Journal of Advances in Microbiology最新文献

{"title":"Development of Pink Pigmented Facultative Methylotrophs’ (PPFMs) Consortium Formulation and Its Efficacy on Chilli (Capsicum annuum)","authors":"Solanki J. P., Vyas R. V., Jhala Y. K, Patel H. K.","doi":"10.9734/jamb/2024/v24i3801","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i3801","url":null,"abstract":"This research aims to investigate the potential effect of Pink Pigmented Facultative Methylotrophs (PPFMs) as foliar application to enhance growth parameters of chiili crop. The laboratory and pot studies were conducted at Department of Agricultural Microbiology, Anand Agricultural University, Anand. The PPFMs were isolated from the phyllosphere of solanaceous crop (Chilli, Brinjal, Potato, Tomato and Tobacco) and five out of thirty isolates were selected based on their methanol consuming and plant growth promoting traits. Consortium of isolates (Methylobacterium populi AAU PPFM C-7, M. radiotolerans AAU PPFM C-17, M. populi AAU PPFM C-19, M. populi AAU PPFM T-2 and M. radiotolerans B-2) were prepared after studying their compatibility. Pot study was laid out in net house using completely randomized design with four replications on Chilli crop. The results of the study showed significant effect of PPFMs consortium on growth parameters of chilli (viz. shoot length, root length, shoot weight, root weight, number of branches per plant) that could be result of the plant growth promoting traits of PPFMs.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"49 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140411535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Screening of Natural Metarhizium spp. Isolates for Bioinsecticidal and Biofungicidal Activity","authors":"T. Doolotkeldieva, Sagynych Tolonbekova, S. Bobusheva","doi":"10.9734/jamb/2024/v24i2799","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2799","url":null,"abstract":"Aims: To increase conidia production of local Metarhizium spp—strains by selecting the low-cost media to test obtained bioformulation against the sucking pests and fungal plant pathogens.\u0000Study Design:  The phytophages like black bean aphid (Aphis fabae), a scale insect (Aspidiotus nerii) and spider mite (Tetranychus urticae) are economically essential pests in Kyrgyzstan. The current requirement is to use environmentally friendly protection to reduce the number of these pests. The entomopathogenic fungus from the Metarhizium genus was used to reduce harmful pests in vitro and in vivo experiments.\u0000Place and Duration of Study: Department of Plant Protection, Kyrgyz-Turkish Manas University between May 2021 and May 2023.\u0000Methodology: Natural Metarhizium spp. strains were isolated from dead insect bodies of the Lepidoptera and Apidae families. A laboratory bioassay was conducted to evaluate isolated Metarhizium spp.strains against nymphal stages and adults of sucking pests like A. fabae ((Blackfly), A.nerii (Scale insect) and T.urticae (Spider mite). The inhibitory effect of Metarhizium spp.against Fusarium oxysporum, Alternaria spp. and Rhizoctonia solani was detected using a dual culture technique.\u0000Results: The results of in vitro bioassay tests against sucking pests have revealed the LC50 and LT50 values of TLK-1 isolate. The LC50 of this isolate for A. fabae adults was 1 × 106 conidia mL-1; for A.nerii, it was lower - 7 × 105 conidia ml-1; while for adults T.urticae it was higher- 13 × 106 conidia ml-1. Such mortality occurred after 49.63 hours in adults of A. fabae (LT50), after 46.30 hours in A.nerii (LT50), and after 75.87 hours in deutonymph and adults of T. urticae (LT50). Three isolates, TLK-1, TLA-2 and BZТК1, have exhibited biofingicide activity in pathogen fungi like Fusarium oxysporum and Alternaria spp.\u0000Conclusion: The found isolates can be simultaneously used against pathogenic fungi, essential for developing inexpensive biological formulas based on these fungi.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140421520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Responses of Lactiplantibacillus plantarum and Candida glabrata Isolated from Retted Cassava to Acid Stress and Their Influence on Substrate Fermentation for Gari Production","authors":"O. A. Adewara, S. Ogunbanwo, C. O. Adegoke","doi":"10.9734/jamb/2024/v24i2798","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2798","url":null,"abstract":"Aim: Fermentation subject microbial cells to stress, such as, acid stress which can lead to inactivation of cells and consequently death. Microbial cells have developed mechanisms of adapting to these stress conditions. In this study therefore, the physiological and proteomic responses of Lactiplantibacillus plantarumLC03 and Candida glabrataYC02 to acid stress and their influence on substrate fermentation for Gari production has been investigated. \u0000Methodology: Using the turbidimetry method and SDS-PAGE; LC-MS/MS, the physiological and proteomic responses of the LAB and yeast to acid stress were assessed. Analysis of the physiocochemical and organoleptic properties of the fermented cassava using the LAB and yeast alone and in combination was conducted by means of standard methods. \u0000Results: Lactiplantibacillus plantarumLC03 and Candida glabrataYC02 had growth at pH 1, 2 and pH 3 respectively with an increased protein intensity of Type I glyceraldehyde-3-phosphate dehydrogenase and enolase 2 respectively. The lowest cyanide content (6.49d), highest protein content (0.94c) and improved organoleptic acceptability (7.92a) was observed in Gari produced with the combination of Lactiplantibacillus plantarumLC03 and Candida glabrataYC02 as starters with significant differences in Gari produced with single starters of Lactiplantibacillus plantarumLC03 and Candida glabrataYC02 and control. \u0000Conclusion: Increased protein intensity during acid stress conditions enhanced the survival of Lactiplantibacillus plantarumLC03 and Candida glabrataYC02 (starters), thereby, improving the quality (improved sensory properties, nutritional and reduced anti-nutrient contents) of Gari produced.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"13 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140419352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Urine on Antimicrobial Gradient and Disk Diffusion Susceptibility Testing of Co-trimoxazole, Fosfomycin, Norfloxacin and Nitrofurantoin against Urinary Escherichia coli","authors":"Anju Kuriakose, Arathy Krishna, Gopika Raj R G, Sruthi P, Divya Das, Anila Gopal, Innu S, Roopa Mathews, Harish Kumar K S","doi":"10.9734/jamb/2024/v24i2797","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2797","url":null,"abstract":"Aim: The aim of present study is to examine the influence of urine on zone of inhibition and Minimum inhibitory concentration by antimicrobial gradient strips of Co-trimoxazole, Fosfomycin, Norfloxacin and Nitrofurantoin against urinary E. coli. \u0000Study Design: Cross- sectional study. \u0000Place and Duration of Study: Department of Medical Microbiology, School of Medical Education, Kottayam, Kerala, India. Between January 2023 and November 2023. \u0000Methodology: A total of 75 E. coli isolates collected from various diagnostic microbiology laboratories were included in the study. Identification of isolates and antimicrobial susceptibility testing was done. Direct v/s standard zone of inhibition and MIC was determined. The data was statistically analysed using Cohen’s kappa for interrater reliability and Intraclass correlation coefficient for consistency level. \u0000Results: Current study evaluated the effect of urine on zone of inhibition and MIC by disc diffusion and antibiotic gradient testing respectively. The kappa value, a measure of agreement between direct v/s standard zone of inhibition and MIC for Co-trimoxazole, Fosfomycin, Norfloxacin was a perfect 1 (P=.000), indicating complete agreement. While, The kappa value, a measure of agreement between direct and standard MIC determination for Nitrofurantoin, was .67 (P=.000), indicating substantial agreement. \u0000Conclusion: The present study suggest that direct antimicrobial susceptibility testing can be employed for Co-trimoxazole, Fosfomycin, Norfloxacin and Nitrofurantoin which are important drugs in the management of UTI, after further standardization.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140427167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Harvesting Time, Drying Method and Packaging of Cowpea Leaves on Microbial Contamination","authors":"Gitau Jane Wanjiku, Gathungu Geofrey Kingori, Kiramana James Kirim","doi":"10.9734/jamb/2024/v24i2796","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2796","url":null,"abstract":"After harvesting, cowpea leaves have high moisture content which exposes them to microbial growth. Drying is used to reduce the moisture and extend the shelf life. However, dried foods are susceptible to spoilage resulting from many microbial, biological, chemicals and physical reactions. After drying packaging materials used by farmers also expose the dried product to a range of microorganisms due to their different moisture retention capacity. This study aimed at prolonging the keeping quality of the dried cowpea leaves for use during off- season and coming up with information on the best and affordable packaging material that would ensure safety of dried leaves. Data was collected on fungal, bacteria and coliforms. The data was subjected to variance using Statistical Analysis System 9.2 edition and significantly different means separated using LSD at 5%. The combination of   harvesting stage, drying method and packaging material significantly (p<0.05) influenced microbial load (bacterial and fungal), however no coliforms were observed. Open sun-dried cowpea leaves at 21 DAS, packaged in woven and aluminium foil reported a high number of bacterial and fungal counts compared to the kraft packaging. Oven dried cowpea leaves, harvested at 49 days after sowing (DAS), and in kraft paper resulted in the least bacterial and fungal contamination compared to those packaged in woven and aluminium foil. Sun drying and harvesting time after at 21, 35 and 49 DAS contained the highest bacterial and fungal contamination followed by solar drying and the least was recorded in oven drying method. This research shows that correct harvest stage, adoption of oven and solar drying methods and use of correct packaging material will prolong the shelf life of dried cowpea leaves therefore enhancing   food security and food safety.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"159 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140428520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of Chlorpyrifos Insecticide by Bacillus cereus ST06 and Chryseobacterium sp 6024 Isolated from Agricultural Soil, Nigeria","authors":"Emeribe, Chiemeka Elochi, O. Chinedu","doi":"10.9734/jamb/2024/v24i2795","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2795","url":null,"abstract":"Introduction: Indigenous soil bacteria have the potential to degrade the harmful  chlorpyrifos insecticide, this identifies the importance of biodegradation as an eco-friendly method for chemical pollutant cleanup. \u0000Aims: To compare the potential of Bacillus cereus ST06 and Chryseobacterium sp 6024 in biodegrading chlorpyrifos insecticide singly or as a consortium in a liquid medium. \u0000Study Design: Enrichment culture technique was used to evaluate the bacterial potential in  biodegrading chlorpyrifos insecticide. \u0000Place and Duration of Study: Agricultural soil sample containing chlorpyrifos degrading bacteria was obtained from Ukukwa village Amansea Nigeria (6o16' 30'' N and 7o 07'30''E) from depths of 15cm. Experiment was conducted from January till March 2022. \u0000Methodology: In this study, previously isolated and characterized Bacillus cereus ST06 and Chryseobacterium sp 6024 by standard microbiological method based on their phenotypic test, biochemical test, cultural morphology and 16S rRNA sequencing was used for the experiment. Their growth response to 20mg/l and 60mg/l chlorpyrifos in mineral salts medium singly and as a consortium was compared and determined by monitoring the optical density at 600nm at the optimum condition of pH 6.5 and 30oC temperature for 28 days. The residual chlorpyrifos concentration after 28 days was also  compared and determined using Gas Chromatography- Electron Cathode Detector (GC-ECD). \u0000Results: The result showed a significant difference (P< .001) as Bacillus cereus ST06 and Chryseobacterium sp 6024 responded differently to different concentration of chlorpyrifos.  Bacillus cereus ST06 and Chryseobacterium sp 6024 reached maximum growth in medium containing 20mg/l chlorpyrifos with a mean OD of 0.23±0.20 and 0.42±0.02 respectively on  16th day than 60mg/l chlorpyrifos with a mean OD of 0.47±0.02 and 0.81±0.02 respectively on 20th day. The bacterial consortium also reached maximum growth on 20mg/l and 60mg/l of chlorpyrifos with mean OD of 0.21±0.31 and 0.29±0.02 on 20th day respectively. The result of residual chlorpyrifos concentration shows that the bacteria consortium degraded 79 per cent and 78 per cent of 20mg/l and 60mg/l chlorpyrifos respectively, while Bacillus cereus ST06 and Chryseobacterium sp 6024 degraded 63 per cent and 57 per cent of 20mg/l chlorpyrifos and 61 per cent and 37 per cent of 60mg/l chlorpyrifos. \u0000Conclusion: The study shows that bacteria consortium possessed potential to be used in biodegradation of 20mg/l and 60mg/l Chlorpyrifos than the individual isolates. It is therefore recommended that further studies on RNA profiling of each bacterium and synergistic interaction of the bacterial consortium be studied to better understand regulation of genes and individual bacterial roles in degradation chlorpyrifos efficiently.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140435909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Diseases of Laboratory Animals and its monitoring Tools","authors":"Rekha Manivannan, Tamilselvan Chidambaram, Ramani Gopal, King Solomon Ebenezer","doi":"10.9734/jamb/2024/v24i2794","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2794","url":null,"abstract":"Microbial diseases pose significant challenges in experimental research, impacting both animal welfare and research outcomes. The review covers a wide range of microbial pathogens, including bacteria, viruses, fungi, and parasites, that commonly affect laboratory animals. It explores the etiology, pathogenesis, and clinical manifestations associated with these pathogens, highlighting their influence on experimental results. Extensively discussed about the monitoring assays employed for microbial disease detection in experimental animals. Traditional and modern techniques are considered, including microbiological culturing, serological assays, molecular diagnostics, histopathology, and advanced imaging methods. Regular health monitoring programs for laboratory animals, emphasizing the significance of early detection and intervention to prevent disease outbreaks and maintain animal welfare. Emerging trends and technologies in microbial disease monitoring, such as next-generation sequencing, metagenomics, and high-throughput screening, are also explored. This review aims to provide researchers, laboratory animal professionals, and regulatory authorities with a comprehensive resource for understanding microbial diseases in experimental animals and selecting appropriate monitoring assay. For the Scientists and Veterinarians this overall review gives a glimpse for implementing effective monitoring strategies, identifying and managing microbial diseases, ensuring the well-being of laboratory animals. The synthesis of current knowledge and best practices will enhance the scientific rigor and reproducibility of experimental studies involving animals. In conclusion, this review emphasizes the critical importance of microbial disease monitoring in experimental animals. Robust and accurate monitoring assays enable researchers to effectively detect and manage microbial diseases, safeguarding animal health and ensuring reliable research outcomes.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"13 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140442043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health","authors":"U. Maduakor, C. Eleazar, Chidi George Mba, Chiamaka Cynthia Obodochukwu, Chioma Lily Eberechukwu, C. O. Ogu","doi":"10.9734/jamb/2024/v24i2791","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2791","url":null,"abstract":"Background: A potential threat to public health is the rapidly spreading enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae which produce metallo-beta-lactamases (MBL). This study evaluated the prevalence of metallo-beta-lactamase (MBL) from clinical and non-clinical sources in Enugu Metropolis. \u0000Methodology: The study was conducted in the Microbiology Laboratory of the University of Nigeria Teaching Hospital, Ituku-Ozalla between October 2020 and July 2021. A total of 150 isolates including 85 and 65 isolates of Escherichia coli and Klebsiella pneumoniae respectively was recovered. Standard microbiology procedures were used to identify and characterize the isolates. Antimicrobial susceptibility was done using the Kirby-Bauer disc diffusion technique. Phenotypic detection of Metallo-beta-lactamase production was determined using Combined Disk Tests. \u0000Results: Imipenem resistance was detected in 22 (25.9%) isolates of E. coli and 18 (27.7%) isolates of K. pneumoniae. Of the 22 strains of E. coli that were imipenem resistant, 8 (9.4%) and 14 (16.5%) were found to be MBL producers and non-MBL respectively. Of the 18 strains of Klebsiella pneumoniae that were imipenem resistant, 10 (15.4%) were MBL producers and 8 (12.3%) were non-MBL producers. The highest prevalence of MBL was recovered from urine sources in both E. coli and K. pneumonieae. All MBL-producing isolates were multidrug resistant. \u0000Conclusion: The overall prevalence of MBL in this study was 12.0%. Public health is at risk due to the occurrence of metallo-beta-lactamase. Antimicrobial stewardship and the implementation of infection control strategies are required to halt the spread of these resistant bacteria in the environment. The use of antibiotics should be with utmost prudence.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139849874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health","authors":"U. Maduakor, C. Eleazar, Chidi George Mba, Chiamaka Cynthia Obodochukwu, Chioma Lily Eberechukwu, C. O. Ogu","doi":"10.9734/jamb/2024/v24i2791","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2791","url":null,"abstract":"Background: A potential threat to public health is the rapidly spreading enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae which produce metallo-beta-lactamases (MBL). This study evaluated the prevalence of metallo-beta-lactamase (MBL) from clinical and non-clinical sources in Enugu Metropolis. \u0000Methodology: The study was conducted in the Microbiology Laboratory of the University of Nigeria Teaching Hospital, Ituku-Ozalla between October 2020 and July 2021. A total of 150 isolates including 85 and 65 isolates of Escherichia coli and Klebsiella pneumoniae respectively was recovered. Standard microbiology procedures were used to identify and characterize the isolates. Antimicrobial susceptibility was done using the Kirby-Bauer disc diffusion technique. Phenotypic detection of Metallo-beta-lactamase production was determined using Combined Disk Tests. \u0000Results: Imipenem resistance was detected in 22 (25.9%) isolates of E. coli and 18 (27.7%) isolates of K. pneumoniae. Of the 22 strains of E. coli that were imipenem resistant, 8 (9.4%) and 14 (16.5%) were found to be MBL producers and non-MBL respectively. Of the 18 strains of Klebsiella pneumoniae that were imipenem resistant, 10 (15.4%) were MBL producers and 8 (12.3%) were non-MBL producers. The highest prevalence of MBL was recovered from urine sources in both E. coli and K. pneumonieae. All MBL-producing isolates were multidrug resistant. \u0000Conclusion: The overall prevalence of MBL in this study was 12.0%. Public health is at risk due to the occurrence of metallo-beta-lactamase. Antimicrobial stewardship and the implementation of infection control strategies are required to halt the spread of these resistant bacteria in the environment. The use of antibiotics should be with utmost prudence.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"174 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139789914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavior of Listeria monocytogenes during the Shelf-life of Fresh Refrigerated Pesto Sauce, According to Regulation (EU) 2073/2005 and Subsequent Amendments","authors":"Barbara Franceschini, M. Previdi","doi":"10.9734/jamb/2024/v24i2790","DOIUrl":"https://doi.org/10.9734/jamb/2024/v24i2790","url":null,"abstract":"Aims: Ligurian pesto is one of the most popular condiments for pasta. For microbiological purposes, refrigerated pesto sauce is a great matter for concern, as it can be contaminated by microorganisms originating from both raw materials and manufacturing processes. Listeria monocytogenes is a potential microbiological hazards for this product. The regulatory approach (EU) No. 2073/2005, with subsequent amendments and integrations, takes into consideration the concentration of this pathogen in foods and its growing ability. This study is aimed to assess whether fresh pesto sauce can be included among the Ready-To-Eat (RTE) foods that do not support the growth of L. monocytogenes. \u0000Place and Duration of Study: Quality, Safety and Pre-Industrialization Area, Stazione Sperimentale delle Conserve Alimentari- SSICA, Parma, Italy. Study carried out between October 2021 and June 2023. \u0000Methodology: A screening on 48 different types of commercially available fresh pesto sauces was carried out, evaluating physiochemical parameters (pH and aw). For three different pesto sauces with physical-chemical characteristics suitable for L. monocytogenes growth, microbiological analyses were carried out on both non-inoculated and inoculated samples. The inoculum was made using a mixture of L. monocytogenes, to evaluate its behaviour during the pesto shelf-life. \u0000Results: A relevant presence of commercially available pesto sauces supporting the pathogen growth was found. The study highlighted the inability of the inoculated L. monocytogenes to grow in the tested pesto sauces at 7°C and 10°C during the labelled shelf-life (that varied from 9 to 33 days). \u0000Conclusion: The results obtained from the study can be helpful to the food companies producing pesto sauces, to predict the behaviour of the microorganism and to correctly classify the product, in accordance with the reference regulation (EU) No. 2073/2005 and its subsequent amendments.","PeriodicalId":510775,"journal":{"name":"Journal of Advances in Microbiology","volume":"21 S1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139793620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}