The translational stem cell research field has progressed immensely in the past decade. Development and refinement of differentiation protocols now allows the generation of a range of cell types, such as pancreatic β-cells and dopaminergic neurons, from human pluripotent stem cells (hPSCs) in an efficient and good manufacturing practice-compliant fashion. This has led to the initiation of several clinical trials using hPSC-derived cells to replace lost or dysfunctional cells, demonstrating evidence of both safety and efficacy. Here, we highlight successes from some of the hPSC-based trials reporting early signs of efficacy and discuss common challenges in clinical translation of cell therapies.
{"title":"Clinical translation of pluripotent stem cell-based therapies: successes and challenges.","authors":"Josefine Rågård Christiansen, A. Kirkeby","doi":"10.1242/dev.202067","DOIUrl":"https://doi.org/10.1242/dev.202067","url":null,"abstract":"The translational stem cell research field has progressed immensely in the past decade. Development and refinement of differentiation protocols now allows the generation of a range of cell types, such as pancreatic β-cells and dopaminergic neurons, from human pluripotent stem cells (hPSCs) in an efficient and good manufacturing practice-compliant fashion. This has led to the initiation of several clinical trials using hPSC-derived cells to replace lost or dysfunctional cells, demonstrating evidence of both safety and efficacy. Here, we highlight successes from some of the hPSC-based trials reporting early signs of efficacy and discuss common challenges in clinical translation of cell therapies.","PeriodicalId":505872,"journal":{"name":"Development","volume":"744 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140772939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Nashchekin, I. Squires, A. Prokop, D. St Johnston
In Drosophila, only one cell in a multicellular female germline cyst is specified as an oocyte and a similar process occurs in mammals. The symmetry-breaking cue for oocyte selection is provided by the fusome, a tubular structure connecting all cells in the cyst. The Drosophila spectraplakin Shot localises to the fusome and translates its asymmetry into a polarised microtubule network that is essential for oocyte specification, but how Shot recognises the fusome is unclear. Here, we demonstrate that the actin-binding domain (ABD) of Shot is necessary and sufficient to localise Shot to the fusome and mediates Shot function in oocyte specification together with the microtubule-binding domains. The calponin homology domain 1 of the Shot ABD recognises fusomal F-actin and requires calponin homology domain 2 to distinguish it from other forms of F-actin in the cyst. By contrast, the ABDs of utrophin, Fimbrin, Filamin, Lifeact and F-tractin do not recognise fusomal F-actin. We therefore propose that Shot propagates fusome asymmetry by recognising a specific conformational state of F-actin on the fusome.
{"title":"The Shot CH1 domain recognises a distinct form of F-actin during Drosophila oocyte determination.","authors":"D. Nashchekin, I. Squires, A. Prokop, D. St Johnston","doi":"10.1242/dev.202370","DOIUrl":"https://doi.org/10.1242/dev.202370","url":null,"abstract":"In Drosophila, only one cell in a multicellular female germline cyst is specified as an oocyte and a similar process occurs in mammals. The symmetry-breaking cue for oocyte selection is provided by the fusome, a tubular structure connecting all cells in the cyst. The Drosophila spectraplakin Shot localises to the fusome and translates its asymmetry into a polarised microtubule network that is essential for oocyte specification, but how Shot recognises the fusome is unclear. Here, we demonstrate that the actin-binding domain (ABD) of Shot is necessary and sufficient to localise Shot to the fusome and mediates Shot function in oocyte specification together with the microtubule-binding domains. The calponin homology domain 1 of the Shot ABD recognises fusomal F-actin and requires calponin homology domain 2 to distinguish it from other forms of F-actin in the cyst. By contrast, the ABDs of utrophin, Fimbrin, Filamin, Lifeact and F-tractin do not recognise fusomal F-actin. We therefore propose that Shot propagates fusome asymmetry by recognising a specific conformational state of F-actin on the fusome.","PeriodicalId":505872,"journal":{"name":"Development","volume":"41 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140783668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-10DOI: 10.1057/s41301-023-00399-8
Nicoletta Dentico
{"title":"Editorial: One Health—Anatomy of a Fractured Vision","authors":"Nicoletta Dentico","doi":"10.1057/s41301-023-00399-8","DOIUrl":"https://doi.org/10.1057/s41301-023-00399-8","url":null,"abstract":"","PeriodicalId":505872,"journal":{"name":"Development","volume":"85 2","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139440737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-11DOI: 10.1057/s41301-020-00252-2
Aidan Eyakuze, Khalifa Said
{"title":"The Weaponization of Identity and Citizenship: The Case of Tanzania","authors":"Aidan Eyakuze, Khalifa Said","doi":"10.1057/s41301-020-00252-2","DOIUrl":"https://doi.org/10.1057/s41301-020-00252-2","url":null,"abstract":"","PeriodicalId":505872,"journal":{"name":"Development","volume":"21 15","pages":"95 - 99"},"PeriodicalIF":0.0,"publicationDate":"2020-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141226519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-05DOI: 10.1057/s41301-020-00250-4
{"title":"Archeology of Knowledge: An Interview with Heidi Grunebaum on Identities and Citizenship","authors":"","doi":"10.1057/s41301-020-00250-4","DOIUrl":"https://doi.org/10.1057/s41301-020-00250-4","url":null,"abstract":"","PeriodicalId":505872,"journal":{"name":"Development","volume":"10 46","pages":"100 - 105"},"PeriodicalIF":0.0,"publicationDate":"2020-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141226398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-27DOI: 10.1057/s41301-020-00236-2
Zainab Najeeb
{"title":"The Soldier, The Terrorist, and The Woman: A Gendered Analysis of Enforced Displacement in Northwestern Pakistan Post 2014","authors":"Zainab Najeeb","doi":"10.1057/s41301-020-00236-2","DOIUrl":"https://doi.org/10.1057/s41301-020-00236-2","url":null,"abstract":"","PeriodicalId":505872,"journal":{"name":"Development","volume":"22 2","pages":"83 - 89"},"PeriodicalIF":0.0,"publicationDate":"2020-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141226512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-15DOI: 10.1057/s41301-020-00242-4
C. Grigore
{"title":"Closing the Gap Between Legal and Social Citizenship for Roma People","authors":"C. Grigore","doi":"10.1057/s41301-020-00242-4","DOIUrl":"https://doi.org/10.1057/s41301-020-00242-4","url":null,"abstract":"","PeriodicalId":505872,"journal":{"name":"Development","volume":"5 3","pages":"6 - 8"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141227675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-14DOI: 10.1057/s41301-020-00234-4
A. Zia
{"title":"The Haunting Specter of Hindu Ethnonationalist-Neocolonial Development in the Indian Occupied Kashmir","authors":"A. Zia","doi":"10.1057/s41301-020-00234-4","DOIUrl":"https://doi.org/10.1057/s41301-020-00234-4","url":null,"abstract":"","PeriodicalId":505872,"journal":{"name":"Development","volume":"23 3","pages":"60 - 66"},"PeriodicalIF":0.0,"publicationDate":"2020-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141226326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-05DOI: 10.1057/s41301-019-00232-1
Nicole S. Marshall
{"title":"Climate Migration and Loss: Exploring the Conceptual Borders of Citizenship, Sovereign Authority, and the Deterritorialized State","authors":"Nicole S. Marshall","doi":"10.1057/s41301-019-00232-1","DOIUrl":"https://doi.org/10.1057/s41301-019-00232-1","url":null,"abstract":"","PeriodicalId":505872,"journal":{"name":"Development","volume":"8 24","pages":"20 - 26"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141226122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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