Pub Date : 2022-05-21DOI: 10.51620/0869-2084-2022-67-5-277-285
D. I. Murashka, A. Tahanovich, M. M. Kauhanka, O. Gotko, V. Prokhorova
Non-small cell lung cancer (NSCLC) occupies the first place in the structure of mortality due to oncological diseases. Late diagnosis worsens the effectiveness of its treatment. There are no informative biomarkers that allow us to judge the prevalence of the tumor process, especially in the early stages of NSCLC. To determine the level of CXCL5, CXCL8, CXCR1 and CXCR2 in the peripheral blood of patients with NSCLC to assess the possibility of their use in the diagnosis of the disease. The material was the blood of 218 patients with NSCLC, 19 patients with lung hamartoma and 42 healthy people. The concentration of CXCL5, CXCL8, and SCC in blood serum was determined by enzyme immunoassay, the CYFRA 21-1 level was determined by immunochemiluminescence analysis. The proportion of leukocytes equipped with CXCR1 and CXCR2 receptors and the fluorescence intensity of receptor complexes with antibodies (MFI) in them were measured by flow cytometry. MFI CXCR1 in granulocytes and the proportion of lymphocytes supplied CXCR2, increased in the blood already at stage I of NSCLC and showed an even more significant increase in subsequent stages. The level of these indicators was correlatively related to the stages and characteristics of NSCLC. Measuring the level of MFI CXCR1 in the blood serum makes it possible to diagnose the early stages of NSCLC with a sensitivity of 87.4% (specificity - 73.8%). Determination of the proportion of lymphocytes equipped with CXCR2 demonstrates comparable diagnostic sensitivity (87.2%) and specificity of 66.7% in the detection of stages I-II of NSCLC. MFI CXCR1 in granulocytes can also be used to differentiate stages I and II of NSCLC (diagnostic sensitivity - 75,3%, specificity - 69,6%). The sensitivity of determining for this purpose the proportion of lymphocytes equipped with CXCR2 is 75.0% with a specificity of 71.7%. In 89.7% of patients with stages III-IV NSCLC, the MFI CXCR1 in granulocytes exceeds the threshold value of 47.8 (specificity - 74.8%). Diagnostic sensitivity of determining the proportion of lymphocytes for this purpose was 90.7%.
{"title":"On the issue of diagnostic value of determining the level of receptors and their ligands in blood in non-small cell lung cancer.","authors":"D. I. Murashka, A. Tahanovich, M. M. Kauhanka, O. Gotko, V. Prokhorova","doi":"10.51620/0869-2084-2022-67-5-277-285","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-5-277-285","url":null,"abstract":"Non-small cell lung cancer (NSCLC) occupies the first place in the structure of mortality due to oncological diseases. Late diagnosis worsens the effectiveness of its treatment. There are no informative biomarkers that allow us to judge the prevalence of the tumor process, especially in the early stages of NSCLC. To determine the level of CXCL5, CXCL8, CXCR1 and CXCR2 in the peripheral blood of patients with NSCLC to assess the possibility of their use in the diagnosis of the disease. The material was the blood of 218 patients with NSCLC, 19 patients with lung hamartoma and 42 healthy people. The concentration of CXCL5, CXCL8, and SCC in blood serum was determined by enzyme immunoassay, the CYFRA 21-1 level was determined by immunochemiluminescence analysis. The proportion of leukocytes equipped with CXCR1 and CXCR2 receptors and the fluorescence intensity of receptor complexes with antibodies (MFI) in them were measured by flow cytometry. MFI CXCR1 in granulocytes and the proportion of lymphocytes supplied CXCR2, increased in the blood already at stage I of NSCLC and showed an even more significant increase in subsequent stages. The level of these indicators was correlatively related to the stages and characteristics of NSCLC. Measuring the level of MFI CXCR1 in the blood serum makes it possible to diagnose the early stages of NSCLC with a sensitivity of 87.4% (specificity - 73.8%). Determination of the proportion of lymphocytes equipped with CXCR2 demonstrates comparable diagnostic sensitivity (87.2%) and specificity of 66.7% in the detection of stages I-II of NSCLC. MFI CXCR1 in granulocytes can also be used to differentiate stages I and II of NSCLC (diagnostic sensitivity - 75,3%, specificity - 69,6%). The sensitivity of determining for this purpose the proportion of lymphocytes equipped with CXCR2 is 75.0% with a specificity of 71.7%. In 89.7% of patients with stages III-IV NSCLC, the MFI CXCR1 in granulocytes exceeds the threshold value of 47.8 (specificity - 74.8%). Diagnostic sensitivity of determining the proportion of lymphocytes for this purpose was 90.7%.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"22 1","pages":"277-285"},"PeriodicalIF":0.0,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86276235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-21DOI: 10.51620/0869-2084-2022-67-5-315-320
Y. Bocharova, T. Savinova, A. Lyamin, O. Kondratenko, S. Polikarpova, S. Zhilina, N. Fedorova, S. Semykin, A. V. Chaplin, D. Korostin, N. A. Mayansky, I. Chebotar
Stenotrophomonas maltophilia is a common opportunistic microorganism and an important respiratory pathogen in cystic fibrosis (CF). The aim of this study was to determine antimicrobial resistance phenotypes, sequence-types (ST) and genetic determinants of antibiotic resistance in S. maltophilia strains recovered from CF patients in Russia. S. maltophilia isolates recovered from 170 CF patients were analyzed. Minimum inhibitory concentrations of antibacterial agents were determined using Sensititre Gram Negative GNX2F plates and the results were interpreted according to Clinical and Laboratory Standards Institute (CLSI) criteria. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, Integrall and PubMLST were used for analysis of WGS data. S. maltophilia strains were identified from 24/170 (14%) CF patients. In total, 25 isolates were detected, two strains were isolated from the same patient. The isolates belonged to 17 different STs, including 5 new STs; ST4 was the most prevalent ST. Resistance to ceftazidime was observed in 60% of strains, to ticarcillin-clavulanate - in 32%, to levofloxacin - in 24%, to trimethoprim/sulfamethoxazole - in 12% of strains. All isolates were susceptible to minocycline. All ST4 isolates were resistant or intermediate to ceftazidime and ticarcillin-clavulanate. In two isolates, the sul1 gene was detected. In one isolate, sul1 was part of a class 1 integron. The detected integron also contained the blaGES-7 and aac(6')-Ib-cr genes. The ST4 sequence-type was the most prevalent ST among S. maltophilia strains recovered from CF patients in Russia. Antibiotic resistance genes, including sul1, blaGES-7, aac(6')-Ib-cr, were detected in single strains.
{"title":"Characteristics of Stenotrophomonas maltophilia isolates from cystic fibrosis patients in Russia.","authors":"Y. Bocharova, T. Savinova, A. Lyamin, O. Kondratenko, S. Polikarpova, S. Zhilina, N. Fedorova, S. Semykin, A. V. Chaplin, D. Korostin, N. A. Mayansky, I. Chebotar","doi":"10.51620/0869-2084-2022-67-5-315-320","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-5-315-320","url":null,"abstract":"Stenotrophomonas maltophilia is a common opportunistic microorganism and an important respiratory pathogen in cystic fibrosis (CF). The aim of this study was to determine antimicrobial resistance phenotypes, sequence-types (ST) and genetic determinants of antibiotic resistance in S. maltophilia strains recovered from CF patients in Russia. S. maltophilia isolates recovered from 170 CF patients were analyzed. Minimum inhibitory concentrations of antibacterial agents were determined using Sensititre Gram Negative GNX2F plates and the results were interpreted according to Clinical and Laboratory Standards Institute (CLSI) criteria. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, Integrall and PubMLST were used for analysis of WGS data. S. maltophilia strains were identified from 24/170 (14%) CF patients. In total, 25 isolates were detected, two strains were isolated from the same patient. The isolates belonged to 17 different STs, including 5 new STs; ST4 was the most prevalent ST. Resistance to ceftazidime was observed in 60% of strains, to ticarcillin-clavulanate - in 32%, to levofloxacin - in 24%, to trimethoprim/sulfamethoxazole - in 12% of strains. All isolates were susceptible to minocycline. All ST4 isolates were resistant or intermediate to ceftazidime and ticarcillin-clavulanate. In two isolates, the sul1 gene was detected. In one isolate, sul1 was part of a class 1 integron. The detected integron also contained the blaGES-7 and aac(6')-Ib-cr genes. The ST4 sequence-type was the most prevalent ST among S. maltophilia strains recovered from CF patients in Russia. Antibiotic resistance genes, including sul1, blaGES-7, aac(6')-Ib-cr, were detected in single strains.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"46 1","pages":"315-320"},"PeriodicalIF":0.0,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88936564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-21DOI: 10.51620/0869-2084-2022-67-5-296-300
A. S. Pimenova, N. Gadua, O. Borisova, A. Mironov, M. Afanasiev, S. Afanasiev
The results of evaluating the effectiveness of C. diphtheriae inoculation using different types of dry swabs in studies simulating various conditions of its storage at the preanalytical stage of a laboratory study for diphtheria are presented. A typical toxigenic strain of C. diphtheriae biovar gravis No. 665 was used. A commercial dry, sterile cotton swab probe (Ningbo Greetmed Medical Instruments Co., LTD, China), a commercial dry, sterile swab probe (plastic and viscose) (COPAN, Italy), tufters with a fluffy probe-tampon on a polystyrene applicator, standard (DELTALAB, SL, Spain). The tampons were pooled with a 24-hour bacterial culture of C. diphtheriae, then immediately seeded on Tellurite-containing blood agar and Corynebacagar. Storage conditions were simulated for 3 hours: at room conditions +(20-25)°C, in the refrigerator +(4-8)°C, in a thermostat +(37±1)°C. Optimal storage of C. diphtheriae on all three types of dry swabs at + (4-8) ° C; at +(20-25)° C - growth is observed when seeding from a cotton swab; in a swab with a fleecy probe-tampon, a decrease in the inoculation of C. diphtheriae was noted; when using a viscose swab - a significant loss of C. diphtheriae. At +(37±1)°C, a significant decrease in the inoculation of C. diphtheriae on all three types of tampons was noted, up to the absence of growth when using a viscose tampon. To exclude the loss of C. diphtheriae, it is necessary to observe the conditions for taking and storing biological material at the preanalytical stage of a laboratory study, which will improve the quality of laboratory microbiological studies for diphtheria infection.
{"title":"Effect of different types of dry swabs and their storage conditions on the inoculation of Corynebacterium diphtheriae.","authors":"A. S. Pimenova, N. Gadua, O. Borisova, A. Mironov, M. Afanasiev, S. Afanasiev","doi":"10.51620/0869-2084-2022-67-5-296-300","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-5-296-300","url":null,"abstract":"The results of evaluating the effectiveness of C. diphtheriae inoculation using different types of dry swabs in studies simulating various conditions of its storage at the preanalytical stage of a laboratory study for diphtheria are presented. A typical toxigenic strain of C. diphtheriae biovar gravis No. 665 was used. A commercial dry, sterile cotton swab probe (Ningbo Greetmed Medical Instruments Co., LTD, China), a commercial dry, sterile swab probe (plastic and viscose) (COPAN, Italy), tufters with a fluffy probe-tampon on a polystyrene applicator, standard (DELTALAB, SL, Spain). The tampons were pooled with a 24-hour bacterial culture of C. diphtheriae, then immediately seeded on Tellurite-containing blood agar and Corynebacagar. Storage conditions were simulated for 3 hours: at room conditions +(20-25)°C, in the refrigerator +(4-8)°C, in a thermostat +(37±1)°C. Optimal storage of C. diphtheriae on all three types of dry swabs at + (4-8) ° C; at +(20-25)° C - growth is observed when seeding from a cotton swab; in a swab with a fleecy probe-tampon, a decrease in the inoculation of C. diphtheriae was noted; when using a viscose swab - a significant loss of C. diphtheriae. At +(37±1)°C, a significant decrease in the inoculation of C. diphtheriae on all three types of tampons was noted, up to the absence of growth when using a viscose tampon. To exclude the loss of C. diphtheriae, it is necessary to observe the conditions for taking and storing biological material at the preanalytical stage of a laboratory study, which will improve the quality of laboratory microbiological studies for diphtheria infection.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"58 1","pages":"296-300"},"PeriodicalIF":0.0,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90461125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-21DOI: 10.51620/0869-2084-2022-67-5-267-270
S. Aleksanin, N. A. Alkhutova, O. L. Zhizhina, N. A. Kovyazina, N. V. Nesterenko, V. Rybnikov
The complex of climatic and geographical conditions of the Arctic determines the high intensity of the polysystem adaptive response of the organism, the duration of which is additionally influenced by individual genetic characteristics, social conditions, psychological and work loads. Taking into account the relevance of timely prevention and early diagnosis of stress-induced somatic pathology in EMERCOM employees working in unfavorable climatic and geographical zones, the authors evaluated the informative value of determining the level of steroid hormones and insulin as laboratory markers of adaptation to Arctic conditions. The expediency of developing objective criteria for the interpretation of the insulin/cortisol index and studying the informativeness of the 17ONprogesterone level as the earliest marker of adaptation to unfavorable climatic and geographical conditions of the Arctic is substantiated.
{"title":"Laboratory markers of adaptation to Arctic conditions: scientific search and prospects of implementation.","authors":"S. Aleksanin, N. A. Alkhutova, O. L. Zhizhina, N. A. Kovyazina, N. V. Nesterenko, V. Rybnikov","doi":"10.51620/0869-2084-2022-67-5-267-270","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-5-267-270","url":null,"abstract":"The complex of climatic and geographical conditions of the Arctic determines the high intensity of the polysystem adaptive response of the organism, the duration of which is additionally influenced by individual genetic characteristics, social conditions, psychological and work loads. Taking into account the relevance of timely prevention and early diagnosis of stress-induced somatic pathology in EMERCOM employees working in unfavorable climatic and geographical zones, the authors evaluated the informative value of determining the level of steroid hormones and insulin as laboratory markers of adaptation to Arctic conditions. The expediency of developing objective criteria for the interpretation of the insulin/cortisol index and studying the informativeness of the 17ONprogesterone level as the earliest marker of adaptation to unfavorable climatic and geographical conditions of the Arctic is substantiated.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"37 1","pages":"267-270"},"PeriodicalIF":0.0,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88648064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-21DOI: 10.51620/0869-2084-2022-67-5-286-291
R. Rahimova
Autoimmune thyroiditis is a group of organ-specific autoimmune thyropathies, which are caused by a genetically determined defect in immune tolerance to thyroid antigens, as a result of which its autoimmune damage occurs. The aim of the study was to analyze literature data on the pathogenetic role of genetic and biochemical parameters in patients with autoimmune thyroiditis.
{"title":"Autoimmune thyroiditis (review of literature).","authors":"R. Rahimova","doi":"10.51620/0869-2084-2022-67-5-286-291","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-5-286-291","url":null,"abstract":"Autoimmune thyroiditis is a group of organ-specific autoimmune thyropathies, which are caused by a genetically determined defect in immune tolerance to thyroid antigens, as a result of which its autoimmune damage occurs. The aim of the study was to analyze literature data on the pathogenetic role of genetic and biochemical parameters in patients with autoimmune thyroiditis.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"29 12","pages":"286-291"},"PeriodicalIF":0.0,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72412935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-17DOI: 10.51620/0869-2084-2022-67-4-219-226
A. Pursanova, L. N. Kazarina, I. Kruglova, S. Zinovev, O. Utkin, E. Filatova
Purpose of the study improving the diagnosis of precancerous diseases and cancer of the oral mucosa using fluorescent immunocytochemical studies by direct immunofluorescence. A clinical laboratory examination of 111 patients was carried out: 46 patients with squamous cell carcinoma of the oral mucosa, 35 people with precancerous lesions (17 leukoplakia, 18 - oral lichen planus) and 30 healthy people. All patients underwent a traditional cytological examination and an additional immunocytochemical examination by direct immunofluorescence, the expression levels of tumor markers P53, P16 and Ki67 were determined. The data were compared with the results of histological analysis. As a result of the study, it was revealed that in patients with cancer, the expression of oncomarker P53 was four times higher than in patients with precancerous pathology. In 6.52% of cases, co-expression of markers Ki67 and P16 was found. Thus, the advantages of fluorescent immunocytochemical diagnostics were the absence of invasive traumatic intake of the biomaterial in the patient, reduction in the timing of obtaining the result, high sensitivity, and the possibility of remote evaluation of the results. Therefore, that increases the accessibility of the method, and the possibility of using this method for a screening study of population.
{"title":"The fluorescent immunocytochemical diagnostics of precancerous diseases and cancer of the oral mucosa.","authors":"A. Pursanova, L. N. Kazarina, I. Kruglova, S. Zinovev, O. Utkin, E. Filatova","doi":"10.51620/0869-2084-2022-67-4-219-226","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-4-219-226","url":null,"abstract":"Purpose of the study improving the diagnosis of precancerous diseases and cancer of the oral mucosa using fluorescent immunocytochemical studies by direct immunofluorescence. A clinical laboratory examination of 111 patients was carried out: 46 patients with squamous cell carcinoma of the oral mucosa, 35 people with precancerous lesions (17 leukoplakia, 18 - oral lichen planus) and 30 healthy people. All patients underwent a traditional cytological examination and an additional immunocytochemical examination by direct immunofluorescence, the expression levels of tumor markers P53, P16 and Ki67 were determined. The data were compared with the results of histological analysis. As a result of the study, it was revealed that in patients with cancer, the expression of oncomarker P53 was four times higher than in patients with precancerous pathology. In 6.52% of cases, co-expression of markers Ki67 and P16 was found. Thus, the advantages of fluorescent immunocytochemical diagnostics were the absence of invasive traumatic intake of the biomaterial in the patient, reduction in the timing of obtaining the result, high sensitivity, and the possibility of remote evaluation of the results. Therefore, that increases the accessibility of the method, and the possibility of using this method for a screening study of population.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"1 1","pages":"219-226"},"PeriodicalIF":0.0,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88769337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-17DOI: 10.51620/0869-2084-2022-67-4-237-243
V. N. Gerasimov, N. N. Marinina, G. G. Kharseeva, O. S. Sherbataya
Determination of changes in the morphostructure of microorganisms under the influence of antibiotics, disinfectants, biocins, physical and chemical agents is carried out using the method of visualization of ultrathin sections in a transmission electron microscope. The aim is to develop methodological techniques for express preparation and visualization of the ultrastructure of bacteria in a transmission electron microscope to detect structural damage to cells and assess the quality of the microbial population. A technique of accelerated sample preparation for visualization of the fine structure of bacteria and diagnosis of structural damage to cells in a transmission electron microscope has been developed. The use of this method will make it possible to more effectively investigate the mechanisms of inactivation of microorganisms at different stages of contact with antibiotics, disinfectants, biocines, including various stressful, damaging and lethal physical and chemical effects.
{"title":"Possibilities of transmission electron microscopy in assessing the quality of bacterial cells and microbial population.","authors":"V. N. Gerasimov, N. N. Marinina, G. G. Kharseeva, O. S. Sherbataya","doi":"10.51620/0869-2084-2022-67-4-237-243","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-4-237-243","url":null,"abstract":"Determination of changes in the morphostructure of microorganisms under the influence of antibiotics, disinfectants, biocins, physical and chemical agents is carried out using the method of visualization of ultrathin sections in a transmission electron microscope. The aim is to develop methodological techniques for express preparation and visualization of the ultrastructure of bacteria in a transmission electron microscope to detect structural damage to cells and assess the quality of the microbial population. A technique of accelerated sample preparation for visualization of the fine structure of bacteria and diagnosis of structural damage to cells in a transmission electron microscope has been developed. The use of this method will make it possible to more effectively investigate the mechanisms of inactivation of microorganisms at different stages of contact with antibiotics, disinfectants, biocines, including various stressful, damaging and lethal physical and chemical effects.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"23 4","pages":"237-243"},"PeriodicalIF":0.0,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72471882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-17DOI: 10.51620/0869-2084-2022-67-4-234-236
A. Yovdiy, E. Butina, E. Poponina
Timely diagnostics of red blood cells (RBC) antibodies and selection of immunologically compatible donors can prevent the occurrence of hemolytic transfusion reactions in recipients of RBC. The article presents an analysis of a clinical case describing the variability of the antibody screening results and the difficulties of individual selection of donors for a alloimmunized patient with myelodysplastic syndrome. The basic immunohematological principles have been formulated to ensure the safety of transfusions in patients with RBC antibodies.
{"title":"Immunohematological provision of red blood cells transfusions to alloimmunized recipients (clinical case).","authors":"A. Yovdiy, E. Butina, E. Poponina","doi":"10.51620/0869-2084-2022-67-4-234-236","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-4-234-236","url":null,"abstract":"Timely diagnostics of red blood cells (RBC) antibodies and selection of immunologically compatible donors can prevent the occurrence of hemolytic transfusion reactions in recipients of RBC. The article presents an analysis of a clinical case describing the variability of the antibody screening results and the difficulties of individual selection of donors for a alloimmunized patient with myelodysplastic syndrome. The basic immunohematological principles have been formulated to ensure the safety of transfusions in patients with RBC antibodies.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"12 1","pages":"234-236"},"PeriodicalIF":0.0,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86297821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-17DOI: 10.51620/0869-2084-2022-67-4-227-233
Yuliya Aleksandrovna Akinshina, S. Mardanly, S. Rotanov, V. V. Pomazanov, V. A. Kiseleva, I. I. Ermolaev
The paper present the results of a survey of people who have undergone immunization with a combined vector vaccine for the prevention of coronavirus infection COVID-19 «Sputnik V - Gam-COVID-Vac», as well as COVID-19 recovalents. Using a quantitative enzyme-linked immunosorbent assay, the levels of specific IgG were determined in persons who had had different degrees of severity before vaccination, in persons who were immuno-negative before immunization, as well as in convalescents who had undergone coronavirus infection of varying severity. The immunological targeting of antibodies against various SARS-CoV-2 proteins is considered.
本文介绍了预防冠状病毒感染的COVID-19“Sputnik V - Gam-COVID-Vac”联合载体疫苗免疫接种人群以及COVID-19恢复价的调查结果。采用定量酶联免疫吸附试验，对接种疫苗前有不同严重程度的人、接种疫苗前免疫阴性的人以及不同严重程度的冠状病毒感染的康复者进行特异性IgG水平测定。考虑了针对各种SARS-CoV-2蛋白的抗体的免疫靶向。
{"title":"Features of the humoral response to immunization \"Gam-COVID-Vac\" and in patients with COVID-19.","authors":"Yuliya Aleksandrovna Akinshina, S. Mardanly, S. Rotanov, V. V. Pomazanov, V. A. Kiseleva, I. I. Ermolaev","doi":"10.51620/0869-2084-2022-67-4-227-233","DOIUrl":"https://doi.org/10.51620/0869-2084-2022-67-4-227-233","url":null,"abstract":"The paper present the results of a survey of people who have undergone immunization with a combined vector vaccine for the prevention of coronavirus infection COVID-19 «Sputnik V - Gam-COVID-Vac», as well as COVID-19 recovalents. Using a quantitative enzyme-linked immunosorbent assay, the levels of specific IgG were determined in persons who had had different degrees of severity before vaccination, in persons who were immuno-negative before immunization, as well as in convalescents who had undergone coronavirus infection of varying severity. The immunological targeting of antibodies against various SARS-CoV-2 proteins is considered.","PeriodicalId":52451,"journal":{"name":"Klinichescheskaya Laboratornaya Diagnostika","volume":"61 1","pages":"227-233"},"PeriodicalIF":0.0,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90041610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-17DOI: 10.51620/0869-2084-2022-67-4-244-249
A. Anisimova, M. V. Poleeva, N. Aronova, M. V. Tsimbalistova, N. V. Pavlovich
To carry the comparative analysis of sample preparation methods for the most effective identification of Candida yeast by mass spectrometric analysis. 265 strains of yeast and yeast-like fungi isolated from the sputum of patients with pneumonia were investigated. The selected strains were identified by conventional methods (cultural, morphological, tinctorial, enzymatic properties) and MALDI-ToF MS using the Autoflex speed III Bruker Daltonics mass spectrometer (Germany) and Flex Control software. To evaluate the effectiveness of fungi species determinination, the comparative analysis of sample preparation was performed using 4 methods: direct application to the target, an extended direct application method, protein extraction using ethanol/formic acid or trifluoroacetic acid. The accelerated scheme of identification of fungi by the culture method does not provide clear and unambiguous results. When using mass spectrometric analysis, the reliability of the results depended on the sample preparation. A comparative study of the effectiveness of fungi species determination by various methods of the sample preparation of 50 clinical isolates was carried out. It was revealed that the extraction of cells using TFC acid does not lead to the appearance of the recordable protein spectra. The use of direct and extended direct application methods made it possible to establish the species only in 32-44% of the strains. The most effective method of sample preparation was the method using formic acid and ethanol, which allowed us to determine the species affiliation in 100% of the studied fungi (Score 2.0). Depending on the yeast species, a high statistical indicator (Score≥2.3) was registered for 42-100% of samples. The results of present study show that the use of MALDI-ToF MS is the most reliable and informative method of Candida spp.identification.
对质谱法鉴定念珠菌最有效的样品制备方法进行比较分析。对从肺炎患者痰液中分离的265株酵母菌和酵母菌样真菌进行了调查。所选菌株采用常规方法(培养、形态学、染色、酶学性质)和MALDI-ToF MS进行鉴定，使用Autoflex speed III Bruker Daltonics质谱计(德国)和Flex Control软件。为评价真菌种类测定的有效性，采用直接作用于靶点、扩展直接作用于靶点、乙醇/甲酸和三氟乙酸提取蛋白质4种方法对样品制备进行对比分析。用培养法鉴定真菌的加速方案不能提供明确和明确的结果。当使用质谱分析时，结果的可靠性取决于样品的制备。对50株临床分离菌不同制样方法的菌种测定效果进行了比较研究。结果表明，使用TFC酸提取细胞不会导致可记录的蛋白质光谱的出现。采用直接和扩展直接施用的方法，只在32-44%的菌株中建立了菌种。最有效的样品制备方法是使用甲酸和乙醇的方法，该方法使我们能够在100%的研究真菌中确定物种归属(得分2.0)。根据酵母菌种类的不同，42-100%的样品具有高统计指标(得分≥2.3)。本研究结果表明，利用MALDI-ToF质谱法鉴定念珠菌属是最可靠、信息最丰富的方法。
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