024-05-10 DOI: 10.1149/2754-2726/ad49af
Courtney J Weber, Megan D Whisonant, Olivia M Clay, Olja Simoska
Enzymatic and microbial electrochemical biosensors integrate enzymes and microorganisms as biological recognition elements into the sensor design and functionality. Enzyme-based sensors offer high sensitivity and selectivity for target analyte detection. However, these have limited stability necessary for continuous analyte monitoring. Contrarily, microbe-based electrochemical sensors provide a means for continuous analyte sensing but are associated with challenges related to analyte selectivity in complex samples. To address these limitations, surface-display methods, which bind enzymes to microbial surfaces, enhance biosensor selectivity and sensitivity. This perspective outlines the application of surface-display techniques, offering a promising avenue for health monitoring.
酶和微生物电化学生物传感器将酶和微生物作为生物识别元素融入传感器的设计和功能中。基于酶的传感器对目标分析物的检测具有高灵敏度和高选择性。然而,这些传感器的稳定性有限,无法对分析物进行连续监测。与此相反,基于微生物的电化学传感器提供了一种连续检测分析物的方法,但却面临着复杂样品中分析物选择性方面的挑战。为了解决这些局限性,表面展示法将酶与微生物表面结合,提高了生物传感器的选择性和灵敏度。本视角概述了表面显示技术的应用,为健康监测提供了一条前景广阔的途径。
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引用次数: 0
Quantum Biosensors: Principles and Applications in Medical Diagnostics 量子生物传感器:医学诊断原理与应用
Pub Date : 2024-05-07 DOI: 10.1149/2754-2726/ad47e2
Suparna Das, Hirak Mazumdar, Kamil Reza Khondakar, A. Kaushik, Yogendra Kumar Mishra
Originating at the intersection of physics and biosensing, quantum biosensors (QB) are transforming medical diagnostics and personalized medicine by exploiting quantum phenomena to amplify sensitivity, specificity, and detection speed compared to traditional biosensors. Their foundation lies in the fusion of biological entities like DNA, proteins, or enzymes with quantum sensors, which elicits discernible alterations in light emissions when interacting with sample molecules. Their prowess in identifying disease-linked biomarkers presents an avenue for early diagnoses of conditions like Alzheimer's and cancer. Beyond this, they enable real-time monitoring of treatment responses by capturing the dynamism of biomarkers, but QB still face challenges, such as issues of stability, reproducibility, and intricate quantum interactions. Moreover, seamless integration into prevailing diagnostic frameworks necessitates careful consideration. Looking ahead, the evolution of QB navigates uncharted territories. Innovations in fabrication techniques, interdisciplinary collaborations, and standardization protocols emerge as pivotal areas of exploration. This comprehensive discourse encapsulates QB's principles, diverse iterations, and burgeoning medical utilities. It delves into inherent challenges and limitations, shedding light on the potential trajectories of future research. As QB continues to evolve, its potential to redefine medical diagnostics becomes increasingly tangible. The saga of QB resonates with possibilities, poised to reshape the diagnostic landscape profoundly.
量子生物传感器(QB)起源于物理学和生物传感学的交叉学科,与传统生物传感器相比,它利用量子现象提高灵敏度、特异性和检测速度,正在改变医疗诊断和个性化医疗。量子传感器的基础在于将 DNA、蛋白质或酶等生物实体与量子传感器融合在一起,当与样本分子相互作用时,量子传感器的光发射会产生明显的变化。量子传感器在识别与疾病相关的生物标志物方面的优势为阿尔茨海默氏症和癌症等疾病的早期诊断提供了一条途径。除此之外,它们还能捕捉生物标志物的动态变化,从而实现对治疗反应的实时监测,但 QB 仍然面临着各种挑战,如稳定性、可重复性和复杂的量子相互作用等问题。此外,将其无缝整合到现有诊断框架中也需要慎重考虑。展望未来,QB 的发展还处于未知领域。制造技术、跨学科合作和标准化协议等方面的创新成为探索的关键领域。本报告全面论述了 QB 的原理、不同的迭代和蓬勃发展的医疗用途。它深入探讨了固有的挑战和局限性,揭示了未来研究的潜在轨迹。随着 QB 的不断发展,其重新定义医疗诊断的潜力也变得越来越明显。QB 的传奇故事蕴含着无限的可能性,有望深刻重塑诊断领域的格局。
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引用次数: 0
Electrochemical Bio-wearables for Cortisol Monitoring 用于皮质醇监测的电化学生物可穿戴设备
Pub Date : 2024-04-19 DOI: 10.1149/2754-2726/ad408e
Richa Pandey
Cortisol is a key biomarker for stress, and its measurement has historically relied on intrusive and sporadic techniques like blood or saliva samples. By offering continuous, non-invasive monitoring, electrochemical cortisol monitors: a relatively recent innovation—offer a revolutionary strategy. This viewpoint examines the development, underlying ideas, scientific developments, and possible uses of electrochemical cortisol bio-wearables. The significance of these tools for stress research, clinical application, and individualized healthcare is also highlighted.
皮质醇是压力的关键生物标志物,其测量历来依赖于血液或唾液样本等侵入性的零星技术。电化学皮质醇监测仪(一种相对较新的创新技术)通过提供连续、非侵入性的监测,提供了一种革命性的策略。这一观点探讨了电化学皮质醇生物可穿戴设备的发展、基本理念、科学发展和可能的用途。同时还强调了这些工具对于压力研究、临床应用和个性化医疗保健的重要意义。
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引用次数: 0
Enhancing Structural Integrity, Optical Properties, and Room Temperature Formaldehyde Sensing Through Optimized Spray Deposition Rates 通过优化喷涂沉积速率提高结构完整性、光学性能和室温甲醛传感能力
Pub Date : 2024-04-18 DOI: 10.1149/2754-2726/ad4045
D. Rajkumar, Umamahesvari Hemakumar
This study explores the impact of deposition rate on the properties of TiO2 thin films produced via spray pyrolysis, focusing on their application in gas sensors. The analysis covers structural, morphological, optical, and gas sensing characteristics of TiO2 films deposited at rates between 1 and 2.5 ml/min. Studies show optimizing TiO2 film deposition rates at 2 ml/min significantly enhances formaldehyde detection, improving selectivity and achieving a rapid response of 7.52 at 20 ppm concentration. This study underscores the pivotal role of deposition rate optimization in augmenting the gas-sensing efficacy of TiO2 films, particularly for formaldehyde detection at ambient conditions. Optimal deposition rates are instrumental in enhancing sensor performance. The synergistic application of XRD and Raman spectroscopy unequivocally confirmed the presence of the TiO2 anatase phase, which is of paramount significance in gas sensing applications. FESEM furnished high-resolution insights into the surface morphology, revealing a spherical architecture. Furthermore, UV-Vis spectroscopy was employed to assess the optical band gap of the films, which exhibited a decrement correlating with the rate of deposition. Notably, a deposition rate of 2 ml/min markedly improved the TiO2 films' sensing performance. These insights are critical for developing cost-effective, high-performance gas sensors for cutting-edge applications.
本研究探讨了沉积速率对喷雾热解法生产的二氧化钛薄膜特性的影响,重点是其在气体传感器中的应用。分析涵盖了以 1 至 2.5 毫升/分钟的速率沉积的二氧化钛薄膜的结构、形态、光学和气体传感特性。研究表明,以 2 毫升/分钟的速率优化二氧化钛薄膜沉积,可显著增强甲醛检测能力,提高选择性,并在 20 ppm 浓度时实现 7.52 的快速响应。这项研究强调了优化沉积速率在提高二氧化钛薄膜的气体传感功效方面的关键作用,尤其是在环境条件下的甲醛检测方面。最佳沉积速率有助于提高传感器性能。XRD 和拉曼光谱的协同应用明确证实了二氧化钛锐钛矿相的存在,这在气体传感应用中至关重要。FESEM 提供了对表面形态的高分辨率洞察,揭示了一种球形结构。此外,还利用紫外可见光谱评估了薄膜的光带隙,其衰减与沉积速率相关。值得注意的是,2 毫升/分钟的沉积速率明显提高了二氧化钛薄膜的传感性能。这些见解对于为尖端应用开发具有成本效益的高性能气体传感器至关重要。
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引用次数: 0
Perspective—Advances in Voltammetric Methods for the Measurement of Biomolecules 透视--用于测量生物分子的伏安法的进展
Pub Date : 2024-04-09 DOI: 10.1149/2754-2726/ad3c4f
Nadiah M. Alyamni, J. Abot, A. Zestos
Voltammetry is a powerful electroanalytical tool that makes fast, real-time measurements of neurotransmitters and other molecules. Electroanalytical methods like cyclic, pulse, and stripping voltammetry are useful for qualitative and quantitative examination. Neurochemical sensing has been enhanced using carbon-based electrodes and waveform modification methods that improve sensitivity and stability of electrode performance. Voltammetry has revolutionized neurochemical monitoring by providing real-time information on neurotransmitter dynamics for neurochemical studies. Selectivity and electrode fouling remain issues for biomolecule detection, but recent advances promise new methods of analysis for other applications to enhance spatiotemporal resolution, sensitivity, selectivity, and other important considerations.
伏安法是一种强大的电分析工具,可对神经递质和其他分子进行快速、实时的测量。循环、脉冲和剥离伏安法等电分析方法可用于定性和定量检测。使用碳基电极和波形修改方法可提高灵敏度和电极性能的稳定性,从而增强神经化学传感。伏安法为神经化学研究提供了神经递质动态的实时信息,从而彻底改变了神经化学监测方法。选择性和电极堵塞仍然是生物大分子检测的问题,但最近的进展有望为其他应用提供新的分析方法,以提高时空分辨率、灵敏度、选择性和其他重要的考虑因素。
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引用次数: 0
Nonenzymatic Detection of Glucose Using 3D Printed Carbon Electrodes in Human Saliva 利用 3D 打印碳电极对人体唾液中的葡萄糖进行非酶检测
Pub Date : 2024-04-04 DOI: 10.1149/2754-2726/ad3a58
Dalton L. Glasco, Manar M. Elhassan, William T. McLeod, Jeffrey G. Bell
One of the most prevalent diseases where point-of-care (POC) diagnostics has focused is diabetes, which impacts hundreds of millions of people globally. Due to the severe negative outcomes including renal failure, nerve damage, and stroke, many POC sensors have been designed to streamline low-cost testing. Recently, the utility of 3D printing for rapidly fabricating housings, electrodes, and sensors for use at the POC has been exploited toward diverse applications. Particularly interesting are 3D printed carbon electrodes (3DpCEs) in POC diagnostics owing to their simplicity, affordability, and mass production capabilities for developing sensors either for direct use or through post-printing surface modifications. Herein, we report a copper modified 3DpCE as a sensitive and selective nonenzymatic biosensor for glucose. Copper deposition, paired with an optimized activation protocol, produced a sensitive and selective sensor for glucose with a larger detection range, enhanced sensitivity, and better reproducibility compared to nonactivated and alkaline immersed 3DpCEs. The sensor displayed excellent linearity between 10-1800 µM and proved to be highly selective over common biologically relevant interferants. The 3D printed sensor successfully determined biologically relevant concentrations of glucose in human saliva which resulted in percent recoveries of 101+8%, 106+6%, and 98+6% for 74, 402, and 652 µM glucose, respectively
糖尿病是护理点(POC)诊断技术最常见的疾病之一,影响着全球数亿人。由于糖尿病会导致严重的不良后果,包括肾功能衰竭、神经损伤和中风,许多 POC 传感器都是为了简化低成本检测而设计的。最近,3D 打印技术在快速制造 POC 用外壳、电极和传感器方面的效用已被广泛应用。在 POC 诊断中,3D 打印碳电极(3DpCE)因其简易性、经济性和批量生产能力而尤其引人关注,可用于开发直接使用或通过打印后表面改性使用的传感器。在此,我们报告了一种铜修饰的 3DpCE 作为葡萄糖的灵敏和选择性非酶生物传感器。铜沉积与优化的活化方案相结合,产生了一种灵敏且具有选择性的葡萄糖传感器,与未活化和碱性浸泡的 3DpCE 相比,其检测范围更大,灵敏度更高,重现性更好。该传感器在 10-1800 µM 之间显示出极佳的线性,并证明对常见的生物相关干扰物具有高度选择性。3D 打印传感器成功测定了人体唾液中葡萄糖的生物相关浓度,对 74、402 和 652 µM 葡萄糖的回收率分别为 101+8%、106+6% 和 98+6
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引用次数: 0
Review—Catalytic Electrochemical Biosensors for Dopamine: Design, Performance, and Healthcare Applications 评论-多巴胺催化电化学生物传感器:设计、性能和医疗应用
Pub Date : 2024-04-01 DOI: 10.1149/2754-2726/ad3950
Emily DeVoe, Silvana Andreescu
The effect of an Al2TiO5 (ALT) interlayer between Ni and YSZ on enhancing the thermal stability of Ni-YSZ solid oxide fuel cell was examined. Atomic layer deposition (ALD) was used to provide precise control of the structure and thickness of the ALT interlayer. The study’s findings demonstrate that a 2 nm thick ALT interlayer deposited by ALD does not adversely affect the cell’s ohmic resistance and effectively prevents Ni sintering and the loss of active area during high-temperature heat treatments. ALT layers thicker than 2 nm, although they enhanced Ni stability, were found to impede oxygen ion transport in the electrode and significantly increase the ohmic resistance of the cell, leading to a decline in performance.
研究了镍和 YSZ 之间的 Al2TiO5(ALT)夹层对提高镍-YSZ 固体氧化物燃料电池热稳定性的影响。原子层沉积 (ALD) 技术可精确控制 ALT 中间膜的结构和厚度。研究结果表明,通过 ALD 沉积的 2 nm 厚的 ALT 中间层不会对电池的欧姆电阻产生不利影响,并能有效防止高温热处理过程中的镍烧结和活性面积损失。厚度大于 2 纳米的 ALT 层虽然提高了镍的稳定性,但会阻碍电极中的氧离子传输,显著增加电池的欧姆电阻,导致性能下降。
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引用次数: 0
Review—Smart Wearable Sensors for Health and Lifestyle Monitoring: Commercial and Emerging Solutions 评论-用于健康和生活方式监测的智能可穿戴传感器:商业和新兴解决方案
Pub Date : 2024-03-19 DOI: 10.1149/2754-2726/ad3561
Niamh Marie Cusack, Prabhuraj Venkatraman, Umar Raza, Azmy Faisal
The rapid growth of urbanisation has brought about various health concerns for citizens living in urban environments. Sedentary lifestyles, increased pollution levels, and high levels of stress have become prevalent issues affecting the overall well-being of urban populations. In recent years, the emergence of smart wearable devices has offered a promising avenue to address these health concerns and promote healthier lifestyles. This review evaluatse the effectiveness of smart wearables in mitigating health concerns and improving the lifestyles of urban citizens. The review involves 50 relevant peer-reviewed smart wearable studies and supporting literature from electronic databases PubMed, Ovid, Web of Science, and Scopus. Results indicate that smart wearables have the potential to positively impact the health of urban citizens by promoting physical activity, tracking vital signs, monitoring sleep patterns, and providing personalised feedback and recommendations to promote physical activity levels. Furthermore, these devices can help individuals manage stress levels, enhance self-awareness, and foster healthier behaviours. However, the review also identifies several challenges, including the accuracy and reliability of wearable data, user engagement and adherence, and ethical considerations regarding data privacy and security.
城市化的快速发展给生活在城市环境中的市民带来了各种健康问题。久坐不动的生活方式、日益严重的污染和高度的压力已成为影响城市人口整体健康的普遍问题。近年来,智能可穿戴设备的出现为解决这些健康问题和促进更健康的生活方式提供了一条大有可为的途径。本综述评估了智能可穿戴设备在减轻健康问题和改善城市居民生活方式方面的有效性。综述涉及 50 项经同行评审的相关智能可穿戴设备研究,以及来自电子数据库 PubMed、Ovid、Web of Science 和 Scopus 的辅助文献。结果表明,智能可穿戴设备有可能通过促进体育锻炼、跟踪生命体征、监测睡眠模式以及提供个性化反馈和建议来提高体育锻炼水平,从而对城市居民的健康产生积极影响。此外,这些设备还能帮助个人管理压力水平、增强自我意识并促进更健康的行为。不过,综述也指出了一些挑战,包括可穿戴设备数据的准确性和可靠性、用户参与度和依从性,以及有关数据隐私和安全的伦理考虑。
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引用次数: 0
Fabrication of Highly Sensitive YCeO Chemo-resistive Gas Sensor for Selective Detection of CO2 制造用于选择性检测二氧化碳的高灵敏度 YCeO 化学电阻式气体传感器
Pub Date : 2024-03-13 DOI: 10.1149/2754-2726/ad33a3
Shivangi Srivastava, Narendra Kumar Pandey, V. Verma, Peramjeet Singh, Amit Verma, Neetu Yadav
A room-temperature-operated CO2 gas sensor based on YCeO nanocomposite was effectively prepared by the simple hydrothermal technique to detect low traces of CO2 (50-250 ppm). The YCeO granular morphological features were observed using field-emission scanning electron microscopy, which confirmed successful fabrication of nanocomposite of Y2O3 and CeO2. X-ray diffraction of YCeO showed the Cubic structure of space group Fm3m having density 6.74 gmcm-3. Rietveld refinement was performed for the analysis of complete crystal structural property. Surface porosity and specific surface area were observed by Brunnauer-Emmet Teller analysis. Optical properties were observed using UV-Visible spectroscopy. The band gap, optical conductivity, and refractive index calculated were 3.44 eV, 2.63×106, and 0.1164, respectively. Fourier transform infrared spectroscopy was done to analyze the functional and elastic properties of as-prepared nanomaterial. The highest sensor response recorded was 2.14. The response and recovery time at 50 ppm observed were 75.6 and 107.3 s, respectively. The YCeO chemo-resistive sensor confirmed long-term stability and selectivity to CO2 as compared to other gases viz. LPG, NH3, CH4, H2S, NO2 and H2. The relative humidity exposure was also performed at 15, 55 and 95% RH, in which it was confirmed that the sensor would give best response at mid humidity level i.e. 55 %RH. Sensing characteristics curve of YCeO nanocomposite at different temperature (30-90°C) at 50 ppm confirmed that YCeO sensor performed excellent at room temperature. This report unlocks an innovative opening for the fabrication of sensing devices that are room-temperature-operatable, highly-sensitive and selective for quick detection of CO2 gas for its commercialization
通过简单的水热技术有效地制备了一种基于 YCeO 纳米复合材料的室温操作二氧化碳气体传感器,可检测低痕量的二氧化碳(50-250 ppm)。利用场发射扫描电子显微镜观察到了 YCeO 颗粒的形态特征,证实了 Y2O3 和 CeO2 纳米复合材料的成功制备。YCeO 的 X 射线衍射显示其为立方结构,空间群为 Fm3m,密度为 6.74 gmcm-3。为了分析完整的晶体结构特性,对其进行了里特维尔德细化。通过布鲁瑙尔-艾美特-特勒分析法观察了表面孔隙率和比表面积。使用紫外-可见光谱观察了光学特性。计算得出的带隙、光导率和折射率分别为 3.44 eV、2.63×106 和 0.1164。傅立叶变换红外光谱分析了制备的纳米材料的功能和弹性特性。记录到的最高传感器响应为 2.14。在 50 ppm 时的响应和恢复时间分别为 75.6 秒和 107.3 秒。与 LPG、NH3、CH4、H2S、NO2 和 H2 等其他气体相比,YCeO 化学电阻传感器具有长期稳定性和对 CO2 的选择性。此外,还在 15%、55% 和 95% 相对湿度下进行了相对湿度曝露,结果表明传感器在中等湿度(即 55%相对湿度)下的反应最佳。YCeO 纳米复合材料在不同温度(30-90°C)下的传感特性曲线(50 ppm)证实,YCeO 传感器在室温下表现出色。该报告为制造可在室温下操作、高灵敏度和高选择性的传感设备开辟了一条创新之路,可用于快速检测二氧化碳气体,并将其商业化。
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引用次数: 0
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