Pub Date : 2024-04-02DOI: 10.33925/1683-3759-2024-895
S. B. Ulitovskiy, L. Orekhova, O. Kalinina, A. A. Leontev, E. Loboda
Relevance. The oral health of the global population is fundamentally influenced by the incidence and severity of dental caries and periodontal diseases. To combat these issues, developed nations devise and execute preventive programs, tailoring their efforts to address the DEF (Decayed, Extracted, and Filled teeth) index across various age demographics. This approach signifies a paradigm shift, with governments now prioritizing the support of oral health through the introduction of comprehensive preventive strategies aimed at combating prevalent oral diseases. The efficacy of these programs, however, hinges on the occurrence of dental caries and, by extension, the availability of oral health professionals. This availability, in turn, shapes the landscape of health facilities—whether public or private—determining their ability to cater to community needs for oral health services. Consequently, the capacity to implement preventive measures is gauged against these dynamics. Furthermore, the nature and scope of preventive actions within these programs vary from one country to another, influenced primarily by the allocation of healthcare budgets by respective governments.Materials and methods. Analysis of scientific articles and original research from the PubMed, ResearchGate, ScienceDirect for the last 10 years. The search was carried out by keywords: dental prevention programs in the world, prevention of dental health, organization of preventive care in the world. An examination of research articles and original papers was conducted, drawing from databases such as PubMed, ResearchGate, and ScienceDirect over the last decade. The search employed specific key phrases, including "dental prevention programs worldwide", "dental health prevention", and "global organization of preventive dental care".Results. The initial advancement towards the creation and implementation of fluoride prophylaxis programs occurred following the discovery of fluoride's presence in water. A pivotal moment occurred in 1949 at Indiana State University when J.C. Muhler introduced the idea of fluoride toothpaste (specifically sodium fluoride) as a means to combat dental caries in children. Subsequent innovations included the use of silver fluoride for argentation. European countries took a holistic approach, emphasizing oral hygiene, fluoride toothpaste usage, and balanced nutrition. Meanwhile, the US and EU shifted towards a preventive approach within health insurance frameworks. A critical factor influencing the effectiveness of these preventive care programs is the availability of oral health professionals per 10,000 people in the population, alongside social workers advocating for healthy living and oral health. The engagement of more oral health professionals in preventive efforts correlates with a reduction in the incidence and severity of common oral diseases.Conclusion. The six stages of preventive care development and transformation highlight the evolving trends in globa
{"title":"The world's experience of introducing prophylactic programs in dentistry","authors":"S. B. Ulitovskiy, L. Orekhova, O. Kalinina, A. A. Leontev, E. Loboda","doi":"10.33925/1683-3759-2024-895","DOIUrl":"https://doi.org/10.33925/1683-3759-2024-895","url":null,"abstract":"Relevance. The oral health of the global population is fundamentally influenced by the incidence and severity of dental caries and periodontal diseases. To combat these issues, developed nations devise and execute preventive programs, tailoring their efforts to address the DEF (Decayed, Extracted, and Filled teeth) index across various age demographics. This approach signifies a paradigm shift, with governments now prioritizing the support of oral health through the introduction of comprehensive preventive strategies aimed at combating prevalent oral diseases. The efficacy of these programs, however, hinges on the occurrence of dental caries and, by extension, the availability of oral health professionals. This availability, in turn, shapes the landscape of health facilities—whether public or private—determining their ability to cater to community needs for oral health services. Consequently, the capacity to implement preventive measures is gauged against these dynamics. Furthermore, the nature and scope of preventive actions within these programs vary from one country to another, influenced primarily by the allocation of healthcare budgets by respective governments.Materials and methods. Analysis of scientific articles and original research from the PubMed, ResearchGate, ScienceDirect for the last 10 years. The search was carried out by keywords: dental prevention programs in the world, prevention of dental health, organization of preventive care in the world. An examination of research articles and original papers was conducted, drawing from databases such as PubMed, ResearchGate, and ScienceDirect over the last decade. The search employed specific key phrases, including \"dental prevention programs worldwide\", \"dental health prevention\", and \"global organization of preventive dental care\".Results. The initial advancement towards the creation and implementation of fluoride prophylaxis programs occurred following the discovery of fluoride's presence in water. A pivotal moment occurred in 1949 at Indiana State University when J.C. Muhler introduced the idea of fluoride toothpaste (specifically sodium fluoride) as a means to combat dental caries in children. Subsequent innovations included the use of silver fluoride for argentation. European countries took a holistic approach, emphasizing oral hygiene, fluoride toothpaste usage, and balanced nutrition. Meanwhile, the US and EU shifted towards a preventive approach within health insurance frameworks. A critical factor influencing the effectiveness of these preventive care programs is the availability of oral health professionals per 10,000 people in the population, alongside social workers advocating for healthy living and oral health. The engagement of more oral health professionals in preventive efforts correlates with a reduction in the incidence and severity of common oral diseases.Conclusion. The six stages of preventive care development and transformation highlight the evolving trends in globa","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140753889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-27DOI: 10.33925/1683-3759-2024-864
M. A. Lisina, I. I. Borodulina, V. Chirsky, L. V. Vasilyeva
Relevance. Periodontal diseases are among the most prevalent conditions affecting the oral cavity, often leading to tooth loss. Comorbid conditions, especially type 2 diabetes mellitus (DM2), can significantly influence the health of periodontal tissues by altering microcirculatory dynamics.Materials and methods. This study aimed to evaluate periodontal tissue health in patients with chronic periodontitis, both with and without DM2 comorbidity. Periodontal microcirculation was examined using the Laser Doppler Flowmetry (LDF) technique, specifically with a LAKK-M device (Russia). A total of 106 patients were recruited and categorized into four groups based on their DM2 status and the severity of chronic periodontitis.Results. The study revealed group-specific differences in periodontal microcirculation parameters, analyzed through nonlinear dynamics. Notably, patients with chronic periodontitis and comorbid DM2 exhibited the most significant alterations. A phase portrait analysis further delineated the microcirculation health disparities between groups.Conclusion. There were marked differences in microcirculation health among chronic periodontitis patients with and without DM2 comorbidity, as evidenced by significant changes in the nonlinear dynamics-based phase portraits.
{"title":"Comparative analysis of LDF phase portraits in periodontal microcirculation among patient with type 2 diabetes mellitus","authors":"M. A. Lisina, I. I. Borodulina, V. Chirsky, L. V. Vasilyeva","doi":"10.33925/1683-3759-2024-864","DOIUrl":"https://doi.org/10.33925/1683-3759-2024-864","url":null,"abstract":"Relevance. Periodontal diseases are among the most prevalent conditions affecting the oral cavity, often leading to tooth loss. Comorbid conditions, especially type 2 diabetes mellitus (DM2), can significantly influence the health of periodontal tissues by altering microcirculatory dynamics.Materials and methods. This study aimed to evaluate periodontal tissue health in patients with chronic periodontitis, both with and without DM2 comorbidity. Periodontal microcirculation was examined using the Laser Doppler Flowmetry (LDF) technique, specifically with a LAKK-M device (Russia). A total of 106 patients were recruited and categorized into four groups based on their DM2 status and the severity of chronic periodontitis.Results. The study revealed group-specific differences in periodontal microcirculation parameters, analyzed through nonlinear dynamics. Notably, patients with chronic periodontitis and comorbid DM2 exhibited the most significant alterations. A phase portrait analysis further delineated the microcirculation health disparities between groups.Conclusion. There were marked differences in microcirculation health among chronic periodontitis patients with and without DM2 comorbidity, as evidenced by significant changes in the nonlinear dynamics-based phase portraits.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140376479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-26DOI: 10.33925/1683-3759-2024-865
R. A. Sadikov, Yuliya V. Plotkina, E. М. Chernovol, A. L. Rubezov, E. Mikhailova, А. R. Sadikov
Relevance. The transgingival part of the prosthetic components of implant systems is a substrate for the primary adaptation of soft tissues and the formation of the gingiva collar - a complex of tissues acting as a biological barrier and ensuring the normal functioning of artificial supports in the microenvironment of the oral cavity. At the same time, the morphology of the surface of the abutment which will be in constant contact with the gingiva part during the loading period is of great importance. A change in the properties of the surface morphology of the transgingival part of orthopedic structures is fraught with the formation of a microbial bio-film in the subgingival zone with the with the subsequent development of mucositis and peri-implantitis. Based on the above, the study of the surface characteristics of orthopedic components of implantation systems seems relevant in the light of understanding the pathogenesis of inflammatory processes and assessing risk factors for the development of complications of dental implantation.Purpose. Study of differences in the elemental composition and features (or characteristics) of the surface morphology of orthopedic components - transgingival implant abutments from a number of modern manufacturers.Materials and methods. Prosthetic components from five manufacturing companies were selected for the study. For the assessment, elemental analysis of the surface of the components was used using a Hitachi S-3400N scanning electron microscope with an Oxford Instruments X-Max20 energy dispersive spectrometer, as well as morphological analysis using a TESCAN VEGA 3 scanning electron microscope. The samples were assessed for surface uniformity, the presence of inclusions, cracks and marks.Results. The study revealed significant differences in the components both in elemental composition and surface topography. The results obtained can be summarized in the form of a table.Conclusion. The method of comprehensive surface assessment used in the study can be recommended for describing and comparing orthopedic components from different manufacturing companies.
{"title":"Comprehensive analysis of the elemental composition and surface relief of titanium abutments from contemporary manufactures","authors":"R. A. Sadikov, Yuliya V. Plotkina, E. М. Chernovol, A. L. Rubezov, E. Mikhailova, А. R. Sadikov","doi":"10.33925/1683-3759-2024-865","DOIUrl":"https://doi.org/10.33925/1683-3759-2024-865","url":null,"abstract":"Relevance. The transgingival part of the prosthetic components of implant systems is a substrate for the primary adaptation of soft tissues and the formation of the gingiva collar - a complex of tissues acting as a biological barrier and ensuring the normal functioning of artificial supports in the microenvironment of the oral cavity. At the same time, the morphology of the surface of the abutment which will be in constant contact with the gingiva part during the loading period is of great importance. A change in the properties of the surface morphology of the transgingival part of orthopedic structures is fraught with the formation of a microbial bio-film in the subgingival zone with the with the subsequent development of mucositis and peri-implantitis. Based on the above, the study of the surface characteristics of orthopedic components of implantation systems seems relevant in the light of understanding the pathogenesis of inflammatory processes and assessing risk factors for the development of complications of dental implantation.Purpose. Study of differences in the elemental composition and features (or characteristics) of the surface morphology of orthopedic components - transgingival implant abutments from a number of modern manufacturers.Materials and methods. Prosthetic components from five manufacturing companies were selected for the study. For the assessment, elemental analysis of the surface of the components was used using a Hitachi S-3400N scanning electron microscope with an Oxford Instruments X-Max20 energy dispersive spectrometer, as well as morphological analysis using a TESCAN VEGA 3 scanning electron microscope. The samples were assessed for surface uniformity, the presence of inclusions, cracks and marks.Results. The study revealed significant differences in the components both in elemental composition and surface topography. The results obtained can be summarized in the form of a table.Conclusion. The method of comprehensive surface assessment used in the study can be recommended for describing and comparing orthopedic components from different manufacturing companies.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140379469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.33925/1683-3759-2024-887
A. M. Samoukina, V. Chervinets
Relevance. The oral microbiota, including its interactions with viruses, plays a crucial role in an individual's immune system. Investigating these microbiota-virus associations as biomarkers for personalized health assessments through advanced laboratory testing is an emerging research field.Materials and methods. This study conducted an extensive clinical laboratory examination of the oral microbiota-virus associations across various biotopes of the digestive tract in 417 participants across different health and age categories: namely, generally healthy children and adolescents in health groups I and II (n = 253, with 456 microbiota-virus associations from 127 individuals); health group III (n = 229, with 436 microbiota-virus associations from 225 individuals); and preterm infants classified by degree of low birth weight (n = 271, with 398 microbiota-virus associations from 55 individuals).Results. Our findings showed a decrease in indigenous microbiota by 9.7% and an increase in potentially pathogenic microbiota by 19.8% across age groups (p ≤ 0.05). There was also a 5.8-fold increase in the viral components, notably Epstein-Barr virus and HHV-6, in the oral cavities of generally healthy children in health groups I and II. In health group III children, there was a notable increase in opportunistic pathogens and the detection of cytomegalovirus DNA (38.1%, p ≤ 0.05). Furthermore, the predominance of Staphylococcus species in the opportunistic pathogenic microbiota, which correlates with low birth weight, was identified as a prognostic indicator of poor microecological conditions and decreased immune resistance in preterm infants undergoing prolonged hospital care, with detection rates of 19% in extremely low birth weight and 4% in low birth weight groups (p ≤ 0.05).Conclusion. Evaluating immune resistance in children of various health statuses and age groups through the analysis of oral microbiota-virus associations is advisable for outpatient healthcare settings. This assessment can guide the provision of comprehensive physical exams for children and adolescents and the formulation of personalized treatment plans based on identified risk groups.
相关性。口腔微生物群，包括其与病毒的相互作用，在个人免疫系统中发挥着至关重要的作用。通过先进的实验室检测将这些微生物群与病毒的关联作为个性化健康评估的生物标志物进行研究是一个新兴的研究领域。本研究对 417 名不同健康状况和年龄段的参与者进行了广泛的口腔微生物群-病毒关联临床实验室检查，这些参与者包括：健康组 I 和健康组 II 中一般健康的儿童和青少年（n = 253，127 人中有 456 个微生物群-病毒关联）；健康组 III（n = 229，225 人中有 436 个微生物群-病毒关联）；以及按出生体重过轻程度分类的早产儿（n = 271，55 人中有 398 个微生物群-病毒关联）。我们的研究结果表明，不同年龄组的本地微生物群减少了 9.7%，潜在致病微生物群增加了 19.8%（p ≤ 0.05）。健康组 I 和健康组 II 一般健康儿童口腔中的病毒成分，特别是 Epstein-Barr 病毒和 HHV-6 也增加了 5.8 倍。在健康组 III 的儿童中，机会性病原体和巨细胞病毒 DNA 的检出率明显增加（38.1%，p ≤ 0.05）。此外，葡萄球菌在机会致病微生物群中占主导地位，这与低出生体重有关，被认为是接受长期住院治疗的早产儿微生态条件差和免疫抵抗力下降的预后指标，在极低出生体重组和低出生体重组中的检出率分别为19%和4%（P≤0.05）。通过分析口腔微生物群与病毒的关系来评估不同健康状况和年龄组儿童的免疫抵抗力，是门诊医疗机构的明智之举。这种评估可以指导为儿童和青少年提供全面体检，并根据已确定的风险群体制定个性化治疗方案。
{"title":"Monitoring oral microbiota-virus associations as biomarkers of immune resistance","authors":"A. M. Samoukina, V. Chervinets","doi":"10.33925/1683-3759-2024-887","DOIUrl":"https://doi.org/10.33925/1683-3759-2024-887","url":null,"abstract":"Relevance. The oral microbiota, including its interactions with viruses, plays a crucial role in an individual's immune system. Investigating these microbiota-virus associations as biomarkers for personalized health assessments through advanced laboratory testing is an emerging research field.Materials and methods. This study conducted an extensive clinical laboratory examination of the oral microbiota-virus associations across various biotopes of the digestive tract in 417 participants across different health and age categories: namely, generally healthy children and adolescents in health groups I and II (n = 253, with 456 microbiota-virus associations from 127 individuals); health group III (n = 229, with 436 microbiota-virus associations from 225 individuals); and preterm infants classified by degree of low birth weight (n = 271, with 398 microbiota-virus associations from 55 individuals).Results. Our findings showed a decrease in indigenous microbiota by 9.7% and an increase in potentially pathogenic microbiota by 19.8% across age groups (p ≤ 0.05). There was also a 5.8-fold increase in the viral components, notably Epstein-Barr virus and HHV-6, in the oral cavities of generally healthy children in health groups I and II. In health group III children, there was a notable increase in opportunistic pathogens and the detection of cytomegalovirus DNA (38.1%, p ≤ 0.05). Furthermore, the predominance of Staphylococcus species in the opportunistic pathogenic microbiota, which correlates with low birth weight, was identified as a prognostic indicator of poor microecological conditions and decreased immune resistance in preterm infants undergoing prolonged hospital care, with detection rates of 19% in extremely low birth weight and 4% in low birth weight groups (p ≤ 0.05).Conclusion. Evaluating immune resistance in children of various health statuses and age groups through the analysis of oral microbiota-virus associations is advisable for outpatient healthcare settings. This assessment can guide the provision of comprehensive physical exams for children and adolescents and the formulation of personalized treatment plans based on identified risk groups.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-26DOI: 10.33925/1683-3759-2024-821
A. S. Galieva, N. V. Davidovich, A. S. Oрravin, E. N. Bashilova, K. R. Ryumin, T. A. Bazhukova
Relevance. Periodontal inflammations result from chronic, persistent infections triggered by dysbiosis-induced shifts within the microbial community. The high prevalence, multifactorial nature and challenging management of periodontal disease create an environment ripe for ongoing advancements in treatment modalities.Study objective: To validate, using clinical and laboratory test data, the efficacy of combined treatment involving bacteriophages and probiotics in managing chronic periodontitis.Materials and methods. A total of 100 patients aged 18 to 45 with periodontal inflammation underwent clinical laboratory examinations before receiving combination treatment. The patients were divided into two groups: Group 1 received conventional treatment based on clinical recommendations, while Group 2 received sequential treatment with a bacteriophage cocktail (targeting 81 phages against A. actinomycetemcomitans, B. licheniformis, B. fragilis, E. cloacae, E. faecalis, K. pneumonia, S. aureus, S. pyogenes, Wolinella spp. among others) followed by a probiotic containing S. Salivarius. Periodontal pocket lavages served as study material. Periodontal pathogens were isolated using real-time PCR, and proinflammatory cytokines were identified via enzyme immunoassay. Statistical analysis was conducted using STATA v.12 software.Results. Following bacteriophage/probiotic combination treatment, positive changes in study indices and a reduction in periodontopathogenic microbiota were observed. Significant decreases were noted in the levels of key periodontal pathogens: P. gingivalis (5.2-fold decrease, p < 0.001), P. intermedia (3.6-fold decrease, p < 0.001; T. forsythia (3.8-fold decrease, p = 0.905), T. denticola 4-fold decrease, p < 0.001. Levels of IL1-β decreased by factors of 4.29 (p < 0.001) and 8.59 (p = 0.02) in severity subgroups, while IL6 levels decreased by factors of 4.39 (p < 0.001) and 5.94 (p = 0.0002). The lowest TNF-α levels (2.13 in mild CGP subgroup and 2.11 in moderate CGP subgroup) were observed post-treatment (p < 0.001; p = 0.0005).Conclusion. The combination treatment involving the selected drugs exhibited notable therapeutic effectiveness in the management of chronic periodontitis. This approach not only avoided the necessity for systemic antibacterial agents but also facilitated prolonged remission by eradicating periodontopathogenic microbiota in patients with chronic periodontitis. The etiopathogenetic nature of this treatment represents a promising paradigm in the management of periodontal inflammation.
{"title":"The efficacy of bacteriophage/probiotic combination therapy in periodontal treatment","authors":"A. S. Galieva, N. V. Davidovich, A. S. Oрravin, E. N. Bashilova, K. R. Ryumin, T. A. Bazhukova","doi":"10.33925/1683-3759-2024-821","DOIUrl":"https://doi.org/10.33925/1683-3759-2024-821","url":null,"abstract":"Relevance. Periodontal inflammations result from chronic, persistent infections triggered by dysbiosis-induced shifts within the microbial community. The high prevalence, multifactorial nature and challenging management of periodontal disease create an environment ripe for ongoing advancements in treatment modalities.Study objective: To validate, using clinical and laboratory test data, the efficacy of combined treatment involving bacteriophages and probiotics in managing chronic periodontitis.Materials and methods. A total of 100 patients aged 18 to 45 with periodontal inflammation underwent clinical laboratory examinations before receiving combination treatment. The patients were divided into two groups: Group 1 received conventional treatment based on clinical recommendations, while Group 2 received sequential treatment with a bacteriophage cocktail (targeting 81 phages against A. actinomycetemcomitans, B. licheniformis, B. fragilis, E. cloacae, E. faecalis, K. pneumonia, S. aureus, S. pyogenes, Wolinella spp. among others) followed by a probiotic containing S. Salivarius. Periodontal pocket lavages served as study material. Periodontal pathogens were isolated using real-time PCR, and proinflammatory cytokines were identified via enzyme immunoassay. Statistical analysis was conducted using STATA v.12 software.Results. Following bacteriophage/probiotic combination treatment, positive changes in study indices and a reduction in periodontopathogenic microbiota were observed. Significant decreases were noted in the levels of key periodontal pathogens: P. gingivalis (5.2-fold decrease, p < 0.001), P. intermedia (3.6-fold decrease, p < 0.001; T. forsythia (3.8-fold decrease, p = 0.905), T. denticola 4-fold decrease, p < 0.001. Levels of IL1-β decreased by factors of 4.29 (p < 0.001) and 8.59 (p = 0.02) in severity subgroups, while IL6 levels decreased by factors of 4.39 (p < 0.001) and 5.94 (p = 0.0002). The lowest TNF-α levels (2.13 in mild CGP subgroup and 2.11 in moderate CGP subgroup) were observed post-treatment (p < 0.001; p = 0.0005).Conclusion. The combination treatment involving the selected drugs exhibited notable therapeutic effectiveness in the management of chronic periodontitis. This approach not only avoided the necessity for systemic antibacterial agents but also facilitated prolonged remission by eradicating periodontopathogenic microbiota in patients with chronic periodontitis. The etiopathogenetic nature of this treatment represents a promising paradigm in the management of periodontal inflammation.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140430755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-05DOI: 10.33925/1683-3759-2023-843
E. A. Durnovo, E. Galkina, N. Y. Orlinskaya, M. S. Kvashnina, A. Y. Sopina
Relevance. The complex, multi-stage surgical procedure aimed for replacing missing teeth often results in a deficiency of attached keratinized gingiva (AKG) in the intervened area. Despite numerous methods available for soft tissue volume and esthetics reconstruction, only a few demonstrate considerable long-term stability post-surgery. The quest to address this intricate issue has led to the combined utilization of autografts and xenografts Consequently, there is a need to evaluate the tissue structure obtained through this synergy via histological and immunohistochemical examination of the oral mucosa in the reconstructed region.Materials and methods. This study involved analyzing 87 biopsies of oral mucosa obtained from 57 patients aged 28 to 59 years who had undergone previous soft tissue volume and esthetics reconstruction in the AKG zone. Histological and immunohistochemical examinations were conducted on these biopsies, collected six weeks post soft tissue operation. A section of oral mucosa from the hard palate served as the reference sample.Results. The epithelial structure in the examined groups exhibited a well-defined basal layer with a continuous structure comprising two layers of cells without notable atypia, indicating a high cell proliferation capability. Immunohistochemical analysis targeting protein molecules revealed an absence of inflammation. The expression results of the Ki-67 antigen suggested the preservation of cell proliferation capacity and, consequently, the maintenance of tissue regenerative potential at a high level during the remote postoperative period, up to six weeks.Conclusion. This research showcases the efficacy of employing a combination of autografts and xenografts, a conclusion drawn from comprehensive histological and immunohistochemical analyses. Consequently, this approach presents a promising and innovative alternative to conventional methods for soft tissue volume and esthetics reconstruction.
{"title":"Histological and immunohistochemical analysis of autografts and xenografts synergy in rectifying attached keratinized gingiva deficiency","authors":"E. A. Durnovo, E. Galkina, N. Y. Orlinskaya, M. S. Kvashnina, A. Y. Sopina","doi":"10.33925/1683-3759-2023-843","DOIUrl":"https://doi.org/10.33925/1683-3759-2023-843","url":null,"abstract":"Relevance. The complex, multi-stage surgical procedure aimed for replacing missing teeth often results in a deficiency of attached keratinized gingiva (AKG) in the intervened area. Despite numerous methods available for soft tissue volume and esthetics reconstruction, only a few demonstrate considerable long-term stability post-surgery. The quest to address this intricate issue has led to the combined utilization of autografts and xenografts Consequently, there is a need to evaluate the tissue structure obtained through this synergy via histological and immunohistochemical examination of the oral mucosa in the reconstructed region.Materials and methods. This study involved analyzing 87 biopsies of oral mucosa obtained from 57 patients aged 28 to 59 years who had undergone previous soft tissue volume and esthetics reconstruction in the AKG zone. Histological and immunohistochemical examinations were conducted on these biopsies, collected six weeks post soft tissue operation. A section of oral mucosa from the hard palate served as the reference sample.Results. The epithelial structure in the examined groups exhibited a well-defined basal layer with a continuous structure comprising two layers of cells without notable atypia, indicating a high cell proliferation capability. Immunohistochemical analysis targeting protein molecules revealed an absence of inflammation. The expression results of the Ki-67 antigen suggested the preservation of cell proliferation capacity and, consequently, the maintenance of tissue regenerative potential at a high level during the remote postoperative period, up to six weeks.Conclusion. This research showcases the efficacy of employing a combination of autografts and xenografts, a conclusion drawn from comprehensive histological and immunohistochemical analyses. Consequently, this approach presents a promising and innovative alternative to conventional methods for soft tissue volume and esthetics reconstruction.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139864201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-29DOI: 10.33925/1683-3759-2024-859
A. Sheregov, Z. F. Kharaeva, M. S. Mustafaev, D. S. Bozieva, L. R. Asanova
Relevance. The presence of an orthodontic appliance within the oral cavity enhances the formation of supplementary retention sites for the adhesion of microorganisms. The porosity of acrylic base materials, coupled with their limited polishability, further predisposes the surface to contamination by a biofilm of microorganisms. Evaluating the capacity of materials to attract bacterial adherence to their surfaces represents a crucial phase in patient treatment.Purpose. This study aims to perform a comprehensive clinical and laboratory investigation into the adhesive properties of orthodontic plate appliances crafted from various dental plastics concerning the mixed oral microbiota.Materials and methods. In vitro research was conducted, focusing on both removable and non-removable orthodontic plate appliances featuring a base composed of dental plastics (referred to as Sample 1 and Sample 2). The study included 80 orthodontic patients, comprising 50 children aged 8 to 12 years with removable appliances and 30 children aged 8 to 10 years with non-removable plate appliances. Patients were categorized into two groups based on the material of their appliances: the first group, pertaining to those with removable appliances (Subgroup 1A – Sample 1, 25 individuals, and Subgroup 1B – Sample 2, 25 individuals); the second group, comprising individuals with non-removable appliances (Subgroup 2A – Sample 1, 15 individuals, and Subgroup 2B – Sample 2, 15 individuals).Results. In the in vitro investigations, it was observed that the average colonization of removable plates was 100 times lower (4.5 log CFU/ml) compared to non-removable plates (5.5 log CFU/ml; p < 0.05). The average colonization of plates composed of plastic (Sample 2) demonstrated a significant reduction, approximately 200 times (4.0 log CFU/ml), compared to plates made of plastic (Sample 1) (6.0 log CFU/ml; p < 0.05). Upon evaluating the overall colonization of the oral cavity, bacterial growth ranged between 106-7 CFU/ml in patients with appliances made of plastic Sample 1, whereas in patients with appliances made of plastic Sample 2, the range was limited to 103-4 CFU/ml (p < 0,05).Conclusion. The statistical analysis reveals a significant disparity in the adhesive activity of microorganisms to orthodontic plate appliances constructed from materials of distinct production origins, with notably lower adherence observed on smoother surfaces (specifically those made of plastic Sample 2). This underscores the importance of meticulous consideration for the polishability properties inherent in the materials utilized in the manufacturing of orthodontic appliances.
{"title":"Adhesive interaction of orthodontic plate appliances with mixed oral microbiota","authors":"A. Sheregov, Z. F. Kharaeva, M. S. Mustafaev, D. S. Bozieva, L. R. Asanova","doi":"10.33925/1683-3759-2024-859","DOIUrl":"https://doi.org/10.33925/1683-3759-2024-859","url":null,"abstract":"Relevance. The presence of an orthodontic appliance within the oral cavity enhances the formation of supplementary retention sites for the adhesion of microorganisms. The porosity of acrylic base materials, coupled with their limited polishability, further predisposes the surface to contamination by a biofilm of microorganisms. Evaluating the capacity of materials to attract bacterial adherence to their surfaces represents a crucial phase in patient treatment.Purpose. This study aims to perform a comprehensive clinical and laboratory investigation into the adhesive properties of orthodontic plate appliances crafted from various dental plastics concerning the mixed oral microbiota.Materials and methods. In vitro research was conducted, focusing on both removable and non-removable orthodontic plate appliances featuring a base composed of dental plastics (referred to as Sample 1 and Sample 2). The study included 80 orthodontic patients, comprising 50 children aged 8 to 12 years with removable appliances and 30 children aged 8 to 10 years with non-removable plate appliances. Patients were categorized into two groups based on the material of their appliances: the first group, pertaining to those with removable appliances (Subgroup 1A – Sample 1, 25 individuals, and Subgroup 1B – Sample 2, 25 individuals); the second group, comprising individuals with non-removable appliances (Subgroup 2A – Sample 1, 15 individuals, and Subgroup 2B – Sample 2, 15 individuals).Results. In the in vitro investigations, it was observed that the average colonization of removable plates was 100 times lower (4.5 log CFU/ml) compared to non-removable plates (5.5 log CFU/ml; p < 0.05). The average colonization of plates composed of plastic (Sample 2) demonstrated a significant reduction, approximately 200 times (4.0 log CFU/ml), compared to plates made of plastic (Sample 1) (6.0 log CFU/ml; p < 0.05). Upon evaluating the overall colonization of the oral cavity, bacterial growth ranged between 106-7 CFU/ml in patients with appliances made of plastic Sample 1, whereas in patients with appliances made of plastic Sample 2, the range was limited to 103-4 CFU/ml (p < 0,05).Conclusion. The statistical analysis reveals a significant disparity in the adhesive activity of microorganisms to orthodontic plate appliances constructed from materials of distinct production origins, with notably lower adherence observed on smoother surfaces (specifically those made of plastic Sample 2). This underscores the importance of meticulous consideration for the polishability properties inherent in the materials utilized in the manufacturing of orthodontic appliances.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140486298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.33925/1683-3759-2023-825
A. Kulikov, S. Zhad’ko, I. Romanenko, O. A. Neprelyuk, T. Makalish, M. Kriventsov
Relevance. Despite the high success rate of dental implantation in restoring anatomical occlusion and achieving desired aesthetic effects, complications, often mediated by peri-implantitis, remain an area requiring further study. This study aimed to morphologically assess gingiva biopsy samples from the edentulous alveolar ridge of the jaw, using immunohistochemical markers for immunophenotyping different subpopulations of inflammatory cells, inter alia.Material and methods. Gingiva biopsy specimens from 25 patients underwent descriptive histological and immunohistochemical analyses, employing markers such as CD3, CD20, TBX21, GATA3, Foxp3, CD68, CD80, CD163, CD1a, and CD83. The study includes clinical-anamnestic data analysis, descriptive histological and immunohistochemical observations, and a quantitative analysis of the abundance of various immunopositive cell subpopulations.Results. The data indicated that approximately one third of the gum tissues exhibited signs of a chronic inflammatory response, predominantly characterized by the primary T-cell subpopulation of T-helper 1 cells, a minimal presence of regulatory T-cells, and an almost complete absence of T-helper 2 cells. Additionally, an inflammatory cell mix was observed, comprising a significant proportion of B-cells, monocyte-macrophage cells (mainly M2 macrophage subpopulation), and mature CD83+ dendritic cells. These changes reflect a pro-inflammatory local immune response, crucial to consider in future implantation planning.Conclusion. The analysis of this pilot clinical case series enhances our understanding of the gingiva condition in the edentulous alveolar ridge before dental implantation and opens avenues for further research in this field.
{"title":"Immunohistochemical profile of gingiva biopsy samples from the edentulous alveolar ridge in the jaw","authors":"A. Kulikov, S. Zhad’ko, I. Romanenko, O. A. Neprelyuk, T. Makalish, M. Kriventsov","doi":"10.33925/1683-3759-2023-825","DOIUrl":"https://doi.org/10.33925/1683-3759-2023-825","url":null,"abstract":"Relevance. Despite the high success rate of dental implantation in restoring anatomical occlusion and achieving desired aesthetic effects, complications, often mediated by peri-implantitis, remain an area requiring further study. This study aimed to morphologically assess gingiva biopsy samples from the edentulous alveolar ridge of the jaw, using immunohistochemical markers for immunophenotyping different subpopulations of inflammatory cells, inter alia.Material and methods. Gingiva biopsy specimens from 25 patients underwent descriptive histological and immunohistochemical analyses, employing markers such as CD3, CD20, TBX21, GATA3, Foxp3, CD68, CD80, CD163, CD1a, and CD83. The study includes clinical-anamnestic data analysis, descriptive histological and immunohistochemical observations, and a quantitative analysis of the abundance of various immunopositive cell subpopulations.Results. The data indicated that approximately one third of the gum tissues exhibited signs of a chronic inflammatory response, predominantly characterized by the primary T-cell subpopulation of T-helper 1 cells, a minimal presence of regulatory T-cells, and an almost complete absence of T-helper 2 cells. Additionally, an inflammatory cell mix was observed, comprising a significant proportion of B-cells, monocyte-macrophage cells (mainly M2 macrophage subpopulation), and mature CD83+ dendritic cells. These changes reflect a pro-inflammatory local immune response, crucial to consider in future implantation planning.Conclusion. The analysis of this pilot clinical case series enhances our understanding of the gingiva condition in the edentulous alveolar ridge before dental implantation and opens avenues for further research in this field.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139620411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.33925/1683-3759-2023-845
M. A. Morozov, A. K. Iordanishvili, A. Serikov
Relevance. There is a scarcity of data regarding the treatment of chronic peri-implantitis in HIV-positive patients. The refinement of available techniques for managing inflammation around dental implants holds significant importance, particularly in individuals infected with HIV. Hence, this study aims to consolidate clinical experiences in managing chronic peri-implantitis in HIV-positive patients.Materials and methods. The study involved six male patients (treatment group), aged 35 to 54, diagnosed with chronic peri-implantitis, exhibiting inflammation around dental implants and testing HIV positive. These patients, under dynamic observation by their infectious disease specialist, were consistently receiving antiretroviral treatment. The control group consisted of 10 males aged 28 to 55, devoid of HIV or any other condition causing secondary immunodeficiency. Oral cavity microbiota and mucosal immunity were examined, and treatment for chronic peri-implantitis was administered. Both groups had dental implants for 5 to 7 years. The specifics of the implantation systems were not explored.Results. HIV-positive patients with peri-implantitis exhibited alterations in microbiota and mucosal immunity, correlating with "viral load" indicators. The study presented combined treatment outcomes for periodontitis and the influence of adjusted antiretroviral therapy on mucosal immunity and oral cavity microbiota parameters.Conclusion. Peri-implantitis onset in HIV-positive patients with adequate oral hygiene might be linked to an underlying factor involving ineffective administration of antiretroviral treatment. Consequently, diagnosing periimplantitis in HIV-positive individuals should not only entail appropriate therapeutic and preventive measures as part of a comprehensive peri-implantitis treatment regimen, along with precise oral hygiene correction performed professionally, but also timely referral to an infectious disease specialist for adjustment of antiretroviral therapy.
相关性。有关治疗 HIV 阳性患者慢性种植体周围炎的数据很少。完善现有的牙科种植体周围炎症治疗技术具有重要意义，尤其是对艾滋病病毒感染者而言。因此，本研究旨在总结治疗 HIV 阳性患者慢性种植体周围炎的临床经验。研究涉及六名男性患者（治疗组），年龄在 35 至 54 岁之间，被诊断为慢性种植体周围炎，表现为种植体周围炎症，HIV 检测呈阳性。这些患者接受传染病专家的动态观察，并持续接受抗逆转录病毒治疗。对照组由 10 名男性组成，年龄在 28 至 55 岁之间，没有感染艾滋病毒，也没有任何其他导致继发性免疫缺陷的疾病。他们接受了口腔微生物群和粘膜免疫检查，并接受了慢性种植体周围炎治疗。两组患者均已种植牙 5 至 7 年。结果。种植体周围炎的 HIV 阳性患者表现出微生物群和粘膜免疫的改变，这与 "病毒载量 "指标相关。该研究介绍了牙周炎的综合治疗结果以及调整后的抗逆转录病毒疗法对粘膜免疫和口腔微生物群参数的影响。在口腔卫生良好的艾滋病病毒阳性患者中，种植体周围炎的发病可能与抗逆转录病毒治疗无效的潜在因素有关。因此，在诊断艾滋病病毒阳性患者的种植体周围炎时，不仅要采取适当的治疗和预防措施，将其作为种植体周围炎综合治疗方案的一部分，同时以专业方式进行精确的口腔卫生矫正，还要及时转诊至传染病专科医生，以调整抗逆转录病毒疗法。
{"title":"The experience of treating chronic peri-implantitis in HIV positive patients","authors":"M. A. Morozov, A. K. Iordanishvili, A. Serikov","doi":"10.33925/1683-3759-2023-845","DOIUrl":"https://doi.org/10.33925/1683-3759-2023-845","url":null,"abstract":"Relevance. There is a scarcity of data regarding the treatment of chronic peri-implantitis in HIV-positive patients. The refinement of available techniques for managing inflammation around dental implants holds significant importance, particularly in individuals infected with HIV. Hence, this study aims to consolidate clinical experiences in managing chronic peri-implantitis in HIV-positive patients.Materials and methods. The study involved six male patients (treatment group), aged 35 to 54, diagnosed with chronic peri-implantitis, exhibiting inflammation around dental implants and testing HIV positive. These patients, under dynamic observation by their infectious disease specialist, were consistently receiving antiretroviral treatment. The control group consisted of 10 males aged 28 to 55, devoid of HIV or any other condition causing secondary immunodeficiency. Oral cavity microbiota and mucosal immunity were examined, and treatment for chronic peri-implantitis was administered. Both groups had dental implants for 5 to 7 years. The specifics of the implantation systems were not explored.Results. HIV-positive patients with peri-implantitis exhibited alterations in microbiota and mucosal immunity, correlating with \"viral load\" indicators. The study presented combined treatment outcomes for periodontitis and the influence of adjusted antiretroviral therapy on mucosal immunity and oral cavity microbiota parameters.Conclusion. Peri-implantitis onset in HIV-positive patients with adequate oral hygiene might be linked to an underlying factor involving ineffective administration of antiretroviral treatment. Consequently, diagnosing periimplantitis in HIV-positive individuals should not only entail appropriate therapeutic and preventive measures as part of a comprehensive peri-implantitis treatment regimen, along with precise oral hygiene correction performed professionally, but also timely referral to an infectious disease specialist for adjustment of antiretroviral therapy.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139529517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-11DOI: 10.33925/1683-3759-2024-806
A. S. Zykova, A. S. Opravin, A. G. Soloviev
Relevance. The high prevalence of inflammatory periodontal diseases (IPD) and periodontitis, in particular, is a relevant issue since the latter progression leads to alveolar bone destruction and loss of periodontal attachment and is considered one of the principal causes of tooth loss in the adult population. The adverse impact of periodontal pathogens doesn't only extend to the oral organs and tissues, but it also affects general health. There is evidence of a possible relationship between IPD and cardiovascular diseases (CVD). Periodontal pathogens are found in the myocardium, pericardial fluid, heart valves and vascular intima.Purpose. The study aimed to systematically review the impact of periodontitis on the risk of cardiovascular disease development.Material and methods. The study systematically reviewed scientific articles and original studies from international and national databases using the PRISMA (The Preferred Reporting Items for Systematic Reviews and Meta-Analyses) checklist for systematic reviews and meta-analyses.Results. The study detected 1857 articles according to the inclusion criteria. After the duplicate removal, the number of articles decreased to 685. Of these, 137 articles were selected for screening, which contained data from randomized controlled clinical trials, longitudinal studies, cohort studies and case-control studies. Ninety-eight articles were excluded for the lack of clinical diagnoses or clear diagnostic criteria for the studies and the lack of sample representativeness. The experts assessed 39 articles, of which seven were removed due to a high risk of bias. Finally, 32 studies were included in the systematic review.Conclusion. Thus, the review confirms the hypothesis that there is a relationship between IPD and CVD, such as ACVD, myocardial infarction, peripheral vascular disease, and hypertension. The theory of the pathogenic influence of systemic inflammation caused by the spread of periodontal pathogens in the bloodstream is the key option for grounding this relationship.
{"title":"Periodontitis as a risk factor for cardiovascular disease development: systematic review","authors":"A. S. Zykova, A. S. Opravin, A. G. Soloviev","doi":"10.33925/1683-3759-2024-806","DOIUrl":"https://doi.org/10.33925/1683-3759-2024-806","url":null,"abstract":"Relevance. The high prevalence of inflammatory periodontal diseases (IPD) and periodontitis, in particular, is a relevant issue since the latter progression leads to alveolar bone destruction and loss of periodontal attachment and is considered one of the principal causes of tooth loss in the adult population. The adverse impact of periodontal pathogens doesn't only extend to the oral organs and tissues, but it also affects general health. There is evidence of a possible relationship between IPD and cardiovascular diseases (CVD). Periodontal pathogens are found in the myocardium, pericardial fluid, heart valves and vascular intima.Purpose. The study aimed to systematically review the impact of periodontitis on the risk of cardiovascular disease development.Material and methods. The study systematically reviewed scientific articles and original studies from international and national databases using the PRISMA (The Preferred Reporting Items for Systematic Reviews and Meta-Analyses) checklist for systematic reviews and meta-analyses.Results. The study detected 1857 articles according to the inclusion criteria. After the duplicate removal, the number of articles decreased to 685. Of these, 137 articles were selected for screening, which contained data from randomized controlled clinical trials, longitudinal studies, cohort studies and case-control studies. Ninety-eight articles were excluded for the lack of clinical diagnoses or clear diagnostic criteria for the studies and the lack of sample representativeness. The experts assessed 39 articles, of which seven were removed due to a high risk of bias. Finally, 32 studies were included in the systematic review.Conclusion. Thus, the review confirms the hypothesis that there is a relationship between IPD and CVD, such as ACVD, myocardial infarction, peripheral vascular disease, and hypertension. The theory of the pathogenic influence of systemic inflammation caused by the spread of periodontal pathogens in the bloodstream is the key option for grounding this relationship.","PeriodicalId":509759,"journal":{"name":"Parodontologiya","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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