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[Proteomics analysis of the effect and mechanism of ADSCs on full-thickness skin defects in diabetic rats]. [ADSCs对糖尿病大鼠全层皮肤缺损的作用及机制的蛋白质组学分析]。
Pub Date : 2026-01-20 DOI: 10.3760/cma.j.cn501225-20240617-00236
Y Gu, Y Z Chen, S Y Wang, H Y Song, N Bai
<p><p><b>Objective:</b> To explore the effect and mechanism of adipose-derived mesenchymal stem cells (ADSCs) on full-thickness skin defects in diabetic rats using proteomics analysis. <b>Methods:</b> This study was a self-control design experimental study. Four 8 to 10 weeks old male Sprague-Dawley rats were selected, and ADSCs were extracted from their epididymal adipose tissue and successfully identified. The third passage of ADSCs were used for the following experiments. Twenty-four 4 to 6 weeks old male Sprague-Dawley rats were selected and the type 2 diabetes model was successfully established. Among them, 16 diabetic rats weighing 350 to 400 g were chosen, and a full-thickness skin defect wound was created on each side of the spine on their backs at the same level. Using the random number table method, the two wounds of each rat were included in experimental group and control group (with 16 wounds in each group), and the cell suspensions containing ADSCs and phosphate buffered saline were injected at multiple points around and at the base of the wounds immediately after injury, respectively. The wound healing rates were calculated at day 7, 10, and 14 after injury in rats. At day 7 after injury in rats, wound tissue was collected and proteins were extracted. Four-dimensional data-independent acquisition label-free quantitative proteomics technology was used for quantitative proteomics analysis and bioinformatics analysis to screen differentially expressed proteins (DEPs) in the two groups of wound tissue. Then, key DEPs were screened through protein-protein interaction networks, gene ontology (GO) was used for functional annotation and enrichment analysis, and Kyoto encyclopedia of genes and genomes (KEGG) was used for pathway enrichment analysis to further screen target DEPs. The wound tissue of rats 7 days after injury was collected, and Western blotting was used to detect the protein expression of thymocyte differentiation antigen 1 (Thy-1) and G protein-coupled receptor 177/Wnt ligand secretion mediator (GPR177/Wls). <b>Results:</b> At day 7, 10, and 14 after injury in rats, the wound healing rates in experimental group were (66±16)%, (83±8)%, and (93±4)%, respectively, which were significantly higher than (30±8)%, (62±6)%, and (77±8)% in control group (with <i>t</i> values of -4.41, -7.46, and -6.65, respectively, <i>P</i><0.05). At day 7 after injury in rats, compared with those in control group, a total of 474 DEPs were screened from the wound tissue in experimental group (<i>P</i><0.05). A total of 224 key DEPs were further screened out, among which 78 DEPs were significantly upregulated and 146 DEPs were significantly downregulated. GO functional annotation analysis showed that the most significantly upregulated and downregulated DEPs mainly affected protein expression under cellular processes and biological regulation conditions, and were related to cell anatomical entities and protein-containing complexes, as well as specific bi
目的:通过蛋白质组学分析,探讨脂肪源性间充质干细胞(ADSCs)对糖尿病大鼠全层皮肤缺损的修复作用及其机制。方法:采用自我控制设计实验研究。选取4只8 ~ 10周龄雄性Sprague-Dawley大鼠,从其附睾脂肪组织中提取ADSCs并成功鉴定。第三代ADSCs用于后续实验。选取24只4 ~ 6周龄雄性Sprague-Dawley大鼠,成功建立2型糖尿病模型。其中选取体重350 ~ 400 g的糖尿病大鼠16只,在其背部脊柱两侧同一水平处造全层皮肤缺损创面。采用随机数字表法,将每只大鼠的2个创面分为实验组和对照组(每组16个创面),损伤后立即分别在创面周围和创面底部多点注射含有ADSCs和磷酸盐缓冲盐水的细胞悬液。分别于伤后第7、10、14天计算创面愈合率。大鼠损伤后第7天,收集创面组织,提取蛋白。采用四维数据独立获取无标记定量蛋白质组学技术进行定量蛋白质组学分析和生物信息学分析,筛选两组创面组织中的差异表达蛋白(differential expression protein, DEPs)。然后,通过蛋白-蛋白相互作用网络筛选关键DEPs,利用基因本体(GO)进行功能注释和富集分析,利用京都基因基因组百科全书(KEGG)进行途径富集分析,进一步筛选目标DEPs。取伤后7 d大鼠创面组织,采用Western blotting检测胸腺细胞分化抗原1 (Thy-1)和G蛋白偶联受体177/Wnt配体分泌介质(GPR177/Wls)的蛋白表达。结果:大鼠损伤后第7、10、14天,实验组创面愈合率分别为(66±16)%、(83±8)%、(93±4)%,显著高于对照组的(30±8)%、(62±6)%、(77±8)% (t值分别为-4.41、-7.46、-6.65,PPt值分别为11.61、9.41)。基于蛋白质组学分析发现,大鼠ADSCs可通过上调GPR177/Wls和Thy-1蛋白表达,促进糖尿病大鼠全层皮肤缺损创面愈合。
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引用次数: 0
[Application of pectoralis major fascia in robotic nipple-sparing mastectomy with immediate breast reconstruction with gel implant]. 【胸大肌筋膜在机器人保乳头乳房切除术及即刻凝胶乳房重建中的应用】。
Pub Date : 2026-01-20 DOI: 10.3760/cma.j.cn501225-20250807-00351
K Chen, P W Lyu

Objective: To preliminarily evaluate the feasibility, safety, and early aesthetic outcomes of using the pectoralis major fascia in robotic nipple-sparing mastectomy with immediate breast reconstruction with gel implant (RNSMIBR). Methods: This study was a retrospective cohort study. From November 2023 to April 2025, 34 female breast cancer patients who met the inclusion criteria were treated at the First Affiliated Hospital of Zhengzhou University. Seventeen patients, aged 43±7 years, who underwent posterior subpectoral pocket expansion using a titanium mesh patch in RNSMIBR surgery were included into titanium mesh patch group, and 17 patients, aged 46±7 years, who underwent posterior subpectoral pocket expansion using the pectoralis major fascia in RNSMIBR surgery were included into pectoralis major fascia group. Surgery-related complications during postoperative hospitalization and follow-up within 30 days after discharge were recorded and graded using the Clavien-Dindo classification. During the follow-up of approximately 3 months postoperatively, the BREAST-Q scale was used to score the patients' satisfaction with breast appearance, psychosocial well-being, and satisfaction with medical care. Results: During postoperative hospitalization and follow-up within 30 days after discharge, three patients in titanium mesh patch group experienced grade Ⅰ complications. In pectoralis major fascia group, three patients experienced grade Ⅰ complications and three patients experienced grade Ⅲb complications. The grade Ⅲb complication in one patient was caused by radiotherapy. No complications occurred in other patients in the two groups. There was no statistically significant difference in the Clavien-Dindo classification of surgery-related complications between the two groups of patients (P>0.05). During the follow-up of approximately 3 months postoperatively, the BREAST-Q scale scores were 92±5 in titanium mesh patch group of patients and 79±28 in pectoralis major fascia group of patients, with no statistically significant difference between the two groups (P>0.05). Conclusions: The pectoralis major fascia-assisted expansion technique may serve as an alternative option of expansion in RNSMIBR when titanium mesh patch is unavailable or cost-prohibitive. For patients requiring postoperative radiotherapy, titanium mesh patch or other materials that do not rely on vascular supply are still preferred to balance aesthetic outcomes and complication control.

目的:初步评价胸大肌筋膜在机器人保留乳头乳房切除术即刻凝胶植入乳房重建术(RNSMIBR)中的可行性、安全性及早期美学效果。方法:本研究为回顾性队列研究。2023年11月至2025年4月,34例符合纳入标准的女性乳腺癌患者在郑州大学第一附属医院接受治疗。选取17例采用钛网补片进行RNSMIBR手术后胸下袋扩张的患者(年龄43±7岁)为钛网补片组,17例采用RNSMIBR手术后胸大肌筋膜进行胸大肌后袋扩张的患者(年龄46±7岁)为胸大肌筋膜组。记录术后住院期间及出院后30天内随访的手术相关并发症,并采用Clavien-Dindo分级法进行分级。在术后约3个月的随访中,采用breast - q量表对患者的乳房外观满意度、心理健康满意度和医疗服务满意度进行评分。结果:钛网补片组3例患者术后住院及出院后30天内随访期间出现Ⅰ级并发症。胸大肌筋膜组3例发生Ⅰ级并发症,3例发生Ⅲb级并发症。1例患者因放疗引起Ⅲb级并发症。两组其他患者均无并发症发生。两组患者手术相关并发症Clavien-Dindo分型比较,差异无统计学意义(P < 0.05)。在术后约3个月的随访中,胸大肌筋膜组和钛网贴片组的BREAST-Q评分分别为79±28分和92±5分,两组比较差异无统计学意义(P < 0.05)。结论:胸大肌筋膜辅助扩张技术可作为RNSMIBR患者在钛网补片不可用或成本过高时的另一种扩张方法。对于术后需要放疗的患者,仍然首选钛网补片或其他不依赖血管供应的材料,以平衡美观效果和并发症控制。
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引用次数: 0
[Clinical practice guideline for pediatric scar prevention and treatment (2025 edition)]. [小儿疤痕防治临床实践指南(2025年版)]。
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20250630-00285

Children constitute a key population for scar prevention and treatment, attributed to the unique features of their skin's physiological structure and psychosomatic growth. Current approaches for preventing and treating pediatric scars are formulated with reference to relevant consensuses and guidelines for adult, failing to fully consider the specific characteristics of pediatric scars and their special needs of growth and development. As a result, some strategies for prevention and treatment remain controversial. To address this limitation, the Chinese Burn Association brought together domestic and international experts in the field of scar prevention and treatment from relevant disciplines. Guided by evidence-based medicine, referring domestic and international literature, and combining the clinical experience of specialist physicians, the Clinical practice guideline for pediatric scar prevention and treatment (2025 edition) was finally developed after consultations on clinical issues and then multiple rounds of expert meetings for discussions. This guideline outlines 20 recom-mendations addressing 10 key issues in pediatric scar prevention and treatment, and discusses the controversial issues in this field, aiming to provide scientific guidance for the entire process of prevention, treatment, and rehabilitation of scars in children aged 1-14 years.

儿童由于其皮肤生理结构和身心发育的独特特征,构成了疤痕预防和治疗的关键人群。目前小儿疤痕的预防和治疗方法是参照成人的相关共识和指南制定的,没有充分考虑小儿疤痕的具体特点和生长发育的特殊需要。因此,一些预防和治疗策略仍然存在争议。为了解决这一问题,中国烧伤协会汇集了国内外相关学科瘢痕防治领域的专家。以循证医学为指导,参考国内外文献,结合专科医师的临床经验,经过对临床问题的协商,再经过多轮专家会议讨论,最终制定了《小儿瘢痕防治临床实践指南(2025年版)》。本指南针对儿童瘢痕防治的10个关键问题提出了20项建议,并对该领域存在的争议问题进行了探讨,旨在为1-14岁儿童瘢痕的预防、治疗和康复的全过程提供科学指导。
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引用次数: 0
[Practical guideline on the prevention and management of diabetic foot in China (Ⅰ)]. [中国糖尿病足防治实用指南(Ⅰ)]。
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20250801-00345

Diabetic foot is one of the common and serious complications among the patients with diabetes mellitus. The major causes of amputation and/or death in the patients are foot deformities, ulcers, ischemia, and possible concurrent infections. To further standardize diabetic foot prevention and treatment in China, improve its diagnostic and therapeutic consistency, and promote the development of a specialized tiered care system, Chinese Burn Association, Yangtze River Delta Integrated Diabetic Foot Alliance, and Editorial Committee of Chinese Journal of Burns and Wounds organized a multidisciplinary expert team. The team determined clinical issues related to the diagnosis, treatment, and prevention of diabetic foot and evaluated the quality grades of relevant evidence using the grading of recommendations assessment, development, and evaluation system, and eventually developed the Practical guideline on the prevention and management of diabetic foot in China. There were 46 recommendations formed in the guideline, covering comprehensive medical assessment, internal medical treatments such as control of blood glucose, blood pressure, and blood lipid, antithrombotic and anti-infection therapy, perioperative risk assessment and management, surgical treatments such as debridement, vascular reconstruction, and tissue repair surgeries, as well as foot disease prevention, multidisciplinary team cooperation, and the construction of a tiered care system, aiming to provide guidance for the clinical practice of diabetic foot in China.

糖尿病足是糖尿病患者常见且严重的并发症之一。患者截肢和/或死亡的主要原因是足部畸形、溃疡、缺血和可能的并发感染。为进一步规范我国糖尿病足的防治,提高诊断和治疗的一致性,促进专业化分级诊疗体系的发展,中华烧伤学会、长三角糖尿病足综合联盟、中国烧伤创面杂志编委会共同组织了多学科专家团队。该团队确定了与糖尿病足的诊断、治疗和预防相关的临床问题,并使用建议评估、开发和评估系统的分级来评估相关证据的质量等级,最终制定了中国糖尿病足预防和管理实用指南。《指南》共提出了46条建议,内容涉及综合医疗评估、血糖、血压、血脂控制等内科治疗、抗血栓、抗感染治疗、围手术期风险评估与管理、清创、血管重建、组织修复手术等外科治疗、足病预防、多学科团队合作、分层护理体系建设等。旨在为糖尿病足在中国的临床实践提供指导。
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引用次数: 0
[Application of multidisciplinary team diagnosis and treatment model in the management of patients with combined burns and heat stroke]. [多学科团队诊疗模式在烧伤合并中暑患者管理中的应用]。
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20250729-00330
S S Jiang, N N Wang, H H Wang, R Xiao, S L Zhang, C Wang, J Yan, D S Hu, S L Duan, Z C Lin, M Y Hu, S Li, M Wang, N Lyu, Y L Ji, Q Feng, Q H Li, G A Lin, T T Yan
<p><p><b>Objective:</b> To explore the application of multidisciplinary team (MDT) diagnosis and treatment model in the management of patients with combined burns and heat stroke. <b>Methods:</b> This study was a retrospective observational study. From January 2022 to December 2024, the Military Burn Center admitted 5 patients and the Department of Critical Care Medicine of the 990<sup>th</sup> Hospital of PLA Joint Logistic Support Force admitted 4 patients with combined burns and heat stroke that met the inclusion criteria. All patients were male, aged 64-78 (70±5) years. The onset of the disease in patients occurred mainly during June to September, with the time frame predominantly between 10:00 and 14:30, in environment with temperature>30 ℃ and humidity≥60%. Under the MDT diagnosis and treatment model, a fixed MDT was established with the director of the Military Burn Center as the leader, which included specialists from critical care medicine, nephrology, respiratory medicine, cardiology, neurology, anesthesiology, endocrinology, pharmacy, rehabilitation, nutrition, and transfusion medicine. Through clear division of responsibilities, standardized information communication, and daily interdisciplinary handovers, integrated coordination and decision-making in the diagnosis and treatment process were achieved. The burn-related characteristics including total burn area, burn index, and combination of inhalation injury, treatment including infusion rate in the first 24 h post admission, infusion rate in the second 24 h post admission, and total infusion rate within 48 h post admission, use of blood products, status of continuous blood purification (CBP) treatment, administration of invasive mechanical ventilation, and timing of the first surgery, outcomes including length of intensive care unit (ICU) stay, total hospital stay, and mortality within 7 days after admission of patients were recorded. <b>Results:</b> The total burn area of patients was 22.6% (10.5%, 23.0%) total body surface area, the burn index was 12.5 (8.0, 13.5), and 5 patients were combined with inhalation injury. The infusion rate in the first 24 h post admission, infusion rate in the second 24 h post admission, and total infusion rate within 48 h post admission of 7 patients were significantly higher than the planned fluid infusion rates (with <i>t</i> values of 4.39, 8.58, and 3.69, respectively, <i>P</i><0.05). Blood products were used in 6 patients. CBP was performed in 3 patients with an average duration of 64.7 h, and invasive mechanical ventilation was applied in 4 patients with an average duration of 60.0 h. Five patients underwent surgery, with the first surgery performed at 13-19 (16.0 (13.7, 19.0)) days post admission. The patients' length of ICU stay was 0-504 (216 (18, 252)) h, and the total hospital stay was 0.5-71.0 (11.0 (1.4, 46.5)) d. Four patients died within 7 days after admission. <b>Conclusions:</b> Patients with combined burns and heat stroke are charact
目的:探讨多学科团队(MDT)诊疗模式在烧伤合并中暑患者诊治中的应用。方法:本研究为回顾性观察研究。2022年1月至2024年12月,我军烧伤中心收治符合入选标准的烧伤合并中暑患者5例,联勤保障部队第990医院重症医学科收治符合入选标准的烧伤合并中暑患者4例。所有患者均为男性,年龄64 ~ 78(70±5)岁。患者发病时间以6 ~ 9月为主,发病时间以10:00 ~ 14:30为主,环境温度0 ~ 30℃,湿度≥60%。在MDT诊疗模式下,建立了以军事烧伤中心主任为牵头,由重症医学、肾内科、呼吸内科、心脏科、神经内科、麻醉科、内分泌科、药剂科、康复科、营养科、输血医学等专家组成的固定MDT。通过明确的职责分工、规范的信息沟通和日常的跨学科交接,实现了诊疗过程中的综合协调和决策。烧伤相关特征包括总烧伤面积、烧伤指数、吸入性损伤的合并,治疗包括入院后第24小时的输液率、入院后第24小时的输液率、入院后48小时内的总输液率,血液制品的使用情况、持续血液净化(CBP)治疗情况、有无有创机械通气、首次手术时间等。结果包括重症监护病房(ICU)住院时间、总住院时间和患者入院后7天内的死亡率。结果:患者烧伤总面积为体表总面积的22.6%(10.5%,23.0%),烧伤指数为12.5(8.0,13.5),合并吸入性损伤5例。7例患者入院后前24 h的输液速率、入院后后24 h的输液速率、入院后48 h内的总输液速率均显著高于计划输液速率(t值分别为4.39、8.58、3.69)。烧伤合并中暑患者烧伤面积相对有限、吸入性损伤频繁、液体复苏量大,需要多种器官支持方式、手术时机延迟等临床挑战。在此背景下,以烧伤科和重症医学科为中心的MDT诊疗模式为协调复杂的复苏管理、器官功能支持和手术时机决策提供了合理的途径。
{"title":"[Application of multidisciplinary team diagnosis and treatment model in the management of patients with combined burns and heat stroke].","authors":"S S Jiang, N N Wang, H H Wang, R Xiao, S L Zhang, C Wang, J Yan, D S Hu, S L Duan, Z C Lin, M Y Hu, S Li, M Wang, N Lyu, Y L Ji, Q Feng, Q H Li, G A Lin, T T Yan","doi":"10.3760/cma.j.cn501225-20250729-00330","DOIUrl":"10.3760/cma.j.cn501225-20250729-00330","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To explore the application of multidisciplinary team (MDT) diagnosis and treatment model in the management of patients with combined burns and heat stroke. &lt;b&gt;Methods:&lt;/b&gt; This study was a retrospective observational study. From January 2022 to December 2024, the Military Burn Center admitted 5 patients and the Department of Critical Care Medicine of the 990&lt;sup&gt;th&lt;/sup&gt; Hospital of PLA Joint Logistic Support Force admitted 4 patients with combined burns and heat stroke that met the inclusion criteria. All patients were male, aged 64-78 (70±5) years. The onset of the disease in patients occurred mainly during June to September, with the time frame predominantly between 10:00 and 14:30, in environment with temperature&gt;30 ℃ and humidity≥60%. Under the MDT diagnosis and treatment model, a fixed MDT was established with the director of the Military Burn Center as the leader, which included specialists from critical care medicine, nephrology, respiratory medicine, cardiology, neurology, anesthesiology, endocrinology, pharmacy, rehabilitation, nutrition, and transfusion medicine. Through clear division of responsibilities, standardized information communication, and daily interdisciplinary handovers, integrated coordination and decision-making in the diagnosis and treatment process were achieved. The burn-related characteristics including total burn area, burn index, and combination of inhalation injury, treatment including infusion rate in the first 24 h post admission, infusion rate in the second 24 h post admission, and total infusion rate within 48 h post admission, use of blood products, status of continuous blood purification (CBP) treatment, administration of invasive mechanical ventilation, and timing of the first surgery, outcomes including length of intensive care unit (ICU) stay, total hospital stay, and mortality within 7 days after admission of patients were recorded. &lt;b&gt;Results:&lt;/b&gt; The total burn area of patients was 22.6% (10.5%, 23.0%) total body surface area, the burn index was 12.5 (8.0, 13.5), and 5 patients were combined with inhalation injury. The infusion rate in the first 24 h post admission, infusion rate in the second 24 h post admission, and total infusion rate within 48 h post admission of 7 patients were significantly higher than the planned fluid infusion rates (with &lt;i&gt;t&lt;/i&gt; values of 4.39, 8.58, and 3.69, respectively, &lt;i&gt;P&lt;/i&gt;&lt;0.05). Blood products were used in 6 patients. CBP was performed in 3 patients with an average duration of 64.7 h, and invasive mechanical ventilation was applied in 4 patients with an average duration of 60.0 h. Five patients underwent surgery, with the first surgery performed at 13-19 (16.0 (13.7, 19.0)) days post admission. The patients' length of ICU stay was 0-504 (216 (18, 252)) h, and the total hospital stay was 0.5-71.0 (11.0 (1.4, 46.5)) d. Four patients died within 7 days after admission. &lt;b&gt;Conclusions:&lt;/b&gt; Patients with combined burns and heat stroke are charact","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"41 11","pages":"1083-1090"},"PeriodicalIF":0.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12672199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145656611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Analyzing the multidisciplinary strategies for diagnosis and treatment of patients with burns and trauma from a management perspective]. [从管理角度分析烧伤创伤患者的多学科诊断和治疗策略]。
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20250731-00341
H Chen

Patients with burns and trauma often have complex conditions that require multidisciplinary diagnosis and treatment as a single discipline is insufficient to meet the needs of modern medical goals. Multidisciplinary team (MDT) collaborative diagnosis and treatment, as an important model for solving this problem, faces challenges such as the lack of initiation mechanisms, loose process management, and absence of value-based incentives in practical clinical applications. From a management perspective, this article analyzes the essential differences between MDT collaborative diagnosis and treatment for burn and trauma patients and traditional consultations, and explores the core issues from the technical level to management innovation, including leveraging on the advance technologies of burn discipline, relying on the strong disciplines of medical institutions, integrating disciplines centered on patients, optimizing processes with management as a link, and driving long-term operation with performance-based incentives. The aim is to provide strategic references for the effective implementation of MDT collaborative diagnosis and treatment for burn and trauma patients.

烧伤和创伤患者往往病情复杂,需要多学科的诊断和治疗,因为单一学科不足以满足现代医学目标的需要。多学科团队(MDT)协同诊疗作为解决这一问题的重要模式,在实际临床应用中面临着启动机制缺乏、流程管理松散、缺乏基于价值的激励等挑战。本文从管理角度分析烧伤创伤患者MDT协同诊疗与传统会诊的本质区别,探讨从技术层面到管理创新的核心问题,包括利用烧伤学科的先进技术、依托医疗机构的强学科、以患者为中心的学科整合、以管理为纽带的流程优化等。并以绩效激励推动长期运营。旨在为烧伤创伤患者有效实施MDT协同诊疗提供战略参考。
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引用次数: 0
[Multidisciplinary integration in the diagnosis and treatment in burns and wound repair surgery: current status, challenges, and future]. [烧伤创面修复手术诊断与治疗的多学科融合:现状、挑战与未来]。
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20250901-00379
H Guan, D L Zhang

Burn medicine, as the only clinical discipline named based on etiology, is an interdisciplinary subject that integrates burn trauma emergency, wound repair, functional reconstruction, and comprehensive rehabilitation. The history of burn medicine development is an evolutionary history of the continuous integration and permeation of multi-disciplinary technologies and the continuous improvement of theoretical systems. In recent years, with the improvement of public protection awareness and changes in the disease spectrum, the burn discipline is undergoing a profound transformation from single-specialty treatment to the multidisciplinary team (MDT) collaborative diagnosis and treatment model. Its scope has gradually expanded to a more integrated burns and wound repair surgery. This article starts from the changes in the disease spectrum of the discipline, systematically analyzes the necessity, current status, and main practice models of multidisciplinary integration in the diagnosis and treatment of burns and wound repair surgery. It explores the opportunities and challenges that cutting-edge technologies such as artificial intelligence and regenerative medicine pose to the innovative development of the discipline in promoting the MDT collaboration, and puts forward the future direction for the development of MDT collaborative diagnosis and treatment in burns and wound repair surgery, hoping to provide a reference for the discipline construction.

烧伤医学是临床唯一一门以病因命名的学科,是集烧伤急诊、创面修复、功能重建、综合康复为一体的交叉学科。烧伤医学发展史就是一部多学科技术不断融合和渗透、理论体系不断完善的进化史。近年来,随着公众保护意识的提高和疾病谱的变化,烧伤学科正经历着从单一专科治疗向多学科团队(MDT)协同诊疗模式的深刻转变。它的范围逐渐扩大到更综合的烧伤和伤口修复手术。本文从学科疾病谱的变化出发,系统分析了烧伤创面修复外科多学科融合诊疗的必要性、现状及主要实践模式。探讨人工智能、再生医学等前沿技术在推动MDT协同发展中对学科创新发展的机遇与挑战,并为烧伤创面修复手术MDT协同诊疗的发展提出未来方向,希望为学科建设提供参考。
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引用次数: 0
[Clinical application effects of indocyanine green angiography combined with color Doppler ultrasound in perforator localization of anterolateral thigh perforator flaps]. 【吲哚菁绿血管造影联合彩色多普勒超声在股前外侧穿支皮瓣穿支定位中的临床应用效果】。
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20240629-00254
S Dong, S Wang, Z C Teng, Y C Liu, Y Zhang, Y Cao, K Wang, R Zhou, G Z Jin, J H Ju
<p><p><b>Objective:</b> To investigate the clinical application effects of indocyanine green angiography (ICGA) combined with color Doppler ultrasound (CDU) in perforator localization of anterolateral thigh perforator flaps. <b>Methods:</b> This study was a retrospective observational study. From January to August 2024, the Department of Hand Surgery admitted 21 patients and the Department of Wound Repair Surgery of Suzhou Ruihua Orthopedic Hospital admitted 35 patients that met the inclusion criteria. These patients underwent surgical repair of the extremity skin and soft tissue defects using anterolateral thigh perforator flaps designed under the assistance of CDU and ICGA. Among them, there were 44 males and 12 females, aged 16 to 71 years. After debridement, the wound area ranged from 7.0 cm×5.5 cm to 40.0 cm×10.0 cm. Before surgery, CDU and ICGA were used to locate the perforators of anterolateral thigh perforator flap. The area of harvested flap ranged from 8.0 cm×6.0 cm to 40.5 cm×11.0 cm. The wounds in flap donor sites were closed directly or covered with the full-thickness skin graft from the contralateral thigh. The number of perforators located by preoperative CDU or ICGA, and the number, origin, type, and caliber of perforators in intraoperative exploration, and flap thickness were recorded. The consistency between preoperative CDU or ICGA localization results and intraoperative exploration findings was assessed. The error distances between preoperative CDU or ICGA localization and intraoperative exploration of perforator, respectively, were measured. Using intraoperative exploration of perforator as the gold standard, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of preoperative CDU and ICGA for perforator localization were calculated. The independent influencing factor for the consistency between CDU or ICGA localization results and intraoperative exploration findings of 56 patients who had anterolateral thigh perforator flap designed under the assistance of CDU and ICGA were screened. <b>Results:</b> A total of 131 perforators were located by CDU and 130 perforators were located by ICGA preoperatively. Intraoperatively, 132 perforators were explored, including 64 perforators from the oblique branch, 49 perforators from the descending branch, 9 perforators from the transverse branch, and 10 perforators from the anterior branch. Among them, 46 perforators were septocutaneous perforators and 86 perforators were musculocutaneous perforators, with a mean caliber of (0.72±0.21) mm. The flap thickness was (1.5±0.6) cm. There was no statistically significant difference in the consistency between preoperative CDU localization results and intraoperative exploration findings and ICGA localization results and intraoperative exploration findings (<i>P</i>>0.05). There was no statistically significant difference in the error distance between preoperative CDU localization and intraoperative explo
目的:探讨吲哚菁绿血管造影(ICGA)联合彩色多普勒超声(CDU)在股前外侧穿支皮瓣穿支定位中的临床应用效果。方法:本研究为回顾性观察研究。2024年1 - 8月,苏州瑞华骨科医院手外科收治符合纳入标准的患者21例,伤口修复外科收治符合纳入标准的患者35例。这些患者采用在CDU和ICGA辅助下设计的大腿前外侧穿支皮瓣修复四肢皮肤和软组织缺损。其中男44人,女12人,年龄16至71岁。清创后创面面积7.0 cm×5.5 ~ 40.0 cm×10.0 cm。术前应用CDU和ICGA定位股前外侧穿支皮瓣穿支。皮瓣切除面积为8.0 cm×6.0 ~ 40.5 cm×11.0 cm。皮瓣供区创面直接封闭或用对侧大腿全层皮片覆盖。记录术前CDU或ICGA定位的穿支数量,术中探查穿支的数量、来源、类型、口径及皮瓣厚度。评估术前CDU或ICGA定位结果与术中探查结果的一致性。分别测量术前CDU或ICGA定位与术中探查穿支的误差距离。以术中探查穿支为金标准,计算术前CDU和ICGA对穿支定位的敏感性、特异性、阳性预测值、阴性预测值和准确性。筛选56例在CDU和ICGA辅助下设计大腿前外侧穿支皮瓣的患者CDU或ICGA定位结果与术中探查结果一致性的独立影响因素。结果:术前CDU定位131支穿支,ICGA定位130支。术中共探查了132个穿支,其中斜支64个,降支49个,横支9个,前支10个。其中隔皮穿支46支,肌皮穿支86支,平均口径为(0.72±0.21)mm,皮瓣厚度为(1.5±0.6)cm,术前CDU定位结果与术中探查结果、ICGA定位结果与术中探查结果的一致性比较,差异均无统计学意义(P < 0.05)。术前CDU定位术中探查穿支与术前ICGA定位术中探查穿支的误差距离差异无统计学意义(P < 0.05)。ICGA和CDU定位穿支的敏感性、特异性、阳性预测值、阴性预测值和准确性分别为92.42%、92.00%、93.85%、90.20%和92.24%、84.09%、80.00%、84.73%、79.21%和82.33%。56例在CDU和ICGA辅助下设计股前外侧穿支皮瓣的患者,皮瓣厚度是影响术前ICGA定位结果与术中探查结果一致性的因素(优势比为0.20,95%可信区间为0.06 ~ 0.66,ppppp)。临床应用ICGA与CDU联合应用于股前外侧穿支皮瓣穿支定位,可相互补充,提高定位精度。皮瓣厚度显著影响ICGA定位精度,穿支类型显著影响CDU定位精度。
{"title":"[Clinical application effects of indocyanine green angiography combined with color Doppler ultrasound in perforator localization of anterolateral thigh perforator flaps].","authors":"S Dong, S Wang, Z C Teng, Y C Liu, Y Zhang, Y Cao, K Wang, R Zhou, G Z Jin, J H Ju","doi":"10.3760/cma.j.cn501225-20240629-00254","DOIUrl":"10.3760/cma.j.cn501225-20240629-00254","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the clinical application effects of indocyanine green angiography (ICGA) combined with color Doppler ultrasound (CDU) in perforator localization of anterolateral thigh perforator flaps. &lt;b&gt;Methods:&lt;/b&gt; This study was a retrospective observational study. From January to August 2024, the Department of Hand Surgery admitted 21 patients and the Department of Wound Repair Surgery of Suzhou Ruihua Orthopedic Hospital admitted 35 patients that met the inclusion criteria. These patients underwent surgical repair of the extremity skin and soft tissue defects using anterolateral thigh perforator flaps designed under the assistance of CDU and ICGA. Among them, there were 44 males and 12 females, aged 16 to 71 years. After debridement, the wound area ranged from 7.0 cm×5.5 cm to 40.0 cm×10.0 cm. Before surgery, CDU and ICGA were used to locate the perforators of anterolateral thigh perforator flap. The area of harvested flap ranged from 8.0 cm×6.0 cm to 40.5 cm×11.0 cm. The wounds in flap donor sites were closed directly or covered with the full-thickness skin graft from the contralateral thigh. The number of perforators located by preoperative CDU or ICGA, and the number, origin, type, and caliber of perforators in intraoperative exploration, and flap thickness were recorded. The consistency between preoperative CDU or ICGA localization results and intraoperative exploration findings was assessed. The error distances between preoperative CDU or ICGA localization and intraoperative exploration of perforator, respectively, were measured. Using intraoperative exploration of perforator as the gold standard, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of preoperative CDU and ICGA for perforator localization were calculated. The independent influencing factor for the consistency between CDU or ICGA localization results and intraoperative exploration findings of 56 patients who had anterolateral thigh perforator flap designed under the assistance of CDU and ICGA were screened. &lt;b&gt;Results:&lt;/b&gt; A total of 131 perforators were located by CDU and 130 perforators were located by ICGA preoperatively. Intraoperatively, 132 perforators were explored, including 64 perforators from the oblique branch, 49 perforators from the descending branch, 9 perforators from the transverse branch, and 10 perforators from the anterior branch. Among them, 46 perforators were septocutaneous perforators and 86 perforators were musculocutaneous perforators, with a mean caliber of (0.72±0.21) mm. The flap thickness was (1.5±0.6) cm. There was no statistically significant difference in the consistency between preoperative CDU localization results and intraoperative exploration findings and ICGA localization results and intraoperative exploration findings (&lt;i&gt;P&lt;/i&gt;&gt;0.05). There was no statistically significant difference in the error distance between preoperative CDU localization and intraoperative explo","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"41 11","pages":"1091-1100"},"PeriodicalIF":0.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12672202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145656614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Effect and mechanism of astaxanthin on the aging of high glucose-treated human skin fibroblasts]. 虾青素对高糖处理人皮肤成纤维细胞衰老的影响及机制
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20250109-00018
L J Tang, L H Fan, J R Wang, M Luo, X X Yang, R Q Zhong, H Y Gao
<p><p><b>Objective:</b> To investigate the effect and mechanism of astaxanthin on the aging of high glucose-treated human skin fibroblasts (Fbs). <b>Methods:</b> The study was an experimental study. Human skin Fbs were collected and divided into control group with conventional culture, high glucose group treated with glucose at a final molarity of 30 mmol/L, and low astaxanthin group and high astaxanthin group pretreated with astaxanthin at final molarities of 25 and 50 μmol/L respectively and then treated as that in high glucose group. After 48 h of culture, the cell survival rate was detected by the cell counting kit-8, the reactive oxygen species level in cells was detected by the fluorescent probe method, the levels of malondialdehyde, glutathione, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in cells were detected by colorimetry, and the protein expression levels of p53, phosphorylated p53, p21, p16, Rb, phosphorylated Rb, matrix metalloproteinase 1 (MMP1), MMP3, and MMP13 in cells and nuclear factor-erythroid 2-related factor 2 (Nrf2) in cytoplasm and nucleus were detected by Western blotting. Another batch of human skin Fbs were collected and divided into control group, high glucose group, and high astaxanthin group which were treated as before, and high astaxanthin+ML385 group, which were pretreated with astaxanthin at a final molarity of 50 μmol/L and ML385 at a final molarity of 10 μmol/L respectively and then treated as that in high glucose group. After 48 h of culture, the cell survival rate, the protein expression levels of p53, phosphorylated p53, p21, p16, Rb, phosphorylated Rb, MMP1, MMP3, and MMP13 in cells and Nrf2 in cytoplasm and nucleus were detected as before. The sample number in all experiments above was 3. <b>Results:</b> After 48 h of culture, compared with that in control group, the cell survival rate in high glucose group was significantly decreased (<i>P</i><0.05); compared with that in high glucose group, the cell survival rates in low astaxanthin group and high astaxanthin group were significantly increased (<i>P</i><0.05); compared with that in low astaxanthin group, the cell survival rate in high astaxanthin group was significantly increased (<i>P</i><0.05). Compared with those in control group, the levels of reactive oxygen species and malondialdehyde in cells were significantly increased (<i>P</i><0.05), while the levels of glutathione, SOD, CAT, and GSH-Px in cells were significantly decreased in high glucose group (<i>P</i><0.05). Compared with those in high glucose group, the levels of reactive oxygen species and malondialdehyde in cells were significantly decreased (<i>P</i><0.05), while the levels of CAT and GSH-Px in cells were significantly increased in low astaxanthin group (<i>P</i><0.05); the levels of reactive oxygen species and malondialdehyde in cells were significantly decreased (<i>P</i><0.05), while the levels of glutathione, SOD, CAT, and GSH-Px in cells were s
目的:探讨虾青素对高糖处理的人皮肤成纤维细胞衰老的影响及其机制。方法:本研究为实验研究。采集人皮肤Fbs分为常规培养对照组,高糖组用终摩尔浓度为30 mmol/L的葡萄糖处理,低虾青素组和高虾青素组分别用终摩尔浓度为25 μmol/L和50 μmol/L的虾青素预处理,再按高糖组处理。培养48 h后,采用细胞计数试剂盒-8检测细胞存活率,采用荧光探针法检测细胞内活性氧水平,采用比色法检测细胞内丙二醛、谷胱甘肽、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)水平,检测细胞内p53、磷酸化p53、p21、p16、Rb、磷酸化Rb、基质金属蛋白酶1 (MMP1)、MMP3、Western blotting检测细胞中MMP13及细胞质和细胞核中核因子-红细胞2相关因子2 (Nrf2)的表达。另取一批人皮肤Fbs,分为对照组、高糖组和高虾青素组,按原方法处理;高虾青素+ML385组,分别用终摩尔浓度为50 μmol/L的虾青素和终摩尔浓度为10 μmol/L的ML385预处理,再按高糖处理。培养48 h后,检测细胞存活率,细胞内p53、磷酸化p53、p21、p16、Rb、磷酸化Rb、MMP1、MMP3、MMP13蛋白表达水平,细胞质和细胞核内Nrf2蛋白表达水平。以上所有实验的样本数均为3。结果:培养48 h后,与对照组相比,高糖组细胞存活率显著降低(ppppppppppppppppp值均为P值均为pppppppppp值均为PPPPPPPP值均为ppppppppppp值均为ppppppppp值均为ppppppppp值均为ppppppppp值均为ppppppppp值均为ppppppppp值均为ppppppppp值均为ppppppppp值均为ppppppppp值均为ppppppp值)结论:虾青素可通过调节Nrf2核转位抑制氧化应激,下调衰老相关蛋白的表达来缓解高糖处理的人皮肤Fbs的衰老。
{"title":"[Effect and mechanism of astaxanthin on the aging of high glucose-treated human skin fibroblasts].","authors":"L J Tang, L H Fan, J R Wang, M Luo, X X Yang, R Q Zhong, H Y Gao","doi":"10.3760/cma.j.cn501225-20250109-00018","DOIUrl":"10.3760/cma.j.cn501225-20250109-00018","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the effect and mechanism of astaxanthin on the aging of high glucose-treated human skin fibroblasts (Fbs). &lt;b&gt;Methods:&lt;/b&gt; The study was an experimental study. Human skin Fbs were collected and divided into control group with conventional culture, high glucose group treated with glucose at a final molarity of 30 mmol/L, and low astaxanthin group and high astaxanthin group pretreated with astaxanthin at final molarities of 25 and 50 μmol/L respectively and then treated as that in high glucose group. After 48 h of culture, the cell survival rate was detected by the cell counting kit-8, the reactive oxygen species level in cells was detected by the fluorescent probe method, the levels of malondialdehyde, glutathione, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in cells were detected by colorimetry, and the protein expression levels of p53, phosphorylated p53, p21, p16, Rb, phosphorylated Rb, matrix metalloproteinase 1 (MMP1), MMP3, and MMP13 in cells and nuclear factor-erythroid 2-related factor 2 (Nrf2) in cytoplasm and nucleus were detected by Western blotting. Another batch of human skin Fbs were collected and divided into control group, high glucose group, and high astaxanthin group which were treated as before, and high astaxanthin+ML385 group, which were pretreated with astaxanthin at a final molarity of 50 μmol/L and ML385 at a final molarity of 10 μmol/L respectively and then treated as that in high glucose group. After 48 h of culture, the cell survival rate, the protein expression levels of p53, phosphorylated p53, p21, p16, Rb, phosphorylated Rb, MMP1, MMP3, and MMP13 in cells and Nrf2 in cytoplasm and nucleus were detected as before. The sample number in all experiments above was 3. &lt;b&gt;Results:&lt;/b&gt; After 48 h of culture, compared with that in control group, the cell survival rate in high glucose group was significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05); compared with that in high glucose group, the cell survival rates in low astaxanthin group and high astaxanthin group were significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05); compared with that in low astaxanthin group, the cell survival rate in high astaxanthin group was significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with those in control group, the levels of reactive oxygen species and malondialdehyde in cells were significantly increased (&lt;i&gt;P&lt;/i&gt;&lt;0.05), while the levels of glutathione, SOD, CAT, and GSH-Px in cells were significantly decreased in high glucose group (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Compared with those in high glucose group, the levels of reactive oxygen species and malondialdehyde in cells were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05), while the levels of CAT and GSH-Px in cells were significantly increased in low astaxanthin group (&lt;i&gt;P&lt;/i&gt;&lt;0.05); the levels of reactive oxygen species and malondialdehyde in cells were significantly decreased (&lt;i&gt;P&lt;/i&gt;&lt;0.05), while the levels of glutathione, SOD, CAT, and GSH-Px in cells were s","PeriodicalId":516861,"journal":{"name":"Zhonghua shao shang yu chuang mian xiu fu za zhi","volume":"41 11","pages":"1101-1110"},"PeriodicalIF":0.0,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12672200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145656679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Indicative effect of indocyanine green-near-infrared fluorescence imaging in the thickness of necrotic dermal tissue in porcine burn wounds]. [吲哚菁绿-近红外荧光成像对猪烧伤创面坏死真皮组织厚度的指示作用]。
Pub Date : 2025-11-20 DOI: 10.3760/cma.j.cn501225-20250731-00337
Y S Zhang, X Pan, Y L Ge, J C Yue, Y X Song, W Y Song, R Zhao
<p><p><b>Objective:</b> To explore the indicative effects of indocyanine green-near-infrared fluorescence imaging (ICG-NIFI) in the thickness of necrotic dermal tissue in porcine burn wounds. <b>Methods:</b> This study was a medical-engineering interdisciplinary basic research. One 3-month-old male Bama miniature pig was selected, and circular burn wounds with a diameter of 2 cm were created by applying a scalding instrument with inflicting temperature of 75 ℃ to the skin on the dorsal side of the pig for 3, 7, and 11 seconds, respectively, with two wounds for each duration of injury. The fluorescence intensity of the regions of interest in the wounds and the surrounding normal skin was detected by ICG-NIFI technology within 700 seconds after injecting indocyanine green (hereinafter referred to as after administration). The trend of the normalized fluorescence intensity was observed, and the time when the fluorescence intensity of the regions of interest in the wounds and the surrounding normal skin reached the peak (hereinafter referred to as the peak time) was determined. Additional two 3-month-old male Bama miniature pigs were selected, and circular burn wounds with a diameter of 2 cm were created on the skin of bilateral thoracic walls by applying a scalding instrument with inflicting temperature of 75 ℃ for 5, 7, 9, 11, 13, 15, 17, and 19 seconds, respectively, with 4 wounds for each duration of injury. The blood flow intensity of the regions of interest in the wounds was detected by laser speckle contrast imaging technology; the relative fluorescence intensity of the regions of interest in the wounds was detected by ICG-NIFI technology at the peak time of the wounds and the surrounding normal skin. The full-thickness skin tissue of the wounds was taken for hematoxylin-eosin staining, and the thickness of necrotic dermal tissue was measured. The correlation between the blood flow intensity of the regions of interest in the wounds and the relative fluorescence intensity at the peak time of the regions of interest in the wounds and the surrounding normal skin and the thickness of necrotic dermal tissue in the wounds was analyzed. <b>Results:</b> The fluorescence intensity of the regions of interest in the surrounding normal skin showed a rapid increase followed by a slow decrease, with the peak time of approximately 60 seconds after administration. The fluorescence intensity of the regions of interest in the wounds showed a slow increase followed by a sustained stable trend, with the peak time of approximately 600 seconds after administration. The thickness of necrotic dermal tissue in the wounds caused by durations of injury of 5, 7, 9, 11, 13, 15, 17, and 19 seconds was (101±8), (130±6), (201±19), (197±30), (204±21), (280±39), (302±35), and (366±27) μm, respectively. The correlation between the blood flow intensity of the regions of interest in the wounds and the thickness of necrotic dermal tissue in the wounds was not significant (<i>P</i>
目的:探讨吲哚菁绿近红外荧光成像(ICG-NIFI)对猪烧伤创面真皮坏死组织厚度的指示作用。方法:本研究为医学工程交叉学科基础研究。选择1头3月龄雄性巴马小型猪,用75℃的烫伤器具分别在猪背部皮肤上烫伤3、7、11秒,形成直径为2 cm的圆形烧伤创面,每个烫伤持续时间为2个创面。注射吲哚菁绿后(以下简称给药后)700秒内,采用ICG-NIFI技术检测创面及周围正常皮肤感兴趣区域的荧光强度。观察归一化荧光强度的变化趋势,确定创面及周围正常皮肤感兴趣区域荧光强度达到峰值的时间(以下简称峰值时间)。另外选择2头3月龄雄性巴马小型猪,在双侧胸壁皮肤上分别施加75℃烫伤5、7、9、11、13、15、17、19秒,直径为2 cm的圆形烧伤创面,每个烫伤持续时间4个创面。采用激光散斑对比成像技术检测创面感兴趣区域血流强度;采用ICG-NIFI技术在创面及周围正常皮肤的峰值时间检测创面感兴趣区域的相对荧光强度。取创面全层皮肤组织进行苏木精-伊红染色,测定坏死真皮组织厚度。分析创面感兴趣区域血流强度与创面及周围正常皮肤感兴趣区域峰值时相对荧光强度与创面坏死真皮组织厚度的相关性。结果:正常皮肤周围感兴趣区域的荧光强度呈先上升后缓慢下降的趋势,峰值时间约为给药后60秒。伤口感兴趣区域的荧光强度表现出缓慢增加,随后呈持续稳定的趋势,峰值时间约为给药后600秒。损伤时间为5、7、9、11、13、15、17、19秒时,创面真皮坏死组织厚度分别为(101±8)、(130±6)、(201±19)、(197±30)、(204±21)、(280±39)、(302±35)、(366±27)μm。创面感兴趣区域血流强度与创面坏死真皮组织厚度的相关性无统计学意义(P < 0.05)。给药后60秒创面感兴趣区域的相对荧光强度与创面坏死真皮组织厚度呈显著负相关(R²=0.97,PR²=0.96,p)。ig - nifi在给药后60秒和600秒检测到猪烧伤创面感兴趣区域的相对荧光强度与创面坏死真皮组织厚度显著相关,说明ig - nifi对猪烧伤创面坏死真皮组织厚度具有显著敏感的指示作用。
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Zhonghua shao shang yu chuang mian xiu fu za zhi
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