Introduction Polycystic ovarian syndrome is an endocrinal disorder comprising multiple signs and symptoms that are associated with hormonal imbalance. The intervention was administered to subjects who were reluctant to go for pharmacological intervention. Methodology The designed study was intended to evaluate the effect of Surah Al Fatiha in combating PCOS pathology. Inclusion of females was from teenage to 45 years of age. Rotterdam criteria were used to diagnose 7 PCOS cases, while 7 healthy controls were recruited from the Mamji Hospital in Karachi's gynecology department. Surah Al Fatiha exposure was given to all the subjects. Exposure commenced on second day of subject’s menstrual cycle (three times daily) till the subsequent menstrual cycle started. Surah Al Fatiha, a pre-recorded version by ‘Qari Mishary Rashid Alafasy’ was used in this study. Biochemical parameters were monitored and evaluated before and after the Surah Al Fatiha therapy. Results revealed significantly decreased levels of hormones in PCOS afflicted females after Surah Al Fatiha exposure. Insulin and TSH were restored to their normal levels while rest of the hormones though decreased but were in normal range prior to the exposure of intervention. Conclusion Surah Al Fatiha exposure induces serenity and reduces stress, thus inducing normalization of TSH, decreased IR and in turn regularizing the menstrual cycle.
导言 多囊卵巢综合征是一种内分泌失调症，由多种症状和体征组成，与内分泌失调有关。本研究对不愿接受药物治疗的受试者进行干预。研究方法 本研究旨在评估《法提哈经》（Surah Al Fatiha）在防治多囊卵巢综合症病理方面的效果。研究对象为十几岁至 45 岁的女性。采用鹿特丹标准诊断出 7 例多囊卵巢综合症病例，并从卡拉奇马姆吉医院妇科招募了 7 名健康对照者。所有受试者都接触了《法提哈经》。接触从受试者月经周期的第二天开始（每天三次），直到随后的月经周期开始。本研究使用了 "Qari Mishary Rashid Alafasy "预先录制的《法提哈经》。在接受 Surah Al Fatiha 治疗前后，对生化指标进行了监测和评估。结果显示，在接受《Surah Al Fatiha》治疗后，患有多囊卵巢综合症的女性体内激素水平明显下降。胰岛素和促甲状腺激素恢复到了正常水平，而其他激素虽然有所下降，但在接受干预前处于正常范围。结论 接触《法提哈经》能使人宁静，减少压力，从而使促肾上腺皮质激素恢复正常，降低体内激素水平，进而使月经周期正常化。
{"title":"Surah Al Fatiha Induced Betterment of Menstrual Cycle and Hormonal Levels of PCOS: Evidence from Clinical Trials","authors":"Zara Sami, Hajra Naz, Jahan Ara Ainuddin","doi":"10.46568/bios.v5i1.92","DOIUrl":"https://doi.org/10.46568/bios.v5i1.92","url":null,"abstract":"Introduction Polycystic ovarian syndrome is an endocrinal disorder comprising multiple signs and symptoms that are associated with hormonal imbalance. The intervention was administered to subjects who were reluctant to go for pharmacological intervention. Methodology The designed study was intended to evaluate the effect of Surah Al Fatiha in combating PCOS pathology. Inclusion of females was from teenage to 45 years of age. Rotterdam criteria were used to diagnose 7 PCOS cases, while 7 healthy controls were recruited from the Mamji Hospital in Karachi's gynecology department. Surah Al Fatiha exposure was given to all the subjects. Exposure commenced on second day of subject’s menstrual cycle (three times daily) till the subsequent menstrual cycle started. Surah Al Fatiha, a pre-recorded version by ‘Qari Mishary Rashid Alafasy’ was used in this study. Biochemical parameters were monitored and evaluated before and after the Surah Al Fatiha therapy. Results revealed significantly decreased levels of hormones in PCOS afflicted females after Surah Al Fatiha exposure. Insulin and TSH were restored to their normal levels while rest of the hormones though decreased but were in normal range prior to the exposure of intervention. Conclusion Surah Al Fatiha exposure induces serenity and reduces stress, thus inducing normalization of TSH, decreased IR and in turn regularizing the menstrual cycle.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"105 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140507786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction Pakistan possesses a larger stone burden in paediatric patients. In the paediatric population, proper and timely diagnosis of renal stones to get the best stone-free rates is essential. This study aims to assess CT-KUB as a diagnostic tool for renal stones in the paediatric population before PCNL. Methodology This is a retrospective, cross-sectional study. Patients between 1 – 15 years with a diagnosis of renal stones were included in the study. Radiological investigations including Ultrasound KUB, X-ray KUB, and before the procedure, CT-KUB were documented. Pre-operative, per-operative, and postoperative complications are used to assess the success of the procedure. The statistical package of Social Sciences 22 was used to enter, sort, and analyze the data. Variables were analyzed in mean ± standard deviation and frequency and percentages. The chi-square test was used to assess the significance of mean differences keeping the p-value as ≤ 0.05. Results The mean age was 4.7 ± 3.8 years with a range of 1 -14 years. After the procedure, 104 (86.6%) were rendered stone-free after the first setting. Only 1 (0.8%) was converted to an open case (Pyelolithotomy). The mean stone size encountered was 1.3 ± 0.7 cm in greatest dimension as measured on the CT scan. Postoperatively, there was a hemoglobin drop of 2.3 ± 0.5 g/dl noted, mean operative time was 94.8 ± 17.4 minutes. Conclusion CT KUB proves to be an important and accurate preoperative diagnostic tool in assessing patients with renal stones in the paediatric population.
{"title":"Assessment of CT-KUB as A Diagnostic Tool for Renal Stone Assessment in the Pediatric Population","authors":"Izhar Ali, Muahmmad Murtaza Azad","doi":"10.46568/bios.v5i1.180","DOIUrl":"https://doi.org/10.46568/bios.v5i1.180","url":null,"abstract":" Introduction Pakistan possesses a larger stone burden in paediatric patients. In the paediatric population, proper and timely diagnosis of renal stones to get the best stone-free rates is essential. This study aims to assess CT-KUB as a diagnostic tool for renal stones in the paediatric population before PCNL. Methodology This is a retrospective, cross-sectional study. Patients between 1 – 15 years with a diagnosis of renal stones were included in the study. Radiological investigations including Ultrasound KUB, X-ray KUB, and before the procedure, CT-KUB were documented. Pre-operative, per-operative, and postoperative complications are used to assess the success of the procedure. The statistical package of Social Sciences 22 was used to enter, sort, and analyze the data. Variables were analyzed in mean ± standard deviation and frequency and percentages. The chi-square test was used to assess the significance of mean differences keeping the p-value as ≤ 0.05. Results The mean age was 4.7 ± 3.8 years with a range of 1 -14 years. After the procedure, 104 (86.6%) were rendered stone-free after the first setting. Only 1 (0.8%) was converted to an open case (Pyelolithotomy). The mean stone size encountered was 1.3 ± 0.7 cm in greatest dimension as measured on the CT scan. Postoperatively, there was a hemoglobin drop of 2.3 ± 0.5 g/dl noted, mean operative time was 94.8 ± 17.4 minutes. Conclusion CT KUB proves to be an important and accurate preoperative diagnostic tool in assessing patients with renal stones in the paediatric population.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"16 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140508391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saman Ejaz, Maryam Raana, Muhammad Essa, Sadaf Zahra, Snovia Ishaq, Uswa Shoaib, Muhammad Irshad, Zain Ul Abideen
Preterm birth complicates at-risk singleton pregnancies, and this is associated with a poor prognosis for pregnant mothers. When comparing cervical cerclage to vaginal progesterone, the rationale is clear, but its effectiveness remains a mystery.It is the goal of this systematic review to shed further light on this well-known conundrum. To find research on preventive vaginal progesterone usage vs cervical cerclage, PubMed, PubMed Central, Medline, Science Direct, and Google Scholar were searched. There were three randomized control trials, three systematic reviews and meta-analyses, two cohort studies, and two conventional reviews. It was determined that both strategies for managing preterm delivery were equally effective in terms of preterm birth prevention when applied to pregnant women. We found that vaginal progesterone had less side effects than cervical cerclage, but it has no influence on a woman's ability to function and does not improve her life expectancy. The systematic reviews and randomized controlled studies unequivocally confirmed these findings. Cervical cerclage was shown to be more risky than preventive vaginal progesterone in a comprehensive study. However, prophylactic application of cervical cerclage still remains a superior treatment modality if serial transvaginal ultrasound scans are pointing towards cervical shortening. Therefore, more research is required to understand this relationship to identify populations at risk.
早产是高危单胎妊娠的并发症，与孕妇的不良预后有关。将宫颈环扎术与阴道黄体酮进行比较，其原理是明确的，但其有效性仍是一个谜。本系统综述的目的是进一步揭示这一众所周知的难题。为了查找有关预防性使用阴道黄体酮与宫颈环扎术的研究，我们检索了 PubMed、PubMed Central、Medline、Science Direct 和 Google Scholar。其中包括三项随机对照试验、三项系统综述和荟萃分析、两项队列研究和两项常规综述。结果表明，这两种早产管理策略在预防孕妇早产方面同样有效。我们发现，与宫颈环扎术相比，阴道黄体酮的副作用较小，但对妇女的机能没有影响，也不会延长其预期寿命。系统综述和随机对照研究明确证实了这些发现。一项综合研究显示，宫颈环扎术比预防性阴道黄体酮的风险更大。然而，如果连续经阴道超声扫描显示宫颈缩短，预防性应用宫颈环扎术仍然是一种更优越的治疗方式。因此，需要进行更多的研究来了解这种关系，以确定高危人群。
{"title":"Comparing Prophylactic Vaginal Progesterone & Cervical Cerclage In Preventing Preterm Birth: A Systematic Review","authors":"Saman Ejaz, Maryam Raana, Muhammad Essa, Sadaf Zahra, Snovia Ishaq, Uswa Shoaib, Muhammad Irshad, Zain Ul Abideen","doi":"10.46568/bios.v5i1.171","DOIUrl":"https://doi.org/10.46568/bios.v5i1.171","url":null,"abstract":"Preterm birth complicates at-risk singleton pregnancies, and this is associated with a poor prognosis for pregnant mothers. When comparing cervical cerclage to vaginal progesterone, the rationale is clear, but its effectiveness remains a mystery.It is the goal of this systematic review to shed further light on this well-known conundrum. To find research on preventive vaginal progesterone usage vs cervical cerclage, PubMed, PubMed Central, Medline, Science Direct, and Google Scholar were searched. There were three randomized control trials, three systematic reviews and meta-analyses, two cohort studies, and two conventional reviews. It was determined that both strategies for managing preterm delivery were equally effective in terms of preterm birth prevention when applied to pregnant women. We found that vaginal progesterone had less side effects than cervical cerclage, but it has no influence on a woman's ability to function and does not improve her life expectancy. The systematic reviews and randomized controlled studies unequivocally confirmed these findings. Cervical cerclage was shown to be more risky than preventive vaginal progesterone in a comprehensive study. However, prophylactic application of cervical cerclage still remains a superior treatment modality if serial transvaginal ultrasound scans are pointing towards cervical shortening. Therefore, more research is required to understand this relationship to identify populations at risk.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"21 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140506880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ambreen Ejaz, Muhammad Jamaluddin, Shariq Bin Yousuf, Muhammad Saad Usmani, Muhammad Tahir, Zunera Fatima, Alvia Saad
Introduction: Surgical site infections are the most common nosocomial infections worldwide and are also the common problem in developing countries. The impact of its prevalence contributes to morbidity and mortality and therefore the need to determine its prevalence is necessary. Finding out the prevalence of surgical site infections and the frequency of risk variables for surgical site infections in patients undergoing abdominal surgeries at Abbasi Shaheed Hospital in Karachi is the aim of this study. Methodology: This descriptive study was carried out from February 22, 2022- August 22, 2022 at the Abbasi Shaheed Hospital in Karachi with a sample size of 157 subjects with abdominal surgery fulfilling the inclusion criteria. Results: In our study, the mean age, length of operation and BMI were 49.87±8.74 years, 2.14±1.87 hours, 29.72±3 respectively with 61.1% of female and 38.9% male subjects. Out of 157 patients, 22 (14%) and 135 (86%) had and did not have surgical site infection. Conclusion: Patients and the healthcare systems are both heavily impacted by the effects of SSIs. A comprehensive strategy addressing pre-, intra-, and postoperative variables is necessary for the prevention of SSI. In order to lower SSI, hospital infection control procedures should be developed in conjunction with the establishment of an ongoing, long-term surveillance system to detect risk factors.
{"title":"Frequency And Risk Factors For Developing Surgical Site Infection In Abbasi Shaheed Hospital","authors":"Ambreen Ejaz, Muhammad Jamaluddin, Shariq Bin Yousuf, Muhammad Saad Usmani, Muhammad Tahir, Zunera Fatima, Alvia Saad","doi":"10.46568/bios.v5i1.186","DOIUrl":"https://doi.org/10.46568/bios.v5i1.186","url":null,"abstract":"Introduction: Surgical site infections are the most common nosocomial infections worldwide and are also the common problem in developing countries. The impact of its prevalence contributes to morbidity and mortality and therefore the need to determine its prevalence is necessary. Finding out the prevalence of surgical site infections and the frequency of risk variables for surgical site infections in patients undergoing abdominal surgeries at Abbasi Shaheed Hospital in Karachi is the aim of this study. Methodology: This descriptive study was carried out from February 22, 2022- August 22, 2022 at the Abbasi Shaheed Hospital in Karachi with a sample size of 157 subjects with abdominal surgery fulfilling the inclusion criteria. Results: In our study, the mean age, length of operation and BMI were 49.87±8.74 years, 2.14±1.87 hours, 29.72±3 respectively with 61.1% of female and 38.9% male subjects. Out of 157 patients, 22 (14%) and 135 (86%) had and did not have surgical site infection. Conclusion: Patients and the healthcare systems are both heavily impacted by the effects of SSIs. A comprehensive strategy addressing pre-, intra-, and postoperative variables is necessary for the prevention of SSI. In order to lower SSI, hospital infection control procedures should be developed in conjunction with the establishment of an ongoing, long-term surveillance system to detect risk factors.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"10 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140508598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ranoo Mal, D. H. Kalhoro, Hasina Baloch, M. S. Kalhoro, Hubdar Ali Kolachi, Habibullah Janyaro, Muhammad Ibrahim, Fayaz Ahmed, A. A. Channa, M. Mangi, Ali Raza Nizamani, Muhammad Anees Memon, Dildar Hussain, Kalhoro
Introduction: Peste des Petits Ruminants (PPR) is a highly contagious and viraldisease primarily affecting goats and sheep, caused by the PPR virus (PPRV), which belongs tothe family paramyxoviridae, and genus morbillivirus. Methodology: A total of 100 bloodsamples (female n=67 and male n=33) of goats from different areas of Thatta district werecollected. Risk factors like area, age, sex and season were analyzed. Clinically affected animalsexhibited high temperature (41oC), anorexia, dullness, lacrimal secretions, and nasal dischargediahhrea starting from 2 to 6 days post infection, hair blow the eyes becomes wet and there ismatting together of the eyelids as well as partial blockage of the nostrils by dried up purulentdischarges. Samples were transported to Central Veterinary Diagnostic Laboratory (CVDL)Tandojam, Sindh for laboratory confirmation. The competitive ELISA was performed to measureantibodies to the PPR virus. Results: The sero-positivity of PPR cases in female were 76.12%(51/67) and male 51.52% (17/33). The highest sero-prevalence was observed in age group of 4-12month 75.56% (34/45). Lowest sero-prevalence was detected in age group of 0-4 month 47.83%(11/23). Highest prevalence of PPR infections were observed in the month of August 70%(21/30), followed by 69.04% (29/42) in the September, the lowest prevalence was 64.28% (18/28)in the July. Conclusion: It is concluded from the present study that female animals were moreaffected than males. The higher infection was recorded during the August. Comparatively, younganimals were more affected than the suckler and adults.
{"title":"Sero-Prevalence of Peste Des Petits Ruminants Among Goats of Different Zones of District Thatta, Sindh","authors":"Ranoo Mal, D. H. Kalhoro, Hasina Baloch, M. S. Kalhoro, Hubdar Ali Kolachi, Habibullah Janyaro, Muhammad Ibrahim, Fayaz Ahmed, A. A. Channa, M. Mangi, Ali Raza Nizamani, Muhammad Anees Memon, Dildar Hussain, Kalhoro","doi":"10.46568/bios.v5i1.172","DOIUrl":"https://doi.org/10.46568/bios.v5i1.172","url":null,"abstract":"Introduction: Peste des Petits Ruminants (PPR) is a highly contagious and viraldisease primarily affecting goats and sheep, caused by the PPR virus (PPRV), which belongs tothe family paramyxoviridae, and genus morbillivirus. Methodology: A total of 100 bloodsamples (female n=67 and male n=33) of goats from different areas of Thatta district werecollected. Risk factors like area, age, sex and season were analyzed. Clinically affected animalsexhibited high temperature (41oC), anorexia, dullness, lacrimal secretions, and nasal dischargediahhrea starting from 2 to 6 days post infection, hair blow the eyes becomes wet and there ismatting together of the eyelids as well as partial blockage of the nostrils by dried up purulentdischarges. Samples were transported to Central Veterinary Diagnostic Laboratory (CVDL)Tandojam, Sindh for laboratory confirmation. The competitive ELISA was performed to measureantibodies to the PPR virus. Results: The sero-positivity of PPR cases in female were 76.12%(51/67) and male 51.52% (17/33). The highest sero-prevalence was observed in age group of 4-12month 75.56% (34/45). Lowest sero-prevalence was detected in age group of 0-4 month 47.83%(11/23). Highest prevalence of PPR infections were observed in the month of August 70%(21/30), followed by 69.04% (29/42) in the September, the lowest prevalence was 64.28% (18/28)in the July. Conclusion: It is concluded from the present study that female animals were moreaffected than males. The higher infection was recorded during the August. Comparatively, younganimals were more affected than the suckler and adults.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"4 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140508276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. A. Channa, Atta Hussain, Ayaz Hussain Mangi, N. H. Kalhoro, Zaheer Ahmed Nizamani, H. Soomro, Ahmed Jamali
Introduction Avian influenza virus (H7N3) cause severe systemic disease in chickens and can be persisted in infected chicken meat that cause public health concern. Influenza viruses are heat liable but parameters for heat inactivation have not been known. Methodology The study investigated the quantitative heat inactivation of H7N3 virus from experimentally infected chicken meat. Twenty (20) sero-negative broiler chickens of 04 weeks age were divided into two group viz A (control) and B artificially infected by giving 0.1ml of 106 embryo infectious dose 50 (EID50) (H7N3) virus through intranasal route. Birds were slaughtered on day 9 post infection and tissues (trachea, kidney, lungs and liver) were collected. Inactivation curves were determined at temperature 60, 61, 62, 63 and 64°C. Results Based on D values, time to inactivate H7N3 virus depends on viral titers and inversely related to temperature. This study investigated confirmed that H7N3 virus was effectively inactivated in chicken meat at 60°C in 4 minutes while at 64°C in 1 minutes. Moreover, protocols per log EID50/g reduction in terms of D values at 60 and 64°C were noted 120 and 30 sec, the coefficient of regression ranged as 0.941 to 0.889.
{"title":"Heat Inactivation of Avian Influenza (H7N3) Virus In Experimentally Infected Chicken Meat","authors":"A. A. Channa, Atta Hussain, Ayaz Hussain Mangi, N. H. Kalhoro, Zaheer Ahmed Nizamani, H. Soomro, Ahmed Jamali","doi":"10.46568/bios.v5i1.163","DOIUrl":"https://doi.org/10.46568/bios.v5i1.163","url":null,"abstract":"Introduction Avian influenza virus (H7N3) cause severe systemic disease in chickens and can be persisted in infected chicken meat that cause public health concern. Influenza viruses are heat liable but parameters for heat inactivation have not been known. Methodology The study investigated the quantitative heat inactivation of H7N3 virus from experimentally infected chicken meat. Twenty (20) sero-negative broiler chickens of 04 weeks age were divided into two group viz A (control) and B artificially infected by giving 0.1ml of 106 embryo infectious dose 50 (EID50) (H7N3) virus through intranasal route. Birds were slaughtered on day 9 post infection and tissues (trachea, kidney, lungs and liver) were collected. Inactivation curves were determined at temperature 60, 61, 62, 63 and 64°C. Results Based on D values, time to inactivate H7N3 virus depends on viral titers and inversely related to temperature. This study investigated confirmed that H7N3 virus was effectively inactivated in chicken meat at 60°C in 4 minutes while at 64°C in 1 minutes. Moreover, protocols per log EID50/g reduction in terms of D values at 60 and 64°C were noted 120 and 30 sec, the coefficient of regression ranged as 0.941 to 0.889.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"9 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140508407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Afsheen Sardar, J. Alam, A. Hussain, S. R. Mahmood, M. Ansari, M. Ansari
Introduction Cardiovascular disease (CVD) is one of the prominent causes of mortality in cases of chronic Type 2 diabetes mellitus (T2DM) patients and necessitates improving risk categorization. There are few available biomarkers that can assess preceding or current glycemic and cardiac status, but not prognosis. Serum homocysteine (Hcy) has been indicated and reported to be a likely biomarker that can detect cardiovascular complication in patients with T2DM. Methodology Present study details the comparative analysis of several biochemical and metabolic biomarkers including Hcy in T2DM patients with and without CVD complications. A total of eighty patients, n = 40 each in T2DM with CVD and T2DM without CVD, were included in the study. Patient’s preparation, blood sample collection and analyses of all biochemical, metabolic markers including Hcy were performed as per standard protocols. One way ANOVA was used for independent measures including Tukey HSD with level of significance at P< 0.05. Results Indication of hyperhomocysteinemia, was significantly apparent in patients with T2DM who have CVD, as compared to those with T2DM without CVD. All other biochemical and metabolic parameters manifested marked significant (P< 0.00001) elevations, which was more perceptible in T2DM CVD as compared to T2DM non CVD. Clinical relevance of high Hcy in blood in patients with T2DM CVD thus suggested being prominent risk factor for proceeding renal and cardiac complications.
{"title":"Indication of Hyperhomocysteinemia in Type 2 Diabetes Mellitus Patients with Cardiovascular Complications","authors":"Afsheen Sardar, J. Alam, A. Hussain, S. R. Mahmood, M. Ansari, M. Ansari","doi":"10.46568/bios.v5i1.149","DOIUrl":"https://doi.org/10.46568/bios.v5i1.149","url":null,"abstract":"Introduction Cardiovascular disease (CVD) is one of the prominent causes of mortality in cases of chronic Type 2 diabetes mellitus (T2DM) patients and necessitates improving risk categorization. There are few available biomarkers that can assess preceding or current glycemic and cardiac status, but not prognosis. Serum homocysteine (Hcy) has been indicated and reported to be a likely biomarker that can detect cardiovascular complication in patients with T2DM. Methodology Present study details the comparative analysis of several biochemical and metabolic biomarkers including Hcy in T2DM patients with and without CVD complications. A total of eighty patients, n = 40 each in T2DM with CVD and T2DM without CVD, were included in the study. Patient’s preparation, blood sample collection and analyses of all biochemical, metabolic markers including Hcy were performed as per standard protocols. One way ANOVA was used for independent measures including Tukey HSD with level of significance at P< 0.05. Results Indication of hyperhomocysteinemia, was significantly apparent in patients with T2DM who have CVD, as compared to those with T2DM without CVD. All other biochemical and metabolic parameters manifested marked significant (P< 0.00001) elevations, which was more perceptible in T2DM CVD as compared to T2DM non CVD. Clinical relevance of high Hcy in blood in patients with T2DM CVD thus suggested being prominent risk factor for proceeding renal and cardiac complications.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"76 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140507820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Javed Anjum, Mustajab A. Khan, J. Babar, F. Ahmed, Meraj Domar, Muhammad Ejaz, T. Ziad, Rehan Fareed, Hidayatullah Kakar
Introduction Soil fertility in Pakistan is hindered by insufficient and unstable organic matter, leading to stagnation and a decline in overall agricultural productivity. Methodology A pot study was conducted in 2018-19 comprised of four treatments including T1 = Recommended NPK fertilizer, T2 = NPK+FYM, T3 = NPK + Poultry manure, and T4 = NPK + Plant residue based on Complete Randomized Design (CRD) and was replicated thrice. Result The results exhibited significant differences in peas’ growth, yield, and nutrient use efficiency across the organic amendments. Among them, the higher plant height (70.63 cm), pod length (9.37 cm), and pod yield (158.63 g pot-1) was observed in T3 followed by T2. Regarding nutrient concentration and uptake, both T2 and T3 treatments elevated leaf tissue levels of N (4.76% and 5.03%), P (1.01% and 0.98%), and K (2.27% and 2.31%). These values were statistically comparable, accompanied by higher but non-significant P uptake (0.61 and 0.67 g pot-1) and K uptake (1.13 and 1.21 g pot-1) for the respective treatments. Further, higher nitrogen, phosphorus, and potassium use efficiency (49.4, 5.20, and 42.0%) were recorded in T3 followed by T2. Together with that significantly positive correlation between soil properties and pod yield was found which reflects the nutrient-supplying power of soil under organic amendments. Conclusion Consequently, it is suggested that the joint utilization of organic additives of any source and chemical fertilizer is necessary for increasing yield and nutrient use efficiency over sole chemical fertilizer application in calcareous soils.
引言 巴基斯坦的土壤肥力因有机质不足和不稳定而受到阻碍，导致停滞不前，整体农业生产力下降。方法 在完全随机设计（CRD）的基础上，于 2018-19 年进行了一项盆栽研究，包括四个处理，包括 T1 = 推荐氮磷钾肥料、T2 = 氮磷钾+FYM、T3 = 氮磷钾+家禽粪便和 T4 = 氮磷钾+植物残渣，并重复三次。结果 结果表明，不同有机添加剂对豌豆的生长、产量和养分利用效率有显著差异。其中，T3 的株高（70.63 厘米）、豆荚长度（9.37 厘米）和豆荚产量（158.63 克/盆-1）均高于 T2。在养分浓度和吸收方面，T2 和 T3 处理都提高了叶片组织中氮（4.76% 和 5.03%）、磷（1.01% 和 0.98%）和钾（2.27% 和 2.31%）的含量。这些数值在统计学上具有可比性，同时，各处理对 P 的吸收量（0.61 和 0.67 g pot-1）和 K 的吸收量（1.13 和 1.21 g pot-1）较高，但不显著。此外，T3 的氮、磷、钾利用率较高（49.4%、5.20% 和 42.0%），其次是 T2。此外，土壤特性与豆荚产量之间存在明显的正相关，这反映了有机添加剂下土壤的养分供应能力。结论 因此，有必要在石灰性土壤中联合使用任何来源的有机添加剂和化肥，以提高产量和养分利用效率，而不是只施用化肥。
{"title":"Improving Pea Growth and Nutrient Efficiency in Calcareous Soils through the Synergy of Organic Amendments and Chemical Fertilizers","authors":"Javed Anjum, Mustajab A. Khan, J. Babar, F. Ahmed, Meraj Domar, Muhammad Ejaz, T. Ziad, Rehan Fareed, Hidayatullah Kakar","doi":"10.46568/bios.v5i1.165","DOIUrl":"https://doi.org/10.46568/bios.v5i1.165","url":null,"abstract":"Introduction Soil fertility in Pakistan is hindered by insufficient and unstable organic matter, leading to stagnation and a decline in overall agricultural productivity. Methodology A pot study was conducted in 2018-19 comprised of four treatments including T1 = Recommended NPK fertilizer, T2 = NPK+FYM, T3 = NPK + Poultry manure, and T4 = NPK + Plant residue based on Complete Randomized Design (CRD) and was replicated thrice. Result The results exhibited significant differences in peas’ growth, yield, and nutrient use efficiency across the organic amendments. Among them, the higher plant height (70.63 cm), pod length (9.37 cm), and pod yield (158.63 g pot-1) was observed in T3 followed by T2. Regarding nutrient concentration and uptake, both T2 and T3 treatments elevated leaf tissue levels of N (4.76% and 5.03%), P (1.01% and 0.98%), and K (2.27% and 2.31%). These values were statistically comparable, accompanied by higher but non-significant P uptake (0.61 and 0.67 g pot-1) and K uptake (1.13 and 1.21 g pot-1) for the respective treatments. Further, higher nitrogen, phosphorus, and potassium use efficiency (49.4, 5.20, and 42.0%) were recorded in T3 followed by T2. Together with that significantly positive correlation between soil properties and pod yield was found which reflects the nutrient-supplying power of soil under organic amendments. Conclusion Consequently, it is suggested that the joint utilization of organic additives of any source and chemical fertilizer is necessary for increasing yield and nutrient use efficiency over sole chemical fertilizer application in calcareous soils.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140507476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Shaikh, Naheed Baloch, A. Arijo, Riffat Sultana, Z. Abdin
Introduction: The Theileria annulata is a haemo protozoan parasite that causes economically significant fatal tick-borne diseases in domesticated and wild animals. Methodology: The research focused on identifying Theileria annulata infection in both cattle and buffalo through molecular methods. To achieve this, DNA extraction was conducted using whole blood samples, followed by the design of specific primers for Theileria annulata and subsequent PCR (Polymerase Chain Reaction) analysis. Results: Data were also collected on tick infestation and host-parasite relationship. A total of 2400 Cattle and Buffaloes were evaluated during the study. District-wise detection indicated that the highest rate of infected samples was recorded from Peri-Urban (Cattle=88.33%, Buffalo= 61.94%) and Urban areas (Cattle=65.38%, Buffalo=54.10%) of district Hyderabad, while the lowest rate was recorded from Peri-urban (Cattle=24%, Buffalo=17.9%) and Urban (20.38%, Buffalo=16.66%) areas of district Karachi, Month wise detection of Theileria annulata indicated the highest rate in July (Cattle= 74.0%, Buffalo=47.5%) and the lowest rate in December (Cattle=11.7%, Buffalo=18.1%). The prevalence of parasitic infection showed a notable increase (P<0.05) in the areas of the Suburban region than in Cityscape areas. Conclusion: In the molecular identification of Theileria annulata, the infection rate exhibited a notable difference between District Hyderabad and District Karachi, with a higher prevalence in the former and a lower incidence in the latter. Additionally, cattle displayed greater susceptibility to tick infestation in comparison to buffalo.
导言：环状蜱虫是一种血原生动物寄生虫，会导致家养和野生动物患上致命的蜱媒疾病，具有重要的经济意义。研究方法：研究重点是通过分子方法确定牛和水牛是否感染了环口丝虫。为此，使用全血样本进行了 DNA 提取，然后设计了环状忒勒氏菌的特异引物，随后进行了聚合酶链式反应（PCR）分析。结果：还收集了有关蜱虫侵扰和宿主与寄生虫关系的数据。研究期间共对 2400 头牛和水牛进行了评估。按地区分列的检测结果表明，海得拉巴地区的城市周边地区（牛=88.33%，水牛=61.94%）和城市地区（牛=65.38%，水牛=54.10%）的样本感染率最高，而城市周边地区（牛=24%，水牛=17.按月份分列的环状丝虫病检测结果表明，7 月份的感染率最高（牛=74.0%，水牛=47.5%），12 月份的感染率最低（牛=11.7%，水牛=18.1%）。城郊地区的寄生虫感染率明显高于城市地区（P<0.05）。结论在环状沙雷氏菌的分子鉴定中，海德拉巴区和卡拉奇区的感染率存在明显差异，前者的感染率较高，后者的感染率较低。此外，与水牛相比，牛更容易受到蜱虫的侵扰。
{"title":"Investigating The Molecular Aspects of Theileria Annulata In Naturally Infected Animals, Alongside A Mention of Tick Distribution In Hyderabad And Karachi","authors":"I. Shaikh, Naheed Baloch, A. Arijo, Riffat Sultana, Z. Abdin","doi":"10.46568/bios.v5i1.128","DOIUrl":"https://doi.org/10.46568/bios.v5i1.128","url":null,"abstract":"Introduction: The Theileria annulata is a haemo protozoan parasite that causes economically significant fatal tick-borne diseases in domesticated and wild animals. Methodology: The research focused on identifying Theileria annulata infection in both cattle and buffalo through molecular methods. To achieve this, DNA extraction was conducted using whole blood samples, followed by the design of specific primers for Theileria annulata and subsequent PCR (Polymerase Chain Reaction) analysis. Results: Data were also collected on tick infestation and host-parasite relationship. A total of 2400 Cattle and Buffaloes were evaluated during the study. District-wise detection indicated that the highest rate of infected samples was recorded from Peri-Urban (Cattle=88.33%, Buffalo= 61.94%) and Urban areas (Cattle=65.38%, Buffalo=54.10%) of district Hyderabad, while the lowest rate was recorded from Peri-urban (Cattle=24%, Buffalo=17.9%) and Urban (20.38%, Buffalo=16.66%) areas of district Karachi, Month wise detection of Theileria annulata indicated the highest rate in July (Cattle= 74.0%, Buffalo=47.5%) and the lowest rate in December (Cattle=11.7%, Buffalo=18.1%). The prevalence of parasitic infection showed a notable increase (P<0.05) in the areas of the Suburban region than in Cityscape areas. Conclusion: In the molecular identification of Theileria annulata, the infection rate exhibited a notable difference between District Hyderabad and District Karachi, with a higher prevalence in the former and a lower incidence in the latter. Additionally, cattle displayed greater susceptibility to tick infestation in comparison to buffalo.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":"52 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140508098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Uzma Hussain, Faryal Kanwal, Muhammad Essa, Uswah Shoiab, Maria Imran, Snovia Ishaq, Huma Khan, Muhammad Irshad, Zain Ul Abideen
Introduction: There is insufficient evidence to support the use of cystoinflation to prevent bladder injury in women with placenta previa. The purpose of this study is to find the effectiveness and safety of cystoinflation to prevent bladder injury in women with placenta previa. Methods: The study was conducted at Gynecology & Obstetrics Department, lady Willing-don hospital, Lahore for six months duration . The research was conducted by forming two groups. Women in Group-A (inflated urinary bladder) had significantly lower bladder injury as compared to women in Group-B (deflated urinary bladder). i.e. 1.9% vs. 11.32%, p-value=0.050. Results: Homeostatic sutures requirement was significantly higher in Group-B as that of Group-A. i.e. 15.1% vs. 18.9%, p-value=0.604 Duration of hospital stay showed no significant difference between groups. i.e. 96.2% patients in Group-A and 88.7% patients in Group-B discharged from hospital within 3-4 days. Conclusion: Based on the results of this study, women presenting with placenta previa had significantly lower incidences of bladder injury as a result of cystoinflation during cesarean section.
导言：目前还没有足够的证据支持使用膀胱充气来预防前置胎盘妇女的膀胱损伤。本研究旨在了解膀胱充气对预防前置胎盘妇女膀胱损伤的有效性和安全性。研究方法该研究在拉合尔威灵顿夫人医院妇产科进行，为期六个月。研究分为两组进行。 A组（膀胱充盈）妇女的膀胱损伤率明显低于B组（膀胱瘪陷）妇女，即1.9% vs. 11.32%，P值=0.050。结果B组所需静态缝合的比例明显高于A组，即15.1% vs. 18.9%，P值=0.604 住院时间显示组间无明显差异，即A组96.2%的患者和B组88.7%的患者在3-4天内出院。结论根据这项研究的结果，患有前置胎盘的产妇在剖腹产过程中因膀胱充气而导致膀胱损伤的发生率明显较低。
{"title":"Use of Cystoinflation to Prevent Urinary Tract Injuries in Patients with Placenta Previa","authors":"Uzma Hussain, Faryal Kanwal, Muhammad Essa, Uswah Shoiab, Maria Imran, Snovia Ishaq, Huma Khan, Muhammad Irshad, Zain Ul Abideen","doi":"10.46568/bios.v5i1.183","DOIUrl":"https://doi.org/10.46568/bios.v5i1.183","url":null,"abstract":"Introduction: There is insufficient evidence to support the use of cystoinflation to prevent bladder injury in women with placenta previa. The purpose of this study is to find the effectiveness and safety of cystoinflation to prevent bladder injury in women with placenta previa. Methods: The study was conducted at Gynecology & Obstetrics Department, lady Willing-don hospital, Lahore for six months duration . The research was conducted by forming two groups. Women in Group-A (inflated urinary bladder) had significantly lower bladder injury as compared to women in Group-B (deflated urinary bladder). i.e. 1.9% vs. 11.32%, p-value=0.050. Results: Homeostatic sutures requirement was significantly higher in Group-B as that of Group-A. i.e. 15.1% vs. 18.9%, p-value=0.604 Duration of hospital stay showed no significant difference between groups. i.e. 96.2% patients in Group-A and 88.7% patients in Group-B discharged from hospital within 3-4 days. Conclusion: Based on the results of this study, women presenting with placenta previa had significantly lower incidences of bladder injury as a result of cystoinflation during cesarean section.","PeriodicalId":516564,"journal":{"name":"BioSight","volume":" 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139640447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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