Extracellular vesicles and circulating nucleic acids最新文献

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Erratum: Breaking free: endocytosis and endosomal escape of extracellular vesicles. 勘误：脱离：胞外囊泡的内吞作用和内体逃逸。

Extracellular vesicles and circulating nucleic acids

Pub Date : 2023-09-12 eCollection Date: 2023-01-01 DOI: 10.20517/evcna.2023.42

Laís Ribovski, Bhagyashree Joshi, Jie Gao, Inge Zuhorn

[This corrects the article DOI: 10.20517/evcna.2023.26.].

[这更正了文章DOI: 10.20517/evcna.2023.26.]。

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Erratum: Identification of important extracellular vesicle RNA molecules related to sperm motility and prostate cancer. 勘误：鉴定与精子运动和前列腺癌相关的重要细胞外囊泡RNA分子。

Extracellular vesicles and circulating nucleic acids

Pub Date : 2023-07-11 eCollection Date: 2023-01-01 DOI: 10.20517/evcna.2023.35

Evcna Editorial Office

[This corrects the article DOI: 10.20517/evcna.2021.02.].

[这更正了文章DOI: 10.20517/evcna.2021.02.]。

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Acknowledgement to Reviewers of Extracellular Vesicles and Circulating Nucleic Acids in 2022. 向2022年《细胞外囊泡与循环核酸》审稿人致谢。

Extracellular vesicles and circulating nucleic acids

Pub Date : 2023-01-28 eCollection Date: 2023-01-01 DOI: 10.20517/evcna.2023.02

Evcna Editorial Office

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Year-end reflections of EVCNA - 2022. EVCNA - 2022的年终反思。

Extracellular vesicles and circulating nucleic acids

Pub Date : 2022-12-30 eCollection Date: 2022-01-01 DOI: 10.20517/evcna.2022.45

Yoke Peng Loh

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Mesenchymal stem cell-derived extracellular vesicles for cell-free therapy of ocular diseases. 间充质干细胞来源的细胞外囊泡用于眼部疾病的无细胞治疗。

Extracellular vesicles and circulating nucleic acids

Pub Date : 2022-04-24 eCollection Date: 2022-01-01 DOI: 10.20517/evcna.2022.08

Xiaoling Liu, Liang Hu, Fei Liu

Mesenchymal stem cells-derived extracellular vesicles (MSC-EVs) have noticeably attracted clinicians' attention in treating ocular diseases. As the paracrine factor of MSCs and an alternative for cell-free therapies, MSC-EVs can be conveniently dropped over the ocular surface or diffused through the retina upon intravitreal injection, without increasing the risks of cellular rejection and tumor formation. For clinical translation, a standardized and scalable production, as well as reprogramming the MSC-EVs, are highly encouraged. This review aims to assess the potential approaches for EV production and functional modification, in addition to summarizing the worldwide clinical trials initiated for various physiological systems and the specific biochemical effects of MSC-EVs on the therapy of eye diseases. Recent advances in the therapy of ocular diseases based on MSC-EVs are reviewed, and the associated challenges and prospects are discussed as well.

间充质干细胞衍生的细胞外囊泡（MSC-EVs）在眼部疾病的治疗中引起了临床医生的极大关注。作为MSCs的旁分泌因子和无细胞治疗的替代方案，MSCs - ev可以方便地滴入眼表或通过玻璃体内注射通过视网膜扩散，而不会增加细胞排斥和肿瘤形成的风险。对于临床翻译，高度鼓励标准化和可扩展的生产，以及重新编程msc - ev。本文综述了MSC-EVs在世界范围内针对各种生理系统开展的临床试验以及其在眼病治疗中的具体生化作用，并对其生产和功能修饰的潜在途径进行了综述。本文综述了基于msc - ev的眼部疾病治疗的最新进展，并讨论了相关的挑战和前景。

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Assessing extracellular vesicles from bovine mammary gland epithelial cells cultured in FBS-free medium. 在无fbs培养基中培养的牛乳腺上皮细胞细胞外囊泡的评价。

Extracellular vesicles and circulating nucleic acids

Pub Date : 2021-12-31 eCollection Date: 2021-01-01 DOI: 10.20517/evcna.2021.18

Giulia Silvestrelli, Susanne E Ulbrich, Mara D Saenz-de-Juano

Aim: Mammary gland extracellular vesicles (EVs) are found in both human and livestock milk. Our knowledge of the role of EVs in the mammary gland development, breast cancer and mastitis derives mainly from in vitro cell culture models. However, a commonly shared limitation is the use of fetal bovine serum (FBS) as a supplement, which naturally contains EVs. For this reason, the purpose of the study was to evaluate novel tools to investigate mammary gland EVs in vitro and in a FBS-free system.

Methods: Primary bovine mammary epithelial cells (pbMECs) and a mammary gland alveolar epithelial cell line (MAC-T) were cultured in a chemically defined EV-free medium. To find a reliable EV isolation protocol from a starting cell conditioned medium (10 mL), we compared eight different methodologies by combining ultracentrifugation (UC), chemical precipitation (CP), size exclusion chromatography (SEC), and ultrafiltration (UF).

Results: The medium formula sustained both pbMECs and MAC-T cell growth. Transmission electron microscopy revealed that we obtained EV-like particles in five out of eight protocols. The cleanest samples with the highest number of particles and detectable amounts of RNA were obtained by using UF-SEC-UC.

Conclusion: Our chemically defined, FBS-free medium sustains the growth of both pbMECs and MAC-T and allows the isolation of EVs that are free from any contamination by UF-SEC-UC. In conclusion, we propose a new culture system and EVs isolation protocols for further research on mammary epithelial EVs.

目的：乳腺细胞外囊泡（EVs）存在于人乳和家畜乳中。我们对ev在乳腺发育、乳腺癌和乳腺炎中的作用的了解主要来自体外细胞培养模型。然而，一个共同的限制是使用胎牛血清（FBS）作为补充剂，其中天然含有ev。因此，本研究的目的是评估在体外和无fbs系统中研究乳腺EVs的新工具。方法：在化学定义的无ev培养基中培养原代牛乳腺上皮细胞（pbmec）和乳腺肺泡上皮细胞系（MAC-T）。为了从起始细胞条件培养基（10 mL）中找到可靠的EV分离方案，我们比较了8种不同的方法，包括超离心（UC）、化学沉淀（CP）、粒径排除色谱（SEC）和超滤（UF）。结果：培养基能维持pbmec细胞和MAC-T细胞的生长。透射电镜显示，我们在8个方案中的5个方案中获得了ev样粒子。使用UF-SEC-UC获得了颗粒数量最多、RNA可检测量最多的最干净样品。结论：我们的化学定义，无fbs培养基维持pbmec和MAC-T的生长，并允许分离不受任何UF-SEC-UC污染的ev。最后，我们提出了一种新的培养体系和ev的分离方案，为进一步研究乳腺上皮ev提供基础。

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