Pub Date : 2024-08-08DOI: 10.31989/ffhd.v14i8.1388
Camille Lelong, Antoine Mandoux, Nicolas Houyoux, Sylvie Defrere, P. Mariage
Background: Cognitive function declines with aging, primarily due to reduced neuronal plasticity, decreased release of trophic factors, and neuronal stress. Genetic and environmental factors have been shown to impact cognitive function. Diets enriched with neuroactive ingredients have been proposed to support cognitive functions in humans. Several neuroactive compounds, including ginsenosides, have been identified in Panax ginseng. Many studies show that the roots of this plant promote neuroprotection and neuroplasticity. Objective: The aim of this study was to examine the impact of the red Panax ginseng roots cultivated in an innovative vertical farming technology. The effects of the red Panax ginseng roots were investigated to determine whether they could (1) support neuronal plasticity and neuronal survival during stress in vitro and (2) improve cognitive function (short-term memory) in vivo. Methods: An extract of the Botalys red Panax ginseng root powder was tested in vitro on primary hippocampal and cortical neurons, injured or not, with glutamate. Its effects on the neuritogenesis and the synaptogenesis was investigated as well as its neuroprotective efficacy. Moreover, the effect of the red Panax ginseng root powder was investigated in vivo on the cognitive functions of aged animals. Results: The results demonstrated that prolonged treatment of hippocampal neurons with the red Panax ginseng extract significantly prompts synapse formation and neurite elongation in primary cultures of hippocampal neurons. In addition, the red Panax ginseng extract protected neurons from glutamate-induced excitotoxicity in a primary culture of cortical neurons. In aging mice, oral administration of red Panax ginseng root powder for 7 days significantly improved short-term memory deficit associated with aging. Conclusion: Altogether, these results indicate that red Panax ginseng root promotes neuronal plasticity and synaptogenesis in in vitro models and improves short-term memory deficits in in vivo models of aging. Keywords: Panax ginseng, neuronal plasticity, cognitive functions, in vitro, in vivo
{"title":"Red Panax ginseng root promotes neuronal plasticity in vitro and improves cognitive function in aged animals","authors":"Camille Lelong, Antoine Mandoux, Nicolas Houyoux, Sylvie Defrere, P. Mariage","doi":"10.31989/ffhd.v14i8.1388","DOIUrl":"https://doi.org/10.31989/ffhd.v14i8.1388","url":null,"abstract":"Background: Cognitive function declines with aging, primarily due to reduced neuronal plasticity, decreased release of trophic factors, and neuronal stress. Genetic and environmental factors have been shown to impact cognitive function. Diets enriched with neuroactive ingredients have been proposed to support cognitive functions in humans. Several neuroactive compounds, including ginsenosides, have been identified in Panax ginseng. Many studies show that the roots of this plant promote neuroprotection and neuroplasticity.\u0000Objective: The aim of this study was to examine the impact of the red Panax ginseng roots cultivated in an innovative vertical farming technology. The effects of the red Panax ginseng roots were investigated to determine whether they could (1) support neuronal plasticity and neuronal survival during stress in vitro and (2) improve cognitive function (short-term memory) in vivo. \u0000Methods: An extract of the Botalys red Panax ginseng root powder was tested in vitro on primary hippocampal and cortical neurons, injured or not, with glutamate. Its effects on the neuritogenesis and the synaptogenesis was investigated as well as its neuroprotective efficacy. Moreover, the effect of the red Panax ginseng root powder was investigated in vivo on the cognitive functions of aged animals.\u0000Results: The results demonstrated that prolonged treatment of hippocampal neurons with the red Panax ginseng extract significantly prompts synapse formation and neurite elongation in primary cultures of hippocampal neurons. In addition, the red Panax ginseng extract protected neurons from glutamate-induced excitotoxicity in a primary culture of cortical neurons. In aging mice, oral administration of red Panax ginseng root powder for 7 days significantly improved short-term memory deficit associated with aging.\u0000Conclusion: Altogether, these results indicate that red Panax ginseng root promotes neuronal plasticity and synaptogenesis in in vitro models and improves short-term memory deficits in in vivo models of aging. \u0000Keywords: Panax ginseng, neuronal plasticity, cognitive functions, in vitro, in vivo","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"30 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141925982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-12DOI: 10.31989/ffhd.v14i6.1352
Mohammed Talib Abed, Nuhad Mohammed Kassim, Lina Nidhal Sajjad
Background: Babies and young children require adequate and high-quality nutrition to grow up healthy and reach their full potential. Breast milk is a baby's healthiest option because it contains all the essential nutrients they need, and it has long been known that breastfeeding is good for both mom and baby. Objectives: To determine the relationship between mothers' feeding practices and child weight status under two years old. Materials and Methods: A descriptive correlational study design was carried out at Babylon Teaching Hospital for maternity and children and Al Nour Hospital, which was applied from December 2019 to the end of February 2020 as a period for data collection. The sample consisted of (150) mothers admitted with their infants in pediatric wards. The instrument was adopted and finally constructed after an extensive literature review to be ready for data collection and consumed 15-20 minutes with each participant. Results: The study indicated that (76.7%) of mothers have bad feeding practices regarding child feeding, whereas (23.3%) have good feeding practices. In addition to other significant results, our study demonstrates a considerable positive relationship between the mode of feeding and a child's weight. Conclusions: The vast majority of mothers have bad child-feeding practices. There was a significant correlation between the type of feeding and infant weight and a strong association between the mother’s feeding practice and child weight. Keywords: Weight, Feeding practice, Two Years Old Children, Effects, Relation.
{"title":"Weight in relation to feeding practice among two years old children in Al-Hillah City","authors":"Mohammed Talib Abed, Nuhad Mohammed Kassim, Lina Nidhal Sajjad","doi":"10.31989/ffhd.v14i6.1352","DOIUrl":"https://doi.org/10.31989/ffhd.v14i6.1352","url":null,"abstract":"Background: Babies and young children require adequate and high-quality nutrition to grow up healthy and reach their full potential. Breast milk is a baby's healthiest option because it contains all the essential nutrients they need, and it has long been known that breastfeeding is good for both mom and baby.\u0000Objectives: To determine the relationship between mothers' feeding practices and child weight status under two years old.\u0000Materials and Methods: A descriptive correlational study design was carried out at Babylon Teaching Hospital for maternity and children and Al Nour Hospital, which was applied from December 2019 to the end of February 2020 as a period for data collection. The sample consisted of (150) mothers admitted with their infants in pediatric wards. The instrument was adopted and finally constructed after an extensive literature review to be ready for data collection and consumed 15-20 minutes with each participant.\u0000Results: The study indicated that (76.7%) of mothers have bad feeding practices regarding child feeding, whereas (23.3%) have good feeding practices. In addition to other significant results, our study demonstrates a considerable positive relationship between the mode of feeding and a child's weight.\u0000Conclusions: The vast majority of mothers have bad child-feeding practices. There was a significant correlation between the type of feeding and infant weight and a strong association between the mother’s feeding practice and child weight.\u0000Keywords: Weight, Feeding practice, Two Years Old Children, Effects, Relation.","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"52 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141353045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Curcumin has several health benefits due to its potent antioxidant and anti-inflammatory properties, but its bioavailability is very low, limiting its potential. We have developed a novel curcumin formulation, TS-P1, which has an 85.2-fold higher bioavailability of curcumin than raw curcumin. Objective: To investigate the efficacy of TS-P1 on fatigue, mood status, and autonomic function. Methods: Ninety healthy Japanese adults were randomized to the placebo or TS-P1 group and took either the placebo or TS-P1 containing 150 mg of curcumin for 12 weeks. Visual Analogue Scale for Fatigue (VAS-F) scores, Profile of Mood States Questionnaire second edition (POMS2) scores, and autonomic functions were measured every 4 weeks. Results: Both groups showed an improvement in the VAS-F over the study period, but there was no significant difference between the placebo and TS-P1 groups. However, the mean change from baseline in the anger and depression scores of the POMS2, particularly in those aged 40 and over, in the TS-P1 group showed a greater range of decline at week 12. The mean change from baseline in LnLF and LF/HF also decreased in the TS-P1 group. Blood pressure also decreased in the TS-P1 group. Conclusions: These data suggest that TS-P1 induced a parasympathetic dominant state, and that curcumin has an important role in the regulation of autonomic balance. 12 weeks of supplementation of TS-P1 is expected to improve both mood status and vascular function. Trial registration: UMIN000050377 Foundation: Theravalues Corporation Keywords: curcumin; TS-P1: Theracurmin Super; mood status; autonomic balance, parasympathetic dominance; vascular function
{"title":"Improvement in autonomic balance through 12-week supplementation of a novel curcumin formulation in healthy Japanese adults: A randomized, placebo-controlled study","authors":"Kuwabara Yoshitaka, Hyunjin Lee, Akiko Hirose, Yuji Makino, Kyohei Hashimoto, Misaki Sakata, Tadashi Watanabe","doi":"10.31989/ffhd.v14i6.1330","DOIUrl":"https://doi.org/10.31989/ffhd.v14i6.1330","url":null,"abstract":"Background: Curcumin has several health benefits due to its potent antioxidant and anti-inflammatory properties,\u0000but its bioavailability is very low, limiting its potential. We have developed a novel curcumin formulation, TS-P1, which\u0000has an 85.2-fold higher bioavailability of curcumin than raw curcumin.\u0000 \u0000Objective: To investigate the efficacy of TS-P1 on fatigue, mood status, and autonomic function.\u0000 \u0000Methods: Ninety healthy Japanese adults were randomized to the placebo or TS-P1 group and took either the placebo\u0000or TS-P1 containing 150 mg of curcumin for 12 weeks. Visual Analogue Scale for Fatigue (VAS-F) scores, Profile of\u0000Mood States Questionnaire second edition (POMS2) scores, and autonomic functions were measured every 4 weeks.\u0000 \u0000Results: Both groups showed an improvement in the VAS-F over the study period, but there was no significant\u0000difference between the placebo and TS-P1 groups. However, the mean change from baseline in the anger and\u0000depression scores of the POMS2, particularly in those aged 40 and over, in the TS-P1 group showed a greater range\u0000of decline at week 12. The mean change from baseline in LnLF and LF/HF also decreased in the TS-P1 group. Blood\u0000pressure also decreased in the TS-P1 group.\u0000 \u0000Conclusions: These data suggest that TS-P1 induced a parasympathetic dominant state, and that curcumin has an\u0000important role in the regulation of autonomic balance. 12 weeks of supplementation of TS-P1 is expected to improve\u0000both mood status and vascular function.\u0000 \u0000Trial registration: UMIN000050377\u0000 \u0000Foundation: Theravalues Corporation\u0000 \u0000Keywords: curcumin; TS-P1: Theracurmin Super; mood status; autonomic balance, parasympathetic dominance;\u0000vascular function","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141368976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-03DOI: 10.31989/ffhd.v14i6.1342
Yushi Hashizume, M. Tandia
Background: Atherosclerotic cardiovascular disease (ASCVD) continues to be a significant contributor to global mortality, impacting over 523 million individuals worldwide. Dyslipidemia stands as one of the foremost risk factors for ASCVD. Thus, prioritizing the reduction of low-density lipoprotein cholesterol (LDL-C) levels is essential in mitigating cardiovascular complications. Objective: This study aimed to evaluate the lipid-lowering activity of a dietary supplement containing monoglucosyl rutin (MR) in individuals with low to mild hypercholesterolemia. Methods: This was a randomized, placebo-controlled, double-blind, parallel-group study conducted from April 20 to December 24, 2022. The study population included 56 healthy Japanese adult participants with LDL-C levels between 120–139 mg/dL who were randomly allocated to either the MR or placebo groups (n = 28/group) using a computerized random number generator. 200 mg of MR or placebo divided into 3 tablets was given daily; participants were instructed to take 1 tablet with water after each meal for 12 weeks. The main focus was on measuring the serum LDL-C level as the primary outcome, with additional attention given to secondary outcomes such as serum high-density lipoprotein cholesterol (HDL-C), total cholesterol, and nonHDL-C levels. The study also evaluated the percentage of participants achieving serum LDL-C levels below 120 mg/dL after the 12-week intervention. Assessments were conducted after 4, 8, and 12 weeks of intervention. Results: There were 54 (27 in each group) participants in the per-protocol set (PPS) and 53 (placebo group, 26; MR group, 27) participants in the modified PPS (mPPS). A statistically significant group difference in serum LDL-C levels was observed (P < 0.05), with a 5.0% decrease from preintervention to after 12 weeks. The reduction in LDL-C levels coincided with (statistically significant?) decreases in total cholesterol levels, nonHDL-C levels, and the LDL-C/HDL-C ratio. During the study period, there was no adverse event or concern about the safety of MR. Conclusions: MR has potential as a preventive or therapeutic tool for improving improve long-term health and reducing cardiovascular morbidity. Keywords: monoglucosyl rutin, flavonoid glycoside, low-density lipoprotein cholesterol, hyperlipidemia, lipid profile Trial registration number: UMIN000047790
{"title":"Monoglucosyl rutin, a flavonoid glycoside, improves low-density lipoprotein-cholesterol levels in healthy adults: A randomized controlled trial","authors":"Yushi Hashizume, M. Tandia","doi":"10.31989/ffhd.v14i6.1342","DOIUrl":"https://doi.org/10.31989/ffhd.v14i6.1342","url":null,"abstract":"Background: Atherosclerotic cardiovascular disease (ASCVD) continues to be a significant contributor to global mortality, impacting over 523 million individuals worldwide. Dyslipidemia stands as one of the foremost risk factors for ASCVD. Thus, prioritizing the reduction of low-density lipoprotein cholesterol (LDL-C) levels is essential in mitigating cardiovascular complications. \u0000Objective: This study aimed to evaluate the lipid-lowering activity of a dietary supplement containing monoglucosyl rutin (MR) in individuals with low to mild hypercholesterolemia.\u0000Methods: This was a randomized, placebo-controlled, double-blind, parallel-group study conducted from April 20 to December 24, 2022. The study population included 56 healthy Japanese adult participants with LDL-C levels between 120–139 mg/dL who were randomly allocated to either the MR or placebo groups (n = 28/group) using a computerized random number generator. 200 mg of MR or placebo divided into 3 tablets was given daily; participants were instructed to take 1 tablet with water after each meal for 12 weeks. The main focus was on measuring the serum LDL-C level as the primary outcome, with additional attention given to secondary outcomes such as serum high-density lipoprotein cholesterol (HDL-C), total cholesterol, and nonHDL-C levels. The study also evaluated the percentage of participants achieving serum LDL-C levels below 120 mg/dL after the 12-week intervention. Assessments were conducted after 4, 8, and 12 weeks of intervention.\u0000Results: There were 54 (27 in each group) participants in the per-protocol set (PPS) and 53 (placebo group, 26; MR group, 27) participants in the modified PPS (mPPS). A statistically significant group difference in serum LDL-C levels was observed (P < 0.05), with a 5.0% decrease from preintervention to after 12 weeks. The reduction in LDL-C levels coincided with (statistically significant?) decreases in total cholesterol levels, nonHDL-C levels, and the LDL-C/HDL-C ratio. During the study period, there was no adverse event or concern about the safety of MR.\u0000Conclusions: MR has potential as a preventive or therapeutic tool for improving improve long-term health and reducing cardiovascular morbidity.\u0000Keywords: monoglucosyl rutin, flavonoid glycoside, low-density lipoprotein cholesterol, hyperlipidemia, lipid profile\u0000Trial registration number: UMIN000047790","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"23 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141270864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-03DOI: 10.31989/ffhd.v14i6.1304
Linh Jaafar Sultan, Wafaa Ghazi Fadhil, Mohammed Majid Hamid, Sara Thamer Hadi
Background: Medicinal plants that possess antimicrobial and antioxidant properties have garnered significant attention for their role in maintaining food quality, improving safety, and impeding spoilage. They also can aid in controlling food contamination risks and augmenting the nutritional value of foods. Objective: The study aimed to obtain botanical extracts possessing antimicrobial capabilities and use them to inhibit the growth of molds and yeasts. Additionally, these extracts are aimed at prolonging product shelf life by harnessing their antioxidant attributes. Methods: Several microorganisms, including E. coli and Pseudomonas, were subjected to testing. Ethanolic alcohol, chloroform, and essential oil extracts were prepared; the tablets were then immersed in these solutions to test their efficacy. Results: The leaf extracts displayed significant antimicrobial efficacy against essential oil extracts with Staphylococcus aureus and Serratia marcescens, while the organic extracts, which included ethyl alcohol and chloroform, did not show any inhibitory effects against bacteria. Conclusion: The basil leaf extract demonstrated significant antimicrobial activity against microorganisms, particularly in the case of essential oil extracts compared with other methods of extraction. However, Gram-positive bacteria showed a better response for the essential oils method than that of Gram-negative bacteria. Keywords: Ocimum basilicum; basil leaf; Clevenger; chemical active compounds; essential oil; organic extracts
{"title":"A comparative study of the effect of extracts extracted from Ocimum basilicum leaves using organic extract and essential oil","authors":"Linh Jaafar Sultan, Wafaa Ghazi Fadhil, Mohammed Majid Hamid, Sara Thamer Hadi","doi":"10.31989/ffhd.v14i6.1304","DOIUrl":"https://doi.org/10.31989/ffhd.v14i6.1304","url":null,"abstract":"Background: Medicinal plants that possess antimicrobial and antioxidant properties have garnered significant attention for their role in maintaining food quality, improving safety, and impeding spoilage. They also can aid in controlling food contamination risks and augmenting the nutritional value of foods.\u0000Objective: The study aimed to obtain botanical extracts possessing antimicrobial capabilities and use them to inhibit the growth of molds and yeasts. Additionally, these extracts are aimed at prolonging product shelf life by harnessing their antioxidant attributes.\u0000Methods: Several microorganisms, including E. coli and Pseudomonas, were subjected to testing. Ethanolic alcohol, chloroform, and essential oil extracts were prepared; the tablets were then immersed in these solutions to test their efficacy.\u0000Results: The leaf extracts displayed significant antimicrobial efficacy against essential oil extracts with Staphylococcus aureus and Serratia marcescens, while the organic extracts, which included ethyl alcohol and chloroform, did not show any inhibitory effects against bacteria.\u0000Conclusion: The basil leaf extract demonstrated significant antimicrobial activity against microorganisms, particularly in the case of essential oil extracts compared with other methods of extraction. However, Gram-positive bacteria showed a better response for the essential oils method than that of Gram-negative bacteria. \u0000Keywords: Ocimum basilicum; basil leaf; Clevenger; chemical active compounds; essential oil; organic extracts","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"56 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141268871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Lacticaseibacillus paracasei 327 (L. paracasei 327) is a lactic acid bacteria isolated from brown rice. It has been reported that sterilized L. paracasei 327 improves bowel movement. Given the reported relationship between intestinal microbiota and bowel movement, we hypothesized that L. paracasei 327 also improves the intestinal environment through its involvement in fluctuations in the composition of the intestinal microbiota. Objective: The purpose of this study was to verify the effects of the consumption of food containing sterilized L. paracasei 327 on the intestinal environment in healthy Japanese adults. Methods: A randomized, double-blind, placebo-controlled, parallel-group trial was conducted in 110 healthy adults aged 20 to 64 years with a defecation frequency of three to five times per week. Participants were divided into two groups, one receiving 25 mg (approximately 5x1010 bacteria) of sterile L. paracasei 327 once daily for two weeks (55 participants) and other receiving placebo for two weeks (55 participants), to test its effect on the intestinal environment. Results: Intestinal microbiota analysis showed that L. paracasei 327 ingestion resulted in a trend towards an increase in Bacteroides and a significant decrease in Clostridium cluster IV in each taxonomic group compared to the placebo group. In addition, for each operational taxonomic units (OTUs), Bacteroides_OTU_469 and Clostridium subcluster XIVa_OTU_754 were significantly increased, and Clostridium cluster IX, Akkermansia_OTU_110 tended to increase, while Clostridium cluster IV_OTU_749 significantly decreased. Furthermore, defecation days and defecation frequency increased significantly. Conclusion: The presumptive species of bacteria in the taxonomic group and OTUs increased by L. paracasei 327 ingestion included short-chain fatty acid-producing bacteria. In addition, Clostridium cluster IV_OTU_749, which was reduced by L. paracasei 327 ingestion, may also be involved in the production of putrefactive products in the intestine. Therefore, these findings suggest that L. paracasei 327 ingestion improves the intestinal environment by increasing short-chain fatty acid-producing bacteria, suppressing putrefactive-producing bacteria, and improving defecation. Trial registration: UMIN000049859 (UMIN-CTR)Keywords: Lactic acid bacteria; Postbiotics; Lacticaseibacillus paracasei 327; Intestinal microbiota; Defecation; Clinical trial
{"title":"Effects of Lacticaseibacillus paracasei 327 intake on the intestinal environment in healthy adult Japanese: a randomized, double-blind, placebo-controlled, parallel-group trial","authors":"Yoshie Senda-Sugimoto, Toshihiro Mihara, Yuki Higuchi, Kimiko Uchiyama, Tsuyoshi Takara, Hajime Takahashi","doi":"10.31989/ffhd.v14i3.1336","DOIUrl":"https://doi.org/10.31989/ffhd.v14i3.1336","url":null,"abstract":"Background: Lacticaseibacillus paracasei 327 (L. paracasei 327) is a lactic acid bacteria isolated from brown rice. It has been reported that sterilized L. paracasei 327 improves bowel movement. Given the reported relationship between intestinal microbiota and bowel movement, we hypothesized that L. paracasei 327 also improves the intestinal environment through its involvement in fluctuations in the composition of the intestinal microbiota.\u0000Objective: The purpose of this study was to verify the effects of the consumption of food containing sterilized L. paracasei 327 on the intestinal environment in healthy Japanese adults.\u0000Methods: A randomized, double-blind, placebo-controlled, parallel-group trial was conducted in 110 healthy adults aged 20 to 64 years with a defecation frequency of three to five times per week. Participants were divided into two groups, one receiving 25 mg (approximately 5x1010 bacteria) of sterile L. paracasei 327 once daily for two weeks (55 participants) and other receiving placebo for two weeks (55 participants), to test its effect on the intestinal environment.\u0000Results: Intestinal microbiota analysis showed that L. paracasei 327 ingestion resulted in a trend towards an increase in Bacteroides and a significant decrease in Clostridium cluster IV in each taxonomic group compared to the placebo group. In addition, for each operational taxonomic units (OTUs), Bacteroides_OTU_469 and Clostridium subcluster XIVa_OTU_754 were significantly increased, and Clostridium cluster IX, Akkermansia_OTU_110 tended to increase, while Clostridium cluster IV_OTU_749 significantly decreased. Furthermore, defecation days and defecation frequency increased significantly.\u0000Conclusion: The presumptive species of bacteria in the taxonomic group and OTUs increased by L. paracasei 327 ingestion included short-chain fatty acid-producing bacteria. In addition, Clostridium cluster IV_OTU_749, which was reduced by L. paracasei 327 ingestion, may also be involved in the production of putrefactive products in the intestine. Therefore, these findings suggest that L. paracasei 327 ingestion improves the intestinal environment by increasing short-chain fatty acid-producing bacteria, suppressing putrefactive-producing bacteria, and improving defecation.\u0000Trial registration: UMIN000049859 (UMIN-CTR)Keywords: Lactic acid bacteria; Postbiotics; Lacticaseibacillus paracasei 327; Intestinal microbiota; Defecation; Clinical trial","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"70 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140229949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-13DOI: 10.31989/ffhd.v14i3.1331
Ryuji Takeda, Yuji Kuriyama, Yasushi Yoshida
Background: Recently, bean ferritin has been attracting considerable attention as a new source of iron, that is available to vegetarians. Although high rates of iron absorption and bioavailability from this protein have been reported, clinical data on its efficacy remain scarce. Objective: In this study, we administered bean ferritin iron to premenopausal Japanese women for nine weeks, starting immediately after menstruation, to evaluate their recovery from low hemoglobin levels as one sign of anemia. Methods: Participants in the test supplement group received an iron intake of 5 mg from one capsule containing bean extract (SloIron®) for five weeks, which was increased to 10 mg (i.e., two capsules) from the 6th to the 9th week. The study evaluated the change in hemoglobin levels as the primary endpoint, and hematocrit, red blood cell count, serum iron, MCH (mean corpuscular hemoglobin), MCHC (mean corpuscular hemoglobin concentration), serum ferritin, TSAT (TIBC), serum zinc, serum copper, anemia symptoms questionnaire, OSA sleep inventory, and the anti-fatigue questionnaire as the secondary endpoints. Results: Our results showed a significant difference (P=0.03) in the change in hemoglobin levels between the groups after nine weeks of intake, confirming the restorative effect of bean ferritin on low hemoglobin levels caused by menstruation. Moreover, a significant difference (P=0.01) was observed in the amount of change in MCHC between the two groups after five weeks of intake, and after nine weeks of intake, a significant difference in the change in both MCH (P=0.02) and MCHC (P<0.01) was observed between the groups. A significant difference (P=0.03) was observed in the change in serum ferritin levels after nine weeks of intake. Conclusion: The study confirmed that iron supplementation from bean ferritin is an effective treatment for low hemoglobin and low ferritin levels caused by menstruation. Keywords: Ferritin, Bean ferritin, Menstruation, Serum iron, Hemoglobin
{"title":"Restorative effect of bean ferritin iron on low hemoglobin level in premenopausal women with menstruation-induced anemia: A randomized, double-blind placebo-controlled intergroup trial","authors":"Ryuji Takeda, Yuji Kuriyama, Yasushi Yoshida","doi":"10.31989/ffhd.v14i3.1331","DOIUrl":"https://doi.org/10.31989/ffhd.v14i3.1331","url":null,"abstract":"Background: Recently, bean ferritin has been attracting considerable attention as a new source of iron, that is available to vegetarians. Although high rates of iron absorption and bioavailability from this protein have been reported, clinical data on its efficacy remain scarce.\u0000Objective: In this study, we administered bean ferritin iron to premenopausal Japanese women for nine weeks, starting immediately after menstruation, to evaluate their recovery from low hemoglobin levels as one sign of anemia.\u0000Methods: Participants in the test supplement group received an iron intake of 5 mg from one capsule containing bean extract (SloIron®) for five weeks, which was increased to 10 mg (i.e., two capsules) from the 6th to the 9th week. The study evaluated the change in hemoglobin levels as the primary endpoint, and hematocrit, red blood cell count, serum iron, MCH (mean corpuscular hemoglobin), MCHC (mean corpuscular hemoglobin concentration), serum ferritin, TSAT (TIBC), serum zinc, serum copper, anemia symptoms questionnaire, OSA sleep inventory, and the anti-fatigue questionnaire as the secondary endpoints.\u0000Results: Our results showed a significant difference (P=0.03) in the change in hemoglobin levels between the groups after nine weeks of intake, confirming the restorative effect of bean ferritin on low hemoglobin levels caused by menstruation. Moreover, a significant difference (P=0.01) was observed in the amount of change in MCHC between the two groups after five weeks of intake, and after nine weeks of intake, a significant difference in the change in both MCH (P=0.02) and MCHC (P<0.01) was observed between the groups. A significant difference (P=0.03) was observed in the change in serum ferritin levels after nine weeks of intake.\u0000Conclusion: The study confirmed that iron supplementation from bean ferritin is an effective treatment for low hemoglobin and low ferritin levels caused by menstruation.\u0000Keywords: Ferritin, Bean ferritin, Menstruation, Serum iron, Hemoglobin","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"14 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140247896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-04DOI: 10.31989/ffhd.v14i3.1325
M. Moriwaki, Aya Abe, Mahendra P. Kapoor, Akiko Yamaguchi, Saki Okamoto, Makoto Ozeki
Background: Hesperidin, a flavonoid glycoside, is widely found in the peels and rinds of citrus fruits, offering various physiological benefits. However, its effectiveness is hindered by challenges related to insolubility and low bioavailability. To overcome these obstacles, we developed the Hesperetin-7-glucoside -β-cyclodextrin inclusion complex (HCD). This complex demonstrates superior solubility and bioavailability compared to hesperidin. In a previous study where participants consumed 300 mg/day of HCD for 12 weeks, there was a notable improvement in endothelial dysfunction. Importantly, no significant adverse clinical events were reported during this period. Objective: To evaluate the safety of the excessive intake of HCD in Healthy Japanese subjects. Methods: Fourteen healthy male and female volunteers (with a mean age of 39.1±9.1) participated in this excessive HCD intake clinical trial. Subjects took 1500 mg/day HCD, which was five times the dosage of 300 mg/day HCD, for 4 consecutive weeks. Physical examination, blood tests, and uric tests were performed during this period. Results: Results demonstrated no significant differences at 2, and 4 weeks compared to the baseline at 0 weeks with 1500 mg HCD (equivalent to 195 mg HPTG) supplementation in healthy subjects. Conclusions: It has been demonstrated that there are no safety concerns when consuming 1500 mg of HCD daily, continuously for 4 weeks. Keywords: Safety, Bioavailability, Clinical trials, Hesperidin, Food, Foods with Function Claims, Overdose supplementation, Cyclodextrin Trial registration: UMIN-CTR (Trial ID: UMIN000051960) Foundation: Taiyo Kagaku Co. Ltd.
{"title":"Safety evaluation of excessive intake of Hesperetin-7-Glucoside-β-Cyclodextrin Inclusion Complex in Healthy Japanese Subjects","authors":"M. Moriwaki, Aya Abe, Mahendra P. Kapoor, Akiko Yamaguchi, Saki Okamoto, Makoto Ozeki","doi":"10.31989/ffhd.v14i3.1325","DOIUrl":"https://doi.org/10.31989/ffhd.v14i3.1325","url":null,"abstract":"Background: Hesperidin, a flavonoid glycoside, is widely found in the peels and rinds of citrus fruits, offering various physiological benefits. However, its effectiveness is hindered by challenges related to insolubility and low bioavailability. To overcome these obstacles, we developed the Hesperetin-7-glucoside -β-cyclodextrin inclusion complex (HCD). This complex demonstrates superior solubility and bioavailability compared to hesperidin. In a previous study where participants consumed 300 mg/day of HCD for 12 weeks, there was a notable improvement in endothelial dysfunction. Importantly, no significant adverse clinical events were reported during this period.\u0000Objective: To evaluate the safety of the excessive intake of HCD in Healthy Japanese subjects.\u0000Methods: Fourteen healthy male and female volunteers (with a mean age of 39.1±9.1) participated in this excessive HCD intake clinical trial. Subjects took 1500 mg/day HCD, which was five times the dosage of 300 mg/day HCD, for 4 consecutive weeks. Physical examination, blood tests, and uric tests were performed during this period.\u0000Results: Results demonstrated no significant differences at 2, and 4 weeks compared to the baseline at 0 weeks with 1500 mg HCD (equivalent to 195 mg HPTG) supplementation in healthy subjects.\u0000Conclusions: It has been demonstrated that there are no safety concerns when consuming 1500 mg of HCD daily, continuously for 4 weeks.\u0000Keywords: Safety, Bioavailability, Clinical trials, Hesperidin, Food, Foods with Function Claims, Overdose supplementation, Cyclodextrin\u0000Trial registration: UMIN-CTR (Trial ID: UMIN000051960)\u0000Foundation: Taiyo Kagaku Co. Ltd.","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"75 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140080068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.31989/ffhd.v14i2.1317
Gayane Melyan, Gagik Santrosyan
Background: The cultivation of stone fruits is of primary importance in Armenia. Their fruits contain antioxidants, fiber, potassium, vitamin A, C, E, minerals, etc., which have a beneficial effect on human health and prevent many diseases. The concentration of those components varies depending on ecological factors, cultivar, rootstock, cultural practices, etc. Clonal rootstocks are important for increasing orchard density, tree uniformity, and high yields, and they can also affect fruit quality. In vitro culture is a valuable method for rapid propagation of high-quality, virus free plant material. Objective: The purpose of this study was to develop in vitro production technology for the stone-fruit rootstock cultivar Evrica 99, and to determine if rootstocks affect some fresh fruit traits of ´Yerevani´ and ´Sateni´ apricot cultivars. Methods: The shoot apical meristem and lateral bud served as explants for shoot regeneration. Different sterilizing agents at various periods of exposure were used for the explant surface sterilization. Various concentrations of phytohormones, both individually and in combinations, were employed for in vitro regeneration and rooting of plants. The titratable acidity (TA), dry matter (DM), vitamin C, mineral content, total carotenoids (TC), and sugar contents were evaluated in fresh fruit. Results: The most optimal option for explant surface sterilization was the gradual application of calcium hypochlorite [Ca (ClO)2] (2.0% solution, exposure time 10 min) and ethanol (70% solution, exposure time 20 s), as a result of which we had 75.5% survival rate of explants. The efficient medium for in vitro shoot regeneration was MS supplemented with 6-Benzylaminopurine (BAP) 0.8 mg/l, Kinetin (Kin) 0.2 mg/l, and Gibberellic acid (GA3) 1.0 mg/l. The half-strength Murashige and Skoog (MS) medium containing 0.8 mg/l indole-3-butyric acid (IBA) was optimal for in vitro rooting. Rooted plants were successfully adapted with a survival rate of 85.0%. The defined method can be successfully used for 'Evrica 99' cultivar micropropagation. The results obtained showed that fruit quality strongly depended on both the varieties and the rootstock tested. Conclusion: In the current study, an alternative in vitro propagation technology for rootstock cultivar ´ Evrica 99 was developed by direct organogenesis, enabling mass-scale production of virus-free plants that is suitable for commercial purposes as well. The apricot cultivars ´Yerevani´ and ´Sateni´ grafted on the virus-free rootstock cultivar Evrica 99 showed higher fruit quality traits, which are essential for human health and diet. Keywords: ´Evrica 99, fucnctional foods, in vitro regeneration, micropropagation, plant growth regulators, stone fruit rootstock, tissue culture.
{"title":"In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit","authors":"Gayane Melyan, Gagik Santrosyan","doi":"10.31989/ffhd.v14i2.1317","DOIUrl":"https://doi.org/10.31989/ffhd.v14i2.1317","url":null,"abstract":"Background: The cultivation of stone fruits is of primary importance in Armenia. Their fruits contain antioxidants, fiber, potassium, vitamin A, C, E, minerals, etc., which have a beneficial effect on human health and prevent many diseases. The concentration of those components varies depending on ecological factors, cultivar, rootstock, cultural practices, etc. Clonal rootstocks are important for increasing orchard density, tree uniformity, and high yields, and they can also affect fruit quality. In vitro culture is a valuable method for rapid propagation of high-quality, virus free plant material.\u0000Objective: The purpose of this study was to develop in vitro production technology for the stone-fruit rootstock cultivar Evrica 99, and to determine if rootstocks affect some fresh fruit traits of ´Yerevani´ and ´Sateni´ apricot cultivars.\u0000Methods: The shoot apical meristem and lateral bud served as explants for shoot regeneration. Different sterilizing agents at various periods of exposure were used for the explant surface sterilization. Various concentrations of phytohormones, both individually and in combinations, were employed for in vitro regeneration and rooting of plants. The titratable acidity (TA), dry matter (DM), vitamin C, mineral content, total carotenoids (TC), and sugar contents were evaluated in fresh fruit.\u0000Results: The most optimal option for explant surface sterilization was the gradual application of calcium hypochlorite [Ca (ClO)2] (2.0% solution, exposure time 10 min) and ethanol (70% solution, exposure time 20 s), as a result of which we had 75.5% survival rate of explants. The efficient medium for in vitro shoot regeneration was MS supplemented with 6-Benzylaminopurine (BAP) 0.8 mg/l, Kinetin (Kin) 0.2 mg/l, and Gibberellic acid (GA3) 1.0 mg/l. The half-strength Murashige and Skoog (MS) medium containing 0.8 mg/l indole-3-butyric acid (IBA) was optimal for in vitro rooting. Rooted plants were successfully adapted with a survival rate of 85.0%. The defined method can be successfully used for 'Evrica 99' cultivar micropropagation. The results obtained showed that fruit quality strongly depended on both the varieties and the rootstock tested.\u0000Conclusion: In the current study, an alternative in vitro propagation technology for rootstock cultivar ´ Evrica 99 was developed by direct organogenesis, enabling mass-scale production of virus-free plants that is suitable for commercial purposes as well. The apricot cultivars ´Yerevani´ and ´Sateni´ grafted on the virus-free rootstock cultivar Evrica 99 showed higher fruit quality traits, which are essential for human health and diet.\u0000Keywords: ´Evrica 99, fucnctional foods, in vitro regeneration, micropropagation, plant growth regulators, stone fruit rootstock, tissue culture.","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"268 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-15DOI: 10.31989/ffhd.v14i2.1317
Gayane Melyan, Gagik Santrosyan
Background: The cultivation of stone fruits is of primary importance in Armenia. Their fruits contain antioxidants, fiber, potassium, vitamin A, C, E, minerals, etc., which have a beneficial effect on human health and prevent many diseases. The concentration of those components varies depending on ecological factors, cultivar, rootstock, cultural practices, etc. Clonal rootstocks are important for increasing orchard density, tree uniformity, and high yields, and they can also affect fruit quality. In vitro culture is a valuable method for rapid propagation of high-quality, virus free plant material. Objective: The purpose of this study was to develop in vitro production technology for the stone-fruit rootstock cultivar Evrica 99, and to determine if rootstocks affect some fresh fruit traits of ´Yerevani´ and ´Sateni´ apricot cultivars. Methods: The shoot apical meristem and lateral bud served as explants for shoot regeneration. Different sterilizing agents at various periods of exposure were used for the explant surface sterilization. Various concentrations of phytohormones, both individually and in combinations, were employed for in vitro regeneration and rooting of plants. The titratable acidity (TA), dry matter (DM), vitamin C, mineral content, total carotenoids (TC), and sugar contents were evaluated in fresh fruit. Results: The most optimal option for explant surface sterilization was the gradual application of calcium hypochlorite [Ca (ClO)2] (2.0% solution, exposure time 10 min) and ethanol (70% solution, exposure time 20 s), as a result of which we had 75.5% survival rate of explants. The efficient medium for in vitro shoot regeneration was MS supplemented with 6-Benzylaminopurine (BAP) 0.8 mg/l, Kinetin (Kin) 0.2 mg/l, and Gibberellic acid (GA3) 1.0 mg/l. The half-strength Murashige and Skoog (MS) medium containing 0.8 mg/l indole-3-butyric acid (IBA) was optimal for in vitro rooting. Rooted plants were successfully adapted with a survival rate of 85.0%. The defined method can be successfully used for 'Evrica 99' cultivar micropropagation. The results obtained showed that fruit quality strongly depended on both the varieties and the rootstock tested. Conclusion: In the current study, an alternative in vitro propagation technology for rootstock cultivar ´ Evrica 99 was developed by direct organogenesis, enabling mass-scale production of virus-free plants that is suitable for commercial purposes as well. The apricot cultivars ´Yerevani´ and ´Sateni´ grafted on the virus-free rootstock cultivar Evrica 99 showed higher fruit quality traits, which are essential for human health and diet. Keywords: ´Evrica 99, fucnctional foods, in vitro regeneration, micropropagation, plant growth regulators, stone fruit rootstock, tissue culture.
{"title":"In vitro propagation of stone fruit rootstock cultivar Evrica 99 and its influence on some phytochemical traits of fresh apricot fruit","authors":"Gayane Melyan, Gagik Santrosyan","doi":"10.31989/ffhd.v14i2.1317","DOIUrl":"https://doi.org/10.31989/ffhd.v14i2.1317","url":null,"abstract":"Background: The cultivation of stone fruits is of primary importance in Armenia. Their fruits contain antioxidants, fiber, potassium, vitamin A, C, E, minerals, etc., which have a beneficial effect on human health and prevent many diseases. The concentration of those components varies depending on ecological factors, cultivar, rootstock, cultural practices, etc. Clonal rootstocks are important for increasing orchard density, tree uniformity, and high yields, and they can also affect fruit quality. In vitro culture is a valuable method for rapid propagation of high-quality, virus free plant material.\u0000Objective: The purpose of this study was to develop in vitro production technology for the stone-fruit rootstock cultivar Evrica 99, and to determine if rootstocks affect some fresh fruit traits of ´Yerevani´ and ´Sateni´ apricot cultivars.\u0000Methods: The shoot apical meristem and lateral bud served as explants for shoot regeneration. Different sterilizing agents at various periods of exposure were used for the explant surface sterilization. Various concentrations of phytohormones, both individually and in combinations, were employed for in vitro regeneration and rooting of plants. The titratable acidity (TA), dry matter (DM), vitamin C, mineral content, total carotenoids (TC), and sugar contents were evaluated in fresh fruit.\u0000Results: The most optimal option for explant surface sterilization was the gradual application of calcium hypochlorite [Ca (ClO)2] (2.0% solution, exposure time 10 min) and ethanol (70% solution, exposure time 20 s), as a result of which we had 75.5% survival rate of explants. The efficient medium for in vitro shoot regeneration was MS supplemented with 6-Benzylaminopurine (BAP) 0.8 mg/l, Kinetin (Kin) 0.2 mg/l, and Gibberellic acid (GA3) 1.0 mg/l. The half-strength Murashige and Skoog (MS) medium containing 0.8 mg/l indole-3-butyric acid (IBA) was optimal for in vitro rooting. Rooted plants were successfully adapted with a survival rate of 85.0%. The defined method can be successfully used for 'Evrica 99' cultivar micropropagation. The results obtained showed that fruit quality strongly depended on both the varieties and the rootstock tested.\u0000Conclusion: In the current study, an alternative in vitro propagation technology for rootstock cultivar ´ Evrica 99 was developed by direct organogenesis, enabling mass-scale production of virus-free plants that is suitable for commercial purposes as well. The apricot cultivars ´Yerevani´ and ´Sateni´ grafted on the virus-free rootstock cultivar Evrica 99 showed higher fruit quality traits, which are essential for human health and diet.\u0000Keywords: ´Evrica 99, fucnctional foods, in vitro regeneration, micropropagation, plant growth regulators, stone fruit rootstock, tissue culture.","PeriodicalId":507841,"journal":{"name":"Functional Foods in Health and Disease","volume":"65 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139775211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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