Pub Date : 2022-01-01DOI: 10.1177/22808000221095232
Naeemeh Sadat Saleh, S. Ostad Movahed, F. Attarbashi
The previous research showed the anti-biofouling improvement of the polyamide six (PA6) fiber when grafted by 2-hydroxyethyl methacrylate (HEMA) individually, and in combination with acrylic acid (AA) known as dual monomers system. The effects of several operative parameters, including vessel reaction temperature and time, ultra violet (UV) pre-irradiation time, and also HEMA-AA mole ratios (for dual system) on the surface grafting of a PA6 fiber by HEMA and also, dual HEMA-AA system were studied. The studied grafting parameters were grafting degree (D), grafting efficiency (E), formed homo and or co-polymer (Y), reaction extent (K), and reaction ratio (R). A pre-UV irradiation technique (PUVA) used for irradiation of the fibers. The results showed efficient polymerization and grafting reactions for the selected operative parameters. The increasing reaction temperature reduced the grafting of the HEMA and AA on the fiber surface. Instead, it accelerated the homo and or co-polymerization of the HEMA and AA monomers. The reaction time had the lowest effect on the homo and co-polymer formation of the used monomers in the studied dual HEMA-AA system. The increasing pre-UV irradiation time was beneficial for the grafting of the studied monomers on the PA6 fiber. The reason referred to increasing the number and concentration of the active sites on the fiber surface.
{"title":"The effects of several operative parameters on the grafting of selected grafting agents on a polyamide six (PA6) fiber surface","authors":"Naeemeh Sadat Saleh, S. Ostad Movahed, F. Attarbashi","doi":"10.1177/22808000221095232","DOIUrl":"https://doi.org/10.1177/22808000221095232","url":null,"abstract":"The previous research showed the anti-biofouling improvement of the polyamide six (PA6) fiber when grafted by 2-hydroxyethyl methacrylate (HEMA) individually, and in combination with acrylic acid (AA) known as dual monomers system. The effects of several operative parameters, including vessel reaction temperature and time, ultra violet (UV) pre-irradiation time, and also HEMA-AA mole ratios (for dual system) on the surface grafting of a PA6 fiber by HEMA and also, dual HEMA-AA system were studied. The studied grafting parameters were grafting degree (D), grafting efficiency (E), formed homo and or co-polymer (Y), reaction extent (K), and reaction ratio (R). A pre-UV irradiation technique (PUVA) used for irradiation of the fibers. The results showed efficient polymerization and grafting reactions for the selected operative parameters. The increasing reaction temperature reduced the grafting of the HEMA and AA on the fiber surface. Instead, it accelerated the homo and or co-polymerization of the HEMA and AA monomers. The reaction time had the lowest effect on the homo and co-polymer formation of the used monomers in the studied dual HEMA-AA system. The increasing pre-UV irradiation time was beneficial for the grafting of the studied monomers on the PA6 fiber. The reason referred to increasing the number and concentration of the active sites on the fiber surface.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45269262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/22808000221105332
Qihao Bian, Junying Chen, Y. Weng, Suiyan Li
In recent years, more and more metal or non-metal materials have been used in the treatment of cardiovascular diseases, but the vascular complications after transplantation are still the main factors restricting the clinical application of most grafts, such as acute thrombosis and graft restenosis. Implant materials have been extensively designed and surface optimized by researchers, but it is still too difficult to avoid complications. Natural vascular endodermis has excellent function, anti-coagulant and anti-intimal hyperplasia, and it is also the key to maintaining the homeostasis of normal vascular microenvironment. Therefore, how to promote the adhesion of endothelial cells (ECs) on the surface of cardiovascular materials to achieve endothelialization of the surface is the key to overcoming the complications after implant materialization. At present, the surface endothelialization design of materials based on materials surface science, bioactive molecules, and biological function intervention and feedback has attracted much attention. In this review, we summarize the related research on the surface modification of materials by endothelialization in recent years, and analyze the advantages and challenges of current endothelialization design ideas, explain the relationship between materials, cells, and vascular remodeling in order to find a more ideal endothelialization surface modification strategy for future researchers to meet the requirements of clinical biocompatibility of cardiovascular materials.
{"title":"Endothelialization strategy of implant materials surface: The newest research in recent 5 years","authors":"Qihao Bian, Junying Chen, Y. Weng, Suiyan Li","doi":"10.1177/22808000221105332","DOIUrl":"https://doi.org/10.1177/22808000221105332","url":null,"abstract":"In recent years, more and more metal or non-metal materials have been used in the treatment of cardiovascular diseases, but the vascular complications after transplantation are still the main factors restricting the clinical application of most grafts, such as acute thrombosis and graft restenosis. Implant materials have been extensively designed and surface optimized by researchers, but it is still too difficult to avoid complications. Natural vascular endodermis has excellent function, anti-coagulant and anti-intimal hyperplasia, and it is also the key to maintaining the homeostasis of normal vascular microenvironment. Therefore, how to promote the adhesion of endothelial cells (ECs) on the surface of cardiovascular materials to achieve endothelialization of the surface is the key to overcoming the complications after implant materialization. At present, the surface endothelialization design of materials based on materials surface science, bioactive molecules, and biological function intervention and feedback has attracted much attention. In this review, we summarize the related research on the surface modification of materials by endothelialization in recent years, and analyze the advantages and challenges of current endothelialization design ideas, explain the relationship between materials, cells, and vascular remodeling in order to find a more ideal endothelialization surface modification strategy for future researchers to meet the requirements of clinical biocompatibility of cardiovascular materials.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48825567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/22808000221092157
C. García-Cuéllar, R. Hernandez-Delgadillo, J. Solís-Soto, I. Meester, Y. Sánchez-Pérez, S. Nakagoshi-Cepeda, M. A. Nakagoshi-Cepeda, S. Chellam, C. Cabral-Romero
Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphology with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. Results: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1 µM. Cytotoxicity was dose-dependent and reached 91% for MCF-7 and 78% for MCF-10A after a 24-h exposure to 100 µM CPC, which outperformed the positive control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6 µM for MCF-7 and 8 µM for MCF-10A, yielding a selectivity index of 1.41. A time response analysis revealed 64% dead cells after only 5 min of exposure to 100 µM CPC. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphology. Conclusion: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5 min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphology nor genotoxicity.
{"title":"Cetylpyridinium chloride inhibits human breast tumor cells growth in a no-selective way","authors":"C. García-Cuéllar, R. Hernandez-Delgadillo, J. Solís-Soto, I. Meester, Y. Sánchez-Pérez, S. Nakagoshi-Cepeda, M. A. Nakagoshi-Cepeda, S. Chellam, C. Cabral-Romero","doi":"10.1177/22808000221092157","DOIUrl":"https://doi.org/10.1177/22808000221092157","url":null,"abstract":"Objective: Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism. Material and methods: The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, the LIVE/DEAD assay, and fluorescence microscopy. Membrane permeability after CPC exposure was evaluated by Calcein AM assay, mitochondrial morphology with a MitoView staining, and genotoxicity with the comet assay and fluorescence microscopy. Results: CPC was cytotoxic to both MCF-7 and MCF-10A as of a 24-h exposure to 0.1 µM. Cytotoxicity was dose-dependent and reached 91% for MCF-7 and 78% for MCF-10A after a 24-h exposure to 100 µM CPC, which outperformed the positive control doxorubicin in effectiveness and selectivity. The LD50 of CPC on was 6 µM for MCF-7 and 8 µM for MCF-10A, yielding a selectivity index of 1.41. A time response analysis revealed 64% dead cells after only 5 min of exposure to 100 µM CPC. With respect to the action mechanisms, the comet assay did not reveal genome fragmentation. On the other hand, membrane damage was dose-dependent and may also affect mitochondrial morphology. Conclusion: Cetylpyridinium chloride inhibits MCF-7 cell growing in a non-selective way as of 5 min of exposure. The action mechanism of CPC on tumor cells involves cell membrane damage without change neither mitochondrial morphology nor genotoxicity.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43891764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/2280800020944015
Zaid A. Al Jeaidi
Aim: This study aims to assess the influence of resin removal treatment regimes on the surface topography and compressive strength of de-bonded ceramic surfaces. Material and methods: Sixty-five lithium disilicate ceramic (LDC) discs were prepared, cleaned, and polished with carbide paper. All samples were etched using 9.6% hydrofluoric acid (HFA). Fifteen samples were taken as positive controls; the remaining 50 samples were subjected to the process of silanization. Resin build-up using dual-cure cement was performed incrementally and light cured. Based on different methods of resin cleaning from de-bonded LDC, the samples were divided into five groups, n=10 each: group 1 (no treatment), group 2 (slow-speed diamond bur), group 3 (1 min heat treatment), Group 4 (6 min heat treatment), and group 5 (sandblasting with Al2O3). Following resin removal, LDC samples were tested under compressive failure load in a universal testing machine. Five disc specimens from each group were sputter coated with gold for scanning electron microscopy (SEM). Analysis of variance (ANOVA) and Tukey’s post hoc test was used for descriptive statistics. Level of significance was established at p<0.05. Results: The highest compressive strength with significant difference among all experimental groups was found in group 5 (321.54 ± 13.25 MPa) (p<0.05). The lowest compressive strength values, presenting significant difference compared with all other groups, were displayed in group 1 (158.57 ± 5.22 MPa) (p<0.05). Compressive strength among group 2 (231.54 ± 15.55 MPa), group 3 (237.81 ± 10.81 MPa), and group 4 (255.53 ± 8.95 MPa) specimens was statistically comparable (p>0.05). On SEM, heat-treated specimens confirmed coarser granules, with mild porosities and roughening, whereas sandblasted specimens exhibited consistent evenness with moderate porosity and loss of glazed surface. Conclusion: De-bonded LDC surface, treated with heat treatment and sandblasting procedures, exhibited removal of residual resin and significantly high compressive strength compared with non-cleansed ceramic surface.
{"title":"Influence of resin removal treatments on the surface topography and strength of de-bonded lithium disilicate ceramic","authors":"Zaid A. Al Jeaidi","doi":"10.1177/2280800020944015","DOIUrl":"https://doi.org/10.1177/2280800020944015","url":null,"abstract":"Aim: This study aims to assess the influence of resin removal treatment regimes on the surface topography and compressive strength of de-bonded ceramic surfaces. Material and methods: Sixty-five lithium disilicate ceramic (LDC) discs were prepared, cleaned, and polished with carbide paper. All samples were etched using 9.6% hydrofluoric acid (HFA). Fifteen samples were taken as positive controls; the remaining 50 samples were subjected to the process of silanization. Resin build-up using dual-cure cement was performed incrementally and light cured. Based on different methods of resin cleaning from de-bonded LDC, the samples were divided into five groups, n=10 each: group 1 (no treatment), group 2 (slow-speed diamond bur), group 3 (1 min heat treatment), Group 4 (6 min heat treatment), and group 5 (sandblasting with Al2O3). Following resin removal, LDC samples were tested under compressive failure load in a universal testing machine. Five disc specimens from each group were sputter coated with gold for scanning electron microscopy (SEM). Analysis of variance (ANOVA) and Tukey’s post hoc test was used for descriptive statistics. Level of significance was established at p<0.05. Results: The highest compressive strength with significant difference among all experimental groups was found in group 5 (321.54 ± 13.25 MPa) (p<0.05). The lowest compressive strength values, presenting significant difference compared with all other groups, were displayed in group 1 (158.57 ± 5.22 MPa) (p<0.05). Compressive strength among group 2 (231.54 ± 15.55 MPa), group 3 (237.81 ± 10.81 MPa), and group 4 (255.53 ± 8.95 MPa) specimens was statistically comparable (p>0.05). On SEM, heat-treated specimens confirmed coarser granules, with mild porosities and roughening, whereas sandblasted specimens exhibited consistent evenness with moderate porosity and loss of glazed surface. Conclusion: De-bonded LDC surface, treated with heat treatment and sandblasting procedures, exhibited removal of residual resin and significantly high compressive strength compared with non-cleansed ceramic surface.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47404690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/22808000221099132
Gang Huang, Pu Li, Gaoyang Li, Jinliang Yang
The study was carried out to explore the biomechanical properties of embracing and non-embracing rib plates. Forty-eight adult cadaver rib specimens were divided randomly into six groups: three fixation model groups were made using embracing plates (two pairs of equals on both sides of the broken end), and the other three groups were fixed with a pre-shaped anatomical plate (three locking screws on each side of the end were equally spaced). The biomechanical properties of these models were analyzed using non-destructive three-point bending tests, non-destructive torsion experiments, and destructive axial compression tests. In this study, the gap of fracture ends was widened in embracing plate group in the non-destructive three-point bending experiment. No change in the fracture ends was detected in the pre-shaped anatomical plate group. The bending stress of the pre-shaped anatomical plate group was significantly enhanced at the 2–12 mm displacement points (p < 0.05). Moreover, there was no significant difference in torque noticed between the two groups in the torsion experiment (p = 0.082). In the destructive axial compression experiment, the load index of the two groups were higher than the normal physiological load, suggesting that both materials could provide sufficient strength for rib fractures. The pre-shaped anatomical plate displayed more reliable attachment in terms of stability, bending, and load. Our results indicated that the embracing plate has the advantage of fretting at the fracture end.
{"title":"Biomechanical study of embracing and non-embracing rib plates","authors":"Gang Huang, Pu Li, Gaoyang Li, Jinliang Yang","doi":"10.1177/22808000221099132","DOIUrl":"https://doi.org/10.1177/22808000221099132","url":null,"abstract":"The study was carried out to explore the biomechanical properties of embracing and non-embracing rib plates. Forty-eight adult cadaver rib specimens were divided randomly into six groups: three fixation model groups were made using embracing plates (two pairs of equals on both sides of the broken end), and the other three groups were fixed with a pre-shaped anatomical plate (three locking screws on each side of the end were equally spaced). The biomechanical properties of these models were analyzed using non-destructive three-point bending tests, non-destructive torsion experiments, and destructive axial compression tests. In this study, the gap of fracture ends was widened in embracing plate group in the non-destructive three-point bending experiment. No change in the fracture ends was detected in the pre-shaped anatomical plate group. The bending stress of the pre-shaped anatomical plate group was significantly enhanced at the 2–12 mm displacement points (p < 0.05). Moreover, there was no significant difference in torque noticed between the two groups in the torsion experiment (p = 0.082). In the destructive axial compression experiment, the load index of the two groups were higher than the normal physiological load, suggesting that both materials could provide sufficient strength for rib fractures. The pre-shaped anatomical plate displayed more reliable attachment in terms of stability, bending, and load. Our results indicated that the embracing plate has the advantage of fretting at the fracture end.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47236500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.1177/2280800020924739
S. Migita, Kosuke Sakashita, Y. Saito, Suyalatu, Tomohiko Yamazaki
The mechanical properties of Co–Cr–Mo (CCM) alloys are advantageous in various biomedical applications. However, because of their bioinert surface, CCM alloys exhibit poor endothelial cell attachment properties; thus, problems of biocompatibility remain. In this study, we aimed to improve the biocompatibility of the CCM alloy surface using solid-binding peptides. We selected peptides with high binding affinity for cast CCM alloy surfaces through in vitro evolution by the phage display method. The peptides were functionalized on the CCM alloy surfaces by simple immersion in the peptide solution. The peptide bound to both cast and 3D-printed CCMs with the same affinity. The peptides linked to the amino acid motif that promotes cell adhesion, and improved the attachment of endothelial cells on the 3D-printed CCM in serum and serum-free conditions. Hence, CCM-binding peptides are attractive tools for constructing a biofunctional surface on CCM-based biodevices.
{"title":"Co–Cr–Mo alloy binding peptide as molecular glue for constructing biomedical surfaces","authors":"S. Migita, Kosuke Sakashita, Y. Saito, Suyalatu, Tomohiko Yamazaki","doi":"10.1177/2280800020924739","DOIUrl":"https://doi.org/10.1177/2280800020924739","url":null,"abstract":"The mechanical properties of Co–Cr–Mo (CCM) alloys are advantageous in various biomedical applications. However, because of their bioinert surface, CCM alloys exhibit poor endothelial cell attachment properties; thus, problems of biocompatibility remain. In this study, we aimed to improve the biocompatibility of the CCM alloy surface using solid-binding peptides. We selected peptides with high binding affinity for cast CCM alloy surfaces through in vitro evolution by the phage display method. The peptides were functionalized on the CCM alloy surfaces by simple immersion in the peptide solution. The peptide bound to both cast and 3D-printed CCMs with the same affinity. The peptides linked to the amino acid motif that promotes cell adhesion, and improved the attachment of endothelial cells on the 3D-printed CCM in serum and serum-free conditions. Hence, CCM-binding peptides are attractive tools for constructing a biofunctional surface on CCM-based biodevices.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/2280800020924739","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46418561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.1177/2280800020926653
M. Mistretta, F. L. La Mantia, Vincenzo Titone, L. Botta, M. Pedeferri, M. Morreale
In this work, the suitability of polylactic acid (PLA), polybutylene adipate terephthalate (PBAT) and PBAT/PLA blend samples to outdoor applications were investigated in terms of mechanical, morphological and visual properties in presence of ultraviolet action and water, finding that PLA in particular can be actually considered for such applications.
{"title":"Effect of ultraviolet and moisture action on biodegradable polymers and their blend","authors":"M. Mistretta, F. L. La Mantia, Vincenzo Titone, L. Botta, M. Pedeferri, M. Morreale","doi":"10.1177/2280800020926653","DOIUrl":"https://doi.org/10.1177/2280800020926653","url":null,"abstract":"In this work, the suitability of polylactic acid (PLA), polybutylene adipate terephthalate (PBAT) and PBAT/PLA blend samples to outdoor applications were investigated in terms of mechanical, morphological and visual properties in presence of ultraviolet action and water, finding that PLA in particular can be actually considered for such applications.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/2280800020926653","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47225672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.1177/2280800019836832
A. Liebermann, D. Vehling, M. Eichberger, B. Stawarczyk
Background: This in-vitro study examined the impact of storage solution, storage duration, and storage temperature on discoloration of three tooth-colored CAD/CAM materials for final restorations. Methods: Specimens (N = 288; n = 96 per material) with a thickness of 1 ± 0.03 mm of the following CAD/CAM materials were fabricated: resin composite (Lava Ultimate, 3M), polymer-infiltrated ceramic (VITA Enamic, VITA Zahnfabrik), and leucite ceramic (IPS Empress CAD, Ivoclar Vivadent). After baseline measurement, specimens were stored in red wine, curry solution, cress solution, and distilled water at 37°C or 55°C. The discoloration was measured using a spectrophotometer (Lambda 35 Perkin Elmer, Perkin Elmer Inc.) after 1 and 7 days storage. Data were analyzed using four-way ANOVA followed by the Scheffé post-hoc test and partial eta squared (ηP²) test (p < 0.05). Results: The highest influence on ∆E was exerted by storage duration (ηP² = 0.295, p < 0.001), followed by storage solution (ηP² = 0.171, p < 0.001), CAD/CAM material (ηP² = 0.049, p < 0.001), and storage temperature (ηP² = 0.033, p < 0.001). Specimens stored for 7 days in staining solutions showed more discoloration than those stored for just 1 day. Higher ∆E values were achieved for specimens stored in curry solution, followed by red wine, cress solution, and distilled water. Resin composite Lava Ultimate showed larger ∆E values compared with the resin hybrid ceramic VITA Enamic and leucite ceramic IPS Empress CAD. Specimens stored at 37°C showed significantly less discoloration than those stored at 55°C. Conclusions: The degree of coloration of the materials depends on food and temperature and was most pronounced with Lava Ultimate.
{"title":"Impact of storage media and temperature on color stability of tooth-colored CAD/CAM materials for final restorations","authors":"A. Liebermann, D. Vehling, M. Eichberger, B. Stawarczyk","doi":"10.1177/2280800019836832","DOIUrl":"https://doi.org/10.1177/2280800019836832","url":null,"abstract":"Background: This in-vitro study examined the impact of storage solution, storage duration, and storage temperature on discoloration of three tooth-colored CAD/CAM materials for final restorations. Methods: Specimens (N = 288; n = 96 per material) with a thickness of 1 ± 0.03 mm of the following CAD/CAM materials were fabricated: resin composite (Lava Ultimate, 3M), polymer-infiltrated ceramic (VITA Enamic, VITA Zahnfabrik), and leucite ceramic (IPS Empress CAD, Ivoclar Vivadent). After baseline measurement, specimens were stored in red wine, curry solution, cress solution, and distilled water at 37°C or 55°C. The discoloration was measured using a spectrophotometer (Lambda 35 Perkin Elmer, Perkin Elmer Inc.) after 1 and 7 days storage. Data were analyzed using four-way ANOVA followed by the Scheffé post-hoc test and partial eta squared (ηP²) test (p < 0.05). Results: The highest influence on ∆E was exerted by storage duration (ηP² = 0.295, p < 0.001), followed by storage solution (ηP² = 0.171, p < 0.001), CAD/CAM material (ηP² = 0.049, p < 0.001), and storage temperature (ηP² = 0.033, p < 0.001). Specimens stored for 7 days in staining solutions showed more discoloration than those stored for just 1 day. Higher ∆E values were achieved for specimens stored in curry solution, followed by red wine, cress solution, and distilled water. Resin composite Lava Ultimate showed larger ∆E values compared with the resin hybrid ceramic VITA Enamic and leucite ceramic IPS Empress CAD. Specimens stored at 37°C showed significantly less discoloration than those stored at 55°C. Conclusions: The degree of coloration of the materials depends on food and temperature and was most pronounced with Lava Ultimate.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/2280800019836832","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43840273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.1177/2280800019872594
A. Yousefi
Treatment of bone defects caused by trauma or disease is a major burden on human healthcare systems. Although autologous bone grafts are considered as the gold standard, they are limited in availability and are associated with post-operative complications. Minimally invasive alternatives using injectable bone cements are currently used in certain clinical procedures, such as vertebroplasty and balloon kyphoplasty. Nevertheless, given the high incidence of fractures and pathologies that result in bone voids, there is an unmet need for injectable materials with desired properties for minimally invasive procedures. This paper provides an overview of the most common injectable bone cement materials for clinical use. The emphasis has been placed on calcium phosphate cements and acrylic bone cements, while enabling the readers to compare the opportunities and challenges for these two classes of bone cements. This paper also briefly reviews antibiotic-loaded bone cements used in bone repair and implant fixation, including their efficacy and cost for healthcare systems. A summary of the current challenges and recommendations for future directions has been brought in the concluding section of this paper.
{"title":"A review of calcium phosphate cements and acrylic bone cements as injectable materials for bone repair and implant fixation","authors":"A. Yousefi","doi":"10.1177/2280800019872594","DOIUrl":"https://doi.org/10.1177/2280800019872594","url":null,"abstract":"Treatment of bone defects caused by trauma or disease is a major burden on human healthcare systems. Although autologous bone grafts are considered as the gold standard, they are limited in availability and are associated with post-operative complications. Minimally invasive alternatives using injectable bone cements are currently used in certain clinical procedures, such as vertebroplasty and balloon kyphoplasty. Nevertheless, given the high incidence of fractures and pathologies that result in bone voids, there is an unmet need for injectable materials with desired properties for minimally invasive procedures. This paper provides an overview of the most common injectable bone cement materials for clinical use. The emphasis has been placed on calcium phosphate cements and acrylic bone cements, while enabling the readers to compare the opportunities and challenges for these two classes of bone cements. This paper also briefly reviews antibiotic-loaded bone cements used in bone repair and implant fixation, including their efficacy and cost for healthcare systems. A summary of the current challenges and recommendations for future directions has been brought in the concluding section of this paper.","PeriodicalId":51074,"journal":{"name":"Journal of Applied Biomaterials & Biomechanics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/2280800019872594","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48901449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.1177/2280800019848923
J. Crisóstomo, A. Pereira, S. Bidarra, A. Gonçalves, P. Granja, Jorge F. J. Coelho, C. Barrias, Raquel Seiça
Introduction: The success of a bioartificial pancreas crucially depends on ameliorating encapsulated beta cells survival and function. By mimicking the cellular in vivo niche, the aim of this study was to develop a novel model for beta cells encapsulation capable of establishing an appropriate microenvironment that supports interactions between cells and extracellular matrix (ECM) components. Methods: ECM components (Arg-Gly-Asp, abbreviated as RGD) were chemically incorporated in alginate hydrogels (alginate-RGD). After encapsulation, INS-1E beta cells outcome was analyzed in vitro and after their implantation in an animal model of diabetes. Results: Our alginate-RGD model demonstrated to be a good in vitro niche for supporting beta cells viability, proliferation, and activity, namely by improving the key feature of insulin secretion. RGD peptides promoted cell–matrix interactions, enhanced endogenous ECM components expression, and favored the assembly of individual cells into multicellular spheroids, an essential configuration for proper beta cell functioning. In vivo, our pivotal model for diabetes treatment exhibited an improved glycemic profile of type 2 diabetic rats, where insulin secreted from encapsulated cells was more efficiently used. Conclusions: We were able to successfully introduce a novel valuable function in an old ally in biomedical applications, the alginate. The proposed alginate-RGD model stands out as a promising approach to improve beta cells survival and function, increasing the success of this therapeutic strategy, which might greatly improve the quality of life of an increasing number of diabetic patients worldwide.
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