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An Assessment of the Effects of Guanidinoacetic Acid on the Performance and Immune Response of Laying Hens Fed Diets with Three Levels of Metabolizable Energy 评估胍基乙酸对饲喂三种代谢能水平日粮的蛋鸡生产性能和免疫反应的影响
Pub Date : 2024-06-01 DOI: 10.3390/ani14111675
Santiago García-Gómora, G. Gómez-Verduzco, C. Márquez-Mota, A. Cortés-Cuevas, O. Vazquez-Mendoza, Ernesto Ávila-González
Simple Summary Annually, the demand for food to supply the world’s population is increasing, and the demand for poultry products is constantly increasing. Therefore, to meet these requirements, it is necessary to implement low-cost poultry diets. One way to reduce the cost of production is with the use of feed additives that increase the amount of metabolizable energy, such as guanidinoacetic acid (GAA). In the present study, the effect of GAA in diets with three levels of metabolizable energy (ME) was evaluated. The addition of GAA to low-ME diets maintained egg production and egg mass at similar levels to those of hens fed a high-ME diet, which indicates that adding GAA is a promising nutritional strategy for reducing the level of ME in the diets of laying hens. Abstract Different levels of metabolizable energy (ME) and the inclusion of guanidinoacetic acid (GAA) in the diet of 53-week-old Lohmann LSL-CLASSIC hens were used to evaluate its effect on reproductive parameters, egg quality, intestinal morphology, and the immune response. Six diets were used in a 3 × 2 factorial design, with three levels of ME (2850, 2800, and 2750 kcal/kg), and with (0.08%) or without the inclusion of GAA. The addition of GAA to diets with low levels of ME increased (p < 0.05) egg production and egg mass. Moreover, hens fed with 2800 kcal/g without GAA had the highest concentration (p < 0.05) of serum interleukin IL-2, while those fed diets with the same amount of ME but supplemented with 0.08% GAA had the lowest concentration. Finally, the inclusion of 0.08% GAA increased (p < 0.05) the concentration of vascular endothelial growth factor (VEGF), regardless of the ME level in the diet. This study highlights the potential role of GAA in decreasing the energy level of ME (50–100 kcal/g) in the feeding of hens and in the modulation of specific immune responses. Further research is recommended to fully understand the mechanisms of action of GAA on the mechanism target of rapamycin and its relationship with the immune response.
简单摘要 每年,世界人口对粮食的需求不断增加,对家禽产品的需求也在不断增加。因此,要满足这些需求,就必须采用低成本的家禽日粮。降低生产成本的方法之一是使用能增加代谢能的饲料添加剂,如鸟苷酸(GAA)。本研究评估了 GAA 在三种代谢能(ME)水平日粮中的效果。在低代谢能日粮中添加 GAA 可使蛋鸡的产蛋量和蛋重保持在与饲喂高代谢能日粮相似的水平,这表明添加 GAA 是降低蛋鸡日粮中代谢能水平的一种可行的营养策略。摘要 在53周龄的Lohmann LSL-CLASSIC蛋鸡日粮中添加不同水平的代谢能(ME)和鸟苷酸(GAA),以评估其对繁殖参数、鸡蛋质量、肠道形态和免疫反应的影响。在 3 × 2 的因子设计中使用了六种日粮,三种 ME 水平(2850、2800 和 2750 千卡/千克),添加(0.08%)或不添加 GAA。在低水平 ME 的日粮中添加 GAA 可提高产蛋量和蛋重(p < 0.05)。此外,饲喂 2800 千卡/克而不添加 GAA 的母鸡血清白细胞介素 IL-2 的浓度最高(p < 0.05),而饲喂相同 ME 但添加 0.08% GAA 的日粮的母鸡血清白细胞介素 IL-2 的浓度最低。最后,无论日粮中的 ME 水平如何,添加 0.08% GAA 都会增加血管内皮生长因子(VEGF)的浓度(p < 0.05)。这项研究强调了 GAA 在降低母鸡饲粮中 ME 的能量水平(50-100 千卡/克)和调节特定免疫反应方面的潜在作用。建议开展进一步研究,以充分了解 GAA 对雷帕霉素机制靶点的作用机制及其与免疫反应的关系。
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引用次数: 0
Effects of Acetic Acid and Morphine in Shore Crabs, Carcinus maenas: Implications for the Possibility of Pain in Decapods 醋酸和吗啡对梭子蟹的影响:对无脊椎动物疼痛可能性的影响
Pub Date : 2024-06-01 DOI: 10.3390/ani14111705
Stuart Barr, Robert W Elwood
Simple Summary Injection of acetic acid into one leg of a crab caused rubbing of that leg and holding the leg off the floor of a tank. Such activities directed towards the site of a noxious stimulus are consistent with the idea of pain. Some crabs also cast off the leg injected with acid. Because that occurred in association with possible pain-related behaviour, it too might be caused by pain. Prior morphine injection caused various behavioural changes, but morphine did not ameliorate the responses to acetic acid. Therefore, morphine does not act as an analgesic, and this agrees with previous work. Nevertheless, the directed behaviour that follows injection into a leg agrees with other studies and provides additional evidence suggesting that these animals experience pain. Abstract Noxious chemicals, coupled with morphine treatment, are often used in studies on pain in vertebrates. Here we show that injection of morphine caused several behavioural changes in the crab, Carcinus maenas, including reduced pressing against the sides of the enclosure and more rubbing and picking at the mouth parts and, at least for a short time, more defensive displays. Subsequent injection of acetic acid into one rear leg caused rubbing of the injected leg and the injected leg was held vertically off the ground. These activities directed at or involving the specific leg are consistent with previous observations of directed behaviour following noxious stimuli and are consistent with the idea that decapods experience pain. Further, acetic acid but not injection of water induced autotomy of the injected leg in these animals. Because autotomy is temporally associated with directed behaviour, it is possible that the autotomy is a pain-related response. Acetic acid is clearly a noxious substance when applied to decapods. However, morphine had no effect on the activities associated with acetic acid injection and thus there is no evidence for an analgesic effect. Further, the injection of acetic acid did not interfere with behavioural effects of morphine. The activities directed towards the site of injection are like those observed with injection, or with external application, of various noxious substances and the present study adds to a growing body of knowledge about possible pain in decapods.
简单摘要 向螃蟹的一条腿注射醋酸会引起该腿的摩擦,并使该腿离开水箱的地面。这种针对有害刺激部位的活动符合疼痛的概念。有些螃蟹还把注射了酸液的腿扔掉。因为这种行为可能与疼痛有关,所以也可能是由疼痛引起的。先前注射吗啡会引起各种行为变化,但吗啡并不能改善对醋酸的反应。因此,吗啡并不具有镇痛作用,这与之前的研究结果一致。尽管如此,向腿部注射吗啡后出现的定向行为与其他研究结果一致,并提供了更多证据表明这些动物会感到疼痛。摘要 在脊椎动物的疼痛研究中经常使用有毒化学物质和吗啡治疗。在这里,我们展示了注射吗啡会导致螃蟹(Carcinus maenas)的一些行为变化,包括减少对围栏两侧的挤压、更多地摩擦和抠嘴部,以及至少在短时间内更多的防御性表现。随后向一条后腿注射醋酸会引起被注射腿的摩擦,被注射的腿会垂直离开地面。这些针对特定腿部或涉及特定腿部的活动与以前观察到的有害刺激后的定向行为一致,也符合十足目动物经历疼痛的观点。此外,醋酸而非水的注射会诱导这些动物对注射腿进行自切。由于自切与定向行为在时间上相关,因此自切可能是一种与疼痛相关的反应。醋酸显然是一种对十足目动物有害的物质。然而,吗啡对与注射醋酸相关的活动没有影响,因此没有证据表明吗啡有镇痛作用。此外,注射醋酸不会干扰吗啡的行为效应。针对注射部位的活动与注射或外部施用各种有害物质时观察到的活动相似,本研究为有关十足目动物可能的疼痛的知识库增添了新的内容。
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引用次数: 0
Copra Meal: A Review of Its Production, Properties, and Prospects 科普拉粕:Copra Meal:生产、特性和前景综述
Pub Date : 2024-06-01 DOI: 10.3390/ani14111689
Jan Kathleen M. Punzalan, K. Rosentrater
Simple Summary There has been a growing focus on the utilization of copra meal, the byproduct of coconut oil production, as an alternative feed ingredient to supplement the growing demands of the ruminant livestock, poultry, and aquaculture industries. It provides a locally available and inexpensive source of protein, which is essential in tropical and subtropical regions, where coconut production is robust while conventional feed ingredients are scarce and prices are often unstable. This paper presents up-to-date information on the advancements in using copra meal as an animal feed ingredient and the various methods employed to enhance its nutritional and feeding value. It also offers a comprehensive overview of the physical and chemical properties of copra meal, emphasizing the need for further research to fully exploit its potential due to the limited understanding of its physical properties and the variability in quality resulting from different processing operations. This review paper also focuses on other potential applications for copra meal, which is crucial given the substantial global production volume with limited value addition. This could play a significant role in boosting the profitability of the coconut sector, reducing agricultural waste, and promoting sustainable agricultural practices. Abstract Copra meal, the byproduct of coconut oil production, has been widely available at low cost but has been underutilized, with huge portions still becoming waste. Extensive research on different species have been performed to improve its use as an alternative feed ingredient, aiming to reduce the impact of fluctuating feed prices in some parts of the world where coconut is a major commodity. As for any biological product, the physical and chemical properties of copra meal play a crucial role in its use and limitations. In the case of copra meal, studies have found that additional treatments are needed to improve its nutritional composition and make it readily and efficiently available for ruminant and monogastric animals, poultry, and aquaculture applications. This paper presents a summary of up-to-date information on the physical and chemical characteristics of the product, as well as discussions on the various methods employed to improve and optimize its biological value as animal feed. There have been limited studies that have explored other effective and economical means of utilizing copra meal outside the livestock and feed industry. Hence, this paper also aims to provide a lens on future prospects and diverse applications involving copra meal, as well as to present the gaps and challenges that have to be addressed to maximize its product value and biological potential.
简单摘要 人们越来越重视利用椰子油生产的副产品椰肉粉作为替代饲料原料,以补充反刍家畜、家禽和水产养殖业日益增长的需求。它提供了一种就地取材且价格低廉的蛋白质来源,这在椰子产量旺盛而传统饲料原料稀缺且价格往往不稳定的热带和亚热带地区至关重要。本文介绍了将椰肉粉用作动物饲料原料的最新进展,以及提高其营养和饲养价值的各种方法。本文还全面概述了椰干粉的物理和化学特性,强调由于对其物理特性的了解有限以及不同加工操作导致的质量差异,需要进一步研究以充分挖掘其潜力。本综述论文还重点介绍了椰干粕的其他潜在应用,鉴于全球产量巨大但附加值有限,这一点至关重要。这对于提高椰子产业的盈利能力、减少农业废弃物和促进可持续农业实践具有重要作用。摘要 椰子油生产过程中产生的副产品--椰肉粉(Copra meal)一直以低廉的价格广泛供应,但一直未得到充分利用,仍有大量椰肉粉成为废物。人们对不同物种进行了广泛的研究,以改善其作为替代饲料原料的用途,目的是在世界上椰子是主要商品的一些地区减少饲料价格波动的影响。与任何生物产品一样,椰肉粉的物理和化学特性对其用途和局限性起着至关重要的作用。就椰肉粉而言,研究发现需要进行额外处理,以改善其营养成分,并使其能够方便有效地用于反刍动物、单胃动物、家禽和水产养殖。本文概述了有关该产品物理和化学特性的最新信息,并讨论了为改善和优化其作为动物饲料的生物价值而采用的各种方法。除畜牧业和饲料行业外,对其他有效和经济的椰干粕利用方法的研究还很有限。因此,本文还旨在透视椰干粉的未来前景和各种应用,并提出为最大限度地提高其产品价值和生物潜力而必须解决的差距和挑战。
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引用次数: 0
Genome-Wide Gene–Environment Interaction Analysis Identifies Novel Candidate Variants for Growth Traits in Beef Cattle 全基因组基因与环境相互作用分析发现肉牛生长性状的新型候选变异基因
Pub Date : 2024-06-01 DOI: 10.3390/ani14111695
Tianyu Deng, Keanning Li, Lili Du, Mang Liang, Li Qian, Qingqing Xue, Shiyuan Qiu, Lingyang Xu, Lupei Zhang, Xue Gao, Xianyong Lan, Junya Li, Huijiang Gao
Simple Summary Growth traits have been widely studied as economically important traits in the beef cattle industry. However, traditional studies often miss how these traits change under different environmental conditions, only focusing on single genetic changes that affect traits directly. In our study, we analyzed how genetics and environment interact to affect growth in beef cattle, considering four growth traits and two environmental factors. This analysis uncovered several genetic markers for growth traits that are not usually evident in standard studies, showing that some genes have effects that can be obliterated by environmental conditions Further testing showed whether these genetic markers are grouped in specific genes or functional pathways, helping us understand how genetics can influence growth under different environmental conditions. By uncovering novel genetic loci, genes, and candidate biological mechanisms associated with growth traits, our study provides valuable information for selection prediction and breeding decisions in the beef cattle industry. Abstract Complex traits are widely considered to be the result of a compound regulation of genes, environmental factors, and genotype-by-environment interaction (G × E). The inclusion of G × E in genome-wide association analyses is essential to understand animal environmental adaptations and improve the efficiency of breeding decisions. Here, we systematically investigated the G × E of growth traits (including weaning weight, yearling weight, 18-month body weight, and 24-month body weight) with environmental factors (farm and temperature) using genome-wide genotype-by-environment interaction association studies (GWEIS) with a dataset of 1350 cattle. We validated the robust estimator’s effectiveness in GWEIS and detected 29 independent interacting SNPs with a significance threshold of 1.67 × 10−6, indicating that these SNPs, which do not show main effects in traditional genome-wide association studies (GWAS), may have non-additive effects across genotypes but are obliterated by environmental means. The gene-based analysis using MAGMA identified three genes that overlapped with the GEWIS results exhibiting G × E, namely SMAD2, PALMD, and MECOM. Further, the results of functional exploration in gene-set analysis revealed the bio-mechanisms of how cattle growth responds to environmental changes, such as mitotic or cytokinesis, fatty acid β-oxidation, neurotransmitter activity, gap junction, and keratan sulfate degradation. This study not only reveals novel genetic loci and underlying mechanisms influencing growth traits but also transforms our understanding of environmental adaptation in beef cattle, thereby paving the way for more targeted and efficient breeding strategies.
简单摘要 生长性状是肉牛业中具有重要经济价值的性状,已被广泛研究。然而,传统的研究往往忽略了这些性状在不同环境条件下的变化,而只关注直接影响性状的单一遗传变化。在我们的研究中,我们分析了遗传和环境如何相互作用影响肉牛的生长,考虑了四个生长性状和两个环境因素。这项分析发现了几个在标准研究中通常并不明显的生长性状遗传标记,表明有些基因的影响可能会被环境条件所抹杀。进一步的测试表明,这些遗传标记是否归类于特定的基因或功能通路,帮助我们了解遗传如何在不同的环境条件下影响生长。通过发现与生长性状相关的新遗传位点、基因和候选生物学机制,我们的研究为肉牛业的选择预测和育种决策提供了宝贵的信息。摘要 复杂性状被广泛认为是基因、环境因素和基因型与环境相互作用(G × E)的复合调控结果。将 G × E 纳入全基因组关联分析对于了解动物的环境适应性和提高育种决策的效率至关重要。在此,我们利用 1350 头牛的数据集,通过全基因组基因型与环境交互关联研究(GWEIS)系统地研究了生长性状(包括断奶体重、一岁体重、18 个月体重和 24 个月体重)与环境因素(农场和温度)的 G × E。我们验证了稳健估计器在 GWEIS 中的有效性,并检测到 29 个独立的互作 SNPs,显著性阈值为 1.67 × 10-6,表明这些在传统全基因组关联研究(GWAS)中未显示主效应的 SNPs 可能在不同基因型之间具有非加性效应,但被环境因素所湮没。使用 MAGMA 进行的基于基因的分析发现了三个与 GEWIS 结果重叠的基因,即 SMAD2、PALMD 和 MECOM。此外,基因组分析中的功能探索结果揭示了牛生长如何对环境变化做出反应的生物机制,如有丝分裂或细胞分裂、脂肪酸β氧化、神经递质活性、间隙连接和角蛋白硫酸盐降解。这项研究不仅揭示了影响生长性状的新遗传位点和潜在机制,而且改变了我们对肉牛环境适应性的认识,从而为制定更有针对性和更有效的育种策略铺平了道路。
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引用次数: 0
No Evidence of Neutrophil Response Modulation in Goats after Immunization against Paratuberculosis with a Heat-Inactivated Vaccine 用热活化疫苗免疫副结核病后,没有证据表明山羊的中性粒细胞反应发生了改变
Pub Date : 2024-06-01 DOI: 10.3390/ani14111694
M. Criado, Marta Silva, Pedro Mendívil, E. Molina, Valentín Pérez, Julio Benavides, N. Elguezabal, D. Gutiérrez-Expósito
Simple Summary Among other limitations, the currently available vaccines against paratuberculosis do not offer complete protection against infection, and further vaccine development is limited by a lack of understanding of the mechanisms behind vaccine-induced protection. In this regard, the most recent studies have demonstrated that neutrophil function can be modulated through vaccination against several pathogens, including Mycobacterium avium subspecies paratuberculosis (Map). However, this modulation has not been described in ruminants, which are the natural hosts of Map. In the present work, the effect of vaccination on the neutrophil response against Map was assessed in goats using the only available vaccine against small ruminant paratuberculosis, Gudair®. No differences were found in the ex vivo response of neutrophils isolated from non-vaccinated and vaccinated animals, which suggests that the protection conferred by this heat-inactivated vaccine is based on mechanisms other than neutrophil modulation. It is possible that neutrophil modulation depends largely on the intensity of the immune response elicited by the vaccine employed or the antigen dose, as the previous reports which observed this modulation used live attenuated vaccines or were performed in laboratory animals using experimental vaccines. Abstract Neutrophils are believed to play a role in the initial stages of paratuberculosis, and it has recently been demonstrated that vaccination can modulate their function via priming or through epigenetic and metabolic reprogramming (training). Modulation of the neutrophil response against Mycobacterium avium subspecies paratuberculosis (Map) through vaccination has been demonstrated in a rabbit model but not in ruminants. Therefore, in the present work, the effect of vaccination on the response of caprine neutrophils against Map was studied. Neutrophils were isolated from non-vaccinated (n = 7) and Gudair®-vaccinated goat kids (n = 7), before vaccination and 30 days post-vaccination. Then, several neutrophil functions were quantified ex vivo: cell-free and anchored neutrophil extracellular trap (NET) release, phagocytosis, and the differential expression of several cytokines and TLR2. The induction of cell-free NETosis and TLR2 expression by Map is reported for the first time. However, vaccination showed no significant effect on any of the functions studied. This suggests that the protection conferred by Gudair® vaccination is based on mechanisms that are independent of the neutrophil function modulation. Further research into the impact of alternative vaccination strategies or the paratuberculosis infection stage on ruminant neutrophil function could provide valuable insights into its role in paratuberculosis.
简单摘要 除其他局限性外,目前可用的副结核病疫苗并不能提供完全的抗感染保护,疫苗的进一步开发也因缺乏对疫苗诱导保护机制的了解而受到限制。在这方面,最新的研究表明,中性粒细胞的功能可以通过接种疫苗来调节,以预防包括副结核分枝杆菌(Map)在内的多种病原体的感染。然而,在反刍动物(Map 的天然宿主)中还没有关于这种调节的描述。在本研究中,使用唯一可用的小反刍动物副结核病疫苗 Gudair®,评估了接种疫苗对山羊中性粒细胞对 Map 的反应的影响。从未接种疫苗和接种疫苗的动物体内分离出的中性粒细胞的体内外反应没有发现差异,这表明这种热灭活疫苗所提供的保护是基于中性粒细胞调节以外的机制。中性粒细胞的调节作用可能在很大程度上取决于所使用疫苗或抗原剂量引起的免疫反应的强度,因为以前观察到这种调节作用的报道都是使用减毒活疫苗或在使用实验疫苗的实验动物身上进行的。摘要 中性粒细胞被认为在副结核病的初期阶段发挥了作用,最近的研究表明,接种疫苗可通过引物或通过表观遗传学和代谢重编程(训练)调节中性粒细胞的功能。通过接种疫苗调节中性粒细胞对副结核分枝杆菌(Map)的反应已在兔子模型中得到证实,但在反刍动物中尚未得到证实。因此,在本研究中,研究人员研究了接种疫苗对牛中性粒细胞对 Map 的反应的影响。在接种前和接种后 30 天,从未接种疫苗(n = 7)和接种 Gudair® 疫苗的山羊幼崽(n = 7)中分离出中性粒细胞。然后,对几种中性粒细胞功能进行了体内外量化:无细胞和固定的中性粒细胞胞外捕获器(NET)释放、吞噬以及几种细胞因子和 TLR2 的不同表达。这是首次报道马普诱导无细胞NETosis和TLR2的表达。然而,疫苗接种对所研究的任何功能都没有明显影响。这表明接种 Gudair® 疫苗所提供的保护是基于独立于中性粒细胞功能调节的机制。进一步研究其他疫苗接种策略或副结核病感染阶段对反刍动物中性粒细胞功能的影响,可以为了解中性粒细胞在副结核病中的作用提供有价值的信息。
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引用次数: 0
Prevalence and Genetic Characterization of Porcine Respiratory Coronavirus in Korean Pig Farms 韩国养猪场猪呼吸道冠状病毒的流行情况和遗传特征
Pub Date : 2024-06-01 DOI: 10.3390/ani14111698
Ju-Han Kim, Jonghyun Park, Dong-Kyu Lee, Won-Il Kim, Y. Lyoo, Choi-Kyu Park, Hye-Ryung Kim
Simple Summary The current epidemiology of porcine respiratory coronavirus (PRCV) in domestic pig farms in the Republic of Korea is not well understood. In this study, PRCV was found to still be present in Korean pig herds with a high seroprevalence. Genetic and phylogenetic analyses of PRCV S gene sequences suggested that Korean PRCV originated from European PRCV and has evolved in Korea. These findings will help expand knowledge about the epidemiology and genetic characteristics of PRCV in Korea. Abstract Porcine respiratory coronavirus (PRCV) is a member of the species Alphacoronavirus 1 within the genus Alphacoronavirus of the family Coronaviridae. A few studies have been conducted on the prevalence of PRCV since its first identification in 1997, but there have been no recent studies on the prevalence and genetic characterization of the virus in Korea. In this study, the seroprevalence of PRCV was determined in Korean pig farms using a commercially available TGEV/PRCV differential enzyme-linked immunosorbent assay kit. The farm-level seroprevalence of PRCV was determined to be 68.6% (48/70), similar to previous reports in Korea, suggesting that PRCV is still circulating in Korean pig herds nationwide. Among the 20 PRCV-seropositive farms tested in this study, PRCV RNAs were detected in 17 oral fluid samples (28.3%) from nine farms (45.0%), while TGEV RNAs were not detected in any sample. To investigate the genetic characteristics of Korean PRCV strains, genetic and phylogenetic analyses were conducted on PRCV spike gene sequences obtained in this study. The three Korean PRCV strains (KPRCV2401, KPRCV2402, and KPRCV2403) shared 98.5–100% homology with each other and 96.2–96.6% and 91.6–94.5% homology with European and American strains, respectively. A 224-amino acid deletion was found in the S gene of both Korean and European PRCVs but not in that of American PRCVs, suggesting a European origin for Korean PRCVs. Phylogenetic analysis showed that Korean PRCVs are more closely related to European PRCVs than American PRCVs but clustered apart from both, suggesting that Korean PRCV has evolved independently since its emergence in Korean PRCVs. The results of this study will help expand knowledge on the epidemiology and molecular biology of PRCV currently circulating in Korea.
简单摘要 猪呼吸道冠状病毒(PRCV)目前在大韩民国国内养猪场的流行病学尚不十分清楚。本研究发现,猪呼吸道冠状病毒(PRCV)仍然存在于韩国猪群中,且血清流行率很高。PRCV S 基因序列的遗传和系统发育分析表明,韩国的 PRCV 起源于欧洲的 PRCV,并在韩国发生了进化。这些发现将有助于扩大对韩国PRCV流行病学和遗传特征的了解。摘要 猪呼吸道冠状病毒(PRCV)是冠状病毒科 Alphacoronavirus 属 Alphacoronavirus 1 种的一个成员。自 1997 年首次发现 PRCV 以来,已对其流行情况进行了一些研究,但近期尚未对该病毒在韩国的流行情况和遗传特征进行研究。本研究使用市售的 TGEV/PRCV 差异酶联免疫吸附测定试剂盒测定了韩国猪场的 PRCV 血清流行率。经测定,猪场一级的 PRCV 血清流行率为 68.6%(48/70),与韩国以前的报告相似,表明 PRCV 仍在韩国全国的猪群中流行。在本研究检测的 20 个 PRCV 血清阳性猪场中,从 9 个猪场(45.0%)的 17 份口腔液样本(28.3%)中检测到 PRCV RNA,而在任何样本中均未检测到 TGEV RNA。为研究韩国PRCV毒株的遗传特征,本研究对获得的PRCV尖峰基因序列进行了遗传和系统发育分析。三个韩国 PRCV 株系(KPRCV2401、KPRCV2402 和 KPRCV2403)之间的同源性为 98.5%-100%,与欧洲和美国株系的同源性分别为 96.2%-96.6%和 91.6%-94.5%。在韩国和欧洲的PRCV的S基因中都发现了224个氨基酸的缺失,而在美国的PRCV中却没有发现,这表明韩国的PRCV起源于欧洲。系统进化分析表明,韩国PRCV与欧洲PRCV的亲缘关系比与美国PRCV的亲缘关系更近,但与两者的聚类关系不同,这表明韩国PRCV自出现在韩国PRCV后一直在独立进化。这项研究的结果将有助于扩大目前在韩国流行的PRCV的流行病学和分子生物学知识。
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引用次数: 0
Livestock Depredation by Large Carnivores and Human–Wildlife Conflict in Two Districts of Balochistan Province, Pakistan 巴基斯坦俾路支省两个地区大型食肉动物对牲畜的掠夺以及人与野生动物之间的冲突
Pub Date : 2024-04-01 DOI: 10.3390/ani14071104
N. Ullah, I. Basheer, F. Rehman, Minghai Zhang, Muhammad Tayyab Khan, Sanaullah Khan, Hairong Du
Simple Summary Livestock herding is an essential and time-honored practice in Balochistan, playing a pivotal role in the region’s economy, culture, and way of life. The livestock sector is of utmost important in the province, catering to nearly 20% of the national stock. Over centuries, large predators and their prey species, including livestock, have coexisted in these mountainous landscapes. Consequently, large carnivores are more likely to interact with humans due to their extensive home ranges. This research aims to explore the impact of livestock depredation by large predators on livelihoods and conservation efforts in two districts of Balochistan, Pakistan. A human–carnivore conflict survey was conducted from July to September 2019, gathering data from 311 residents in the selected study area. Large predators in the region preyed on a total of 876 livestock over a year, comprising 560 goats, 292 sheep, 19 cows, and 5 donkeys. The gray wolf emerged as the primary predator, accounting for 66.3% of livestock depredation, which was followed by the caracal (24.3%), Asiatic jackal (8.9%), and striped hyena (0.6%). The economic loss totaled USD 78,694. Notably, 80% of respondents held negative perceptions toward wolves compared to 24.4% for caracals. Only 20.6% of respondents were aware of the importance of conserving carnivores. Livestock depredation by carnivores has fostered negative perceptions among locals toward these animals. There exists a lack of awareness regarding the significance of conserving carnivore species and their ecological roles. It is crucial to raise awareness among communities about the ecological importance of predators like the gray wolf, caracal, Asiatic jackal, striped hyena, and Balochistan black bear through community meetings and educational seminars. Furthermore, providing basic education to herders on effective livestock guarding practices is recommended to mitigate human–carnivore conflicts and promote coexistence between wildlife and local communities in Balochistan. Abstract Livestock herding is a vital practice in Balochistan, contributing to the economy and culture. The livestock sector is significant in Balochistan, providing 20% of the national stock. Large predators and their prey species, including livestock, have coexisted in these mountainous landscapes for centuries. The aim of the present research is to investigate the impacts of livestock depredation by large predators on livelihoods and predator conservation in two districts of Balochistan, Pakistan. A human–carnivore conflict survey was conducted from July to September 2019, collecting data from 311 residents in a selected study area. Large predators in the study area preyed on a total of 876 livestock during a one-year period, including 560 goats, 292 sheep, 19 cows, and 5 donkeys. The gray wolf is the leading predator, responsible for 66.3% of livestock depredation, followed by the caracal (24.3%), Asiatic jackal (8.9%), and striped hyena (0.6%).
简单摘要 在俾路支省,畜牧业是一种重要而历史悠久的习俗,在该地区的经济、文化和生活方式中发挥着举足轻重的作用。畜牧业在俾路支省极为重要,其牲畜存栏量占全国的近 20%。几个世纪以来,大型食肉动物及其猎物物种(包括牲畜)一直在这些山区地貌中共存。因此,大型食肉动物因其广泛的家园范围而更有可能与人类发生互动。本研究旨在探讨大型食肉动物捕食牲畜对巴基斯坦俾路支省两个地区的生计和保护工作的影响。研究人员于 2019 年 7 月至 9 月开展了一项人类与食肉动物冲突调查,收集了所选研究地区 311 名居民的数据。一年来,该地区的大型食肉动物共捕食了 876 头牲畜,其中包括 560 只山羊、292 只绵羊、19 头奶牛和 5 头驴。灰狼是最主要的掠食者,占牲畜捕食量的 66.3%,其次是狞獾(24.3%)、亚洲豺(8.9%)和条纹鬣狗(0.6%)。经济损失共计 78,694 美元。值得注意的是,80% 的受访者对狼持有负面看法,而对狞猫持有负面看法的受访者仅占 24.4%。只有 20.6% 的受访者意识到保护食肉动物的重要性。食肉动物对牲畜的掠夺使当地人对这些动物产生了负面看法。人们对保护食肉动物物种的意义及其生态作用缺乏认识。通过社区会议和教育研讨会提高社区对灰狼、狞猫、亚洲豺、条纹鬣狗和俾路支黑熊等食肉动物生态重要性的认识至关重要。此外,还建议向牧民提供有关有效牲畜看护方法的基础教育,以缓解人与食肉动物之间的冲突,促进野生动物与俾路支省当地社区的共存。摘要 畜牧业是俾路支省的一项重要活动,对经济和文化都有贡献。畜牧业在俾路支省非常重要,占全国牲畜存栏量的 20%。几个世纪以来,大型食肉动物及其猎物物种(包括牲畜)一直在这些山区地貌中共存。本研究旨在调查大型食肉动物捕食牲畜对巴基斯坦俾路支省两个地区的生计和食肉动物保护的影响。研究人员于 2019 年 7 月至 9 月开展了一项人类与食肉动物冲突调查,收集了选定研究地区 311 名居民的数据。在一年的时间里,研究地区的大型食肉动物共捕食了 876 头牲畜,其中包括 560 只山羊、292 只绵羊、19 头奶牛和 5 头驴。灰狼是最主要的掠食者,占牲畜捕食总量的 66.3%,其次是狞獾(24.3%)、亚洲豺(8.9%)和条纹鬣狗(0.6%)。经济损失总额为 78,694 美元。总体而言,80% 的受访者对狼持负面看法,而对狞猫持负面看法的受访者仅占 24.4%。只有 20.6% 的受访者知道保护食肉动物的重要性。在研究地区,食肉动物对牲畜的掠夺造成了人们对这些动物的负面看法。人们对保护食肉动物物种的重要性及其在生态系统中的作用缺乏认识。这种认识不足最终导致食肉动物种群受到有害影响。当务之急是通过社区会议、在教育机构举办研讨会以及向牧民提供有关有效保护牲畜的基本教育,提高人们对食肉动物生态意义的认识。
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引用次数: 0
Characteristics of Buffalo Farming Systems in Turkey Based on a Multivariate Aggregation of Indicators: A Survey Study. 基于多元指标聚合的土耳其水牛养殖系统特征调查研究
IF 3 Pub Date : 2022-11-07 DOI: 10.3390/ani12213056
Nursen Ozturk, Omur Kocak, Arzu Peker, Lorenzo Serva, Ferhan Kaygisiz, Pembe Dilara Kecici, Hulya Yalcintan, Halil Ibrahim Kilic, Luisa Magrin

This study aimed to determine the heterogeneity that exists in water buffalo husbandry systems in Marmara Region, Turkey. A questionnaire containing a total of 60 indicators was submitted to 52 farmers. A Principal Component Analysis was performed to reduce original variables into a simplified and latent structure, which was characterized by six orthogonal components: milk productivity, economic efficiency, roughage management, dual-purpose farming, concentrate supply, and fodder production. An ANOVA model was applied to the six components to investigate the effects of the province, investment levels, grazing type, milk production, and profitability. Differences in milk productivity, roughage management, concentrate supply, and fodder management were significant according to the province and grazing type, which indicated a difference in intensification levels among the cities. Economic efficiency and dual-purpose farming differed significantly for milk production levels as well as milk productivity, and economic efficiency differed for profitability levels. We found a tendency regarding the impact of roughage management on profitability. The results conclude that profitability was associated with improving the milking traits of buffaloes and roughage management of the farms.

本研究旨在确定土耳其马尔马拉地区水牛养殖系统中存在的异质性。向52名农民提交了一份载有60项指标的调查表。通过主成分分析,将原始变量简化为由产奶量、经济效益、粗饲料管理、双重用途养殖、精料供应和饲料产量6个正交分量表征的简化潜在结构。采用方差分析(ANOVA)模型对6个组成部分进行分析,考察省区、投资水平、放牧类型、产奶量和盈利能力的影响。奶牛产奶量、粗饲料管理、精料供应和饲料管理在不同省份和不同放牧类型间存在显著差异,表明各城市集约化程度存在差异。经济效率和双重用途养殖在产奶量和产奶量上存在显著差异,在盈利水平上存在显著差异。我们发现了粗饲料管理对盈利能力影响的趋势。结果表明,盈利能力与改善水牛的挤奶特性和农场的粗饲料管理有关。
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引用次数: 0
Effect of Anionic and Zeolite Supplements and Oral Calcium Bolus in Prepartum Diets on Feed Intake, Milk Yield and Milk Compositions, Plasma Ca Concentration, Blood Metabolites and the Prevalence of Some Reproductive Disorders in Fresh Dairy Cows. 预备饲粮中添加阴离子和沸石及口服钙丸对鲜奶牛采食量、产奶量和乳成分、血浆钙浓度、血液代谢物及部分生殖障碍发生率的影响
IF 3 Pub Date : 2022-11-07 DOI: 10.3390/ani12213059
Mohammad Mahdi Masoumi Pour, Farhad Foroudi, Naser Karimi, Mohammad Reza Abedini, Kazem Karimi

Hypocalcaemia is an important disorder associated with an increased risk of metabolic diseases, and many studies have been going on for decades. This study investigated the effects of anionic and zeolite with an oral Ca bolus in the prepartum phase on milk yields and compositions, and plasma Ca concentrations, blood metabolites and the prevalence of some reproductive disorders in dairy cows after parturition. Ninety pregnant non-lactating Holstein-Friesian cows were randomly assigned to three isoenergetic diets and their counterparts using an oral Ca bolus: (1) Control (CON): low Ca (0.44%); (2) Anionic (ANI): high Ca (1.00%); (3) Zeolite (ZEO): low Ca (0.44%). The feed and energy intakes of the CON and ZEO groups were higher than the ANI group. The raw milk yield, Pr%, and feed efficiency did not differ between the groups, while the corrected milk yield, fat% and total corrected milk yield at 305 days differed between groups. Total plasma and the ionized Ca concentration of the ANI and ZEO groups, at calving time and in 6, 12, 24 and 48 h after that, were higher than in the CON group. The frequencies of hypocalcaemia and reproductive disorders in the ANI and ZEO groups were lower than in the CON. The blood metabolite levels in the CON group were higher than in other groups. In conclusion, the ANI and ZEO diets and their counterparts supplemented with an oral Ca bolus improved the milk production traits, plasma calcium and metabolites concentration and also effectively prevented reproductive disorders.

低钙血症是一种与代谢疾病风险增加相关的重要疾病,许多研究已经进行了几十年。本试验研究了阴离子钙和沸石钙预备期口服钙丸对奶牛产奶量和乳成分、血浆钙浓度、血液代谢物以及分娩后某些生殖障碍发生率的影响。90头妊娠非哺乳期荷斯坦-弗里西亚奶牛随机分为3种等能饲粮和口服钙丸组:(1)对照(CON):低钙(0.44%);(2)阴离子(ANI):高Ca (1.00%);(3)沸石(ZEO):低钙(0.44%)。CON组和ZEO组的采食量和能量采食量均高于ANI组。原料产奶量、Pr%和饲料效率各组间无显著差异,305 d校正产奶量、脂肪%和总校正产奶量组间无显著差异。ANI组和ZEO组产犊时及产犊后6、12、24、48 h的总血浆浓度和电离钙浓度均高于CON组。ANI组和ZEO组低钙血症和生殖障碍发生率低于CON组,CON组血液代谢物水平高于其他组。综上所述,ANI和ZEO饲粮及相应饲粮中添加口服钙丸改善了泌乳性状、血浆钙和代谢物浓度,并有效预防了生殖障碍。
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引用次数: 0
Insights into the Role of Natural Products in the Control of the Honey Bee Gut Parasite (Nosema spp.). 天然产物在控制蜜蜂肠道寄生虫中的作用。
IF 3 Pub Date : 2022-11-07 DOI: 10.3390/ani12213062
Hesham R El-Seedi, Aida A Abd El-Wahed, Yahya Al Naggar, Aamer Saeed, Jianbo Xiao, Hammad Ullah, Syed G Musharraf, Mohammad H Boskabady, Wei Cao, Zhiming Guo, Maria Daglia, Abeer El Wakil, Kai Wang, Shaden A M Khalifa

The honey bee is an important economic insect due to its role in pollinating many agricultural plants. Unfortunately, bees are susceptible to many pathogens, including pests, parasites, bacteria, and viruses, most of which exert a destructive impact on thousands of colonies. The occurrence of resistance to the therapeutic substances used against these organisms is rising, and the residue from these chemicals may accumulate in honey bee products, subsequently affecting the human health. There is current advice to avoid the use of antibiotics, antifungals, antivirals, and other drugs in bees, and therefore, it is necessary to develop alternative strategies for the treatment of bee diseases. In this context, the impact of nosema diseases (nosemosis) on bee health and the negative insults of existing drugs are discussed. Moreover, attempts to combat nosema through the use of alternative compounds, including essential oils, plant extracts, and microbes in vitro and in vivo, are documented.

蜜蜂是一种重要的经济昆虫,因为它为许多农业植物授粉。不幸的是,蜜蜂容易受到许多病原体的影响,包括害虫、寄生虫、细菌和病毒,其中大多数对成千上万的蜂群产生破坏性影响。对用于治疗这些生物的治疗物质产生耐药性的情况正在增加,这些化学物质的残留物可能积聚在蜜蜂产品中,从而影响人类健康。目前的建议是避免在蜜蜂身上使用抗生素、抗真菌药物、抗病毒药物和其他药物,因此,有必要制定治疗蜜蜂疾病的替代策略。在此背景下,鼻塞病(鼻塞病)对蜜蜂健康的影响和现有药物的负面损害进行了讨论。此外,通过使用替代化合物(包括精油、植物提取物和体外和体内微生物)来对抗鼻塞的尝试也有记录。
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引用次数: 5
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Animals : an Open Access Journal from MDPI
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