Pub Date : 2024-07-01DOI: 10.1016/j.jbc.2024.107583
Magdalena B. Murray, Scott J. Dixon
{"title":"Ferroptosis regulation by Cap’n’collar family transcription factors","authors":"Magdalena B. Murray, Scott J. Dixon","doi":"10.1016/j.jbc.2024.107583","DOIUrl":"https://doi.org/10.1016/j.jbc.2024.107583","url":null,"abstract":"","PeriodicalId":51075,"journal":{"name":"Journal of Biological Chemistry","volume":"52 2","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141694279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.1016/j.jbc.2024.107386
Meghan E. O’Keefe, George Dubyak, Derek W. Abbott
{"title":"Post-translational control of NLRP3 inflammasome signaling","authors":"Meghan E. O’Keefe, George Dubyak, Derek W. Abbott","doi":"10.1016/j.jbc.2024.107386","DOIUrl":"https://doi.org/10.1016/j.jbc.2024.107386","url":null,"abstract":"","PeriodicalId":51075,"journal":{"name":"Journal of Biological Chemistry","volume":"27 16","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141047162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.1016/j.jbc.2024.107387
H. Duan, Huilin Li
{"title":"Consensus, controversies, and conundrums of P4-ATPases: the emerging face of eukaryotic lipid flippases","authors":"H. Duan, Huilin Li","doi":"10.1016/j.jbc.2024.107387","DOIUrl":"https://doi.org/10.1016/j.jbc.2024.107387","url":null,"abstract":"","PeriodicalId":51075,"journal":{"name":"Journal of Biological Chemistry","volume":"165 3","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141050309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.1016/j.jbc.2024.107382
Yang Dong, Jiali Wang, Christof Grewer
{"title":"Transient kinetics reveal the mechanism of competitive inhibition of the neutral amino acid transporter ASCT2","authors":"Yang Dong, Jiali Wang, Christof Grewer","doi":"10.1016/j.jbc.2024.107382","DOIUrl":"https://doi.org/10.1016/j.jbc.2024.107382","url":null,"abstract":"","PeriodicalId":51075,"journal":{"name":"Journal of Biological Chemistry","volume":"18 11","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141036503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-06DOI: 10.1101/2023.05.15.540839
Weiran Feng, Erik Ladewig, Nazifa Salsabeel, Huiyong Zhao, Young Sun Lee, Anuradha Gopalan, Matthew Lange, Hanzhi Luo, Wenfei Kang, Ning Fan, Eric Rosiek, Elisa de Stanchina, Yu Chen, Brett S Carver, Christina S Leslie, Charles L Sawyers
To gain insight into how ERG translocations cause prostate cancer, we performed single cell transcriptional profiling of an autochthonous mouse model at an early stage of disease initiation. Despite broad expression of ERG in all prostate epithelial cells, proliferation was enriched in a small, stem-like population with mixed-luminal basal identity (called intermediate cells). Through a series of lineage tracing and primary prostate tissue transplantation experiments, we find that tumor initiating activity resides in a subpopulation of basal cells that co-express the luminal genes Tmprss2 and Nkx3.1 (called BasalLum) but not in the larger population of classical Krt8+ luminal cells. Upon ERG activation, BasalLum cells give rise to the highly proliferative intermediate state, which subsequently transitions to the larger population of Krt8+ luminal cells characteristic of ERG-positive human cancers. Furthermore, this proliferative population is characterized by an ERG-specific chromatin state enriched for NFkB, AP-1, STAT and NFAT binding, with implications for TF cooperativity. The fact that the proliferative potential of ERG is enriched in a small stem-like population implicates the chromatin context of these cells as a critical variable for unmasking its oncogenic activity.
{"title":"ERG activates a stem-like proliferation-differentiation program in prostate epithelial cells with mixed basal-luminal identity.","authors":"Weiran Feng, Erik Ladewig, Nazifa Salsabeel, Huiyong Zhao, Young Sun Lee, Anuradha Gopalan, Matthew Lange, Hanzhi Luo, Wenfei Kang, Ning Fan, Eric Rosiek, Elisa de Stanchina, Yu Chen, Brett S Carver, Christina S Leslie, Charles L Sawyers","doi":"10.1101/2023.05.15.540839","DOIUrl":"10.1101/2023.05.15.540839","url":null,"abstract":"<p><p>To gain insight into how ERG translocations cause prostate cancer, we performed single cell transcriptional profiling of an autochthonous mouse model at an early stage of disease initiation. Despite broad expression of ERG in all prostate epithelial cells, proliferation was enriched in a small, stem-like population with mixed-luminal basal identity (called intermediate cells). Through a series of lineage tracing and primary prostate tissue transplantation experiments, we find that tumor initiating activity resides in a subpopulation of basal cells that co-express the luminal genes <i>Tmprss2</i> and <i>Nkx3.1</i> (called Basal<sup>Lum</sup>) but not in the larger population of classical <i>Krt8</i>+ luminal cells. Upon ERG activation, Basal<sup>Lum</sup> cells give rise to the highly proliferative intermediate state, which subsequently transitions to the larger population of Krt8+ luminal cells characteristic of ERG-positive human cancers. Furthermore, this proliferative population is characterized by an ERG-specific chromatin state enriched for NFkB, AP-1, STAT and NFAT binding, with implications for TF cooperativity. The fact that the proliferative potential of ERG is enriched in a small stem-like population implicates the chromatin context of these cells as a critical variable for unmasking its oncogenic activity.</p>","PeriodicalId":51075,"journal":{"name":"Journal of Biological Chemistry","volume":"137 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10996491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77892864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.1016/j.jbc.2024.107317
Emma Bose, Shengwei Xiong, Alisha N. Jones
{"title":"Probing RNA Structure and Dynamics using Nanopore and Next Generation Sequencing","authors":"Emma Bose, Shengwei Xiong, Alisha N. Jones","doi":"10.1016/j.jbc.2024.107317","DOIUrl":"https://doi.org/10.1016/j.jbc.2024.107317","url":null,"abstract":"","PeriodicalId":51075,"journal":{"name":"Journal of Biological Chemistry","volume":"495 ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140763631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.1016/j.jbc.2024.107323
Daniel Beaudet, Christopher L. Berger, Adam G. Hendricks
{"title":"The types and numbers of kinesins and dyneins transporting endocytic cargoes modulate their motility and response to tau","authors":"Daniel Beaudet, Christopher L. Berger, Adam G. Hendricks","doi":"10.1016/j.jbc.2024.107323","DOIUrl":"https://doi.org/10.1016/j.jbc.2024.107323","url":null,"abstract":"","PeriodicalId":51075,"journal":{"name":"Journal of Biological Chemistry","volume":"29 1","pages":""},"PeriodicalIF":4.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140770464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}