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The role of the forensic archeologist and anthropologist in recovery of human remains from fatal fires 法医考古学家和人类学家在从致命火灾中找回遗骸方面的作用
Pub Date : 2024-07-24 DOI: 10.1002/wfs2.1529
Diana Swales, Lucina Hackman
Fatal fires pose complex challenges for responders due to the requirement to investigate all aspects of the fire using methods that maximize evidence recovery and integrity, including optimal and respectful recovery of the deceased. In this article, the authors consider the value of the inclusion of both forensic archeologists and forensic anthropologists in circumstances of fatal fire, identifying some of the challenges posed by these environments and what each can contribute.This article is categorized under:Crime Scene Investigation > Crime Scene ExaminationForensic Anthropology > Forensic ArchaeologyForensic Anthropology > Taphonomic Changes and the Environment
致命火灾给救灾人员带来了复杂的挑战,因为他们需要使用能够最大限度地恢复证据和证据完整性的方法对火灾的各个方面进行调查,包括以最佳和尊重死者的方式进行恢复。在这篇文章中,作者考虑了法医考古学家和法医人类学家共同参与致命火灾环境调查的价值,确定了这些环境带来的一些挑战以及他们各自可以做出的贡献。本文所属分类:犯罪现场调查 > 犯罪现场勘查法医人类学 > 法医考古学法医人类学 > 岩石学变化与环境
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引用次数: 0
Cognitive bias and contextual information management: Considerations for forensic handwriting examinations 认知偏差和上下文信息管理:法医笔迹鉴定的注意事项
Pub Date : 2024-07-23 DOI: 10.1002/wfs2.1530
Carolyne Bird, Kylie Jones, K. Ballantyne
As for other largely subjective perceptual and cognitive processes, the forensic analysis and interpretation of handwriting is potentially susceptible to bias. Cognitive bias may subconsciously influence the outcome of specific tasks at any point in an examination, during pre‐analysis, analysis, comparison, interpretation and/or reporting of results. One class of cognitive bias, contextual bias, may arise from information provided by the client, results reported from other forensic disciplines within a laboratory or the content of the questioned and/or known material itself. The base rate information (i.e., examiner knowledge, experience, and innate beliefs) associated with the type and range of examinations an examiner has previously encountered may also lead to bias. Bias introduced at any stage of the examination process, and even prior to the examination (e.g., when deciding what is collected for examination) may affect the examination outcome.This Overview focuses on contextual information management (CIM) protocols that are used to remove potentially biasing task‐irrelevant case information from the view of the reporting forensic handwriting examiner (FHE). Where laboratory resources and logistics allow for these procedures to be in place, they should be applied as part of the pre‐analysis stage of the examination process that is, at a stage in the examination prior to the reporting FHE accessing the examination material or associated documentation. Also highlighted are some additional procedures that have been proposed by others to minimize the opportunity for cognitive bias to arise in handwriting examinations.This article is categorized under:Jurisprudence and Regulatory Oversight > Expert Evidence and Narrative
与其他主观性很强的感知和认知过程一样,笔迹的法医分析和解释也有可能出现偏差。在检查的任何阶段,在分析前、分析、比较、解释和/或报告结果的过程中,认知偏差都可能潜移默化地影响特定任务的结果。一类认知偏差,即背景偏差,可能来自客户提供的信息、实验室内其他法医学科报告的结果或被质疑和/或已知材料本身的内容。与检查员以前遇到的检查类型和范围相关的基础率信息(即检查员的知识、经验和固有信念)也可能导致偏差。在检查过程的任何阶段,甚至是在检查之前(例如,在决定收集哪些信息进行检查时)引入的偏见都可能影响检查结果。本概述重点介绍背景信息管理(CIM)协议,这些协议用于从报告法医笔迹检查员(FHE)的视线中移除可能存在偏见的与任务无关的案件信息。在实验室资源和后勤条件允许的情况下,这些程序应作为检查流程分析前阶段的一部分来使用,即在报告法医笔迹检验员(FHE)获取检验材料或相关文件之前的检查阶段使用。本文归类于:法理学与法规监督 > 专家证据与叙述。
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引用次数: 0
Development of human remains investigations at fatal fire scenes: Bridging anthropology and fire sciences in the United States 在致命火灾现场开展人类遗骸调查:美国人类学与消防科学的桥梁
Pub Date : 2024-07-21 DOI: 10.1002/wfs2.1527
Hanna Friedlander, Jaymelee J. Kim, Joe Allen
Anthropological assessment of a scene for fatal fire victims can prove crucial in the search, recovery and identification of human remains from fire‐related death investigations. On scene contributions of the anthropologist include, but are not limited to, searching for remains in burnt structures, vehicles, roadside dumps, campfire accidents, and other fire investigations, as well as the proper documentation and collection of remains. While patterning and sequencing for all fire events is not standardized, thermal alteration can be classified and consistent terminology can be used to reflect commonalities and evaluation of thermally altered remains. Further, the anthropologist has the ability to evaluate damage to the remains in terms of possible associated trauma (e.g., antemortem, postmortem). However, scene investigators do not regularly call upon anthropologists to assist at the scene and often do not know the contributions and benefits of having an anthropologist on scene. This perspective article focuses on the development of fatal fire investigations within the United States, key terminology, and core concepts within anthropological sciences and provides recommendations for how fire investigators and anthropologists can collaborate–bridging the gap between the two fields. Uniting literature from fire science, anthropology, and fire investigations, this overview underscores the importance of anthropology and fire investigator collaboration.This article is categorized under:Forensic Anthropology > Taphonomic Changes and the EnvironmentForensic Anthropology > Trauma AnalysisForensic Anthropology > Anthropology in Mass Disaster & War Crime Contexts
对火灾现场致命受害者的人类学评估对于搜索、恢复和鉴定与火灾有关的死亡调查中的遗骸至关重要。人类学家在现场的工作包括但不限于在被烧毁的建筑物、车辆、路边垃圾堆、篝火事故和其他火灾调查中搜寻遗骸,以及妥善记录和收集遗骸。虽然所有火灾事件的模式和顺序没有统一标准,但可以对热改变进行分类,并使用一致的术语来反映热改变遗骸的共性和评估。此外,人类学家有能力根据可能的相关创伤(如死前、死后)来评估遗骸的损坏情况。然而,现场调查人员并不经常要求人类学家到现场提供协助,他们往往不知道人类学家在现场的贡献和好处。这篇透视文章重点介绍了美国致命火灾调查的发展、关键术语以及人类学科学中的核心概念,并就火灾调查人员和人类学家如何合作缩小两个领域之间的差距提出了建议。这篇综述结合了火灾科学、人类学和火灾调查的文献,强调了人类学和火灾调查人员合作的重要性。本文被归类为:法医人类学 > 岩石学变化与环境法医人类学 > 创伤分析法医人类学 > 大规模灾难和战争犯罪背景下的人类学。
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引用次数: 0
Forensic intelligence: Expanding the potential of forensic document examination 法证情报:扩大法证文件检查的潜力
Pub Date : 2024-07-10 DOI: 10.1002/wfs2.1528
Ciara Devlin, M. Morelato, Simon Baechler
Forensic document examination is characterized by its longevity, diversity, and evolution over time. Predominantly, published research within this field has focused on handwriting examination, the articulation of forensic conclusions, and the development of technical instrumental advancements, focusing on the use of document examination in the resolution of casework. This is a persistent and common problem within forensic science that Kirk identified in 1963 and that other authors have reaffirmed more recently. Ultimately, this has resulted in the potential of forensic intelligence, remaining relatively underexplored in the field of document examination. Forensic intelligence is a different way to view and analyze traces, shifting the focus from the traditional identification of source and activity, to instead identifying trends in criminal activity to assist in the reduction, prevention, and proactive disruption of crime. Despite a distinct disparity between these strands of research, there has been a persevering evolution toward the implementation of a systematic forensic intelligence method for the examination of fraudulent identity documents. Since its initial inception into the research community, this method has expanded and been implemented across Europe, and Canada, with tests also being conducted in Australia. These first tangible steps toward a forensic intelligence capacity within document examination have also inspired new work using forensic intelligence and systematic comparisons within the field of handwriting examination, as well as the recognition of the transversal potential of this method, with it being applied to both physical and digital documents. In this review, the fields of document examination and forensic intelligence will first be introduced, along with a subsequent examination of the research that has led to the creation of a forensic intelligence model within the field of document examination. It should be noted that this review has largely been limited to a review of research that has been published in English and French due to the language of the authors.This article is categorized under:Crime Scene Investigation > From Traces to Intelligence and EvidenceForensic Chemistry and Trace Evidence > Emerging Technologies and MethodsCrime Scene Investigation > Epistemology and Method
法证文件检验的特点是长期性、多样性和随时间的演变。该领域已发表的研究主要集中在笔迹检验、法医结论的表述和技术工具的发展进步,重点是在解决案件工作中使用文件检验。这是法医学中一个长期存在的普遍问题,科克(Kirk)早在 1963 年就指出了这一问题,其他作者最近也重申了这一问题。最终,这导致法证情报的潜力在文件检验领域仍未得到充分发掘。法证情报是一种看待和分析痕迹的不同方式,它将重点从传统的识别来源和活动转移到识别犯罪活动的趋势,以协助减少、预防和主动破坏犯罪。尽管这些研究领域之间存在着明显的差异,但人们一直在坚持不懈地朝着采用系统的法证情报方法来检查欺诈性身份证件的方向发展。这种方法自最初进入研究界以来,已在欧洲和加拿大得到推广和实施,澳大利亚也在进行测试。这些在文件检验中实现法证情报能力的具体步骤也激发了在笔迹检验领域使用法证情报和系统比较的新工作,同时也使人们认识到这种方法的横向潜力,它既可应用于实体文件,也可应用于数字文件。在本综述中,将首先介绍文件检验和法证情报领域,然后对导致在文件检验领域创建法证情报模型的研究进行审查。值得注意的是,由于作者语言的原因,本综述主要限于对以英语和法语发表的研究的综述。本文归类于:犯罪现场调查 > 从痕迹到情报和证据法证化学和痕迹证据 > 新兴技术和方法犯罪现场调查 > 认识论和方法
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引用次数: 0
Collaborative fire research program using donated human remains 利用捐赠遗骸开展火灾研究合作计划
Pub Date : 2024-07-04 DOI: 10.1002/wfs2.1526
Rebecca L. George, Nicholas V. Passalacqua, Darren A. Solomon, David M. Schauble, Brittania J. Bintz, Hannah L Noel
Beginning in 2020, Western Carolina University (WCU)'s Forensic Anthropology Program began a research partnership with the Bureau of Alcohol, Tobacco, Firearms, and Explosives (ATF) Fire Programs and Training Branch on burned human remains via a continuing education (CE) course. This course has evolved since its original inception to include a controlled burn utilizing donated human remains. The content of the CE course is primarily geared toward law enforcement, fire investigators, medicolegal investigators, and crime scene technicians. The 2021 course was the first to utilize a donor from WCU's Body Donation Program, a willed‐body program where donors or their next‐of‐kin can provide additional consent for skeletal modification and/or genetics studies. In addition to the educational purposes of running a controlled burn within a flashover cell, small research projects are also being conducted. Bullets were inserted into the 2021 donor to test various detection methods for utility within fire investigations. For the 2022 course, WCU's Forensic Science Program joined this partnership; they inserted seminal fluid into bodily cavities to examine the recovery of genetic material from a secondary contributor after a high‐intensity, low‐duration fire. The preservation of sharp force trauma in soft tissues was also examined during this course. Genetic material recovery was retested in the 2023 course, as well as examinations of cranial blunt force trauma and blood preservation within a scene. These courses are generating a sample of thermally altered human remains for future research and education, in addition to providing insights useful for fire investigators.This article is categorized under:Forensic Anthropology > Trauma AnalysisForensic Anthropology > Taphonomic Changes and the Environment
从 2020 年开始,西卡罗来纳大学 (WCU) 的法医人类学项目开始与美国烟酒枪械与爆炸物管理局 (ATF) 消防计划与培训处合作,通过继续教育 (CE) 课程对焚烧过的遗骸进行研究。该课程自最初开设以来不断发展,现已包括利用捐赠遗骸进行控制焚烧。CE 课程的内容主要面向执法人员、消防调查员、法医调查员和犯罪现场技术人员。2021 年的课程首次利用了华盛顿大学遗体捐献计划的捐献者,这是一项遗体捐献计划,捐献者或其近亲可以提供额外的同意书,以便进行骨骼改造和/或遗传学研究。除了在闪燃池内进行受控燃烧的教育目的外,还开展了小型研究项目。在 2021 年的捐献者身上插入了子弹,以测试各种探测方法在火灾调查中的实用性。在 2022 年的课程中,西华大学的法医学项目加入了这一合作伙伴关系;他们将精液插入体腔,以检查高强度、低持续时间火灾后从二次供体中恢复遗传物质的情况。该课程还研究了尖锐外伤在软组织中的保存情况。在 2023 年的课程中重新测试了遗传物质的恢复,以及颅骨钝器创伤和现场血液保存的检查。除了为火灾调查人员提供有用的见解外,这些课程还为未来的研究和教育提供了热变化人类遗骸样本。本文归类为:法医人类学 > 创伤分析法医人类学 > 岩石变化与环境
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引用次数: 0
An innovative way to use 3D modeling on burnt one to differentiate heat fractures from blunt and sharp force trauma 在烧伤部位使用三维建模的创新方法,将热骨折与钝器和锐器创伤区分开来
Pub Date : 2024-07-03 DOI: 10.1002/wfs2.1525
Hanna Friedlander, S. Adeeb, Pamela Mayne Correia, Devon Stone, Elizabeth Brooks‐Lim
Assessments of blunt and sharp force trauma in forensic research are frequently reliant on research with individual long bones. As a result, information on the interpretation of the trauma on irregular bones is limited in unburned bones and an even bigger discrepancy is found if the fracture relates to blunt/sharp force in cremated bone. This research strives to differentiate between traumatic fractures and heat fractures in flat and irregular bones. Five human calottes and five human hemipelves were exposed to either blunt or sharp force trauma and then all were incompletely cremated. One hundred and eighty fractures, representing a mixture of traumatic and heat fractures, were captured using a Keyence VHX‐2000 digital microscope and analysis was done in combination with 3D software, Geomagic Studio 2014 and Geomagic Design X (2016). With virtual reconstructions and reverse engineering facilitated by the software, we were able to discern fracture boundaries, slopes, and variances between fracture types. 3D representation provided the ability to differentiate peri‐mortem trauma from heat fractures based on curvature analysis of fracture walls. Evidence of trauma types (blunt versus sharp) were found to be distinguishable at their impact site based on this curvature examination; however, shallow, secondary or tertiary trauma fractures were difficult to discern from heat fractures. Blunt force trauma impact sites and sharp force trauma impact sites were easily identifiable; secondary trauma fractures were sometimes clearly noted but may be misinterpreted. Overall, deep trauma fractures and heat fractures can be discerned from one another using this technology.This article is categorized under:Forensic Anthropology > Taphonomic Changes and the EnvironmentForensic Anthropology > Trauma AnalysisForensic Chemistry and Trace Evidence > Fire Debris Analysis
法医学研究中对钝器和锐器创伤的评估往往依赖于对单个长骨的研究。因此,在未焚烧的骨骼中,对不规则骨骼上创伤的解释信息十分有限,而在火化骨骼中,如果骨折与钝力/锐力有关,则差异会更大。本研究致力于区分扁平骨和不规则骨的创伤骨折和热骨折。研究人员将五块人类卡勒特骨和五块人类半髋骨置于钝力或锐器创伤之下,然后对所有这些骨骼进行不完全火化。使用 KEYENCE VHX-2000 数字显微镜拍摄了 180 处骨折,其中包括创伤骨折和热骨折,并结合 Geomagic Studio 2014 和 Geomagic Design X (2016) 等三维软件进行了分析。通过软件提供的虚拟重建和逆向工程,我们能够辨别断裂边界、坡度和断裂类型之间的差异。根据骨折壁的曲率分析,三维表示法能够区分死前创伤和热骨折。根据这种曲率检查发现,创伤类型(钝伤与锐伤)的证据在其撞击部位是可以区分的;但是,浅层、二级或三级创伤骨折很难与热骨折区分开来。钝力外伤撞击部位和锐力外伤撞击部位很容易辨别;继发性外伤骨折有时会被清楚地注意到,但可能会被误解。总体而言,使用该技术可以辨别深层创伤骨折和热骨折。本文所属分类:法医人类学 > 岩石学变化与环境法医人类学 > 创伤分析法医化学与痕量证据 > 火残分析
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引用次数: 0
Building on the critical skills framework of top crime scene examiners to recruit high‐caliber crime scene investigation candidates 以顶级犯罪现场检查员的关键技能框架为基础,招聘高素质的犯罪现场调查候选人
Pub Date : 2024-05-20 DOI: 10.1002/wfs2.1519
S. Kelty, Phoebe R Joshua, James Robertson
Crime scene investigation is challenging and demands highly skilled crime scene examiners. Mistakes occurring during the processing of a crime scene have been associated with higher risk of ineffective investigations where poor quality, missed, or contaminated forensic samples are relied on by police to inform which suspects to charge and can lead to miscarriages of justice and wrongful imprisonments. Hiring new crime scene examiners with the potential to develop into top performers can result in enhanced crime scene investigation and reduce the risk of errors at crime scenes. A series of papers between 2012 and 2023 identified a cluster of seven critical skills and attributes that set top crime scene examiners apart from their lesser performing peers. These seven skills and attributes clusters are as follows: approach to work, life, and stress management; cognitive abilities; communication and interpersonal skills; holistic knowledge base; life and work experience; and professional demeanor. In this perspective paper, we present strategies for developing a recruitment program to target these skill sets which involve the use of appropriate psychometric testing, collaborative team‐based recruitment, team hiring panels, and crime scene observational assessment tasks at interview stage. We also discuss the costs and logistics associated with psychological testing (in Australian dollars). We finish by exploring the potential challenges of attracting younger people (generation Z and Y) to forensic science roles, including crime scene investigation.This article is categorized under:Crime Scene Investigation > Crime Scene ExaminationCrime Scene Investigation > Crime Scene ReconstructionCrime Scene Investigation > Education and Formation
犯罪现场调查是一项具有挑战性的工作,需要犯罪现场检验人员具备高超的技能。在犯罪现场处理过程中出现的错误与调查无效的风险较高有关,因为警方会依据质量差、遗漏或污染的法医样本来确定对哪些嫌疑人提出指控,这可能会导致误判和错误监禁。聘用新的犯罪现场检验员并将其培养成优秀人才,可以加强犯罪现场调查并降低在犯罪现场出错的风险。2012 年至 2023 年间的一系列论文确定了七项关键技能和属性,这些技能和属性使顶尖的犯罪现场勘验人员有别于表现较差的同行。这七项技能和特质如下:工作、生活和压力管理方法;认知能力;沟通和人际交往技能;综合知识基础;生活和工作经验;以及职业风范。在本文中,我们介绍了针对这些技能组合制定招聘计划的策略,其中包括在面试阶段使用适当的心理测试、基于团队的合作招聘、团队招聘小组和犯罪现场观察评估任务。我们还讨论了与心理测试相关的成本和物流(按澳元计算)。最后,我们探讨了吸引年轻人(Z世代和Y世代)从事包括犯罪现场调查在内的法医学工作可能面临的挑战。本文归类为:犯罪现场调查 > 犯罪现场检查犯罪现场调查 > 犯罪现场重建犯罪现场调查 > 教育和培养
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引用次数: 0
Virtual reality aiding fire scene investigation: A thankless endeavor or catalyst for change? 虚拟现实协助火灾现场调查:吃力不讨好的工作还是变革的催化剂?
Pub Date : 2024-05-01 DOI: 10.1002/wfs2.1518
Vincenzo Rinaldi, E. Ljungkvist, Benny Thomsen, N. NicDaéid
The training of a fire investigator should lay the foundations for a systematic, evidence‐based, and unbiased analysis of a fire scene. The ability of the investigators to determine the origin, cause and development of a fire is gained through education, experience based on on‐the‐job training and focused continuous professional development (CPD). Practitioners have met these requirements for several decades through formal examinations and specialized training. Additionally, several countries provide training in full‐scale (simulated) fire scenes for public and private sector fire investigators. These scenes may also be used for ongoing competency and proficiency testing. However, simulated fire scene setups are costly, limited to a single excavation event, and often short‐lived due to deterioration upon use and ease of contamination. The COVID‐19 pandemic highlighted the need, and provided the opportunity, for developing both virtual/hybrid training and competence testing, prompting the exploration of novel technologies in this area. A cross‐border multi‐agency partnership between academics, forensic practitioners, and forensic scientists explored the feasibility of leveraging digitalization techniques to support immersive virtual reality (VR). We outline the critical aspects and requirements beyond the challenges connected to technical feasibility, which the forensic science and judicial community demand so that technological development would be considered admissible within the justice system.This article is categorized under:Crime Scene Investigation > Crime Scene Documentation and VisualizationDigital and Multimedia Science > Multimedia Forensics
对火灾调查人员的培训应为对火灾现场进行系统、循证和公正的分析奠定基础。调查人员确定火灾起源、原因和发展的能力是通过教育、基于在职培训的经验和有针对性的持续专业发展(CPD)获得的。几十年来,从业人员通过正规考试和专业培训达到了这些要求。此外,一些国家还为公共和私营部门的火灾调查人员提供全面(模拟)火灾现场培训。这些场景也可用于持续的能力和水平测试。不过,模拟火灾现场的设置成本高昂,仅限于一次挖掘活动,而且由于使用后会变质和容易污染,通常寿命较短。COVID-19 大流行突显了开发虚拟/混合培训和能力测试的必要性,并为其提供了机会,从而促使在这一领域探索新技术。学者、法医从业人员和法医科学家之间的跨境多机构合作伙伴关系探索了利用数字化技术来支持沉浸式虚拟现实(VR)的可行性。我们概述了与技术可行性相关的挑战之外的关键方面和要求,这些方面和要求是法医学和司法界所要求的,这样技术开发才会被司法系统认为是可接受的。
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引用次数: 0
Bone color changes and interpretation of the temperature/duration of fire exposure to human remains in the forensic context 骨色变化和法医背景下人体遗骸受火温度/持续时间的解释
Pub Date : 2024-04-11 DOI: 10.1002/wfs2.1517
Alison Galloway, Elayne Pope, Chelsey Juarez
In both the archeological and forensic literature, experimental data have been used to equate the color of burned bone with the temperature to which it was exposed. While this may be applicable in the tightly controlled oxidizing environment of a furnace or crematorium, it is not representative of the more realistic dynamic conditions of fire events involving structures, vehicles, outdoors, and so forth, that are encountered in forensic anthropology casework. In this piece, we review the literature at the cross section of bone color and temperature and compare it to our own observations from experimental burns. We generate a cautionary path forward for future research as well as interpretation in applied contexts. Implying temperature based on burned bone color ignores other significant variables that are related to the degradation of the bone's organic matrix like, the amounts and types of fuels, duration, fluctuations of temperature, oxygen level of the environment, byproducts of combustion, and other variables unique to each scene. Research results on bone color and temperature derived from controlled environments using non‐fleshed bone samples, are not applicable for use in forensic casework involving burned human remains from fleshed bodies. We argue that to do so distracts from other contributing variables and information that can be derived from the analysis of fire patterns on burned human remains and burned bones. Finally, we caution about using bone color alone to estimate temperatures of the fire, which makes incorrect assumptions about fire behavior and could jeopardize court testimony if this method is used as a standard. We raise three issues if a direct correlation between temperature and bone color are used in court: (1) other important factors are ignored, (2) a universal unvaried exposure of the body is assumed, and (3) the expert risks impeachment given conflicting data on the correlation.This article is categorized under:Forensic Medicine > Death Scene InvestigationForensic Anthropology > Taphonomic Changes and the Environment
在考古和法医文献中,实验数据被用来将烧焦骨头的颜色等同于其所处的温度。虽然这可能适用于火炉或火葬场严格控制的氧化环境,但并不能代表法医人类学案例工作中遇到的涉及结构、车辆、户外等更真实的火灾事件动态条件。在这篇文章中,我们回顾了有关骨骼颜色和温度横截面的文献,并将其与我们自己从实验性燃烧中观察到的结果进行了比较。我们为今后的研究以及应用环境中的解释提出了一条谨慎的道路。根据烧焦的骨头颜色推测温度忽略了与骨头有机基质降解有关的其他重要变量,如燃料的数量和类型、持续时间、温度波动、环境中的氧气水平、燃烧的副产品以及每个场景特有的其他变量。在受控环境下使用非肉体骨骼样本得出的骨骼颜色和温度研究结果,不适用于涉及肉体被烧毁的人类遗骸的法医案件工作。我们认为,这样做会分散对其他变量和信息的注意力,而这些变量和信息可以通过分析被焚烧遗骸和被焚烧骨骼上的火烧模式得出。最后,我们提醒大家不要仅仅使用骨头的颜色来估计火灾的温度,这样会对火灾行为做出不正确的假设,如果将这种方法作为标准,可能会危及法庭证词。如果在法庭上使用温度与骨骼颜色之间的直接相关性,我们提出了三个问题:(本文归类于:法医学 > 死亡现场调查法证人类学 > 岩石学变化与环境
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引用次数: 0
Mass spectrometry based proteomics: Changing the impact of protein analysis in forensic science 基于质谱的蛋白质组学:改变蛋白质分析在法医学中的影响
Pub Date : 2024-04-08 DOI: 10.1002/wfs2.1516
Romy Elise Keane, R. Tidy, Glendon J. Parker, J. Gummer, Colin Priddis
Mass spectrometry‐based proteomics, born from the invention of soft ionization, has revolutionized protein analysis. In‐depth characterization of complex and variable mixtures of proteins, such as that found at crime‐scenes, is now possible. This capability continues to expand with advancements in mass spectral instrumentation and computational power, allowing for the development of dynamic data acquisition approaches and sophisticated bioinformatics tools for data interpretation. Proteins are the major component of all biological material, as a result they have been of interest to forensic practitioners for centuries. While DNA is the gold standard for identification, proteins are able to provide contextual information about biological material not available through genomic approaches alone. Mass spectrometry‐based proteomics has expanded the breadth and ease of forensic protein analysis with several emerging applications that promise to become common place as investigative tools, particularly in forensic serology and toxicology. This includes the targeted analysis of protein biomarkers for the classification of biological tissue and fluids, the detection of protein toxins such as ricin and identification of peptide hormones such as insulin. The ability of protein analysis to leverage information from the genome has been demonstrated to achieve individual identification from biological material, an emerging technology that can be used in the absence of extractable DNA. In recent years, there has been considerable scrutiny of forensic techniques that lack objectivity and a statistical basis, an alternative molecular approach for forensic analysis that utilizes the high specificity of mass spectrometry, is a welcome addition with many potential future applications.This article is categorized under:Forensic Chemistry and Trace Evidence > Trace Evidence
基于质谱技术的蛋白质组学诞生于软离子化技术的发明,为蛋白质分析带来了革命性的变化。现在,对复杂多变的蛋白质混合物(如犯罪现场发现的蛋白质混合物)进行深入表征已成为可能。随着质谱仪器和计算能力的进步,这种能力还在不断扩大,从而可以开发动态数据采集方法和用于数据解读的先进生物信息学工具。蛋白质是所有生物材料的主要成分,因此,几个世纪以来,法医从业人员一直对蛋白质很感兴趣。DNA 是鉴定的黄金标准,而蛋白质则能提供生物材料的背景信息,这是基因组学方法所无法提供的。以质谱为基础的蛋白质组学扩展了法医蛋白质分析的广度和便利性,一些新兴的应用有望成为常用的调查工具,特别是在法医血清学和毒理学领域。这包括有针对性地分析用于生物组织和体液分类的蛋白质生物标记物、检测蛋白质毒素(如蓖麻毒素)和鉴定肽类激素(如胰岛素)。蛋白质分析利用基因组信息的能力已得到证实,可从生物材料中实现个体识别,这是一项新兴技术,可在没有可提取 DNA 的情况下使用。近年来,对缺乏客观性和统计基础的法医技术进行了大量的审查,利用质谱的高特异性进行法医分析的另一种分子方法是一种受欢迎的新方法,具有许多潜在的未来应用。
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WIREs Forensic Science
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