Zhongguo Zhongyao Zazhi最新文献

Chinese herbal decoction pieces are essential carriers of TCM in clinical practice, and their quality control plays a pivotal role in ensuring therapeutic efficacy and medication safety. However, the current quality evaluation system still relies heavily on empirical assessment and basic physicochemical indicators, resulting in challenges such as inconsistent standards, outdated methodologies, and limited traceability. With the rapid advancement of digital and intelligent technologies, a new paradigm of digital-intelligent quality control, characterized by high-throughput detection, artificial intelligence(AI)-assisted analysis, and full-process modeling, is gradually maturing. This paper systematically reviewed the current state of research and key bottlenecks in the digital-intelligent transformation of quality control for Chinese herbal decoction pieces. Focusing on quality identification technologies, AI-driven analysis, multivariate modeling, and database development, it explored the core components of the technical framework. Typical application scenarios such as geo-authenticity discrimination, adulteration detection, and processing monitoring were also discussed in depth. The quality control of Chinese herbal decoction pieces is undergoing a profound shift from experience-based to data-driven approaches. Digital-intelligent quality control is becoming a key driver for overcoming industrial bottlenecks and promoting high-quality development in the herbal decoction piece industry. The paper proposed future directions in detection technology innovation, data ecosystem construction, and cross-sector integration, aiming to provide theoretical support and technical pathways for the modernization of the quality evaluation system for Chinese herbal decoction pieces.

This study systematically explored the secondary metabolites of an endophytic fungus, Penicillium polonicum, isolated from Emmenopterys henryi. Advanced separation techniques, including silica gel column chromatography, Sephadex LH-20 column chromatography, and semi-preparative liquid chromatography, were combined for compound isolation. Seven compounds were successfully isolated from the rice fermentation products of P. polonicum. Their structures were elucidated by nuclear magnetic resonance spectroscopy, high resolution mass spectrometry, ultraviolet spectrometry, and circular dichroism spectroscopy. The identified compounds were penicimolide A(1), penicimenolide E(2),(3S)-cis-resorcylide(3),(R)-7-hydroxydihydroresorcylide(4),(S)-7-hydroxydihydroresorcylide(5), communesin A(6), and chaetoglobosin C(7). Notably, compound 1 represents a new 12-membered benzenediol lactone. The antifungal activities of these compounds against phytopathogenic fungi was evaluated. The results indicated that compound 1 exhibited weak antifungal activity against Alternaria alternata, with a minimum inhibitory concentration(MIC) of 100 μg·mL~(-1). Compound 6 exhibited moderate antifungal activity against Fusarium graminearum with an MIC value of 25 μg·mL~(-1). Compound 7 demonstrated strong antifungal activity against A. solani and Geotrichum candidum, with MICs of 12.5 μg·mL~(-1).

The clinical efficacy of ischemic stroke is severely limited by the difficulty in targeted delivery of neuroprotective agents across the blood-brain barrier to the ischemic region. Liposomes, with their unique bilayer structure, can carry both hydrophilic and hydrophobic drugs and have excellent biocompatibility, modifiability, and high efficiency in crossing the blood-brain barrier, which have become a key strategy to break through the bottleneck of drug delivery in ischemic stroke treatment. This paper systematically reviews the structural properties of liposomes, their targeted delivery mechanisms, and their cutting-edge applications in ischemic stroke therapy in the past decade, focusing on the precise accumulation of neuroprotective agents in the ischemic region by surface-modified liposomes through the synergistic strategy of active/passive targeting, especially in piggybacking on the active ingredients of TCM. The paper analyzes in depth the latest advances of modified liposomes in inhibiting neuroinflammation, attenuating ischemia/reperfusion injury, combining with tissue plasminogen activator and biomimetic modification of delivery systems. It aims to provide a new theoretical framework for the precise and individualized treatment of ischemic stroke and to provide a reference for the development of neuroprotective agent delivery systems based on liposome technology.

On the basis of the data from the fourth national survey of Chinese materia medica resources and thematic investigations in Guangxi Zhuang Autonomous Region, this study systematically classifies the Chinese materia medica resources in Guangxi by integrating modern taxonomic systems(e.g., APG Ⅳ) with GIS technology, utilizing analytical tools such as Origin 2022, R language, and PAST 4.17. It comprehensively elucidated the family/genus-level composition, geographical distribution, spatiotemporal variations, and conservation status and evaluated the biodiversity of medicinal plant resources. Key findings are summarized as follows. The resource inventory identified a total of 7 542 Chinese materia medica species in Guangxi, which included 6 354 medicinal plant species, 1 021 medicinal animal species, 118 medicinal fungal species, and 49 medicinal minerals. The medicinal plants were dominated by 11 families(e.g., Asteraceae and Fabaceae) and core genera(e.g., Ficus and Rubus). Distribution hotspots were concentrated in northwestern, northern, and central Guangxi, with regional heterogeneity driven by Karst landforms and mountainous habitats. A total of 429 species of rare and endangered medicinal plants belonging to 212 genera of 88 families were identified. Compared with the third national survey, the fourth national survey identified 2 858 new medicinal plant species, 512 new medicinal animal species, and 33 new medicinal fungal species. A systematic analysis was conducted to elucidate the driving forces behind the dynamic changes in medicinal plant resources across Guangxi. This pioneering survey systematically documents the baseline status and biodiversity dynamics of medicinal plant resources in Guangxi, establishing a data foundation for the conservation and sustainable utilization of these resources and the high-quality development of the TCM industry in Guangxi.

This study aims to investigate the mechanism of Fuke Qianjin Capsules in treating pelvic inflammatory disease(PID) based on network pharmacology and animal experiments. The potential targets and signaling pathways of Fuke Qianjin Capsules in regulating PID were predicted by network pharmacology. The mouse model of PID was constructed and administered with different doses of Fuke Qianjin Capsules. After treatment, the uterus tissue of mice was observed for the pathological changes by HE staining. The level of IL-6 and TNF-α in the peripheral blood was measured by ELISA. The protein expression in the TLR4/PI3K/Akt/NF-κB signaling pathways was determined by Western blot. Network pharmacology indicated that a total of 78 compounds, 152 targets, 1 632 PID targets, and 72 intersection targets of "drug-disease" were obtained from Fuke Qianjin Capsules. The main active ingredients included β-sitosterol, stigmasterol, luteolin, and catechin. The key targets included TNF, IL-6, TP53, IL-1β, and CASP3. PI3K/Akt, IL-17, and lipid metabolism/atherosclerosis signaling pathways were mainly involved in the treatment. Animal experiments showed that compared with the model group, Fuke Qianjin Capsules alleviated the uterine inflammatory lesions, reduced pathological damage in the uterus tissue, and decreased the serum level of IL-6 and TNF-α, down-regulated the protein level of TLR4, PI3K, Akt, and NF-κB p65 in PID mice. These findings suggest that Fuke Qianjin Capsules can exert therapeutic effects on PID via multiple components, targets, and pathways. Meanwhile, Fuke Qianjin Capsules may reduce the inflammation and attenuate the uterine injuries of PID mice by inhibiting TLR4/PI3K/Akt/NF-κB signaling pathways.

This study aims to investigate the effects of emodin on proliferation, migration, epithelial-mesenchymal transition(EMT), and autophagy in HONE-1 and CNE-2 cells of nasopharyngeal carcinoma(NPC). HONE-1 and CNE-2 cells were cultured in vitro and divided into control group, low-(7.5 μg·mL~(-1)), and high-(10 μg·mL~(-1)) dose group of emodin. The cells were treated with emodin of different concentrations for 48 h. CCK-8 and EdU experiments detected the proliferation ability of HONE-1 and CNE-2 cells, and clony formation assay detected the clony formation ability of HONE-1 and CNE-2 cells. Transwell assay detected the migration ability of HONE-1 and CNE-2 cells. A stem cell pellet experiment detected the dryness of HONE-1 and CNE-2 cells. Immunofluorescence staining analyzed the expression level of microtubule-associated protein 1 light chain 3β(LC3B) in HONE-1 and CNE-2 cells, and Western blot detected the expression levels of EMT-related proteins and autophagy-related proteins in HONE-1 and CNE-2 cells. The results showed that the IC_(50) of HONE-1 and CNE-2 cells treated with emodin for 48 h were 10.0 and 9.4 μg·mL~(-1), respectively. Compared with the control group, emodin could increase the cell proliferation inhibition rate of HONE-1 and CNE-2 cells, and the effect increased with time or drug dose. Emodin could inhibit the clony formation, migration, and stem cell pelletizing ability of HONE-1 and CNE-2 cells. Emodin could increase the expression level of LC3B in HONE-1 and CNE-2 cells and improve autophagy activity. Compared with the control group, the experimental group exhibited elevated expression levels of EMT-associated protein E-cadherin, reduced expression level of vimentin, increased expression levels of autophagy-related protein 5(ATG5) and LC3B, and decreased expression level of sequestosome 1(p62). In conclusion, emodin can promote the autophagy of human nasopharyngeal carcinoma cells and inhibit the proliferation, cloning, EMT, and pelletizing ability of tumor stem cells.

Malignant tumors, as a major public health issue facing the world, have extremely complex pathogenesis. Molecular biology features represented by acidic microenvironment and lipid metabolism disorders are closely related to tumor invasion and metastasis. Traditional Chinese medicine(TCM) holds that the diseases caused by phlegm toxicity are characterized by heaviness, turbidity, stickiness, and difficulty in resolving. Modern pharmacological research has confirmed that as a TCM for resolving phlegm and detoxifying to treat phlegm toxicity, it can not only exert anti-cancer effects by directly inhibiting tumor cell proliferation but also reshape the steady-state regulatory network of the tumor microenvironment, including downregulating the microenvironment of lactate accumulation mediated by hypoxia-inducible factor 1α(HIF-1α) and regulating fatty acid synthase(FASN)-related lipid metabolism reprogramming processes, thereby antagonizing tumor invasion and metastasis. The innovative TCM theory of cancer toxicity pathogenesis proposed by our team suggests that the pathogenic characteristics of phlegm toxicity are homologous to the pathological accumulation of abnormal metabolites in the tumor microenvironment. This study systematically reviews the relevant literature on the treatment of tumors with TCM for resolving phlegm and detoxifying, deeply analyzes the theoretical basis of TCM, summarizes the clinical experience of famous TCM practitioners, and explores the clinical efficacy of TCM for resolving phlegm and detoxifying against tumors. At the same time, this study objectively summarizes its active ingredients and anti-tumor mechanisms, so as to provide reference for the prevention and treatment of malignant tumors with TCM.

Ten compounds were isolated from the 95% ethanol extract of the roots of Aconitum pendulum using silica gel chromatography, gel chromatography, and semi-preparative high-performance liquid chromatography. Their structures were identified by various spectroscopic methods, including UV, IR, HR-ESI-MS, and NMR. The compounds were identified as two phenolic compounds: 4-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyloxy]-3-methoxypropiophenone(1) and corchoionoside C(2); two lignans:(+)-oxabicyclooctalignan(3) and(-)-oxabicyclooctalignan(4); five diterpenoid alkaloids: benzoylaconine(5), 14-benzoyl-8-O-methylaconine(6),(-)-(A-b)-14α-benzoyloxy-N-ethyl-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxyaconitane(7), smirnotine A(8), and aconitine(9); and one coumarin compound: scopoletin(10). Among them, compound 1 is a novel phenolic glycoside, while compounds 3, 4, 7, 8, and 10 were isolated from A. pendulum for the first time. The anti-inflammatory activities of the compounds were evaluated using an LPS-induced RAW264.7 macrophage inflammation model. Compounds 5, 6, and 10 inhibited LPS-induced nitric oxide(NO) production in the cells, with IC_(50) values of(34.1±1.3),(28.0±1.0), and(20.3±1.7) μmol·L~(-1), respectively.